Metagenome of protein amended anaerobic marine sediment slurries of the Helgoland mud area -- placeholder
PLACEHOLDER An incubation experiment was performed, investigating protein degradation by uncultivated Thermoplasmatota in marine anaerobic sediments of the Helgoland mud area. Slurry for the first generation was prepared in a 1:4 ratio of sediment and sulfate-free artificial sea water. The used sediment originated from core HE483 station 2-2, 45-70 cm and retrieved by gravity coring in 2017.  The incubation experiment was performed anoxically:  the protein amended sample was amended with 20 mM sulfate (Na2SO4), an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin; 50 mg/l for each) and 1.86 g/l egg white protein. After 372 days 5mL of the first generation incubation was transferred into anoxic Widdel medium. The incubation experiment was performed anoxically : the protein amended samples contained with 30 mM sulfate (Na2SO4) and an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin; 40 mg/l for each) and 2.33 g/l egg white protein. Sampling of the second generation was performed on day 157 day. DNA was extracted and the metabolic potential of the archaeal community was analyzed using metagenomics.