Single-cell RNA-sequencing reveals the role of p300/CBP in calcium handing ability of prepubertal RV cardiomyocytes.
The expression of genes is determined by the openness and accessibility of chromatin, which is the most basic phenomenon in the field of life sciences.The histone acetylase p300/CBP (CBP and P300 have similar structures and functions) can catalyze the acetylation of the lysine at the 27th position of histone H3(H3K27ac), which changes the the openness and accessibility of chromatin. Thus, p300/CBP are considered to be two of the most important transcription co-factors. To further understanding the role of p300/CBP in regulating calcium handing of prepubertal RV CMs, we first detected its expression in the prepubertal RV.The expressions of p300/CBP in the prepubertal dysfunctional RV were significantly downregulated, consistent with the data of p300-ChIP-seq, which showing the loss of p300 chromatin occupancy in calcium handing genes. Then we knocked out p300/CBP in neonatal mouse. p300/CBP knockout cardiomyocytes showed a impaired calcium handing ability, similar to that in prepubertal dysfunctional RV cardiomyocytes. These results confirmed the role of p300 in regulating the calcium handing ability of prepubertal RV CMs. Overall design: We used C57/BL6J mice in this study and knocked out p300/CBP in neonatal mouse. The right ventricular tissue of mouse pups on postnatal day 21 were collected for ScRNA-seq analysis.