transcriptome analysis in canine histiocytic sarcoma cell lines
RNA sequence analysis was performed  to investigate the relationship between molecular pathology and sensitivity to molecular target drugs on canine histiocytic sarcoma cell lines. RNA was extracted from a canine histiocytic sarcoma cell line, and the RNA integrity number was calculated using an Agilent 2100 Bioanalyzer. After extracting mRNA using the poly(A) method and preparing the library, 26.7 M reads per sample were read using NovaSeq 6000 with 150 bp paired-end.