Multi-omics Toxicological Study on Rare Earth Upconversion Nanoparticles
The total RNA of UCNPs@PEG was extracted by Trizol method after co-culture with AML12 cells for 24h, and then the mRNA was broken into fragments by breaking reagent, and the broken mRNA was used as A template to generate double-stranded cDNA by reverse transcription, and then purified Sequencing was performed. The data from sequencing is called raw data and requires filtering of the RawReads before further analysis. Trimmatic software is used for quality control and linker removal to form clean CleanReads. The processed CleanReads are aligned to the mouse reference genome using HISAT. The number of reads encoding a transcribable protein in the sample is obtained by eXpress software, normalized using estimateSizeFactors, and P values and fold changes are calculated using the R software package of DESeq. The differential genes with P<0.05 and the differential fold more than 2 were selected, and the differential genes were enriched by GO and KEGG to analyze the biological function or the influence on the signal pathway of the differential genes. The differential expression of the differential genes was displayed in the form of heat map.