LjUBQ-MtDMI1 in Mtnup85
We overexpressed the Medicago common signaling pathway gene MtDMI1 or EV (empty vector) in wild-type (WT) and Mtnup85 mutant plants via hairy root transformation, and the transformed plants were grown on BNM medium for 21 days. We then collected the positive hairy roots for transcriptome sequencing.