The P-body protein 4E-T regulates the balance between cell genesis and establishment of the postnatal neural stem cell pool by repressing mRNA translation [Cpeb4_neurosphere]
To investigate the targets of Cpeb4/Cpeb4-associated transcripts in postnatal V-SVZ precursors cultured as neurospheres We performed RNA immunoprecipitations (RIPseq) on neurospheres using anti-Cpeb4 or control anti-IgG antibodies, isolated and sequenced the resulting RNA Overall design: Neurospheres were grown for 7 days, then cells were lysed and immunoprecipitions with either anti-Cpeb4 or anti-IgG were performed, RNA was isolated and sequenced