Identification of responsible genes for cold tolerance mutant.
Nematode C. elegans mutant strains show cold tolerance or temperature acclimation abnormalities. bc3-18 and bc2-18 show abnormal cold acclimation. bc3-13 show abnormal cold acclimation. bc4-18 and bc4-19 show abnormal cold tolerance. To identify the responsible gene for these mutant phenotypes, recombinants were isolated by crossing N2 strains. We have mapped the responsible genes generating cold tolerance and temperature acclimation abnormalities by next-generation DNA sequencing and SNP analysis of the recombinants.