Microcystis-associated phycosphere MAG MAG_20, El Loto urban wetland, Chile
Metagenome-assembled genome recovered from buoyancy-enriched Microcystis-dominated colony culture, El Loto urban wetland, Los Rios Region, Chile
INSDC center name
USS
Submitter Id
ElLoto_MAG_20
assembly quality
Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs
assembly software
MEGAHIT v1.2.9
binning parameters
metawrap bin_refinement: completeness >=50%, contamination <=10% (metawrap_50_10_bins); single pooled co-assembly (all 7 read sets), no per-sample assembly/binning
binning software
metaWRAP v1.3.2 (MetaBAT2 + MaxBin2, consensus bin refinement; CONCOCT not used)
broad-scale environmental context
urban biome [ENVO:01000249]
collection date
2023-06-19
completeness score
97.39
completeness software
CheckM2 v1.0.2
contamination score
0.0
environmental medium
fresh water [ENVO:00002011]
geographic location (country and/or sea)
Chile
geographic location (latitude)
-41.254556
geographic location (longitude)
-73.009139
isolation_source
urban wetland surface water; buoyancy-enriched Microcystis-dominated colony culture (BG-11 medium)
local environmental context
wetland [ENVO:01001209]
metagenomic source
freshwater metagenome
project name
PRJEB123320
sample derived from
ERS31032356,ERS31032357,ERS31032358,ERS31032359,ERS31032360
sample name
ElLoto_MAG_20
scientific_name
uncultured bacterium
taxonomic identity marker
multi-marker approach (GTDB-Tk v2, 120 concatenated bacterial marker proteins)