CREEPY: CRISPR-mediated editing of synthetic episomes in yeast - Sequencing Raw Data
Sequencing Raw Data used in our CREEPY study. In this project, we describe CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. We demonstrate that CRISPR editing of circular episomes presents unique challenges compared to modifying native yeast chromosomes. We optimize CREEPY for efficient and precise multiplex editing of >100 kb yeast episomes, providing an expanded toolkit for synthetic genomics.