T cell receptor-engineered T cells targeting the TP53R248Q neoantigen
This dataset provides single-cell RNA sequencing (scRNA-seq) and paired T-cell receptor (TCR) sequencing data for antigen-specific CD8+ T cells, generated using the 10x Genomics Chromium platform. CD8+/Tetramer+ cells were FACS-sorted with high viability (>98%) and processed according to the manufacturer's protocol for 5' gene expression and V(D)J enrichment. Rigorous quality control was performed using Bioconductor packages (DropletUtils, scater, scran), filtering out low-quality cells, genes detected in fewer than three cells, and cells with high mitochondrial content. TCR sequences were assembled and analyzed using Cell Ranger and the Loupe VDJ Browser, enabling the identification of clonotypes based on paired alpha and beta chains. The dataset captures the full-length, paired TCR sequences alongside the transcriptional profile of individual antigen-specific T cells. It serves as a resource for studying clonal expansion, transcriptional states, and functional heterogeneity within antigen-responsive T cell populations, supporting research in immunology, vaccine development, and T cell engineering.