MicroRNAs of the larvae of Toxocara canis
The goal is to assess the impact of host serum on the larvae of Toxocara canis miRNA in vitro. Larvae were cultured in RPMI 1640 medium without serum or with 5% serum prepared from helminth free mice for three days. Small RNA sequencing libraries were constructed using a TruSeq Small RNA Sample Prep Kit, following the manufacturer instructions. A single end sequencing was performed on the Hiseq 2500 system. A total of 8 miRNAs libraries were prepared.