Transcriptomic analysis of StIAA3 and StIAA22 overexpression and interference lines under mannitol-induced osmotic stress in leaves and roots
This project aims to investigate the transcriptomic responses of potato (or your specific species) lines with altered expression of two auxin/indole-3-acetic acid (Aux/IAA) family genes, StIAA3 and StIAA22, under osmotic stress. The study includes overexpression (OE) and interference (RNAi) lines for each gene, alongside the control line (DSR). Plant samples were subjected to either normal conditions (0 mM mannitol) or osmotic stress conditions (75 mM mannitol). Tissues from both leaves and roots were collected for transcriptome sequencing. The generated RNA-seq data will provide insights into the regulatory roles of StIAA3 and StIAA22 in osmotic stress adaptation and organ-specific responses.