Expression data from adult liver of offspring derived from cultured spermatogonia (GS cells) cryopreserved for a 6 months on the International Space Station (ISS) or on Earth.
Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and tranplanted into the seminifelous tubules of infertile W mice. Recipients were housed with wild-type C57BL/6 females, and livers of F1 offspring were analyzed for gene expression. Overall design: In this dataset, we include the expression data obtained from livers of 10 week old offspring derived from GS cells cryopreserved on the ISS or on Earth.   As a control, we also include the expression data from the livers of wild-type BDF1 control mice. Each group contains 6 biological replicates.The liver of adult offspring were homogenised, and total RNA samples were extracted with TRIzol reagent (Invitrogen) and purified using the RNeasy cleanup system (QIAGEN, Valencia, CA). The RNAseq libraries were generated using the  Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA). Sequencing was performed on an Illumina NextSeq 2000 platform with the paired-end mode.