TA-cloning based sequencing of circular RNAs in human glioma cell
Circular RNAs (circRNAs) are a covalently closed class of non-coding RNAs that play important roles in cancer pathogenesis, including glioma. This study aimed to experimentally identify and validate circRNAs expressed in human glioma cells.  Total RNA was isolated from human glioma cell lines. To enrich for circular transcripts, the RNA was treated with RNase R to degrade linear RNAs. cDNA was then synthesized, and divergent primers were used to amplify potential back-splice junctions by PCR. The resulting PCR products were cloned by Sangon biotech, and positive clones were confirmed by Sanger sequencing.  This submission contains the nucleotide sequence of a circRNA molecule, identified as hsa_circ_0001361 ( derived from the FNDC3B gene ), in human glioma cells.The sequence provides direct experimental evidence for the existence of this circular transcript via the detection of a characteristic head-to-tail back-splice junction. This data contributes to the understanding of the circRNA landscape in glioma and may facilitate future research into its biological function and clinical significance.