N6-methyladenosine (m6A) methylated RNA immunoprecipatation and sequencing in wildtype and Mettl5-/- embryonic stem cells.
We report the application of methylated RNA immunoprecipatation and sequencing technology for high-throughput profiling of m6A modifications in embryonic stem cells (ESCs). By using proteinA/G and m6A antibody, we obtained immunoprecipitated RNA of mouse ESCs. RNAs can be read in a single nucleotide accuracy analysis. Overall design: RNA m6A profiles of wild type (WT) and Mettl5-/- mESCs