Effects of the Sox17 ?50 mutation on SOX17 function in mouse embryonic liver development
To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17?50 mutant animals and surveyed how this mutation altered Sox17 expression. Livers were isolated from E12.5 Sox17?50/?50 and Sox17+/+ embryos (n = 4 of each genotype),  RNA  isolated, and bulk RNA-Seq performed.