In vivo CRISPR screens for Toxoplasma hyperLOPIT-unassigened proteins_2
We performed in vivo CRISPR screens of Toxoplasma targeting hyperLOPIT-unassigened genes during mouse infection. Overall design: This Unassigned_2 library targeting 295 genes which consisted 2949 gRNAs targeting hyperLOPIT-unassigened genes together with dispensable and essential control genes.