CPSF6 regulates alternative polyadenylation and proliferation of cancer cell through phase separation
In order to explore the impact of phase separation of CPSF6 on APA, we identified the structural domains that affect its phase separation formation and performed mutations. In addition, we also found that the phosphorylation of CPSF6 can also affect its phase separation effect, and identified key kinases. We performed IVT-SAPAS sequencing on both cases mentioned above. Overall design: Compare the changes in APA length between IVT-SAPAS groups