Multi-omics Reveals that Rumen Fluid Transplantation Affects the Immune Function of Xizang Sheep by Regulating Enterohepatic Metabolic Homeostasis
This study investigated the transcriptomic effects of rumen fluid transplantation (RFT) in Tibetan sheep to explore its impact on gut and liver function. RNA sequencing was performed on intestinal and hepatic tissues to assess gene expression changes associated with immune regulation and metabolism. RFT significantly modulated the expression of key immune and metabolic-related genes, including TLR4, NOD2, PPARA, and ABCG2. Differentially expressed genes were enriched in pathways related to immune response, metabolism, and barrier function, highlighting the role of RFT in coordinating host-microbiota interactions along the gut-liver axis. These transcriptomic data provide a molecular foundation for understanding how RFT influences ruminant health and support further multi-omics integration studies.