The impact of estrogen receptor bata activation in colonic epithelial cells on downstream signaling pathways
Total RNA was extracted from both estrogen receptor bata agonists DPN treated colonic epithelial cell lines NCM460 cell samples and untreated control samples utilizing Trizol reagent. To accurately determine the concentration and purity of total RNA in each sample, the Nano 300 spectrophotometer was employed. Subsequently, these total RNA samples were submitted to Shanghai Bioprofile Biotechnology for professional processing.