CITE-seq of murine bone and marrow cells
To investigate heterogeneity of CD24 osteolineage cells at the single-cell level, we performed CITE-seq analysis with the CD24 antibody on bone and marrow mesenchymal cells from mouse femurs and tibias Overall design: We isolated bone and marrow cells from the femurs and tibias of 2 mice (n=1 24-month-old, n=1 6-month-old), stained with CD24 TotalSeq antibody, depleted of immune cells (Lin-) by MACS and enriched for live mesenchymal cells (CD45-SyTOX-) by FACS, and performed 10X Genomics CITE- and single-cell RNA-sequencing