Effect of depletion of Igf2bp3 on gene expression during VSV infection in RAW 264.7 cells and mice PMs
N6-methyladenosine (m6A) modification is the most abundant RNA modification in both host mRNA and viral RNA transcripts. Dynamic regulation and recognition of m6A modification widely participate in post-transcriptional regulation of many biological processes. This project aims to define the regulation role of IGF2BP3, a m6A recognition protein, in viral infection, especially in RNA viral infection. In this study, the transcriptomes of Vesicular stomatitis virus (VSV)-infected WT/Igf2bp3-KO RAW264.7 cells and mouse peritoneal macrophages (PMs) were analyzed, suggesting the role of IGF2BP3 in the host's innate immune responses during viral infection and enhancement. Overall design: WT/Igf2bp3-KO RAW264.7 cells and PMs were infected VSV-GFP (MOI=1) for 8 hours. Total RNAs from the cells were then extracted using the Trizol reagent, following manufacturer' s instructions. Subsequently, the cDNA libraries were prepared using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (No. NR605, Vazyme) and sequenced on Novaseq 6000 (Illumina).