Gene expression profiling of MDA-MB-231 cells incubated with therapy induced senescence secretome
TNBC, associated with poor prognosis and high tumour recurrence, are often treated with anti-mitotic drugs. However, cells may bypass treatment-induced cell death via mitotic slippage, resulting in multinucleated polyploid cells and senescence activation. Senescent cancer cells represent a population of residual disease and are highly secretory. The SASP elicited is enriched in soluble cytokines linked to tumor recurrence and distant metastasis. In contrast, sEVs derived from senescent cancer cells represent an underappreciated aspect of SASP and its mechanistic role in mediating paracrine effects remains poorly-understood. Here, we show senescent sEVs as a distinct population of SASP that could elicit anti-tumor activity. Overall design: To investigate the effect of TIS secretome, MDA-MB-231 breast cancer cells were incubated with either No CM (media only), or with CM collected from non-senescent (Cont CM) or from senescent MDA-MB-231 cells induced by 72h treatment of 100 ng/ml Nocodozole. Cells are incubated in CM for 48h prior to RNA extraction.