Single cell RNA sequencing on PyMT mammary gland tumor cells
We labeled PyMT control cells versus PyMT-GPx2 KD with GFP in vitro and then injected into mammary fat pad of mice for incubating 45 days. We then generated single cell suspension by FACS sorting from PyMT-control, GPx2 KD. 10X Genomics was used to make cDNA library. We then sequenced the samples with Illumina high throughput sequencing. Overall design: Investigating how GPx2 loss affects mammary tumor heterogeneity and metastasis.