Transcriptome analysis of NAFLD mice
There were 12 longRNA-seq samples, including 4 in the traditional Chinese medicine group, 4 in the blank control group, and 4 in the non-alcoholic fatty liver disease (NAFLD) group. There were also 12 smallRNA-seq samples, with 4 in the traditional Chinese medicine group, 4 in the blank control group, and 4 in the NAFLD group. The reference species was Mus musculus, and the reference genome version was GRCm39. /nLongRNA-seq sequencing was performed on mouse liver tissue (using an rRNA-depleted strand-specific method to construct sequencing libraries, simultaneously sequencing messenger RNA (mRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA)). Additionally, small RNA-seq sequencing experiments were conducted (utilizing a high-throughput sequencing platform to sequence enriched small RNA fragments of 18-32nt).