Identification of miRNAs and Their Target Genes in juvenile Nicotiana benthamiana Plants [degradome]
This study aims to identify and functionally characterize miRNAs and their target genes in juvenile Nicotiana benthamiana plants using miRNA sequencing (miRNA-seq) and degradome sequencing. miRNA-seq will be employed to profile the miRNA repertoire, while degradome sequencing will be used to identify miRNA-mediated mRNA cleavage sites and validate target genes. The combined approach will elucidate the regulatory roles of miRNAs in the early developmental stages of Nicotiana benthamiana and provide insights into their functional characteristics Overall design: The miRNA and degradome sequencing data from 21-day-old Nicotiana benthamiana plants