RNA sequencing of bronchoalveolar lavage fluid (BALF) from BPD model rats treated with cholic acid (CA) or control.
To investigate the potential upstream regulators mediating CA's effects, bronchoalveolar lavage fluid (BALF) were isolated from BPD model pups with or without CA treatment. BALF samples were centrifuged to pellet cells. Total RNA was then extracted from the cell pellet using TRIzol reagent for RNA sequencing.