Hedgehog signaling is required for the maintenance of mesenchymal nephron progenitors
The goal of our study is to identify the genes regulated by Hedgehog signaling in mesenchymal nephron progenitors. Overall design: Six2GFPcre+ nephron progenitors with different Smo doses at E18.5 were isolated by FACS and subjected to RNA-seq. Smo KO = Smo c/c ; Six2GFPcre, Smo Het = Smo c/+ ; Six2GFPcre, Smo WT = Smo +/+ ; Six2GFPcre, Smo Transgenic = Rosa26 SmoM2/+ ; Smo +/+ ; Six2GFPcre