Gene expression analysis of mouse adipose tissue macrophages
We collected adipose tissue macrophages from C57Black/6 mice on postnatal day 6 (P6) and postnatal day 56 (P56) by enzyntic digestion of the inguinal fat depots and megnetic cell sorting of F4/80 antigen positive macrophages. Total RNA was isolated and used for next generation RNA sequencing. Overall design: Samples are labeled as P6 (infant) and P56 (adult) ATMs (adipose tissue macrophages).