Transcriptome and genome-wide analysis of Candida parapsilosis clinical isolates
As Candida parapsilosis is the most common non-albicans agent causing candidemia in neonatal and pediatric populations, recent increases in fluconazole resistance in this species are cause for concern. In order to elucidate mechanisms of triazole resistance present in triazole-resistant Candida parapsilosis isolates, total RNA was isolated from biological triplicate samples of a collection of 40 C. parapsilosis clinical isolates, and, using RNA sequencing and subsequent analysis, transcriptomes were compared. Genome-wide analysis was performed by isolating genomic DNA utilizing a Triton-X, SDS and phenol-chloroform method. DNA concentrations were quantified using both the Qubit Fluorometer and Nanodrop spectrophotometer using the manufacturer protocols. Whole genome libraries were prepared and sequenced on the NovaSeq600 platform with 150bp, paired end reads.