RNA sequencing of human and mouse cells with either endogenous or exogenous overexpression of APOBEC2 cytidine deaminase-like protein
To identify transcriptome-wide effects of the APOBEC2 cytidine deaminase-like protein, human HEK-293T and mouse C2C12 cells were transiently transfected with either an empty-vector control construct or an expression construct for the species's APOBEC2 gene. Separately, for endogenous APOBEC2 overexpression, parental human LHCNM2 and mouse C2C12 cells were differentiated by reducing the amount of serum in cell culture medium. RNA was extracted from parental and differentiated cells. Directional RNA sequencing libraries were prepared from total RNA extracted from cells (biological triplicates) and the libraries were subjected to RNA sequencing. Comparison of transcriptome sequences did not reveal any unknown RNA editing activity of human APOBEC2 or mouse APOBEC2 (unpublished work, Santosh Patnaik, Roswell Park Comprehensive Cancer Center, Buffalo, USA).