Small non-coding RNA sequencing of human monocyte-derived macrophages
The goal of this experiment was to identify the differential expression of microRNAs in human polarised macrophages. We used monocyte-derived macrophages isolated from 8 different healthy invididuals. Differentiated macrophages were polarised towards the pro-inflammatory M1-like phenotype, by using LPS and INF-??. Total RNA was extracted and sent for small-RNA sequencing to the company Novogene. Overall design: Identification of microRNAs differentially expressed between unpolarised and M1 polarised macrophages