Transcriptomic profile of valvular endothelial cells in normal and high glucose conditions, interacted with human monocytes (THP-1)
Human valvular endothelial cells were co-cultured with THP-1 monocytes in high glucose (33mM) or normal glucose (5mM) levels for 2 hours. Afterwards cells were lysed and total cellular RNA was extracted using TRIzol reagent.