Bulk RNA transcriptomic analysis of logic-gated CAR-T cells targeting a combination of CD33 and CD123 in AML
We identified a gene set that is differentially expressed in gated versus constitutively expressed CAR-T cells, often pointing to naive and unstimulated genes in gated CAR-T cells, in contrast to constitutive CAR-T cells Overall design: We performed bulk RNA analysis on T cells isolated from CAR-T co-culture with AML  MOLM13 cell line at 48 hours from 3 different donors