Nanopore amplicon sequencing of ITS regions for the detection of Phytophthora infestans in potato leaves
This project aims to detect Phytophthora infestans in inoculated and healthy potato leaf tissues using Oxford Nanopore Technologies sequencing of ITS1-ITS4 amplicons. Samples were collected at different timepoints from two potato cultivars to assess the feasibility of this approach for quantifying the relative abundance of the pathogen.