Gallus gallus breed:Rhode Island Red Raw sequence reads
Eggshell strength is a critical economic trait that declines with hen age, yet the molecular mechanisms distinguishing stable genetic determinants from age-responsive pathways remain unclear. This study implemented a multi-stage comparative framework using uterine transcriptomes from Rhode Island Red hens at peak lay (60 weeks) and late lay (90 weeks) to address this question. At each age, hens were stratified into Weak and Strong shell strength groups, with 5 individuals per group selected for RNA-seq analysis.