EP300 genomic binding analysis in MCF7 cells with E-cadherin inhibition using ChIP-seq
This experiment examines the genomic binding of EP300 in MCF7 breast cancer cells after E-cadherin inhibition. Using ChIP-seq, we identified EP300 binding sites in control (cAb-MCF7) and E-cadherin-inhibited (nAb-MCF7) cells. The goal was to understand how EP300's chromatin binding is altered and its role in regulating genes involved in epithelial-mesenchymal transition (EMT) and pluripotency. The workflow included ChIP with EP300 antibodies, sequencing, and data analysis to identify differential binding regions associated with key biological processes.