GLORI mapping of m6A sites in human primary CD8? T cells under activation and METTL3 inhibition
Glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) was applied to human primary CD8? T cells subjected to in vitro activation with aCD3/aCD28 beads. To identify N6-methyladenosine (m6A) sites at single-nucleotide resolution and to test their dependence on METTL3, we generated GLORI libraries from non-activated (NoAct) CD8? T cells, Day1-activated T cells treated for 24 hours with a competitive METTL3 inhibitor (4 µM), and Day5-activated T cells treated with the same inhibitor for 5 days. These datasets provide high-resolution m6A maps and allow comparison of m6A regulation in the presence or absence of METTL3 activity during distinct states of CD8? T cell activation.