Non-canonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen
Non-canonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen Overall design: Th17 WT (5 mice total, combined from two separate experiments) and Th17?STAT3 (4 mice total combined from two separate experiments) were immunized with MOG(35-55) in CFA to induce EAE. On day 10 post-immunization dLN were harvested and CD4+YFP+ Th17 cells were sorted on a FACSAria II, directly into SmartSeq low-input RNA kit lysis buffer. Libraries were prepared using Nextera XT DNA library prep kits, RNA-Sequencing was performed on Illumina NextSeq500 by the Health Sciences Sequencing Core at University of Pittsburgh.