RNA-seq analysis of renal organoids
Objective: To investigate the direct protective effect of polymyxin B on lipopolysaccharide (LPS)-induced sepsis-associated acute kidney injury and clarify the regulatory mechanism of protein SUMOylation in this process. Methods: Renal tissues were extracted from C57BL/6 wild-type (WT) and kidney-specific SUMO1-overexpressing (SUMO-KO) mice to construct kidney organoid models. The organoids were divided into three groups: the control group treated with 0.2% DMSO for 24 h, the LPS group (treated with 10 ug/ml LPS + 0.2% DMSO for 24 h), and the PMB group (pretreated with 10 ug/ml LPS for 6 h, followed by treatment with medium containing 5 ug/ml PMB for 18 h). Transcriptomic analysis was performed using RNA-seq. A total of 14 cases were included: 4 cases each in the WT-LPS and WT-PMB groups, and 3 cases each in the SUMO-LPS and SUMO-PMB groups.