Untreated 0-h alfalfa leaf disk, biological replicate 2
Untreated 0-h baseline sample of Medicago sativa cv. Zhongmu No. 1 leaf disks, biological replicate 2. WT denotes the untreated baseline and not a distinct genotype.
cultivar
Zhongmu No. 1
age
3-4 weeks
dev_stage
seedling stage
collection_date
2023
geo_loc_name
China: Shaanxi, Xi'an
tissue
leaf
biomaterial_provider
School of Life Sciences, Northwestern Polytechnical University
growth_protocol
Surface-sterilized seeds were sown on half-strength MS solid medium, stratified at 4 degree Celsius in the dark for 2-3 days, and grown at 22 degree Celsius under a 16 h light/8 h dark photoperiod with a light intensity of 120-150 micromol m-2 s-1 until 3-4 weeks old.
isolation_source
leaf disk excised from the first trifoliate leaf
sample_type
plant tissue
temp
22 degree Celsius
treatment
Untreated 0-h baseline; freshly excised leaf disks collected immediately before transfer to liquid culture.
time_point
0 h
replicate
2
BioSampleModel
Plant