RNA-seq of wild-type and ZNF217-knockout KOPN-8 cells
Here, we demonstrate that ZNF217 plays an oncogenic role in B-ALL.  To investigate the underlying mechanisms and pinpoint potential downstream targets that contribute to ZNF217's oncogenic role, we conducted high-throughput RNA-seq on both wild-type and ZNF217-knockout KOPN-8 cells. Our analysis revealed that FOS is An important target of ZNF217 in B-ALL. Overall design: Single-cloned KOPN-8 cells stably expressing Cas9 were transduced with either ZNF217 sgRNA (sgZNF217 #1 or sgZNF217 #2) or a scrambled sgRNA control. Following validation of ZNF217 knockout efficiency, cells were harvested to isolate total RNA. After that, poly(A)+ mRNAs were enriched for sequencing. For each group, the samples were prepared and sequenced in biological duplicates.