Characterization of shear stress response of in vitro cultured human dermal lymphatic endothelial cells [RNA-Seq]
A hallmark of the endothelial cell phenotype is the response to shear stress. Lymphatic endothelial cells (LECs) lining lymphatic vessels (LVs) have been shown to respond to shear stress induced by the flow of lymph within LVs. Further, it has been shown that shear stress is important for the identity and specialization of the lymphatic vasculature, by inducing the expression of transcription factors and of several other molecules on LECs. Our aim was to build upon this current knowledge, and identify shear stress regulated genes in skin-derived LECs derived. We exposed LECs derived from the foreskin of 3 different human juvenile donors (commercially available) to laminar shear stress, oscillatory shear stress or static conditions. We identified several genes regulated by shear stress. Overall design: To investigate the impact of shear stress on the gene expression of human dermal LECs, we subjected 3 donors of human dermal LECs to in vitro laminar shear stress (4 dyn/cm2), oscillatory shear stress (0.25 Hz, 4 dyn/cm2) or static condition for 48 hours. We then performed bulk RNA-seq on the samples and compared the impact of laminar or oscillatory shear stress to static conditions.