Rhizosphere of green cos lettuce, baseline seedlings at transplanting (day 0)
Composite rhizosphere sample from uniform green cos lettuce seedlings raised on dechlorinated tap water, collected immediately before transplanting into the hydroponic system. Serves as the common day 0 baseline for all treatments.
chem_administration
not applicable
collection_date
not collected
env_broad_scale
agricultural biome [ENVO:01001442]
env_local_scale
greenhouse [ENVO:03501307]
env_medium
rhizosphere [ENVO:01000998]
food_source
not applicable
geo_loc_name
Thailand: Khon Kaen
host
Lactuca sativa
host_age
14 days
host_common_name
green cos lettuce
host_genotype
not applicable
host_height
not collected
host_length
not collected
host_life_stage
seedling (three-true-leaf stage)
host_phenotype
not applicable
host_taxid
4236
host_tot_mass
not collected
lat_lon
16.47 N 102.82 E
microbial_biomass
not applicable
microbial_biomass_meth
not applicable
pathogenicity
not applicable
samp_store_dur
not collected
samp_store_loc
Khon Kaen University, Khon Kaen, Thailand
sieving
not applicable
soil_type
not applicable
soil_type_meth
not applicable
store_cond
frozen, -80 degree Celsius
temp
30 degree Celsius
agrochem_addition
not applicable
biotic_relationship
free living
collection_method
aseptic excision of whole root systems, pooled per treatment
crop_rotation
not applicable
cur_land_use
farmstead
cur_vegetation
Lactuca sativa monoculture
fertilizer_admin
not applicable
fungicide_regm
not applicable
genetic_mod
not applicable
herbicide_regm
not applicable
host_subspecf_genlin
variety: longifolia; cultivar: Caesar
humidity
55-85 %
isolation_source
rhizosphere of hydroponically grown green cos lettuce
pesticide_regm
not applicable
ph_meth
benchtop pH meter, daily measurement before adjustment
plant_growth_med
hydroponics: closed-loop recirculating deep flow technique (DFT), polyurethane sponge cube in net pot
plant_struc
root
plant_water_method
hydroponics
rel_to_oxygen
aerobe
root_med_macronutr
dechlorinated tap water only
root_med_ph
not applicable
salt_regm
not applicable
samp_collect_device
sterile forceps and scalpel
samp_mat_process
roots rinsed to remove loosely adhering solution; rhizoplane and adhering rhizosphere material retained; ground in liquid nitrogen
samp_pooling
one composite sample per treatment, pooled from multiple plants
samp_size
0.3 g root tissue used for DNA extraction
samp_store_temp
-80 degree Celsius
season_environment
tropical greenhouse under natural light with 70% light-transmittance shading net
soil_conductivity
not applicable
soil_horizon
not applicable
soil_ph
not applicable
soil_texture_meth
not applicable
source_material_id
FF68-D0
tillage
not applicable
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.agriculture