Fagopyrum esculentum Dasha, Fagopyrum esculentum ssp. ancestrale and F2 descendants from their cross
To search for the gene which causes seed shattering in buckwheat, we needed two closely related buckwheat species with contrasting state of the trait. Fagopyrum esculentum Dasha is a common domesticated variety (non-shattering), Fagopyrum esculentum ssp. ancestrale is a wild ancestor of common buckwheat (shattering). F2 generation of their cross was obtained, the descendants were sequenced individually with low coverage and then concatenated into pools - shattering or non-shattering, correspondingly. 206 individuals form the non-shattering pool, 287 individuals form the shattering pool.