Metagenomics analysis of microbial diversity of rhizosphere of basmati rice( Oryza sativa L.)
The present study was carried out to study the microbial diversity of the rhizosphere of basmati rice to assess their functional potentials and to identify the microbial community influencing the aroma production. Metagenomic analysis of microbial diversity of the basmati rice revealed the most abundant microbial group associated with the rhizosphere were Actinobacteria, Proteobacteria, Enterobacteria, Burkholiderales, Rhizobiales, etc.. At phylum level, Proteobacteria being the most abundant microbial group present in the rhizosphere of Basmati 370 (46.43%) and non basmati rice variety SJR 5 (43.05%). The bacteria associated with the production of 2AP in basmati were Enterobacter, Actinobacteria, Burkholderia, Pseudomonas, Lactobacillus, Bacillus subtilis, Klebsiella, Micrococcus and Sinomonas. The enzymes Acetylornithine Aminotransferase, Acetylornithine Deacetylase, N-acetylornithine carbomyltransferase, Acetylornithine/succinyldiaminopimelate aminotransferase and Ornithine cyclodeaminase were involved in the biosynthesis of the potential precursors of 2 AP in basmati rice. Besides promoting 2 AP, these bacteria mostly belong to plant growth promoting bacteria (PGPB) group. Our study enriches the knowledge about the rhizobacteria responsible for 2AP production as well as PGPR activities and introducing these cultures into the basmati rice growing areas may enhance the 2AP. This will also enhance the export potential of the basmati rice in the region on sustainable basis.