Transcriptional analysis of dendritic cells upon TLR stimulation reveals an immunosuppressive profile [PLF scRNA-seq]
Dendritic cells (DCs) simultaneously prime and dampen the immune response. The transcriptional program that regulates immunosuppression in DCs is currently unknown. Here we identified ETS homologous factor as a key transcription factor that regulates DC immunosuppression. Overall design: Mice were immunized intraperitoneally (i.p.)  with or without 10 µgTLR stimulation in 100 µL PBS, peritoneal cavity cells were obtained by peritoneal cavity lavage twice with 5 mL of ice-cold PBS. They were collected 6 hrs after injection for cDCs (live MHCII+ CD11c+) FACS-sorting for scRNA-seq library preparation.