The MMP-2 histone H3 N-terminal tail protease is selectively targeted to the transcription start sites of active genes [RNA-seq]
Our study examines the role of MMP-2 mediated Histone H3NT proteolysis in U2OS cells. We find binding of MMP-2 at all active protein coding transcription start sites, and examine the role it plays in local chromatin regulation and changes in gene expression. Overall design: RNA-seq of U2OS WT cells and MMP-2 KD cells (2 different shRNAs, 23 and 26) at three different stages of confluency