Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
To investigate how stage-specific activation of canonical Wnt signaling can induce the differentiation of atrioventricular canal cardiomyocytes,We employed single-cell RNA sequencing and ChIP-seq for H3K27ac to interrogate hPSC-derived cardiac cells. Overall design: hPSC-derived cardiac cells at day 11 and day 15 were analyzed using scRNA seq or ChIP-seq.