Analysis of A375 and SKMEL-103 cells after treatment with with DMSO as control or BAY-850
To identify the gene expression changes in NRAS mutant cell line SKMEL-103, and BRAF mutant cell line A375 upon BAY-850 treatment, we analyzed these cell line with either control DMSO or BAY-850 treatment via RNA sequencing. Overall design: SKMEL-103 and A375 cells were plated, samples were either treated with DMSO or BAY-850 (5-micromolar for 24 hours), cells were harvested and were used to prepare the total RNA, followed by RiboRNA depletion with random priming and RNASeq Illumina Hi-Seq 2500 platform, with three biological replicates for each sample.