A comprehensive analysis of intraspecific variability in yeast rDNA using rDNAmine tool
rDNAmine is a bioinformatic toolkit for capturing and analyzing large repetitive genomic loci in . It enables direct detection of polymorphisms within individual repeats, bypassing alignment-based methods and improving efficiency and scalability in studying repetitive regions. The workflow includes isolation of large-molecule DNA from individual chromosomes to selectively enrich sequencing libraries for extensive genomic regions. Applied to rDNA arrays in Saccharomyces cerevisiae and Candida albicans, rDNAmine reveals species-specific variation in array structure, highlighting its utility for investigating complex repetitive sequences across diverse genomes.