Effect of high temperature on Arabidopsis thaliana root tips
Plant roots located in the upper soil layers are prone to experience high temperatures. To gain insight into the effect of high temperature on root development and functioning, we exposed five-day-old Arabidopsis thaliana seedlings grown on agar plates to 30 °C for 48 hours, and compared the gene expression profile in the root tip with that from seedlings that remained at 22 °C. Overall design: Seedlings were grown on agar plates for 5 days at 22 °C before start of the treatment. Control treatment was 48 hours at 22 °C, high temperature treatment was 48 hours at 30 °C. At the end of the treatment, 1-mm long tips of the roots were collected. 4 biological replicates were used from each treatment. A replicate consisted of pooled tissue from 50 seedlings (grown on 5 plates, 10 seedlings/plate).