16SrRNA reveal how green tea polyphenols against the Eimeria tenella infection in Wuliangshan black-bone chickens
Wuliangshan black-bone chickens were randomly divided into 5 groups with 15 chickens in each group. The chickens in 1-3 groups were treated with green tea polyphenols according to the body weight as follows: orally treated the total dose was 50mg water solution per weight kilogram (group 1), orally treated the total dose was 200mg water solution per weight kilogram (group 2), orally treated the total dose was 350mg water solution per weight kilogram (group 3). By contrast, group 4 was not treated with any tea polyphenols, whereas group 5 was the blank control group. The chickens were treated with green tea polyphenols from the age of 8 days to 28 days. The chickens in group1 to group 4 were orally infected with 50,000 sporulated E. tenella oocysts at 21 days old. At 28 days old, according to the ACI indexes, we choose the caecal contents of group 2 (green tea polyphenols treated and infected with Eimeria tenella), group 4 (infected with Eimeria tenella) and group 5 (not treated) for 16SrRNA sequencing.