RNA-seq of Zrsr1-2WT and Zrsr1-2 mutant embryos
Zrsr1 and Zrsr2 are two paralogous genes, whose products play a key role in minor splicing. To investigate the effects of minor splicing during early embryo development, embryos from Zrsr2 mutant females and Zrsr1 mutant males were produced, showing an impaired development during zygotic gene activation at the 2-cell stage. RNA-seq of these embryos was performed to check the complementation of these two genes during this stage.