Gene expression profile at single cell level of non-parenchymal cells from Fibrosis and Tumor (FAT) Metabolic Dysfunction-Associated Steatohepatitis (MASH) model vs healthy liver at 12- and 24-week timepoints.
MASH is a subtype of metabolic dysfunction-associated steatotic liver disease, characterized by inflammation and fibrosis. Non-parenchymal cells in this dataset include mostly immune cells, as well as some other cell types like endothelial cells, cholangiocytes, stellate cells, etc. Overall design: Single cell suspensions from MASH vs healthy murine livers were made using enzymatic digestion with Dnase I and Collagenase IV, as well as mechanical digestion, followed by filtering. Non-parenchymal cells were enriched by using percoll gradients (70% and 25%). Most hepatocytes are removed with this protocol, and red blood cells are lysed. Samples were submitted for scRNAseq, and were analyzed for uPAR expression in this study.