To determine the impact of perinatal MCMV infection on NK cells
To determine the impact of infection on NK cells, splenocytes were isolated from spleens of naive or MCMV-infected mice at day 21 p.i. Following isolation, splenocytes were labeled with anti-CD45, anti-CD3, anti-CD19, anti-NK1.1, and anti-DX5 antibodies, and NK cells defined as CD45+, CD3-, CD19-, NK1.1+, and DX5+ cells, were separated from the mixture using fluorescence-activated cell sorting on FACSAriaIIu.