Idnetification of a new HLA-DRB1*11:03 in a french  voluntary bone marrow donor
Here we describe a novel DRB1*11 :03 allele, identified in  a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following: A*24:02 A*34:02 B*08:01 B*37:01 C*06:02 C*07:01 DRB1*03:01 DRB1*11:03 DRB3*01:01 DRB3*02:02 DQA1*05:01 DQA1*05:05 DQB1*02:01 DQB1*03:01/03:276N DPA1*01:03 DPA1*02:01 DPB1*01:01 DPB1*04:02  HLA-DRB1*11 :03 : New allele differs from DRB1*11 :03 by one nucleotide substitution at position 34 of exon 3 (position 406 of cDNA), where  base C is replaced by T  inducing Ile instead Thr. In fact, the  106th codon of HLA-DRB1*11:03 :01 (ACC coding for Thr ) becomes ATC  coding for Ile in this New DRB1*11 :03.