sci-iPS-2sec dataset on hPSC derived Cardiomyocytes at day 23
Proof of principle and testing experiments on sci-RNAseq iPS2-seq have been performed on two batches of hPSC-derived cardiomyocytes at day 21 and 25, respectively. Cells have been treated with Tetracycline throughout differentiation. For this experiment it was used a 2-level of indexing sci-RNAseq protocol (Cao J. et. al, Science 2017). During RT and PCR indexing steps, additional primers have been used to enrich for UCI and shRNA barcodes for the iPS-2 seq pipeline. Sequencing has been performed on a NextSeq 500 with a High Output kit 75 cycles. Read1, 18 cycles; Index1, 10 cycles; Index2, 10 cycles, Read2, 52 cycles.