Chip-seq to identify sE dependent genes in EtOH stressed and unstressed Streptomyces coelicolor A3(2)
Chip-seq experiment used to identify the binding sites of alternative s factor sE in Streptomyces coelicolor. We access the binding sites with and without EtOH stress condition (see methods). To capture the binding of sE, the sE gene was tagged on genome by HA (Human Influenza hemagglutinin derived) epitope (TAC CCA TAC GAC GTC CCA GAC TAC GCT) on its C-terminus, wild type strain without tagged sE served as negative control.