Open Chomatin Analysis of human pluripotent stem cell cell-derived FLK1+ vascular mesoderm cells in AATS versus AATSI basal medium
Purpose: Identify accessible chromatin profile between AATS and AATSI condition hPSC-derived FLK1+ mesoderm progenitor cells Methods:ATAC-seq Results: insulin addition (AATSI) led to partial open chromatin status of non-vascular mesderm lineage Conclusions: insulin hindered FLK1+ cells' vascular mesoderm differentiation potential Overall design: Perform ATAC-seq of hPSC-derived FLK1+ vascular mesoderm cells in AATS and AATSI basal medium