CREPE (CREate Primers and Evaluate) TAS Sequencing Data
This data was created to validate results from our novel primer design tool, CREPE. After designing primers for 1,000 randomly selected ClinVar variants, we chose three subsets of primers for targeted amplicon sequencing (TAS) validation. The No-Off dataset consisted of 95 primers with no predicted off-targets. The Low-Off dataset was a mix of primers with no predicted off-targets and what CREPE determined were low-quality off-targets. The High-Off dataset was a selection of primers with high-quality off-targets.