Time-Resolved Multi-Omics Analysis of Heat Stress Response in Tomato Plants
Global agriculture and food security are increasingly threatened by heat stress due to climate change and more frequent heatwaves. Tomatoes are susceptible to heat stress in both field and greenhouse settings and further serve as an important model crop for vegetables, so they are a valuable species for heat resilience breeding. To help untangle the complexity of the multi-layered tomato heat stress response and identify target genes, we provide a comprehensive, time-resolved multi-omics dataset of heat stress in tomato: Tomato plants in their young vegetative stage were exposed to heat stress (37?C/28?C day/night) for 6 days and both macroscopic and molecular phenotypes extensively assayed before, during, and after the heat stress phase (i.e. in recovery), with parallel control plants for each time point. This repository contains the total mRNA assays, extracted using the RNeasy Plant Mini Kit (QIAGEN) and sequenced on an Illumina NovaSeq6000.  You can use the PlantID at the end of each sample name (e.g. 2218DP100 or 2226DP172) to connect the sample with the other -omics data which we will publish and link here over time (Phenotyping & FTIR measurement data are available at https://doi.org/10.5447/ipk/2025/3 ). Each plant was phenotyped non-invasively over time but sampled only once and all molecular measurements stem from the same sample.