TCRb-seq of mouse CD8+ T cells isolated from Cd4-Cre and Ch25hfl/flCd4-Cre mice following VSV-OVA infection
To analyze TCR diversity, CD8+ T cells specific to the OVA (257-264) peptide-H-2Kb tetramers were isolated from Cd4-Cre and Ch25hfl/flCd4-Cre mice following VSV-OVA infection. The KLRG1+CD127– effector cell population was subsequently sorted, and total RNA was extracted from the cell samples using TRIzol reagent.