The effect of an NAT10 inhibitor (remodelin) on inflammation-related genes in the treatment of thalamic hemorrhage based on RNA sequencing data
Thalamic hemorrhage is a disease caused by hemorrhage of the thalamic brain parenchyma and is accompanied by an inflammatory response during development. NAT10 has been associated with a wide range of diseases. The aim of this study was to explore the molecular mechanisms associated with the use of the NAT10 inhibitor (Remodelin) for the treatment of thalamic hemorrhage from an inflammatory perspective. Overall design: First, the total RNA of the sample was isolated and purified by TRIzol (Invitrogen, CA, USA), which was used for quality control by a NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The integrity of the RNA was subsequently detected with a Bioanalyzer 2100 (Agilent, CA, USA) and verified via agarose gel electrophoresis. RNA samples with a concentration >50 ng/µL, a RIN >7.0, an OD260/280>1.8, and >1 µg of total RNA were selected for subsequent experiments. Then, the mRNA captured by oligo(dT) magnetic beads was fragmented at high temperature and reverse transcribed into cDNA. Next, the second strand of cDNA was synthesized by RNase H digestion, and the ends of the double-stranded DNA were aligned to form flat ends. Then, an A base was added at each end of the double-stranded DNA so that it could be ligated to the junction with a T base at the end of the double-stranded DNA. Finally, magnetic beads were used for the selection of fragment sizes and purification so that fragments could be formed into 300 bp±50 bp fragments