Transcriptomes of Agrobacterium chromosome architecture variants
To elucidate the impact of chromosome architecture on transcription, we generated isogenic Agrobacterium strains that only differ in chromosome configurations. We chose A. fabrum C58 and C58F, to generate a set of four strains with different chromosome configurations: C58 Wildtype (WT, Circular + Linear), C58 Circularized (C58Cir, Circular + Circular), C58F (FL, Linear), and C58F Circularized (FCir, Circular). Samples from each strain were grown in triplicate in two conditions, acetosyringone (AS) and control solvent (DMSO). RNAseq was performed on samples from each condition and gene expression was measured in the AS samples relative to its DMSO control.