Illumina amplicon sequencing of three different soil denitrifying communities
In this study we aimed to determine denitrifier and DNRA community structures in contrasting German soils by 16S rRNA and functional gene (narG, napA, nirK, nirS, nosZI, nosZII and nrfA) amplicon sequencing, quantify those gene via qPCR, assess apparent Michaelis-Menten kinetics for denitrification and N2O reduction and determine key taxa and indicator genes for denitrification and N2O reduction. Relating microbial community composition to reaction kinetics can help to reveal important ecological drivers of microbial communities and their activities, and will provide valuable data for denitrification models.