Genes induced by PDGF-BB in cells lacking Signal Transducer and Activator of Transcription-1 (Stat1)
Comparison of gene expression between Stat1+/- and Stat1-/- dermal fibroblasts or vascular smooth muscle cells after treatment with PDGF-BB for one hour. Overall design: Dermal fibroblasts (DF) and aortic vascular smooth muscle cells (VSMC) were isolated from two Stat1+/- mice (wt) and two Stat1-/- mice (mut). Cells were serum starved in DMEM + 0.1% serum for 24 hours, then treated with 30ng/mL PDGF-BB for 1 hour or left untreated, then harvested for RNA.