Effect of Alisol B on gene expression in GT1-7 cells
Based on previous experiments showing that Alisol B could regulate GnRH secretion from GT1-7 cells, cellular transcriptome sequencing was used to investigate the potential mechanism by which Alisol B regulates GnRH secretion from GT1-7 cells. Overall design: To investigate the effect of Alisol B on gene expression in GT1-7 cells,  when the cells reached 70-80% confluence, they were starved in serum-free medium for 12 hours. Afterward, they were incubated for 24 hours with or without 0.125µM Alisol B. We then performed gene expression profiling analysis using data obtained from RNA-seq of the Alisol B intervention group and the control group cells with three replicates each. Differential gene expressions analysis of RNA-seq data of the Alisol B intervention group and the control group cells with three replicates each.