eyestalk single-cell RNA sequencing 1
Nitrite stress is a major environmental challenge in Pacific white shrimp (Litopenaeus vannamei) aquaculture, causing immune damage and mass mortality. The eyestalk serves as a key neuroendocrine regulatory center in crustaceans, yet its molecular response to nitrite stress remains unclear. This study aims to elucidate the cellular and molecular mechanisms underlying eyestalk adaptation to nitrite stress using single-cell RNA sequencing (scRNA-seq). By constructing a single-cell transcriptomic atlas of the eyestalk under nitrite exposure, we identified distinct cell subpopulations and their specific stress responses, revealing alterations in energy metabolism, oxidative stress, and neuroendocrine pathways.