Effect of different nutrients on rumen microbes in buffalo
We used different nutrients to exlpore their effect on the diversity of rumen microbiota including bacteria, fungi and methangens. Our main objective was to manipulate rumen microbiota to mediate rumen fermentation to enhance production perfromance in water buffaloes. We collected rumen contents after feeding of experimental additives to buffaloes and extracted DNA to sequence 16S-rRNA gene for bacteria and while ITS gene for fungi using Illumina MiSeq plateform.