The iPSC-derived pulmonary epithilial cells in micro-patterned culture plate [CUT&Tag]
We established a system of generating alveolar or airway organoids in a micro-patterned culture plate. In this system, various lung cell types were identified, depending on the culture condition. Overall design: Human iPSC-derived lung progenitor cells were seeded onto micro-patterned plates and cultured in "DCIK+3i" (CHIR99021, SB431542 and Y27632) medium for 7 days to induce alveolar organoids involving alveolar type 2 cells in the DCIK+3i medium. Thereafter, the alveolar organoids were cultured in the following three conditions for 7 days: "DCIK+3i" to maintain alveolar type 2 cells, "DCI+LATS(LATS-IN-1)" to induce alveolar type 1 cells, or "PAL" to induce alveolar transitional state cells (iATCs), respectively. The organoids in each condition were dissociated using Accutase and subjected to CUT&TAG analysis.