YAP-Driven Malignant Reprogramming of Oral Epithelial Stem Cells, CUT&TAG sequencing for YAP, H3K27ac, and H3K27me3
Cleavave Under Targets and Tagmentation (CUT&TAG) sequencing for YAP, H3K27ac, and H3K27me3 of tongue epithelia-derived, transgene-selected primarily cultured cells from control mice or transgenic mice expressing HPV16E6-E7 and YAP1S127A Overall design: Fifteen days after transgene induction, cells were isolated from whole tongue epithelia, sorted for GFP expression, cultured, then subjected to CUT&TAGseq, library preparation, and sequencing on Illumina NovaSeq6000.