Genome-wide identification of immunity related genes regulated by SnRK1 in Arabidopsis
SNF1-RELATED KINASE 1 (SnRK1) is a master regulators of plant responses to nutrient availability and plays an essential role in regulating multiple plant physiological responses. The goal of this study is to understand an impact of SnRK1 on transcriptional reprogramming during plant immunity in response to cellular energy changes such as sugar limitation in Arabidopsis thaliana. Overall design: Five-day-old wild- type (Col-0) and snrk1a1i/a2 mutant (a dexamethasone inducible SnRK1a1 knock-down in snrk1a2 knock-out mutant) seedlings grown on 1/2 MS agar medium containing 25 mM sucrose were transferred to 1/2 MS liquid medium containing sucrose and DEX, and then treated with or without flg22 for 24 h in the absence of sugar. Total RNA was extracted from whole seedlings. RNA-seq was performed with 4 biological replicates each conditions.