Sex determining gene identification in American shad
Our group has recently developed a novel method for indentifying transposon insertion sites using high-throughput sequencing, termed TIP-seq. We applied this method to comparative analysis between male and female Alosa sapidissima (American shad), aiming to develop sex-specific molecular markers and screen for sex determining genes. In this study, we first performed TIP-seq on genomic DNA from both male and female American shad, and identified male-specific transposon insertion sites that enable accurate genetic sex identification of this species. Then, we further conducted whole-genome resequencing of male and female individuals to identify Y-specifc regions and the key sex-determining genes.