Population Genomics and Environmental DNA Analysis of the Critically Endangered Acipenser dabryanus for Conservation Prioritization
This project integrates amplicon sequencing of 53 sturgeon individuals(Including 51 Acipenser dabryanus, 1 Acipenser sinensis and 1 Acipenser ruthenus) and environmental DNA (eDNA) analysis from 46 sampling sites in the Yangtze River. The study aims to: Characterize population genetic diversity and identify critical adaptive loci using multiplexed MNP (Multiple Nucleotide Polymorphism) markers. Assess the efficacy of eDNA-based monitoring for detecting Acipenser dabryanus in fragmented habitats. Provide genomic resources to inform conservation strategies for this critically endangered species. Methods: Individual Genomes: Genomic DNA was extracted from muscle tissues, amplified via species-specific MNP primers, and sequenced. eDNA Workflow: Water samples were filtered, DNA was extracted using a phenol-chloroform protocol, and target loci enriched by PCR prior to sequencing. Data include: Raw sequencing reads (FASTQ) for individual genomes. This study addresses the urgent need for genomic tools in freshwater fish conservation and pioneers eDNA applications in sturgeon monitoring.