Complete genome sequences of Providencia bacteriophages PibeRecoleta, Stilesk and PatoteraRojo
Providencia is a genus of gram-negative bacteria within the order Enterobacterales, closely related to Proteus and Morganella. While ubiquitous in the environment some species of Providencia, such as P rettgeri and P stuartii, are considered emerging nosocomial pathogens and have been implicated in urinary tract infection gastrointestinal illness and travelers diarrhea. Given their intrinsic resistance to many commonly used antibiotics, this study aimed to isolate and sequence bacteriophages targeting a clinical P. rettgeri isolate. Here, we report the complete genome sequence of three novel Providencia phages: PibeRecoleta, Stilesk and PatoteraRojo, which were isolated against a clinical P. rettgeri strain sourced from a patient in a metropolitan hospital in Victoria Australia. The three phages contain dsDNA genomes between 60.7-60.9 kb in size and are predicted to encode between 72-73 proteins. These three new phages, which share high genomic similarity to two other Providencia phages previously isolated on P stuartii, serve as important resources in our understanding about Providencia bacteriophages and the potential for future phage-based biotherapies.