Mapping chromatin remodelling in glioblastoma identifies epigenetic regulation of key molecular pathways and novel druggable targets.
Analysis of chromatin remodelling in a neoplastic stem cell context as compared to an ontogenetically related neural stem cell context reveals multifactorial epigenetic regulation of signalling pathways known to contribute to glioblastoma development. It also identifies novel epigenetically regulated druggable target genes on a patient-specific level, including SMOX and GABBR2 which could be further developed for future translational approaches to more effectively treat this neoplasm. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K4m3, H3K36me3, H3K27ac and H3K27me3 in primary human Glioblastoma Initiating Cells (GIC) and syngeneic iPSC-derived induced Neural Stem Cells (iNSC) derived from 10 glioblastoma patiens.