A Unique Type V CRISPR-Cas System Encoded by a Group of Thermus Viruses
In this work, we investigated the transcriptional landscape of Thermus thermophilus cells infected with the previously isolated Lalka27a phage. The analysis revealed temporal patterns of phage gene expression. One of the highly transcribed loci encodes a unique Type V CRISPR-Cas system that is active in T. thermophilus cells. Using RNA-seq data, we determined the structures of the guide RNAs and mapped phage promoters.