Transcriptome sequencing of Piper kadsura
Total RNA was extracted using the Illumina TruSeqTM RNA extraction kit, and concentration and purity were assessed using NanoDrop ND-2000. The integrity of total RNA was evaluated with an Agilent 2100 bioanalyzer. Qualified total RNA was utilized for sequencing library construction, and high-throughput sequencing was performed using Illumina NovaSeq.