RNA-sequencing from nasal curettes or swabs collected from young children aged 1-5
There is a paucity of data on how the mucosal immune system changes with age. Moreover, the link between nasal presence of  bacteria and viruses with the immune system in young children is  still poorly understood. To address these questions we collected nasal curettes, nasal swabs and filter papers from young children undergoing anesthesia for surgery unrelated to respiratory complaints. Microbial and host parameters were analysed from these samples, including transcriptomics from nasal curettes and swabs. Results revealed strong differences in nasal mucosal immunity in children compared with adutls, as well as clear effects of the presence of bacteria such as pneumococcus and haemophilus on the local immune system Overall design: Rna-sequencing from nasal curettes using Lexogen FWD Quantseq 75bp reads, with UMI and 15M reads per samples. No experimental groups were defined. Samples were extracted with the SPLIT RNA extraction kit.