Restriction-site associated DNA sequencing of Nagoya and White Plymouth Rock meat-type chickens and their F1 and F2 populations
By crosses between 3 males of Nagoya and 5 females of White Plymouth Rock, an F1 population of 13 chickens were obtained. An F2 population of 239 chickens were produced from crosses between the F1 chickens obtained. Restriction-site associated DNA sequencing of genomic DNAs extracted from blood cells of all individuals was performed using two restriction enzymes of EcoRI and BglII to develop a high-density SNP linkage map in chickens.