Impairment of spermatogenesis and sperm motility after fecal microbiota transplantation from Wip1 knock-out mice with subfertility phenotypes (Metagenome)
In this study, we investigated whether the fecal microbiota transplantation (FMT) obtained from Wip1 KO mice impaired the spermatogenesis functionality in mice treated with an ABX antibiotic cocktail. The sperm quality and metabolic parameters were evaluated and compared with those of wild type (WT) mice. Multi-omic analysis including proteome, metagenome, metabolome and lipidome were performed. FMT further revealed a linkage between gut microbiota and impaired spermatogenesis.