Whole genome sequencing data in three strains of mouse models with high-accurate PECC-Seq analysis
To improve the accuracy of NGS in the precise clarification of somatic mutations that only present in a small fraction of cells or even in single cells, we developed a high-accurate PCR-free duplex consensus sequencing approach, the Paired-End and Complementary Consensus Sequencing (PECC-Seq). PECC-Seq employed shear points as endogenous barcodes to identify consensus sequences from the overlap in the shortened, complementary DNA strands-derived paired-end reads for sequencing error correction. We applied PECC-Seq to detect the spontaneous and chemically-induced ultra-low-frequency mutations in three mouse models, i.e., gpt delta transgenic mouse, C57BL/6J mouse and DBA2 mouse.