Effects of ash-2, hsf-1, or ash-2 + hsf-1 RNAi on gene expression in Day 1 adult Q40::YFP C. elegans
To determine how gene expression changes in long-lived ash-2-deficient worms expressing the amyloidogenic Q40::YFP, and to assess how simultaneous depletion of hsf-1 effects these transcriptomic changes. Overall design: Q40::YFP C. elegans were synchronized by timed egg lay on plates containing L4440 (control), ash-2, hsf-1, or ash-2 + hsf-1 RNAi E. coli HT115 (DE3) bacteria. Animals were then aged at 20°C for 96 h, then were washed from plates with M9, pelleted, and flash frozen in liquid nitrogen. Parents of animals destined for ash-2 or ash-2 + hsf-1 RNAi plates were also maintained on ash-2 RNAi since egg.