Effect of LLC1 tumor cell conditioned medium on RNA expression in mouse osteoblasts (MC3T3 cells)
MC3T3 osteoblasts were grown in culture medium in the presence of 10 mM beta-glycerophosphate and and 100 micro g per mL of ascorbic acid and   25% LLC1 conditioned medium or vehicle for 7 days. Cells were lysed an RNA was isolated. Overall design: The goal of the experiment was to determine the effect of LLC1 conditioned medium on MC3T3  cell RNA expression .  To this end, MC3T3 cells will grown in medium supporting mineralization.  Cells which treated with either LLC1 conditioned medium or vehicle for 7 days.  Cells were  collected, lysed an RNA was obtained for generation of libraries.