Non-destructive transcriptomics via vesicular export (NTVE)
Transcriptomics is a valuable technique for multiplexed monitoring of cellular states. Using an engineered RNA adapter, NTVE exports cellular transcripts from inducible human and murine reporter cell lines with high concordance to conventional lysate-derived RNA-seq. NTVE allows monitoring of transcriptome changes in response to genetic and chemical perturbations (Interferon gamma; ASCL1 transactivation) in the same cells over time using standard sequencing workflows. Overall design: Samples originate either from cell lysates or were prepped from the supernatant by using a viral RNA preparation kit (see method section https://doi.org/10.1101/2024.11.11.622832)