Isolation, Morphological, Physiological, Biochemical and Molecular Characterization of Actinobacteria Members from White and Red Soil Samples
Members of the genus Streptomyces in the Actinobacteria phylum have led to the development of a number of antibiotics which are their secondary metabolites or modified derivatives. Antibiotic production increases the competitiveness of Streptomyces. The reason for the continuous production of antibiotics is that bacteria have a resistance mechanism. White soil samples and red soil were collected for the research. Genotypic characterization of S.griseorubens, S.griseus, S.griseoincarnatus, S.albogriseolus and Nocardiopsis dassonvillei isolates were obtained from these soil samples.In the preliminary study, it was observed that Streptomyces isolates showed antimicrobial activity. From this study, species belonging to the genus Streptomyces and species belonging to different genera of Actinobacteria will be isolated from these soils. After the phenotypic and genotypic characterization of the new isolates, antimicrobial activity screening will be performed. Since different culture conditions greatly affect the secondary metabolite biosynthesis of the organism, optimum pH and temperature values of all isolates exhibiting antimicrobial activity are predicted to be produced at the highest yield. After optimization, the effect of polymers on bulk growth of Streptomyces isolates will be investigated. Those in the center of this structure age earlier than those on the edge and begin to produce secondary metabolites. When the polymers are added to the medium, the effect of opening this structure and ensuring the simultaneous growth of all cells will be investigated on antimicrobial activity.