Bone marrow macrophage RNA sequencing
Total WT and FTO KO BMDM RNAs were extracted using a TRIzol reagent. RNA quality and quantity were determined with a Nanodrop TM OneCspectrophotometer and Qubit3.0 with the Qubit TM RNA Broad Range Assay kit. Fifty micrograms of total RNA were used for polyadenylated RNA enrichment using VAHTS mRNA capture beads. For RNA seq, mRNA from each sample was used to generate the library using the Illumina kit.