Transcriptomic Validation of ALS-Derived Motor Neurons Following m6A Hypomethylation Restoration
In this experiment, we investigated transcriptomic alterations in iPSC-derived motor neurons from ALS patients following the restoration of m6A hypomethylation to mitigate neurodegenerative processes. Overall design: To explore whether the transcriptomic level rescued upon restoring the m6A level in degenrated ALS iPSC~MNs. We differentiated two fALS patient (C9ORF72exp~800 G4C2 and SOD1+/L144F) iPSC~MN lines and one sALS iPSC~MN line under defined conditions to cause MN degeneration through a selective ER stressor, CPA. Subsequently, we applied FB23-2 to determine if doing so could elevate m6A levels and thereby restore the m6A repertoire to rescue MN degeneration in different contexts of ALS. Finally thtoal RNA were harvest and performed the Illumina NextSeq 500.