Genomic alterations in Gastric cancer of Naga Tribes: Clinical and prognostic implications
A detailed analysis of the molecular causes of Gastric cancer in the Naga Tribes was conducted. The Next Generation Sequencing method also known as Whole Exome Sequencing (WES), Chain termination sequencing or Sanger sequencing of the CDH1 gene, and ALU indel markers CD4 and D1 were used to find mutant variants in samples and assess the prognosis of the condition. The ALU indel markers analysis revealed significant associations between CD4 genotypes and Gastric cancer cancer, particularly in female patients, with a greater prevalence of the DD genotype. The effects of the CD4 and D1 polymorphisms on cancer prognosis were also examined in this study. The CDH1 gene's Sanger sequencing provided new information about     E-cadherin  and how it affects the development and spread of Gastric cancer. Patients with Gastric cancer were found to have genetic alterations discovered by whole exome sequencing, including new mutations in genes like PLEKHG5 and RUNX1, which offer important information about the pathogenesis of the disease and possible treatment options. The results of this investigation have improved our knowledge about stomach cancer. The ALU indel markers CD4 and D1 were the main focus of the thesis's genetic analysis of Gastric cancer patients from the Naga Tribes. To find mutant variations and assess the prognosis of the disease, whole exome and Sanger sequencing of the CDH1 gene were used. The study discovered strong relationships between the frequency of Gastric cancer and the CD4 marker, emphasising in particular that the DD allele is more common in female cases. The function of CD4 polymorphisms and its correlation with prognosis and cancer risk were also covered. Significant connections between male genotypes were found in the study, which focused on the genetic basis of cyclin D1's cytoplasmic position. The significance of the CDH1 gene in cell-to-cell adhesion and the role of its dysfunction in the genesis and spread of cancer were identified by Sanger sequencing study. Whole exome sequencing revealed alterations linked to the onset and prognosis of cancer in a number of genes, including PLEKHG5 and RUNX1. The results highlighted the intricacy of genetics pertaining to Gastric cancer and its consequences for treatment outcome and prognosis. As a result, the study opened up new possibilities for future investigation and treatment approaches by shedding light on the molecular pathways behind Gastric cancer among the Naga Tribes. The Alu indel markers CD4 and D1 were investigated in order to find genetic markers and mutations associated with stomach cancer in the Naga Tribes. The study employed Chain terminating sequencing of the CDH1 gene and WES to analyse  mutant variations and estimate the prognosis of the illness. The frequency of the DD allele was the emphasis of the research, which revealed a significant correlation between patients CD4 marker and their disease, particularly in female patients. Furthermore, in order to shed light on the relationship between cancer risk and prognosis, the study investigated the functions of the D1 genotype and CD4 polymorphisms in gastric cancer. The significance of E-cadherin in cell-to-cell adhesion and its role in the advancement of cancer were highlighted by the Sanger sequencing analysis, which concentrated on the CDH1 gene. By identifying mutations in many genes linked to Gastric cancer by whole exome sequencing, possible biomarkers and their consequences for treatment response were clarified. Notably, the identification of PLEKHG5 and a new mutation in the RUNX1 gene demonstrated their potential as prognostic markers and factors in the advancement of tumours. The Naga Tribes' thorough investigation of genetic markers and mutations linked to Gastric cancer has shed important light on the disease's molecular causes. The results enhanced our knowledge of the genetic underpinnings of Gastric cancer and have implications for personalised medicine with regard to both diagnosis and treatment. The discovered biomarkers and genetic changes acted as a basis for further studies in the area and could lead to the advancement of more efficient methods for the detection, assessment, and management of  Gastric cancer.