RNA-seq analysis of cancer cells treated with LAT1 inhibitor or deprivation of valine or isoleucine
To clarify the transcriptomic response in cancer cells treated with LAT1 inhibitors, focusing on valine and isoleucine depletion, total RNA was extracted from pancreatic cancer MIA PaCa-2 cells cultured for 24 hr in RPMI-1640 media containing LAT1 inhibitor nanvuranlat (30 ÂµM) or lacking valine or isoleucine and analyzed by RNA-seq.