RNA sequence of the DXR-treated, INK4a-transduced, or CI-induced naked mole-rat fibroblasts
To investigate gene expression in naked mole-rat (NMR) cells after cellular senescence induction, mRNA-seq was performed in doxorubicin (DXR)-treated, INK4a-transduced, or contact inhibition (CI)-induced NMR fibroblasts. For DXR treatment, NMR fibroblasts were treated twice with 100 nM of DXR for 24 hours. DXR-treated NMR fibroblasts were cultured for 21 days and they showed several features of cellular senescence, including larger and flat morphology, a decrease in the BrdU incorporation, an increase in the SA-beta-Gal activity, and increased INK4a expression. Control fibroblasts were not treated with DXR and were harvested 3 days after seeding. The data is deep sequencing of mRNA from three primary NMR fibroblasts (D43, GH30, and GH33). Gene expression profiles of these cell lines were analyzed. For INK4a transduction, NMR fibroblasts were lentivirally transduced with INK4a. INK4a-transduced NMR fibroblasts were cultured for 12 days and they showed several features of cellular senescence, including larger and flat morphology, decrease in the BrdU incorporation, increase in the SA-beta-Gal activity, hypophosphorylation of Rb protein and phosphorylation of AKT protein. Control fibroblasts were transduced with empty vector and harvested at 6 days to keep the numbers of passage equivalent to those of INK4a-transduced cells. The data is deep sequencing of mRNA from three lines of NMR-fibroblasts (C42, H45-2, and D2). Gene expression profiles of these cell lines were analyzed. For CI induction, NMR fibroblasts were seeded at 6 Ã? 105 cells/10-cm dish and cultured for 28 days. They showed increased SA-beta-Gal activity, decreased BrdU incorporation, and increased INK4a expression. Control fibroblasts were harvested 1 day after seeding. The data is deep sequencing of mRNA from three primary NMR-fibroblasts (L14, H44, and H45). Gene expression profiles of these cell lines were analyzed.