Dataset of histone H3K4me2 modified genes in the liver of female Sprague-Dawleyrats with chronic antipsychotic drugs of olanzapine or clozapine
Epigenetic histone modificationshave been found to be associated with the development of metabolic disorders.However,the potential contribution of these modifications to metabolic disturbances induced by chronic treatment with second-generation antipsychotic drugs (SGAs) remains unclear. This study presents chromatin immunoprecipitation (ChIP) data on H3K4me methylation in hepatic tissue of rats treated with olanzapine or clozapine for 9 weeks.This dataset provides a comprehensive view of the effects of SGAs on H3K4me methylation in an animal model, offering new insights into the epigenetic mechanisms underlying SGAs-induced metabolic side effects. Overall design: Thirty-six female Sprague-Dawley rats (200g- 220g) were purchased from the Animal Resource Centre (Perth, WA, Australia). After one week of environmental adaption, the rats were randomly assigned into three groups: olanzapine (3 mg/kg, b.i.d, orally, Zyprexa, Eli Lilly, Indianapolis, USA), clozapine (20mg/kg, b.i.d, orally, Clozaril, Novartis, Turkey), and vehicle (equivalent sweet cookie dough pellet without drugs) with 12 animals per group. The antipsychotic drugs were mixed into 300mg sweet cookie powder (30% cornflour, 30.9% sucrose, 15.5% casein, 8.4% minerals, 6.4% fibre, 6.3% gelatine, 1.6% vitamins) and appropriate water. Each animal was treated orallytwice a day (8:00 am and 8:00 pm, respectively) for nine weeks, as described previously (Liu et al., 2017). During the treatment, rats were observed to completely consume drugs pellet. Since the rats avoided taking the cookie pellet with clozapine after 3 days' treatment, clozapine was delivered using a 1 ml syringe via the mouth, followed by an equivalent sweet cookie dough pellet without drugs to ensure the same amount of cookie pellets was consumed. All animals were housed individually in temperature-controlled room (220C) with a 24-hour lighting cycle (lights on 07:00-19:00), allowed to free access water and standard laboratory chow diet (containing 3.9kcal/g, 10% fat, 16% protein and 74% carbohydrate) throughout the experiment. Body weight, food intake and water consumption were recorded every week. Two hours after the final time treatment, all fasting rats were sacrificed by carbon dioxide asphyxiation. Liver tissues were dissected and frozen in liquid nitrogen immediately.