MIGS Eukaryotic sample from Cryptomonas
isolate
UKO-2021-05-05
collection_date
2021-05-05
depth
0.5 meters
env_broad_scale
Tropical | marine | lagoon
env_local_scale
enbayment
env_medium
estuarine water
geo_loc_name
Puerto Rico: La Parguera Bioluminescent Bay
isol_growth_condt
The culture was founded from a single cell isolated on 2021-05-05 from a water sample collected in the La Parguera Bioluminescent Bay, Puerto Rico. The monoculture was maintained in a 2 L Corning PYREX 1395 round media storage bottle plugged with a cotton stopper to allow gas exchange while excluding external microorganisms. The growth medium contained 1.8 L distilled water, 64.8 g Instant Ocean sea salt, 234 uL Fritz Aquatics F/2 Algae Food Part A, 234 uL Part B, and 2160 uL of a 3.33 g/L sodium selenate stock solution. Transfers were performed weekly. Kanamycin (0.306 g) was added during the first two weeks to ensure an axenic culture.
lat_lon
18 N 67 W
isolation_source
semi-enclosed embayment
BioSampleModel
MIGS.eu
BioSampleModel
MIGS/MIMS/MIMARKS.water