#OE-MdARF5
The 8th-day embryonic leaves of the WT and MdARF5-overexpressing lines cultured in EIM were analyzed via RNA sequencing, with three replicates. The high-quality Illumina sequences were compared with the apple reference genome (https://iris.angers.inra.fr/gddh13). DESeq2 software was used for DEG analysis and visualization. Then, heatmaps and Venn diagrams were drawn on the basis of the results of the DEG data analysis. Kyoto Encyclopedia of Genes Genomes (KEGG), Gene Ontology (GO) and Clusters of Orthologous Groups for Eukaryotic Complete Genomes enrichment analyses were used for functional annotation of the DEGs.