A novel CRISPR/Cas9 screening platform identifies an IRF1-SOCS1-mediated negative feedback loop that limits CXCL9 expression and anti-tumor immunity (IRF1 ChIP-Seq)
CHIPseq of mouse IRF1 in macrophage and AT-3 breast cancer Overall design: Murine bone marrow derived into macrophages using GM-CSF. Macrophages were stimulated with 1ng/mL IFNg for 3 hours. AT-3 cells were stimulated for 5ng/mL IFNg/TNF for 3 hours. Anti-Irf1 immunoprecipitation was performed.