Transcriptomic changes following NR1D1 modulation in thyroid cancer cell lines
Nuclear receptor subfamily 1 group D member 1 (NR1D1), also known as Rev-Erba, is a circadian clock component that functions as a transcriptional repressor which coordinates circadian rhythm and cell metabolism. Altered circadian clock characteristics have been shown in malignant thyroid neoplasms, and NR1D1 is one of the recurrence-associated genes in papillary thyroid cancer. We aimed to investigate the biological role of NR1D1 in thyroid cancer cells. Overall design: Human thyroid cancer cell lines 8505C and CAL-62 were transfected with either a siRNA against NR1D1 or a control siRNA, whereas TPC-1 and FTC-133 cells were transfected with either an NR1D1-overexpressing vector or an empty vector. Cellular RNA was subjected to RNA sequencing.