ULTRASENSITIVE SEQUENCING (SIMSEN-SEQ) FOR ENVIRONMENTAL DNA APPLICATIONS
The advent of Next-Generation Sequencing (NGS) has revolutionized environmental DNA (eDNA) research, offering unprecedented sensitivity in detecting rare species within vast marine ecosystems. However, conventional NGS methods often fail to accurately capture low-frequency variants, posing risks of imprecise detections. Inspired by clinical research, innovations like SiMSen-Seq (simple multiplexed PCR-based barcoding of DNA for ultrasensitive mutation detection using sequencing) have emerged. SiMSen-Seq utilizes molecular barcoding to track individual DNA templates, correcting polymerase-induced errors and quantification biases.