Time-resolved population genomic analysis of Vibrio alginolyticus strain Va2225 during 30-day in vitro coevolution with a four-phage cocktail targeting distinct receptors.
This project provides a high-resolution genomic time series capturing the coevolutionary arms race between a pathogenic marine bacterium, Vibrio alginolyticusstrain Va2225, and a therapeutic bacteriophage cocktail. The dataset stems from a 30-day serial-passage evolution experiment, where the bacterial host was challenged with a cocktail of four distinct phages, each utilizing different host receptors for infection. Population genomic sequencing was performed at 10 critical time points throughout the experiment.