MNase-seq data from triplicate induction experiments with pZEVi-LYS2 yeast
Paired-end sequencing of genomic DNA fragments purified from the MNase-digested chromatin of formalin-crosslinked yeast samples prepared in biological triplicate. Each biological replicate was treated with Beta-Estradiol (15nM or 1000nM) to induce pZEVi or left untreated (0nM) during growth at 30C in SC-Leu-Ura media that maintains Cas9/sgRNA episomes. The sgRNA was non-targeting in this strain background.