Escherichia coli str. K-12 substr. DH10B Raw sequence reads
Transcriptome-wide effects of wild-type Cas13d, high-fidelity Cas13d (hfCas13d), and attenuated Cas13d (atnCas13d) on gene targets encoded on high- versus low-copy plasmids in E. coli. We observed that both wild-type and high-fidelity variants caused substantial off-target transcriptome perturbations. When the target gene was carried on a high-copy plasmid (pMB1* origin), wild-type Cas13d, hfCas13d, and atnCas13d induced pronounced transcriptome-wide disruption. In contrast, for targets on a low-copy plasmid (pSC101 origin), transcriptomic perturbation was markedly reduced for hfCas13d and atnCas13d, whereas wild-type Cas13d remained highly disruptive.