Metagenome Assembled Genomes resolved using GC-fractionation
The data consist of short-read Illumina metagenomic sequencing of twelve samples from soil, freshwater sediments and marine sediments collected August 2020 with the aim to retrieve metagenomic-assembled genomes for members of the Phylum Myxococcota. This will be achieved by co-assembly of different GC fractions of the metagenome, with different fractions harbouring different fragments of DNA belonging to taxa with different GC content. Fractionation was achieved using cesium chloride gradient ultracentrifugation (as described for separation of DNA fractions labelled with isotopes (DNA-SIP) (Nicol and Prosser, Methods in enzymology, 2011)).