Transposon sequencing on M. tuberculosis with Pretomanid and BDM88927
Transposon sequencing on M. tuberculosis with Pretomanid and BDM88927 to identify the mechanism of action. Sample A was DMSO treated. Sample B was treated with 50 ng/mL of pretomanid. Sample C was treated with BDM88927 at 10 microM. Sample E was treated with 50 ng/mL of pretomanid and with BDM88927 at 10 microM. Sample D was treated with pretomanid at 1 microg/mL. Sample F is the Tn-library preculture used to inoculate all samples. All samples (except for F) were prepared as five replicates. Each replicate was amplified and sequenced five times at library preparation step.