The first complete circular genome of Paenimyroides ceti strain MC-ZM-24, isolated from Zophobas morio larvae in Switzerland. The genome provides insights into the intrinsic metallo-ß-lactamase repertoire and antimicrobial resistance determinants within the Flavobacteriaceae family.
Paenimyroides ceti is a recently defined member of the family Flavobacteriaceae, originally isolated from marine mammals. Here we report the first complete circular genome of P. ceti strain MC-ZM-24, which was isolated from homogenized tissues of Zophobas morio larvae in Switzerland. Whole-genome sequencing was performed using both Illumina NovaSeq 6000 (short reads) and Oxford Nanopore MinION Mk1B (long reads), followed by hybrid assembly with Unicycler and annotation using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The resulting genome consists of a single circular chromosome of 3,274,043 bp (GC content 35.56%), encoding 3,053 genes. The strain exhibited multidrug resistance, including reduced susceptibility to carbapenems, and harbored a unique chromosomal metallo-ß-lactamase (MBL) gene distinct from previously characterized subclass B1 enzymes. This study expands genomic knowledge of Paenimyroides species and highlights the evolutionary diversity of chromosomal MBLs within the Flavobacteriaceae.