Multiomic Profiling Reveals Metabolic Alterations Mediating Aberrant Platelet Activity and Inflammation in Myeloproliferative Neoplasms [human RNA-Seq]
To investigate the effects of a-KG on monocyte gene expression, magnetic beads-sorted CD14+ human monocytes from MPN patients (PT, n = 3) and healthy individuals (HI, n = 2) were incubated with a-KG (250µM) or DMSO control for 6 hours. Cell pellets were collected for RNA-seq analysis. Overall design: gene expression profiling analysis of human primary monocytes