Effect of hypoxia on murine splenic NK cells
NK cells responses can be significantly affected by microenvironmental conditions. In pathological conditions, oxygen concentration in tissues can be reduced, lading to hypoxia. Here, we investigated how prolonged exposure to hypoxia affects the transcriptome of murine splenic NK cells. Overall design: Trascriptome profiling (RNA-seq) of murine NK cells isolated from spleen of C57BL/6 mice and cultured media supplemented with IL-2 in normoxia (20% O2) or hypoxia (1% O2) for 7 days