DNA-seq of leaves samples from tomato plants and weeds collected between 1994 and 1999 across Sicily region in southern Italy showing TYLCD-associated symptoms
The aim of the project is to monitor TYLCD-associated viral species and recombinants on tomato crops and sorrounding weeds, which have a particular impact in the Mediterranean area. Total genomic DNA was extracted from pooled leaves, enriched in circular DNA, through rolling Circle Amplification and sequenced with Oxford Nanopore Technology (ONT) MinION platform.