gut microbiota and metabolites
The aim of this study is to investigate the impact of tacrolimus on the gut microbiota of LT patients. Patients were grouped based on the duration of tacrolimus use, whole blood drug concentration, and concentration-dose ratio. The Illumina HiSeq X platform was employed to detect the gut metagenome, analyzing the composition and characteristics of the gut microbiota. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology was used to measure gut microbiota metabolites and reveal their metabolic profiles.