Oxford Nanopore Target-Indexed PCR (TIP) sequencing of organellar RNA
This BioProject contains Oxford Nanopore Target-Indexed PCR (TIP) sequencing data for targeted analysis of organellar RNA editing and transcript processing in Arabidopsis thaliana. Full-length cDNA amplicons of selected chloroplast transcripts, including ndhB and ndhD, were generated by RT-PCR, barcoded to distinguish biological replicates, pooled, and sequenced using the Oxford Nanopore platform. The dataset enables quantitative analysis of C-to-U RNA editing efficiency at defined editing sites in wild-type and genetically or environmentally perturbed conditions.