Fecal gut microbiome of maternal and nursey pigs fed hydrolyzed yeast
Ninety primiparous sows (initial BW 189.2 kg) were enrolled on d 85 of gestation and assigned to one of three maternal diets: a control (M0) or diets with 0.25% (M0.25) or 1.0% (M1.0) hydrolyzed Saccharomyces cerevisiae fed until weaning. At weaning (d 21), 24 litters per maternal treatment were assigned to either a nursery diet without yeast (N0) or with yeast (NY; 0.75%, 0.50%, and 0.25% in phases 1-3, respectively). Offspring from M1.0 litters were only assigned to N0 (n = 12). Fecal swab samples were collected and flash frozen using liquid N from sows on d 21 (final weight averaged 186 kg) and from the same piglet per litter on d 21 (final weight averaged 5.5 kg), 28 (averaged 6.7 kg), and 66 (averaged 27.9 kg) with balanced sex representation. Feces was homogenized using the Qiagen Tissuelyzer II, then microbial DNA was extracted using the DNeasy 96 PowerSoil Pro Kit. Afterwards, the V3-V4 region of the 16S was amplified and then sequenced on an Illumina NextSeq 1000.