seawater, Boothbay Harbor, ME Genome sequencing and assembly
In this study, we compartmentalized a coastal seawater sample into picoliter-sized semipermeable capsules for in-capsule amplification and barcoding of DNA from individual particles. We obtained genomic sequences of 2,037 particles from a 250 nanoliter sample, capturing a broad range of cellular and extracellular entities. To assess the efficacy of this capsule-based method for genome recovery, we compared it to two other methods: metagenomics and single-amplified genomes (SAGs), which we generated from the same seawater sample.