The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia [CITE-Seq]
Single-cell RNA-seq analysis of primary human bone marrow immune populations isolated from B-ALL patient samples (matched diagnosis, remission and relapse), and healthy bone marrow donor samples. Overall design: Single-cell RNA-sequencing libraries of primary human bone marrow immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq.