Function of the TAp63 variant expressed from TP63 (p63)
To gain insights into the function of poorly expressed TAp63 variant of TP63, TAp63 knockout cells were produced from squamous cell carcinoma line FaDu by CRISPR-Cas9. To assess the off-target effects of CRISPR-Cas9, the parental cell line and a TAp63 knockout cell line were compared using the whole genome sequencing.