Small RNA sequencing for tRF in HepG2 control and SRSF6 knockdown cell
We performed small RNA sequencing in HepG2 cells after SRSF6 knockdown.Total RNA was used for library construction. Briefly, 3' and 5' adapters were ligated to the respective ends of small RNAs. First strand cDNA was then synthesized using reverse transcription primers. The double-stranded cDNA library was generated through PCR amplification. Following purification and size selection, libraries with insert fragments ranging between 18 to 40 bp were subjected to sequencing. Raw sequencing data were processed using fastp to remove adapter contaminated reads, low-quality reads, and reads containing ployN. For tRF analysis, the MINTmap software package was employed to identify and quantify normalized tRF abundances in the small RNA-seq data.