Genomic DNA from Saccharina latissima strain GA_FWT_49
DNA extracted from whole gametophyte cultures for ddRAD-seq using the Macherey Nagel Nucleospin plant II DNA plate extraction kit.
generation
gametophyte
strain
GA_FWT_49
sample storage temperature
-80
organism
Saccharina latissima
collection date
2023-01-05
extraction_date
05/09/2024
sex
M
geographic location (longitude)
-6.950895
extraction_method
NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG, Germany)
sex_determination_method
The sex of the strain was determined by PCR using the published (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1201-7#Sec28) Saccharina latissima sex-locus-specific primers Sif2057Forward (ATGCGACTCCTGTCAATTCC), Sif2057 Reverse (CTGGGTTGGGTTTTCATCAC), Sjm4021 Forward (GGCTGTTTCAAGCATGGATT) and Sjm4021 Reverse (GCGTCGTCGTTACCTGATTT) .
geographic location (latitude)
62.246285
scientific_name
Saccharina latissima
collected_by
�lavur Gregersen
sample collection method
Sampling of fertile sporophyte blades. Fertile pieces of the sporophyte blade (sorus tissue) were rinsed with freshwater, wiped clean, then placed in a 50 ml Falcon tube sandwiched between two glass slides. After meio-spore release, the slides were placed in a Petri dish in Provasoli-enriched (half-strength) natural seawater to grow the released meio-spores into gametophytes. Individual gametophyte were isolated by micropipetting and grown in culture.
sample storage conditions
Frozen
ploidy
haploid
sampling_sea
north-east  Atlantic
growth condition
Sample was originally collected as a fertile sporophyte, which was then induced to sporulate and the strain was isolated as a clonal gametophyte from the resulting progeny. Gametophytes were grown as unialgal cultures (not axenic) in modified (half-strength) Provasoli-enriched seawater under red light at 13�C.
harvest_date
04/09/2025
sampling_site
Funningsfjørður
tissue_type
whole organism
geographic location (country and/or sea)
Faroe Islands