Gene expression profile at single cell level  of Drug-tolerant persister cells induced by targeted therapy in lung adenocarcinoma.
Drug-tolerant persister (DTP) stage is viewed as a major source of non-genetic drug resistance. We use single cell RNA seq to reveal the transcriptomic heterogeniety of DTP stage Overall design: PC9 cells were treated with 150nM Osimertinib for 4 days or 11 days to induce DTP stage. NCI-H358 cells were treated with 500nM Sotorasib for 8 days to induce DTP stage. All DTP cells (3 samples) and untreated control cells (2 samples) were collected, undergo cell hashing with barcoded antibodies and subsequently pooled in one sc-RNA seq run. *************************************************************** Raw files for human/patient samples are available in dbGaP (https://www.ncbi.nlm.nih.gov/gap/) phs004458.v1.p1 for controlled access to the personally identifiable sequence data. ***************************************************************