RNA-seq of murine cytotoxic T lymphocytes in response to myosin IIA inhibition using para-nitroblebbistatin
This project explores the effects of inhibiting myosin IIA using para-nitroblebbistatin (pNB) on effector cytotoxic T lymphocyte (CTL) activation. Effector CTLs were differentiated from OTI TCR transgenic mice for 7 days in vitro, then stimulated (or not stimulated) with anti-CD3 for 1 hour, treated with either pNB or DMSO control.