Metagenomic sequencing of the Intestine microbiota in Periplaneta americana
n this study, we employed metagenomic sequencing to analyze the Intestine microbiota of Periplaneta americana. We propose that when specific bacterial species dominate a given environment, their corresponding phages typically exhibit proportional abundance. Using a metagenomic approach, we simultaneously acquired comprehensive datasets of both the host microbiota and associated phages. By analyzing the quantitative relationships between hosts and phages, we identified the most dominant phage types in the Periplaneta americana gut, enabling targeted isolation and subsequent experimental validation.