Bulk RNA-sequencing analysis of isogenic murine Kras/p53 (KP) Brg1 wildtype cells versus Brg1 knockout (KPB) cells
The goals of this experiment is to identify transcriptomic changes upon loss of Brg1 in lung adenocarcinoma cells. Overall design: Kras/p53 lung adenocarcinoma tumor derived cell lines were subjected to CRISRP/Cas9-mediated genome editing using guides against sgTomato (control) or sgBrg1 (sample). Isogenic Kras/p53 mutant lung cancer cell lines with and without Brg1 were derived and used for bulk RNA-sequencing. Experiment was performed in triplicate conditions.