mRNA sequencing of human stem cell-derived hepatic stellate cells
Hepatic stellate cells (HSCs) activate upon liver injury and become significant contributors to the development of hepatic fibrosis, driving the progression of liver disease. HSCs derived from human pluripotent stem cells (hPSCs), termed scHSCs, provide relatively new models to help study HSC activation and fibrosis development. The dataset provides bulk mRNA sequencing data from scHSC lineages derived from multiple hPSC lines upon activation-inducing treatments, with the goal of studying HSC activation and providing insight into the scHSC model system.This dataset contains mRNA sequencing data of scHSC lineages derived from the following four independent hPSC lines:- WAe001-A (scHSC_1, WiCell Research Institute)- UCSFi001-A (scHSC_2, Corelli Institute for Medical Research)- WTSIi046-A (scHSC_3, Wellcome Trust Sanger Institute)- HMGUi001-A (scHSC_4, Helmholtz Zentrum Munchen).All scHSC lineages were subject to the following treatments for 48 hours:- Control: C (no treatment)- Starve: S (starvation of retinol and palmitic acid media supplementation)- TGFb: T (25 ng/mL transforming growth factor beta 1 (TGFb))- Starve + TGFb: ST (a combination of the Starve and TGFb treatments)