Effect of Low-Fat Cheddar Cheese Supplementation on the Gut Microbiota of Human Microbiota-Associated Mice
This project investigates the impact of dietary low-fat Cheddar cheese on the gut microbiota using a human microbiota-associated (HMA) mouse model. Germ-free C57BL/6 mice were colonized with human fecal microbiota and fed either a control diet or a diet supplemented with low-fat Cheddar cheese for six weeks. Fecal samples were collected longitudinally and analyzed using 16S rRNA gene (V4 region) amplicon sequencing to characterize changes in microbial community composition and diversity associated with cheese supplementation. This dataset supports analysis of diet-microbiome interactions in a controlled experimental model.