Experimental evolution of cell cycle networks
This repository contains results for the sequencing of strains resulting from experimental evolution of cells with different cell cycle networks under optimal conditions (in ministats). The sequencing was performed by Genohub.44 samples were submitted for library construction (400bp insert) and paired-end sequencing on one lane of Illumina HiSeq 2500 (150 cycles). There are 90 FastQ files, 2/sample and 2 for reads with undetermined indexes.Samples names from the experimental evolution and corresponding FastQ file IDs are in the samples_IDs.csv file in this folder.Sequenced samples correspond to single clones or populations from days 1, 35 and 70 of the experimental evolution.NAMING CONVENTION: Samples are named by ministat number (M#) and day of of the experimental evolution. Single clones are numbers after (.#) and population samples start with a p. For example:M3D35.2 is clone 2 taken from ministat 3 on day 35pM4D70 is a population samples from ministat 4, taken at day 70.Samples from day 1 (M#D1.#)are the ancestral strains.Ministats 1, 2, 3 contain strain A (defective cell cycle).Ministat 4 contains strain B (minimal cell cycle).Minstat 7 contains strain C (normal cell cycle, auxotrophy control).Ancestral strains are:A. C566: leu1::Pcdc13::cdc13Scdc2T14AY15Fas::cdc13UTR::ura4+ Dcdc2::KAN Dcdc13::NAT Dcig1::HYG Dcig2::KAN Dpuc1::leu2+ Dgsf2::KAN ura4-D18 h-B. DC561: leu1::Pcdc13::cdc13Scdc2as::cdc13UTR::ura4+ Dcdc2::KAN Dcdc13::NAT Dcig1::HYG Dcig2::KAN Dpuc1::leu2+ Dgsf2::KAN ura4-D18 h+C. DC596: ura4::leu1area leu2::puc1area Dgsf2::KAN leu1-32 ura4-D18 h-