IvtmRNA and SGK1
SGK1 plasmids were commercially purchased from MRC PPU Dundee, UK and have been previously characterised for their function. SGK1 and its variants were subcloned into the pVAX.A120 vector containing, a poly(A)-120 tail using sticky-end ligation. The clones were then subject to sanger sequencing to confirm the assmembly and their mutational status.