Transcriptomics of Fallopia extract-treated radish roots
Raphanus sativus L. cv. Saxa 2 roots were treated with 10% aqueous extracts of Fallopia japonica and Fallopia ×bohemica with distilled water as control treatment. Three days after the treatment, the bottom 1 cm of the root was cut with a scalpel and immediately frozen on liquid nitrogen. The samples were homogenized using FastPrep (MP Biomedicals, ZDA) and RNA was extracted with the RNeasy Plant Mini Kit (Quiagen, ZDA). DNase- treated and cleaned samples were poly-A enriched ans paired-end sequenced using Illumina HiSeq 4000.