Assessing the role of RHIM proteins in RIPK3-transcription in MEFs
We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in death-resistant MEFs, however the molecular mechanism that governs this transcription is not well understood. Here we found that RIPK3-depedent transcription is RIPK1 and TRIF dependent in MEFs. Overall design: Ripk3-2xFv immortalized mouse embryonic fibroblasts (MEFs) lacking MLKL,  were treated with siRNA targeting ZBP1, TRIF, RIPK1 and a scramble encoded control. Activatable Ripk3-MEFs were then treated with BB homodimerizer for 4hrs. MEF RNA lysates were then submitted for bulk RNAsequencing. NOTES: AP1 = BB, qVD= pan caspase inhibitor, RXMM = ripk3-2xFVfl/fl:ubcERT2cre+MLKL-/-