Analysis of differential miRNA expression in mineralising DPCs treated with pharmacological epigenetic inhibitors.
To establish a miRNA expression profile for DPCs undergoing epigenetically-mediated mineralisation, rodent DPCs were induced to mineralise and treated with a HDAC inhibitor, SAHA, and a DNMT inhibitor, 5-AZA-CdR. RNA was then isolated from DPCs at day 4 of culture and subjected to RNA sequencing. Subsequent bioinformatic analysis identified differentially expressed miRNAs compared with untreated mineralising DPCs. Overall design: Identification of differentially expressed miRNAs in mineralising rodent DPCs treated with epigenetic inhibitors compared with untreated mineralising DPCs and with non-mineralising DPCs.