RNA-seq of OsbZIP48 in Zinc deficiency
Because of its role as a key transcriptional regulator involved in the response to -Zn, the impact of OsZIP48 on global gene expression was examined. Moreover, it was assessed whether the upregulated and downregulated genes in -Zn treatment depended on functional OsZIP48. These genes likely contain direct target genes of OsZIP48. Therefore, transcriptome analysis was followed by functional validation of specific target genes. RNA-seq obtained the transcriptome of WT and osbzip48 mutant (m48-1) roots under control (+Zn) and -Zn conditions for 7 d. A large number of differentially expressed genes (DEGs) were identified between WT grown under -Zn and +Zn conditions.