acclimated sludge for high-oil Raw sequence reads
Sludge was acclimated to accommodate the high-oil condition. Quantitative edible blended oil was fed into the sludge continuously for 30 days, leading to the oil contents of the sludge reaching 10% (wet basis) on day 30. Mesophilic anaerobic digestion requires that the anaerobic incubator be kept at a constant temperature of 35 .