Enrichment of redoxifibromiR miR-21-5p in Plasma Exosomes of Hypertensive Patients with Renal Injury
Experiment type: Non coding RNA profiling by high throughput sequencingSummary: The goal of this study is to investigate whether plasma exosomes from hypertensive and diabetes patients are enriched in fibromiRs and redoximiRs using deep sequencing technology.Overall design: Non-coding RNA sequencing analysis was used to determine miRNA in plasma exosomes in a discovery cohort of healthy subjects (n=25) and diabetic nephropathy patients (n=15). Candidate miRNAs were selected based on the differential expression between patient group and miR-21-5p was validated by quantitative polymerase chain reaction in a higher validation cohort.Relevance project: This novel study found an enrichment of fibromiRs and RedoximiRs in plasma exosomal biofluid from hypertensive/diabetic patients with renal dysfunction and are closely related with relevant signaling pathways implicated in fibrogenesis.