Molecular mechanisms of BCAT1 in ASK120067-resistant NSCLC cells
To investigate the mechanism of BCAT1 in mediating resistance to third-generation EGFR-TKI, we employed RNA-seq analysis to compare the transcriptome signature of ASK120067-resistant NSCLC cells with or without BCAT1 knockdown. Here, gene set enrichment (GSEA) revealed a significant decrease in the expression of genes in glycolysis pathway following small interfering RNA (siRNA)-mediated BCAT1 knockdown, which indicated the role for BCAT1 in supporting TKI resistance through upregulation of glycolysis. Overall design: Total mRNA profiles of 67R (ASK120067-resistant NCI-H1975) cells with and without BCAT1 knockdown.