ATAC-seq of primary mammalian aortic endothelial cells before and after TNFa stimulation
To identify conserved TNFa-induced changes in chromatin-accessibility in mammals, we performed ATAC-seq in primary vascular endothelial cells (ECs) isolated from the aortas of human (HAEC), mouse (MAEC) and cow (BAEC), before and after TNFa. We overlay our data with multi-species NF-?B binding data and identify multiple modes of NF-?B-chromatin interactions that are conserved during mammalian TNFa response. Our cross-species approach identifies conserved changes in chromatin-accessibility at NF-?B binding sites that are disease-relevant and essential during mammalian acute inflammation.