Triticum turgidum strain:Kronos Transcriptome or Gene expression
For this project, we have sequenced, assembled and annotated a transcriptome of a tetraploid wheat T. turgidum cv. Kronos. The sequencing libraries were prepared from root and shoot tissues from young 2-3 week old seedlings, developing spikes at the booting stage and grain 20 days post anthesis. An additional library from multiple tissues was normalized with double-stranded DNA nuclease. All sequencing was carried out on the Illumina HiSeq platform using the 100 bp paired-end protocol (562.4 million reads). The assembly was constructed using a multiple k-mer approach with a de novo assembly algorithm implemented in CLC Genomics Workbench 5.5 and additional redundancy reduction with CD-HIT and blast2cap3 programs. Open reading frames and proteins were predicted using BLASTX searches and a findorf algorithm.