Genome wide map of Runx3, Brn3a, Islet1 binding, chromatin accessibility (ATAC) and Histone H3 acetylated on lysine 27 (H3K27Ac) during early development of DRG TrkC neurons
We isolated E11.5 TrkC neurons and profiled chromatin accessibility (ATAC), Runx3, Brn3a, Islet1 binding and Histone H3 acetylated on lysine 27 (H3K27Ac) during early development of DRG TrkC neurons by Cut&Run Overall design: Isolation of GFP expressing TrkC neurons from E11.5 BacE-GFP embryos and performing Cut&Run with anti-Runx3, anti-Brn3a, anti-Islet1 and anti-H3K27Ac antibodies. Performing ATAC assay