NRF1 promotes primordial germ cell development, proliferation, and survival [RNA-seq]
To investigate the role of NRF1 in regulating primordial germ cell development, We established conditional knockout mice of Nrf1 in primordial germ cell to observe the effect of Nrf1 knockout on the development of primordial germ cell. At the same time, we utilized a pluripotent stem cell differentiation system in vitro to obtain PGCL cells for chip_ Seq, analyze which genes Nrf1 directly binds to. Meanwhile, we established a pluripotent stem cell line induced by Nrf1 overexpression and performed RNA_seq analysis on PGCL cells overexpressing Nrf1 obtained in vitro Overall design: performed RNA-seq analyses on somatic cells (BV-) and PGCLCs (BV+) isolated from differentiated control PSCs and PSCs with enforced NRF1 expression