Raw sequence reads from multiplex PCR amplicon sequencing of Ixodes scapularis nymphs
This dataset consists of 175 host seeking Ixodes scapularis nymphs sequenced with a multiplex PCR amplicon sequencing assay (MPAS). All tick specimens were morphologically identified. This dataset was used to validate a custom bioinformatics pipeline used to analyze MPAS sequences. The assay contained primers targeting the four genera associated with Ixodes transmitted human pathogens: Borrelia spp., Babesia spp., Anaplasma spp., and Ehrlichia spp.