Gastric epithelial cells infected with Helicobacter pylori for 24 hours
Helicobacter pylori (H. pylori) infects nearly half of the global population. H. pylori infection is associated with various gastric diseases and is a recognized causative factor for gastric cancer. The mechanisms underlying the persistent colonization of H. pylori and its role in the development and progression of gastritis-associated carcinogenesis remain unclear. To investigate the pathogenic mechanisms of H. pylori infection, we performed RNA sequencing (RNA-seq) on H. pylori-infected gastric epithelial cells and analyzed the gene expression patterns induced by H. pylori infection, aiming to provide critical insights for understanding its disease-driving pathways. Overall design: Compare the gene expression profiles between H. pylori(ATCC26695) infected and uninfected gastric epithelial cells under the condition of a multiplicity of infection (MOI) = 100 for 24 hours.