Single-Cell RNA-Seq of allogeneic and syngeneic heart grafts in mouse cardiac transplantation
we present the first comprehensive analysis of the heterogeneity in gene expression and cell types in the allogeneic and syngeneic mouse heart grafts using scRNA-seq. We identify 21 distinct cell populations by performing dimensionality reduction and unsupervised cell clustering analysis in the allogeneic and syngeneic heart grafts. Overall design: Single-cell transcriptional profiling using the 10x Chromium platform was performed on two allogeneic heart grafts and two syngeneic heart grafts.