CCSER1 transcriptomic analysis reveals a role in neurodevelopmental phenotypes and alcohol response.
CCSER1, a gene with limited prior knowledge in neurological conditions has emerged as a potential tumor suppressor and regulator of neurodevelopment. Our analysis reveals that CCSER1 downregulation correlates with down regulation of DNA repair and DNA replication pathways and up regulation of neurodevelopmental pathways, including calcium signaling and neuroactive ligand-receptor interaction, in CCSER1-deficient zebrafish. These findings suggest a potential role for CCSER1 in neurodevelopmental processes and its impact on adult behavior, as evidenced by altered alcohol preference in zebrafish models. Further investigation is needed to elucidate the precise molecular mechanisms underlying CCSER1 function and its contribution to human diseases, such as addiction and neurodevelopmental disorders. Overall design: We generated CRISPR/Cas-9 mutants in the gene CCSER1 - CCSER1_P471 or CCSER1_M, and CCSER1_S48Y* or CCSER1_S. For analysis of trancriptomic changes in adult zebrafish whole bodies and brains, homozygous mutants were raised to adulthood. WT controls were siblings from the heterozygous incross. RNA-seq analysis performed on whole brains and of whole body without the brain. Sample QC and sequencing was outsourced. An mRNA cDNA library made up of Paired-end sequencing reads of 150 bp in length was generated on the Illumina HiSeq instrument.