Mechanisms and countermeasures of mammalian drug resistance acquired during long-term evolution
Drug resistance continues to impede the success of cancer treatments, creating aneed for experimental model systems that are broad, yet simple, to allow theidentification of mechanisms and novel countermeasures applicable to manycancer types. To address these needs, we investigated a set of engineeredmammalian cell lines with synthetic gene circuits integrated into their genomethat evolved resistance to Puromycin.