Transcriptome sequencing of testes in tetraploid fish and diploid red crucian carp
Tetraploid fish are valuable diploid sperm donors and vertebrate models, and their reproductive characteristics have been widely attracted. In previous study, we obtained a new tetraploid fish (4nNT, 4n = 200, AAAB) derived from a cross between female autotetraploid fish (4nAU, 4n = 200, AAAA) and male allotetraploid fish (4nAL, 4n = 200, AABB). Compared with the original parent red crucian carp (Carassius auratus var. red, RCC, 2n = 100, AA), a high proportion of abnormal sperm was observed in the semen of 4nNT. In order to gain further insight into the reproductive potential and fertility mechanisms of the 4nNT, a comparative analysis was conducted between 4nNT and RCC in terms of testis transcriptome.