Gene profile of human monocyte-derived macrophages treated with MTX
Comparison of the transcriptome macrophages derived from CD14+ human monocyte-derived macrophages generated in the presence of M-CSF (M-Mphage) or GM-CSF (GM-Mphage) and MTX. Overall design: mRNA profiles of monocyte-derived macrophages generated by culturing monocytes for 7 days in the presence of M-CSF (10 ng/ml) to generate M-Mphage, or in the presence of GM-CSF (1000 U/ml) to generate GM-Mphage, while treated with 50nM MTX.