Epigenomic Profile of Lineage-Traced Mouse Horizontal Basal Cells Before and 24 Hours After Injury
Wild-type horizontal basal cells (HBCs) from mouse olfactory epithelium were profiled using single-cell ATAC-seq (10x Genomics v2) to identify regions of accessible chromatin in individual cells before and 24 hours after acute injury. Overall design: 24 hours after injury by intraperitoneal injection of methimazole, Krt5-CreER^T2; Rosa26-LSL-YFP/YFP lineage-traced cells were purified via fluorescence activated cell sorting (FACS). Uninjured YFP+ cells were also collected. Three biological replicates per condition (uninjured and 24 hours post-injury) were collected.