Effect of ARL11 overexpression on gene expression of MDA-MB-231 cells during olaparib treatment
To investigate the function ARL11  in the regulation of PARPi resistance, we established MDA-MB-231 cells overexpressing ARL11. We then performed gene expression profiling analysis using data obtained from RNA-seq of ARL11 overexpression or vector control MDA-MB-231 cells treated with or without Olaparib. Overall design: 5×10^5 ARL11 overexpression or vector control MDA-MB-231 cells were plated on 10 cm dishes and cultured for 24 hours. Cells were then treated with either 40 µM DMSO or Olaparib for 3 days. Cells were lysed for RNA extraction and cDNA library construction and RNA-seq analysis;