Analysis of the role of PTB and HuR on neuronal differentiation and stress response
In this work, we conducted shRNA mediated Knockdown of HuR and PTB in order to analyze their impact on neuronal differentiation of SHSY-5Y neuroblastoma cell line as well as on the stress response of these cells. RNA-seq libraries were conducted on poly-A enriched mRNA using NEBNEXT Ultra ii directional RNA library prep kit