ATAC-seq of E9.5 Neural Tube defects mouse brain and E9.5 normal mouse brains
To study the chromatin accessibility  in E9.5d mouse brains with neural tube defects or control, we performed ATAC-seq  in mouse brains. NTDs mouse model: CD-1 mice (7–8 weeks old) were provided by Beijing Vital River laboratory Animal Technology Co., Ltd, China (no: 110011200105514958, 16-25g). Female CD-1 mice were fed on low folate diet for more than 8 weeks. Sexually matured individuals were mated overnight; vaginal plug was detected at 8:00 am in the following morning and was designated as E0.5 day. NTDs mouse models were induced by intraperitoneal injection with 1.5 mg/kg of MTX (Sigma-Aldrich, USA) on E7.5. On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope. All samples with NTDs clinical manifestations were collected for experiments. Overall design: Two biological replicates are set for each group, controlA  and control B were control groups from control. And NTDsA and NTDsB were from NTDs groups.