Single cell RNA sequencing immune cells (macrophages, CD4 T and B cells) from male and female mice, at 0,1,2,3 hours after IFNa injection.
Single cell RNA sequencing dataset collected in two independent experiments, including ~24,061 cells (reffered as Dataset A in the paper), and ~13,995 cells (reffered as Dataset B in the paper). Datasets include male and female mice, in 4 consecutive time points - 0h (no IFN, PBS), and 1,2,3 h after IFNa administration. Single cell RNA-sequencing was performed on the 10X platform. Overall design: Data was collected in two independent experiments (Datasets A and B) for a total of 8 male and 7 female mice. In each experiment, C57BL/6J five-week-old male and female mice injected i.v. with IFNa or 100 µL PBS. After 1 h, two mice of each sex (one from the IFN group and one from the PBS group) were sacrificed. After 2 and 3 h, one mouse of each sex in the IFN group was sacrificed. *Mice in Dataset A were littermates; Dataset B did not include a female mouse at 2 h. Hashtag oligos (HTOs) were used to distinguish between mice of different sexes, injection types and time points. From each mouse, CD4 T and B cells from the spleen and macrophages from the peritoneal cavity were double sorted before sequencing. Single cell RNA-sequencing was performed on the 10X platform.