miRNA Expression Profiles in Burkitt and Hodgkin lymphoma cell lines upon Ionizing Radiation Exposure
The aim of this study was to identify miRNAs that undergo regulated biogenesis in response to ionizing radiation in B-lymphoma cells. The dataset includes miRNA sequencing data from three Burkitt lymphoma cell lines (ST486, CA46, DG75) and three Hodgkin lymphoma cell lines (L1236, L428, KMH2). Cells were exposed to 4 Gy dose of ionizing radiation and compared to non-irradiated control cells. Single-end RNA sequencing was performed using the Illumina NextSeq500, platform, generating high-quality reads for the analysis of miRNA expression levels and gene expression profiles related to miRNA biogenesis. The raw data analysis followed a multi-step approach. Adapter sequences were removed with Cutadapt-1.11. The reads were then aligned to a modified miRBase (v22.1) using Bowtie-1.2.2, customized to fit miRge2 specifications. An iterative alignment strategy was employed: the reads were initially mapped to mature miRNA sequences from miRBase v22.1, and those that remained unaligned were subsequently mapped to miRNA hairpin sequences, non-coding RNAs, and the Ensembl cDNA database. Any unaligned reads were re-aligned to mature miRNA sequences under more relaxed conditions.