Dynamic evolution of TCF3-PBX1 leukemias at the single-cell level under chemotherapy pressure [scRNA-seq]
We have developed a mouse model in order to characterize in vivo chemotherapy resistance depending on niche. Global transcriptomics and phospho-proteomics at the single-cell level elucidated novel mechanisms of chemotherapy resistance depending on microenvironment, which might be suitable for pharmacological therapies. Comparison of the transcriptional profile of GFP+-sorted E2A-PBX1/preBCR+ leukemia cells in different tissues (bone marrow, spinal cord, lymph nodes, spleen) and after treatment with conventional chemotherapies prednisolone, daunorubicin compared to control (DMSO). Overall design: scRNA-seq samples were generated from 5 tissues (bone marrow, spinal cord, brain, lymph nodes, spleen) from 3 mice (15 samples) developing E2A-PBX1/preBCR+ leukemia after treatment with conventional chemotherapies prednisolone, daunorubicin compared to control (DMSO) using the 10X Genomics Chromium platform and protocols.