Transcriptome analysis of control and SKOV3 floating sphere cells and sphere differentiated cells
We initiated this study with the aim of systematically identifying the biomarkers driving tumorigenesis and maintaining tumor dedifferentiation in human ovarian cancer.  To achieve that goal, SKOV3 cells were seeded in ultra-low-attachment 96-well plates in DMEM/F12 medium supplemented with 2% B27 serum replacement to allow the formation of floating spheres.  Then, these spheres were collected and recultured in a 6-well plate in DMEM with 10% FBS to allow the cells in the spheres to differentiate and attach to the bottom surface, and the genes in which transcription patterns were altered during the transition from OCSCs to differentiated cells. Overall design: RNA-seq was performed in SKOV3-Adherent cells and SKOV3-stem cells with 3 replicates. Gene expression profiling was analyzed according to the sequencing results.