Perform CHIP-seq analysis by Pax5 antibodies to investigate the changes in Pax5 transcriptional regulation between control Ramos cells (Ramos), the lentivirus vector infected Ramos cells (Ramos-Lv-Vector) and Ramos-Lv-ZEBRA.
EBV immediate early protein ZEBRA was corroborated to interact with Pax5 which controls the fate of B cells. Ramos cells were infected with ZEBRA-expression lentivirus and positively infected cells were sorted, which were named Ramos-Lv-ZEBRA. In order to investigate the changes in Pax5 transcriptional regulation among the control Ramos cells (Ramos), the lentivirus vector infected Ramos cells (Ramos-Lv-Vector) and Ramos-Lv-ZEBRA, CHIP-seq analysis was performed using Pax5 antibodies.