Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder
BBN is a bladder specific carcinogen that can cause invasive bladder cancer in mice possessing similar genetic alterations as seen in human cancer. We employed a single cell sequencing approach using 10x Genomics scRNAseq to investigate the cellular heterogeneity and molecular modifications induced by long term treatment with a carcinogen (BBN) on the bladder urothelium and sub-urothelium cells. Our data show infiltrations of B- and T-cells in the tumor and decrease of the several other urothelial populations. Overall design: 3 sample analysed: 2 non-treated controls and 1 BBN-treated. After 22 weeks of BBN treatment, the mucosa layer of mouse bladder was separated from detrusor and digested to prepare a cell suspension and single live cells were sorted using Fluorescence-activated cell sorting (FACS). Then scRNA-seq was performed to analyze the cells. The same was done for two age-matched non-treated mice.