Genome-wide profiling of linker histone variant H1.3 in T47D-MTVL cancer cell line
Human somatic cells may contain up to seven members of the histone H1 family contributing to chromatin compaction and regulation of nuclear processes, apparently with certain subtype specificities. Our previous studies of H1.0, H1.2, H1.4, H1.5, and H1X genome-wide distribution determined that H1 variants are distributed in a variant-specific manner throughout the genome. We have been able to extend our study of the genomic distribution of the H1 family from five to six proteins thanks to the acquisition and validation of a new ChIP-grade endogenous antibody against histone H1.3. Overall design: Genome-wide analysis of linker histone variant H1.3 in T47D breast cancer cells (ChIP-Seq).