Whole Genome sequencing of Balaghat Chinnor Rice
Chinnor is a geographically indication tagged (GI) traditional rice variety of Balaghat, Madhya Pradesh, India. It has been cultivated by the traditional farmers of the Balaghat region for centuries and holds significant cultural and economic importance. It is renowned for its distinct fragrance, fine texture, and excellent cooking and eating quality that has made it a preferred choice among consumers and connoisseurs of quality rice. While, the GI-tagging of Chinnor rice protects its authenticity and market value, its genome sequencing advances genetic understanding, aiding conservation, breeding, and trait enhancement. In the present study, Genomic DNA of Chinnor rice was extracted from the leaves tissues of 20-25 days old seedlings using commercially available Xpress Plant DNA Kit (MagGenome). Quality and quantity of the extracted DNA sample was checked on NanoDrop by determining A260/280 ratio followed by agarose gel electrophoresis. The QC passed sample was processed for library preparation using Illumina TruSeq Nano DNA Library Prep Kit. Sequencing was performed on Illumina MiSeq platform using 2 x 150 bp chemistry. Processing of the sequencing data generated high quality data of 6.23 Gb covering 93.13% of the reference rice genome data.