H3K27me3 CUT&Tag in keloid derived fibroblasts with or without LSP1P5 knocked down
To investigate the influence of LSP1P5 on H3K27me3 levels at the CEBPA promoter region in keloid-derived fibroblasts (KDFs), LSP1P5 knockdown was conducted using antisense oligonucleotide (ASO) in KDFs from two individuals. Subsequently, CUT&Tag assays were performed on both the knockdown groups (ASO-1) and the control group (ASO-NC). It is worth noting that initially, three ASO sequences were designed to target LSP1P5, and the original sequences, referred to as ASO2, showed the most significant effect. Therefore, this group was selected for the CUT&Tag assays, and the raw data file was labeled as ASO2. However, during the preparation of the manuscript, the original sequence name ASO2 was adjusted to ASO-1 for consistency in the published document.