RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic and fructo-oligosaccharides
Purpose:Our data significantly advance understanding of Probiotics and Fructooligosaccharide regulatory mechanism  in  ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Probiotics and Fructooligosaccharide Results: A total of 164,629,826 and 149,883,266  raw reads were generated from the CT and MP_ FOS, respectively . After removing the interference data, about 164,028,872 and 149,364,852 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after  given probiotics Overall design: Control group (CT), birds were fed a corn-soybean basal diet , Probiotics and Fructooligosaccharide group (MP_ FOS), birds were given a basal diet supplemented with  2 mL of probiotics per litre of water and 1 mL of  Fructooligosaccharide per litre of water