An Illumina-based amplicon sequencing approach designed to determine grapevine fanleaf virus isolates
Description of an Illumina-based amplicon sequencing (AmpSeq) approach, associated with an analytical workflow for rapid diagnostic, combining HTS quality screening at a more affordable cost. Our multi-primers assay was designed to identify a large diversity of both genomic RNAs (RNA1 and RNA2) of Nepovirus foliumflabelli (also known as Grapevine fanleaf virus, GFLV), compatible with the known genetic diversity of this bipartite virus down to detecting rare variants (i.e. present at less than 2% in an isolate).