mRNA sequencing in crown tissue of barley sdw1 near-isogenic lines
We employed the mRNA-seq method to acquire transcriptome-wide characterization of the crown tissue and provide new insights into the expression profiles of heat- and gibberellin-related genes. The study accompanied application of ATAC-seq strategy to identify open chromatin regions (OCRs) and to characterize their distributions and organizations in crown tissues of three barley (Horedeum vulgare) genotypes carrying different allelic forms of the sdw1 gene encoding Gibberellin 20-oxidase (GA20ox) subjected to high temperature. ATAC-seq and RNA-seq data integration provided results concerning relationships between chromatin openness and gene expression changes.