Transcriptome of isolated mouse peritoneal endothelial cells
To characterize the metabolic profile of peritoneal endothelial cells (ECs) in response to peritoneal dialysis (PD), we performed RNA sequencing of peritoneal ECs isolated from mice treated with PD fluid for 6 weeks (n = 3) and from mice treated with saline for 6 weeks (n = 3). We demonstrated that peritoneal ECs had a hyperglycolytic metabolism in response to PD fluid treatment, which is associated with the development of microvascular alterations and peritoneal dysfunction. Overall design: We isolated peritoneal ECs from mice treated with PD fluid for 6 weeks (n = 3) and from sham mice treated with saline for 6 weeks (n = 3). We then performed RNA sequencing of peritoneal ECs.