Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [CutnRun 1]
We evaluated tthe necessity of PGE2-Ptger4 signaling in regulating stemness enhancing effects of arachidonic acid (AA) via CutnRun and found that Ptger4 KO organoids failed to cause  global methylation changes in signature genes that are associated with stem cell reprogramming in response to AA or PGE2. Overall design: Cut&Run was performed from Ptger KO intestinal organoids treated with vehicle,  25 µM AA or 50 nM 6,16-dimethyl PGE2 for H3K27ac.