A new HLA-C*07 allele
Here we describe a novel HLA-C*07  allele, identified in a a french  volunteer bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following: A*01:01 A*01:01  B*08:01 B*40:02 C*02:02 C*07:New   DRB1*03:01 DRB1*11:01  DRB3*01:01 DRB3*02:02 DQA1*05:01 DQA1*05:05  DQB1*02:01 DQB1*03:01    DPA1*01:03 DPA1*02:01      DPB1*01:01 DPB1*04:01 HLA-C*07 : New allele differs from C*07 :01  by one nucleotide substitution at position 217  of exon 2 (Posion 560 of cDNA) , where  base C is replaced by T Inducing Met instead  Thr (163th Codon ACG becomes ATG).