Optimization of RNP-CRISPR for High-Efficiency Gene Editing in Mouse Intestinal Organoids
This project presents the Sanger sequencing data of the edited Apc and Kiss1 loci in mouse intestinal organoids following CRISPR/Cas9 RNP electroporation. Mouse intestinal organoids serve as valuable models for studying intestinal development, homeostasis, and diseases like colorectal cancer. The CRISPR/Cas9 ribonucleoprotein (RNP) electroporation technique was optimized to achieve high-efficiency gene editing with minimal cellular toxicity, enabling precise genetic modifications without compromising the organoids' functionality. The Sanger sequencing data provided here offers insights into the accuracy and efficiency of the gene editing process at the Apc and Kiss1 sites, facilitating further research into the mechanisms of intestinal diseases and the development of potential therapeutic strategies.