Exome and multi-site bulk and scRNA-seq profiling of pleural mesothelioma
This study comprehensively profiles pleural mesothelioma (PM) using integrated bulk DNA sequencing [whole-exome sequencing (WES)], bulk RNA-sequencing (RNA-seq), single-cell RNA-sequencing (scRNA-seq), optical genome mapping, spatial transcriptomics, and spatial proteomics. The goals are to characterize malignant and stromal cell states, identify malignant phenotypes, define tumor microenvironmental correlates, and evaluate associations with clinical outcomes and survival.Primary ObjectivesMap malignant cell programs and epithelial_mesenchymal gradients across anatomic sites. Define stromal and immune microenvironment states using scRNA-seq and spatial assays. Integrate multi-omic data to identify structural variants, CNVs, SNVs, and transcriptomic programs to determine relationship... (for more see dbGaP study page.)