Transcriptional profiling of small intestine organoids generated from C57BL/6 (WT), Stat1KO, Stat2KO and Stat1KOxStat2KO mice after stimulation with type I, type II or type III interferon.
Small intestine organoids generated from young adult mice were passaged once on day 3. Medium was changed again on day 7 and, one day later, organoids were stimulated for 20 hours with either 25ng/ml IFN-ß (R&D Systems), or 25ng/ml IFN-? (BioLegend) or with 200ng/ml IFN-?2 (PeproTech) or were left untreated (unstimulated group).