DNase- and RNA-SEQ of Gynandropsis gynandra during de-etiolation.
G. gynandra is a C4 species closely related to A. thaliana and as such this study was designed to primarily understand the regulation of C4 photosynthesis establishment but secondly to compare this with an analogous data-set from A. thaliana. The aims of the study were to identify both shared and derived mechanisms of C4 pathway gene expression. Our understanding of C4 pathway gene regulation is largely limited to single genes and as such this whole genome approach sought to gain a broader overview of regulation while providing a valuable resource for further experimental work. G. gynandra seeds were germinated and grown in the dark for 3-days. Etiolated seedlings were then transferred to light to induce de-etiolation. Samples were taken at 0, 0.5, 2, 4 and 24 hours after first exposure to light. The 24 hour sample had been in the dark for 8 hours followed by 2 hours of light on day 2. The samples were processed for both DNaseI- and RNA-SEQ such that we could couple the chromatin accessibility and predicted binding events with mRNA abundance of the whole transcriptome.