Bulk RNAseq data of white matter tissue injury
We compared the transcriptomic responses in the mouse white matter tissue drive white matter lesions following LPC- and L-NIO-induced injury. Overall design: 1.Bulk RNA sequencing: The injured and contralateral tissues were isolated from the corpus callosum at 4 days after LPC-induced repair injury or L-NIO-induced WMS. The corpus callosum tissue collected from the sham-operated mice served as a control. In brief, total RNA was extracted via TRIzol reagent and quantified, after which ribosomal RNA depletion and poly(A) purification were performed. Libraries were quantified and pooled for paired-end sequencing on an Illumina NovaSeq 6000 platform. 2.Single-cell RNA sequencing: The injured and contralateral tissues were isolated from the corpus callosum at 4 days after LPC-induced repair injury or L-NIO-induced WMS. The corpus callosum tissue collected from the sham-operated mice served as a control. The tissue samples were sent for single-cell high-throughput sequencing (scRNA-seq) via the 10× Genomics platform. Briefly, viable single-cell suspensions (> 90% viability) were loaded onto microfluidic chips to generate gel bead-in-emulsions (GEMs), where cellular mRNA was barcoded and reverse transcribed. Amplified cDNA underwent fragmentation, adapter ligation, and library construction according to the manufacturer's protocol. High-throughput sequencing was performed via the Illumina NovaSeq 6000 platform to obtain high-quality transcriptome data.