Transcriptome analysis after BBB-EC migration
A monolayer of hCMEC/D3 (BBB-EC) was grown in an insert. After reaching confluency, BBB-EC were treated with TNFa and IFNg for 24h. Next, BBB-EC were washed. Tregs were isolated from human blood (both healthy donors (HD)and untreated RRMS patients) and added to the upper chamber of the insert or were cultured in the BBB-EC medium as control (untouched_1). They were let to migrate for 24h and the upper fraction (non-migrated_2) and lower fraction (migrated_3) were collected. Overall design: Comparative gene expression profiling analysis of RNA-seq data of untouched, non-migrated and migrated Tregs from healthy donors and untreated RRMS patients.