Exploring the Mechanism of Massage on Ulcerative Colitis Model Mice Based on the Balance of helper T and regulatory T Cells
In this study, we aim to investigate the effects and mechanisms of treatment with massage on mice with dextran sulfate sodium (DSS) induced ulcerative colitis.Balb/c mice will be randomly divided into control, model, mesalazine, and massage groups. The UC model will be established by 2.5%DSS in the model, mesalazine, and massage groups.After the DSS administration, the mesalazine group will receive mesalazine solution once daily for 7 days, following the equivalent conversion formula for humans and mice. The massage group will undergo massage for 10 min daily for 7 consecutive days.The disease activity index score for each group will be evaluated based on their health status from the previous day. HE staining will be used to observe the pathological changes of the colon. Array analysis for cytokines will be used to screen the related inflammatory factors and pathways.RNA-seq technology will be used to detect differentially expressed genes and screen for related signaling pathways. Flow cytometry and immunofluorescence staining will be used to observe the proportion of T cell subsets.RT-PCR and western blotting will be used to detect the expressions of T cell-related cytokines and transcription factors. We will comprehensively analyze the above results and explore the mechanism of massage therapy for ulcerative colitis.