scRNA seq data from AML PDXs throughout a cytarabine treatment, and directly from AML patients at differnet stages of disease progression
We used scRNA seq on regenerating PDX models and de novo AML patient cells to delineate the heirarchical regeneration of AML following treatment Overall design: Bone Marrow from AML PDXs were isolated via mechanical dissociation and red blood cell lysis before FACS purification on humanCD45 and CD33 markers prior to scRNA seq protocol. De novo samples were purified by density gradient centrifugation and isolated for live cells via FACS 7AAD exclusion prior to scRNA seq protocol