Metabarcoding of Calanus symbionts
Zooplankton were collected live each month (except July) throughout 2022. Starved single individuals and pooled samples of Calanus finmarchicus/helgolandicus species samples were metabarcoded with Illumina MiSeq. In addition, water samples were collected and metabarcoded. PCR was done in a single step using fusion primers that are biased against amplifying metazoan sequences.