Lysine-restricted diet reshapes gut microbes to ease obesity-related anxiety
Total RNA from cortical tissues was extracted and purified using TRIzol reagent (Invitrogen, CA, USA) and DNase I. High-quality RNA samples were used for subsequent analysis. After purification and reverse transcription, RNA transcriptome libraries were constructed using the TruseqTM RNA Sample Preparation Kit and sequenced on the IllumimaNovaSeq6000 platform. In bioinformatics analysis, RSEM was used to quantitatively measure gene abundance. Two groups of differentially expressed genes (DEGs) were identified using DESeq2 .