Duncan grapefruit RNA-seq upon treatment with Xcc306 wild type and pthA4 mutant at 12 and 36 hours post infection
We aim to find out genes regulated by CsLOB1 upon Xcc infection. At 36hpi, we used Xcc delivering PthA4 or designer TALE (dTALE) 1 and 2 to activate CsLOB1 and studied the transcriptomes in comparison to leaves infected with pthA4 mutant. dTALE1 and dTALE2 target different effector binding elements in the promoter of CsLOB1, therefore can be used to improve the specificity of CsLOB1 regulon. At 12hpi, we used PthA4-delivering Xcc and pthA4 mutant to study early activated transcriptomes upon CsLOB1 activation. RNA-seq was done using Illumina NovaSeq 6000 to produce 30 million pair-end clusters per sample.