The expression profile of relevant mRNAs in the DRG following conditional pre-injury.
Conditioning injury induces transcriptional responses in the DRGsStudies have confirmed that PNS injury upregulates regeneration-related genes in a transcription-dependent manner, thereby improving axonal transport efficiency and the function of neuronal somatosensory branches. However, a similar regenerative phenomenon has not been observed in the CNS. Previous studies on CI have mostly focused on the upregulation of regeneration-related genes induced by pre-injury.We first crushed the sciatic nerve to achieve CI. Subsequently, dorsal root ganglion (DRG) neurons of the L3-L5 level at 7 days post-operation were collected at 7 days post-injury (the number of animals in each group was 4, and 6 DRG tissues were obtained from each animal), followed by mRNA transcriptomic sequencing. To investigate the differences in transcriptome changes following CI of the PNS, we established four experimental groups: the sham group (sham operation group, suturing after spinal laminectomy), the SCI group (spinal cord crush injury group), the Crush group (sciatic nerve crush-only), and the CI group (PNS pre-injury combined with spinal cord crush injury group). After performing mRNA transcriptome sequencing on the DRG tissues, bioinformatics analysis was conducted to screen for genes associated with the observed cellular processes or characteristics. Using a screening criterion of P<0.05 and |logFC|>1, comparative analysis among the groups revealed the following: A total of 1,315 differentially expressed genes (DEGs) were identified between the CI group and the Sham group, with 1,126 upregulated and 189 downregulated.