Effects of hypoxia on the transcriptome of liver exosomal mirna in Trachinotus blochii
Hypoxia experiment: normoxic and hypoxic groups. Three replicate culture buckets were set up for each group. One hundred and twenty-six healthy and active golden pompano of similar size were randomly assigned to these six breeding buckets, and the feeding of these fish was suspended one day before the experiment. Three buckets were randomly selected as the control group without hypoxic stress, maintaining sufficient dissolved oxygen and other conditions, as the normoxic group and recorded as N. The remaining three buckets were used as the hypoxic group and recorded as H. At the beginning of the experiment, aeration of the buckets in the hypoxic group was turned off and sealed with plastic film, and the dissolved oxygen was lowered to 2.0 mg/L within two hours by filling with nitrogen and maintained at this level for 12h. The aeration of the buckets in the hypoxic group was turned off and sealed with plastic wrap, and the dissolved oxygen was reduced to 2.0 mg/L by filling with nitrogen.