Effect of PCGF6 loss of function in intestinal adult epithelial cells [ChIP-seq]
We generated RNAseq expression analyses of PCGF6 WT and KO intestinal crypts in two independent replicates using two distinct transgenic models (AhCre and Villin Cre-ERT2) We then performed differential expression analyses of PCGF6 KO vs. WT independently in the AhCre and Villin transgenic models Overall design: Intestinal crypts of 8-12 weeks old sex match (male) mice were collected 7 days post Tamoxifen (Villin) or BNF (AhCre) administration for total RNA isolation followed by SMART-seq NGS library preparation