Analytic optimization of Plasmodium falciparum marker gene haplotype recovery from amplicon deep sequencing of complex mixtures
Mixtures of up to 10 parasite strains at known proportions and across three parasite density categories, amplified and sequenced in triplicate with Illumina MiSeq (v3 300PE) across polymorphic segments of five distinct markers (ama1, csp, msp7, sera2, and trap).