RNA sequence of the control and INK4a-overexpressed mouse and naked mole-rat fibroblasts
We lentivirally transduced INK4a or venus into mouse and naked mole-rat (NMR) fibroblasts. INK4a-overexpressed mouse and NMR fibroblasts were cultured for 12 days and they showed several features of cellular senescence, including larger and flat morphology, decrease in the BrdU incorporation, increase in the SA-beta-Gal activity, hypophosphorylation of Rb protein and phosphorylation of AKT protein. The venus-overexpressed cells were harvested at 6 days to keep the numbers of passage equivalent to that of INK4a-overexpressed cells. Annexin V/PI staining showed that cell death was significantly increased only in NMR-fibroblasts but not in mouse-fibroblasts 12 days after the INK4a transduction. The data is deep sequencing of mRNA from three lines of NMR-fibroblasts (C42, H45-2 and D2) and two lines of mouse-fibroblasts (6w8 and 6w9). Gene expression profiles of these cell lines were analyzed.