Effect of Sox7 endothelial conditional knock out on blood vessel differentiation in vivo in a mouse model [Bulk RNA-seq]
To study the effects of the loss of Sox7 function on gene expression of the endothelium in vivo during development. We generated a endothelial specific ko using a Sox7fl/fl Cdh5 creERT2 inducible system. Overall design: Gene expression profiling analysis using RNA-seq (polyA) of CD31-positive endothelial cells sorted from Sox7 inducible endothelial conditional knock out or sibling control embryos at 10.5dpc Comparative gene expression profiling analysis of RNA-seq data for wild type control and Sox7 deleted endothelial cells (homozygous deletion). There are 3 wt ctrl embryos and 3 wt homozygous embryos that have been processed in the same batch to allow for pairwise comparison.