RNA sequencing analysis of in vitro-derived early spinal cord progenitors
human embryonic stem cells (hESCs) were treated with WNT and FGF agonists to generate NMP-like cells (D3) which were then subsequently treated with WNT and high levels of FGF signalling to produce early spinal cord progenitors (D7 of differentiation) Overall design: Gene expression profiling utilised total RNA extracted from hECS cells (N=3); D3 axial progenitors (N=3); D7 spinal cord progenitors (N=3)