A pilot messenger RNA sequencing using the blood-brain barrier in vitro model and lung cancer cell lines
Metastatic brain tumors associated with poor prognosis and limited treatment options. The blood-brain barrier (BBB) is supposed to play a major role in brain metastasis. This experiments aimed to reveal the expression profile of interaction between pericytes, endothelial cells, and cancer cells. We established an in vitro BBB model with rat primary cultured BBB-related cells (endothelial cells and pericytes), performed the gene expression of pericytes under the lung cancer cells (KNS-62 and A549) coculture circumstances. Pericytes showed inhibition of the cancer cell proliferation significantly (P<0.05). RNA was extracted from the pericytes, complementary DNA library was prepared, and RNA-seq was performed.We used three lots of biologically different of pericytes.