Identification of a new A*23:01 in a french  volunteer bone marrow donor
Here we describe a novel A*23 :01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*23:01 New A*68:02 B*18:01 ,*44:03 C*04:01 ,*05:01 DRB1*04:05 ,*07:01 DRB4*01:01 DRB4*01:03 DQA1*02:01 ,*03:03 DQB1*02:02 ,*03:02 DPA1*01:03, *- DPB1*04:01 DPB1*104:01  HLA-A*23 :01New allele differs from A*23 :01 :01 :03 by one nucleotide substitution at position 30   of exon 5  (position 925 of cDNA), where  base G is replaced by A without aminoacide change (GTG>ATG) .