Sample of bacteria on the surface of flue-cured tobacco Raw sequence reads
Samples were taken from a tobacco station in Weining County, Guizhou Province, China. The tobacco variety was yun-87, the picking site was central, the picking time was mid-July 2020, and the tobacco was cured directly after picking. Samples were taken at 0, 72, 120 and 168 h, corresponding to the fresh tobacco, yellowing, leaf-drying and stem-drying respectively. For each period, 5 leaves were selected and the base of the leaf and the main veins were cut off with sterilised scissors, wrapped in tin foil and snap frozen in liquid nitrogen (4 time points 5 repetitions). Wet and dry bulb thermometer readings as an environmental factor in the cured room at the time of sampling. The sequencing of the V3-V4 hypervariable region of 16S rRNA genes (primers: 5 -CCT ACG GRR BGC ASC AGK VRV GAA/T3 and 5 -GGA CTA CNV GGG TWT CTA ATC C-3).