Genomic DNA from Saccharina latissima strain GA_FUN2_62
DNA extracted from whole gametophyte cultures for ddRAD-seq using the Macherey Nagel Nucleospin plant II DNA plate extraction kit.
generation
gametophyte
strain
GA_FUN2_62
sample storage temperature
-80
organism
Saccharina latissima
collection date
2022-07-09
extraction_date
05/09/2024
sex
F
geographic location (longitude)
-6.958
extraction_method
NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG, Germany)
sex_determination_method
The sex of the strain was determined by PCR using the published (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1201-7#Sec28) Saccharina latissima sex-locus-specific primers Sif2057Forward (ATGCGACTCCTGTCAATTCC), Sif2057 Reverse (CTGGGTTGGGTTTTCATCAC), Sjm4021 Forward (GGCTGTTTCAAGCATGGATT) and Sjm4021 Reverse (GCGTCGTCGTTACCTGATTT) .
geographic location (latitude)
62.266
scientific_name
Saccharina latissima
collected_by
Floor Marsman, Inga Maria Mikkelsen
sample collection method
Sampling of fertile sporophyte blades. Fertile pieces of the sporophyte blade (sorus tissue) were rinsed with freshwater, wiped clean, then placed in a 50 ml Falcon tube sandwiched between two glass slides. After meio-spore release, the slides were placed in a Petri dish in Provasoli-enriched (half-strength) natural seawater to grow the released meio-spores into gametophytes. Individual gametophyte were isolated by micropipetting and grown in culture.
sample storage conditions
Frozen
ploidy
haploid
sampling_sea
north-east  Atlantic
growth condition
Sample was originally collected as a fertile sporophyte, which was then induced to sporulate and the strain was isolated as a clonal gametophyte from the resulting progeny. Gametophytes were grown as unialgal cultures (not axenic) in modified (half-strength) Provasoli-enriched seawater under red light at 13�C.
harvest_date
04/09/2025
sampling_site
Funningsfjørður A02
tissue_type
whole organism
geographic location (country and/or sea)
Faroe Islands