Bulk RNA sequencing of murine microglia cell line
Microglia, brain innate immune cells, participate in the spread of inflammatory signals and aggregated proteins through secretion of extracellular vesicles (EVs). Selenoprotein P (Sepp1) is a potential regulator of microglial EV secretion. Here we investigate the effect of Sepp1 silencing on microglial transcriptomics to elucidate the Sepp1 regulatory mechanism of EV secretion. In this project we performed a bulk RNA sequencing of BV-2 microglia cell line stable expressing CD63-tdTomato and three different clones of TurboGFP-shRNA targeting Sepp1 or scrambled sequence (one single clone).