Altered skin microbiome of patients with filarial lymphedema-Insights from 16S  rRNA gene-based metagenomic studies
16S rRNA gene amplicon-based metagenomic technique used to profile the skin microbiome of patients with filarial lymphedema. Skin swabs taken from the legs of patients with filarial lymphedema and healthy individuals were subjected to Direct Sequencing. DNA was extracted from the skin swab samples using the PowerSoil kit, and the 16S rRNA V3-V4 region was amplified. High-throughput sequencing was performed on the Illumina NovaSeq 6000 platform and bioinformatic analysis was carried out.