Plasmid DNA Sequencing and Assembly Using ONT PromethION Platform
This project aims to sequence and analyze plasmid DNA constructs using Oxford Nanopore Technologies PromethION platform. Plasmid DNA was extracted, purified, and prepared for long-read sequencing. High-quality reads were basecalled, assembled de novo, and annotated to identify inserted genes and sequence variants. These data will support synthetic biology and vector validation research.