M_1
Protocols: Left ventricular myocardial tissue samples were collected from SD rats 24 hours after reperfusion. The heart was rapidly excised, rinsed with pre-cooled saline, and the left ventricle was dissected. Tissue samples were immediately flash-frozen in liquid nitrogen and stored at -80°C until RNA extraction. Male Sprague-Dawley rats (250-300 g, 8 weeks old) were housed in a specific pathogen-free facility under controlled conditions (22 ± 2°C, 50% ± 10% humidity, 12 h light/dark cycle) with free access to food and water. Rats were randomly assigned to three groups: Sham (Vehicle), I/R (ischemia-reperfusion injury model), and I/R+BAI (baicalin treatment). The I/R model was established by 45 min of left anterior descending coronary artery ligation followed by 24 h of reperfusion. Baicalin (100 mg/kg) was administered via intraperitoneal injection 10 minutes prior to reperfusion. The Vehicle group received an equivalent volume of saline containing 1% CMC-Na. This protocol is not applicable for RNA-seq data as no data format conversion was performed. Total RNA was extracted from myocardial tissue using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer. Only samples with RNA Integrity Number (RIN) greater than 7.0 were used for subsequent library preparation. RNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit (NEB) following the manufacturer's instructions. Briefly, mRNA was enriched from total RNA using oligo-dT magnetic beads, fragmented, and reverse-transcribed into cDNA. After end-repair, adapter ligation, and PCR amplification, the final cDNA libraries were quality-checked.
ENA first public
2026-08-05
INSDC center name
Women and Children's Hospital of Ningbo University
INSDC status
public
Submitter Id
E-MTAB-17471:M_1
age
8
biological sex
male
broker name
ArrayExpress
cell type
cardiomyocyte
collection date
not collected
common name
Norway rat
developmental stage
adult
disease
myocardial ischemia
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
3
isolate
not applicable
organism part
heart
sample name
E-MTAB-17471:M_1
scientific_name
Rattus norvegicus
stimulus
Ischemia-reperfusion injury
strain
Sprague-Dawley