zebrafish embryo for scRNA-seq and bulk RNA-seq
Hepcidin (hamp) is a key regulator for the maintenance of iron metabolism. How hamp affects the mechanism of zebrafish hematopoiesis is still largely unknown. Here, we have generated a stable hamp mutant zebrafish model by using the CRISPR/Cas9 system.We found that iron overload occurred in several tissues of zebrafish, and the hemoglobin content decreased significantly during embryonic development.Single cell profiling demonstrated that hematopoietic actors were aberrantly expressed, and hematopoietic progenitor cells appeared a group of cell clusters with relatively delayed development, accompanied by ferroptosis.