Transcriptome analysis of immature pod in shatterproof soybean by inducing the nonsense mutations in four GmMYB26 genes
Reducing the yield loss of soybean (Glycine max) is critical to tackling resource depletion and climate change. Shatterproof soybean could be one of the solutions to the above issue, as pod shattering causes significant yield loss especially in arid climates. In this study, we developed a quadruple mutant of four GmMYB26 homologs that acquired significantly enhanced shatter resistance. We have performed RNA sequencing in quadruple mutant (shattering-resistant) and WT (shattering-susceptible). Total RNA was extracted from the immature pod (10 mm). We have identified differentially expressed genes (DEGs) and attempted to elucidate the effect of GmMYB26 genes on the gene-network of pod shattering.