ChIP-seq assay for BRG1 in human SUP-B15 and Nalm-6 cell lines
Abstract: BRG1 is highly expressed in adult B-cells acute lymphoblastic leukemia and is associated with poor prognosis. High expression of BRG1 promotes the proliferation and resistance to apoptosis of B-ALL cells. BRG1 exerts these functions mainly through activation of the PI3K/AKT signaling pathway. In order to understand the specific regulatory mechanism, we used ChIP-seq to detect the DNA fragments bound by BRG1 in SUP-B15 and Nalm-6 cell lines. Overall design: ChIP-seq assay for BRG1 in two human B-ALL cell lines (SUP-B15 and Nalm-6 cells)