Late-Acting Self-Incompatibility in Asimina triloba
Early developing seeds were collected from three cross-compatible (CC) and three auto-incompatible (AI) pollinated flowers at 4, 8 and 15 days after pollination (DAP), resulting in a total of 18 biological replicates. The pistils of each flower were dissected to obtain the developing seeds, RNA extraction from these fresh early seeds was performed using a specific protocol that combines CTAB (Doyle & Doyle, 1987, Phytochem. Bull. 19: 11–15) and the RNeasy Plant Mini Kit (Qiagen, Stanford, CA).