Bulk RNA-seq of primary keratinocytes treated with IgG from patients with bullous pemphigoid or controls II
While antibodies in bullous pemphigoid (BP) are known to activate innate immune response, their direct effect on keratinocytes and the overall contribution of keratinocytes to disease pathogenesis is largely unknown. We sought to understand the mechanism of keratinocyte response to autoantibodies in BP.  We affinity purified IgG from patients with a known diagnosis of bullous pemphigoid or healthy controls, and performed bulk RNA-seq. We identified numerous differentially expressed genes including upregulation of CXCL16, IL-24, and TGFB1 in BP-IgG treated keratinocytes Overall design: Primary human keratinocytes were treated with 4mg/mL of affinity purified IgG from patients with bullous pemphigoid (n=4) or control (n=4)