SHROOM3 Deficiency aggravates Adriamycin-induced nephropathy accompanied by Focal Adhesion disassembly and stress fiber disorganization
This RNA-sequencing study investigates the role of SHROOM3 in podocyte function and its contribution to renal pathophysiology. Podocytes are highly specialized epithelial cells critical for glomerular filtration barrier integrity. The study explores how SHROOM3 deficiency affects podocyte cytoskeletal architecture and focal adhesion dynamics in the context of Adriamycin-induced nephropathy, a widely used experimental model of podocyte injury and proteinuric kidney disease.  We performed transcriptome analysis using RNA-sequencing on human podocyte cell lines, comparing Control (n=4) and SHROOM3 knockdown (KD) podocytes (n=4). Our objective was to identify the molecular mechanisms and signaling pathways through which SHROOM3 regulates podocyte cytoskeletal organization, with particular focus on stress fiber formation and focal adhesion assembly/disassembly. The dataset provides comprehensive gene expression profiles revealing how SHROOM3 deficiency exacerbates podocyte injury, disrupts actin cytoskeleton dynamics, and impairs focal adhesion stability during nephropathy progression. Overall design: This RNA-seq dataset includes samples from cultured human podocyte cell lines under two experimental conditions: Control podocytes (n=4) and SHROOM3 knockdown (KD) podocytes (n=4). The study investigates the transcriptomic changes associated with SHROOM3 deficiency in human podocytes to understand its role in podocyte cytoskeletal organization and focal adhesion dynamics.  Each biological replicate (n=4 per condition) represents an independent culture of immortalized human podocytes either expressing normal levels of SHROOM3 (Control) or with stable knockdown of SHROOM3 expression (SHROOM3 KD). All samples were cultured under identical conditions and harvested at the same level of confluence to minimize technical variation. Total RNA was extracted from each sample and subjected to RNA sequencing to compare gene expression profiles between the two groups.