Genome-wide EGR1 chromatin occupancy in wild-type C57BL/6J bone-marrow–derived macrophages measured by CUT-taq
This study generated CUT-taq (tagmentation-based in situ ChIP; CUT&Tag-like) sequencing data to profile genome-wide EGR1 chromatin binding in mouse bone-marrow–derived macrophages (BMDMs). BMDMs were differentiated from wild-type C57BL/6J bone marrow in GM-CSF for 7 days, harvested at 4.0 × 10^5 cells per sample, and processed using the Hyperactive™ In-Situ ChIP Library Prep Kit for Illumina (Vazyme TD901–TD902). Cells were immobilized on concanavalin A beads, permeabilized with digitonin, incubated with anti-EGR1 primary and secondary antibodies (Thermo), and tagmented with Hyperactive pA-Tn5 (Vazyme). Libraries were PCR-amplified with P5/P7 primers, quality-checked on an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 (paired-end 150 bp). Raw sequencing files (FASTQ) and processed data files are provided to enable reuse and reanalysis of EGR1 binding profiles in GM-CSF–differentiated macrophages. Overall design: Genome-wide EGR1 binding was profiled by CUT-taq in GM-CSF–differentiated wild-type C57BL/6J BMDMs. Biological replicate samples were prepared from independent macrophage preparations, and each replicate was processed through the complete CUT-taq workflow (concanavalin A bead binding, digitonin permeabilization, anti-EGR1 antibody binding, pA-Tn5 tagmentation, PCR amplification, and Illumina sequencing). Sequencing was performed on Illumina NovaSeq 6000 with paired-end 150 bp reads. The submission includes raw FASTQ files (and associated metadata) and, where applicable, processed outputs (e.g., aligned files and peak calls) to support reproducible downstream analyses of EGR1 chromatin occupancy.