The P-body protein 4E-T regulates the balance between cell genesis and establishment of the postnatal neural stem cell pool by repressing mRNA translation [Cpeb4_VSVZ]
To investigate the targets of Cpeb4 in the postnatal V-SVZ We performed RNA immunoprecipitations (RIPseq) on V-SVZ lysates anti-Cpeb4 or control anti-IgG antibodies, isolated and sequenced the resulting RNA Overall design: The SVZ was collected and lysed from P7/P8 CD1 animals, and immunoprecipitations were performed using anti-Cpeb4 or anti-IgG control antibodies, and the associated mRNA was isolated