Genetic abnormalities in the pathogenesis of diabetes and it's complication
This project is a focused investigation into the inherited factors contributing to Type 2 Diabetes (T2D) and, critically, its severe microvascular complication, Diabetic Nephropathy (DN). Recognizing T2D as a major global healthcare burden driven by complex genetic and environmental interactions, the study aimed to precisely map the underlying genetic risk loci for DN in a cohort of 26 clinically diagnosed patients (8 females, 18 males) from Tabuk. The core of the methodology involved Whole Exome Sequencing (WES), a high-throughput genomic technique performed on the Illumina NovaSeq 6000 platform. WES allows for the comprehensive interrogation of all protein-coding regions of the genome, enabling the identification of subtle but functionally significant variations that might predispose an individual to DN.