CelFi: A Rapid and Robust Validation Strategy for Pooled CRISPR Screens
A CRISPR-based method for hit validation that tests the effect of a genetic perturbation on cell fitness. By editing target loci and monitoring out-of-frame (loss-of-function) allelic profiles over time, we have created a Cellular Fitness (CelFi) assay that can elucidate cellular vulnerabilities and verify hits from pooled CRISPR screens.