Dkk1 MCAO
Protocols: In adult Rosa26-tdTomato/Cspg4-CreERT2 male mice carrying Rosa26-Dkk1 allele, middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Three days after surgery, the cerebral cortex adjacent to the injury site was isolated. For healthy tissue (CTRL), sham-operated mice were used as controls. Fluorescence-activated cell sorting (FACS) was employed to isolate viable cells that were CD31/45-negative and CellTrace-calcein green-positive, expressing tdTomato/NG2 red fluorescence. For each sample, we used tdTomato/NG2 cell isolated from 8 biological replicates. cDNA was extracted from cell suspension during library preparation routine using Chromium next gem single-cell 3′ reagent kit (version 3.1). Single-cell RNA-seq libraries were prepared using Chromium controller instrument and Chromium Next Gem single-cell 3' reagent kit version 3.1 (both 10X Genomics, Pleasanton, CA, USA) according to the manufacturer's protocol targeting 4000 cells per sample.
ENA first public
2026-08-06
INSDC center name
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC status
public
Submitter Id
E-MTAB-14905:Dkk1 MCAO
age
50 to 56
broker name
ArrayExpress
cell_type
tdTomato/NG2-positive viable cells, CD31/45-negative
collection date
not collected
common name
house mouse
developmental stage
adult
genotype
Rosa26-Dkk1/Rosa26-tdTomato/Cspg4-CreERT2
geographic location (country and/or sea)
not collected
individual
9-16
injury
middle cerebral artery occlusion
isolate
not applicable
organism part
brain cortex
sample name
E-MTAB-14905:Dkk1 MCAO
scientific_name
Mus musculus
sex
male
strain
C567BL/6