RNA sequencing analysis of wild-type and Scd1-deficient CD4+ early iTregs
Analysis of differentially expressed genes in CD4+ early iTregs isolated from wild-type and Scd1-deficient mice. The goal of this study was to elucidate the effect of Scd1 on Treg differentation. Overall design: CD4+ T cells were purified from splenocytes (collected from 11-week-old wild-type and Scd1-deficient C57BL/6 mice) using magnetic separation. Subsequent fluorescence-activated cell sorted naïve T cells (CD4+ CD44med CD25- CD62L+) were differentiated for 1 day in Treg-polarizing conditions (CD3, CD28, TGFb). After 1 day, cells were lysed using QIAzol.