RNA-seq for DIMT1 wild-type and E85A mutant overexpression in DIMT1 heterozygous knockout HEK cells
Dimethyladenosine transferase 1 (DIMT1), an 18S rRNA N6,6-dimethyladenosine (m26,6A) methyltransferase, participates in ribosome assembly. However, whether the catalytic activity is of importance in ribosome assembly and other cellular processes are not fully understood. To better understand the role of DIMT1 catalysis in gene regulation and cellular function, we performed RNA-seq with purified poly(A)-RNA from DIMT1+/- + wild type cells and +E85A variant cells. Overall design: RNA-seq in DIMT1 wild-type and E85A variant overexpression cells