Genome-wide expression analysis of eBL responses in wild type and gtl1df1 Arabidopsis plants
Purpose: The goal of this study is to demonstrate the global expression profile of Arabidopsis wild type and gtl1df1 mutant plants in response to eBL treatment.Methods: Four-week-old Arabidopsis plant leaves were sprayed with sterile water (Mock) or eBL and harvested at 2h after infiltration for RNA extraction and deep sequencing by Illumina. Raw data were cleaned up and trimmed and reads were mapped to Arabidopsis genome. Gene expression levels were calculated using the TPM method (Transcripts per Kb of exon model per Million mapped reads).Results and Conclusions: Many BR-related genes are differentially regulated in the gtl1df1 mutant plants in response to eBL treatment compared with wild type plant.