Effect of the marine natural product-derived compound ZJ-101 on gene expression in MDA-MB-231 3D Spheroids
Four logs of ZJ-101 were tested on 3D spheroid cultures of MDA-MB-231 cells to identify relevant mechanistic pathways through differentially expressed gene analysis Overall design: Triplicate 96-well plates of ultra-low attachment cultured MDA-MB-231 spheroids were treated for 24 hours with four doses (0.5, 5.0, 50.0, 500.0 nM) of ZJ-101 and processed for RNA-Seq DEG analysis