Effect of hypoxia on gene expression in Hep3B cells
Hypoxic microenvironment plays a critical role in solid tumor progression. More and more protein coding and non-coding RNA genes have been identified as hypoxia-regulated genes in HCC. Thus, we attempted to identify more novel hypoxia-regulated genes in HCC with Hep3B cells exposed to 20% or 1% oxygen. Overall design: Hep3B cells were exposed to 20% or 1% oxygen for 24 hours, then the total RNA was isolated for subsequent test.