Timosaponin AIII triggers osteosarcoma apoptosis via TLR mediated NFKB and PI3K signaling pathway inhibition
This study investigates the anti-osteosarcoma (OS) mechanism of Timosaponin A-III (Tim AT-III) using RNA sequencing of treated and untreated human OS 143B cells (three replicates per group). Transcriptomic analysis identified 576 differentially expressed genes, with key targets including TULP2, SPACA4, NCF4, CLK3, and HELLS. KEGG analysis implicated the Toll-like receptor pathway in Tim AT-III's drug-responsive inhibitory effects.