Comparative Transcriptome Analysis of Peripheral Blood in High-Risk Preeclampsia versus Normotensive Pregnancies
This study identifies peripheral blood transcriptomic signatures associated with high-risk preeclampsia (PE). RNA sequencing of 10 PE patients (clinically diagnosed with hypertension and proteinuria) and 10 matched normotensive controls revealed differentially expressed genes (DEGs) enriched in angiogenesis, immune regulation, and endothelial dysfunction pathways, suggesting their potential as early biomarkers for PE. Overall design: Peripheral blood samples from PE high-risk and control groups were collected in EDTA anticoagulant tubes, immediately mixed with TRIzol Reagent by vigorous vortexing to ensure complete cell lysis and RNA stabilization. Transcriptome profiling was performed using the Illumina NovaSeq 6000 platform with paired-end 150 bp sequencing, generating ~40 million reads per sample to achieve high coverage depth. Differential gene expression analysis focused on PE-associated pathways (angiogenesis, immune dysregulation) using DESeq2 with thresholds of |log2FC| =1 and adjusted p <0.05.