Adenosine to Inosine RNA editing level in ADAR1-deficient or hypoxia-exposed human pulmonary artery endothelial cells
Hypoxia is a PH trigger which significantly downregulates ADAR1 and reduces A-to-(I)G editing in human PAECs. By RNA sequencing of PAECs after ADAR1 knockdown, we have identified the circadian gene Nocturnin (NOCT) as a direct ADAR1 target. NOCT carries two active A-to-I RNA editing sites in the 3'UTR. In addition, hypoxia significantly reduced A-to-G editing in human PAECs Overall design: To identify the genes regulated by ADAR1-induced RNA editing, RNA sequencing (RNA-seq) analysis was conducted in PAECs after ADAR1 knockdown. To investigate the effect of hypoxia on ADAR1-mediated RNA editing in hPAECs, we treated human PAEC with 48 hr hypoxia (1% oxygen) explosure. RNA sequence was perfermed and the overall A-to-I(G) RNA editing level was analyzed.