Bulk RNA-seq of sham or alkali injury treated WT and Vegfr3 depletion mouse iris
We report conditional ablation of lymphatic Vegfr3 alleviated inflammatory process in alkali injured iris. Prox1CreERT2 mice were crossed with Vegfr3fl/fl to selectively target gene expression in Prox1+ lymphatic cells. Injury models were established at 6 weeks postnatally, and Vegfr3 gene depletion was conducted via Tamoxifen injection on the day 13th-17th after corneal alkali injury. Mice were sacrificed 28th days post-injury. Overall design: To evaluate the pathological impact of Vegfr3 in alkali-injured mouse iris, we performed bulk RNA-sequencing analysis from PBS-treated Sham Vegfr3fl/fl mice, corneal alkali injury-treated Injured Vegfr3fl/fl and Injured Vegfr3fl/fl;Prox1CreERT2 mice. We conducted three experiments (3 identical genotype samples in each group).