TGF-ß-Driven Mafb Induces Ovarian Cancer Metastasis and Immune Dysregulation by Competitively Binding WTAP to Weaken the Degradative Modification of IGFBP2
This ChIP-seq dataset examines the genome-wide binding profile of transcription factor MAFB in ovarian cancer cells to investigate its role in tumor progression and immune dysregulation. Using ID8 mouse ovarian cancer cells with MAFB overexpression, we performed chromatin immunoprecipitation followed by high-throughput sequencing to identify MAFB binding sites. Surprisingly, our analysis revealed that IGFBP2, a key mediator of MAFB-driven tumor metastasis and immune modulation, was not a direct transcriptional target of MAFB. The ChIP-seq data was generated using an anti-MAFB antibody, with IgG as control. Peak calling was performed using MACS2 (version 2.2.7.1), and peak annotation was conducted using the ChIPseeker R package. Motif analysis was performed with HOMER (v4.11.1), and differential analysis was conducted with MAnorm. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for transcription factor MAFB in ID8 mouse ovarian cancer cells with MAFB overexpression and control cells, using anti-MAFB antibody and IgG control for immunoprecipitation.