RNA-seq, G pallida population D383 batch 1-2A cyst and hatched J2 samples, exposed for 8h, 24h, 48h to 2nM Solanoeclepin A (dissolved in 1% EtOH), potato root diffusate from wild accession PNT 777-2 (diluted to 0,1x with tap water) or tap water with 1% EtOH (spontaneous hatching control)
In this experiment we evaluate the transcriptional responses of G pallida D383 1-2A to diverse hatching stimulations by pure Solanoeclepin A or by potato root exudate. We used cysts hydrated for 7 days in tap water for the experiment. Water for hydration was changed every day. After hydration approximately 13mg of cysts (50-100 cysts) were placed over Netwell inserts (Corning) with 74um mesh size. For this experiment we used 5 “technical” replicates, all performed in the same experiment. Worms underwent the treatment at the Laboratory of Nematology (WUR)(Spit lab) and RNA was extracted in the same lab using the Qiagen RNeasy Micro kit. RNA samples were measured by Nanodrop and Qubit and were sent to the Wageningen BioInformatics business unit. (Wageningen, The Netherlands, Dr. Sara Diaz Trivino's team) on dry ice. Sequencing was performed by the DLO in Wageningen.