RNA-seq of mouse ME3 control and Irx3-KO cells on days -1, 1 and 9 of adipogenic differentiation after treatment with DMSO or the SUMOylation inhibitor ML-792
ME3 control and Irx3-KO mouse beige-like preadipocytes were grown in proliferation medium until confluence (day -2), before induction of adipogenesis on day 0 followed by adipogenic differentiation for 9 days. Cells were treated with either DMSO or 0.5 uM ML-792 for 24h either from day -2 to day -1 or day 0 to day 1, or throughout the differentiation from day 0 to 9 (n =3 replicate wells for each condition) before collection by trypsination, centrifugation and snap-freezing in liquid N2 on days -1, 1 and 9, respectively. Cell pellets were kept at -80C until RNA isolation.  RNA was isolated using the RNeasy protocol with on-column DNase treatment.  *** insert info about RNA QC, library prep flow cell, bioinformatics etc