Pisum sativum Transcriptome or Gene expression
The objective of this study was to develop rapidly a comprehensive collection of SNPs evenly positioned across the pea genome and useful for breeding. In that purpose normalized sequencing strategy of 8 cDNA libraries from genotypes representative of modern pea breeding material and genotyping of a significant subset of discovered SNP allowed to generate collection of 68k cDNA contigs, to identify 35k reliable SNP markers, and to generate a high density composite genetic map based on 2070 markers including 1340 newly developed SNPs.