Revealing the underlying regulatory mechanism of LINC00313 in Osimertinib-resistant NSCLC cells by ceRNA network analysis
In this study, RNA-sequencing technology was employed to identify the differentially expressed lncRNAs (DE-lncRNAs) and mRNAs (DE-mRNAs) between H1975 and H1975OR. Based on the starbase 2.0 database, the potential targeting relationships between DE-lncRNAs and DE-mRNAs were predicted and identified to construct the ceRNA networks. Subsequently, functional and pathway enrichment analysis were carried for target DE-mRNAs to obtain functional pathways associated with Osimertinib resistance and potential drug resistance-related DE-mRNAs. Besides, the expression of LINC00313 and COL1A1 was validated by q-Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Finally, the activation of PI3K/Akt pathway was proved by immunohistochemistry staining. Overall design: Osimertinib resistant H1975 cells (H1975OR); osimertinib-sensitive H1975 cells (H1975)