Multi-omics profiling reveals gut microbiota metabolite signatures associated with highfat diet induced obesity-prone and obesity-resistant phenotypes in dogs
Dogs vary widely in their propensity to gain weight when challenged with a high-fat diet (HFD), resulting in obesity-prone (OP) and obesity-resistant (OR) phenotypes; however, the gut microbiota-metabolite mechanisms contributing to this phenotypic divergence remain unclear. Forty-four male Beagle dogs were fed either a control diet (CON, n = 11) or an HFD (n = 33). Within the HFD cohort, dogs were ranked by body weight gain and the upper and lower tertiles were defined as OP (n = 11) and OR (n = 11), serum and fecal samples were collected. The fecal The fecal microbiota was characterized using 16S rRNA gene amplicon sequencing, and untargeted LC-MS metabolomics was used to profile fecal metabolites.