PSC co-culture day6 11 samples single cell RNA-Seq
The goal of this experiment is to characterize the different cancer associated CAF subtypes that can be induced by tumour-derived signlas in pancreatic stellate cells (PSC). Co-cultures of PSC and tumour organoids were seeded in matrigel and grown for 6 days in basal media. Mono-cultures of PSC were used as controls. The PSCs were recovered by flow cytometry and subjected to single cell RNA-seq profiling via the ddSeq-Surecell system.