RNA-seq of dorsally and ventrally blastemas induced by accessory limb model (ALM) at 10 days post surgery
This BioProject provides paired-end RNA-sequencing data from the axolotl (Ambystoma mexicanum) accessory limb model (ALM) to compare dorsally induced (DorBL) and ventrally induced (VentBL) blastemas. Libraries were prepared from low-input RNA using the SMART-SeqÂ® HT Plus Kit (Takara Bio; Cat. No. R400749), yielding non-stranded (unstranded) transcriptome data. Sequencing was performed on an Illumina NovaSeq 6000 with 2Ã?150 bp paired-end reads. In total, six libraries were generated (n=3 DorBL, n=3 VentBL). The dataset was used to identify differentially expressed genes and candidate intercellular signaling molecules that may mediate dorsoventral-dependent regulation of Shh expression during limb patterning. Raw FASTQ files and minimal sample metadata are provided to facilitate reuse in regenerative biology and comparative transcriptomics.