Gut microbiota derived deoxycholic acid promotes pancreatic regeneration following acute pancreatitis via Reg3a signaling pathway
Microbial DNA was extracted from fecal samples using a commercial kit. The V3-V4 region of the 16S rRNA gene was amplified using primers 338F and 806R 41, purified, quantified, pooled in equimolar amounts, and sequenced on an Illumina platform (paired-end) following the standard workflow (Majorbio Bio-Pharm Technology). Reads were demultiplexed, quality-filtered with fastp, and merged with FLASH 42,43. Sequences were clustered into OTUs at 97% similarity using UPARSE, and representative sequences were taxonomically assigned using the RDP classifier against the SILVA database (release 138) with a confidence threshold of 0.7 44.