Dual roles of fibroblast-epithelial crosstalk in acute and chronic lung injury
Dysfunctional interactions between fibroblasts and epithelial cells contribute to the progression of chronic lung diseases, including idiopathic pulmonary fibrosis. Our findings showed that chronic epithelial damage initiates a bidirectional fibrotic cascade between the epithelium and lung fibroblasts, leading to further epithelial damage and the release of pro-fibrotic mediators. Conversely, our transcriptomic and proteomic analyses highlighted that, in the context of acute epithelial injury, a protective signaling environment merges that mitigates further damage. We observed that epithelial cells shift their metabolism towards increased aerobic fatty acid metabolism, promoting healing and repair. By delineating secreted regulators responsible for inducing these positive responses, we identified pentraxin 3 (PTX3) as a top hit harboring antifibrotic characteristics. Overall design: To explore the interplay between epithelial cells and fibroblasts in the human lung following injury, we developed a coculture system utilizing mature, differentiated primary small airway epithelial cells (SAECs) in an air-liquid interface and primary human lung fibroblasts (LFs). We investigated in two distinct scenarios that mimic chronic and acute injuries. In the chronic injury model, SAECs pre-treated with TGF-ß1 for 14 days (tSAECs) were employed, which were subsequently co-cultured with LFs or as monocultures for an additional 3 days in the presence of the stimulus. Conversely, no prior injury was induced in the acute model (nSAECs), but cells were cultivated, before the initiation of the coculture and TGF-ß1 treatment.