RNA Sequencing of Arabidopsis seedlings upon GR244DO treatment
The sterilized and stratified wild typle, s678 and tcpSEP seeds were grown on 1/2 Murashige and Skoog media plates under LD conditions (8 h light/16 h dark cycles) for 10 days. Whole seedlings were carefully transferred to half-strength MS liquid medium without injury and cultured for 2 h, then treated with GR244DO or dimethyl sulfoxide (solvent) in the greenhouse for 4 h. Three duplicate samples were taken for each genotype. Approximately 100-mg whole seedlings were harvested for total RNA extraction and RNA-seq library preparation. The libraries were sequenced on an Illumina NovaSeq 6000 platform by generating 150 bp paired-end reads. The fastp (version 0.23.2) was used for the quality control of raw reads. Then the clean reads were mapped to the Arabidopsis genome (TAIR10) by Hisat2 (version 2.1.0). Expression counts were generated by featureCounts of Subread (version 2.0.1) with default parameters. The R package DEseq2 (version 1.44.0) was used for gene differential expression analysis.