Gene expression in 10 d Wild-type and Amh-cre Hdac3 KO Sertoli cells
This study aims to explore the transcriptional changes in Sertoli cells depleted with HDAC3.  Sertoli cells were isolated from wild-type and Amh-cre Hdac3 KO mice at postnatal day 10. Overall design: For each group, we have  biological triplicates. Total RNA was extracted using TRIzol reagent. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.