Transcriptomes of the venom gland of Agelena limbata
To explore the diversity of toxin information of Agelena limbata Thorell and to obtain toxin-related gene sequences, so as to provide theoretical basis for further study of spider venom. High throughput sequencing technology was used to sequence the transcriptome of the venom glands of Agelena limbata, and bioinformatics method was combined to analyze the transcriptome. A total of 43 157 unigene genes were obtained by sequencing, and the homology was the best with Stegodyphus dumicola by Nr annotation. The proportion of GO-enriched protein complexes and immune system processes is the highest. KEGG database has the highest proportion of global and overview. The results of comparison in Uniport database showed that the main functions of proteins in poison glands were antibacterial, insecticidal, blocking nerve pathways, blocking sodium ion channels, etc. T/A, AT/TA and TG/CA appeared most frequently in SSR loci. The venom glands of Agelena limbata contain abundant toxin genes, which provide a source of transcriptome data for subsequent studies.