Single-cell RNA sequencing of mouse hearts under normal fat diet and high fat diet conditions
To investigate transcriptional changes in the heart induced by diet, we performed single-cell RNA sequencing on pooled mouse hearts from normal fat diet (NFD, 10% kcal fat) and high fat diet (HFD, 60% kcal fat) groups using the Parse Biosciences Evercode WT Mini v3 kit. Overall design: Whole hearts from C57BL/6J mice fed either NFD or HFD for 4 weeks (n=3 pooled per group) were processed using the Parse Biosciences Evercode Whole Transcriptome Mini v3 kit for single-cell RNA-seq. Libraries were sequenced on Illumina NovaSeq 6000. Two sublibraries were generated.