Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 gene expression in 2D culture format
Four logs of ZJ-101 were tested on 2D cultures of MDA-MB-231 cells to identify relevant mechanistic pathways through differentially expressed gene analysis Overall design: Triplicate dishes of 2D cultured MDA-MB-231 cells were treated for 6 hours with four doses (0.5, 5.0, 50.0, 500.0 nM) of ZJ-101 and processed for RNA-Seq DEG analysis