The kidney transcriptome analysis elucidates an understanding of the molecular process affected by bisfenol A.
Bisphenol A (BPA), is a chemical substance used in the production of polycarbonate plastics such as food containers, healthcare equipment, products for children (toys, soothers, milk bottles), or thermal paper. According to US Environmental Protection Agency, BPA belongs to endocrine-disrupting chemicals and exerts a pathogenic effect in several disorders and affects both female and male reproductive systems. Moreover, BPA exposure enhances carcinogenesis and inflammatory or immune responses. BPA metabolism takes place mainly in the liver, and most conjugates generated by BPA with glucuronides and sulfates are excreted into urine or faeces after a few days. Some recent studies have revealed, that BPA contributes to kidney damage. The present study was designed to explore the specific molecular changes triggered in these organs after long-term oral exposure to BPA in mice. The experiment was carried out on 14 3-month-old female mice. The animals were divided into two groups: 6 mice served as controls and were not subjected to any experimental procedures. The other 8 mice served as an experimental group and were treated with BPA in drinking water at a dose of 50 mg/kg b.w. for 3 months Both groups of mice were given the same food, they were also weighed weekly, and the BPA dose was increased on this basis. After 3 months, the animals were sacrificed by decapitation. Immediately after death, the kidneys were removed under sterile conditions, protected, placed in liquid nitrogen and stored at -80°C until analysis was performed. Within both the experimental and control groups total RNA was isolated and high-throughput RNA sequencing was used to analyze the global changes in the transcriptome of the BPA-treated kidneys.