A Human iPSC-derived Microphysiological Blood-Brain Barrier Model for Permeability Screening
Human induced pluripotent stem cells (hiPSC)-derived endothelial cells were cultured in a transwell (2D) or microfluidic system (3D) to create in vitro models of the blood brain barrier (BBB). High-throughput sequencing of the endothelial cells  was performed to investigate transcriptional differences between the culturing conditions related to cellular properties in the BBB, including endothelial cell markers and transporter activity.