Bulk RNA seq of in vitro co- culture of distinct subtypes of oral cancer associated primary fibroblasts and oral cancer cell line
Heterogeneity in oral cancer associated fibroblasts (CAFs)in tumor microenvironment modulate plasticity of malignant epithelial cells to drive tumorigenesis, ultimately leading to poorer clinical outcome. Myofibroblastically differentiated CAFs induce stochastic plasticity in oral cancer cells. We co-cultured non-myofibroblasts and myofibroblasts with oral cancer cell lines to understand distinct CAF driven cancer cell transcriptional reprogramming. Overall design: Gene expression profiling analysis of RNA seq data from in-vitro 48 hrs co-culture of untreated(C1 CAF) and TGFb treated primary CAFs (myofibroblasts/C2 CAF) with oral cancer cell line SCC070. CAFs and SCC070 cells were sorted  by flow cytometry using CD90 marker before library preparation.