RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)
Analysis of the global variability of gene expression between the Eif6 WT control genotype and the Eif6 S235A mutated MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. RNA-seq analyses were performed on samples collected at the time of tamoxifen addition (T0), 48 hours (T1), and 72 hours (T2) after the treatment.