Temperature-sensitive gene identification in gynogenetic gibel carp with temperature-dependent sex determination
we identified the temperature-sensitive period for gibel carp, which is from 11 to 25 days post hatching. During this period, temperature shifts were performed from a high to a normal temperature and from a normal to a high temperature, respectively, and gonadal transcriptome sequencing was conducted after these two temperature shifts. Through the analyses of transcriptome comparison, relative quantitative real-time PCR, and gonad ex vivo culture, a total of 27 gonadal temperature-sensitive genes were identified. The mutation of camsap3l (calmodulin regulated spectrin associated protein family member 3 like), one of the gonadal temperature-sensitive genes, partially prevented high temperature-induced masculinization, indicating that camsap3l is involved in the process of temperature-dependent sex determination or differentiation. These findings build a foundation for further elucidation of the TSD mechanisms and provide new strategies for environmental sex control during genetic breeding in gibel carp.