Zea mays B73, 20 min water treatment,primary and seminal root tips (0-1mm), RNA-seq cellular transcription data, biorep 2
cultivar
B73
age
3-days after germination
dev_stage
seedling
collection_date
2025-03-20
geo_loc_name
USA:Raleigh NC
tissue
Terminal 1 mm of primary and seminal roots
biomaterial_provider
Linda Hanley-Bowdoin, 851 Main Campus Drive Partners III, rm 362 Raleigh, NC 27606
collected_by
Leigh Mickelson-Young
growth_protocol
B73 seeds were surface sterilized in in absolute ethanol for 5 min, the ethanol was poured off, and the seeds were then immediately surface sterilized in 1% NaClO; 0.05% Tween-20, rinsed and imbibed overnight with stirring and aeration. After imbibition the seeds were surface sterilized again but this time in 0.5% NaClO; 0.05% Tween-20. Seeds were germinated in 6.5x10 inch glass dishes with a clear sealing lid (Rubbermaid Brilliance) with 6 single layers of paper towels dampened with autoclaved dH2O (55 mL per dish). Seedlings were germinated at 28 C for 3 days under continuous LED light (Feit Electric OneSync LED light system) adjusted to a 3000K soft white color temperature and 300-400 lux.
sample_type
tissue sample
temp
28 C
treatment
conditioned for 20 min in water
BioSampleModel
Plant