Inference of secreted-protein signaling activities in intercellular communication
The SecAct (Secreted Activity) model infers secreted-protein signaling activity from spatial, single-cell, and bulk transcriptomics data. Using SecAct, LY86 was identified and validated in vivo as a promising immunotherapy target. Overall design: B16F10-Ly86 and B16F10-Vector tumors from mice that received 4 rounds of anti-PD-1 + anti-CTLA4 treatment were harvested and immediately snap-frozen in liquid nitrogen.