Transcriptome of HaCaT cells treated with Loxosceles venoms and recombinant SMase D in the presence or absence of tetracycline
Loxosceles spider venoms are complex mixtures of bioactive components that induce a wide range of toxic effects in human skin, including inflammation and dermonecrosis. Among these components, sphingomyelinases D (SMases D) are the main toxins responsible for the local and systemic manifestations of loxoscelism, as they trigger cellular damage, cytokine release, and activation of signaling pathways associated with immune response and extracellular matrix degradation. Despite their biological relevance, the global transcriptional responses induced by Loxosceles venoms and distinct classes of SMases D in human keratinocytes remain incompletely understood. Here, we performed RNA sequencing (RNA-seq) to investigate gene expression changes in HaCaT human keratinocytes exposed to Loxosceles intermedia and Loxosceles laeta venoms, as well as recombinant sphingomyelinase D Class I and Class II. Cells were cultured under serum-free conditions and treated with venoms (10 ug/mL) or recombinant toxins (5 ug/mL) for 2 hours at 37oC in DMEM medium supplemented with VBS++ buffer. Treatments were performed in the presence or absence of tetracycline (50 ug/mL), a compound known for its anti-inflammatory properties and inhibitory effects on matrix metalloproteinases. Control samples consisted of PBS-treated cells with or without tetracycline. Taken together, this dataset provides a comprehensive resource for understanding the molecular mechanisms underlying keratinocyte responses to Loxosceles venoms and SMases D, as well as the modulatory effects of tetracycline. These data contribute to the identification of key pathways involved in loxoscelism and may support the development of novel therapeutic strategies.