Sequencing Analysis of OL-RA11 Treatment
This study presents RNA sequencing data from a murine skin wound healing model treated with OL-RA11, an autophagy-modulating peptide derived from Odorrana livida. Chronic wounds represent a significant clinical challenge due to limited therapeutic options and poorly understood healing mechanisms. OL-RA11 demonstrated potent efficacy in accelerating wound closure through regulation of the inflammatory microenvironment.Sample types include: mouse skin tissue collected at inflammatory stages of wound healing following OL-RA11 topical treatment. The experimental design compares OL-RA11-treated groups versus vehicle controls to identify transcriptional changes associated with enhanced wound healing.RNA-seq analysis revealed that OL-RA11 primarily regulates autophagy-related pathways, enhancing autophagy flux during wound repair as evidenced by LC3B-II upregulation and p62 reduction. This autophagy enhancement suppresses NF-kappaB signaling, reduces pro-inflammatory cytokine secretion, and promotes M2 macrophage polarization. The PI3K pathway was identified as the key mechanistic target through which OL-RA11 modulates autophagy.Sequencing was performed at Shanghai Majorbio Biotechnology Co. Ltd. This dataset provides valuable resources for understanding the molecular mechanisms of OL-RA11-mediated wound healing and may facilitate the development of novel therapeutic strategies for wound management.This submission supports the manuscript currently under review. Data can be utilized for comparative analysis of wound healing mechanisms, autophagy regulation in tissue repair, and inflammatory microenvironment modulation.