Sample 24
Protocols: Blood samples were collected in 4 mL ethylene-diaminetetraacetic acid-K2 vacuum blood tubes, mixed immediately after the collection by inverting 10 times, and processed less than 8 hours after the blood withdrawal. Peripheral blood mononuclear cells (PBMCs) isolation was performed by using Ficoll (Sigma-Aldrich, St. Louis, Missouri, USA) density gradient centrifugation. Total RNA was isolated from PBMCs using the TriFast reagent (Euroclone, Pero, Milan, Italy). For assessing RNA quantity and quality we used a highly sensitive fluorescence-based quantitation system (Qubit HSRNA; Thermo Fisher Scientific, Waltham, Massachusetts, USA) and the High Sensitivity RNA ScreenTape Assay with a 4200 Tapestation System (Agilent Technologies, Santa Clara, California, USA). Libraries were prepared from 35-57 ng of total RNA, using the SMARTer Stranded RNA-Seq Kit (Clontech-Takara, Kusatsu, Shiga, Japan), following the manufacturer's instructions.
days_admission_to_blood_collection
2
clinical history
ICU_admitted
disease
coronavirus infectious disease
individual
COV_159
organism
Homo sapiens
organism
Homo sapiens
collection date
not collected
therapy
ECMO
sex
male
batch
211022
scientific_name
Homo sapiens
common name
human
organism part
blood
developmental stage
adult
isolate
not applicable
age
62
geographic location (country and/or sea)
not collected