Melanoma plasticity is regulated by mTOR-mediated crosstalk between MITF and TFE3
Bulk RNAseq was performed to identify differences in gene expression in SKMEL28 cells as related to MITF and TFE3 expression. Overall design: TFE3KO cell lines were created in the D2-MITFKO SKMEL28 and A375 background via CRISPR-cas9 strategies 500k SKMEL28: WT, D2 MITFKO, D2 MITFKO/TFE3KO (clone 3 and clone 13) and A375: WT and TFE3KO cells were plated in triplicate for biologic replicates. RNA was extracted utilizing RNeasy Plus Kit (Qiagen).