scRNAseq mapping asexual life-cycle progression of P.berghei ANKA parasitised RBCs in vivo with and without systemic host inflammation
Red blood cells (RBCs) from Plasmodium berghei ANKA infected mice were recovered to undergo 10X Chromium, droplet-based sequencing. Additionally, scRNAseq was performed on parasitised RBCs in the presence of systemic host inflammation induced by acute parasite infection or LPS conditioning. These analyses were performed to help identify genes or pathways that may contribute to the parasite maturation defects induced by systemic host inflammation.