Metagenome-assembled genome: ERP119705_co18_46
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3858116,ERR3858117 of study ERP119705.
ENA first public
2024-12-11
INSDC center name
CMR
INSDC status
public
Submitter Id
ERP119705_co18_46
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
metaSPAdes;3.15.4;default
binning parameters
coverage and kmer
binning software
MetaBAT2;2.15;default
broad-scale environmental context
aquatic
collection date
2019-01-08
completeness score
88.9
completeness software
CheckM2;1.0.2;default
contamination score
2.79
environmental medium
aquatic
geographic location (country and/or sea)
not provided
geographic location (latitude)
not provided
geographic location (longitude)
not provided
investigation type
metagenome-assembled genome
isolation_source
groundwater metagenome
local environmental context
groundwater biome
metagenomic source
groundwater metagenome
project name
The CRC AquaDiva (www.aquadiva.uni-jena.de) focuses on the important roles of water (Aqua) and biodiversity (Diva) for shaping the structure, properties, and functions of the subsurface, the part of the Earth's Critical Zone (CZ) that begins below the highest density of plant roots and extends down into the first aquifers. In this study, we generated short-read sequencing data for 32 groundwater samples from a well-characterized monitoring transect of the Hanich Critical Zone Exploratory (NW Thuringia, Germany) that encompasses 15 monitoring wells spread across a hillslope. Groundwater was collected from five wells (namely H14, H32, H41, H43, H51, H52) and was sequentially filtered through 0.2 uM, and 0.1 uM PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany). Filters were immediately frozen on dry ice and transported to a -80 C freezer. Library preparation and sequencing were performed with the help of the Core Facility DNA Sequencing at the Leibniz Institute on Aging (FLI, Jena, Germany). Briefly, libraries for all 32 samples were prepared using the NEB Next Ultra II DNA kit and multiplexed on four lanes on a NextSeq500 using 300 cycles for sequncing yielding 150 bp paired-end reads.
sample derived from
SAMEA6512636,SAMEA6512637
sample name
ERP119705_co18_46
scientific_name
uncultured Candidatus Saccharimonas sp.
sequencing method
NextSeq 500
taxonomic identity marker
multi-marker approach