QuantSeq of HFFF-TERTs infected with Sendai Virus
QuantSeq of immortalised primary human foetal foreskin fibroblasts (HFFF-TERTs) infected with Sendai Virus (SeV) or mock infected. Experiments were performed in two rounds. In the first round cells were depleted of both CRTC2 and CRTC3 using siRNAs, or treated with a control siRNA. In the second round of experiments HFFF-TERT cells had either CRTC2, CRTC3 or both CRTC2&CRTC3 knocked out using a CRISPR-Cas9 system. Knock-out cells were complemented with either full length CRTC2, full length CRTC3, a version of CRTC2 lacking the N-terminal 50 amino acids (??50), a version of CRTC3 lacking the N-terminal 50 amino acids (??50), both full length CRTC2 and CRTC3, or a vector only control.