Comparative analysis of the transcriptional response to bulky DNA damages
A systematic RNA-seq study comparing the transcriptional response to three independent bulky DNA damage inducing agents: UV, the chemotherapy cisplatin and benzo[a]pyrene, a component of cigarette smoke. It was performed in 2 different cell lines - GM12878 immortalized lymphoblast cell line and A549 lung cancer cell line. Overall design: We performed RNA-Seq in untreated GM12878 and A549 cells, as well as 6h after being treated with 5µM of BPDE, 200µM of cisplatin, 20J/m2 of UVC. For each condition there were 3 replicates: A549 control, A549 + cisplatin, A549 + BPDE , A549 + UV , GM12878 control, GM12878 + cisplatin, GM12878 + BPDE , GM12878 + UV.