RNA-seq of human gingival fibroblasts: Control, Arecoline, and Arecoline+H1-EVs treated groups
This study aims to investigate the transcriptomic changes in human gingival fibroblasts following exposure to arecoline, a major alkaloid in areca nuts, and to evaluate the potential therapeutic effect of H1-EVs treatment. Using RNA sequencing, we compare gene expression profiles among three groups: untreated control, arecoline-treated, and arecoline-H1-EVs-treated cells. The results will help elucidate molecular mechanisms underlying arecoline-induced oral pathogenesis, such as submucous fibrosis, and assess whether ARE+EV can reverse or mitigate these effects. This work provides insights into potential therapeutic strategies for arecoline-related oral diseases.