Principles of chromosome organization for meiotic recombination
In meiotic cells, chromosomes are organized as chromatin loop arrays anchored to a protein axis. This organization is essential to regulate meiotic recombination, from DNA double-strand break (DSB) formation to their repair. In mammals, it is unknown how chromatin loops are organized along the genome and how proteins participating in DSB formation are tethered to the chromosome axes. Here, we identified three categories of axis-associated genomic sites: PRDM9 binding sites, where DSBs form, binding sites of the insulator protein CTCF, and H3K4me3-enriched sites. We demonstrated that PRDM9 promotes the recruitment of MEI4 and IHO1, two proteins essential for DSB formation. In turn, IHO1 anchors DSB sites to the axis components HORMAD1 and SYCP3. We discovered that IHO1, HORMAD1 and SYCP3 are associated at the DSB ends during DSB repair. Our results highlight how interactions of proteins with specific genomic elements shape the meiotic chromosome organization for recombination. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for PRDM9, MEI4, IHO1, HORMAD1, SYCP3 and CTCF proteins in differents genetic backgrounds in testes enriched in Leptotene-Zygotene spematocytes Please note that no processed data is provided for the following knockout samples as all processed data have been subtracted by its corresponding knockout (as detailed in the sample data processing field): Prdm9 ChIP-seq of the Prdm9KO mouse MEI4 ChIP-seq of the Mei4KO mouse IHO1 ChIP-seq of the Iho1KO mouse HORMAD1 ChIP-seq of the Hormad1KO mouse SYCP3 ChIP-seq of the Sycp3KO mouse