Transcriptional landscape changes after tolerization adaptive laboratory evolution of Staphylococcus aureus to naficillin
Two vancomycin-tolerized strains were selected for transcriptional analysis. SNFR strains were tolerized to naficillin in RPMI+10%LB and SNFM were tolerized in Cation Adjusted Mueller Hinton Broth (CAMHB). Overall design: Strains were grown in RPMI or CA-MHB and harvested at OD 0.2.