16S rRNA partial gene sequences in rice-associative bacteria
DNA in crown root (CR), unelongated stem (US), and elongated culm (EC) of rice grown in fields, in which chemical fertilizer (CF) or manure compost (MC) was applied, was extracted using DNeasy Plant Mini Kit (Qiagen, Tokyo, Japan). Construction of the metagenomic library targeting the 16S rRNA gene V3 and V4 region and sequencing by a next-generation sequencer MiSeq (Illumina, San Diego, CA, USA) were performed with an NGS service (Macrogen, Tokyo, Japan).