Transcriptome data of bone marrow hematopoietic stem cells (HSCs) with IGF-1 treatment
Insulin-like growth factor-1 (IGF-1) is known as a hematopoietic factor which impacts hematopoietic reconstitution and facilitates thrombopoiesis, although its direct effect on hematopoietic stem cells (HSCs) remains unclear. Here, we show that IGF-1 rapidly prompts megakaryocyte (Mk)-lineage differentiation of HSCs by enhancing mitochondrial activity in HSCs. To identify the phenotype switching and function variation in HSCs with IGF-1 treatment, we performed RNA-seq of HSCs from the bone marrow of mice with single-dose IGF-1 treatment (IGF-1) and their age-matched control mice (Ctrl). Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of BM HSCs (CD34-Flk2-Lineage-Sca1+c-Kit+) of control or IGF-1 mice.