Pig-to-mouse fecal virome transplantation Raw sequence reads
Pig fecal samples (Pre-feces, Post-feces / bacteriome, virome)The bacteriome and virome of pre-weaning pig feces, used as the donor material for the pig-to-mouse model, were profiled using metagenomic sequencing.Pre-treatment mouse feces (Before antibiotics)Prior to any intervention, baseline fecal samples from recipient mice were collected and analyzed to characterize their native gut microbiota composition.Antibiotic-treated mouse feces (After antibiotics)Following broad-spectrum antibiotic treatment, fecal samples were collected from mice to confirm microbiota depletion and assess the post-antibiotic microbial state.Post-FMT and post-FVT mouse feces (Pre-, Post- / FMT, FVT)After administering either FMT (fecal microbiota transplantation) or FVT (fecal virome transplantation) using pig-derived material, mouse fecal samples were collected and subjected to metagenomic analysis to evaluate microbial and virome reconstitution and colonization.