mHetGla4_male_brain
Protocols: Naked mole-rats were bred in-house and maintained in stable colonies (i.e. established breeders were present) housed in large (45.75 cm L × 24 cm W × 15.25 cm H) and small (30 cm L × 18 cm W × 13 cm H) polycarbonate cages connected by plastic tubes (25 cm L × 5 cm D). Animals were fed sweet potato ad libitum supplemented with wet 19% protein mash (Envigo RMS, Inc.) and kept on a 12:12 light/dark cycle at 28–30 C. All procedures were approved by the University Animal Care Committee and performed in accordance with federal and institutional guidelines. One male animal, mHetGla4 was anesthetized with isoflurane and rapidly decapitated before the brain and kidney was harvested and flash-frozen on dry ice. NA was extracted using the Monarch HMW tissue extraction kit according to manufacturers recommendations (New England Biolabs). High molecular weight genomic DNA was prepared for sequencing using the Ligation Sequencing Kit V14 (SQK-LSK114, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturer's protocol. Briefly, 10 µg of genomic DNA was repaired and dA-tailed using the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA), followed by purification with AMPure XP beads (Beckman Coulter, Brea, CA, USA). Sequencing adapters were then ligated to the repaired and dA-tailed DNA using NEBNext Quick Ligation Module (NEB) and Adapter Mix (Oxford Nanopore Technologies), and the library was purified again with AMPure XP beads using the Long Fragment Buffer (LFB) wash step to enrich for longer DNA fragments. The final library was quantified using a Qubit 4 Fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), and the appropriate amount of the prepared library was loaded onto a PromethION Flow Cell (R10.4.1, FLO-PRO114M).
ENA first public
2026-08-06
INSDC center name
The Hospital for Sick Children, Toronto Ontario, Canada The Ontario Institute for Cancer Research, Toronto, Ontario, Canada
INSDC status
public
Submitter Id
E-MTAB-17334:mHetGla4_male_brain
age
4
broker name
ArrayExpress
collection date
not collected
common name
naked mole-rat
developmental stage
adult subordinate
genotype
wild type genotype
geographic location (country and/or sea)
not collected
individual
NMRmHetGla4
isolate
not applicable
organism part
brain
sample name
E-MTAB-17334:mHetGla4_male_brain
scientific_name
Heterocephalus glaber
sex
male
strain
Ansorgei