RNA seq of breast cancer cells treated with different concentration gradients of Thapsigargin (TG), Tunicamycin (TM), and FCCP.
To investigate the effects of TG, TM, and FCCP treatments with different concentration gradients on breast cancer cells. Overall design: MDA cells were treated with FCCP (0, 0.5, 1, 2 µM), FCCP (5 µM) + MG132 (10 µM), FCCP (5 µM) + 3-MA (5 µM), TG (0, 1.25, 2.5, 5 µM), TG (2 µM) + MG132 (10 µM), TG (2 µM) + ISRIB (0.5 µM), TG (2 µM) + melatonin (10 µM), TG (2 µM) + 4µ8c (20 µM), TM (0, 0.5, 1, 2 µM), TM (2 µM) + MG132 (10 µM), TM (2 µM) + ISRIB (0.5 µM), TM (2 µM) + melatonin (10 µM), TM (2 µM) + 4µ8c (20 µM) for 24 hours. Two biological replicates were prepared for each condition.