Gene expression alterations with Nkapl knockout or NKAPL frameshift mutation (M349fs)
This study aims to explore the transcriptomic changes in Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mouse testes comparing with their wild-type testes. Fresh testes were collected from 21d wild-type, Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mice, respectively. Overall design: For each group, we have biological triplicates. Total RNA was extracted using TRIzol reagent. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.