La Selva Biological Station (Costa Rica) Neotropical lowland rainforest soil DNA metabarcoding of the fungal ITS2
A total of 94 soil samples were collected in the lowland rainforest of the La Selva Biological Station (Costa Rica) in October 2015. All samples were collected and prepared following the protocol of Mahé et al. (2017). General primers (Toju, Tanabe, Yamamoto, & Sato, 2012) were used to amplify the ITS2 region of the Internal Transcribed Spacer. The amplified fragments were sequenced on an Illumina MiSeq v3.Mahé, F., de Vargas, C., Bass, D., Czech, L., Stamatakis, A., Lara, E., … Dunthorn, M. (2017). Parasites dominate hyperdiverse soil protist communities in Neotropical rainforests. Nature Ecology & Evolution, 1, 0091. doi:10.1038/s41559-017-0091Toju, H., Tanabe, A. S., Yamamoto, S., & Sato, H. (2012). High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples. PLOS ONE, 7(7), e40863. doi:10.1371/journal.pone.0040863