circRNA expression profiling study by RNA-seq in colorectal cancer
To identify the most significantly differentially expressed circular RNAs (circRNAs) in colorectal cancer (CRC) tissues in terms of their expression levels .circRNA RNA-seq technology was used to screen differentially expressed circRNAs in colorectal tumor tissue samples.Sequencing was performed using a Hiseq X10 system (Illumina) in PE150 mode. For analysis of circRNA-seq, sequencing readings were mapped to the Hg19 reference genome using STAR software, and circRNA was identified using DCC tools. circRNAs that were differentially expressed in CRC were identified using the edgeR tools for screening of differentially expressed circRNA.