R1_Bin_METABAT__289_1
Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.
ENA-FIRST-PUBLIC
2023-01-18
ENA-LAST-UPDATE
2023-01-18
External Id
SAMEA14073896
INSDC center alias
Marine College, Shandong University
INSDC center name
marine college, shandong university
INSDC first public
2023-01-18T00:18:51Z
INSDC last update
2023-01-18T00:18:51Z
INSDC status
public
Submitter Id
R1_Bin_METABAT__289_1
assembly quality
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software
MEGAHIT v1.2.9
binning parameters
anvi'o 6.2 default parameters, DASTOOL with --search-engine diamond
binning software
CONCOCT, MAXBIN2,METABAT2, refined with dastool and manually with anvi'o 6.2
collection_date
2018-10-15
completeness score
73.16
completeness software
checkM v1.1.3
contamination score
0
environment (biome)
marine biome [ENVO:00000447]
environment (feature)
microbial feature [ENVO:00002150]
environment (material)
Macroalgae
geographic location (country and/or sea)
China
geographic location (latitude)
37.562
geographic location (longitude)
122.121
investigation type
metagenome-assembled genome
isolation_source
Mariane macroalgea Grateloupia sp.
metagenomic source
marine metagenome
project name
Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments
sample derived from
ERS10980526
sample name
R1_Bin_METABAT__289_1
sequencing method
Illumina Nova-PE150
taxonomic identity marker
multi-marker approach (GTDB-tk v1.03, GTDB v95)