High-fat Diets Single-Cell RNA-seq Dataset
Here, we present an atlas of the impact of seven distinct high-fat diets on murine host physiology over the course of a year as read out in fecal metabolomics, fecal and serum lipidomics, and host single-cell transcriptomics. Additionally, we present fecal metagenomics at six timepoints during the year to evaluate the impact of the diets on the gut microbiome. This study features reversals to the control diet for each high-fat diet after four and nine months. This positions it uniquely to interrogate the changes in the microbiota, metabolome, and host response to high fat diets even after the mice are reverted to the control diet for a span of months. Additional unique features of this study include the assessment of the impact of baseline microbiome on response, integration of host single cell transcriptomics with metagenomics of the microbiota, integration of the fecal metabolome with the microbiome, and the ability to compare all of these measurements and analyses across diets. This extensive resource is available in an RShiny app.