16s rRNA Amplicon Sequencing of Original and Bioaugmented Groundwater Sample with DD4
Study investigated the efficiency of 1,4-dioxane degradation by DD4 in two groundwater sample in landfill leachate site located in Pennsylvania, USA. The site contaminated 1,4-dioxane concentration is as low as tens of ppb, which is completely degraded by inoculated strain DD4 using propane and 1-propanol as auxiliary substrate. For community analysis v3-v4 region of bacteria was amplified using 338F and 806R primers. The resulting amplicons were sequenced using Illumina MiSeq sequencing technology. Microbial community diversity was analyzed before and after augmentation with DD4 in both samples MW-A and MW-B with different dioxane(onsite) concentration.