Microbial composition of digesta and mucus specimens from the African catfish (Clarias gariepinus)
Mid and hind intestinal contents were collected from the adult, intensively reared African catfish, and, based on visual inspection, samples were designated as F (digesta) or M (mucus). In case no clear assignment could be provided, samples were designated MF. DNA was extracted, and a 16S library was prepared using 341F (5'-CCTACGGGNGGCWGCAG-3')-785R (5'-GACTACHVGGGTATCTAATCC-3') primer pair. Sequencing was done using the MiSeq platform (paired-end, 2 x 250 bp).