transcriptome studies of ntslah3 mutant under salt stress
To investigate the transcriptomic response to salt stress mediated by NtSLAH3, we generated ntslah3 mutants using the CRISPR/Cas9 system. Both mutant and wild-type (WT) plants were subjected to a 12-hour NaCl treatment, followed by RNA-Seq analysis to identify differentially expressed genes and elucidate the regulatory role of NtSLAH3 in salt tolerance.