IGF2BP3 Regulates Expression and Alternative Splicing of Genes associated with the Progression of Gastric Cancer in AGS cells
Gastric cancer has become the fifth largest malignant tumor in the world, with a mortality rate ranking fourth. IGF2BP3, as a multifunctional RNA binding protein, is involved in regulating alternative splicing and m6A modification. Research has shown that IGF2BP3 plays a carcinogenic role in the development of gastric tumors. However, there is currently relatively little research on the impact of IGF2BP3 as an RNA binding protein (RBP) on alternative splicing in gastric cancer cells. The specific function of IGF2BP3 as an RBP and its molecular mechanisms involved in the development and progression of gastric cancer are still unclear. The aim of this study is to explore the mechanism by which IGF2BP3 affects gene expression and alternative splicing by binding to mRNA, and thus plays a role in the development of gastric cancer cells. We overexpressed IGF2BP3 in gastric cancer cells (AGS) and obtained transcriptome data (RNA seq) on the effects of IGF2BP3 using high-throughput transcriptome sequencing. We then analyzed potential targets in AGS cells where transcription and alternative splicing levels are regulated by IGF2BP3, and explored the molecular mechanisms by which IGF2BP3 affects gene expression and alternative splicing in AGS cells. Overall design: By overexpressing IGF2BP3  in  AGS cell, whole transcriptome sequencing (RNA-seq) analysis helped us systematically investigated the down stream targets of IGF2BP3 , including differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs).