A gut commensal protist protects against virus-mediated loss of oral tolerance
To better understand how Tritrichomonas arnold colonization impacts the reovirus-mediated proinflammatory response in dietary antigen-presenting  dendtric cells (cDC1), we examined the transcriptional profile of mesenteric lymph node-derived cDC1. For RNA-sequencing on CD103+ CD11b- CD8a+ DCs, 1,000 CD103+ CD11b- CD8a+ DCs were FACS sorted into a 96-well plate Overall design: Wild-type mice were inoculated perorally with 10^6 Tritrichomonas arnold or PBS control for 12 days followed by peroral inoculation with 10^9 PFU of reovirus strain T1L or PBS control for 48 hours. Smart-seq RNA-sequencing of MHC-II+ CD11c+ CD103+ CD11b- CD8a+ mLN DCs (PBS, n =4), (T1L, n = 3), (T1L+T. arnold, n =4), (T. arnold, n = 3).