Direct RNA-seq ONT of blood stage Plasmodium falciparum
We used in vitro-cultured Plasmodium falciparum strains NF54 and DD2 and isolated RNA using Trizol for sequencing with ONT (500 ng input RNA, SQK-RNA002 kit, MinION flow cell (R9.4.1)).The following samples were collected:NF54: Rings, Trophozoites, Schizonts, Gametocytes stage V, Gametocyte stage V replicate from JHU &amp; Salivary Gland SporozoitesDd2: 10 hpi, 20 hpi, 30 hpi, 40 hpi, Committed Rings, Gametocytes stage I, II, III, IV, V &amp; V day 16In addition, we in vitro polyadenylated and sequenced the following samples using 150 ng input RNA: NF54 Trophozoites, NF54 Gametocytes stage V, Dd2 20 hpi, Dd2 Gametocytes stage V, Dd2 Gametocytes stage V day 16