An integrated network pharmacology and RNA-seq approach for exploring the vascular protection of neferine on attenuating Ang II-induced blood pressure elevation
The goals of this study is to compare the differently expressed genes in abdominal aorta tissues of Control and Ang II with or without neferine treatment. The  Ang II  (n=12) were randomly divided into 2 groups: Ang II , and  Ang II +  neferine (1 mg/kg/day) groups (n=6 for each group). Control groups (n=5) were set as control. Mouse in Control and Ang II groups were  intragastrically with double distilled water (dd H2O); while mice in  Ang II +  neferine (1 mg/kg/day) group were intragastrically with 1 mg/kg/day of  neferine for 6 weeks.  Then the abdominal aorta tissues were used to identify differentially expressed genes among different groups. Overall design: RNA sequencing was performed with abdominal aorta of mice in 3 groups:Control(n=5), Ang II(n=6 )  ,  Ang II +  neferine(1 mg/kg/day,n=6) treated to identify the differently expressed genes and related pathway.