Copy-number alteration and methylation analysis of intra-cranial masses
The data used in the generation of the iSCORED pipeline for the analysis of brain tumor copy-number alterations and methylation profiling. This data is from intra-cranial masses, including metastatic lesions. Fresh frozen samples had genomic DNA extracted with the Qiagen Uneasy kit. Samples were processed with a the iSCORED assay, which involves a step of restriction digestion and ligation to create chimeric concatemers, which were then processed for Nanopore sequencing using the ligation kit. Please refer to our associated paper for further information on processing and analysis.