Marine newly produced viruses and active bacteria by BONCAT (from the Pacific Ocean and the Mediterranean Sea)
This project includes raw sequencing reads (forward and reverse) from sorted (FACS) and MDA-amplified viral and microbial samples collected from the Pacific Ocean and the Mediterranean Sea. The samples were obtained using viral BONCAT-FACS, a method that combines bioorthogonal non-canonical amino acid tagging (BONCAT) with environmental sample incubation (incubation with 10 uM HPG for 5 days), followed by single-virus and single-cell sorting via flow cytometry (FACS).The dataset comprises raw reads from samples collected in both the Pacific Ocean and the Mediterranean Sea. For Pacific Ocean samples, both BONCAT-positive (active bacteria and newly produced viruses) and BONCAT-negative (non-active bacteria and pre-existing viruses) fractions are included. Click reactions (BONCAT reactions) lasted 6 hours to label newly produced viruses. For the Mediterranean Sea samples, only reads from the BONCAT-positive fraction (active bacteria and newly produced viruses) were sequenced. Two reaction times for the click reaction (BONCAT reaction) were tested: 30 minutes or 6 hours.Sample labels indicate:- The type of sample (cellular - bacterial - or viral),- The BONCAT activity status (active or inactive); for viruses, whether they are viral progeny or pre-existing viruses,- The origin of the sample: Pacific Ocean (PO) or Mediterranean Sea (MS).This project aims to provide detailed sequencing data to study newly produced marine viruses, active bacteria, as well as pre-existing viruses and inactive bacteria, across two major oceanic regions using cutting-edge BONCAT-FACS methodology.