Bulk RNA-seq of wild-type and Nlrp3-/- bone marrow-derived macrophages with or without LPS and ATP costimulation
In order to investigate the effect of NLRP3 on the transcription profile of inactive macrophages and active macrophages with LPA and ATP combined stimulation. Overall design: RNA-seq profiling of wild-type and Nlrp3-/- bone marrow-derived macrophages without stimulation or with 4 hours of LPS preconditioning and subsequently 30 min of ATP stimulation.