Barcode lineage tracing of E9.5 and E10.5 whole embryos, barcode-transduced at E7.5 with exocoelomic cavity (ExC) injections
LV-EF1A-H2B-tdTomato-30N barcode virus was injected into exocoelomic cavity (ExC) at E7.5 using ultrasound guided in-utero nano-injections.   E9.5 and E10.5 whole embryos were collected and dissociated. Cells were sorted for tdTomato, and single cell RNA sequenced using 10X Genomics  sequencing.