Genome sequencing of GB-ls-coga4 line that was derived from a Romanian D. suzukii natural population
During the summer of 2022, we successfully collected D. suzukii individuals from the Botanical Garden of Bucharest (GB). Starting from those individuals, we stabilized the GB-ls-coga4 line, which after a year is expected to nearly meet the criteria of isogenic lines that harbor a homogenous genomic background. In September 2023 we made use of ONT sequencing and employed a MinION device in concert with the Ligation Sequencing Kit V14 methodology (along with the corresponding NEBNext-Companion Module for ONT) and R10.4.1 flow cells. Fast base-calling of POD5 sequencing output was performed with Dorado and FASTQ files were generated.