Assay for transposase-accessible chromatin with sequencing (ATAC-seq) after various treatments in T47D cells
This experiment identifies the primary estrogen response genes in T47D cells. Overall design: T47D cells were either hormone starved for 3 days and then treated with estrogen or a vehicle control for 1 hour, or grown in complete media for 1 day and then treated with fulvestrant, raloxifene, tamoxifen, or a vehicle control for 1 hour.