Gene expression profile at single cell level of murine glioblastomas treated with and without anti-CSF-1R inhibitors
Anti-CSF-1R treatment results in rapid regression of murne proneural glioblastoma models. However, the microenvironment response to treatment results in fibrosis that can promote tumor recurrence. We investigated the single-cell transcriptional profile of murine glioblastomas before and after treatment with an anti-CSF-1R inhibitor to determine the cause of fibrotic treatment response. Overall design: Gliobastomas were induced in mice using the RCAS model system at 5-weeks of age. Mice were monitored by MRI over the next 6 weeks until tumors developed that were at least 30 mm3 in volume. 3 mice were sacraficed at this time point prior to the initiation of treatment. 6 mice were treated daily with 200 mg/kg BLZ945 (Novartis), 3 were sacraficed 7-days post treatment, 3 at 14-days. All tumors were harvested from brain tissue and immediately dissociated with collagenase/dispase and gentle mechanical agitation, then single-cell barcoded cDNA libraries were created using the 10X Chormium workflow. Once all libraries were collected, samples were submited for next-generation sequencing at Genewiz.