Deconvolute the intermediates structural heterogeneity from the RNA ensembles using Nanopore sequencing Genome sequencing
RNA structural heterogeneity complicates the analysis of RNA structurome. In this study, we propose a novel method called nanoDISH, which utilizes Nanopore sequencing to unravel the heterogeneity of intermediate RNA structures within RNA ensembles. By separating the reads obtained from individual intermediates, we employ a multivariate SVM model to identify the modified bases. The modification profiles of each read are then transformed into bit-vectors and classified into distinct clusters, enabling the determination of alternative conformations.