Cytosine base editors (CBEs) for inducing targeted DNA base editing in Nicotiana benthamiana
we constructed three cytosine base editors (CBEs) which contained different deaminases named A3A-CBE, A3A (Y130F)-CBE, and rAPOBEC1(R33A)-CBE, respectively, to compare their base editing efficiency in allotetraploid Nicotiana benthamiana (n = 4x). We used 14 target sites (T1 ~ T14) on the target NbPDS gene, and control (T0).Each experiment consisted of three biological repeats, and each result included two sequencing results.