Next Generation Sequencing analysis using SPOP mutant (F133V)-transduced RM-1-BM mouse prostate cancer cells and RAW264.7 mouse macrophage cells
In this experiment, we analyzed the gene expression profiles of SPOP mutant (F133V)-transduced (and vector control) RM-1-BM mouse prostate cancer cells and RAW264.7 macrophage cells in transwell coculture and monoculture in the context of olaparib (or vehicle) treatment by RNA sequencing. Overall design: Fresh SPOP mutant (F133V)- and vector control-transduced RM-1-BM mouse prostate cancer cell and RAW264.7 mRNA profiles from olaparib- or vehicle-treated transwell cocultures and monocultures were generated by deep sequencing, in triplicate