Spatial transcriptomics of smooth muscle cell in human atherosclerosis
Three different coronary artery plaque types (erosion, rupture, and adaptive intimal thickening) were selected from females heterozygous for the deletion BGN. Hybridization of target RNA-specific probes was used to visualize the spatial distribution of mutants. A clonality index was calculated from the percentage of each probe in each region of interest (ROI). Spatial transcriptomics was used to identify differentially expressed transcripts within clonal and non-clonal regions.