Amplicon sequencing of carbon-fixation gene (cbbM) from soil samples in Horqin Sandy Land
These sequences represent the cbbM gene (encoding ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, form II) amplified from soil samples under sand-fixation plantations in the Horqin Sandy Land, Inner Mongolia, China. Soil samples were obtained from a herbaceous vegetation succession sequence (shifting sand, early, middle, and late successional stages). The cbbM gene fragments were amplified using specific primers and sequenced on the Illumina MiSeq platform. This study investigated the diversity and structural changes of autotrophic microbial communities capable of CO2 fixation via the Calvin cycle in response to vegetation succession in semi-arid sandy soils. These sequences provide valuable data on the distribution and ecological functions of form II RuBisCO-containing microorganisms in restored desert ecosystems.