Gene expression profile at single level in tumor draining lymph nodes in wild type (DHO001) versus ATG5LEC-KO mice (DHO002)
We used scSEQ analysis to assess differences in the local tumor draining lymph node microenviroment, both on endothelial cells and immune cells Overall design: 13 lymph nodes from wild type mice and 9 from ATG5LEC-KO mice were isolated and digested, endothelial cells (viable CD31+ Ter1119- CD45-) and immune cells (viable CD45+ Ter119- CD31-) were sorted and pooled for further sequencing analysis