16S rRNA amplicon sequencing of salinized agricultural drainage sediment from Alar, Xinjiang, China
This project contains paired-end Illumina NovaSeq 6000 16S rRNA V3-V4 amplicon reads generated from 16 surface sediment (0-20 cm) samples collected in late March 2024 from salinized agricultural drainage channels in Alar, Xinjiang, China. Sediment DNA was extracted using a modified CTAB protocol, the bacterial 16S rRNA V3-V4 region was amplified with primers 338F/806R, and libraries were sequenced using PE250 chemistry. The dataset was generated to characterize bacterial community composition and to examine relationships between dissolved organic matter characteristics, salinity gradients, and bacterial community assembly in agricultural drainage sediment.