Identification of RNA molecules bound on IIBs of polyQ-expanded ATXN1
SH-SY5Y cells overexpressing EYFP-ATXN1(Q82) under the control of the Tet-On promoter were generated using the Sleeping Beauty Transposon. These cells accumulate insoluble IIBs of polyQ-expanded ATXN1. In order to determine the identity of the RNA molecules bound on insoluble polyQ IIBs, RNA was isolated fro YFP-ATXN1(Q82) IIBs, followed by RNA-sequencing. RNA was also isolated and sequenced from control samples (CTRL) which did not contain any inclusions.