Noncoding RNA profiling in omentum adipose tissue from obese patients and the identification of novel metabolic biomarkers
Obesity, a prevalent metabolic disorder, is linked to perturbations in the balance of gene expression regulation. Noncoding RNAs (ncRNAs), including long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs), play pivotal roles in regulating gene expression. The aim of this study was to identify additional ncRNA candidates that are implicated in obesity, elucidating their potential as key regulators of the pathogenesis of obesity. In our research, compared with those in healthy individuals, various lncRNAs, circRNAs, and miRNAs were significantly differentially expressed in obese subjects. We established comprehensive ncRNA profiles from human greater omentum adipose tissue and compared the profiles between obese individuals with healthy controls via whole-transcriptome sequencing. Subsequent validation via qRT?PCR substantiated the sequencing results, reinforcing the reliability of our findings. These insights not only validate the importance of ncRNAs but also lay a theoretical foundation for further functional exploration of the roles of lncRNAs, circRNAs, and miRNAs in obesity, potentially offering novel perspectives on the pathogenesis of this condition. Overall design: We detected differentially expressed ncRNAs in the greater omentum of obese and healthy individuals by whole transcriptome sequencing. Then we performed GO analysis, KEGG pathway analysis, real-time quantitative polymerase chain reaction (qRT-PCR), Protein-protein interaction (PPI) and hub gene analysis.