Fusion oncoproteins and cooperating mutations define disease phenotypes in NUP98-rearranged leukemia [CUT&RUN]
This series CUT&RUN data aims to investigate epigenetic profiling of primary leukemia samples, cell lines, or cord blood CD34+ cells transduced with various NUP98 fusion oncoproteins. Overall design: NUP98 fusion oncoproteins binding was assessed by CUT&RUN using HA antibodies in cord blood CD34+ cell models with HA-tagged NUP98 fusion oncoproteins or N-terminus NUP98 antibody in primary samples and cell lines. Epigenetic status and binding of epigenetic regulators (KMT2A, menin) were also assessed. Peaks are called using MACS2 for narrow peaks and SICER for broad peaks. Differential epigenetic status was assessed using PBS (probability-being signals). *************************************************************** Raw files for human/patient samples were not submitted to GEO due to concerns about submitting personally identifiable sequence data for open access. ***************************************************************