Directional endothelial communication by polarized extracellular vesicle release
EVs were isolated from primary human aortic endothelial cells (ECs) (+/- IL-1b activation) grown on transwells, quantified, and analysed by miRNA transcriptomics. Overall design: EV-microRNA profiles were determined using HTG Molecular Diagnostic EdgeSeq technology (HTG Molecular Diagnostics, Inc, Tucson, AZ, USA).