Carbohydrate-digesting enzyme supplementation shifts ruminal microbiota but affects ruminal digestibility and fermentation temporarily in in vitro fermentation system
This study aimed to evaluate the effects of exogenous enzyme supplementation at specific time points (1.5 h, 3 h, 6 h, 12 h, and 24 h) in a ruminant in vitro experiment. Samples were amplified and sequenced using the Illumina MiSeq short-read sequencing platform (Illumina, Inc., CA, USA) with 16S rRNA V3-V4 primer pairs 341F and 805R .