Single-cell RNA-seq data of large-scale bone calluses from skeletally immature and mature mice
Age and skeletal maturity impacts the capacity for bone regeneration. Using a mouse model of large-scale rib resection, we demonstrated that skeletally immature mice regenerate the rib around 14 days post resection, whereas skeletally mature mice never fully regenerate the rib. Here, we performed single-cell RNA sequencing analysis of rib calluses from skeletally immature and mature mice at 5 and 14 days post-resection to characterize the age-related changes in cell population frequencies and their gene expression profiles during the early and late stages repair of bone repair. Overall design: Rib calluses from 4 young skeletally immature and 4 old mature mice at 5 and 14 days post lage-scale rib resection were used in these studies. Repair rib calluses were enzymatically dissociated and analyzed by scRNAseq using 10x Genomics platform. NIDCD/NIDCR Genomics and Computational Biology Core