Microbiota analysis in pig milk
The purpose of this experiment was to clarify the microbiota in pig milk. The milk sample was collected from healthy sows (Landrace Ã? Yorkshire Ã? Duroc) which fed corn-soybean based diet. Milk samples were collected in a sterile tube and later suspended in MRS (Man, Rogosa and Sharpe) medium (200 ul). During milk sampling, sterile gloves were worn, nipple and the surrounding area were soaked with 75% ethyl alcohol to avoid the contamination by skin bacteria. The milk samples were immediately refrigerated at 4Â°C. After transportation, milk samples were dispensed into serum tubes, mixed with equal amount of 60% glycerol, and stored at -80 Â°C for future analysis.