SATB1 promotion of trophoblast stem cell renewal
Trophoblast stem (TS) cell renewal and differentiation are essential processes in placentation. Here, we have identified the mechanism/targets of chromatin organizer/transcription factor called special AT-rich binding protein 1 (SATB1) action on TS cell renewal by RNA-seq analysis in Rcho-1 TS cells expressing Satb1 shRNAs. Overall design: Rcho-1 TS cells transduced with control shRNAs were used as controls. Cells transduced with two different shRNAs targeting Satb1 were used as treatment. RNA-seq was performed in TS cells at 4 days (acute) and 10 days (chronic) after transduction.