Screening the crucial RNA among the 0, 1, 7, and 14 days during the proceeding of the C3H10 T1/2 osteogenesis differentiation.
Cultivate C3H10T1/2 cell lines using an osteogenic induction medium, extract RNA on the day of induction, the first day after induction, the seventh day after induction, and the 14th day after induction, and screen for key molecules in the osteogenic induction process through RNA seq. Overall design: 1. C3H10T1/2 cells were cultured using DMEM (10% FBS), and when the cell density reached 80, the cells were seeded onto a 6-well plate at a density of 1 * 10^6; 2. Cultivate cells using an osteogenic induction medium; 3. RNA sequencing was extracted at 0, 1, 7, and 14 days after osteogenic induction.