Enhancing radiotherapy response via intratumoral injection of the TLR9 agonist CpG to stimulate CD8 T cells in an autochthonous mouse model of sarcoma
p53/MCA sarcomas are induced by intramuscular injection of adenovirus expressing Cas9 and sgRNA targeting Trp53 (Adeno-p53-sgRNA; Viraquest) at gastrucnemius muscle site. We used scRNA-seq to analyze the differences in immune cells after treatment with CpG+RT