Transcriptomic profiling of bovine circulating neutrophils during in vitro lipopolysaccharide challenge across different energetic states
This study investigated the impact of different energetic conditions on the transcriptomic landscape of polymorphonuclear neutrophils (PMNs) subjected to in vitro lipopolysaccharide (LPS) challenge. Circulating PMNs isolated from four pubertal nulliparous heifers (four biological replicates) were suspended in culture medium supplemented with glucose (1.5 mM or 3 mM to mimic hypo- and normo-glycemia) and 1 mM non-esterified fatty acids (NEFA). Control groups received no glucose or NEFA. After 3 hours of incubation, PMNs were challenged with 50 ug/mL LPS for 1 hour to simulate infection. Total RNA was extracted post-challenge and sequenced using an Illumina platform.