Lung mesenchymal cells from wild-type mouse lungs
Lung fibroblasts include several subpopulations. We used single cell RNA sequencing (scRNAseq) to analyze the heterogeneity of lung fibroblasts. Overall design: Lung fibroblasts of three wild-type mice (12wks, male) were isolated by FACS using negative selection of lineage markers (CD45, CD31, E-cadherin, CD146, LYVE-1, TER119).