Bulk RNA-sequencing of kidneys from male C57BL/6 mice subacutely exposed to TCDD.
Bulk RNA-sequencing of 40 C57BL/6NCrl mouse-right kidney samples where males were gavaged every 4 days for 28 days with 0.03 to 30 µg/kg TCDD or sesame oil vehicle (n=5 per dose). Gavaging and tissue collection occurred between ZT0-3. Overall design: Beginning on post-natal day (PND) 30, male C57BL/6NCrl mice were repeatedly gavaged every 4 days for 28 days with some dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (0.03 - 30 µg/kg TCDD) or sesame oil vehicle (n = 5 per dose) and sacrificed on PND 58 with CO2. Gavaging and tissue collection always occurred between ZT0 and ZT3. Tissues were flash frozen in liquid nitrogen and stored at -80°C. For total RNA extraction using trizol, 100 mg was used per kidney sample. Only samples which passed Nanodrop and RIN quality control were used. 150 bp paired-end RNA reads were sequenced using a NovoSeq 6000.