Dynamic changes of macrophage polarization during Salmonella infection in zebrafish
To investigate the polarization status of macrophages during salmonella persistent infection we established a model of Salmonella enterica serovar Thyphimurium infection in the zebrafish larva in which salmonella survive long term inside the host. In this model, during initial stages of the infection, macrophages are recruited to the infection site and express tnfa. By contrast, in the late stages of the infection, macrophages form clusters at the infection site and do not express tnfa. The objective is to characterize the transcriptional profile of macrophages in early stage of infection (4 hours post-infection) and late stage of infection (4 days post-infection). Overall design: Comparative gene expression profiling analysis of RNA-seq data for macrophages from Salmonella infected larvae at 4 hours post-infection (4 replicates) and 4 days post-infection (4 replicates). Controls are macrophages from non-infected larvae at 4 hours post-infection (4 replicates) and 4 days post-infection (3 replicates).