Effects of ZAKalpha on UVB-dependent trancriptional changes in N/TERT-1 cells
This study investigates the effect of ZAKalpha kinase in UVB-triggered signaling in an immortalized keratinocyte cell line N/TERT-1. The experiments compared sham-irradiated and UVB-irradiated isogenic Cas9 control and ZAK KO cells in order to define which UVB responsive transcripts are controlled by ZAKalpha. Overall design: Isogenic Cas9 control and ZAK KO N/TERT-1 cells are either sham irradiated or UVB-irradiated (100mJ2/cm2). All cells were harvested 4 hours after irradiation.