Whole exome sequencing of murine Ink4a/Arf knockout NK cells
Whole exome sequencing (WES) was performed to elucidate mutational changes upon the process of transformation and disease initiation. We generated several murine NK cell lines using an Ink4a/Arf KO mouse model. NK cells were harvested from 8-12 week old mice. NK cell lines were then derived either from long-term culture (in vitro generated NK cell lines) or from diseased mice, which developed NK cell leukemia upon transplantation of the in vitro generated cell lines (leukemic NK cell lines). Additional samples of NK cells isolated from the spleen of healthy/untransformed mice were included as controls.