Transcriptomic Analysis of Colletotrichum camelliae JX01 in Response to Pseudomonas silvicola PSM6
Pseudomonas silvicola strain PSM6 is a biocontrol agent isolated from healthy Pinus massoniana. Previous studies have confirmed that it can significantly inhibit the growth and infection of Colletotrichum camelliae by secreting antimicrobial substances and inducing systemic resistance in the host, showing excellent potential for biocontrol applications. However, current research has mostly remained at the phenotypic level. The molecular regulatory network and key functional genes involved in PSM6-induced resistance to anthracnose in tea plants have not been systematically elucidated, and the lag in theoretical research has severely restricted the industrial application of this strain. Transcriptome sequencing (RNA-seq) enables comprehensive capture of gene expression dynamics and accurate identification of differentially expressed genes and core regulatory pathways, providing high-throughput support for clarifying the molecular mechanism underlying pathogen inhibition induced by biocontrol strain PSM6.In this project, three treatment groups were established: blank PDA medium, PDA medium mixed with PSM6 cell-free filtrate, and PDA medium mixed with PSM6 fermentation broth, all cultured for 5 days. Transcriptome sequencing was performed to screen key differentially expressed genes in C. camelliae in response to PSM6 induction. Combined with GO and KEGG enrichment analyses, core regulatory pathways were identified. The results will reveal the biocontrol molecular mechanism of strain PSM6, improve the theoretical system of the interaction between PSM6 and C. camelliae, provide gene targets and technical support for the biological control of tea anthracnose, promote the industrial development of PSM6 biocontrol agents, and contribute to the green and sustainable development of the tea industry.