Transcriptional profiles of Tead1/4 Knockout and Unedited CT26 Cells
The Hippo kinase cascade converges on the transcriptional effectors YAP (Yes-associated protein) and TAZ (transcriptional co-activator with PDZ-binding motif), which regulate gene expression programs by interacting with the transcriptional enhanced associate domain (TEAD) family of transcription factors (TFs). As a key pathway in organ growth, tissue homeostasis, and regeneration, the YAP/TAZ-TEAD complex controls the transcription of genes crucial for replication, S-phase entry, and mitosis. To further elucidate the transcriptional regulatory network, we monitored the target genes of TEAD1 and TEAD4 using CUT&Tag and transcriptome sequencing. Overall design: The samples in this study included normal CT26 unedited group and Tead1/4 knockout group. Each group contained four samples.