Capturing totipotency in human cells through spliceosomal repression [scRNA-seq]
Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Overall design: teratomas derived from hESCs and hTBLCs are dissociated and performed single cell RNA sequencing. hTBLC and naïve hESCs cultured in E8 medium are collected at different time points and performed single cell RNA sequencing.