Single-cell RNA-sequencing of mouse embryonic fibroblasts, ESCs, and control and Hic2 overexpressing intermediate reprogramming cells
Nanog-GFP MEFs were reprogrammed using the piggyBac MKOS reprogramming system with overexpression of genes of interest (BFP as a control, or Hic2). Cells were collected on days 2, 4, 6 and 9, alongside Nanog-GFP MEFs and Nanog-GFP mESCs.