Bikinin-treated alfalfa leaf disk at 24 h, biological replicate 3
Bikinin-treated sample of Medicago sativa cv. Zhongmu No. 1 leaf disks collected after 24 h, biological replicate 3.
cultivar
Zhongmu No. 1
age
3-4 weeks
dev_stage
seedling stage
collection_date
2023
geo_loc_name
China: Shaanxi, Xi'an
tissue
leaf
biomaterial_provider
School of Life Sciences, Northwestern Polytechnical University
growth_protocol
Surface-sterilized seeds were sown on half-strength MS solid medium, stratified at 4 degree Celsius in the dark for 2-3 days, and grown at 22 degree Celsius under a 16 h light/8 h dark photoperiod with a light intensity of 120-150 micromol m-2 s-1 until 3-4 weeks old.
isolation_source
leaf disk excised from the first trifoliate leaf
sample_type
plant tissue
temp
22 degree Celsius
treatment
Induction medium containing 0.625 mg/L 2,4-D, 0.125 mg/L kinetin, and 30 micromolar bikinin; incubated for 24 h under continuous light at 22 degree Celsius on a shaker at 100-120 rpm.
time_point
24 h
replicate
3
BioSampleModel
Plant