Transcriptional profiles of venous and lymphatic endothelial cells from tie1 mutant zebrafish
To investigate what kind of gene expression is regulated downstream of Tie1 signaling, we performed RNA-seq analyses on venous and lymphatic endothelial cells (ECs) between homozygous tie1 mutant embryos and their wild-type and heterozygous siblings during secondary sprouting, respectively. Our analyses indicate that Tie1 signaling controls a series of gene expression involved in lymphatic development including EC migration, proliferation, and differentiation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for endothelial cells from homozygous tie1 mutant embryos and their wild-type and heterozygous siblings in zebrafish.