Transcriptomic response in the brain of Heteropneustes fossilis, under systemic Aeromonas hydrophila infection
In this study, we conducted an in-depth investigation into the molecular mechanisms activated in the brain of Heteropneustes fossilis (Bloch,1794) during systemic Aeromonas hydrophila infection. Using a de novo RNA seq strategy selected due to the absence of a complete reference genome. We generated a high-quality RNA seq assembly that enabled unbiased discovery of transcriptional changes. Our analysis centered specifically on the interplay between neuroendocrine regulation, immune activation, and stress-responsive pathways. By characterizing differential gene expression across these functional domains, we highlight how the brain orchestrates endocrine modulation, neuroendocrine immune crosstalk, and adaptive stress signalling during pathogenic challenge. This integrated view provides deeper insight into the central regulatory roles of the teleost brain in shaping systemic host responses to bacterial infection.