Nascent-seq of HeLa WT and SRSF5 KO cells
We used HeLa cells where SRSF5 was knocked out by CRISPR/Cas9 to perform Nascent-seq. WT cells served as control. One hour before harvesting, cells were incubated with 4-sU for exactly 1 hour to label newly transcribed RNAs. 4-sU-labelled RNAs were biotinylated and purified over streptavidin beads and transformed into an RNA-seq library. We compared differential expression and intron retention between WTand SRSF5 KO samples. Overall design: We sequenced 2 biological replicates for all conditions (WT and KO) with 150-bp paired end reads and 100 Million reads per replicate.