Mouse model of steatohepatitis
This study aims to investigate the differences in gene expression between normal mice and mice with hepatic steatosis to reveal the molecular mechanisms underlying the development and progression of fatty liver disease and to provide potential targets for the prevention and treatment of related diseases. C57BL/6J mice were selected as the experimental subjects, and a fatty liver inflammation model was established by feeding them a high-fat, high-fructose, and high-cholesterol (FFC) diet. Specifically, the FFC diet consisted of 40 kcal% fat, 20 kcal% fructose, and 2% cholesterol, and the mice were continuously fed this diet for 24 weeks. Afterward, we monitored the total cholesterol (TC) levels in the mice, and the results showed a significant increase in total cholesterol in the model group. By comparing the gene expression profiles of normal mice and mice with fatty liver inflammation, we hope to identify key genes and signaling pathways associated with fatty liver disease, thereby deepening our understanding of the pathophysiological processes of hepatic steatosis and laying the foundation for the development of new diagnostic markers and therapeutic strategies. Overall design: RNA-seq analysis of liver tissues of 6 normal control mice and 18 model mice