Rhizosphere of green cos lettuce, AB mix control, day 31
Composite rhizosphere sample from green cos lettuce grown for 31 days in a closed-loop recirculating hydroponic system supplied with conventional AB mix nutrient solution (EC 1.6 mS/cm). Control treatment.
chem_administration
AB mix hydroponic nutrient solution (KMITL Formula 2), EC 1.6 mS/cm; continuous recirculation, day 0 to day 31
collection_date
not collected
env_broad_scale
agricultural biome [ENVO:01001442]
env_local_scale
greenhouse [ENVO:03501307]
env_medium
rhizosphere [ENVO:01000998]
food_source
not applicable
geo_loc_name
Thailand: Khon Kaen
host
Lactuca sativa
host_age
45 days
host_common_name
green cos lettuce
host_genotype
not applicable
host_height
not collected
host_length
not collected
host_life_stage
vegetative (harvest stage)
host_phenotype
not applicable
host_taxid
4236
host_tot_mass
not collected
lat_lon
16.47 N 102.82 E
microbial_biomass
not applicable
microbial_biomass_meth
not applicable
pathogenicity
not applicable
samp_store_dur
not collected
samp_store_loc
Khon Kaen University, Khon Kaen, Thailand
sieving
not applicable
soil_type
not applicable
soil_type_meth
not applicable
store_cond
frozen, -80 degree Celsius
temp
30 degree Celsius
agrochem_addition
not applicable
biotic_relationship
free living
collection_method
aseptic excision of whole root systems, pooled per treatment
crop_rotation
not applicable
cur_land_use
farmstead
cur_vegetation
Lactuca sativa monoculture
fertilizer_admin
synthetic two-part hydroponic nutrient solution (AB mix), EC maintained at 1.6 mS/cm
fungicide_regm
not applicable
genetic_mod
not applicable
herbicide_regm
not applicable
host_subspecf_genlin
variety: longifolia; cultivar: Caesar
humidity
55-85 %
isolation_source
rhizosphere of hydroponically grown green cos lettuce
pesticide_regm
not applicable
ph_meth
benchtop pH meter, daily measurement before adjustment
plant_growth_med
hydroponics: closed-loop recirculating deep flow technique (DFT), polyurethane sponge cube in net pot
plant_struc
root
plant_water_method
hydroponics
rel_to_oxygen
aerobe
root_med_macronutr
Ca(NO3)2.4H2O, KNO3, KH2PO4, MgSO4, Fe-EDTA, trace element mix
root_med_ph
6.0-7.0 (adjusted daily; drifted to 8.0 between adjustments)
salt_regm
not applicable
samp_collect_device
sterile forceps and scalpel
samp_mat_process
roots rinsed to remove loosely adhering solution; rhizoplane and adhering rhizosphere material retained; ground in liquid nitrogen
samp_pooling
one composite sample per treatment, pooled from multiple plants
samp_size
0.3 g root tissue used for DNA extraction
samp_store_temp
-80 degree Celsius
season_environment
tropical greenhouse under natural light with 70% light-transmittance shading net
soil_conductivity
1.6 mS/cm (nutrient solution)
soil_horizon
not applicable
soil_ph
not applicable
soil_texture_meth
not applicable
source_material_id
FF68-ABD31
tillage
not applicable
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.agriculture