Type I interferon protects against bone loss in periodontitis by mitigating an interleukin (IL)-17-neutrophil axis [scRNA-seq]
We determined the early inflammatory infiltrates on day 3 after ligature placement, a stage prior to noticeable bone loss, by performing single -cell RNA sequencing (scRNA -seq) Overall design: We prepared the gingival single -cell suspension pooled from two ligated Ifnar1 -/- mice and two ligated WT mice separately and sorted out live CD45 + immune cells, on which scRNA -seq was perform