Dose-dependent changes in gonadal white adipose tissue in TCDD-elicited steatohepatitis
Bulk RNA-sequencing of 27 C57BL/6 gonadal white adipose tissue samples where ovariectomized females were gavaged 4 days for 92 days with 0.01 to 30 µg/kg TCDD or sesame oil vehicle (n = 3) per dose. Overall design: Following 4 days acclimation (PND 28), animals (n = 3) were orally gavaged with 0.1 ml sesame oil or 0.01, 0.03, 0.1, 0.3, 1, 3, 10, or 30 microg per kg TCDD (Dow Chemical Company, Midland, MI) every fourth day for 92 days for a total of 23 exposures. Prior to termination animals were fasted for 6 h. Blood was collected by submandibular vein puncture prior to cervical dislocation, centrifuged, and stored at -80 degrees C. Liver and gonadal white adipose tissue (gWAT) were excised, weighed, and frozen at -80 degrees C.