Effects of an intestinal helminth infection of influenza virus immune cytotoxic T cells
Mice were infected with H1N1 influenza A virus and infected with Heligmosomoides polygyrus (HP) 21 days later. The mice were dewormed with ivermectin at day 38 and infected again with HP at days 46 and 70. At day 137, mediastinal lymph nodes, lungs and spleens were harvested. NP366-372+, PA224-233+, and NP366-372-PA224-233- CD8+ T cells were sorted by FACS. 10X 5' Gene expression and V(D)J libraries were prepared and loaded on on a NovaSeq S4 flow cell and sequenced on the NovaSeqTM 6000 system. Pre-processing of V(D)J reads and single cell RNAseq reads by with 10X CellRanger v. 7.0.0 was performed.