Transcriptomic Landscape of Fibroblasts Under IL-1ß Stimulation [ATAC-seq]
In this study, we investigated the chromatin accessibility changes in human dermal fibroblasts in response to IL-1? stimulation, a key pro-inflammatory cytokine. Using ATAC-seq, we compared the open chromatin landscapes between control fibroblasts and those treated with IL-1?. The data provide insights into the regulatory elements activated during fibroblast differentiation and their potential role in sustaining inflammation within the skin. These findings contribute to our understanding of fibroblast-mediated inflammation in autoimmune diseases like SLE and offer a resource for identifying novel therapeutic targets. Overall design: Human dermal fibroblasts were cultured and treated with IL-1B (10 ng/mL) for 24 hours to mimic the inflammatory conditions. Control fibroblasts were cultured under identical conditions but without IL-1B stimulation. ATAC-seq was performed to map genome-wide chromatin accessibility in both control and IL-1B-treated fibroblasts. The experimental design allows for the identification of differentially accessible regions in the genome, which may reveal key regulatory elements involved in fibroblast activation and their interaction with immune cells. Data were generated in biological triplicates for each condition to ensure robustness and reproducibility.