Study on the effect of Oleo-Gum Resin of Commiphora wightii and its active principles on lipoprotein lipase and lipid droplet motility during adipogenesis
Murine 3T3-L1 preadipocytes were obtained from the National Centre for Cell Science (NCCS) in Pune, India. These preadipocytes were seeded in either 6-well or 12-well plates or T25 flask and cultured in Dulbecco's Modified Eagle's Medium with L-glutamine and 4.5 g/L glucose (MP Biomedicals) containing 10% fetal bovine serum and 100 ug/mL penicillin-streptomycin. at 37C in 5% CO2. The cells were grown until they reached 95% confluence. On day 0, differentiation was induced using 1 uM dexamethasone, 0.5mM 3-isobutyl-1-methylxanthine and 10 ug/mL insulin. After two days, the medium was replaced with DMEM containing 10 ug/mL insulin. This was done every two days until day eight when the medium was changed again. By then the cells had fully matured and differentiated into adipocytes. The differentiated cell treated with ethanolic extract of OGR of Commiphora wightii at 50 ug/mL and 80 ug/mL, identified active ingredient tyramine hydrochloride 20 ug/mL and reference drug orlistat 10 ug/mL for 24hrs.