Ferroptosis Induced by Omega-3 Docosahexaenoic Acid (DHA) and Erastin in Control MDA-MB-231 Cells (sh-LUC) and MDA-MB-231 Cells with knockdown of IGFBP6 gene (sh-IGFBP6)
In this study, we further elucidate the mechanism of action of one of the most effective antitumor PUFAs, ?-3 docosahexaenoic acid, on MDA-MB-231 breast cancer cells, including those with IGFBP6 knockdown. Additionally, we analyzed the effect of reduced IGFBP6 gene expression on the mechanism of ferroptosis in MDA-MB-231 breast cancer cells under the influence of the canonical ferroptosis inducer erastin. Overall design: Total RNA profiles of control MDA-MB-231 cells (sh-LUC) and MDA-MB-231cells with knockdown of IGFBP6 gene (sh-IGFBP6) treated with erastin and omega-3 docosahexaenoic Acid (DHA).