Studying the microbiota in bladder cancer
Bladder cancer(BC) is the ninth most frequent malignant disease, with more than 160,000 deaths per year reported globally. Research on microbiota in tissues of BC is scars. We investigated the interatumoral microbiota and urine samples in BC tissue biopsies from 20 patients diagnosed with BC, non-muscle invasive bladder cancer (NMIBC) T1 (n=10) and Ta(n=10). Urine samples representing the tumors samples from the 20 patients(n=10) were collected. The microbial DNA was isolated from tissue and urine samples. Next-generation sequencing,and16S rRNA gene from extracted gDNA was used. Library Preparation and nanopore sequencing were done through the amplification of the 16S gene.