IBD inception cohort microbiota
The gut microbiota contributes to the etiopathogenesis of inflammatory bowel disease (IBD), but limitations of prior studies include the use of sequencing alone (restricting exploration of the contribution of microbiota functionality) and the recruitment of patients with well-established disease (introducing potential confounders, such as immunomodulatory medication).  Here, we analyze a true IBD inception cohort and matched healthy controls (HCs) via stool 16S rRNA gene sequencing and multi-system metabolomic phenotyping (using nuclear magnetic spectroscopy and mass spectroscopy), with subsequent integrative network analysis employed to delineate novel microbiota-metabolome interactions in IBD.  Marked differences in ß diversity and taxonomic profiles were observed both between IBD and HCs, as well as between Crohn's disease (CD) and ulcerative colitis (UC) patients.  Multiple between-group metabolomic differences were also observed, particularly related to tryptophan-/indole-related metabolites; for example, UC patients had higher levels of serum metabolites including xanthurenic acid (q=0.0092) and picolinic acid (q=0.018).  Network analysis demonstrated multiple unique interactions in CD compared to HCs with minimal overlap, indicating a loss of 'health-associated' interactions in CD.  Compared to HCs, UC patients demonstrated increased pathway activity related to nitrogen and butanoate metabolism, whilst CD patients displayed increased leucine and valine synthesis.  Networks from IBD patients overall showed negative correlation with health-specific associations, including an increase in taurine metabolism.  Collectively, this work characterizes multiple novel perturbed microbiota-metabolome interactions that are present even at the diagnosis of IBD, which may inform potential future targets to aid diagnosis and direct therapeutic options.