Difamilast treatment inhibits the expression of inflammatory mediators in bone marrow-derived basophils (BMBAs) stimulated by IL-3 + IL-33
In this study, we explored gene expression profiles of IL-3+IL-33-stimulated BMBAs treated with difamilast, a highly selective PDE4B inhibitor. We identified that difamilast-treated basophils showed downregulated expression of inflammaory mediators including Il4, Il6 and Il13. Overall design: Bone marrow-derived basophils were stimulated with IL-3+IL-33 or control PBS for 4hrs in the presence of difamilast or vehicle DMSO, and were subjected to bulk RNA-seq analysis.