0273_X1_14
Protocols: C2C12 mouse myoblasts were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum and 100 μg/mL penicillin-streptomycin. Myogenic differentiation was induced by switching confluent cultures to low-glucose DMEM (1 g/L glucose, L-glutamine, 110 mg/L sodium pyruvate) supplemented with 2% horse serum and 100 μg/mL penicillin-streptomycin for 4 days. Lentiviral shRNA-mediated knockdown of Crls1, Esrrg, or Myog was performed before differentiation. Cells were treated with GSK5182 (10 μM; DMSO vehicle control at 0.1%) from day 1 to day 4 of differentiation or with N-acetyl-L-cysteine (NAC; 2 mM; ddH2O vehicle control at 1%) for the duration of differentiation, according to the experimental condition. C2C12 myotubes were homogenized and mRNA was extracted. Libraries were prepared using the Universal Plus mRNA-seq with NuQuant protocol (Tecan) as recommended by the manufacturer. Libraries were quantified with NuQuant using the CLARIOstar Plate Reader (BMG Labtech) and quality checked using a TapeStation instrument (Agilent Technologies)
library pool
A
RNA interference
Crls1 shRNA
organism
Mus musculus
organism
Mus musculus
collection date
not collected
scientific_name
Mus musculus
cell type
skeletal muscle myoblast
common name
house mouse
organism part
skeletal muscle
isolate
not applicable
cell line
C2C12
geographic location (country and/or sea)
not collected