Ocean Metaproteome Intercomparison Consortium: Metagenome Sequencing Reads for Proteome Informatics & Data Analysis Intercomparison
Ocean metagenome sequencing reads used for metaproteome intercomparisons of proteome informatics and data analysis methods. Short- and long-read metagenome reads deposited here originated from Bermuda Atlantic Time Series (BATS) expedition 348. Environmental DNA was extracted from filters and sequenced via Ion Torrent and Oxford Nanopore technologies. Following de novo metagenome assembly, predicted protein-coding open reading frames (ORFs) were translated in silico to yield ocean metaproteome reference sequences. Multiple laboratories used the metaproteome reference sequences for peptide-spectrum matching (PTM) based identification strategies from nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS) data. Intercomparison of data analyses among laboratories will be reported in forthcoming manuscript. The primary LC-MS/MS data used among comparisons is publically-available via the ProteomeXchange Consortium's PRoteomics IDEntification (PRIDE) database.