RNA-seq of human prostate cancer cell line PC-3M trpv6 knockout versus wild type and PC3M-Luc-C6 wild type versus trpv6 overexpression.
TRPV6 is a membrane protein, a member of the transient receptor potential (TRP) ion channel family. The TRPV6 cation channel is highly selective for Ca2+, with PCa/PNa values greater than 100, playing a crucial role in calcium homeostasis in various cell types. Previous transcriptome analysis indicated that TRPV6 mRNAs were expressed in PCa and that channel expression correlates strongly with the pathology stage and metastasis status. The aim of the present study was to examine calcium-regulated signaling pathways in castration-resistant prostate cancers. Overall design: Three biological replicates were analyzed for each of 2 conditions of PC3M cell line or two biological replicates were analyzed for each of 2 conditions of PC3M-Luc-C6 cell line with different trpv6 expression.