Investigation of transcript 3' ends in Campylobacter jejuni wildtype and ribonuclease mutants
Termination site sequencing (term-seq) (Dar et al., 2016) was used to map transcript 3' ends and potential processing sites in Campylobacter jejuni NCTC11168 wildtype and ribonuclease deletion strains. Overall design: Total RNA was extracted from C. jejuni NCTC11168 WT and RNase III (rnc, Cj1635c), RNase Y (rny, Cj1209), PNPase (pnp, Cj1253), and RNase R (rnr, Cj0631c) deletion strains grown to exponential phase, genomic DNA was removed with DNase I and rRNA depleted, cDNA libraries were prepared at Vertis Biotechnologie AG, Freising, Germany, deep-sequencing was performed on an Illumina NextSeq 500 instrument