High-fat Diet Murine Fecal Metagenomics Dataset
Here, we present a longitudinal metagenomic dataset characterizing the impact of seven distinct high-fat diets on the murine gut microbiome over the course of one year. Shotgun metagenomic sequencing was performed on fecal samples collected at multiple timepoints, including baseline, during sustained high-fat feeding, and following dietary reversal to a low-fat control diet after four and nine months. This design enables the evaluation of both acute and long-term effects of dietary fat composition on microbiome structure and functional potential, as well as the persistence or reversibility of diet-induced microbiome changes. The study is integrated with complementary host- and metabolite-level measurements, providing a resource for investigating interactions between diet, the gut microbiome, and host physiology across time and dietary contexts.