LH/hCG regulation of circular RNA in mural granulosa cells during the periovulatory period in mice
To investigate the role of circular RNA in the granulosa cells at two different stages of gonadotropin stimulation RNA was isolated from ovarian granulosa cells followed by highthroughput sequencing and circRNA anlaysis Overall design: Granulosa cells were isolated 46 hours after PMSG injection and 4h after hCG injection (PMSG primed mice) were used for RNA isolation and high through put sequencing