RNA sequencing to determine gene expression changes in SQLE knockdown osteosarcoma cells
In our study, we found that SQLE was critical to osteosarcoma progression. To identify the mechanism of SQLE in osteosarcoma, we conducted the RNA-seq analysis of SQLE knockdown in osteosarcoma cell line U2OS cells. Overall design: Compare the transcritome profile of U2OS stably expressing SQLE-shRNA or scrambled-shRNA using RNA-seq.