RNA-seq on primary human kidney epithelial cells with PPFIBP1 or PLEKHA1 knockdown
Here we investigated the transcriptomic changes induced by PPFIBP1 or PLEKHA1 siRNA treatments on human Primary Renal Proximal Tubule Epithelial Cells (RPTECs) with RNA-seq. Overall design: There are a total of 9 samples derived from 3 groups with 3 replicates per group. sint: RPTECs treated with non-targeting control siRNA (NTsiRNA). sipp: RPTECs treated with PPFIBP1 siRNA. sipl: RPTECs treated with PLEKHA1 siRNA. Libraries were generated with the ribosomal RNA removal method and sequenced with the NovaSeq 6000 S4 platform (2×150 bp) at a target of 30 million reads per library.