Bifidobacterium adolescentis CCFM1285 combined with yeast yeast-glucan alleviates gut microbiota and metabolic disturbances in mice with antibiotic-associated diarrhea
Antibiotic-associated diarrhea (AAD) is a self-limiting condition that can occur during antibiotic therapy. Our previous studies have found that a combination of Bacteroides uniformis and Bifidobacterium adolescentis can effectively alleviate AAD. However, the use of B. uniformis is still strictly limited. Therefore, this study attempted to use yeast yeast-glucan to enrich the abundance of B. uniformis in the intestine and supplement Bifidobacterium adolescentis to exert a synergistic effect. The lincomycin hydrochloride-induced AAD model was administered yeast yeast-glucan or a mixture of B. adolescentis CCFM1285 by gavage for one week. Subsequently, changes in colonic histopathological structure, inflammatory factors, intestinal epithelial permeability and integrity, metabolites, and gut microbiota diversity were assessed. We found that yeast yeast-glucan, alone or in combination with B. adolescentis CCFM1285, can help attenuate systemic inflammation, increase the rate of tissue structural recovery, regulate metabolism, and restore gut microbiota. Specifically, the combination of yeast yeast-glucan and B. adolescentis CCFM1285 was more effective in decreasing interleukin-6 levels, improving pathological changes in the colon, and upregulating occludin expression. Therefore, our study showed that the combination of yeast yeast-glucan and B. adolescentis CCFM1285 is an efficacious treatment for AAD.