Transcriptomics of heat stress response in Ganoderma lucidum
This project investigates the transcriptional response of Ganoderma lucidum mycelia to heat stress. Mycelia were cultured using semi-submerged static culture on CYM medium under controlled laboratory conditions and subjected to heat stress at 42 degrees Celsius for 30 minutes. Samples were harvested immediately after treatment (0 days, HS-A) and three days post-heat stress (3 days, HS-B). Control samples were grown under identical conditions without heat stress. Paired-end RNA-seq libraries were prepared using PolyA selection and sequenced on the Illumina NovaSeq 6000 platform. Biological replicates (n=3 per condition per time point) were included to ensure reproducibility. This dataset provides a comprehensive resource for analyzing differential gene expression and understanding acute and delayed transcriptional responses to heat stress in G. lucidum.