MethylRAD-seq of Crassostrea virginica; whole body
The goal is to assess the influence of early life environments on DNA methylation in juvenile eastern oysters in a later environment. In a fully-factorial design, oysters were exposed in two phases to water with manipulated dissolved oxygen (normoxic or hypoxic, <2 mg/L). Whole-body tissue samples were sampled for methylRAD-sequencing to identify patterns of DNA methylation.