The First Single-Cell Sequencing of Plasmodiophora brassicae Reveals Genetic Diversity and Clonal Dynamics
A method was developed to enzymatically remove cell walls from dormant spores of Plasmodiophora brassicae, enabling the first single-cell sequencing of this clubroot pathogen, revealing high genetic diversity among ~4000 protoplasts. The predominance of distinct genotypes suggests clonal reproduction, complicating single-gene resistance management in brassica crops.