Identification of immunity-related genes preferentially regulated by Manduca sexta Spatzle-1, -2, or E. coli peptidoglycan
The total 30 libraries with 3 biological replicates of 10 groups including the non-injection naive as 0 h control, BSA injection control, Spatzle-1A and 2 at 6 hour and 24 hours, M. sexta cell line non-injection control, and E. coli PG injection at 6 hour and 24 hours were constructed by Henry Bellmon Research Center at Oklahoma State University using the KAPA mRNA HyperPrep Kit for Illumina Platforms. The challenged and control libraries were sequenced on Illumina NextSeq 500 platform using High-Output Kit v2.5. FASTQ files of raw-reads were produced and sorted out by barcodes for further analysis.