RNA sequencing of tumor-infiltrating regulatory T cells with or without Glud1 knockout
By crossing Foxp3-YFP-cre mice with Glud1 fl/fl mice, we specifically knocked out the expression of Glud1 in Treg cells of the mice. Through RNA sequencing analysis of splenic and tumor-ifiltratingTreg cells, we investigated the differential expression genes in Treg cells under different conditions.