RNA-Seq of flow-sorted Egfr WT or Egfr KO pancreatic stellate cells (PSCs) and flow-sorted pancreatic tumor organoids following co-culture.
We investigated changes in transcriptome in Egfr KO PSCs co-cultured with pancreatic tumor organoids compared to Egfr WT PSCs co-cultured with the same pancreatic tumor organoids. We also investigated the changes in transcriptome in pancreatic tumor organoids co-cultured with Egfr WT or Egfr KO PSCs. Overall design: 23 samples in total. Comparative gene expression profiling analysis of RNA-seq data for flow-sorted Egfr KO PSCs co-cultured with pancreatic tumor organoids compared to flow-sorted Egfr WT PSCs co-cultured with the same pancreatic tumor organoids. 12 samples were analyzed (n=4 WT; n=8 KO). Comparative gene expression profiling analysis of RNA-seq data for flow-sorted pancreatic tumor organoids co-cultured with Egfr WT PSCs or Egfr KO PSCs. 11 samples were analyzed (n=4 WT; n=7 KO).