Single-cell-level gene expression and chromatin accessibility profiles of cells isolated from Drosophila embryonic gonads
We use scRNA-seq and scATAC-seq to investigate the development of germ cells and somatic gonadal precursors during embryogenesis. Overall design: For the scRNA part, the vas-GFP cells were collected from 8-12 and 12-20 hr embryos using Fluorescence-activated cell sorting (FACS) and then pooled together to generate a scRNA sequencing library. For the scATAC part, vas-GFP nuclei were isolated from 0-4, 4-8, 8-12, 12-16 and 16-20 hr embryos using FACS and then pooled together to generate both ATAC and snRNA sequencing libraries. To get an overview of the expression profiles of germ cells from the beginning of embryogenesis, we included our previous 0-8 hr scRNA-seq data (SRX8341666) here.