IRSIP 51 BMS 28 - Cu experiment
A pot experiment was conducted with copper spiked soil to evaluate the synergistic effect of bacterial inoculation and nanoparticles on the phytoremediation of Cu-contaminated soil. Sterilized soil was spiked 374 mg kg-1 of copper. Ryegrass seeds (Lolium perenne) were used as a hyperaccumulator plant. The soil was inoculated with two selected bacterial strains NCCP-2261 (Bacillus safensis subsp. safensis) and NCCP-2265 (Bacillus cereus). The plants were harvested 60 days after seedling, and various physiological and biochemical parameters were examined. Analysis was conducted on soil and plant samples to determine the metal concentration. Bacterial colonization in soil was studied with Illumina Miseq.