Single-cell transcriptomic analysis of FADS on HCC microenvironment (PRJCA041299)
To comprehensively characterize the impact of tumor cell FAD synthase (FADS) on the hepatocellular carcinoma (HCC) tumor microenvironment, we established orthotopic tumors in C57BL/6 mice using Hepa1-6 cells transfected with either shFlad1 or a control vector and performed single-cell RNA sequencing (scRNA-seq). In total, 10 samples were analyzed: 5 tumors with FADS knockdown (KD) and 5 control tumors (NC).