16S rRNA gene amplicon sequencing of gut microbiota in patients with anorexia nervosa and healthy controls
This study aimed to investigate the differences in the composition and diversity of gut microbiota between patients with anorexia nervosa (AN) and healthy individuals. We collected fecal samples from 30 AN patients and 30 age-matched healthy controls. The V3-V4 region of the 16S ribosomal RNA gene was amplified and sequenced using the Illumina MiSeq platform. The resulting sequences were analyzed to compare the microbial community structure between the two groups. This research may provide insights into the role of the gut-brain axis in the pathophysiology of anorexia nervosa.