Identification of PRMT1 as a suppressor of MHC-I and anti-tumour immunity
To understand the mechanisms by which Prmt1 knockout or inhibition sensitizes B16F10 to T cell mediated killing. Overall design: B16F10 cells were pre-treated with Prmt1-inhibitor GSK3368715 (2.5 µM) for 2 days. Afterwards, untreated wildtype (controls), Prmt1-inhibitor treated or Prmt1-/- B16F10 were cultured for one more day in the presence or absence of mouse IFN? (1 ng/ml). There are three independent replicates for each condition. Total RNA was then extracted, quantitated with 4200 Tapestation and finally, 1 µg of total RNA per sample was subjected to NGS library preparation (TruSeq RNA). NGS libraries were pooled and sequenced using a P2 100-cycle Illumina sequencing kit on the NextSeq 2000 sequencing system with paired-end 66bp reads.