Effect of recombinant vaccinia virus Tiantan strain on brain tissue transcriptome of Balb/c mice
To elucidate the biological significance of the GTPV N1L gene , we constructed three recombinant vaccinia virus Tiantan strain variants using homologous recombination: rVVT-N1L-deletion , rVVT-GFP , and rVVT-gN1L . The viral stocks were normalized to equivalent genome copy numbers and intracranially inoculated into 6-week-old female Balb/c mice at a dose of 4.23 * 10^5 viral copies in 100 Microlitre DMEM per mice. A control group received 100 Microlitre DMEM alone. On post-inoculation day 10, all mice were euthanized, and whole brains were harvested for transcriptomic analysis. To minimize individual variability, brain tissues from three randomly selected mice per group were pooled, and total RNA was extracted for Illumina-based RNA sequencing .