Anti-killing RNAseq
Three tumor cell lines derived from different tissues, HEK293T, RKO and MDA-MB-231, were used as research objects to establish a stable T cell-mediated tumor cell killing model. Combined with unbiased genome-wide CRISPR-A/CRISPR-KO screening and transcriptome sequencing technology, differentially expressed genes related to resistance to tumor cell immune killing were systematically screened in the immune checkpoint inhibitor-treated group and the untreated control group.