PSC co-culture time-course 15 samples single cell RNA-sequencing
The goal of this experiment is to characterize the differentiation trajectories of pancreatic stellate cells (PSC) exposed to tumour-derived signals over a 6 days time series. Co-cultures of PSC and tumour organoids were seeded in matrigel and grown for 0-6 days in basal media. Mono-cultures of PSC were used as controls. The PSCs were recovered by flow cytometry at successive time points and subjected to single cell RNA-seq profiling via the ddSeq-Surecell system.