Mechanisms of gut epithelial barrier impairment caused by food emulsifiers polysorbate 20 and polysorbate 80
We investigated the effects of polysorbate 20 and polysorbate 80 on cytotoxicity, barrier function and the transcriptome in gastrointestinal epithelial cells. RNA-seq was used for checking transcriptome. Overall design: Monolayered Caco-2 cells were treated with polysorbate 20 and polysorbate 80 at 0.05% concentration for 24 hours. Then, RNA samples were isolated, RNA-seq performed and comparative gene expression analysis done.