RNA-seq analysis of NCI-N87 cells following lentivirus-mediated knockdown of ASF1B
NCI-N87 cells, with or without ASF1B knockdown, were lysed using TRIzol reagent (Invitrogen, 15596026). Total RNA was isolated using the TRIzol-based RNA extraction method. The global gene expression profiles were detected by mRNA sequencing on the Illumina HiSeq Xten sequencing platform (Basebio, Chengdu, China).