Effect of 2 mM and 17 mM 7-day intermittent EtOH exposure on human excitatory neurons
To investigate the impact of EtOH exposure on human excitatory neurons, we induced NGN2 expression in 4 neural progenitor cell lines. We then conducted a 7-day intermittent EtOH exposure protocol starting at Day 21 post-induction using two concentrations: 2 mM (0.01 BAC equivalent) and 17 mM (0.08 BAC equivalent). RNA was harvested at Day 28 post induction. Overall design: Comparative gene expression profiling analysis of bulk RNA-seq data for NGN2 neurons treated with 0 mM, 2mM, or 17mM EtOH