polyA selected RNA-seq of wild-type human iPSC-derived cortical neurons upon cooling and rewarming
We aim to profile the transcriptomic changes in neurons when responding to cooling, in order to understand the neuroprotective effects of hypothermia. polyA selected RNA-seq were performed from human iPSC-derived cortical neurons under control (37 ºC), cooled (72 h at 32 ºC, day 15-18) and rewarmed (72 h at 32 ºC, day 15-18, followed by 72 h at 37 ºC, day 18-21) conditions.