irCLIP-seq of DDX24 in HUVECs
DEAD-box (DDX) RNA helicases play crucial roles in gene regulation by interacting with RNAs and influencing RNA fate and function. Previously, we have associated DDX24 with vascular development, but its precise role in RNA metabolism remains unclear. In this study, we demonstrate that DDX24 modulated endothelial cell functions by binding to and regulating mRNAs crucial for vascular morphogenesis and angiogenesis. We further identify DDX24-bound mRNAs in endothelial cells using crosslinking immunoprecipitation (CLIP) sequencing. Mechanistically, DDX24 facilitates the decay of the target mRNAs, such as CLEC14A and ERG, by recruiting the deadenylase complex CCR4-NOT. These results establish a link between DDX24-dependent regulation of mRNA stability and endothelial cell function, providing novel therapeutic targets for angiogenesis. Overall design: To investigate the role of DDX24 in vascular endothelial cells, CLIP-seq of HUVEC with DDX24-FLAG transfected followed by FLAG pull-down. We then identify DDX24-binding targets using data obtained from CLIP-seq of HUVECs.