Transcriptome profiling of starved Actr3 KO mIMCD3 cells
Actr3 WT (control), Actr3 KO and Actr3-Ift88 double KO mIMCD3 cells were generated using CRISPR/Cas9. Single cells clones (Actr3 Ctrl.-1, Actr3 KO-1, Actr3-Ift88 dKO) were selected and validated by western blotting confirming loss of Actr3 or Ift88 in KO cell lines on protein level. Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis of three independent replicates of starved (ciliated) Actr3 Ctrl.-1, Actr3 KO-1, Actr3-KO Ift88-KO (dKO) and 0.5 µM Ciliobrevin D treated Actr3 KO-1 cells was performed. Overall design: mRNA sequencing analysis of control, Actr3 KO, Actr3-KO Ift88-KO (dKO) and 0.5 µM Ciliobrevin D treated Actr3 KO-1 mIMCD3 cells