Spatial transcriptomics of en bloc TURBT specimens from bladder cancer patients
Bladder cancer is the ninth most common cancer worldwide, and transurethral resection of bladder tumor (TURBT) is the standard method for diagnosis and initial treatment. Recently, en bloc transurethral resection of bladder tumor (ERBT), which excises the tumor in a single piece along with the detrusor muscle, has gained attention. We performed spatial transcriptomics using the 10x Genomics Visium CytAssist platform and analyzed seven cases of pT1 high-grade non-muscle-invasive bladder cancer, focusing on the tumor boundary to characterize the invasive front. By using ERBT specimens that preserve the layered architecture, this project aims to improve our understanding of the spatial organization and molecular features of tumor invasion.