Structure of the intestinal flora of the colonic contents of rats with vascular calcification
This study utilized 16S rRNA gene sequencing to comprehensively characterize the microbial community structure within colonic content samples obtained from rats. By targeting the hypervariable regions (e.g., V3-V4) of bacterial 16S rRNA genes, we identified taxonomic profiles at multiple levels (phylum, genus, and species), assessed alpha/beta diversity, and explored potential functional associations between gut microbiota and vascular calcification.