eIF5Ad RNA-seq
Ribosome pausing coordinates co-translational folding, chaperone engagement and organelle targeting, yet prolonged stalls can trigger Ribosome Quality Control (RQC) and abort translation. What dictates whether a paused ribosome resumes elongation or is committed to termination remains unresolved. By depleting eIF5A to globally slow down elongation we here examine the consequences of pervasive ribosome stalling in eukaryotic cells.