Vincristine induces spermatogenic impairment in a B-ALL PDX mouse model via downregulation of Tssk4 and Ccdc159: RNA-seq transcriptomic profiling
This dataset contains RNA sequencing (RNA-seq) data generated from testicular tissues of a B-cell acute lymphoblastic leukemia (B-ALL) patient-derived xenograft (PDX) mouse model treated with vincristine (VCR). The study aimed to elucidate the molecular mechanisms underlying VCR-induced reproductive toxicity. Samples were collected from multiple experimental groups, including normal controls (NC), leukemia-bearing controls (ALL), and VCR-treated groups with varying leukemia infiltration levels. Transcriptomic analysis revealed widespread gene expression perturbations following VCR exposure, with significant downregulation of genes crucial for spermatogenesis and sperm flagellar assembly. This data provides a comprehensive resource for understanding the genetic pathways affected by VCR in the testicular microenvironment. Overall design: RNA-seq profiling of testicular tissues from five experimental groups of PDX mice: Normal control (NC), B-ALL model control (ALL), VCR-only treated, and VCR-treated with low (Low-VCR) and high (High-VCR) peripheral blood leukemia infiltration.