Minute/+ (RpS3/+) wing disc cell sequencing in Drosophila
The objective of this study was to explore genes with expression changes dependent on JNK signaling in Minute cells. GFP-positive wing pouch cells from Drosophila melanogaster larvae were isolated using a FACS Aria II cell sorter. Total RNA was then extracted from the sorted cells for RNA sequencing analysis. Samples included wild-type, RpS3/+, and RpS3/+; nub-Gal4, UAS-Puc genotypes.