Unravelling tRNA Fragments in DENV Pathogenesis: Insights from RNA Sequencing
tRNA-derived small RNA including tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs) plays significant roles in the various molecular mechanisms that underlie certain human diseases. of the generation of tRFs/tiRNAs and their potential roles during Dengue virus (DENV) infection is not yet known.  Here, we have performed small RNA sequencing to identify the generation and alterations in tRF expression profiles of DENV infected Huh7 cells. Our results suggest that 733 tiRNAs/tRFs were found to be differentially expressed during DENV infection. Interestingly, 3'tRF population were found to be upregulated and i-tRF population were found to be downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed to analyze the impact of differentially expressed tRFs on DENV pathogenesis. Our results suggest the significant involvement in transcriptional regulation via RNA polymerase II promoter and metabolic pathways. Overall, our study contributes significantly to our understanding of the roles played by tRFs in the complex dynamics of DENV infection. Overall design: we conducted a comprehensive RNA sequencing analysis to decipher the distinctive patterns of tRFs/tiRNAs in DENV-infected Huh7 cell lines relative to mock-infected controls. For RNA sequencing, triplicate samples were provided of Mock infected Huh7 cells, 0hr DENV infected Huh7 cells (i.e. 2 hrs post adsorption) and48hrs DENV ijfected Huh7 cells.