Deep protease profiling of activated neutrophil protease signatures
This project involves high throughput sequencing of 5-mer phage display libraries to profile the substrate specificities of neutrophil serine proteases cathepsin G, elastase, and human proteinase 3, as well as the proteolytic activity in the supernatant of activated neutrophils. Sequencing library was constructed as previously described: Kretz, C.A., et al., Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. Proc Natl Acad Sci U S A, 2015. 112(30): p. 9328-33