Fire-adapted seed traits in Cerrado species
NE/T004851/1 NERC-FAPESP Seedcorn Fund Collaboration Project "Fire-adapted seed traits in Cerrado species" between RHUL (UK) and UNESP (Brazil).  Fire is a global phenomenon which together with climate shapes the vegetation of natural and agricultural land. Our interaction with fire is characterised by both positive and negative aspects for mankind. Humans have long used fire including for landscape and weed management, and as tool to improve crop growth on arable land. Controlled fire is necessary to preserve the health and stimulate rejuvenation of wildland ecosystems such as the Brazilian Cerrado, the Mediterranean, as well as UK peatland and moorland. In these fire-prone regions plant regeneration is achieved to a large extent from soil-stored plant seeds. Depending on the species, environment, season and seed properties, the germination of the soil-stored seeds may be stimulated by compounds derived from the smoke or by the fire-generated heat-shock. The aim of the project is to comparatively investigate seeds from species adapted to fire-prone regions to identify novel mechanisms underpinning fire-generated heat-shock and smoke as germination cues. The derived mechanisms will be tested as tools for weed management and crop seed enhancement. Treatment with smoke and various smoke-derived compounds can stimulate the germination of certain weed seeds. This can be used as a weed management tool to deprive the soil from weeds prior to crop seed sowing. We however do not know why this does not work with all weed species, at all ambient conditions (temperature, seasons), and what seed structures and seed coat properties determine the effectiveness of the treatment. Smoke, various smoke-derived compounds, as well as heat-shock treatment can also improve the seed quality and performance of seedling establishment of certain vegetable crops. Again, we do not know what seed structures, seed coat properties and genes are responsible for these effects and why it only works with certain crop species.   To advance our knowledge in this topic the leading seed science lab of Royal Holloway University of London (RHUK, United Kingdom) will collaborate with experts for fire vegetation management and Brazilian Cerrado species properties of Sao Paulo State University (UNESP, Brazil). The FAPESP-NERC programme is especially suited to support this collaboration based on the agreement of the two funding agencies. In the project we will investigate seeds of different fire-adapted species to identify novel mechanisms controlling how fire-derived smoke and heat-shock affect their germination, storability, and seedling establishment. This work will be conducted using methods from different science and engineering fields (including molecular biology, microscopy/imaging, biomechanical engineering, physiology) through interdisciplinary collaboration in a comparative approach with many fire-adapted species. This approach will for example identify certain seed coat properties or certain genes associated with the adaptation to fire-derived cues. Seeds of weed and crop species with similar properties/genes will then be used to test if the identified novel mechanism has potential for weed control or improving crop seed quality. The consortium has solid fire vegetation management and agri-technological expertise in these applications to provide solutions for this global challenge in climate change, healthy environment and food security.  RNAseq and de novo transcriptome assembly will be used to identify candidate smoke-cue-responsive genes in dry and imbibed seeds of a panel of Cerrado species.