Differentially expressed genes after overexpression of calcium homeostasis regulating miRNA
he test was partitioned into eleven groups: control (HEK293 cells transfected with pCDNA3.1(+)-mScarlet vector) and HEK293 cells transfected with each of 10 pre-miRNAs. Cells were seeded in 6-well plates. Then, control vectors or pre-miRNAs were transfected into cells. After 48h transfection, RNA extraction and sequencing were performed. Subse-quently, high-throughput sequencing (paired-end 150 nt) was performed on the Illu-mina NovaSeq 6000 platform.