Role of KDM6A in HSPC Emergence and Myelopoiesis (PRJCA030354)
KDM6A, a H3K27 demethylase and key component of the COMPASS complex, is frequently mutated in hematological malignancies. Despite its known epigenetic regulatory functions, KDM6A's roles in embryonic hematopoiesis and tumor suppression in chronic myelomonocytic leukemia (CMML) are not fully elucidated. The zebrafish (Danio rerio, Dr) genome and hematopoietic system closely resemble those in humans, serving as an excellent model to study hematopoiesis, particularly during early embryogenesis. Utilizing zebrafish models with various kdm6a mutants, we conducted RNA sequencing (RNA-seq) to explore the underlying molecular mechanisms of kdm6a during hematopoietic stem and progenitor cell (HSPC) emergence and differentiation.