GS_PI_2
ENA first public
2026-08-19
INSDC center name
EPFL
INSDC status
public
Submitter Id
GS_PI_2
altitude
2621.4
broad-scale environmental context
mountain biome [ENVO:01000364]
collection date
2024-09-20
current vegetation
19.2
current vegetation method
percentage green coverage (photograph-based)
depth
100
elevation
2621.4
environmental medium
soil
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.411627
geographic location (longitude)
8.075225
geographic location (region and locality)
Bettmerhorn, Valais, Switzerland
local environmental context
permafrost soil environment or alpine scree
negative control type
not applicable
nucleic acid amplification
PCR amplification of the full-length 16S rRNA gene from extracted soil DNA
nucleic acid extraction
DNeasy PowerSoil Pro Kits for Soil DNA Extraction
pH
4.15
pH method
2 g of frozen soil were mixed with 5 mL 0.05 M CaCl2 solution and pH was measured with a Orion Lab Star PH111 pH/mV meter (Thermo Fisher Scientific, WA, USA)
pcr primers
bacterial 16S rRNA 22f primer 5'-AGA GTT TGA TCM TGG CTC AG-3'; 1392r primer 5'-CGG TTA CCT TGT TAC GAC TT-3'
project name
Effects of diurnal temperature cycles and elevated temperatures on CH₄ oxidation and trace gas dynamics in high-alpine soil microcosms
sample collection method
Surface soil (0–10 cm) manually collected using a sterilized shovel and transferred to sterile sampling containers.
sample name
GS_PI_2
sample storage conditions
frozen at -20°C
sample volume or weight for DNA extraction
1
scientific_name
soil metagenome
total carbon content
4.061638
total carbon method
TOC measured on acidified soil (HCl removal of inorganic carbon) using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
total nitrogen content
4,108,388
total nitrogen content method
TN measured on dried and ground soil using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
water content
38.2
water content method
Gravimetric water content was determined by drying 2 g of frozen soil at 100 °C for 24 h