Alternative platelet differentiation pathways initiated by non-hierarchically related hematopoietic stem cells
We performed single cell whole transcriptome (Smart-seq3) analysis of hematopoietic stem and progenitor cells long-term replenished by transplanted single Vwf-tdTomato+ P-HSCs (2290 total cells from 7 reconstituted mice) and Vwf-tdTomato Multi-HSCs (2478 total cells from 8 reconstituted mice), as well as control cells from normal mouse bone marrow.