Transcriptional profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 heterozygous and homozygous mice [Msh3_striatum]
As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington's disease (HD) through various computational modeling efforts. Striatum was isolated from 12-month-old Msh3+/- mice and Msh3-/- (KO) crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, 8 replicates per genotype. Overall design: RNASeq analysis was performed on samples from the striatum tissues of 12-month-old Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, and wild-type mice. N = 8 per group (4F/4M).