DNA methylation profiling of adult monozygotic twins discordant for weight at birth
17 pairs of monozygotic monochorionic twins were recruited from the East Flanders Prospective Twin Survey based on severe birth-weight discordance (>30%). DNA was obtained from saliva samples. Genome-scale DNA methylation profiles were obtained on HumanMethylation450 BeadChip. 8 candidate CpG positions were selected in the association analysis and validated using deep bisulfite sequencing of surrounding amplicons on GS FLX Titanium System.