Transcriptome profiling of radiotherapy-induced senescent tumor cells and BRD4 degrader intervention
This BioProject contains raw bulk RNA sequencing data generated from Panc02 murine pancreatic tumor cells to characterize transcriptional changes induced by radiotherapy and their modulation by BRD4 degradation. Cells were exposed to radiotherapy at 8 Gy and then treated with either vehicle or a BRD4-targeting peptide PROTAC degrader (BRD4d, 1.0 uM) for 2 days, alongside non-irradiated controls. Three biologically independent replicates were collected per condition. The dataset supports analyses of senescence-associated, immune checkpoint-related, apoptosis, and proliferation gene programs under radiotherapy and BRD4 degrader intervention. Raw FASTQ files and sample-level metadata are provided.