16S rRNA gene amplicon sequencing of rat gut microbiota in adenine-induced chronic kidney disease with emodin intervention
This project aims to investigate the modulatory effect of emodin on gut microbiota in a rat model of chronic kidney disease (CKD). Male Sprague-Dawley rats were fed a 0.75% adenine diet for 4 weeks to induce CKD, with or without concurrent oral administration of emodin (40 mg/kg/day). Fecal samples were collected from three groups: Control (standard diet), CKD (adenine diet), and Emodin (adenine diet + emodin), with 6 biological replicates per group. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 338F and 806R, and paired-end sequencing (2 x 250 bp) was performed on the Illumina NovaSeq 6000 platform. The raw sequence data will be used for microbial diversity analysis, taxonomic composition profiling, and functional prediction to elucidate the gut-kidney axis mechanism of emodin in CKD.