RNA-seq and ChIP-seq in Shanxinyang MYB156
Approximately 3 g of 4-week-old MYB156-MYC8 roots were cross-linked in 1 % formaldehyde under vacuum (10 min) and quenched with 0.125 M glycine for ChIP-seq. Total RNA was isolated from 8-week-old roots of WT, OE2, OE15, and MYC8 plants (three biological replicates) using the RNAprep Pure Plant Kit (DP441, TIANGEN)