Identification of chromatin at the nuclear periphery in EMS lineage of early C. elegans embryos
We performed DNA Adenine Methyltransferase Identification (DamID) to map the contacts between the genome and the nuclear lamina in the EMS lineage of early embryos. Dam::EMR-1 and GFP::Dam fusion proteins were expressed from single-copy transgenes inserted into an intergenic region on chromosome II. To induce a short burst of transcription of the transgenes, we used the med-1 promoter, which is active in the EMS cell of 4 cell-stage embryos and repressed in the descendants of EMS. Overall design: Cell type-specific DamID in early C. elegans embryos to determine chromatin binding profiles of the nuclear envelope protein EMR-1.