Lin-sca1+cKit+ cells cultured with or without ILC2 supernatant
To investigate the effect of cytokines secreted by type 2 innate lymphoid cells (ILC2s) on hematopoietic stem cells (LSKs), we cultered LSKs with or without ILC2 conditioned supernatant (ILC2sn) We then performed gene expression profiling analysis using data obtained from RNA-seq of 3-4 samples per condition. Overall design: Comparative gene expression profiling analysis of RNA-seq data for FACS-sorted BL/6 LSKs with or without IL-33 treatment, or with ILC2sn originating from ILC2s cultured with or without IL-33