CDAHFD induced NASH mouse model
Non-alcoholic steatohepatitis (NASH) is the most significant cause of chronic liver disease worldwide, with limited therapeutic options. In this experiment, a choline-deficient amino acid-defined high fat diet (CDAHFD)  were used to construct a mouse NASH model. After 16 weeks of CDAHFD  diets, liver samples were collected. We want to further confirm that the elevated EFHD2 is specifically expressed in infiltrated macrophages/monocytes in NASH. Overall design: Animal experiments were conducted using CDAHFD induced NASH models in C57BL/6J mice.Prepare mouse liver as a single-cell suspension.