Microbial diversity  in alpine wetland soil
For this study, we selected 82 alpine wetlands from 19 regions in the Qinghai-Tibet Plateau.Field sampling was conducted from June to September 2023 during the growing season. The water depth in alpine wetland ponds ranges from 10 to 40cm. Surface soil samples from 0-10 cm depth were carefully excavated using a shovel, removing larger stones, gravel, and live plants before transferring them into polyethylene self-sealing bags. For microbial analyses, soil samples were collected and placed in sterile microcentrifuge tubes.