Assessment of the Anti-Tumor Effects of Poly(I:C) Combined with QS-21 on Melanoma Cells(B16F10)
This study explores the synergistic antitumor potential of Poly(I:C) and QS-21 in melanoma treatment. Through in vitro assays and animal models, we evaluated their combined impact on melanoma cell proliferation, apoptosis, and immune response. Our results indicate that Poly(I:C), an immunostimulant, activates the immune system to bolster antitumor activity. QS-21, an immunological adjuvant, enhances Poly(I:C)'s effects, together acting as an in situ vaccine to expose neoantigens and reignite the tumor-immune interaction. This research offers a promising immunotherapeutic approach for melanoma. Overall design: The study involved the treatment of melanoma cells with four different conditions: vehicle (control), Poly(I:C), QS-21, and a combination of Poly(I:C) + QS-21. Each treatment was applied in vitro for a duration of 6 hours. Following the treatment, RNA was extracted from the cells for subsequent sequencing analysis. This design allows for the assessment of the individual effects of Poly(I:C) and QS-21, as well as their combined impact on the melanoma cells' gene expression profile.