MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer [CUT&RUN]
The effect of gene perturbation on the histone mark H3K27ac was evaluated in human Cas9 expressing MDA-MB231 cancers Overall design: Human pancreatic cancer cell lines were cultured in DMEM supplemented with 10% fetal bovine serum (FBS). CUT&RUN was performed according to manufacturer's instructions (EpiCypher-CUTANA Kit) and Sequencing libraries were generated using  Ultra II DNA Library Prep Kit (NEBNext)