16S sequencing of stool samples from WT and FXYD3 cKO mice
Fresh feces were collected from FXYD3f/f and FXYD3f/f Villin-Cre littermates fed in different cages. Microbial DNA extraction, DNA quality determination, amplicon generation, PCR products mixing and purification, library preparation, and sequencing of the 16S rDNA gene were performed by Novogene Bioinformatics Technology to determine bacterial composition and diversity, using IonS5 XL 16S rDNA amplicon sequencing.