Effect of knockdown of KAT7 on gene expression in HCT116 cell line.
Accurate centriole formation is critical for cell replication. However, the holistic transcriptional regulatory mechanisms involved in this process remain elusive.We report here that KAT7 histone acetyltransferase is a key regulatory molecule that regulates centriole replication. Knockdown of KAT7 leads to reduced levels of histone H3K14 acetylation and reduced enrichment of H3K14ac in the promoter region of centriole formation-related genes, resulting in blocked centriole formation. Our data provide a new insight for the role of histone modifying factors in centriole replication-related pathways. Overall design: To investigate the role of KAT7 in gene transcription, we transfected non-specific siRNA and KAT siRNA into HCT116 cells, respectively.We then performed gene expression profiling analysis and comparative gene expression profiling analysis using data from RNA-seq of KAT knockdown and control.