CGZ1589 100X sequencing
To introgress the XZ1516-derived TA system into the N2 strain, we crossed N2 males with XZ1516 hermaphrodites and picked N2/XZ1516 hermaphrodites and cross them with N2 males again. We then repeated the cross for seven rounds and obtained the near isogenetic line named CGZ1589. To make sure CGZ1589 carries the TA system, we cross it with N2 and found rod-like arrested animals among the progeny of N2/CZ1589 hybrids. We then extracted genomic DNA from CGZ1589 using the Puregene tissue core Kit (Qiagen, cat. 1126829). 10 microgram of the DNA was used for library construction and sequencing by BGI (Beijing Genome Institute, China) on the DNBSEQ PE150 platform. The sequencing depth is ~100X.