Single nuclei RNAseq of skin sensory neurons during psoriasis
The peripheral nervous system plays important roles in orchestrating tissue function in health and disease. In this work, we perform single nuclei RNAseq of skin sensory neuron DRGs in a mouse model of psoriasis to interrogate the impact of skin inflammation on the function and transcriptome of skin sensory neurons. Overall design: 10000 nuclei per lane isolated from dorsal root ganglion of mice (total of 6 lanes, 3 Control and 3 S. aureus IMQ) were loaded on a Chromium Single Cell Controller (10X Genomics) to encapsulate the nuclei into droplets. The gene expression (GEX) library was prepared using Chromium Single Cell 5' v2 reagent kits following the manufacturer's instructions, and sequenced on an Illumina Nextseq2000 platform