Effect of depletion of Spi1, Cebpb, and Jun on gene expression during therapy-induced-senescence of primary, murine Eµ-myc;bcl2 B-cell lymphomas
Therapy-induced senescence associates with expression of lineage-aberrant transcript profiles. Spi1, Cebpb and Jun encode important hematopoietic transcription factors. To investigate their impact on lineage-adherence in therapy-induced senescence of B-cell lymphomas, we engineered several senescence-susceptible and senescence-deficient individual primary, murine Eµ-myc lymphomas overexpressing Bcl2, in which each target gene has been knocked down by shRNA. We then treated lymphomas with adriamycin (doxorubicin) to induce senescence and performed gene expression analysis using data obtained from RNA-seq of the individual lymphoma samples. Overall design: Examine differentially expressed gene transcripts in senescent and non-senescent conditions between control (sh_Ctrl) and specifically targeted (sh_Spi1, sh_Cebpb, sh_Jun) lymphoma derivatives.