mutation during CRISPR/Cas
the effects of CRISPR/Cas9-induced double-strand DNA breaks on the repair processes of naturally occurring genome-wide mutations is unclear. After gRNA/Cas9 editing, these cells produced 859 mutations, including 67 high-impact variants that severely affected cellular functions under standard culture conditions.In contrast, gRNA/Cas9-edited cells under conditions of S~G2/M arrest and cyclin dependent kinase 1 inhibition showed only 5 mutations.