Mice engrafted orthatopically with glioma and treated with CAR-T cells
C57BL/6J mus musculus mice were engrafted orthatopically with CT-2A glioma, then 6 days days later treated with an intracranial injection of CAR-T cells or negative controls. Two days later, mice were euthanized and brains were collected, and the tumor-bearing hemisphere was dissociated and analyzed by single-cell RNA sequencing.