Transcriptome analysis of the venom gland of Centruroides hirsutipalpus
Venom glands from the telsons of four specimens of the Mexican scorpion Centruroides hirsutipalpus consisting in 2 males and 2 females were dissected and the total RNA was extracted. A cDNA library was constructed and subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 2,553,529 reads were obtained by the RNA-seq procedure. The Trinity assembly resulted in a total of 44,579 transcripts. After bioinformatic analysis, 141 transcripts were identified potentially coding for scorpion venom components.