RNA-seq analysis of myeloid cells in dLNs post-administration of mRNA vaccine
To investigate transcriptional signatures of myeloid cells in dLNs post-administration of mRNA vaccine, we collected dLNs from mice 24 h post-vaccination, sorted six types of myeloid subsets using FACS, and performed RNA-seq analysis. RNA-seq data showed increased expression of markers related to proinflammation and antigen presentation throughout myeloid subsets in dLNs post-administration of the mRNA vaccine. Overall design: To investigate transcriptional signatures of myeloid cells in dLNs post-administration of mRNA vaccine, we collected dLNs from mice 24 h post-vaccination, sorted six types of myeloid subsets using FACS, and performed RNA-seq analysis.