RNA-seq data for APOE2/3/4 treatment on HMC3
WT and LilrB3-null HMC3 cells were treated by different isoforms of APOE proteins. Each of the three full-length APOE proteins purified from the same batch under identical conditions were concentrated and diluted to 0.24 mg/mL (about 7 micromolar) using PBS. The APOE proteins and the PBS control were 10-fold diluted using the culture medium to make the APOE treatment solution and the PBS control treatment solution. The final APOE concentration was 24 microgram/mL (about 0.7 micromolar). Each treatment by APOE or PBS control was applied to three culture plates (as three replicates). 36 hours after the treatment, the cells were harvested, and RNA were purified. The RNA was subjected to RNA-Seq analysis using MGI-2000 system (Beijing Genomics Institute (BGI), China).