Complete genome sequence of Clostridium botulinum TMIPH-CB191 (GTC18335) isolated from a human in Japan.
Clostridium botulinum is a Gram-positive, spore-forming, anaerobic bacterium that belongs to the family Clostridiaceae. This particular strain was first isolated from a human in 1996 and was submitted to our laboratory as a historical strain. In this study, the complete genome sequence of the aforementioned strain was determined. Whole-genome sequencing was performed using a combination of long-read sequencing with PromethION 24 (Oxford Nanopore Technologies, Tokyo, Japan) and short-read sequencing with NextSeq 1000 (Illumina, San Diego, CA, USA). The hybrid assembly of short and long reads was conducted using Unicycler v0.4.8 with default parameters. The process of genome circularisation was executed automatically during the assembly procedure. This complete genome sequence is of considerable value for comparative genomic and epidemiological studies.