Transcriptome change after miR-206 overexpression
We overexpressed microRNA-206 in heptocytes to evaluate its effect on transcriptome. Overall design: Eight-week old male C57BL/6J mice (Jackson Laboratory, n=3) were hydrodynamically injected with 20 µg pT3-TTR-miR-206-IRES-GFP (TTR promoter drive miR-206 and GFP transcription) and 0.8 µg pCMV/Sleeping Beauty Transposon (SB). Four weeks post injection, primary hepatocytes were isolated via perfusion as previously described.(3) Isolated hepatocytes were subjected to flow cytometry sorting, both of GFP negative (control) and positive hepatocytes (miR-206 overexpressed) were collected for total RNA extraction (Qiagen RNeasy Micro Kit). The library was prepared and sequenced via ultra-low input RNA sequencing in GENEWIZ (New Jersey).