Response of Klebsormidium treated with glutamine and GS-inhibitors
To determine transcriptional response to GS-inhibiting herbicides, RNAseq analysis was perform on Klebsormidium treated with 10mM glutamine, 100uM phosphinothricin, or 5uM methionine sulfoxime for 48h. Treatment was performed by incubating cultures with the compounds in Full Yamagami media under low light at 22C with shaking at 98rpm.