High-quality PacBio HiFi genome assemblies for two Triticum aestivum introgression lines (RWG34, RWG51) carrying Thinopyrum ponticum Sr43 segments, plus PacBio Iso-Seq BAMs from RWG34 used for transcriptome-guided annotation.  PacBio HiFi assemblies (RWG34, RWG51) and full-length Iso-Seq reads (RWG34) supporting annotation and analysis of Sr43-associated introgressed regions in wheat.
We provide PacBio HiFi assemblies for two wheat introgression lines, RWG34 and RWG51, which contain Thinopyrum ponticum Sr43-derived chromatin, together with PacBio Iso-Seq full-length transcript data from RWG34. These data supported high-quality structural annotation, isoform discovery, and detailed analysis of introgression breakpoints.