Single cell RNA sequencing data from a complete human fetal embryo at carnegie stage 17 (sample ID: F158 for Haniffa Lab; 16099 for HDBR)
Investigating the blood, immune and stromal cells present in a human fetal embryo in a world first single cell transcriptomic atlas. The embryo was dissected into 12 coronal (3x4) sections of ~2mm^3 each, live single cells sorted, with cell suspension then undergoing 10x chromium 5 prime scRNA-seq. The broad section descriptors are as follows: section one (lower vertebrae), two (lower vertebrae and yolk sac stalk), three (mid-low vertebrae), four (liver), five (mid vertebrae), six (internal organs), seven (brain/hear/eyes), eight (upper vertebrae), nine (internal organs), ten (brain/heart/eyes), eleven (brain/stroma), twelve (brain/stroma). A matched accession contains the yolk sac and yolk sac stalk data from this embryo. Termination procedure for this embryo was medical. The F158_[features...barcodes...matrix].[tsv...mtx].gz files attached to this accession represent raw count data from all the 10x lanes in this accession combined, and as output from CellRanger filtered matrices (CellRanger version 6.0.1 using human reference genome GRCh38-2020-A).