Biomphalaria choanomphala transcriptome sequencing
RNA sequencing data generated from Biomphalaria choanomphala individuals originating from a University of New Mexico, USA, laboratory population established by mixing two geographically proximate populations collected by dredge from the Kenyan shores of Lake Victoria in January and July 2017. Although not intentionally inbred, the colony had been maintained under laboratory conditions for approximately four years before sequencing, likely resulting in some degree of inbreeding. For long-read transcriptome sequencing, total RNA was extracted from four individuals Bc9-Bc12 using a TRIzol chloroform protocol and pooled prior to library preparation. The pooled sample was sequenced on the PacBio Sequel SMRT Cell platform, generating Iso-Seq data for transcriptome annotation. To complement this, short-read RNA-seq data were generated from two of the individuals Bc11 and Bc12 as separate libraries and sequenced as paired-end 150 bp reads on an Illumina sequencing platform platform. These datasets were produced to support genome annotation and transcript model refinement.