Gene expression profile of microglia following traumatic brain injury and nasal anti-CD3 treatment at acute and chronic timepoints
Bulk RNA-sequencing was performed to characterize the gene expression profile of microglia at acute and chronic timepoints following traumatic brain injury and nasal anti-CD3 treatment. We further investigated how the chronic microglial transcriptomic profile is modulated following traumatic brain injury and nasal anti-CD3 treatment in female mice with severe TBI, and in male mice with a delayed administration of treatment post-injury. Overall design: Bulk RNA-sequencing analysis was performed on sorted microglia single cell suspensions from the ipsilateral hemisphere of the mouse brains using the microglia-specific 4D4+ antibody at 7 days and 30 days following traumatic brain injury. Preparation for RNA libraries for sequencing was performed using standard Illumina protocols. For the delayed treatment experiment, the therapeutic window of nasal anti-CD3 treatment was delayed to 3 days following injury.