Transcriptomic and epigenetic analysis of adipose tissue in Cushing Syndrome [mouse]
Chronic glucocorticoids (GCs) exposure, resulting from endogenous Cushing Syndrome (CS) or prescribed GCs therapy, is associated with a high cardiometabolic burden. Moreover, previous studies have reported persistence of metabolic syndrome features after remission of hypercortisolism and in particular in visceral adipose tissue (VAT). Thus, in this study we want to analyze the transcriptomic alterations in VAT during active and cured CS and correlate these persistent gene expression changes with histone modifications induced by GCs. Overall design: RNA-seq (20 samples) and ChIP-seq (26 samples) of mouse visceral adipose tissue. CORT_5w means mouse model of active Cushing's syndrome, CORT_15w means recovered mice and VEH means controls.