RNA-seq analysis on equine primary and immortalized brain cells
In our work, we report the first establishment of post-natal equine brain-derived in vitro systems, including primary equine brain cells (EpBCs) and their immortalized counterparts (EiBCs). EpBCs were isolated from the hippocampal dentate gyrus and successfully expanded, while immortalization via SV40 large T antigen generated stable EiBCs lines with enhanced proliferative capacity and distinct transcriptomic reprogramming characterized by cell cycle activation and partial dedifferentiation.