Transcriptome analysis of Arabidopsis thaliana roots, H2O2 and Rose Bengal [20180920_173313_H2O2_RB]
In order to better understand the context of root water stress and its relationship to other stresses, I undertook an evaluation of osmotic water stress by transcriptome analyses. Whole transcriptome MARSeq (Diego Adhemar Jaitin, 2017) analysis can enable us to study the changes in gene expression (changes in transcript prevalence) in depth under different treatments. It is a cost effective method to develop transcriptional activity. It uses relatively small amounts of RNA and reads sequences from the 3'-polyA containing end. This method can reveal underlying trends that can help us understand the less obvious processes happening in our system. To carry this out I processed whole transcriptome data separately from WT seedling roots exposed to 5 mM H2O2 or 10  µM RB for 0, 1, 2, 3 h or  5 mM H2O2 or 10  µM for 2h . Overall design: WT Arabidopsis thaliana seedling roots were exposed to 5 mM H2O2 or 10  µM RB for 0, 1, 2, 3 h or  5 mM H2O2 and 10  µM for 2h ., and root RNA was extracted sequenced using the MARSeq protocol.