Translating ribosome affinity purification (TRAP) and RNA-seq identified cilia dependent dysregulated genes in early cystic Pkd1 models
To identifying cilia membrane proteins would reveal the designated renal physiological processes controlled by cilia, and validation of its function in the pathophysiology of ADPKD will yield therapeutical targets. In this study, we used RiboTag mice , a Cre recombinase dependent ribosome protein L22-HA gene knockin mice that enable ribosome associated transcriptome isolation, to cross to Pkd1 and Ift88;Pkd1 model to identify cilia dependent differential expression genes (DEGs) in tubular cells at early cystic stages.