Overexpression of miR-200c/141 and miR-200b/200a/429 in MDA-MB-436 cells
We previously showed that overexpression of the miR-200c/141 cluster in the TNBC cell line MDA-MB-231 significantly reduced proliferation and invasion in vitro and tumor growth and metastasis in vivo. To confirm the effects of miR-200s in another TNBC cell line, MDA-MB-436 cells were created to overexpress both the miR-200c/141 cluster and the miR-200b/200a/429 cluster. Overall design: Both the miR-200c/141 cluster and the miR-200b/200a/429 cluster were overxpressed in MDA-MB-436 cells using lentiviral vectors to create MDA-436-200f cells. A control lentiviral vector was used to create MDA-436EV cells