Single-cell transcriptional profiling of cancer cells in hairy cell leukemia (HCL) patients
This single-cell RNA sequencing (scRNA-seq) dataset profiles human HCL cells from two patients with Hairy Cell Leukemia (HCL) and varient subtype of HCL (HCLv), both expressing unmutated IGHV4-34. scRNA-seq was performed on isolated CD19? and CD3?cells from peripheral blood mononuclear cells (PBMC) (n=2). The dataset enables the characterization of expressed mutation and transcriptional profiles of human HCL cells and supports investigations into cellular lineage and pathways associated with pathologic activation leading to HCL and variant form of HCL (HCLv). Overall design: This study (protocol# 10C0066) was approved by the National Institutes of Health Institutional Review Board (IRB). Fresh peripheral blood were collected from 2 patients diagnosed with HCL undergoing standard-of-care therapeutic treatment. All patients signed IRB-approved informed consent permitting the use of their blood for research. PBMCs were purified from peripheral blood, and cells positive for either CD19 or CD3 were isolated for full-length single-cell sequencing. The expressed mutations and transcriptional profiles were analyzed for identifying the lienage and developmental state of the single cells.