RNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
Bulk RNA-sequencing was performed on E. coli BL21 (DE3) evolved at 25°C in pH 9 terrific broth media buffered with Tris-HCl (pH 9). The evolved E. coli was characterised and compared to the parent strain during protein expression; the strains were actively grown and compared for gene expression at pH 7 and pH 9 in terrific broth media.