Antigen exposure reshapes chromatin architecture in central memory CD8+ T cells and imprints enhanced recall capacity [RNA-seq]
Compared with naïve CD8+ T cells, antigen-experienced effector and central memory CD8 T cells show extensive CTCF redistribution and 3D genome reorgainzation, which underlie the transcriptomic diversification as well as recall capacity in response to secondary challenges. Overall design: CreER+ WT or CTCF-floxed P14 CD8+ T cells were first transferred into B6.SJL mice followed by LCMV-Arm infection. After =30 days, the immune mice were treated with tamoxifen for two days, and CD62L+ P14+ central memory (Tcm) cells were sorted, and a portion was stimulated with GP33 peptide for 24 hrs. The unstimulated (resting) and stimulated Tcm cells were subjected to RNAseq analysis