Environmental DNA metabarcoding results from water collected in the Tennessee River watershed in northern Alabama
Environmental DNA (eDNA) water samples were collected from 50 stream and river sites of the Tennessee River Basin in northern Alabama, United States of America. We extracted eDNA from these samples, and sequenced the eDNA using vertebrate-specific 12S rRNA primers. We used our results to calculate an eDNA-IBI and compared our results to a previous fish-IBI.