Study_OUT_fITS7_ITS4
Spikes for the mycobiome analysis were sampled 14 days after inoculation. The metagenomic sequencing analysis of the fungal hypervariable ITS2 region was conducted according to a previously described method (Wachowska, Rychcik 2023). The target region was amplified, and the library was prepared with the use of fungi-specific primers fITS7 (GTGARTCATCGAATCTTTG) and ITS4 (TCCTCCGCTTATTGATATGC), each supplemented with an overhang adapter sequence at the 5' end of each primer. A total of 280,940 fungal OTUs were obtained and assigned to 98 species.