RNASeq of Mycobacterium tuberculosis treated with qcrB inhibitor and whole-genome sequencing of qcrB inhibitor resistant mutants.
This study investigates the mechanism of action of a new class of qcrB inhibitors against Mycobacterium tuberculosis (H37Rv). The goal is to determine the transcriptional changes resulting from the inhibition of the cytochrome bc1 complex (qcrB) and to compare these changes with the known qcrB inhibitor Q203. In addition, spontaneous resistant mutants generated by the inhibitor were isolated and underwent whole-genome sequencing to identify resistance-conferring variants. These data can provide insights into the mechanisms of action of qcrB inhibitors and serve as a valuable resource for the development of new anti-tuberculosis therapies targeting the respiratory chain.