The Effects of Hypoxia on Mesenchymal Stem Cells and the Interventional Role of the YiQi HuoXue formula
Objective: To investigate the effect of hypoxia on the migration of mesenchymal stem cells (MSCs) and the role and mechanism of the Yiqi Huoxue formula in regulating the migration of hypoxic MSCs.Methods: After performing a scratch wound assay, cells were subjected to hypoxia in a hypoxic chamber. The experimental groups were as follows: N0 group (normal culture for 24h without migration), N1 group (migration state under normal culture for 24h), M1 group (hypoxic migration for 24h), M2 group (hypoxic migration for 6h), YH1 group (treated with Yiqi Huoxue formula for 24h).Total RNA was extracted using Trizol, and transcriptome sequencing was performed.