Metabarcoding diet analysis of marine cladocerans, Penilia avirostris and Psuedevadne tergestina
Metabarcoding diet analysis targeting 18S and 16S rRNA were performed to reveal feeding habits of marine cladocerans Penilia avirostris and Pseudevadne tergestina. Zooplankton samples were collected in central part of Suruga Bay, Japan. Twenty individuals each of Pe. avirostris and Ps. tergestina were randomly sorted, and dissected to obtain their gut. DNA of prey organisms contained in gut contents were extracted and amplified. Sequencings were performed using NGS MiSeq.