Expression profiles of BMMs and osteoclasts
To profile the overall transcriptomic changes, we performed RNA-seq with bone marrow macrophages (BMM) before and after osteoclast differentiation (day 0 vs day 4). To induce mature osteoclasts formation, BMM were seeded and culture with customized aMEM containing 1/50 CMG14-12 supernatant and 75 ng/ml RANKL for 4 days. Overall design: Comparative gene expression analysis of RNA seq data for BMM (day0) and mature osteoclast (day4)