Gallus gallus Raw sequence reads
The purpose of this study was to investigate the effects of CHC on growth performance, immunity, intestinal flora and liver metabolism of Mountain Plum Blossom Chicken (MPBC). A total of 400 130-day-old male MPBCs were randomly divided into 4 groups of 10 replicates of 10 in each group. Chickens were fed supplemental basal feed with 0 (CG), 0.25 (EG1), 0.5 (EG2), and 1.0 (EG3)% CHC between days 130 and 172. The results showed that Dietary supplementation with 0.25% CHC (EG1) most effectively enhanced growth performance and immune function in MPBC, as evidenced by significantly increased ADG and IgM levels, along with the clearest separation from the control group in PCA analysis of serum and growth parameters. The results of serum biochemical indexes showed that compared with CG, the IgM level in the EG1 group was significantly increased (P < 0.05), and the IgG concentration in the EG2 group was also significantly increased (P < 0.05). In the EG3 group, the levels of IL-10 (P < 0.05; Figure I) and IgM (P < 0.01; Figure M) increased significantly, and the increase in IgM reached a very significant level (P < 0.05). Feeding chickens containing 0.4% and 0.6% TCP between 43 and 105 days resulted in a decrease in serum IL-2 concentration and an increase in IL-4 content (P 0.05<). Chickens fed 0.4%, 0.6% and 0.8% TCP had significantly higher jejunal villus height (P <4, 0.05), and TCP supplementation also significantly increased the relative abundance of Bacteroidota compared with the control group (P < 0.05).