Transcriptome analysis in response to symbiotic nitrogen fixation in Lotus japonicus.
To obtain gene expression profile in response to symbiotic nitrogen fixation during root nodule symbiosis in Lotus japonicus, we conducted RNA-seq analysis in non-inoculated, wild-type (WT) and delta NifH rhizobia-inoculated conditions. Whorl shoot tissues of Lotus japonicus MG20 WT plants were collected 11 days after rhizobial inoculation when nitrogen fixation occurred in nodules colonized by WT rhizobia.