Systematic identification of long noncoding RNAs during three key organogenesis stages in zebrafish
Thousands of lncRNAs have been found in zebrafish embryogenesis and adult tissues, but their identification and organogenesis-related function have not elucidated. In this study, high-throughput sequencing was performed at three different organogenesis stages of zebrafish embryos, which were important for zebrafish muscle development. The three stages were 10 hpf (hours post fertilization) (T1), 24 hpf (T2) and 36 hpf (T3). Overall design: To investigate the function of lncRNAs during organogenesis stages in zebrafish. We then performed gene expression profiling analysis using RNA-seq during three key organogenesis stages (10, 24, 36hpf). Differentially expressed lncRNAs were screened out and lncRNA gas5 was selected as the next research target.