ABIN1 is a negative regulator of effector functions in cytotoxic T cells
The main objective of the data analysis presented here is the investigation of the role of ABIN1 in antigenic responses of primary T cells. For this purpose, T cells from ABIN1-deficient mouse strain (GTKO) and WT mice were analyzed under different activation conditions. The effect of P38 MAPK inhibitor SB203580 (p38 inhibitor/p38i) was also analyzed. The results suggest that ABIN1 is a negative regulator of TCR-mediated activation and expression of the key effector molecules in primary CD8+ T cells, at least partly via the inhibition of the p38 pathway. Overall design: Two RNAseq experiments were made. The first one compares the activation of CD8+ T cells in WT and ABIN1 GTKO mice under different conditions (anti-CD3e/CD28 beads at 1:1 or 4:1 beads-to-cell ratios or without beads). The second one compares the effect of p38 inhibitor (P38 MAPK inhibitor SB203580) on activated of CD8+ T cells in WT and ABIN1 GTKO mice. The first experiment concerns replicates 6-9, the second one replicates 17-19.