Single-cell Structure Probing of RNA Transcripts (sc-SPORT) of hNEU cells
Here, we developed a new method- Single Cell Structure Probing Of RNA Transcripts scSPORT to simultaneously determine RNA secondary structural signal and gene expression in a single cell. We applied it to study RNA structures in hESCs and different stages of neuronal differentiation such as neuronal precursor cells (NPC, seven days post differentiation), immature neurons (iNeu, eight days post differentiation) and early neurons (NEU).