YAP-Driven Malignant Reprogramming of Oral Epithelial Stem Cells, 3' single cell RNA sequencing
Single cell RNA sequencing of whole tongue epithelia from control mice or transgenic mice expressing HPV16E6-E7 and/or YAP1S127A Overall design: Fifteen days after transgene induction, cells were isolated from whole tongue epithelia, and subjected to oligo-dT-based mRNA selection, cDNA library preparation, and sequencing on Illumina NovaSeq6000.