Effects of PAK4 knockdown on gene expression in glioblastoma-associated endothelial cells
PAK4 was knocked down in human glioblastoma (GBM)-associated endothelial cells (ECs) using CRISPR-Cas9/gRNA. RNA was isolated and analyzed by RNAseq. The effects of PAK4 knockout on gene expression were determined. Overall design: ECs were isolated from GBM tumors and transduced with the lentivirus expressing PAK4 sgRNA or control sgRNA, followed by cell selection by incubation with medium containing 2 µg/ml puromycin. RNA was isolated and analyzed by RNAseq.