Sequencing analysis of gene expression in Arabidopsis under high-temperature stress
The PtHSF5 gene from Pinellia ternata enhances high-temperature tolerance in Arabidopsis. To identify downstream genes regulated by this transcription factor, we conducted transcriptome sequencing analysis of both PtHSF5-transgenic Arabidopsis and wild-type Arabidopsis under 2-hour heat stress. Subsequent differential expression analysis revealed candidate downstream genes regulated by PtHSF5 during thermotolerance enhancement in Arabidopsis.