Bulk RNA sequencing of OSCC patients derived cells
Tumor samples from OSCC patients were dissociated into single cells. Patient derived cell lines were grown in their standard culture conditions. Then the cells were harvested and dissociated into single cell suspensions. The cDNA library preparation was performed using the BD Rhapsody Express Single-cell analysis system (BD Biosciences) following the manufacturer's instructions. All quantifications during the procedure were done using Qubit (ThermoFisher Scientific) and 2100 Bioanalyzer system (Agilent) and finally pooled in the ratio-spheroid mRNA Library: Sample tag library: 2.91%:97.09%. Pooled libraries were sequenced (150 bp pair-ended) on the Illumina HiSeq X Platform. The scRNA sequencing of head and neck cancer derived patient cells were done from different patients to check the differential gene expression profile among them.