Gene expression profile at single cell level of kdrl+ FACS sorted cells from WT or flt1 mutant Tg(kdrl:EGFP) zebrafish embryos (3dpf).
scRNA-seq was used to investigate the different endothelial cell population and differential gene expression between arterial and venous cells. Overall design: Kdrl positive cells of WT and flt1 mutant Tg(kdrl:EGFP) embryos were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of GFP signal and processed for single cell RNA-Seq using 10X Genomics Chromium droplet based system