Whole genome sequencing of human enteroviruses from clinical samples by direct RNA sequencing
Enteroviruses are small RNA viruses that affect millions of people each year and lead to a large spectrum of symptoms. In this proof-of-principle study, we assessed direct RNA sequencing (DRS) using nanopore sequencing technology for fast whole-genome sequencing of viruses directly from clinical samples. Results of the approach were complemented with those obtained by sequencing the corresponding viral cDNA via Illumina MiSeq sequencing.