title: Ebola virus Makona-C05 (p3 tissue culture Working Stock of PHAC isolate)
organism: Zaire ebolavirus
description: This stock was propagated from the p2 Ebola virus Makona-C05 Master stock (BioSample SAMN04488486, IRF0136_EBOV) in VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). Virus stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.