Methionine metabolism regulates T cell fate by KCa3.1 activity (ATAC-Seq)
We found that methionine availibility during T cell activation regulates rapid NFAT1 activation, thereby governing T cell fate. Further, acute methionine limitation didn't affect global histone methylation status stating the crucial role of methioinine during early TCR activation. Overall design: ATAC-Seq of 12 samples : 24 hrs activated OT-I CD8+ T cells in either 0.1mM methionine (control) or 0.03mM Methionine for 30 min, before methionine restoration in 0.03mM to control concentration (n=3/group) Other samples are KCa3.1 WT or mutant, activated for 24 hrs with SIINFEKL before processing (n=3/group).