RNA-seq of ADAR1p110 overexpression in HeLa and HEK293 cells
RNA sequencing data generated to investigate the effects of ADAR1p110 expression on transcriptome regulation and alternative splicing. HeLa and HEK293 Flp-In TREx cell lines were engineered to inducibly express ADAR1p110-GFP and compared with GFP-induced control cells.HEK2393 or HeLa tet-on cells were cultured in DMEM-H supplemented with 10% FBS and 1X Penicillin/streptomycin during 48h to further induce them during 24h (doxycycline (1 ug/ml) in full media) before extraction. Media were removed and cells were washed two times in PBS, to further extract total RNA using Qiagen Rneasy columns (including DNAse treatment). Illumina TruSeq Stranded Total RNA Kit was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.Libraries were subjected to high-throughput RNA sequencing using Illumina HiSeq 4000. The dataset was generated to characterize transcriptomic changes associated with ADAR1p110 expression, including potential alterations in exon usage, alternative splicing, and RNA editing across different cellular contexts.