Transcriptional activation of C19MC in 293T and DICER1-knockout 293T cells
To investigate the role of the C19MC SINEs and distinguish them from the C19MC miRNAs, we utilized CRISPR/dCas9 SAM system with 759-sgRNA to transcriptionally activation the entier cluster in 293T and in DICER1 knockout 293T cells compared to control backbone sgRNA transfected cells.