Bacteria-phage coevolution to MPs in the community and species level Metagenome
Community-level response of bacteria-phage to MPs. To investigate the evolutionary response of soil-derived bacteria and viruses to PHA and PET, 1 mL of the bacterial-viral suspension was inoculated into 10 mL of the minimal medium containing each respective carbon source (glucose, PHA, or FDBz). A minimal medium was used for incubation, supplemented with glucose (control), PHA, or FDBz (PET analog) as the sole carbon source, respectively. Species-level response of bacteria-phage to MPs. The E.coli BL21 and lambda phage were co-incubated for 28 days, and subcultured for every two days during the incubation under the substrates of glucose, PHA and FDBz (PET analog).