RNAseq for cassava pathogen Xanthomonas phaseoli pv. manihotis
RNAseq for cassava pathogen Xanthomonas phaseoli pv. manihotis strain CIAT1241: In vitro-propagated cassava cv. 60444 plants were infiltrated with the Xpm strain CIAT1241 (OD600=0.5) or mock-inoculated with 10 mM MgCl2. Infiltrations were performed on the abaxial side of one leaflet per plant, using two biological replicates per treatment. At 50 hours post-inoculation (hpi), infiltrated tissues were harvested, flash-frozen in liquid nitrogen, and ground in a TissueLyser II. Total RNA was extracted using the Invitrap Spin Plant RNA Minikit (STRATEC, Germany) with the RP buffer and treated with TURBO DNase (Thermo Fisher Scientific, USA). Barcoded TruSeq RNA paired-end libraries were pooled and sequenced across four flow cells of the Illumina NextSeq500 system.