Single Cell Sequencing of Mouse Embryo at Early and Mid Gestation
Maternal adaptations are necessary for pregnancy and proper nurturing of a developing embryo. Murine embryogenesis and pregnancy are intensely studied processes, but historically this knowledge has been acquired by analyzing the role of a single molecule at a time. Single cell RNA-sequencing allows for a snapshot of the of cell states for multiple cell populations that can be compared to understand time-dependent pregnancy adaptations, and the dynamic cellular composition of murine embryogenesis during gestation. Here we present scRNA-seq analyses on murine implantation sites at gestational day (GD) 6.5 and 10.5 and examine the temporal changes that occur from early to mid-gestation. These analyses were conducted on C57/bl6J mice to serve as a resource to the community. We have also made an interactive webpage with these datasets, providing way for anyone to further explore genes and cell types of interest without the need for coding skills. The interactive datasets can be found at (link) Overall design: Uteri from pregnant dams were dissected at GD 6.5 and 10.5 and the gestational sacs were separated. For GD 10.5, the fetus and the placenta were removed. For the GD 6.5 samples, two different gestational sac from two different mice were used. For GD 10.5, two different gestational sac from one dam were used, and the tissues were analyzed using scRNA-seq