The changes induced by fulvestrant resistance
Gene expression in MCF7 cells and fulvestrant-resistant MCF7/182R-1 cells. Human breast cancer MCF7 cells were obtained from the NCI-60 cell lines of the NCI Developmental Therapeutics Program. The fulvestrant-resistant MCF7/182R-1 cells were obtained from the European Collection of Authenticated Cell Cultures. MCF7/182R-1 cells were maintained in DMEM described above in the presence of 100 nM ICI 182,780. RNA sequencing (RNA-seq) was outsourced to Rhelixa Inc. (Tokyo, Japan). Sequencing was performed on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads (PE150). We observed that, in 182R-1 cells, genes associated with the fatty acid elongation pathway were upregulated compared with MCF7 cells.