High-resolution HLA typing by NGS reveals novel alleles in the Amazonian population
This study aimed to characterize the HLA allelic diversity in the population of Amazonas and to implement the NGS-based typing protocol at the Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas (HEMOAM), following national guidelines. DNA was extracted from whole blood samples of volunteer bone marrow donors, recipients treated at the HEMOAM hospital, and related donors using the PureLink DNA kit (Invitrogen). Library preparation was performed with the AllType FastPlex kit (OneLambda), followed by sequencing on the Illumina MiSeq platform.