Role of mucin MUC5AC on tumor angiogenesis and lung adenocarcinoma development
MUC5AC is a secretory type of mucin, which is significantly overexpressed in lung adenocarcinoma and associated with poor survival outcomes. Expression of MUC5AC and its sialylation is required for alteration of tumor matrix remodeling through interacting with integrin ß4 in lung adenocarcinoma. We have previously shown that sialylated MUC5AC is required for lung adenocarcinoma cell growth and metastasis. To understand the molecular mechanism of MUC5AC in lung adenocarcinoma development, we performed RNA sequence analysis using MUC5AC knockdown cells (A549-shMUC5AC) and scramble cells (A549-shRNA). We observed that several tumor matrix proteins including VCAN and integrin ß4 were significantly downregulated and are associated with tumor angiogenesis. In this study, we reported that sialylated MUC5AC induces tumor angiogenesis by altering tumor matrix protein VCAN during lung adenocarcinoma development and metastasis. Overall design: Sample details: MUC5AC knockdown lung adenocarcinoma cells-A549-shMUC5AC (N=3) Scramble lung adenocarcinoma cells-A549-shRNA (N=3)