Analysis of the transcriptional impact of WDR4 and/or METTL1 knockdown in glioma cells
m7G tRNA modifications play important role in maintaining translational fidelity. METTL1-WDR4 complex is responsible for m7G sythesis in the tRNA. m7G was shown to be important for oncogensis of many cancer types, however, its role in glioma was not explored. To evaluate whether m7G can be a target for glioma therapy, we knocked down METTL1, WDR4, or both using shRNA lentiviral transfection in U87 malignant glioma cells. Here, we analyzed the transcriptional impact of such knockdown of each knockdown.