Effect of FOSL1 and HDAC2 knockdown on gene expression in cancer cell lines
We identifled FOSL1 and HDAC2 as master regulators of cancer cell metabolism. To validate their function, we knockdown FOSL1 in pancreatic adenocarcinoma cell line PANC-1 and HDAC2 in lung adenocarcinoma cell line H1299, respectively. Then RNA-seq was performed to investigate the alterations of metabolic genes. Overall design: Comparative gene expression profiling analysis of RNA-seq data for knockdown cell lines and controls. Three replicates were included for each group.