Epigenetic Modulation of microRNA Profiles in Human Skeletal Muscle Myoblast-Derived Extracellular Vesicles Induced by Trichostatin A and Its Osteogenic Implications
This project investigates the epigenetic impact of the histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) on the microRNA (miRNA) profile of extracellular vesicles (EVs) derived from human skeletal muscle myoblasts (HSMMs). The study aims to elucidate the role of TSA-induced hyperacetylation in modulating the miRNA content of HSMM-derived EVs and its subsequent influence on osteogenic differentiation in human bone marrow mesenchymal stem cells (hBMSCs). RNA sequencing was performed to compare the miRNA expression profiles between EVs secreted from TSA-treated and untreated HSMMs (TSA-EVs and UN-EVs, respectively). The dataset includes miRNA sequences that reveal significant differential expression, particularly those miRNAs involved in osteogenesis-related signaling pathways, such as the PI3K-Akt, MAPK, mTOR, and Wnt pathways. The findings from this study suggest that TSA-EVs can mimic the osteogenic benefits of exercise-induced EVs, highlighting the therapeutic potential of HDAC inhibition in osteoporosis treatment. The dataset is expected to contribute to a deeper understanding of the molecular mechanisms underlying HDACi-mediated bone health improvement, offering insights into the clinical application of muscle-derived EVs for bone regeneration.