10-Hydroxy-2-decenoic Acid Inhibits Colorectal Cancer via Regulation of the Wnt/beta-Catenin Signaling Pathway
The present study aimed to systematically evaluate the inhibitory effects of 10-HDA on colorectal cancer both in vitro and in vivo and to explore its potential mechanisms through transcriptomic analysis of xenograft tumors. In vitro, the inhibitory effects of 10-HDA on colorectal cancer cells were assessed using cell viability, colony formation, and migration assays. In vivo, a mouse xenograft tumor model was established to validate the antitumor efficacy of 10-HDA. Transcriptome sequencing, western blotting, and immunohistochemical analyses were performed to investigate alterations in relevant signaling pathways. The results showed that 10-HDA significantly reduced the viability of colorectal cancer cells and suppressed colony formation and cell migration, demonstrating notable antitumor activity in vitro. Consistently, in vivo experiments further confirmed that 10-HDA effectively inhibited the growth of xenograft tumors in mice. Transcriptomic analysis revealed that the Wnt/beta-catenin signaling pathway may play a critical role in the antitumor effects mediated by 10-HDA. Furthermore, changes in key molecules involved in this pathway were validated by western blotting and immunohistochemical analyses.In conclusion, this study demonstrates that 10-HDA exerts significant inhibitory effects on colorectal cancer, potentially through the regulation of the Wnt/beta-catenin signaling pathway. These findings provide experimental evidence and theoretical support for the potential application of 10-HDA as an adjuvant therapeutic agent for colorectal cancer.