In-vitro study of differential transcriptomic analysis in indigenous (Gaddi dogs) versus exotic Labrador breed
The work has been aimed at studying the differential transcriptome (mRNA and miRNA) expression profile through in vitro bacterial and viral simulated infection models (in Gaddi dogs and Labrador breed). Four experimental groups have been studied by subjecting the PBMCs of healthy dogs to three TLR-ligands (Poly I:C, LPS, and CpG) individually and compared with that of the Control group (No treatment). The differential transcriptomic (mRNA and miRNA) profiles have been studied to explore the systems biology through analyzing the pathways associated.