ac4C-RIP-seq to identify mRNA ac4C peaks
NAT10-catalyzed N4-acetylcytidine (ac4C) has emerged as a vital post-transcriptional modulator on the coding transcriptome by promoting mRNA stability. To explore the transcriptome-wide profile of ac4C modification, we mapped the locations of ac4C modification on wild-type (WT) hESCs and NAT10 KD hESCs by high-throughput ac4C RNA immunoprecipitation sequencing (ac4C-RIP-seq). Overall design: Both NAT10 KD hESCs and wild-type (WT) hESCs were analyzed with 2 duplications for ac4C-RIP-seq.