Genes directed by IRF7 in macropages after LPS treatment (PRJCA046271)
To analysis IRF7 binding genes, BMDMs treated with or without (control) LPS were crosslinked and lysed, and chromatin was fragmented by micrococcal nuclease. The lysates were immunoprecipitated with an anti-IRF7 antibody (Millipore Sigma, ABF130). The immunocomplexes were then purified to collect immunoprecipitated DNA which was used to generate a sequencing library and sequenced using the Hiseq 2500 system (Illumina).