Impact of Electric Potential on the Transcriptome of Paracoccus denitrificans in denitrification Conditions
This study aimed to evaluate the transcriptomic response of Paracoccus denitrificans PD1222 exposed to an applied electric potential of +200 mV under aerobic denitrification conditions. RNA-seq analysis was conducted on five biological samples: three independent replicates under 200 mV and two control samples at 0 mV. Differential gene expression changes revealed genes associated with amino acid metabolism, carbohydrate metabolism, membrane transport, and energy metabolism. Overall design: Triplicate cultures of P. denitrificans PD1222 were grown anaerobically in the presence of 7.5 mM nitrate and 3.5 mM succinate as electron donor and acceptor, respectively. Samples were exposed to 0 mV (control) or +200 mV (treatment) for 6 hours.