RNA-seq analysis for Chlamydomonas in lcr1 mutant
To identify genes affected by the LCR1 mutation, RNA-seq analysis was performed in C9 (WT), LCR1 mutant, and the complemented strain. RNA was extracted from cells cultured under high-CO2 (HC) or Very low-CO2 (VLC) for 0.3 h or 2 h. There are two biological replicates of each condition. Sequencing was performed on Novaseq system and consisted of forward and reverse reads.