iPS2-10X-seq experiment on specific clones targeting with an shRNA either SMAD2 or B2M
We transfected hiPSCs WTC11 with specific shRNAs targeting SMAD2 or B2M. hiPSCs transfected were selected, cultured, and clones were picked and expanded for follow-up validations. Among these validations, we obtained this scRNAseq dataset. We cultured hiPSCs and differentiated them into cardiac organoids for 7.5 days with and without Tetracycline treatment. This experiment allowed to identify the role of SMAD2 during cardiac differentiation.