DENV-2 3'UTR dumbbell structure is a critical factor for viral infection and dissemination in Aedes mosquito (PRJCA039636)
Total RNA was extracted from the midgut of mosquitoes at various time points using the Trizol method and subsequently detected by qRT-PCR. Positive midgut samples were selected, with three pools in each group. Ten positive midgut samples from each pool were combined and three independent biological replicates were prepared for each pool.