Effects of a Non-Pharmacological Multidomain Intervention in Older Adults
This study aimed to investigate the effects of a multidomain intervention on gene expression profile of older adults with cognitive frailty in Selangor, Malaysia, using  mRNA gene sequencing. The intervention included a combination of dietary changes, exercise, and psychosocial support.  By analyzing gene expression profile in both groups, this study explores how multidomain interventions may influence mrna in older adults. The findings could provide insights into the role of lifestyle factors on the CF in aging populations. Overall design: About 40 samples from two groups of older adult with CF  (IV0=7, IV6=6, IV12=6, C0= 8, C6=7, C12=5) was used for microarray analysis.mRNA from peripheral blood cells was extracted, purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.