16S amplicon sequencing of soil microbiota under saline-alkali land improvement
Soil salinization is a major environmental constraint limiting agricultural productivity. In this study, we aimed to investigate the microbial community dynamics during saline-alkali land improvement using high-throughput amplicon sequencing. We performed 16S rRNA gene sequencing for bacterial community analysis. Soil samples were collected from saline-alkali treatment plots under different improvement strategies. The objective was to identify core microbiota associated with soil amelioration and to elucidate their potential ecological functions. This study provides insights into the microbial mechanisms underlying saline-alkali soil restoration and identifies key microbial taxa that could serve as bioindicators or bioinoculants for future agricultural applications.