Effect of kncoking down CtBP and TRIM28 on gene expreession in MCF-7 cells
Both TRIM28 and CtBP are important transcriptional repressors and always shown high expression in many types of cancers, in our current study, we found TRIM28 interacts with CtBP in breast cancer and normal cells. To elucidate the functions of interaction between TRIM28 and CtBP in breast cancer progression, we performed the RNAseq of knocking down TRIM28, CtBP and Ctrl(NC) genes in MCF-7 cell line. Overall design: MCF-7 cells were transfected with TRIM28, CtBP1/2, Ctrl(NC) siRNA for 2days. RNA was extracted using the RNEasy Plus Micro kit (Qiagen, 74034). RNA-seq and analysis were performed by Novogene. Briefly, samples were sequenced using an Illumina HiSeq, with a 2?×?150?base pair configuration and 20 million paired-end reads was sequenced across nine samples.