The trajectories of pro-neural phenotype switch induced by NGN2, ASCL1, SOX2, and MSI1 in human fibroblasts
Direct pro-neural reprogramming is a conversion of differentiated somatic cells toneural cells without an intermediate pluripotency stage. It is usually achieved via an ectopicexpression (EE) of certain transcriptional factors (TFs) or other reprogramming factors (RFs).Determining transcriptional changes (TCs) caused by particular RFs in a given cell line enables aninformed approach to reprogramming initiation. Here, we characterized TCs in the humanfibroblast cell line LF1 at 5th day after EE of single well-known pro-neuronal RFs NGN2, ASCL1,SOX2, and MSI1.