AAPBs in polar plant tissues
Here, we examined the occurrence of plant-associated aerobic anoxygenic phototrophic bacteria (AAPB) across polar regions. Recently found in polar soils and cold-climate plants, AAPBs are photoheterotrophs that rely on environmental organic carbon but capture solar energy via anoxygenic photosynthesis. We revealed the abundance of AAPBs by extracting bacteria from plant tissues and imaging the colonies with bacteriochlorophyll-based near-infrared fluorescence. The taxonomic distribution of AAPBs was determined via 16S rRNA gene analysis. From the northern hemisphere, we describe AAPBs from the leaf endo- and phyllospheres of numerous sub- and high-arctic plant species in Greenland, Svalbard, and Northern Finland. In the southern hemisphere, we focused on AAPBs in the root and leaf endospheres and the phyllospheres of Deschampsia antarctica in Chilean Patagonia and maritime Antarctica. Additionally, we describe AAPB from the tissues of several other plant species in Patagonia. We found AAPBs commonly associated with the sampled plant species across both hemispheres. A diversity of Alphaproteobacteria was found to contain the AAP-capability: at all sampling sites, Sphingomonas was the most abundant taxon (up to 60%), while Methylobacteria made up a notable proportion of sub-arctic and sub-antarctic AAPB samples (up to 32%). In contrast with previous studies describing Methylobacteria frequently in various plant communities, AAP-containing Methylobacteria were virtually absent from our high-latitude sites. With diverse AAPB taxa found ubiquitously across polar regions and plant tissues, our results call attention to the potential ecological significance between AAPBs and their plant hosts.