RNA-Seq Analysis of Equine Intestinal Epithelial Models: 2D Intestinal Organoids and 3D Organoid-Derived Air-Liquid Interface (ALI) Cultures
This project aims to perform transcriptome sequencing (RNA-Seq) of two types of equine intestinal epithelial models to explore their gene expression profiles and molecular characteristics. The sequencing samples include 2D equine intestinal organoids (2D EIO) and air-liquid interface (ALI) models established based on 3D equine intestinal organoids, with 3 biological replicates for each model (total 6 samples). Transcriptome data will be generated to identify differentially expressed genes, enriched pathways, and molecular mechanisms associated with the structural and functional differences between 2D EIO and 3D organoid-derived ALI models. These data will provide a valuable molecular basis for further studying equine intestinal physiology, intestinal epithelial cell function, and the application of intestinal organoid models in equine-related biological and medical research.