RNA-EC-TEM-1-AC
This study investigates the transcriptomic and genomic responses of Escherichia coli clinical isolates to pharmacologically relevant concentrations of amoxicillin-clavulanic acid (AMX-CLV). Using a hollow-fibre infection model to reproduce human plasma and urinary drug pharmacokinetics, we performed RNA sequencing to characterise differential gene expression patterns in drug-sensitive and drug-selected resistant subpopulations. The dataset includes RNA-seq profiles generated from isolates sampled during time-course experiments under distinct AMX-CLV exposure conditions that correspond to clinically achievable systemic drug concentrations. These data support the analysis of ß-lactam/ß-lactamase inhibitor responses, stress-adaptive pathways, mobilisation of mobile genetic elements, and mechanisms associated with resistance emergence.