Neutrophil Degranulation is Increased at Seven Days after Human Intracerebral Hemorrhage,   but not at 72 Hours, and Correlates with Decreased miR-3613 and miR-3690
Intracerebral hemorrhage (ICH) occurs when there s spontaneous, non-traumatic bleeding into the brain and accounts for 50% of stroke-related mortality. We report mRNA-seq and miRNA-seq in the peripheral blood of ICH patients with three serial samples during the first week after the stroke. Twenty-seven ICH patients were enrolled via 24/7 screening and peripheral blood sampled at <24 hours (baseline), 72 hours (+/-12 hours), and 7 days (+/- 2days) from last known normal. mRNA-seq and miRNA-seq were assessed for differential expression (DE) between the time points and pathways identified via enrichment analysis as well as principal component analysis (PCA) were performed. Our analysis found that neutrophil degranulation was the prominent enriched pathway at 7 days after ICH. miR-3613 and miR-3690 are potential regulators of neutrophil degranulation after ICH. Overall design: A total of 27 ICH patients were enrolled and 3 serial blood samples were collected for <24 hours, 72 hours and 7 days after stroke. After mRNA and miRNA sequencing, differentially expressed (DE) miRNA and mRNA will be tested between 3 time points: 72 hours vs. baseline, 7 days vs. 72 hours, and 7 days vs. baseline will be tested using pathway analysis.