Homo sapiens Transcriptome or Gene expression
This project involves transcriptome profiling of human dermal fibroblasts transfected with tRF-34 mimics or negative control (NC). Total RNA was extracted, and RNA-seq libraries were constructed and sequenced on the Illumina NovaSeq X Plus platform. Raw reads were quality-controlled, filtered, and mapped to the human reference genome (GRCh38) using HISAT2. Gene expression levels were quantified in FPKM, and differentially expressed genes were identified using DESeq2 (|log2FC| >= 1, FDR < 0.05). The study aims to investigate gene expression changes associated with tRF-34 regulation in the context of skin photoaging.