Effect of methanol fixation on single cell RNA sequencing of the murine dentate gyrus.
We evaluated the effect of methanol (MeOH) fixation on adult murine dentate gyrus (DG) single cell suspensions. Overall design: Freshly and methanol-fixed neuronal and non-neuronal cells from the DG of adult C57BL/6J mice were isolated by Fluorescence-activated cell sorting (FACS), according to the presence or absence of Sytox AADvanced™ Dead Cell Stain, and analyzed using SOrting and Robot-assisted Transcriptome SEQuencing (SORT-seq), a partially robotized version of the CEL-seq2 protocol.