Single cell gene expression profile of kdrl+ zebrafish cells at 48 hours post-fertilization in prdx1-morphants
We used single cell gene expression analysis to assess the effect of increased ROS (via Prdx1 knockdown) on vascular and hematopoietic progenitor populations during and after endothelial to hematopoietic transition Overall design: mCherry fluorescing cells were isolated from Tg(kdrl:mCherry) transgenic reporter zebrafish at 48 hours post-fertilization in both control and prdx1-morpholino injected embryos. The cells were analysed by single cell RNAseq