Cataract ncRNA
A cataract is a clouding of the lens. This distorts or blocks the passage of light through the lens, causing cloudy or blurred vision, other visual problems, and even blindness. Three transparent and three cataract patients lens were collected and total RNA were obtained from eye bank of the First Affiliated Hospital Xi'an Jiaotong University. Each sample was separated equally for lncRNA and mRNA library construction and circRNA library establishment. LncRNA and mRNA sequencing were completed first and ribosome free chain specific Transcriptome library was then constructed for circRNA sequencing. RNA sequencing was conducted using Illumina novaseq 6000.