Methylation mediated LINC00261 suppresses pancreatic cancer progression
To the best of our knowledge, this is the first study presenting detailed evidences for the underlying role of LINC00261 in pancreatic cancer both in vitro and in vivo. Also, we thoroughly explored the clinical value of LINC00261 in multicenter cohorts and developed an individualized predictive model of survival. To investigate the potential downstream pathway of LINC00261, we performed RNA-seq using two PANC-1 cell groups: LINC00261 knockdown and control. Overall design: PANC-1 cells were transfected with LINC00261 siRNA and control . Total RNA was extracted from the samples using the TRIzol method. The total RNA extracted from the samples was tested by a NanoDrop 2000 and denaturing gel electrophoresis. RNA Sequencing Analysis were performed after the sample quality control.