The role of BFGF-related transcriptional regulation  in calcification model of valve interstitial cells induced by osteoblastic medium
Purpose:To explore the  the function and underlying mechanisms of basic fibroblast growth factor (BFGF) on  calcific aortic valve disease (CAVD) a. Methods: The valvular interstitial cells were isolated from porcine aortic valve leaflets. To investigate the effect of BFGF on osteogenic differentiation of VICs, the osteogenic induced medium (OIM) and BFGF were added. RNA sequencing is used   to identify changes in gene proffles. Results:This analysis completed the full transcriptome sequencing of 9 samples, with a total of 2 differentially expressed gene groups. The number of differentially expressed genes detected was 2526, 159. 6596 lncRNAs were identified, and the number of differentially expressed lncRNAs detected was 349,100. 6179 new circRNAs were predicted, and  the number of differentially expressed circRNAs detected was 4,243. Conclusions:This study  completed for the first time to the sequencing of the full transcriptome expression of valve interstitial cells under the action of BFGF, and has proved that BFGF can inhibit the calcification process of valve interstitial cells,  our study suggests that bFGF has important value in the prevention and treatment of CAVD. Overall design: We used porcine valvular interstitial cells (PVIC) as the model. We used OIM differentiation medium to induce calcification as well as bFGF treatment to inhibit the calcification for 3 days. The PVICs expression profiles of mRNA and its related transcriptional regulation were investigated by transcriptome sequencing.