Transcriptomic analysis of maize endosperm in miR169o and NF-YA13 overexpression lines at 15 DAP
This project aims to investigate the regulatory roles of the miR169o-NF-YA13 module during maize grain development. We performed comparative transcriptomic profiling (RNA-Seq) using the endosperm of maize kernels at 15 days after pollination (DAP). The experimental groups include miR169o overexpression (OE) lines, NF-YA13 overexpression (OE) lines, and the B104 wild-type background. The objective is to identify downstream target genes and molecular networks controlled by miR169o and NF-YA13 in the developing endosperm, providing insights into the genetic control of maize kernel maturation and nutrient accumulation.