A cellular and molecular spatial atlas of dystrophic muscle
Asynchronous skeletal muscle degeneration/regeneration is a hallmark feature of Duchenne muscular dystrophy (DMD); however, traditional -omics technologies that lack spatial context make it difficult to study the biological mechanisms of how asynchronous regeneration contributes to disease progression. Here, using the severely dystrophic D2-mdx mouse model, we generated a high-resolution cellular and molecular spatial atlas of dystrophic muscle by integrating spatial transcriptomics and single-cell RNAseq datasets. Overall design: Gene expression profiling analysis of spatial transcriptome data taken from wildtype (DBA2/J) and dystrophic (D2-mdx) mouse gastrocnemius/plantaris muscle