Choclo virus (CHOV) recovered from deep metatranscriptomics of archived museum tissues
We extracted RNA and performed Illumina deep metatranscriptomic sequencing on archival tissues from a Costa Rican pygmy rice rat (Oligoryzomys costaricensis) collected in Panama that tested Orthohantavirus seropositive. Reads mapped to the Choclo virus (Orthohantavirus chocloense) reference were assembled and then validated by visualization of the mapped reads against the assembly.