A new nul DPB1*01 allele
Here we describe a novel nul DPB1*01 allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (NG-MIX kit, UPR, RENNES , France) and  Oxford Nanopore sequencing technology. Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or P2 Solo platforms . Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by nanotyper  software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following:  A*03:01 ,*68:02 B*07:02 ,*51:01 C*07:02 ,*16:01 DRB1*04:04, *11:02 DRB3*02:02 DRB4*01:01P DQA1*03:01 ,*05:05 DQB1*03:02 ,*03:19 DPA1*01:03 ,*02:01 DPB1*01 :New, *06 :01 HLA-DPB1*01 : New allele differs from DPB1*01 :01 by one nucleotide deletion at position 18 of exon 5 (Position 774 in cDNA), where  base T  is deleted .This mutation is located in th STOP codon , which could induce a translation of an abnormal protein that can not be expressed  on th celle surface