RUNX3,a brand new NK cell infiltration biomarker mediates the metastasis of colorectal cancer
Single-cell RNA sequencing (scRNA-seq) technology enables in-depth analysis of gene expression profiles within specific immune cell populations in the TME, providing reliable insights into the molecular characteristics of infiltrating immune cell clusters and greatly facilitating biomarker identification. Overall design: This study integrates scRNA-seq and bulk RNA-seq data to identify potential cancer biomarkers in colorectal cancer, followed by prognostic analysis to screen candidate biomarkers. Ultimately, we identified the RUNX3 molecule as a potential biomarker for colorectal cancer. We subsequently generated RUNX3-/- mice, established tumor models, and performed single-cell sequencing to further investigate the role of Runx3 in NK cell infiltration and its regulatory function in colorectal cancer.