The CDK9 Complex Regulates Transcription in Human Nucleus Pulposus Cells
CDK9 interacts with its regulatory partner cyclin T, forming the positive transcription elongation factor-B (P-TEFb) that regulates elongation by phosphorylating RNA polymerase II. However, the function of CDK9 in human nucleus pulposus cells has not been fully explored. Here, we demonstrate the enrichment of CDK9 at various gene promoters in human nucleus pulposus cells and compare it with a negative control (IgG) to identify genes that are transcriptionally regulated by CDK9. Overall design: We collected three human nucleus pulposus samples and primary nucleus pulposus cells(NPCs) were cultured. Then, NPCs were harvested and  incubated the anti-CDK9 antibody (Proteintech), Goat anti-Rabbit IgG secondary antibody (1:5000, Fudebio, Hangzhou, China), and Hyperactive PG-TN5 / PA-TN5 Transposon and then fragmented.