Maternal adipose tissue DNA methylation and gene expression associated with excessive gestational weight gain
Omental adipose tissue was obtained from 24 women aged 25-40 years with a healthy singleton pregnancy and a pre-pregnancy BMI of 18.5-26 kg/m2. Genomic DNA was extracted for promoter DNA methylation analysis of candidate genes LEP, TNF, NPY, POMC and SOCS3 using bisulfite amplicon sequencing. Overall design: In a sample of 24 participants, we used a non-parametric regression to identify CpG sites with a methylation state that associates with