single-cell RNA-sequencing of murine intestine 24 hours following acute p53 activation due to Mdm2-loss (Mdm2FM/- Cag-CreERTg (FMTg)) compared to intestine that retain Mdm2 (Mdm2+/- Cag-CreERTg (Tg))
FMTg (Mdm2FM/- Cag-CreERTg) and Tg (Mdm2+/- Cag-CreERTg) mice were administered 6mg/40g of Tamoxifen dissolved in corn-oil and sacrificed 24 hours after injection. Single cells were isolated from fresh small intestine samples to compare single cell transcriptomes between intestine with high or control p53 activity Overall design: One FMTg small intestine and one Tg small intestine were isolated from mice 24 hours following a single 6mg/40g tamoxifen injection. Single cells were isolated from fresh tissues and then processed through the 10X genomics platform