Multi-Objective LNP Engineering with Artificial Intelligence (MOLEA) enhances tissue-selective mRNA delivery
This project investigates lipid nanoparticle (LNP) engineering for tissue-selective mRNA delivery using an artificial intelligence-guided multi-objective optimization framework (MOLEA). By integrating lipid structure features with cell-type-resolved transfection data, MOLEA enables the identification of ionizable lipids with high delivery efficiency and reduced off-target uptake. Optimized LNPs are further applied for gene editing in target tissues and evaluated using targeted sequencing and functional assays.