Transcriptome profiling of Actr3 KO mIMCD3 cells
Actr3 WT (control) and Actr3 KO mIMCD3 cells were generated using CRISPR/Cas9 and independent gRNAs for control and KO cell lines. Single cells clones (Ctrl.-1, Crtl.-2, KO-1, KO-2) were selected and validated by western blotting confirming loss of Actr3 in KO cell lines on protein level. Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis of independent replicates per cell line was performed. Overall design: mRNA sequencing analysis of control and Actr3 KO mIMCD3 cell lines