S. aureus agr-type transcriptomes
The Staphylococcus aureus Newman strain was previously engineered by deleting its native agr locus (?agr) and chromosomally complementing it with each of the four agr alleles (agr-I, agr-II, agr-III, and agr-IV), by Geisinger et al. (Journal of Bacteriology, 2012). The resulting congenic panel—comprising the four complemented strains and the ?agr mutant—was first subcultured three times to avoid any AIP accumulation and agr-activation, and then cultured 12 h in rich medium and sampled at three growth stages: early exponential phase (1.5 h), late exponential phase (6 h), and stationary phase (12 h). RNA-seq was performed for all strains across all time points to assess agr-type–specific transcriptional profiles