149 Neisseria meningitidis genomes
The DNA of 149 isolates from Suzhou was purified and recovered by a silica gel column (D3146, HiPure Bacterial DNA kit) after incubation. Paired-end libraries with insert sizes of ~300 bp were prepared following Illumina standard genomic DNA library preparation procedure (VAHTS Universal DNA Library Prep kit for Illumina V3) and sequenced on an Illumina NovaSeq 6000 using the S4 reagent kits (v1.5) according to the manufacturer's instructions. The sequencing reads of each isolate were quality trimmed and assembled into contigs using EToKi. In addition, 4,411 assembled genomes were downloaded from the GeneBank database and 67,328 sets of short reads associated with Neisseria in the NCBI SRA were also assembled using the EToKi assemble pipeline?