Modeling NRF2-Driven Anti-inflammatory, Tumor-promoting Macrophages In Vitro
To determine whether NRF2-mediated anti-inflammatory differentiation and its downstream consequences on tumor immunology are intrinsic macrophage processes, we conducted a series of in vitro studies. First, we performed scRNA-seq studies of WT, Keap1 KO, and Nrf2 KO BMDMs after short-term culture with GM-CSF for 5 days to capture a broad spectrum of differentiation and polarization states across the macrophage lineage trajectory. Overall design: scRNA-seq study of WT, Keap1 KO, and Nrf2 KO BMDMs after short-term culture with GM-CSF for 5 days