DEAD-box RNA helicases regulate RNA silencing and plant immunity and are manipulated by RNA silencing suppressor of powdery mildew fungus
Total RNA was extracted from WT barley GP and transgenic lines RH37i-12, RH52i-18, and RH37i-RH52i-4 (obtained by hybridizing RH37i and RH52i) with three biological replicates for each genotype. Using TRIzol reagent (Invitrogen, 15596-026), 1 ug of total RNA per sample was used to construct sRNA libraries for sequencing on an MGISEQ-2000 platform equipped with the single-end 50 bp (SE50) module.Total RNA was extracted from WT barley GP and transgenic line RH37i-RH52i-4 (obtained by hybridizing RH37i and RH52i) with three biological replicates for each genotype. Using TRIzol reagent (Invitrogen, 15596-026), 1 ug of total RNA per sample was used to construct RNA libraries for sequencing on an MGISEQ-2000 platform equipped with the pair-end 150 bp (PE150) module.