RNA-Seq of importin alpha2 silenced Nicotiana benthamiana
The aim of this project is to examine the gene expressions of Nicotiana benthamiana by importin alpha2 silencing and Bamboo mosaic virus (BaMV) infection. The importin alpha2 of N. benthamiana was silenced by virus-induced-gene-silencing (VIGS) technology. The silenced leaves were infected with BaMV and sampled at 5 days after virus infection. The total RNAs were extracted from these leaves.