RNA-seq of 8-week-old male mouse pancreas: Targeted delivery of IFN-gamma mRNA and MPLA for macrophage reprogramming
To investigate the mechanisms by which antibody-conjugated liposomes loading IFN-gamma mRNA and monophosphoryl lipid A (MPLA) induce anti-tumor immune responses via targeted reprogramming of macrophages, we performed RNA sequencing on pancreas tissues from 8-week-old male mice. A total of 48 samples were collected across various experimental and control groups. This comprehensive transcriptomic profiling aims to elucidate the in vivo impact of targeted IFN-gamma delivery on the pancreatic microenvironment, specifically evaluating macrophage polarization states and downstream immunological alterations.