M. leprae transformed with BRET-nanoluciferase expressing plasmid pYUB3052
M. leprae Thai-53 was transformed with an integrative plasmid pYUB3052 expressing BRET-nanoluciferase cassette, GeNL to construct reporter strain of M. leprae. The transformants were grown in nude mice foot pads and harvested after two passages of six months each. The genomic DNA was isolated and subjected to deep sequencing