To investigate the effect of TMAO intervention on fatty liver cells in vitro and potential genetic changes
To construct the optimal fatty liver cell model in vitro and use TMAO intervention to observe its effect on fatty liver cells and identify potential key genes for change by transcriptomics. Overall design: Comparative gene expression profiling of RNA-seq data between HepG2 fatty liver cells and HepG2 fatty liver cells with TMAO intervention, three samples in each group.