U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA 3'-seq]
To understand how U4 snRNP regulates premature cleavage and polyadenylation of pre-mRNAs at the transcriptome wide,  we conducted 3'-seq analysis on control, U1 and U4-AMO treated samples using the Lexogen mRNA 3'-seq kit (Cat. 016.24), which enables accurate quantification of global PAS usage. Overall design: We carried mRNA 3'-seq analysis in NC AMO, U1 and U4 AMO-treated HeLa/HEK-293/Neuro-2A/NIH-3T3 cells