Analysis of RNA expression in gastric tumor organoids
We generated organoids from mouse stomach epithelial cells which combination of C2mE (COX2 and mPGES1) expression (C), Wnt ligand expression (W), Kras mutation (K), Tgfbr2 loss(T), and mutant p53 expression(P). Interestingly, combination of Kras mutation and Tgfbr2 loss is important for invasion phenotype of stomach. On the other hand, Wnt1 ligand expression is important for metastasis phenotype. These results suggested that each gene mutation and gene mutation combination have a role of gastric cancer development.