Mus musculus Raw sequence reads
In order to explore the regulatory role of the RBP we predicted on alternative splicing, an experiment was carried out. Using effective siRNAs, Eif4a3 was selected as an example and its expression was decreased in mouse zygotes via microinjection. Blastocysts were collected on the fifth day, with 3 samples from each of the Negative Control group (NC) and the Eif4a3-knockdown (Eif4a3-KD) group, each sample containing 5 embryos. The embryos were then placed in SMART lysate and sent to LC-Bio Technologies (Hangzhou) Co., Ltd for Smart-seq2 sequencing, which was conducted using the Illumina NovaseqTM 6000 150PE platform.