Bulk RNA-seq transcriptome profiling of dorsomedial and ventrolateral human POMC neurons isolated by IHC/LCM-seq
The aim of this study was to characterize the transcriptional differences between the POMC neurons located in dorsomedial and ventrolateral part of the human infundibular nucleus. To achieve this, we adapted our immunoLCM-seq method for application to post-mortem human brain tissue. The method combines immunohistochemical labeling of target neurons with newly developed RNA-preserving conditions to maintain RNA integrity throughout the workflow. Each transcriptomic profile comprised 14,000 - 19,000 expressed transcripts. This approach enabled the direct comparison of dorsomedial and ventrolateral POMC neurons and revealed subregion-specific gene expression patterns in key regulators of energy homeostasis.