Identification of a New DRB1*15 allele
Here we describe a novel DRB1*15 allele, identified in a a french  volunteer bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following:  A*66:02 ,*68:02 B*07:02 ,*53:01 C*04:01 ,*07:02  DRB1*07:01 ,*15:New DRB4*01:01P  DRB5*01:01 DQA1*01:02,  DQA1*03:03 DQB1*02:02, *06:02 DPA1*02:02 ,*03:01 DPB1*01:01 ,*105:01 HLA-DRB1*15 : New allele differs from DRB1*15 :03 by one nucleotide substitution at position 218  of exon 2 (Posion 318 of cDNA) , where  base C is replaced by T without  amino acid change. In fact, the  77th codon  of HLA-DRB1*15:01 (ACC) becomes ACT in this DRB1*15:New, both of them code for Threonine.