Identification of early glutamine-responsive genes in Arabidopsis
To understand the global effect of Gln on gene expression, we performed RNA-seq analysis to compare the gene expression profiles between Arabidopsis seedlings treated with Gln for 0hr and 0.5hr. Overall design: Total RNA extracted from 14-day-old Arabidopsis seedlings grown on liquid medium containing 1/2 MS-modified basal salt mixture without N (M531, PhytoTechnology Laboratories) and then treated with Gln for 0hr and 0.5hr was used for RNAseq analysis.