Blocking Primer Validation for 12S rRNA in Sea Lamprey (Petromyzon marinus)
This study focused on designing and validating the effectiveness of a blocking primer for the 12S rRNA gene in sea lamprey. In addition to gel electrophoresis and quantitative PCR, DNA metabarcoding was used to compare sequence read numbers in samples with and without blocking primers. These samples include single-species compositions and mixed-species compositions, along with control samples of no DNA.