Whole genome bisulfite sequencing data of blood cfDNA form 29 CRC and 9 Non-CRC patients.
The goal of this study is to develop a low-cost method to quantitively detect the DNA 5mC modification at single base resolution with 1 ng blood cfDNA bypassing next-generation sequencing. We sequenced blood cfDNA samples form 29 CRC and 9 Non-CRC patients using whole genome bisulfite sequencing and analyzed the data. We extracted DNA methylation sites and developed a model using 8 DNA methylation sites based on previous reports for the validation of this method. We then apply our method, LAMP-MASS (Linear Amplification-Mass spectrometry) to detect the DNA methylation status of the 8 sites combination. The accuracy of LAMP-MASS method is similar as whole genome bisulfite sequencing or genomic-region specific bisulfite sequencing. Moreover, LAMP-MASS method prevails because of low cost, user-friendly control and convenient data processing.