RNA-seq right ventricle congenital heart disease paediatric patients
RV samples were collected from discarded myocardial tissue from paediatric patients under 16 years of age undergoing their first cardiac surgery at Bristol Royal Hospital for Children. Fresh biopsies (10 mg net weight) were collected from surgery from the apex of the RV immediately after institution of cardiac pulmonary bypass and snap frozen upon harvesting. Strand-specific bulk RNA sequencing was performed on whole tissue snap frozen RV biopsies via Genewiz (Azenta). Briefly, total RNA from RV was isolated from 10mg of tissue, with poly-A selection. Sequencing was conducted on Illumina NovaSeq, yielding 2x150bp paired end reads, 30 million read pairs per sample, with >80% of bases with Q30 or higher. Overall design: To investigate the RNA profile of right ventricle in congenital heart diesease patients taken during their first corrective surgery, RNA sequencing was conducted.