RNA-Seq analyses of H2O2+LaCl3 treated leaves and roots of barley (Hordeum vulgare cv. GoldenPromise)
To figure out the transcriptional nature of the Ca2+-dependent H2O2 response, we made a comparative analysis between genes obtained with H2O2 +LaCl3 treatment versus differentially expressed genes obtained with only H2O2 treatment. LaCl3 is a plasma membrane channel blocker, which is known to hinder the stimuli-induced Ca2+ signatures in plants.