c-Kit+ cells that intercalate with crypt Lgr5+ cells are distinctively multipotent in colonic epithelium renewal and repair
c-Kit+ cells  mark deep crypt secretory cells (DCSs) and supply Wnt3, EGF, and Notch signals to support their neighboring crypt bottom-intermingled Lgr5+ cells. We used single cells sequencing (scRNA-seq) to analyse the diversity and stemness of  c-Kit+ cells in the colonic epithelium. Overall design: The whole colonic epithelium cells were isolated from c-Kit-creERT2 mice, and c-Kit+ cells derived cells (GFP+ cells) of c-Kit-creERT2;Rosa26-mTG mice isolated by Fluorescences-activated cells  cell sorting (FACS) according to prescense and absence of GFP signal and analysed using scRNA-seq.