Twist-ONT: Combining Nanopore Sequencing with the Twist Comprehensive Viral Research Panel
Twist-ONT is a novel protocol for combining the Twist Comprehensive Viral Research Panel (CVRP) with Oxford Nanopore Technologies (ONT) sequencing. During the study we performed virome sequencing of 9 different nasopharyngeal/throat swab samples, 1 throat swab sample, 6 plasma samples, 1 sample of diluted cultured virus, 2 pools of combined samples, and 2 negative controls. The Twist CVRP is a probe-based hybridization enrichment method for whole genome sequencing of all known human and animal pathogenic viruses.