Metagenome-Assembled Genome: ERR9969181_bin.14_MetaWRAP_v1.3_MAG
This sample represents a Third Party Annotation (TPA) metagenome-assembled genome assembled from the metagenomic run ERR9969181 of study ERP139515.
INSDC center name
EMG
Submitter Id
ERR9969181_bin.14_MetaWRAP_v1.3_MAG
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaspades v3.15.3
binning parameters
MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
binning software
MetaWRAP v1.3
broad-scale environmental context
human digestive system
broker name
EBI-EMG
collection date
2015-07-02
completeness score
63.74
completeness software
CheckM
contamination score
0.0
environmental medium
plaque biofilm
geographic location (country and/or sea)
Australia
geographic location (latitude)
33.8688
geographic location (longitude)
151.2093
investigation type
metagenome-assembled genome
isolation_source
oral metagenome
local environmental context
oral cavity
metagenomic source
oral metagenome
project name
Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. We characterised the development of the paediatric oral resistome and investigated its role in dental caries in 221 twin children sampled over the first decade of life. From 535 oral metagenomes, we identified 309 antimicrobial resistance genes (ARGs), which significantly clustered by age, with the influence of host genetics on antimicrobial resistance increasing over time. Our results suggest the mobilisation potential of ARGs increased with age as Tn916 transposase was co-located with more species and ARGs as children grew. Dental caries compared to health was associated with a depletion of ARGs and species, which was reversed when restored. Our results highlight the dynamic nature of the paediatric oral resistome, and its potential role in transmission of AMR and dysbiosis.
sample derived from
SAMEA110373743
sample name
ERR9969181_bin.14_MetaWRAP_v1.3_MAG
scientific_name
uncultured Candidatus Saccharimonas sp.
sequencing method
Illumina NovaSeq 6000
taxonomic classification
The taxonomy of this Metagenome-Assembled Genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Candidatus Saccharibacteria;c__;o__;f__;g__Candidatus Saccharimonas;s__
taxonomic identity marker
multi-marker approach