Bulk K562 and EL4 cells mixed with 12 Time-Tags for verification (QC) for ChronoSeq Beads
We checked our ability to uniquely identify each Time-Tag. We did this by mixing beads from odd numbered Time-Tags with human cells and even numbered Time-Tags with mouse cells manually in bulk. These beads were then washed and combined for Reverse Transcription, library preparation and sequencing. Time-Tag resolved cleanly into either human or mouse dominant with 1.6% or less mixed beads detected for each Time-Tag.