RNA-sequencing analysis of Nrf2 target genes in human pulmonary arterial smooth muscle cells and endothelial cells
To investigate the genes regulated by Nrf2 in human PASMC and PAEC, Nrf2 was knockdown by using siRNA. 48h after siRNA transfection, cells were collected for RNA-sequencing. Overall design: Each cell type were respectively transfected with one Control siRNA and two Nrf2 siRNAs,each treatment has three replicates.