Universal Metabarcodes (16S & 18S) from Microbial Antarctic Time Series (2013-2019)
This metabarcoding dataset is derived from PCR amplification of marine plankton-derived DNA from the Microbial Antarctic Time Series (2013-2019). Metabarcoding reads were generated using a single primer set that simultaneously amplifies both 16S and 18S genes, though users should note that specific bioinformatic procedures are required to recover and analyze 18S sequences (see: https://github.com/jcmcnch/eASV-pipeline-for-515Y-926R).This dataset has produced metabarcoding data from the universal primer set (Parada et al., 2016, doi:10.1111/1462-2920.13023) on unfractionated samples (> 0.2 um). This primer set perfectly matches the rRNA of most surface ocean organisms, including eukaryotic and metazoan 18S (McNichol et al., 2021, doi:10.1128/msystems. 00565-21). As a result, the sequences here represent a full-community profile of each water sample with the same denominator and the same primer set (Yeh et al., 2021, doi.org/10.1111/1462-2920.15553).Notes for data users:-Only limited environmental covariate data has been uploaded with the raw sequences.-Bioinformatic intermediates, and scripts are stored at OSF in a single umbrella repository (https://osf.io/57dpa/). This is a useful place for those who might wish to analyze only a subset of our data (e.g. 18S or 16S only) or who wish to understand the bioinformatic processing in greater detail.-These data also use internal standards, meaning that the final dataset can be normalized to gene copies/L, these internal standards are notated in SILVA as species "Thermus_thermophilus", "Blautia_producta", and "Deinococcus_radiodurans". The script used to normalize the data can be found at (https://github.com/Nwilliams96/Project-3-515Y-926R-internal-standards)PCR amplification and sequencing:5' master mix was used for DNA amplification with the 515Y (59-GTGYCAGCMGCCGCGGTAA) and 926R (59-CCGYCAATTYMTTTRAGTTT) primers with Illumina adapters and barcodes pre-ligated (as noted here: dx.doi.org/10.17504/protocols.io.vb7e2rn). Sequencing was done using the Illumina HiSeq RapidRun2 x 250 technology at Tufts Medical School.