RNA-seq analysis of BW25113 Escherichia coli (Wild-type, and mutants lacking the glucose metabolism regulator TmaR or the RNA Chaperone Hfq) experiencing long-term nitrogen starvation in Gutnick minimal media
RNA-seq was carried out to compare the transcriptomes of wild-type BW25113 E coli with mutants lacking either the glucose metabolism regulator TmaR or the ubiquitous RNA chaperone Hfq, in bacteria that had been exposed to long-term (24hr) nitrogen starvation in Gutnick minimal media. All strains also contain the empty plasmid pBAD18, as this was initial performed alongside a larger RNA-seq study - as such the WT and Hfq mutant are previously available in E-MTAB-14054. TmaR is also required for the formation of Hfq foci, a novel sub-cellular feature that forms during long-term nitrogen starvation. The aim of this work was to use the mutant lacking TmaR to understand the functional role of the Hfq foci during long-term nitrogen starvation.