ChIP-seq of H3K27ac in Control and NCoR1 KD CD8a+ DCs at 0hr, 2hr and 6hr of TLR stimulation
H3K27ac ChIP-seq was performed to identify active enhancers in different TLR activated control CD8a+ DCs as well as to understand the role of NCoR1 in regulating TLR specific enhacner activity using H3K27ac ChIP-seq performed in NCoR1 KD DCs. MutuDC1940 cell line were treated with TLR3 ligand poly I:C, TLR9 ligand CpG-B and combined stimulation of TLR9 , TLR3, and IFNg for 2hr and 6hr. Recombinant Anti-Histone H3 (acetyl K27) antibody was used to pull down chromatin regions having active enhacner mark in each unstimulated and stimulated condition.