A novel association of EZH2 and E2F1 promotes breast cancer cell proliferation through chromatin remodeling [RNA-Seq]
This study provides transcription, histone modifications, chromatin accessibility in MDA-MB-231. We identified the non-canonical role of EZH2 by analyzing mutli-omics data. Overall design: RNA-Seq were performed to survey transcriptomics in MDA-MB-231. For knockdown experiment, cells at approximately 70-80% confluency were transduced with the lentivirus for 24 h and selected with puromycin (2 µg/ml) for 72 h. For the drug treatment, cells were exposed to DMSO or GSK126 (Tocris Bioscience) for 72 h.