Single-cell transcriptomic mapping of immune cells in mouse bladders during urinary tract infection
To explore the classification and functional roles of bladder immune cells during urinary tract infection (UTI), we performed scRNA-seq analysis of immune cells extracted from mouse bladders. Overall design: Mice were intravesically infected with uropathogenic Escherichia coli(UPEC) CFT073, and bladder immune cells were isolated using flow cytometry 8 hours post-infection.