Stromal vascular fraction inhibits inhibits kidney fibrosis and inflammation in UUO mice
To understand the roles and mechanisms of stromal vascular fraction (SVF) in renal fibrosis induced by unilateral ureteral obstruction (UUO), we performed RNA sequencing of UUO kidneys from PBS- or SVF-treated mice. The transcriptome analysis revealed that SVF triggered significant metabolic reprogramming and improved mitochondrial function. Moreover, SVF treatment inhibited kidney inflammation as revealed by the downregulation of immune cell migration pathway. Furthermore, SVF contributed to the activation of PPAR signaling while inhibited TGF-beta signaling pathway. Overall design: Bulk RNA sequencing was performed in UUO kidneys from PBS- or SVF-treated mice that were subject to UUO surgery.