Arthrobotrys flagrans Epigenomics
This study aims to elucidate the molecular mechanisms by which four transcription factors regulate key developmental processes in the nematode-trapping fungus Arthrobotrys flagrans. Specifically, we investigate the roles of WC2, VelB, and Ste12 in regulating the formation of chlamydospores, that are crucial for the commercial development of this fungus as a biocontrol agent against pathogenic nematodes. Additionally, we examine the function of Smp1 in controlling trap morphogenesis, a critical step for capturing and killing nematodes. To achieve a genome-wide understanding of their regulatory networks, we performed DNA affinity purification sequencing (DAP-seq) to identify the direct DNA-binding targets of WC2, VelB, Ste12, and Smp1. The resulting raw sequencing data will provide a comprehensive transcription factor binding landscape, enabling the reconstruction of downstream gene regulatory cascades. Elucidating these networks will not only advance our fundamental knowledge of fungal development and predation but also provide a theoretical basis for engineering stable, high-yield chlamydospore-based formulations and enhancing the nematode-trapping efficiency of this promising biocontrol fungus.