CoCas9, a compact nuclease from the human microbiome for efficient and precise genome editing
Characterization of the small Cas9 nuclease CoCas9 from Collinsella sp. AM34-10. The PAM sequence of CoCas9 was determined through in vitro cleavage of a synthetic DNA oligonucleotide library. Activity of CoCas9 in the mouse retina was determined by deep sequencing. Precision of CoCas9 was assessed by GUIDE-seq together with SpCas9 and SaCas9.