Novel Epigenetic Mechanisms of Endogenous CSE/H2S in Smooth Muscle Cells for Abdominal Aortic Aneurysm [ChIP-Seq]
In this study, ChIP - seq was employed to investigate the impact of CSE on epigenetic modifications in vascular smooth muscle cells (VSMCs) and to uncover its influence on genome - wide DNA binding. We conducted ChIP - seq experiments on H3K9me3, H3K9ac, and H3K4me3 using primary smooth muscle cells from CSE knockout mice and wild - type (WT) mice after 24 - hour stimulation with Ang II. Our finding demonstrated that endogenous CSE/H2S in smooth muscle regulates the expression of extracellular matrix through epigenetic modifications and participates in the occurrence and development of abdominal aortic aneurysm (AAA). Overall design: Primary aortic smooth muscle cells isolated from C57BL/6N mice and global CSE - knockout mice were stimulated with angiotensin II for 24 hours. Subsequently, ChIP - Seq of H3K9me3,H3k9ac and H3k4me3 in primary vascular smooth muscle cells from CSE whole - body knockout mice and C57BL/6N mice.