The PAF complex regulates alternative splicing.
The conserved Eukaryotic PAF complex binds to transcribing RNA polymerase II to control deposition of histone marks during transcription. Recently its role in alternative polyadenylation (selection of mRNA 3'end cleavage sites by CPSF) was described. In this work we show that the PAF complex also regulates alternative splicing. Overall design: Effect of depletion of PAF complex subunit [CTR9 (main scaffold) or WDR61] on differential gene expression in HEK293Flip-In T-Rex cell lines.