Preservation temperature of salivary microbiota in 20 human adult samples
Saliva samples were collected from 5 human healthy adults . Each sample was devided  into 4 alliquots and stored at different temperature conditions. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R) and, multiplexed amplicon pyrosequencing was carried out using Illumina MiSeq.