16S rRNA gene sequencing in feces from WT and Sox9LoxP/LoxP;Villin-CreERT2 mice using Illumina MiSeq nano (GenoScreen company).
The taxonomic and phylogenetic resolution of microbial community profiling via 16S rRNA gene sequencing was performed in feces from WT (n=8) and Sox9'LoxP/LoxP';Villin-Cre'ERT2' mice (n=8) targeting V3-V4 region in r16 rDNA, by GenoScreen company. Amplicon library preparation was performed using Metabiote process. Library sequencing generated 2x250 bp "paired-end" reads in a single run using Illumina MiSeq nano. Sequences were sorted by samples and assembly of "paired-ends" reads allowed to obtain full length 16S rDNA readings.