Identification of Human Pluripotent Stem Cell Derived Astrocytic Progenitors that Correlate with Glioblastoma Subtypes
To investigate the heterogeneity of astrocyte progenitor subtypes present during human astrocyte differentiation that share molecular similarities with tumor initiating stem cells in GBM, we FACS isolated 4 populations of astrocytic progenitor subtypes as identified by the expression of cell surface markers CD51, CD63, and CD71 from three independent iPSC-derived neural progenitor cell lines. Overall design: Comparative gene expression profiling analysis of RNA-seq data for 4 different astrocyte progenitor subtypes identified by the combinatorial expression of CD51, CD63, and CD71.