RNAseq of primary osteoblasts isolated from Prx1-CreWT HuRfl/fl and Prx1Cre+/- HuRfl/fl mouse long bone
Primary osteoblasts were cultured from long bones of Prx1-CreWT HuRfl/fl (Control) and Prx1Cre+/- HuRfl/fl (Developing limb mesenchyme knockout of HuR) mice. Total RNA was extracted from these cells using Trizol method and RNA-Seq used to detect differential gene expression.