Microbiota composition and its metabolites in metastatic colorectal cancer patients before and after chemotherapy by metagenomic sequencing and NMR spectroscopy
Despite advances in the diagnosis of colorectal cancer (CRC) recent years, CRC associated with poor prognosis still. There is growing evidence that microbial dysbiosis and the metabolites of gut microbiota contribute to the development of CRC. Thus, analysing stool samples not only provide an excellent opportunity to investigate possible biomarkers for diagnosis, but also for follow-up and treatment approaches. The importance of this study based on relatively few the data on the microbiota composition taken from the patient before chemotherapy (bc), which is the most common form of treatment, and the data on the composition of the microbiota after the patient receives chemotherapy (ac). As forgotten organ in human health and disease understanding microbial composition changes can give us unique notifications releated with cancer. This purpose, a total of 50 fecal samples; 25 fecal samples before chemotherapy, 25 fecal samples after chemotherapy,and 10 fecal samples from healty controls were collected. Using 16S rRNA, metagenomic analysis were performed.