Effect of 25(OH)D on the gene expression of breast cancer cell MDA-MB-453
Vitamin D as a pro-hormone is known to generate anticancer effects by various mechanisms. Vitamin D can be converted to 25-hydroxyvitamin D(25(OH)D) by the enzyme vitamin D-25-hydroxylase in the liver.25(OH)D is the precursor of 1,25-dihydroxyvitamin D(1,25(OH)2D, also called calcitriol) and can be transformed by the enzyme CYP27B1. The distribution of CYP27B1 is mainly in the kidney, but also in other tissues like breast and colon. So far, research about the anticancer effects of Vitamin D mainly chooses 1,25-dihydroxyvitamin D as the study object in vitro. However, there is increasing evidence showing that 25(OH)D also can generate anticancer effects by transforming into 1,25(OH)2D in the manner of autocrine and paracrine. Our study aims to explore the influence of 25(OH)D on breast cancer and provides the variation on the expression of RNA in the breast cancer cells cultured with 100nM 25(OH)D. Overall design: To explore the influence of 25(OH)D on breast cancer, we cultured MDA-MB-453 in the physiological concentration of 25(OH)D or its solvent for five days. The groups that MDA-MB-453 cultured in 100nM 25(OH)D or its solvent are named as experiment group(EG) or control group(CG). Each group was duplicated three times.