Genome reorganization and its functional impact during breast cancer progression [Micro-C]
We used Micro-C to explore conformational changes in the cancer genome, using the MCF10 cell line as a model for breast cancer progression. We have generated 5kb-resolution maps in normal (MCF10A), pre-malignant (MCF10AT1), and metastatic (MCF10CA1a) breast cancer cell lines, and aim to identify differential enhancer-promoter interactions between the different stages of cancer progression that may contribute to oncogenesis. Overall design: Micro-C in MCF10A, MCF10AT1, and MCF10CA1a cell lines (2 biological replicates per cell line, 4 technical replicates per biological rep). Micro-C was conducted by Cantata Bio.