Understanding transcriptional profile of alveolar Type I, Type II and double positive (Type I/II+) cells upon KrasG12D activation in mouse lungs during lung adenocarcinoma.
Previously, we identifed alveolar Type II cells as cell-of-origin for Kras induced lung adenocarcinoma. In the current study, we examined the phenotype of Type II cells after Kras activation and found evidence for proliferation of cells that co-express Type I and Type II markers. Using RNAseq, we are trying to charcterize these different alveolar epithelial cells (Type I, Type II and double positive (Type I/II+)) upon KrasG12D activation during lung adenocarcinoma. Overall design: Comparative gene-expression analysis for single positive Type I cells (Pdpn+, Tdtm-) and Type II cells (Pdpn-, Tdtm+); and double positive cells (Pdpn+, Tdtm+) from Sftpc-CreER; LSL-KrasG12D, LSL-Rosa26-Tdtm mice after 4 weeks of tamoxifen administration.