Transcriptomic Expression Profile Changes in Lung Tissue of Asthmatic Mice
Asthma is a chronic inflammatory airway disease characterized by airway hyperresponsiveness and remodeling. In this study, we aimed to investigate the transcriptomic changes in the lung tissue of asthmatic mice using a well-established ovalbumin (OVA)-induced asthma model. Female BALB/c mice (6-8 weeks old) were randomly divided into two groups: sham (n=6) and OVA (n=5). The OVA group received intraperitoneal injections of 20 µg OVA and 2 mg Al(OH)3 on days 0 and 14. Subsequently, from day 21 to day 23, the mice were exposed to 1% OVA aerosol for 30 minutes each day. The sham group received an equivalent volume of vehicle. After 24 hours of the last aerosol exposure, the mice were sacrificed, and lung tissues were collected for RNA sequencing. The sequencing data were analyzed to identify differentially expressed genes and pathways associated with asthma development and progression. The results provide valuable insights into the molecular mechanisms underlying OVA-induced asthma and may identify potential therapeutic targets. Overall design: RNA-seq profiling of lung tissue from sham and OVA-challenged mice.