Comparison of methods for the optimal recovery of the human fecal virome
We evaluated different protocols for shotgun metagenomic sequencing of the human fecal virome. Human fecal samples were processed using multiple strategies involving viral enrichment, nucleic acid extraction, amplification, and library preparation methods. The resulting libraries were sequenced and compared to assess protocol-specific impacts on taxonomic and functional assignment, contig quality, viral diversity, and genome structure.