RNA-seq of Human Cortical Organoids Treated with Ciprofloxacin versus Untreated Controls
Human cortical organoids (hCOs) were employed to examine the developmental neurotoxic effects of ciprofloxacin (CPFX). Organoids were subjected to chronic exposure to low doses of CPFX for two weeks, while untreated organoids served as controls. Assessments included mitochondrial function, reactive oxygen species (ROS) production, and the expression of key neurodevelopmental regulators, including FOXG1, as well as genes associated with excitatory and inhibitory neuronal network formation. This study establishes a human-relevant model to investigate the molecular and functional consequences of CPFX exposure on cortical development.