Colorado potato beetle metabolic detox transcripts
This study's goal was to quantify insecticide susceptibility and resistance in adult Colorado potato beetle (Leptinotarsa decemlineata) populations from multiple U.S. locations. Adult beetles were exposed to a range of concentrations of two insecticides, imidacloprid and isocycloseram, and mortality and incapacitation were recorded at 48 and 96 hours post-exposure. These experiments were conducted to estimate dose-response relationships, lethal dose metrics, and resistance ratios within and across populations. In addition, targeted transcript expression data were generated for a select set of 299 genes previously associated with metabolic detoxification and insecticide resistance. Transcript expression was quantified for selected populations and treatments to evaluate molecular responses to insecticide exposure and to support analyses linking phenotypic resistance patterns with detoxification-related gene expression.