Gene expression profile of either Dead neuron+/Fcrls+/CD11b+/Ly6C- or Dead neuron-/Fcrls+/CD11b+/Ly6C- microglia from the brain
We used bulk RNA sequencing to investigate the molecular signature of both phagocytic microglia and non-phagocytic microglia affected by deletion of Itgb8 in microglia. Overall design: Dead neuron+/Fcrls+/CD11b+/Ly6C-  and Dead neuron-/Fcrls+/CD11b+/Ly6C- microglia were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using Smartseq2 RNAseq.