Functionally impaired plasmacytoid dendritic cells and non-haematopoietic sources of type I interferon dominate human autoimmunity
We purified pDCs from healthy controls (n = 8), At-Risk individuals (n = 4) and SLE patients (n = 13) by negative selection then sorted the cells to achieve purity >99% based on CD304 (BDCA-4) expression and sequenced their RNA using Smart-seq2 libraries to determine differentially expressed transcripts.