Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: Cut&Tag
This study uses Cleavage Under Targets and Tagmentation (Cut&Tag) chromatin profiling to map genome-wide binding sites of TES (TEAD1 Epigenetic Silencer, an engineered epigenetic silencer factor consisting of KRAB-hTEAD1(1-166)-DNMT3A/3L with V5 tag) and overexpressed wild-type TEAD1 (HA-tagged) in SNB19 glioblastoma cells. Cut&Tag was performed using the CUTANA protocol (EpiCypher). Three biological replicates were prepared for TES (detected via anti-V5) and TEAD1 (detected via anti-HA), with mouse IgG and rabbit IgG as negative controls. Peak calling identified binding sites for both factors, showing that TES largely preserves the DNA-binding specificity of wild-type TEAD1 while converting it into a transcriptional repressor. Overall design: Cut&Tag chromatin profiling of SNB19 glioblastoma cells following the CUTANA CUT&Tag Protocol (EpiCypher): 3 TES replicates (anti-V5, detecting V5-tagged TES construct), 3 TEAD1 replicates (anti-HA, detecting HA-tagged overexpressed TEAD1), 1 mouse IgG control, 1 rabbit IgG control (8 samples total).