Effect of MSMP inhibition on the gene expression profiles of rheumatoid fibroblast-like synoviocytes
To explore the downstream target of MSMP, RNA-sequencing (RNA-seq) was used to display gene expression profiles in fibroblast-like synoviocytes (FLSs) from 3 patients with rheumatoid arthritis (RA) between control siRNA (siC) group and siMSMP-2 group (with the highest interference efficiency for MSMP). The heatmap and volcano diagram showed a total of 216 differentially expressed genes with 77 upregulated genes and 139 downregulated genes (Fold change=1.5,p<0.05) Overall design: We demonstrated gene expression profiles between control siRNA (siC) group and MSMP knock down group (siMSMP-2) in FLSs from 3 patients with rheumatoid arthritis (RA) using RNA sequencing (RNA-seq)