Bacillus subtilis laboratory evolution on tomato roots
The goal was to determine if B. subtilis can adapt to the inhibitory effect of Pseudomonas on roots. Two adaptative laboratory evolution experiments were performed with B. subtilis in mono-association or co-cultured with P. fluorescens on tomato plant roots. Evolved isolates with various colony morphology and stronger colonization capacity of both tomato plant and A. thaliana roots emerged rapidly from the two evolution experiments. Evolved isolates were sequenced at different cycles of evolution.