Homo sapiens Raw sequence reads
We modified a long-range amplicon sequencing method with high accuracy to determine CRISPR-induced large deletions by developing a novel k-mer sequence alignment algorithm. We observed that the large deletions were mostly accompanied with small indels in all human cells we tested, which included not only cancer-like cells including HeLa, HEK293T, U2-OS, K562 but also fibroblasts and primary T cells, and human embryo stem cells (H9). Also, we observed the large deletion in base editors and prime editors and investigated their repair mechanism that generates large deletions.