Whole-transcriptome RNA sequencing and degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).
The maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV) were inoculated with SCMV (SC, SM) and phosphate buffer (MC, MM), respectively to subjected to whole-transcriptome RNA sequencing and degradome sequencing. Overall design: The crude extracts were inoculated on the second leaves of six-day-old Chang7-2 and Mo17 maize seedlings, and buffer-inoculated plants were used as control. At 10 days post inoculation, the systemically infected leaves from mock (MM, MC) and SCMV (SM, SC) maize plants with typical disease symptoms were collected respectively. In each treatment, the leaves samples from at least 15 maize seedlings were collected for RNA extraction.