Effect of PD0325901 on gene expression during inner ear organoids differentiation
To determine the mechanism through which PD0325901(PD) promotes inner ear hair cells generation, we cultured the inner ear organoids in 1 µM PD or the same volume of DMSO for 10 days, after which we used RNA-seq to establish a database to analyze differentially expressed genes . Overall design: To determine the mechanism through which PD0325901(PD) promotes inner ear hair cells generation, we cultured the inner ear organoids in 1 µM PD or the same volume of DMSO for 10 days, after which we used RNA-seq to establish a database to analyze differentially.