The biological mechanisms related to hub genes in the development of heart failure in dialysis patients were investigated through transcriptome sequencing
Purpose: Heart failure (HF) has a very high prevalence in patients with maintenance hemodialysis (MHD). However, there is still a lack of effective and reliable HF diagnostic markers and therapeutic targets for patients with MHD.  Patients and methods: In this study, we analyzed transcriptome profiles of 28 patients with MHD by high-throughput sequencing. Overall design: Sample preparation and processing  Peripheral blood mononuclear cell (PBMC) samples of 15 dialysis patients without heart failure (Normal) and 15 dialysis patients with heart failure (Case) human were collected for mRNA transcriptome sequencing. RNA was isolated and purified from the total samples using TRlzol (invitrogen, CA,USA) according to the manufacturer's protocol. NanoDrop ND-1000 (Wilminton, DE, USA) was used to control the total RNA sender bulk and cleanliness. The fragmented RNA was synthesized into cDNA by reverse transcriptase, and the cDNA was amplified and purified. Building the library and prepared for sequencing on the illumina Novaseq 6000 sequencing platform. After the inferior-quality reads were deleted, the cleanreads were aligned into the human genomic reference (GRCh38_gencode_v33) by HISATI16.