Identification of a new A*30:01 allele in a french  volunteer bone marrow donor
Here we describe a novel 30 :01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*03:01 ,*30:01New B*13:02 ,*47:01 C*06:02 ,*- DRB1*07:01 ,*10:01 DRB4*01:03 DQA1*01:05 ,*02:01 DQB1*02:02 ,*05:01 DPA1*01:03 ,*02:01 DPB1*01:01 ,*04:01  HLA-A*30 :01New allele differs from A*30 :01 :01 :01 by one nucleotide substitution at position  20  of exon 1  (position 20 of cDNA), where  base G is replaced by T  inducing Leu instead Arg (CGA>CTA) .