H3K4me3 ChIP-seq of C57BL/6 mouse embryonic stem cells with folate deficiency
In order to investigate whether folate deficiency affects histone H3K4me3 enrichment and then affecting the activity of Wnt signaling pathway, we performed ChIP-seq of H3K4me3 on folate-free embryonic stem cells and normal embryonic stem cells, respectively. Our sequencing data provides a new theoretical basis for the effect of folate on histone modification. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone variants H3K4me3 (Cell Signaling Technology; catalog number: 9751s; lot number: 14.)