AFR1 deletion strain derived uniconazole adaptors
Gene AFR1 was deleted from Cryptococcus neoformans lab strain H99. Approximately 1 million cells of the deletion strain were spread on YPD-agar plate supplemented with 0.125ug/mL uniconazole. Randomly 30 adaptors were chosen. 16 adaptors were resistant to uniconazole and were sequenced.