ATAC-seq for PHF8-knockout CT26 cell and parental cell.
CT26 cells with or without PHF8 knockout were processed using the High-Sensitivity Open Chromatin Profile Kit according to the manufacturer's instructions.DNA was extracted using Tagment DNA Extract Beads, purified with NovoNGS DNA Clean Beads, and amplified by PCR. Libraries that passed quality control were sequenced on an Illumina NovaSeq X Plus platform. Overall design: We compared the chromatin open profile of control and PHF8 knockout CT26 cells. Two biological replicates are set for each group.