H3K27ac ChIP sequencing of low and high pigment B16 cells [B16_D5_LP_HP_ChIPSeq]
In one of our earlier studies, we have shown that, through a pH-mediated feed-forward loop, H3K27ac selectively activates pigmentation genes and accentuates melanocyte differentiation at the population level. Here we used the B16 progressive pigmentaiton model that harbours multiple melanocyte states in the same culture. We sorted the low and high pigment cells at day 5 and investigated the differences in H3K27ac mark between the low and high pigment melanocyte sub-populations. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modification H3K27ac in low and high pigment B16 cells sorted from the same culture.