ATF3's Dual Role in Zebrafish: Early Proliferation Enhancement and Modulates Muscle Growth while Accelerated Aging in Adulthood via Overexpression and Deficiency
Transcriptomic data for 2-month and 4-month wildtype, ATF3 overexpression and ATF3 knockout zebrafish on major organs:B: brain, E: eyes, G: gills, H: heart, M: muscle, I: intestine, S: skin. Overall design: We successfully constructed ATF3 deficient zebrafish lines and ATF3 overexpression lines using CRISPR and pTol2 separately. When zebrafish were cultured for 2 months and 4 months, were euthanized using Tricaine, dissected, and the corresponding organs (brain, eyes, gills, heart, intestine, muscle, and skin) kept in NucleoProtect RNA (TAKARA) solution. Tissue corresponding RNA was extracted and purified using NucleoSpin RNA Clean-up Kit (TAKARA) and verified the RNA quality and concentration using TapeStation RNA ScreenTape (Agilent). RNA library preparation followed the Illumina Stranded mRNA Prep protocol and checked the quality using High Sensitivity D1000 ScreenTape (Agilent). Finally, libraries were paired-end sequenced on NovaSeq X Plus sequencing platform.