The First Complete Mitochondrial Genome of the Nemacheilus chrysolaimos (Nemacheilus chrysolaimos, Valenciennes, 1846))
The complete mitochondrial genome of Nemacheilus chrysolaimos was sequenced and assembled from a specimen collected in Temanggung, Java, Indonesia. Mitochondrial DNA was extracted from muscle tissue and sequenced using the Illumina NextSeq 2000 platform with a 300-cycle run. Genome assembly was performed using GetOrganelle (v1.7.7.1), and assembly quality was evaluated with QUAST (v5.0.2) and Qualimap (v2.2.2-dev). Annotation and visualization were conducted using MITOS (v3.4). The mitochondrial genome is circular in structure and has a total length of 16,537 bp, containing 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. This dataset provides valuable information for phylogenetic analysis and molecular identification of Nemacheilus chrysolaimos species distributed in Indonesia.