RNA-seq of human visceral and subcutaneous adipose tissue with donors grouped by COVID-19 antibodies
Previous findings suggest that adipocytes may play a role in adverse COVID-19 outcomes in people with obesity. To investigate the impact of COVID-19 vaccination and infection on adipose tissue, we generated 27 paired biopsies of visceral and subcutaneous adipose tissue from donors of the Leipzig Obesity BioBank (LOBB) that have been categorized into three groups based on their COVID-19 antibodies to spike (indicating vaccination) and/or nucleocapsid proteins.RNA extraction for Transcriptomic analysis from tissues was performed using the RNeasy Lipid Tissue Mini Kit (QIAGEN GmbH, Hilden, Germany) according to manufacturer's instructions. RNA samples were quantified and their integrity checked before performing rRNA and globin depletion, followed by library preparation and sequencing using Illumina NovaSeq 6000 by the Leipzig laboratory of GENEWIZ Germany GmbH. RNA library preparation was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturer's instructions (NEB, Ipswich, MA, USA). The samples were sequenced using a 2x150 Pair-End (PE) configuration.