ATAC-sequence in H69AR and H69 celllines
ATAC-seq pipeline was utilized for stringent quality control and statistical analysis of sequencing data, ensuring the reliability of accessibility profiles. Paired-end reads (150 bp) were aligned to the reference genome (human: hg38; mouse: mm10) following a standardized mapping protocol. To detect differentially accessible regions, the DiffBind R package was employed, using statistical modeling to identify significant chromatin accessibility variations between conditions.