Effect of Phb2 depletion in retinal photoreceptors on gene expression in the bulk retina of C57BL/6J mice
To explore the functions for Phb2 gene in early retinal degeneration, we performed RNA-seq analysis on the bulk retinas from control and photoreceptor-specific knockout Phb2 mice at 6 weeks of age. Compared with control mice, there were 778 differentially expressed genes (log2 |fold change|> 1, log10 adjusted p-values < 0.05) identified to be associated with distinct biological processes. 585 genes were significantly upregulated and 192 genes were significantly downregulated in photoreceptor cell-specific knockout Phb2 mice. The KEGG pathways analysis of up-regulated and down-regulated genes and ranking the enrichment pathways according to the corrected p-value.To explore the critical signaling pathways that might be affected by knockout Phb2 in retinal photoreceptors, we performed RNA-seq analysis on 6-week-old C57BL/6J photoreceptor cell-specific knockout Phb2 mice and control mice. Compared with control mice, there were 778 differentially expressed genes (log2 |fold change|> 1, log10 adjusted p-values < 0.05) identified to be associated with distinct biological processes. 585 genes were significantly upregulated and 192 genes were significantly downregulated in photoreceptor cell-specific knockout Phb2 mice. The KEGG pathways analysis of up-regulated and down-regulated genes and ranking the enrichment pathways according to the corrected p-value. Overall design: retina were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.RNA libraries were prepared for sequencing using standard Illumina protocols