This study aims to investigate rumen microbial isolates, particularly anaerobes, derived from Lambs Ovis aries, Lactating goats Capra hircus, and kids Capra hircus. These isolates were obtained from enrichment using modified Hungate medium with pectin as a substrate. Utilizing a whole genome sequencing approach, the research seeks to understand the culturable anaerobic isolates from the rumen fluid and potential of the same by analyzing the biomolecules.