Comprehensive Physiological, Transcriptomic and Metabolomic Analysis of Exogenous Trehalose Regulating Cold Tolerance in Grape Callus
This study aimed to investigate the physiological and transcriptomic responses of grape to low-temperature stress under exogenous trehalose treatment. Key genes and pathways responsive to cold stress were identified through transcriptome analysis combined with WGCNA and metabolomics, to explore the molecular mechanism by which exogenous trehalose regulates cold tolerance in grape.Methods Wild-type grape callus was used as material and treated with exogenous trehalose. After exposure to 4 degrees C for 10 days, phenotypic changes were observed and physiological and biochemical indices were determined. Transcriptome sequencing was performed on samples from different treatments, followed by screening of differentially expressed genes DEGs, functional enrichment analysis, weighted gene co-expression network analysis WGCNA, metabolomic analysis, and RT-qPCR validation.Conclusion Exogenous trehalose positively regulates the defense response of grape to low-temperature stress. In this study, specific modules and core genes associated with low-temperature tolerance in grape were identified via transcriptome combined with WGCNA and metabolomics. These findings support the application of exogenous trehalose in improving cold tolerance of grape and may provide potential targets for breeding cold-resistant grape varieties.