C2C12 single cell transcriptome during differentiation and K-Ras modulation
Samples were prepared using Chromium Flex Gene Expression assay to capture normal C2C12 cell proliferation (day 0, high serum) and differentiation (day 3 in low serum), respectively. For the latter condition the impact of the knockdown of K-Ras4B, transformation by K-RasG12C or knockdown of ciliary protein Ift88 on differentiation was further assessed.