Transcriptomic Responses of Pantoea, Micrococcus, Pseudomonas, and Herbaspirillum to Ferric Chloride (FeCl3) Treatment Transcriptome
This study presents a comparative transcriptomic analysis of Pantoea, Micrococcus, Pseudomonas, and Herbaspirillum under ferric chloride (FeCl3) treatment. Bacterial cultures were grown with and without FeCl3, and RNA sequencing was performed to evaluate global gene expression changes.FeCl3 exposure resulted in significant differential expression across all four genera. Genes involved in iron acquisition, including siderophore biosynthesis and iron transport systems, were generally upregulated, indicating active regulation of iron homeostasis. In addition, oxidative stress response genes, such as those encoding catalase and superoxide dismutase, showed increased expression, suggesting enhanced reactive oxygen species stress under FeCl3 conditions.Metabolic responses varied among genera. Pseudomonas and Herbaspirillum exhibited changes in central energy metabolism, while Pantoea and Micrococcus showed stronger regulation of transport and cell envelope related functions. Overall, these data provide insight into conserved and strain specific transcriptional responses to iron availability in bacteria.