Transcriptome analysis of bulbil development in Dioscorea polystachya cv. Tiegun
Chinese yam (Dioscorea polystachya Turcz. cv. Tiegun) plants were cultivated in April 2024 at the experimental field of the College of Life Sciences, Henan Normal University, Xinxiang, China (35.19 N, 113.52 E). Bulbil samples were collected at three representative developmental stages: 70 days after planting (DAP; T0), 115 DAP (T3), and 180 DAP (T6). At each stage, bulbils were harvested, immediately cut into small pieces, frozen in liquid nitrogen, and stored in an ultra-low-temperature freezer until further RNA extraction and transcriptome sequencing.