WGS of a serie of Arabidopsis thaliana tetrad's products to study the role of RECQ4A, RECQ4B, FIGL1 and FANCM genes in the rate, nature and distribution of meiotic recombination products (CO and NCO)
In this study, two divergents accessions of Arabidopsis thaliana (Columbia and Landsberg erecta) were crossed and tetrads were obtained using the quartet mutation, which keeps the 4 haploid pollen grains from a single meiosis attached together. By fertilising a plant (of ecotype Columbia) with a single tetrad, then selecting seeds from siliques containing exactly four seeds and finally sequencing the 4 developed plants, we can access the complete history of meiotic recombination events occurring during a single male meiosis. The analysis of WGS tetrads data consists of genotyping a series of SNV markers (differentiating Columbia and Landsberg) positioned on the five chromosomes for the 4 (M1, M2, M3, M4) individuals of a tetrad, representing the 4 chromatids of each chromosomes. A total of 20 tetrads, the F1 and the two parental accessions Columbia and Landsberg erecta were sequenced.