4-OI treatment inhibit LPS-induced neutrophil inflammatory responses
Our in vitro cell research has revealed that LPS stimulation can induce the upregulation of the immune response gene 1 (Irg1) in neutrophils. Itaconic acid is a metabolic derivative generated by Irg1,4-octyl itaconate, which is exogenous itaconate, can exert protective effects in acute colitis.  we utilized RNA sequencing-based omics analysis to explore the impact of 4OI treatment on the activation of inflammatory responses in neutrophils and the underlying mechanisms.Transcriptomic analysis revealed that 4-OI suppresses inflammatory signaling pathways including NF-?B and Toll-like receptor pathways.The experiment was divided into 3 groups, including the control group, the LPS stimulation experimental group, and the 4-OI treatment group. Overall design: RNA-seq profiling of bone marrow-derived Wide type neutrophils were activated by LPS, and 4-OI was added to inhibit the inflammatory response.