C2_w1_16
ENA first public
2026-08-19
INSDC center name
EPFL
INSDC status
public
Submitter Id
C2_w1_16
altitude
2813.8
broad-scale environmental context
mountain biome [ENVO:01000364]
collection date
2024-09-06
current vegetation
10.12
current vegetation method
percentage green coverage (photograph-based)
depth
100
elevation
2813.8
environmental medium
soil
geographic location (country and/or sea)
Switzerland
geographic location (latitude)
46.091229
geographic location (longitude)
7.595418
geographic location (region and locality)
Cabane de Moiry, Valais, Switzerland
local environmental context
permafrost soil environment or alpine scree
negative control type
not applicable
nucleic acid amplification
PCR amplification of the full-length 16S rRNA gene from extracted soil DNA
nucleic acid extraction
DNeasy PowerSoil Pro Kits for Soil DNA Extraction
pH
4.44
pH method
2 g of frozen soil were mixed with 5 mL 0.05 M CaCl2 solution and pH was measured with a Orion Lab Star PH111 pH/mV meter (Thermo Fisher Scientific, WA, USA)
pcr primers
bacterial 16S rRNA 22f primer 5'-AGA GTT TGA TCM TGG CTC AG-3'; 1392r primer 5'-CGG TTA CCT TGT TAC GAC TT-3'
project name
Diurnal variability of soil CO₂ and CH₄ fluxes and microbial community dynamics in high-alpine soils
sample collection method
Surface soil (0–10 cm) manually collected using a sterilized shovel and transferred to sterile sampling containers.
sample name
C2_w1_16
sample storage conditions
frozen at -20°C
sample volume or weight for DNA extraction
1
scientific_name
soil metagenome
temperature
16.56
total carbon content
2.61356652
total carbon method
TOC measured on acidified soil (HCl removal of inorganic carbon) using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
total nitrogen content
33,626,253.60
total nitrogen content method
TN measured on dried and ground soil using a Thermo Finnigan Flash EA 112 CHSN elemental analyzer.
water content
9.99
water content method
Gravimetric water content was determined by drying 2 g of frozen soil at 100 °C for 24 h