RNA-seq of human neuroblastoma cell line (SH-SY5Y) treated with Branaplam and after compound washout.
We performed a compound washout experiment to assess the reversibility of events triggered by Branaplam. We treated SH-SY5Y cells with 100 nM Branaplam for 24 hours, washed out the treatment and carried out RNA-seq after a further 48 hours incubation in cell culture medium.