Studying sequence specificity of manikomycin A action by Ribo-seq
Manikomycins (MKMs) are a family of newly discovered depsipeptides active against Gram-negative bacteria. MKMs inhibit bacterial translation through the interaction with the E site of the large ribosomal subunit. To investigate if MKMs display any sequence specificity of action we performed ribosome profiling (Ribo-seq) experiment for Escherichia coli cells treated with MKM A in parallel with untreated control cells.