Effect of essential oil- and iodine treatments on the microbiota of the brown alga Ectocarpus siliculosus.
In this study we evaluated the impact of essential oil, povidone-iodine, and antibiotic treatments on the Ectocarpus microbiota. We performed 16S Metabarcoding of the V3-V4 region with the standard Illumina protocol for metabarcoding (Q5® High-Fidelity PCR Kit, AMPure XP for PCR Purification Kit and Nextera XT DNA Library Preparation Kit), and the sequences were obtained with an Illumina MiSeq sequencer using a MiSeq v3 kit (2x300bp, paired-end).