Exploring role of miRNA in plant fungal interaction
miRNA is a 21-bp, non-coding RNA that regulates gene expression at the transcription level. These miRNAs have been identified in different fungi. In our study, we have worked on identifying miRNAs in Fusarium oxysporum f. sp. lycopersici and their targets in pathogen only, involved in its growth and development. We have completed sRNA sequencing of Fol mycelial RNA sample grown at 20 and 28 degree Celsius . The miRNA expressing at two different temperatures were amplified with stem-loop PCR and its expression pattern was studied. Subsequently, we also completed target validation of miRNA with RLM-RACE.