Homo sapiens Transcriptome or Gene expression
mTORC2-deficient human embryonic stem cell (hESC) lines have been generated by disrupting RICTOR gene that encodes the specific subunit of mTORC2. Although RICTOR-knockout (RIC-KO) hESCs can maintain self-renewal and the expression of pluripotent markers, RIC-KO considerably impairs cell adhesion and cell-cell interaction in hESCs by disturbing RhoA/Rac signaling pathways. The difference of transcriptomic profiles between WT and RIC-KO hESCs needs to be further explored.