RNA-Seq analysis of SUM159 DAPK3 knockout (KO) cells and control cells
We conducted RNA sequencing of SUM159 DAPK3 KO and control cells followed by Gene Set Enrichment Analysis (GSEA) to identify altered gene expression pathways by DAPK3 knockout. Epithelial-mesenchymal transition (EMT) pathway was the top significantly down-regulated pathway in SUM159 DAPK3 KO cells as compared to control cells. Overall design: mRNA profiles of SUM159 cell lines to identify altered gene expression upon DAPK3 knockout