Impact of CRP deficiency on the global gene expression of Klebsiella variicola DX120E in response to nitrogen limitation
Klebsiella variicola DX120E is a plant growth-promoting endophyte capable of biological nitrogen fixation. The coordination between carbon and nitrogen metabolism is vital for its survival and efficiency in sugarcane. This project aims to investigate the regulatory role of the global transcription factor CRP (Cyclic AMP Receptor Protein) under nitrogen-starved conditions. We constructed a crp gene deletion mutant of strain DX120E and compared its gene expression profile with the wild-type strain using high-throughput RNA sequencing. The bacterial cultures were maintained in nitrogen-deficient medium to simulate nutrient-limiting stress. The resulting transcriptomic data reveals the CRP-dependent regulon, highlighting its involvement in central carbon metabolism, nitrogen assimilation, and potential cross-talk with nitrogen fixation genes in K. variicola DX120E.