Retinoic acid-induced Transcriptional Signature in Mouse Fibroblast cells
During intestinal wound healing process, wound-associated epithelial (WAE) cells are differentiated from neighboring crypts and migrate to cover the wound bed. We found that WAE cells can produce retinoic acid via aldh1a3 and secrete it into wound bed. WAE cells and fibroblasts are highly colocalized in wound bed, suggesting fibroblasts can potenlially receive RA signal from WAE cells. To identify genes that can be induced by retinoic acid in mouse fibroblast, we performed bulk RNA-seq using NIH3T3 cells. Overall design: To define an inducible RA transcriptional signature on mouse fibroblast, NIH3T3 cells were treated with DMSO or 1uM ATRA for 0h, 8h, 16h.