Transcriptome profiling of engineered EPA, DHA, and wild-type Camelina sativa lines.
This project presents a time-course transcriptome data of transgenic Camelina sativa lines engineered for enhanced accumulation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The study aims to characterise gene expression dynamics of lipid-related pathways, with a particular focus on fatty acid biosynthesis and triacylglycerol metabolism, in comparison with the wild-type Camelina sativa cv. Celine. RNA sequencing was performed on developing seeds harvested at multiple developmental stages, specifically 15, 18, 20, 22, and 25 days after pollination (DAP), to capture temporal changes associated with lipid accumulation.