Murine ESC-derived basal cell (iBC) 5th generation secondary transplantation
The goal of this experiment was to compare the transcriptome of iBC-derived lineages to endogenous tracheal epithelial cells after transplantation after serial secodnary transplantation. Overall design: Tracheas from two iBC 5th generation secondary transplantation were harvested 7 months post-transplantation. Tracheas were digested with a pronase-based method (Plasschaert et al., 2018), beforE FACS to separate GFP+ donor-derived cells and GFP- endogenous cells.