Transcriptional profiling of single cells from epithelial organoids modelling Intestinal tumorigenesis
To chart cell state dynamics across the malignancy continuum of colorectal cancer, wild type organoids (WT), ApcKO (A) and ApcKO::KrasG12D::Smad4KO::Trp53KO (AKSP) tumoroids were subjected to single cell RNA-seq. Overall design: Isogenic lines with the indicated genotypes were collected for s.cRNA-seq before reaching confluency to minimize cell death.