Transcriptional profiling of male and female MCT-induced rats with compensated and decompensated right ventricle
We performed molecular phenotyping of RV remodeling, using transcriptome analysis of RV tissue obtained from MCT-induced rats (of both male and female animals). The clustering approach along with precise hemodynamics assessments identified “early" and "late" subgroups of decompensated state in rat RV. Further molecular characterization of these subgroups identified distinct molecular genes and pathways within different stages of RV remodeling. This study provided a resource to comprehensively compare human RV remodeling with the current animal model of PH (MCT), and led to validation of state-specific biomarkers and potential therapeutic targets for RV dysfunction. Overall design: RNA sequencing was performed for two batches of RV tissues obtained from MCT-induced rats (n=53) which were characterized as compensated RV, decompensated RV, as well as normal RV from control rats. Batch 1 include only male samples as follows: control (n=10), compensated RV (n=7), early decompensated RV (n=3), late decompensated RV (n=6). Second batch includes animals from both sexes as follows: control female RV (n=4), control male RV (n=4), compensated female RV (n=6), compensated male RV (n=5), decompensated female RV (n=3), decompensated male RV (n=5).