Off-target deep sequencing of FLT3, CD123, KIT epitope-edited CD34+ HSPCs
Predicted off-target NGS sequencing of FLT3, CD123, KIT epitope-edited CD34+ HSPCs. Healthy donor-derived CD34+ HSPCs were cultured in vitro and epitope edited by means of SpRY_ABE8e + sgRNA electroporation. Target genes are FLT3, CD123, KIT, edited with FLT3-sgRNA-18, CD123-sgRNA-R, KIT-sgRNA-Y.