C. elegans cross-institute experimental evolution
We evolved EVOD00 C. elegans on a novel food source, P. megaterium, across several institutes in The Netherlands and Belgium and across demographic treatments. Institutes differ randomly in the selective environment, while demographic treatments introduce varying rates of life history stochasticity. To test how each of these sources of stochasticity influence the repeatability of evolution, we sequenced pools of worms at different time points for each replicate and mapped reads to the C. elegans WBCel 235 ref and to a database of prokaryotic genomes. We use this genetic data to obtain insight into variation in C. elegans genetic diversity across institutes and treatments, variation in microbial metagenomes and to obtain allele frequencies at the beginning and end of the experiment.