Gene expression analysis of hepatocyte-specific Taf2 conditional knockout mice
Taf2 is a transcription co-factor and a member of TFIID complex. To better understand gene regulation of Taf2 we generated a hepatocyte-specific Taf2 conditional knockout mouse. RNA-seq was performed in hepatocytes isolated from these mice as well as WT mice. It shows that Taf2 deletion in hepatocytes induces cell death and compensatory proliferation which contributes to inflammation and fibrosis in the liver. Overall design: Hepatocytes were isolated from WT and hepatocyte-specific Taf2 conditional knockout mice. Two days after plating the cells were harvested, RNA was extracted and subjected to RNA-seq analysis.