Effect of Sirt6 depletion on gene expression of primary human chondrocytes
To define the effect of SIRT6-mediated transcriptional regulation, RNA-sequencing was conducted on primary human chondrocytes depleted of SIRT6 and compared to cells nucleofected with a scrambled siRNA control (72 hours). Overall design: Comparative gene expression profiling analysis of RNA-seq data for chondrocytes nucleofected with siRNA to Sirt6 and controls