Endophytic Fungi of Fuzi
We have previously conducted a research on Fuzi from three different regions, Chenggu, Liangshan, and Lijiang, to exploring the relationship between Alkaloid Accumulation and biotic and abiotic environmental factors. The results showed that the content of benzoylaconine, benzoylmesaconine, and monoester-type alkaloids were positively correlated with the abundance of Acinetobacter, soil pH, and Al3+ content, while negatively correlated with the abundance of Aspergillus. The content of hypaconitine was positively correlated with the abundance of Bradyrhizobium, while the content of benzoylhypaconine and hypaconine were negatively correlated with the abundance of Athelia and Fusarium (Qiu et al. 2021). However, the limited sampling points and collected samples cannot fully reflect the relationship between the growth and secondary metabolites accumulation of Fuzi and its habitat and endophytic fungi.Based on previous research, we conducted a more extensive sampling to explore the uncertainty surrounding the specific endophytic fungi and soil nutrients that influence the synthesis of aconite alkaloids in Fuzi. By collecting Fuzi samples and rhizosphere soil from various regions in China, we conducted an analysis to examine the effects of Fuzi endophytic fungi and soil nutrients on the alkaloid contents. Our objective is to provide valuable insights for the research and development of field management techniques that can enhance the content of Fuzi. Ultimately, this research aims to establish a better understanding of the relationship between alkaloid contents, endophytic factors, and external environmental factors, with the content of key secondary metabolites playing a crucial role in evaluating the quality of medicinal plants.