Comparison of techniques and markers to distinguish Plasmodium falciparum recrudescence from new infection in Rwanda
This study investigates molecular approaches for PCR correction in therapeutic efficacy studies of Plasmodium falciparum malaria. Amplicon sequencing and fragment analysis were performed on clinical samples collected in Rwanda to genotype polymorphic markers (msp1, msp2, glurp), microsatellites, and SNP-rich loci (ama1, cpmp, cpp, csp). The data were used to compare marker performance and classification algorithms for distinguishing recrudescence from new infections in recurrent malaria cases. This dataset supports the evaluation and development of robust, reproducible methods for estimating antimalarial drug efficacy.