Effect of NUTF2 overexpression on gene expression in MCF-7 cells
We generated MCF-7 cells stably expressing an exogenous NUTF2 gene or the empty vector Overall design: We performed gene expression profiling analysis by RNA-seq of the 2 stable cell lines. We also performed CUT&RUN for Estrogen Receptor-alpha and HA-tagged NUTF2 in the stable cell lines after treatment with vehicle, 17beta-estradiol, or 4-hydroxytamoxifen for 1 hour.