MDA-MB-231 Coculture with M1 Macrophages, sample 1
source_name
MDA-MB-231 and THP-1
cell line
MDA-MB-231 and THP-1
cell type
triple negative breast cancer cell line and M1-like macrophage polarized peripheral blood monocyte THP-1 cells
treatment
Following 24 hours of stimulation with 100 nM PMA in D-MEM/F, THP-1 cells were activated for 24 hours with 50 ng/mL ifn-γ to induce an M1-macrophage state. 10,000 MDA-MB-231 cells were seeded into each well of a Nunclon Sphera-treated U-shaped-bottom microplate along with 5,000 polarized M1-like macrophages cells and co-cultured for 4 days at 37 °C in a humidified incubator with 5% CO2.
geo_loc_name
missing
collection_date
missing