The effect of endoscopic pretreatment drugs on the microbiota profile in gastric fluid microbiota analysis
Microbial DNA was extracted from the gastric fluid samples using a previously reported protocol [Said HS et al. DNA Res. 2014]. The V1-V2 region of 16S rRNA was amplified through a polymerase chain reaction using the extracted DNA and a universal primer pair (27Fmod, 5'-AGRGTTTGATYMTGGCTCAG-3'; 338R, 5'-TGCTGCCTCCCGTAGGAGT-3') and a unique 8-bp barcode for each sample. The amplicon was purified using AMPure XP beads (Beckman Coulter, CA, USA) and sequenced using a MiSeq Reagent Kit v3 (600 cycles).