SNP discovery in horse (E. caballus) Y chromosome by pooled long-range PCR product deep-sequencing
In this population based study, long-range PCR products covering ~186 kb of Y chromosome of 3 pools of horses (8 Lipizzan, 9 different domestic breeds and 1 Przewalski) were sequenced using Illumina GA II. Data was aligned to BAC-derived Y chromosome reference sequence tags (submitted to NCBI). Discovered SNPs and indels were used for paternal lineage analysis.