Whole transcriptome sequencing of gastric cancer stem cells and non-gastric cancer stem cells
As a subpopulation of tumor cells with stemness characteristics such as self-renewal ability and high oncogenicity, gastric cancer stem cells (GCSCs) are considered to be tumor-initiating cells and are the root cause of cancer recurrence and chemoresistance. Therefore, an in-depth study of the stemness characteristics and maintenance mechanism of GCSCs is of great significance for the treatment of gastric cancer (GC). In this study, GCSCs and non-gastric cancer stem cells (Non-GCSCs) derived from tumor tissues of GC patients were used for whole transcriptome sequencing. The goal of identifying key differentially expressed circRNAs, lncRNAs, miRNAs and mRNAs associated with GCSCs. In conclusion, we mapped the molecular feathers at RNA level closely related to GCSCs and revealed certain ncRNAs with great research potentia based on the whole transcriptome sequencing. Overall design: Whole trascriptome analysis of total RNA data on circRNAs, lncRNAs, miRNAs and mRNAs for gastric cancer stem cells (GCSCs) and non-gastric cancer stem cells (Non-GCSCs).  A total of 6 samples were collected from tumor tissues of GC patients.