High-throughput sequencing data of spacer acqusition
To detect the newly spacer sequence, the leader-proximal region of CRISPR array 1 was PCR amplified using Taq polymerase with forward primer Locus-F and reverse primer CRISPR1S4-R located at the 4th spacer of CRISPR array 1.The purified PCR products per sample was used for high-throughput sequencing.