Idnetification of a new HLA-A*03:01 in a french  voluntary bone marrow donor.
Here we describe a novel HLA-A*03 :01 allele, identified in a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*02:01, *03:01New B*07:02, *73:01 C*07:02, 15:05 DRB1*01:01, *04:05 DRB4*01:03 DQA1*01:01, *03:03 DQB1*02:02, *05:01 DPA1*01:03,*- DPB1*04:02, *104:01 HLA-A*03 :01 New allele differs from A*03 :01 :01 :01 by one nucleotide substitution at position  12 of exon 6 (position  1024 of cDNA), where  base G is replaced by A , inducing Arg instead Gly (GGG>AGG)