Gut microbe of Bullfrog
Guangdong, Guangxi, Hunan, Hubei, Jiangxi, Fujian, Anhui, Hainan, Sichuan and Zhejiang. Sampling of bullfrogs was carried out in the above provinces, and one point was selected for sampling in each province. Six bullfrogs were randomly selected above 3 taels, dissected for intestinal contents, and about 2g of intestinal contents were mixed with 6 tubes and numbered for subsequent analysis of intestinal microorganisms and resistance genes. At the same time, two 1L bottles of water samples were taken back to the local laboratory for analysis. Temperature, PH, dissolved oxygen, and nitrite nitrogen were measured on-site with a YSI portable water quality analyzer, and the other indexes were brought back to the laboratory for measurement. After the water samples were fixed in the laboratory, the heavy metal contents of mercury, arsenic, copper, zinc and cadmium in the water body were determined.