Global gene expression profile of colon biopsy samples from healthy patients and colorectal cancer.
Mutations in Integrator complex subunit 6 (INTS6) correlate with different types of tumors. Lack of Integrator complex function, measured by an increased level of unprocessed snRNA, is a prognostic biomarker and correlates with a poorer prognosis in colorectal patients. The transcriptomic profile of all analyzed colorectal tumors shows a strong alteration of the metabolic state of tumor cells compromising standard energy production by mitochondrial respiration, known as the Warburg effect. In addition, there is a significant upregulation of genes involved in extracellular matrix organization and collagen rearrangement. This is consistent with tissue reorganization in a growing tumor forming a polyp. Crossing the molecular data generated in this study with the follow-up of patients from population screening indicates that population screening combined with early typing of tumors appear to be the most efficient way to increase patient survival. Overall design: To investigate if unprocessing snRNAs serves as a prognostic biomarker in colorectal cancer, a cohort of 90 patients showing different degrees of illness and 10 healthy individuals were selected from the San Pedro Hospital's Digestive System Department (La Rioja, Spain) based on the appearance of a lesion suspected to be of tumor origin during a diagnostic colonoscopy Colorectal tumors were classificated based on INTS6 levels and snRNA processing Transcriptomic analysis was performer from samples from different groups