RAVER1 interconnects TGFB signaling, EMT and miR/RISC activity by modulating alternative splicing [A549_RAVER1_KD]
The depletion and deletion of RAVER1 substantially impairs the 2D-proliferation of tumor cells, vitality in 3D cell models and the growth of subcutaneous xenografts in nude mice. Disturbance of RAVER1 affects various cancer hallmark pathways manifesting in deregulated E2F/DREAM-controlled transcription and broadly hampered microRNA-dependent gene silencing. In carcinoma-derived cell models, impaired control by microRNAs (miR) leads to the upregulation of various tumor suppressors like BTG2, which is suppressed by the most abundant oncomiR 21-5p. Overall design: Poly-A RNA-seq of lung cancer-derived A-549 cells, transfected with either control siRNAs (siC) or siRNAs directed against RAVER1 or PTBP1. Three biological replicates per condition