New nul HLA-DQB1*04 allele
Here we describe a novel DRB1*04 allele, identified in a a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*02:01,*02:01 B*15:01,*40:01 C*03:03,*03:04  DRB1*04:New, *07:01 DRB4*01:01 *01:01P DQA1*02:01,*03:01 DQB1*02:02,*03:02  DPA1*01:03, *02:02 DPB1*01:01, *04:01   HLA-DRB1*04 : New allele differs from DRB1*04 :01 by one nucleotide substitution at position 97 of exon 2 (position 197 of cDNA), where  base A is replaced by T  inducing  aminoacid change (codon 37 TAT  becomes TTC  so Tyrosine 37 is changed by Phenylalanine )