RNAseq of Atlantic salmon with CRISPR/Cas9-mediated mutation of delta-5 and multiple delta-6 fatty acyl desaturase genes
The present study used CRISPR/Cas9 technology to generate two knockout strains of salmon by mutating 1) all fads genes simultaneously (delta-5fad, delta-6fad-a, delta-6fad-b and delta-6fad-c), and 2) delta-6fad-b and delta-6fad-c genes. Liver was taken and RNA-seq was used.