Transcriptomic profiling of circadian genes in WT mouse tissues (mRNA-Seq)
A goal of a collaboration between the CHDI Foundation and Prof. Jenny Morton at Cambridge University was to identify genes in mice with diurnal/circadian expression patterns. Tissues from wild type mice were harvested every 3 hrs over a 36 hour time period, for a total of 13 time points. Striatal, cortical, hypothalamic and liver samples were harvested, flash frozen, and transferred to Expression Analysis for RNA extraction and mRNA-seq sequencing. Samples passing QC were analyzed to produce high quality, 50-base paired-end RNA-seq results. Overall design: Striatal, cortical, hypothalamic and liver samples were collected from 26-week-old wild type C57BL/6J male mice every 3 hours for 36 hours, number of replicates 2 to 12 for each ZT group. mRNA-Seq was performed.