Long-Read Amplicon Sequencing of bovine NANOS3
PacBio long-read amplicon sequencing of bovine NANOS3. This study employed cytoplasmic microinjection of the CRISPR/Cas9 system in in vitro-produced bovine zygotes to generate NANOS3 knockout bovine samples. Long-read amplicon sequencing was used to evaluate the resulting mutations present in each sample.