RNA-seq of ventricle and fat tissues of male and female cardiac myocyte-specific estrogen receptor KO mice
Cardiac myocyte-specific ERalpha KO mice were generated to assess the role of ERa in the heart. Female ERaHKO mice displayed a modest cardiac phenotype, but unexpectedly, an the most striking obesity phenotype developed was obesity in female ERaHKO but not male ERaHKO mice. In female ERaHKO mice we identified cardiac dysfunction, mild glucose and insulin intolerance, and reduced ERa gene expression in skeletal muscle and white adipose tissue (WAT). RNA-seq analysis was conducted on the ventricles and WAT of male and female ERaHKO mice to further elucidate the transcriptomic alterations associated with the shift in metabolic profiles in the tissues of interest.