Zebrafish Forebrain Methylome
The zebrafish forebrain is a central hub for cognitive and social behaviors, yet a comprehensive, region-specific DNA methylation reference for this tissue is not available. To address this gap, we generated a genome-wide methylation map of the adult zebrafish (Danio rerio) forebrain using Oxford Nanopore Technologies (ONT) long-read sequencing. Forebrains from six biological replicates (four males and two females) were profiled, enabling direct detection of multiple DNA base modifications at single-base resolution. The resulting dataset includes CpG-associated 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), as well as non-CpG 5mC and N6-methyladenine (6mA), achieving 96.8% genome coverage. CpG methylation was widespread, with 64.2% of CpG sites classified as highly 5mC-modified. In contrast, 5hmC and non-CpG methylation were markedly less abundant, and 6mA was detected at very low levels. CpG islands exhibited a bimodal methylation distribution, promoter regions showed elevated variability compared to gene bodies, and CC-context non-CpG methylation displayed significant strand asymmetry. This resource provides a high-resolution epigenomic baseline for the adult zebrafish forebrain.