Impact of bacterial derived microvesicles on gene expression of Caco2 cells.
Microvesicles (MEVs) were added on the Caco2 cells grown for 21 days on the insert until monolayer confluence. Triplicate samples for the MEVs treated (T1, T2, T3) and Triplicate samples for non-treated samples (C1, C2, C3) were processed for RNA extraction using Trizol method. Finally, RNA samples were DNase treated and cleaned with RNeasy MinElute Cleanup Kit (Qiagen) samples. Purified RNA was sequenced at Genome Québec (Montreal, Canada) and sequencing was performed using an Illumina NovaSeq PE100 sequencer. Overall design: MEVs were addred to the Caco2 monolayer after 21 days of growth on an insert and were allowed to be in communication with Caco2 for 24 hours. Following incubation, triplicate RNA samples for the MEVs treated (T1, T2, T3) and Triplicate samples for non-treated samples (C1, C2, C3) were sequence. C3 and T1 Samples are not included in the submission as the samples were not sequenced due to low quality RNA.