Apis mellifera ligustica Royal nurse honeybees'' hypopharyngeal gland DGE project
We used gene expression analysis to examine whether queen rearing is performed by a specialized sub-caste of the nurse bees using a tag-based digital gene expression (DGE) method. From two biological replicates of hypopharyngeal gland gene expression profiles we obtained about 11.82 and 11.84 million clean tags from the NBQL group and NBWL group, respectively. And detected 158 differentially expressed genes between these two samples, with 18 genes up-regulated and 140 genes down-regulated in NBQL group compared to the NBWL group.