Positional identification of a candidate gene for MALE STERILITY 2 (MS2) by linkage mapping and transcriptomic data in Cryptomeria japonica D. Don
This study aimed to identify the causal gene responsible for MS2-type male sterility in Cryptomeria japonica, a major conifer species in Japan. By integrating linkage analysis, gene expression profiling, and mutation screening, we identified a GDSL esterase/lipase gene with a missense mutation (S40F) as the most likely candidate. These findings contribute to understanding male sterility mechanisms and provide valuable genomic resources for marker-assisted selection and the development of pollen-free cultivars, helping to reduce airborne pollen and mitigate pollinosis.