Long read cDNA sequencing of barley accessions carrying Mla3 and Mla23
To understand the NLR composition at the Mla locus in barley accessions Baronesse and S13 that carry Mla3 and Mla23, respectively, we performed long read sequencing (Oxford Nanopore Technology) using a cDNA-PCR approach. Raw reads were trimmed using Porechop (v0.2.4) with default parameters.. De novo transcriptome assembly was performed using RNA-Bloom (v2.0.1) with default parameters.