Gut microbiota of 231 Japanese students aged 7-8, 10-11, and 13-14 years.
Feces samples of 231 healthy Japanese students were collected. The gut microbiome was analyzed by sequencing the 16S rRNA gene V1-V2 region amplified with 27Fmod and 338R primers (Kim SW. et al.). Equal amounts of each PCR amplicon were mixed and sequenced using Illumina MiSeq.