RNAseq experiment to identify differentially expressed genes by AP2 induction in the Shoot Apical Meristem (SAM) of Arabidopsis
Trough a two components inducible system, we expressed ectopically in the SAM a resistant version of AP2 to the regulation of miR172 (AP2m3).  The differentially expressed genes by AP2 induction allows the identification of differents groups of genes that could mediate AP2 functions in the SAM. Overall design: Two weeks after bolting, SAM of Arabidopsis plants were treated with dexamethasone, that allows the AP2m3 expression, or mock. 6-8 hours after treatment SAM were collected removing all visible flower primordia. RNA was extracted and sequenced on a MiSeq (Illumina),