Short-read genome sequencing for genotyping of rice tetraparental hybrids
Oryza sativa L. varieties: Nipponbare (NP), Kasalath (KS), IR26 and Taichung 65 (T65) were used as parental line for generating tetraploid from NP and KS (NPKS), and tetraparental hybrid from those four varieties (Quad). DNA was extracted from the leaves of these rice plants, and the obtained DNA sequences were used for genotyping of Quad lines.