Invitro RNA sequencing of Xanthomonas T6SS mutant
The project was to identify the differentially expressed genes between wild type and T6SS gene mutant of Xanthomonas perforans strain AL65 under invitro condition. The wild type and the mutant strains were grown in XVM2 medium. Total RNA was isolated at two different time points, the mRNA library was prepared and subjected to next generation sequencing.