Rhizopus sp. genome sequencing and assembly
This project contains Illumina Whole Genome Sequencing (WGS) data. Genomic DNA libraries were prepared from isolated DNA using the Illumina DNA Prep kit (Illumina, USA, #20060059), following the manufacturer's instructions, including fragmentation, index/barcode addition, amplification, and purification. Library size distribution was assessed with the 2100 Bioanalyzer (Agilent, USA, #G2939BA). Sequencing was performed on the Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads, with approximately 5 million reads per sample. Raw reads were quality-checked using FASTQC, evaluating read count, quality scores, GC content, k-mer distribution, and potential adapter contamination. Low-quality bases and adapter/index sequences were trimmed using Trimmomatic (v0.39). De novo assembly was conducted with Shovill, which utilizes the SPAdes assembler, and resulting contigs were compared against known genomic sequences using NCBI BLAST. Taxonomic identification was based on sequence similarity and coverage metrics, resulting in the identification of the organism as Rhizopus microsporus. This dataset provides high-quality WGS reads suitable for genomic analyses, including assembly, annotation, and comparative genomics.