BatSui3
Protocols: Adult Bathyergus suillus were wild-captured in Darling, Western Cape, South Africa using Hickman live traps baited with small pieces of sweet potato. Traps were monitored every 2–3 hours throughout the day and checked again early each morning after being left overnight. Permission to capture animals was obtained from all relevant landowners, and collection permits were issued by the appropriate conservation authorities (Western Cape permit no. CN44-87-13780). All procedures were approved by the University of Pretoria Animal Use and Care Committee (ethics clearance no. NAS022/2021) and conducted under DAFF Section 20 approval (SDAH-Epi-21051907211). Captured animals were transported to the University of Pretoria and housed individually at approximately 23°C, 21% O₂, and 50% relative humidity under a 12 h light:12 h dark photoperiod. Animals were maintained in large polyurethane crates (1 × 0.5 × 0.5 m) containing wood shavings and paper towelling as nesting material in temperature-controlled rooms. All wild-caught African mole-rat species were acclimated to captivity for a minimum of one year prior to tissue collection. Animals were euthanized by cervical dislocation and decapitation. Immediately after death, biological tissues were rapidly dissected within 60 s and flash-frozen in liquid nitrogen. Samples were subsequently stored at −80 °C until further analysis. Ultra-high molecular weight DNA was extracted from the spleen tissue of a female, a male, and their male pup using a modified phenol/chloroform DNA extraction method. Long-read whole genome sequencing was performed at The Centre for Applied Genomics (TCAG) in Toronto, Canada.  7 ug of DNA of each sample was sheared to 15-20 kb fragments by g-TUBE (Covaris, Woburn, MA), followed by library preparation using the SMRTbell Express Template Prep Kit 3.0 (Pacific Biosciences, Menlo Park, CA) using the manufacturer's recommended protocol. Each library was size selected at 10 kb by Bluepippin (Sage Science, Beverly, MA).
ENA first public
2026-08-05
INSDC center name
The Hospital for Sick Children, Toronto Ontario, Canada The Ontario Institute for Cancer Research, Toronto, Ontario, Canada
INSDC status
public
Submitter Id
E-MTAB-17312:BatSui3
age
unknown
broker name
ArrayExpress
collection date
not collected
common name
Cape dune mole-rat
developmental stage
adult
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
BatSui3
isolate
not applicable
organism part
kidney
sample name
E-MTAB-17312:BatSui3
scientific_name
Bathyergus suillus
sex
male
strain
wild-derived