RNA Sequencing of miR-10b +/+ and miR-10b -/- Mouse Embryonic Stem Cells During Adipocyte Differentiation at Days 0, 12, and 27
The experiment was designed to investigate the sequence of events regulating preadipocyte commitment and terminal differentiation into adipocytes, providing insight into brown fat physiology and metabolic dysfunction. miR-10b was found to be upregulated in brown adipocytes, and CRISPR/Cas9 was used to generate its functional knockout (KO) in E14 mouse embryonic stem cells (ES). Transcriptomic analysis was performed at days 0, 12, and 27 to identify pathways affected by miR-10b depletion.