RNA-seq of Mucor circinelloides strain FRR5020 grown with and without calcium
The filamentous fungus Mucor circinelloides FRR5020 (Cir1 in sample name) was grown to investigate the transcriptomic response to calcium availability under reference phosphate conditions. Cultures were grown in nitrogen-limited medium containing inorganic phosphate at the reference concentration P1 (7 g/L KH2PO4 and 2 g/L Na2HPO4), with two calcium treatments: calcium present at 0.1 g/L CaCl2·2H2O (Ca1) and calcium missing (Ca0). Biomass was harvested after 5 days of cultivation (4 biological replicates per treatment), RNA was extracted, and transcriptomes were sequenced for differential expression analysis between the calcium treatments.