The response of A549 transcriptome to RNA Pol II slowdown with alpha-amanitin and DRB
According to current estimates, more than 40% of nascent introns are spliced co-transcriptionally and, consequently, the speed of RNA polymerase II elongation must fundamentally affect the rate of RNA folding, spliceosome assembly, and the resulting mature transcript. RNA-Seq data was generated using RNAPII elongation inhibitors DRB and a-amanitin in A549 cells and used to look at genome-wide splicing changes Overall design: 5x RNA-seq (DMSO, 10 ug/ml DRB, 25 ug/ml DRB, 1 ug/ml a-amanitin, 2 ug/ml a-amanitin) in A549 cells