Daidzin Target mutant sequence reads
Targeted gene editing and gene deletion strains of Phytophthora capsici were generated for genes encoding putative daidzin receptor proteins using CRISPR-Cas9 and homologous recombination strategies. High-quality genomic DNA was isolated from individual mutant and control strains and used for Illumina-based second-generation sequencing. The resulting reads data were used for comparative analyses to confirm targeted gene deletions or edits, to examine edited genomic regions, and to assess potential off-target effects associated with the guide RNAs used during editing.