Immunotoxicity and mechanism analysis of zebrafish embryos exposure to Benzothiazole and its derivatives
The 6 hpf zebrafish embryos were randomly placed into 24-well plates with 10 embryos/well. The BTH and OBT exposure concentrations were 50 M, 100 M, 200 M. The larvae exposure with 200 M BTH and larvae in control group were collected (100 larvae each group). RNA was extracted by Total RNA Isolation Kit (Yi Fei Xue Biotechnology, Nanjing, China).