Role of m6A-Modified GAS6 in Rheumatoid Arthritis: Insights from Multi-Omics Analysis
Synovial tissue samples were collected from RA and HC were used for m6A seq and RNA seq analysis. Peripheral blood mononuclear cells (PBMCs) were isolated from RA and HC. The mRNA expression of GAS6 and MERTK was quantified by qRT-PCR, and inflammatory cytokine (IL-1ß, IL-6, IL-10, and TGF-ß1) were detected by ELISA. GAS6, MERTK were knocked down in RA-FLS using siRNA interference. CCK-8 were used to assess cell viability, Flow cytometry to assess apoptosis , and Cell scratch assay to evaluate cell migration rate. Immunofluorescence and Western blott was used to determine protein localization and expression. Overall design: Synovial tissue samples were collected from RA and HC were used for RNA seq analysis.