Transcriptomic profile of cord blood B cells and thymic B cell subsets in human neonates
We reported two major thymic B cell subsets (CD21+CD35+ and CD21-CD35-) with different morphology, functions and transcriptome profile. For this purpose, mRNA was extracted from cord blood CD19+ B cells and thymic B cells (CD21+CD35+ and CD21-CD35-) from neonatal donors ranged between 1 day and 4 months and sequenced using Illumina HiSeq4000. Overall design: Examination of transcriptomic profile of cord blood B cells and two subsets of thymic B cells (CD21+CD35+ and CD21-CD35-) in human neonates by RNA-sequencing.