Aedes aegypti strain:Rexville D Transcriptome or Gene expression
Total RNA was extracted from each RexD pool using Trizol (Invitrogen). Small RNAs were isolated from the total RNA using the FLASHPAGE system (Applied Biosystems) and the manufacturer's recommendations. Individual sequencing libraries were prepared using the Applied Biosystem's Small RNA Expression kit. Use of bar-coded primers allowed library pools to be sequenced simultaneously on two slides. 18 libraries were sequenced by ligation based method using the SOLiD system (Applied Biosystems, Foster City, CA). Total reads per library ranged from about 12,000,000 to 49,000,000. Library construction included sRNA purification by size and required a free 5' monophosphate and 3' hydroxyl to allow ligation of adapters, therefore excluding capped mRNAs from library amplification.