Homo sapiens whole pancreatic islet mRNA during culture in alternate media
Two islet preparations (from different donors) were subjected to RNA-sequencing. Within each preparation, subsets of islets were treated with 5 or 8 mM glucose, or 8 mM glucose in a specialized, conditioned medium containing Wnt and R-spondin ligands with Rho and ROCK inhibitors (L-WRN+) for 48 h immediately after initial receipt and hand-selection. L-WRN+ promotes proliferation with undue de-differentiation. Polyadenylated mRNA was obtained from 1 mcg of human islet total RNA and 50 nt, single-end sequencing reads were obtained from an Illumina HiSeq 2500.