Comparison of miRNA in follicular fluid, miRNA in granulosa cells, and mRNA in granulosa cells
The follicle is a microenvironment filled with follicular fluid (FF), containing various factors essential for oocyte development. MicroRNA is the common non-coding RNA abundantly contained in FF, and certain miRNA concentrations are closely associated with the developmental ability of the enclosed oocyte in same follicles. Granulosa cells (GCs) within the follicle are the source of miRNA production, but the selective production and secretion mechanism remain unclear. In this project, we extracted miRNA from FF and GCs and mRNA from GCs collected from individual cows, and performed small RNA-seq and RNA-seq. This data provides insights into the synthesis of miRNA in GCs and their secretion into FFs.