CUT&RUN data of Nanog and Sox2 in mouse embryonic stem cells treated with auxin for CHD4 depletion against untreated control
This study investigates the role of the chromatin remodeller CHD4 in regulating transcription factor binging using CUT&RUN. CHD4 was tagged with a mini-AID degron to enable auxin-inducible degradation. CUT&RUN for Nanog and Sox2 was performed at multiple time-points (30 minutes, 1 hour, and 4 hours) following auxin treatment to capture changes in their binding upon CHD4 depletion. For each time point, two biological replicates were processed, each with two technical replicates. This dataset aims to characterise the immediate effects of CHD4 loss on TF binding.