Nanopore megabarcoding of soil arthropods using COI barcode
This BioProject encompasses DNA-based megabarcoding of soil-dwelling arthropod communities, based on the individual processing of specimens followed by high-throughput sequencing. Specimens are individually extracted and PCR-amplified using indexed primers targeting a standardized barcode region (e.g., COI), enabling the traceability of sequences to individual organisms. Amplicons are subsequently pooled and sequenced using long-read technologies from Oxford Nanopore Technologies.The project is designed to generate reference datasets for biodiversity assessment, taxonomic assignment, and methodological development in large-scale arthropod barcoding.