RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colonoids (in vitro; 0, 0.1, 10 µM; 24, 48, 72 h), with raw paired-end FASTQ files
Doxorubicin (DOX) is a widely used chemotherapeutic agent that can cause off-target gastrointestinal (GI) toxicity. This experiment profiles DOX-induced transcriptomic responses by RNA-seq in mouse colonoids (in vitro) across dose and time. Mouse colonoids were sampled at 24, 48 and 72 h after exposure to 0 (vehicle control), 0.1 or 10 µM DOX. The resulting RNA-seq data support analysis of GI toxicity mechanisms in an in vitro mouse intestinal model. A matched mouse colon (in vivo) RNA-seq dataset is deposited separately and will be cross-referenced via accession in the manuscript and/or the related accession field.