snRNA-seq of nuclei isolated with microvessel-enrichment procedure applied to frozen post-mortem brain tissue
Vascular cell types are under-represented in standard single-nucleus RNA-Seq studies of human frozen post-mortem brain tissue. The dataset represents the pilot data obtained with the first version of a microvessel- enrichment protocol to obtain a higher percentage of endothelial cells as the key cellular component of the blood-brain barrier. Overall design: Two brain samples from one healthy control and one Alzheimer's disease patient (BRAAK stage 6 with amyloid angiopathy), were processed as described in the extract protocol and snRNA-Seq using the 10x Genomics platform was carried out.