Gene expression profile of Dead neuron+/Fcrls+/CD11b+/Ly6C- microglia from the brain
We used bulk RNA sequencing to investigate the molecular signature of phagocytic microglia affected by deletion of Spi1 in microglia. Overall design: Dead neuron+/Fcrls+/CD11b+/Ly6C- microglia were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using Smartseq2 RNAseq.