Mus musculus Raw sequence reads
living T cells were collected, and nuclei were extracted. The nuclei suspensions were incubated in a Transposition Mix containing Tn5 Transposase. Upon entering the nucleus, the transposase preferentially fragments the DNA in open regions of the chromatin. Simultaneously, adapter sequences are added to the ends of the DNA fragments. After transposition, immediately purify the products using Beckman SPRIselect Kit. The DNA fragments were then used for PCR amplification and purification to construct a sequencing library. After testing quality, the library was sequenced using Illumina NovaSeq XPlus.