Assessment of myogenic maturation for human iPSC-derived iPAX7 myogenic progenitors in vitro and in vivo
Here, we transplanted human iPAX7 myogenic progenitors into skeletal muscles of non-dystrophic and dystrophic mice and compared the transcriptional landscape of human donor-derived myofibers with respective in vitro-differentiated iPAX7 myotubes. Pairing bulk RNA sequencing with computational deconvolution of human reads, we were able to pinpoint key myogenic changes that occur during the in vitro-to-in vivo transition, confirm developmental maturity, and consequently further suggest the utility of cell-based therapies. Overall design: We performed gene expression profiling for data obtained through bulk RNA-sequencing of 14 uniques samples. Samples included non-dystrophic or dystrophic skeletal muscle injected with or without iPAX7 myogenic progenitors, as well as terminally differentiated myotubes in vitro.