Transcriptome analysis of CNS and splenic FOXP3+ Tregs vs. exFOXP3 T cells during EAE
EAE was induced in female FOXP3 fate mapping mice. At peak of disease, immune cells were isolated from brain and spinal cord (pooled as CNS) and spleen. Next, FOXP3+ and exFOXP3 T cells were FACS-sorted based on RFP and GFP expression. Overall design: FOXP3+ Tregs are sorted as GFP+RFP+ and exFOXP3 T cells as GFP-RFP+.