Whole genome sequencing of canine osteosarcoma samples collected from COTC021/022/026/030 clinical trial
Tumor DNA samples that passed QA/QC (260/280 ratio > 1.8; >200 ng total DNA) were converted into libraries using TruSeq Nano DNA library prep, pooled and sequenced on NovaSeq 6000S4 flowcells in paired-end sequencing mode. The samples were mapped, and variants were called using Dragen. Percent total mapping against reference canine genome (CanFam4, GSD_1.0) was about 99% and uniquely mapped reads were above 70%. Library complexity (i.e. percentage of unique reads) was determined by measuring the percentage of unique fragments in the mapped reads using MarkDuplicate utility. Coverage statistics were also measured using Dragen. The mapped sequencing depth coverage (after alignment and marking duplicates) was between 22x-133x .