16S rDNA amplicon sequencing of fecal microbiota from DSS-induced colitis mice treated with melatonin
Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract, and accumulating evidence suggests that melatonin (MT) may exert protective effects in colitis. In this study, we investigated the impact of melatonin on gut microbiota composition in a mouse model of acute colitis induced by dextran sulfate sodium (DSS). Male C57BL/6 mice were divided into three groups: normal control (NC), DSS-treated (DSS), and DSS with melatonin intervention (DSS+MT). Fecal samples were collected when mice in the DSS group exhibited approximately 10% body weight loss. The V3-V4 hypervariable regions of the 16S rDNA gene were amplified and sequenced on an Illumina platform. The sequencing data were analyzed for microbial community composition, diversity indices, and taxonomic differences among groups. This dataset provides insights into the modulatory effects of melatonin on gut microbiota in the context of colitis and serves as a foundation for understanding the interplay between melatonin, intestinal microbiota, and colonic inflammation. The data are made publicly available to facilitate further research in microbiome-host interactions and potential therapeutic strategies for IBD.