Physical interaction with Spo11 mediates the localisation of Mre11 to chromatin in meiosis and promotes its nuclease activity
Meiotic recombination is of central importance for the proper segregation of homologous chromosomes and is initiated by DNA double-strand breaks (DSB) that depend on Spo11 and Mre11. Calibrated chromatin immunoprecipitation (ChIP) for Mre11 shows that Spo11 promotes Mre11 recruitment to chromatin, independent of DSB formation. A C-terminal deletion mutant deficient in Spo11 interaction severely reduces the association of Mre11 with meiotic chromatin. Consistent with the reduction of Mre11 foci in this mutant, it strongly impedes DSB formation, leading to spore death. Overall design: Six ChIP-seq samples and six corresponding whole cell extract (WCE) samples were derived from Saccharomyces cerevisiae (S. cer.) SK1 cells with HA-tagged (or untagged) Mre11 grown in meiotic culture for 5 hrs ("t5") and mixed with Candida glabrata (C. gla., with Scc1-HA6) CBS138 cells for calibration and downstream quantiative comparison. Paired-end (75bp) fastq.gz files are provided, as well as calibrated processed files ("filled dpp" with chromosome, position, and depth information).