Single-cell RNA sequencing of hippocampal progenitor cells and their progeny under TNF treatment
This study used single-cell RNA sequencing to characterize transcriptional responses of a hippocampal progenitor cell line (HPC0A07/03A) and its differentiated progeny to tumor necrosis factor (TNF) exposure. Cells were profiled following short-term TNF treatment under proliferative conditions, as well as during differentiation under control or chronic TNF exposure. Sample multiplexing was performed using TotalSeq-B hashtag oligonucleotides, enabling pooled library preparation and sequencing. These data provide a resource for investigating how inflammatory signaling influences hippocampal progenitor cell states and differentiation at single-cell resolution.