scRNA-seq analysis of murine TECs after depleting RANKL and CD40L signaling
the molecular mechanisms behind the turnover of medullry thymic epithelial cells remain unclear.  We performeds single cell RNA-seq of TECs from mice 15 days after depleting cytokine RANKL and CD40L signaling. Overall design: Thymic epithelial cells (EPCAM+, CD45-, reticulocyte negative ) were isolated from the thymus of wild-type and CD40-deficient mice after injection of RANKL neutralizing antibody