50:50 mixture of differentiated THP-1 macrophages and K562 cells treated with 1µg/ml of LPS, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags
The purpose of this experiment was to observe secondary signaling response of THP-1 to K562 cells observed in real time with ChronoSeq. Cells were sampled every 15 minutes. LPS was added 5 minutes after the first sample. Watchmaker RNAase inhibitor of 800U/µl was added to all Time-Tagged beads for a final concentration of 3U/µl, because of high RNAase activity in THP-1 supernatants.