mHetGla4_male_run2
Protocols: A single 1 year old male naked mole-rat was harvested for mHetGla4, where a liver was harvested and flash-frozen in isopentane on dry ice.  High molecular weight DNA was extracted from frozen liver with the NEB Monarch cells and tissue kit, following the manufacturer's instructions. Long-read whole genome sequencing was performed at Network Biology Collaborative Center (NBCC), at Mount Sinai Hospital in Toronto, Ontario. The gDNA quality was accessed by Agilent Fragment Analyzer 5200.The high-quality gDNA with GQN > 7.0 was first cleaned up with PacBio Short Read Eliminator (cat#102-208-300) according to the manufacturer protocol. It was then sheared with Covaris g-TUBE (cat# 520079) and the SMRTbell HiFi library was prepared using PacBio SMRTBell Prep Kit 3.0 (cat#102-182-700). The quality and quantity of the SMRTbell HiFi Library were measured by Agilent Fragment Analyzer and Qubit 2.0 (Thermo Fisher Scientific), respectively. The final polymerase-bind library was prepared with ABC (annealing, binding, and cleanup) procedure according to the SMRTLink software instruction using Revio Polymerase Kit (Cat#102-739-100).
ENA first public
2026-08-05
INSDC center name
The Hospital for Sick Children, Toronto Ontario, Canada The Ontario Institute for Cancer Research, Toronto, Ontario, Canada
INSDC status
public
Submitter Id
E-MTAB-17313:mHetGla4_male_run2
age
4
broker name
ArrayExpress
collection date
not collected
common name
naked mole-rat
developmental stage
adult subordinate
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
NMRmHetGla4
isolate
not applicable
organism part
kidney
sample ID
m84134_240510_185452_s1
sample name
E-MTAB-17313:mHetGla4_male_run2
scientific_name
Heterocephalus glaber
sex
male
strain
Ansorgei