Nanopore sequencing of plasmids containing HAdV subtypes
Plasmids containing full-length Fiber, Penton, and Hexon genes from different HAdV subtypes including HAdV11, 14, and 55 were synthesized. Partial gene fragments (amplicons) covering true deletions were also amplified using PCR primers. The samples were mixed and sequenced using R9.4.1 flow cell (n=9).