Serine synthesis pathway regulates cardiac differentiation from human pluripotent stem cells [RNA-seq]
Differences of metabolism-related gene expression profiles in human pluripotent stem cells (hPSCs) and hPSC-derived cardiomyocytes (hPSC-CMs) were analyzed and successfully identified. Overall design: 253G4, 201B7, H9, NKX2-5eGFP/w cell lines were used for this experiments. hPSC-CM induction protocol was based on the modulation of the WNT signaling pathway. From Day 13 to Day 17, the cells were incubated with glucose- and glutamine-free medium supplemented with lactate for metabolic selection. hPSC-CMs obtained on Day 30 were used in the experiments.