Chemically defined, serum-free and xeno-free generation of mature type 2 alveolar epithelial cells from hiPSCs
HiPSCs were differentiated into alveolar type 2 cells (AT2s) following a stepwise differentiation protocol, mimicking lung development, using a chemically defined, serum-free, and xeno-free differentiation protocol. Samples contain biological triplicates of each developmental stage (hiPSCs, definitive endoderm, lung progenitor stage, passage 0 AT2s, early and late passage AT2s) using the same cell line. Additionally, a biological triplicate for a cell line from an idiopathic pulmonary fibrosis patient with a homozygous surfactant protein C mutation was done for late-passage AT2s comparison.