RNA sequencing of RAW264 cells treated with or without RANKL
RAW264 cells were treated with RANKL (100 microG/mL) to induce osteoclastic differentiation. Undifferentiated cells and immature osteoclasts that did not adhere onto the dishes were removed by treating with Pronase E. Then, total RNA was isolated from the adherent osteoclastic cells and subjected to RNA sequencing to identify genes that play an important role in osteoclastic differentiation..