Heart transcriptional changes with prednisone with inducible cardiomyocyte-specific GR ablation
this dataset encompasses the cardiac transcriptomic changes elicited in myocardial tissues of 4months-old male mice at 4 hours after a single ZT0 prednisone (1mg/kg i.p.) injection, or vehicle, in cardiomyocyte-GR-WT versus cardiomyocyte-GR-KO conditions (inducible, tamoxifen-driven ablation right prior to drug pulse). Overall design: Mice were obtained and interbred from the Jackson Laboratories (Bar Harbor, ME; JAX strain number listed) or from collaborators: Myh6-MerCreMer mice #005657, GR-flox mice #021021. Gene ablation was induced with tamoxifen at ~8 weeks of age using a combination of i.p. (20 mg/kg/day for 5 days; Sigma #T5648) and chow-mediated (40mg/kg for 3 weeks; Harlan #TD.130,860) administration. Analyses were performed after 4 weeks of tamoxifen washout, i.e. at ~16 weeks of age. Drug treatment consisted of single intraperitoneal injection of prednisone (1 mg/kg; #P6254, Sigma-Aldrich, St. Louis, MO). The injectable solution was diluted from a stock (5 mg/ml) in dimethyl sulfoxide (DMSO; #D2650, Sigma-Aldrich, St. Louis, MO) in a 50-µl volume. Injections were conducted at the beginning of the light phase (ZT0; lights on) in mice kept on 12h/12h light/dark cycles. Tissues were harvested at 4 hours post-injection.