Transcriptomic Profiling of Mouse Glioma GL261 Cells with IDH Mutation and ATRX Loss/Intact (PRJCA046466)
ATRX (alpha-thalassemia/mental retardation syndrome X-linked) is a critical chromatin remodeling factor widely implicated in glioma pathobiology, especially in IDH-mutant low-grade gliomas where ATRX loss frequently occurs. ATRX deficiency synergizes with mutant IDH1 to promote genome instability, modify gene expression networks, and alter tumor phenotypes. Importantly, ATRX loss has been associated with regulation of cellular senescence, the senescence-associated secretory phenotype (SASP), and immune cell recruitment within the glioma microenvironment. This project encompasses both bulk and single-cell RNA-sequencing datasets of these genetically defined GL261 cell models, aiming to elucidate RNA alterations and cellular heterogeneity associated with IDH mutation and ATRX deficiency. The datasets will provide a valuable resource for investigating the interplay between chromatin remodeling, genome stability, cellular senescence, and immune modulation in glioma.