Transcriptome of human primary macrophages from healthy controls after long-term myelin phagocytosis
CD14+ monocytes were isolated from the blood of healthy human donors by Ficoll density-gradient centrifugation, magnetic cell sorting and LS column systems. CD14+ monocytes were in vitro differentiated to macrophages by culturing the cells for 7 days in RPMI medium containing 10% FCS, 1% penicillin/streptomycin, 1% glutamine, 1% Fungizone and supplemented with 50 ng/ml human macrophage-colony stimulating factor (M-CSF) in the presence or absence of myelin isolated from brain tissue of 6-8 week-old wild-type C57BL6/J mice. Overall design: Comparative gene expression profiling analysis of human monocyte-derived macrophages differentiated in the absence or presence of myelin from brain tissue of wild-type C57BL6/J mice