scRNA-seq of adult Mus musculus intestinal immune cells
Four C57BL/6 genotypes (wild type, Bmal1-/-, Apc+/-, and Apc+/-;Bmal1-/-) were used to study how circadian disruption in the intestine affects the tumor microenvironment of colorectal cancer. Adult mouse intestines were collected, digested for single cell suspension, and then FACS sorted for Cd45+ immune cells. scRNA-seq was performed on these immune cells and analyzed.