Ribotag-RNA sequencing data using Csde1-KD dorsal root ganglion(DRG) neurons
We found that the expression of various ribosomal protein genes is regulated at the translation level, and the post-transcriptional regulator CSDE1 controls their association with ribosomes after sciatic nerve injury. This sequencing data shows neurons without CSDE1 exhibit a deficiency in the recruitment of ribosomal protein mRNAs to ribosomes. Overall design: Adult DRG neurons from the RiboTag;Advillin-Cre mice were cultured after giving the pre-conditioning effects induced by sciatic nerve injury 24 hours prior to DRG tissue dissection. Cultured DRG neurons were infected with AAV-control or AAV-shCsde1 four hours after plating. Neurons were collected at DIV3, and their RNAs were extracted from the anti-HA immunoprecipitated lysates.