AAV capsid prioritization in machine-perfused normal and steatotic human livers
Adeno-associated virus (AAV) vectors generated with five different capsids were intravenously injected into human livers undergoing normothermic machine perfusion (NMP). To assess the tropism of the five AAV vectors in the human livers, single-cell suspensions of hepatocytes and non-parenchymal cells (NPCs) were analyzed by single-cell RNA sequencing (scRNAseq). Differentially expressed genes were identified in AAV vector-transduced hepatocytes and unique cell populations. Overall design: Hepatocytes and NPCs were released from human livers by enzymatic perfusion or chunk digestion and analyzed using scRNAseq (SeqWell).