Effects of cold stress changes on subchondral bone and cartilage of tibial  knee joint in rats and molecule related to hypothermia and arthritis
Forty eight-week-old male SD-rats were randomly divided into four groups:  control group (4 weeks), hypothermia group (4 weeks), control group (8 weeks) and hypothermia group (8 weeks). The hypothermia group was fed at 4±2?, and the normal group was fed at 25±2?. After 4 weeks and 8 weeks, Micro-CT and saffron solid green staining were performed to observe the conditions of subchondral bone and cartilage. At 4 weeks, rat subchondral bone tissue was cut for second generation high-throughput sequencing, differential gene screening and gene enrichment analysis, and PCR validation. Overall design: To find out which molecules play a role in hypothermia and arthritis,  we performed high-throughput sequencing of subchondral bone  in rats at low and normal temperatures, followed by PCR validation