Effect of Sirt7 depletion on gene expression of primary murine vascular smooth muscle cells isolated from c57bl/6
To investigate the functional role of Sirt7 in VSMCs calcification, we knockdowned Sirt7 expression in VSMCs using siRNAs, and performed RNA-seq to identify the downstream genes regulated by Sirt7 Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cells transefected with siScrambled and 3 different cells transfected with siSirt7.Comparative gene expression profiling analysis of RNA-seq data for control VSMCs and VSMCs with Sirt7 depletion