RNA-Seq analysis of Candida glabrata wild-type, ?ckb1 and ?ckb2 mutant in the presence and absence of methyl methanesulfonate (MMS).
To obtain a better understanding of molecular mechanisms underlying the DNA damage response in ?ckb1 and ?ckb2 mutants, transcriptome analyses were performed between WT, ?ckb1, and ?ckb2 strains. Comparative transcriptome analysis of ?ckb1 and ?ckb2 mutants showed similar transcriptional profiles in the presence and absence of MMS. The data also indicated that compared with WT, about 200 genes were up or downregulated in ?ckb1 and ?ckb2 strains with or without treatment with 0.01% MMS. GO analysis highlighted that the differentially expressed genes in ?ckb1 and ?ckb2 were significantly enriched in the processes related to the programmed formation of DNA double-strand breaks (DSBs), recombination, and DNA repair coordination of the meiotic cell cycle. Overall design: The study was designed to profile the transcriptomes of C. glabrata wild-type, ?ckb1 and ?ckb2 mutant grown under normal (YPD medium) and DNA damage stress (0.01% MMS treatment for 1h) conditions via RNA-Sequencing approach. The experiment was performed with three biological replicates.