Cut&Run analysis for transcription factors in MOLT-4 cells.
Cut&Run analysis was performed in MOLT-4 cells to analyze the basal level of H3K4me3 and DNA binding of RUNX1 and SMARCA4. Overall design: MOLT-4 cells were incubated with CUTANA pA/G-Mnase. Chromtin fragments were purified utilizing a Cutana DNA purification kit. Libraries were generated using a NEBNext Ultra II DNA library prep kit and analyzed on Illumina NovaSeq at GENEWIZ Azenta Life Sciences Co. Ltd.