H3K9me3 and H4K29me3 CUT&Tag of therapy-induced senescent and diapause-like cancer cells
Analysis of the H3K9me3 and H4K20me3 differential localization over the genome of cancer cells in three conditions: proliferating cells, doxorubicin-treated cells (senescent) and INK128-treated cells (diapause-like). Two cell lines were used to perform the experiment: human melanoma SK-mel-147 cells and human NSCLC A549 cells. Overall design: Senescence was induced by treating SK-Mel-147 and A549 cells with doxorubicin (100nM). The diapause-like state was induced by treating SK-Mel-147 and A549 with INK128 (100 or 200nM). Cells were collected 7 days after the beginning of the treatment and the CUT&Tag protocol was performed. [UPDATE] Nov-20-2024: Cell line SK-Mel-103 was corrected to SK-Mel-147.