EBF1 regulates sensory establishment in the cochlea by positioning the medial boundary of the prosensory domain and restricting proliferation of the sensory progenitor population
In our previous study, we reported that Ebf1 excision throughout the inner ear epithelium and before the onset of cochlear development leads to dramatic sensory expansion in the cochlea by neonatal stages. Ebf1 conditional knockout cochleae possess over twice as many sensory cells as littermate controls and develop ectopic sensory patches in their Kölliker's organs. To better understand the mechanism behind EBF1's role in restricting sensory establishment, we performed multiome sequencing in our current study. EBF1 is a traniption factor best known for its importance in B cell lineage specification, during which it acts as both an activator and a repressor. Our results indicate that EBF1 prevents the Kölliker's organ cells from being recruited to the prosensory domain by promoting expression of Prdm16 and repressing expression of Jag1 and Sox2. We also found that EBF1 may promote cell cycle exit by repressing Ccnjl expression. In summary, medial expansion of the prosensory domain, together with delayed cell cycle exit in the developing cochlear epithelium, underlies the robust increase in sensory cells seen in Ebf1 conditional knockouts. Overall design: To enable investigation of EBF1-dependent changes in cochlear development at the single cell level, we performed multiome sequencing, combining single nucleus RNA sequencing (snRNA-seq) and single nucleus ATAC sequencing (snATAC-seq), on mouse cochlea at E14.5 and E16.5 in control mice and in a Ebf1 conditional knockout model.