Circadian transcriptome analysis in mouse cultured cortical astrocytes
To understand how the circadian clock regulates astrocyte physiology, we conducted a circadian transcriptome analysis in cultured mouse cortical astrocytes Overall design: After synchronizing the circadian clock of cultured astrocytes by serum shock, cells were collected at intervals of 4 hours, starting from 12hrs post sync to 56hrs post sync. Subsequently, collected cells were subjected to RNA sequencing.