CRISPR screen to identify genes that contribute to type I interferon restriction of Zika virus
We performed CRISPR screens to determine human genes that contribute to type I interferon restriction of Zika virus in A549 cells. We used the PIKA CRISPR library (Ohainle et al., eLife 2018). All conditions included a 24-hour pre-treatment with 1000U/mL of IFN-beta. Cells were then infected with ZIKV (PRVABC59) at MOI=3 or left uninfected as the control condition. Dead/dying cells were harvested at 42 hours post infection by collecting the cell supernatant. Two technical replicates were performed for both the uninfected and infected conditions, and we performed the screen twice, resulting in 8 total samples.