16S amplicon sequencing of the gut microbiome in patients with ME/CFS, post-COVID and healty controls.
This project contains 16S rRNA gene sequencing data from 163 stool samples collected from patients with post-COVID syndrome (n = 50), chronic fatigue syndrome (n = 76) and healthy controls (n = 37). DNA extraction was performed using a modified version of the Zymo Quick-DNA HMW MagBead kit. Two diferent library preparations were performed: the Illumina 16S Metagenomic Sequencing Library Preparation protocol (V3-V4, 2x300 bp, MiSeq) and the ONT Microbial Amplicon Barcoding Sequencing kit (V1-V9, MinION).