PCR_nc
ENA first public
2026-08-19
INSDC center name
EPFL
INSDC status
public
Submitter Id
PCRnc
broad-scale environmental context
not provided
collection date
not provided
current vegetation method
not provided
depth
100
elevation
2808
environmental medium
not provided
geographic location (country and/or sea)
not provided
geographic location (latitude)
46.091009
geographic location (longitude)
7.59561
geographic location (region and locality)
not provided
local environmental context
not provided
negative control type
negative PCR control
nucleic acid amplification
PCR amplification of the full-length 16S rRNA gene from extracted soil DNA
nucleic acid extraction
DNeasy PowerSoil Pro Kits for Soil DNA Extraction
pH method
not provided
pcr primers
bacterial 16S rRNA 22f primer 5'-AGA GTT TGA TCM TGG CTC AG-3'; 1392r primer 5'-CGG TTA CCT TGT TAC GAC TT-3'
project name
Diurnal variability of soil CO₂ and CH₄ fluxes and microbial community dynamics in high-alpine soils
sample collection method
not provided
sample name
PCRnc
sample storage conditions
not provided
scientific_name
soil metagenome
total carbon method
not provided
total nitrogen content method
not provided
water content method
not provided