RNA-Seq of EIF4A3 knockdown in human embryonic kidney cell line HEK293 via siRNA
EIF4A3 is one of the three core components of the exon junction complex (EJC). The EJC is deposited during pre-mRNA splicing upstream of the exon-exon junction and serves as a mark for exon boundaries. The EJC is involved in various gene expression regulatory mechanisms, such as nonsense-mediated decay (NMD). NMD is a translation-coupled mechanism that targets mRNAs harboring a premature stop codon (PTC) for degradation, thereby serving as a quality control and gene regulatory pathway ensuring transcriptome integrity. Here we performed an siRNA-mediated knockdown of EIF4A3, followed by RNA-Seq in order to explore the transcriptome-wide expression changes including the extent of NMD inhibition. As control, siRNA targeting the non-expressed Luciferase was used.