WT and heterozygous Gata2-mutant mouse HSCs
We compared the transcriptome of WT and Gata2+/- mouse fetal liver (FL) and bone marrow (BM) hematopoietic stem cells (HSCs) in various developmental stages. We defined HSCs as the lineage-cKit+Sca1+CD48-CD150+ cell population in both FL (at E14) and BM (during adulthood and aging). Additionally, we investigated the transcriptome of HSCs that are isolated from mice with WT background and transplanted with either aged-WT or aged-Gata2+/- BM cells following irradiation.