16S amplicon sequencing of 56 in vitro rumen fermentation fluid samples
The two Pichia strains (P. membranifaciens M12 and P. kudriavzevii Y4) were isolated from the rumen environment of Holstein cows.The total volume of each in vitro fermentation system was 50 mL. Each fermentation bottle contained 500 mg of substrate with a concentrate-to-forage ratio of 6:4. Two incubation time pointsand five experimental treatments were established