Childhood cancer mutagenesis caused by a domesticated DNA transposase
Induction of somatic oncogenic mutations by the domesticated DNA transposase PGBD5 in cerebellar progenitor cells promotes medulloblastoma development. Overall design: Medulloblastomas from Ptf1a-Cre/+; Ptch1fl/fl; Pgbd5+/+ (n=3) and Ptf1a-Cre/+; Ptch1fl/fl; Pgbd5-/- (n=3) mice were collected and sequenced for single-cell Multiome. The tumor onset age (harvested age) varied from 94 to 239 days.