Transcriptome and TCR analysis of KLRG1+mouse effector tissue Tregs and control Tregs in steady state and neoplastic proximal intestine
Mouse tumorigenesis induces the local accumulation of tissue effector Tregs that resemble the Tregs accumulating in human tumours. We used a model of tamoxifen-induced stabilization of bcatenin in gut epithelial cells, which mimics early events in intestinal tumorigenesis. We analyzed the Treg response by sc-RNA Seq and V(D)J-repertoire profiling and clonality analysis by bulk RNA sequencing of the TCRa chain. For that, we sorted effector tissue and control Treg based on CD25 and KLRG1 expression on CD4+ T cells. We then compared the transcriptome and clonality of these Treg populations in steady state and neoplastic proximal small intestine.