Sequencing-based quantification of Erwinia amylovora strains
We developed and utilized an amplicon sequence (AmpSeq) based approach to identify SNPs between strains to investigate strain - strain interactions in vitro and in planta. This approach was able to successfully identify and quantify strains in a mixture. In vitro assays showed that E2002a exhibited the fastest growth and outcompeted the other strains (Ea273 and E4001a) as cultures approached the death phase.