Transcriptome data of PaoP5-dap1 overexpression in PAO1
PAO1/p-dap1 overexpression was established, and the control group PAO1 and the experimental group PAO1/p-dap1 were cultured until OD600 reached 0.6. The bacteria were collected by centrifugation, immediately frozen with liquid nitrogen. Three biological repeats were performed. RNA extraction and RNA-seq analysis were performed as described earlier. The SV Total RNA Isolation System was used to isolate the total RNA, and the RNA quality and quantity were checked using Bioanalyzer and RNA 6000 Nano kit. Then, rRNA was removed using the Ribo-Zero rRNA removal kit, then the cDNA libraries were constructed and sequenced on an Illumina HiSeq 2500 sequencer. Gene expression changes were measured by the Reads Per Kilobase Per Million Read (RPKM) and the false discovery rate (FDR) (q value). Differentially expressed genes (DEGs) between PAO1 and PAO1/p-dap1 culture groups were calculated by DESeq, and genes with a fold change value of >2 and a q value of 0.05 were determined as DEGs.