Coal-Driven Anaerobic Microbial Diversity through 16S rRNA Sequencing
We identified three dominant bacterial phyla Pseudomonadota, Bacillota, and Actinomycetota across various stages of anaerobic digestion. Pseudomonadota prevalence, ranging from 66% to 84%, underscores its key contribution to methanogenesis, where it degrades coal-derived organic compounds and produces essential substrates for methane-forming archaea.