Epigenetic regulation of Ucp1 by trandcription factor Etv4
We found a far regualtory element on Ucp1 genome locus at -12kb region which was drasticlly regulated by transcription factor Etv4. Overall design: ATAC-seq and H3K27ac, H3K27me3, CTCF cut&run was performed in BAT and iWAT tissue. ATAC-seq of -12kb enhancer repression by CRISPRi and knocking down Etv4 was performed in BAT cell line. RNA-seq of Etv4 knockdown was performed