Histone 3 lysine 9 acetylation-specific reprogramming regulates esophageal squamous cell carcinoma progression and metastasis
Dysregulation of histone acetylation is widely implicated in tumorigenesis, yet its specific roles in the progression and metastasis of esophageal squamous cell carcinoma (ESCC) remains unclear. We here profiled the genome-wide landscape of H3K9ac for paired adjacent normal (Nor), primary ESCC (EC) and metastatic lymph node (LNC) esophageal tissues from three ESCC patients. By integrating these data with the corresponding H3K27ac profiles, we identified a distinct epigenetic reprogramming specific to H3K9ac in EC and LNC samples compared to Nor samples, which predominantly targeted genes functionally associated with tumorigenesis and metastasis, and contributed to their transcriptomic aberrations. The findings were further verified by a publicy available single cell RNA-seq data and in vitro experiment, highlighting the potential therapeutic avenues for intervening ESCC through epigenetic modulation via H3K9ac. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) of H3K9ac for paired adjacent normal (Nor), primary ESCC (EC) and metastatic lymph node (LNC) esophageal tissues from three ESCC patients.