curdlan intakes improves gut microbial composition
Male C57BL/6J mice were divided into two groups of similar average body weight [cellulose-fed group and curdlan-fed group] for 12 weeks in a factorial design. Feces were collected at after treatments. Fecall DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq.