16S rRNA gene sequencing of mice gut microbiota
High-fat diet is more common in modern life, it may cause a series of physiological changes in the body, among which the change of gut microbiota is an important aspect. gut microbiota is related to various physiological functions, and the disruption of its balance may be related to the occurrence and development of various diseases. Polysaccharides are a class of substances with various biological activities, which may have regulatory effects on intestinal flora.16S rRNA gene sequencing has emerged as a powerful tool to study the composition and diversity of gut microbiota. To comprehensively analyze the composition of the gut microbiota in mice. By sequencing the 16S rRNA gene, we aim to identify the different bacterial species present in the gut. Determine the diversity of the gut microbiota. This includes measures such as species richness (the number of different species) and evenness (the relative abundance of each species).Sample collection: Appropriate samples from the gut of mice will be collected following standard procedures to ensure the integrity of the microbiota.DNA extraction: High - quality DNA will be extracted from the gut samples. This DNA will contain the 16S rRNA gene of the bacteria present in the gut.16S rRNA gene amplification: Specific primers will be used to amplify the 16S rRNA gene region.Sequencing: The amplified 16S rRNA gene fragments will be sequenced using a suitable sequencing platform.Data analysis: Bioinformatics tools will be employed to analyze the sequencing data. This will include taxonomic classification of the bacteria based on the 16S rRNA gene sequences, and calculation of diversity indices.