16S metabarcoding sequencing of surface sediments for Douarmor task (1.4) of BIOcean5D project, 2025
Seasonal variability of microorganism biodiversity in superficial (0-5 cm) sediment across an anthropogenic gradient in the Bay of Naples (Italy) and in the Bay of Morlaix (Fance) over two years. Samples of surface sediment (1-5 cm) were taken on 3 transects (P1, P2, P3), 3 points per transect, once per season over two years (2023-2025), in the Bay of Morlaix (France) and the Bay of Naples (Italy). In the Bay of Morlaix some sediments have been collected in a tidal ecosystem during low tide, and are called intertidal, while the others are called shallow. In Naples sediment has been collected in non-tidal ecosystem as far as ca. 200 m below the sea surface, and are called shallow. Samples in the Bay of Morlaix were taken by Ifremer and the Roscoff Marine Station, and samples in Naples were taken by the Stazione Zoologica Anton Dohrn (SZN) Naples. DNA extractions in Naples were carried out by the University of Naples and for Penze by the Ifremer DYNECO/Pelagos laboratory. Amplification of 16S rRNA V4-V5 regions (Parada 2016) was carried out by Ifremer DYNECO/Pelagos laboratory.