Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI
Dysregulated endometrial receptivity is a well-established critical factor that contributes to recurrent implantation failure (RIF). Decidualization of stromal cells and differentiation of epithelial cells in the endometrium are crucial processes for achieving endometrial receptivity. Menin, the unique subunit of the H3K4 methyltransferase complex, exhibits cell-specific effects on gene expression through chromatin modification by histone 3 lysine 4 trimethylation (H3K4me3). We have previously reported the significant role of Menin-regulated modifications in H3K4me3 in the maintenance of early pregnancy in mice. However, the physiological function of Menin and its interaction with H3K4me3 in regulating human endometrial receptivity remain poorly understood. Here, we report that Menin expression is reduced in the endometrial stroma of RIF patients. Stromal Menin deficiency not only impaired the decidualization of stromal cells but also negatively impacted the differentiation of epithelial cells through HAND2-FGFs-FGFR axis. Transcriptome analysis revealed that MEN1 knockdown in stromal cells induced the aberrant activation of the WNT signaling pathway, and in vivo experiments showed it was associated with a significant reduction in the weight of implantation sites. Mechanistically, Menin deficiency suppressed the expression of SFRP2 and DKK1, which are negative regulators of the WNT signaling pathway, through H3K4me3. In summary, our study identified Menin as a critical regulator of endometrium receptivity, advanced our understanding of its molecular mechanisms, and highlighted its potential role in the pathogenesis of RIF.