Characteristics of gut microbiota and metabolites in patients with primary bone malignancies
There is a close relationship between gut microbiota and many intestinal and extraintestinal cancers. To date, research on the relationship between human intestinal microbiota and its metabolites and osteosarcoma is lacking. We hypothesized that patients with distant osteosarcoma exhibit significant alterations in gut microbiota and metabolites, suggesting a potential association between the two. A total of 24 patients with primary bone malignancies and 11 healthy volunteers participated in the experiment. The fecal microbiome of volunteers was analyzed using 16S rRNA gene sequencing to investigate the relationship between the two groups; the differences in diversity, the relative abundance of microbial taxa, the abundance of specific bacteria, and the metabolomic analysis of these samples. Compared with the control group, the diversity of the osteosarcoma group, the abundance of Roseburia, Fusicatenibacter, CAG352, Lachnospira, and Sutterella, and the main metabolites, Flupenthixol and Phytic acid, decreased, while PC increased. These differences suggest a potential interaction between the gut microbiota and its metabolites and osteosarcoma. Clinical significance: To provide a useful reference for the connection between primary bone malignancies and intestinal microbiota.