Mus musculus Raw sequence reads
The successful progression of meiosis prophase I requires to integrate information from the structural and molecular levels to coordinate the changes of chromosome structure and biochemical events. In this study, we show that ZFP541 and KCTD19 work in the same genetic pathway to regulate the progression of male meiosis and male fertility. The knockout male mice show various structural and recombination defects including detached chromosome ends, aberrant localization of axis components and recombination proteins, and globally altered histone modifications. Further analyses from RNA-seq, ChIP-seq, and ATAC-seq data not only provide evidence for the above defects but, more importantly, elucidate their role in regulating transcription cascade, and reveal their potential role in directly modulating chromatin reorganization. These results provide a coherent model that ZFP541/KCTD19 simultaneously regulate transcription program and chromatin organization for the coordinate progression of meiosis prophase I at chromosome structural and biochemical levels.