S-phase checkpoint prevents leading strand degradation from strand-associated nicks at stalled replication forks
Our goal was to examine the enzymology of DNA replication during a single S-phase in the presence of replicative stress through PU-seq and GLOE-seq methodologies. Our data reveal an unexpected role of Exo1 at the leading strand and support a model of fork stabilization through prevention of unrestrained Exo1-dependent resection of leading strand-associated nicks after fork stalling.