Mitochondrial transfer mediates endothelial cell engraftment through mitophagy
?One of the downstream effects of mitophagy is the transient activation of mitochondrial biogenesis and, in turn, enhanced mitochondrial respiration. ?To gain insights into the transcriptional changes produced in ECs by incorporating exogenous mitochondria through either co-culture with MSCs (mitoT-ECs) or artificial transplantation (mitoAT-ECs), we performed RNA-seq analysis. Overall design: Comparison the transcriptomic difference among three groups of ECs corresponding to (1) ECs that received exogenous mitochondria by artificial transplantation (mitoAT-ECs), (2) ECs that received exogenous mitochondria (from MSCs) by coculture with MSCs (mitoT-ECs), and (3) ECs that were co-cultured with MSCs but did not receive exogenous mitochondria (unprimed-ECs), served as control. [UPDATE] 05-09-2024: The Sample titles were updated. The old titles are in the Sample description field.