GmNMHC5, A Neoteric Positive Transcription Factor of Flowering and Maturity in Soybean
Soybean (Glycine max (L.) Merr.) is an important oil and food crop. Its growth and development is regulated by complex genetic networks, and there are still many genes with unknown functions in the regulation pathway. In this project, GmNMHC5, a member of the MADS-box protein family, was found to promote flowering and maturity in soybean. Gene expression profiling in transgenic plants confirmed that the 35S:GmNMHC5 T3 generation had early flowering and precocity. To further investigate the molecular mechanism of GmNMHC5, we used transcriptome sequencing (RNA-Seq) to explore differential gene expression in response to over-expression of GmNMHC5. For the transcriptome analysis, two biological replicates of transgenic mutants were analyzed. We used wild-type Jack as the control group, and 35S:GmNMHC5#25 and 35S:GmNMHC5-#32 as the experimental groups. The materials were grown in a controlled culture room at 28 degree under LD conditions, and leaf samples were taken at 31 DAE. Each sample consisted of material collected from three individual plants.