RNA-seq data of MCF7 human breast cancer cells with altered JAM-A expression
Recent studies have shown that transmembrane-type tight junction proteins (TJPs) are upregulated in various cancers compared to their levels in normal tissues and are involved in cancer progression. Here, we investigated the expression profile and a novel role of junctional adhesion molecule-A (JAM-A), one of the transmembrane-type TJPs, in breast cancer. Immunohistochemistry of surgical specimens showed that JAM-A was highly expressed from carcinoma in situ lesions, as in other adenocarcinomas, with higher expression in invasive carcinomas. High expression of JAM-A contributed to malignant aspects such as lymph node metastasis and lymphatic involvement positivity. In MCF7 human breast cancer cells, JAM-A knockout and rescue experiments revealed that JAM-A expression status affected malignant potentials, including proliferation and migration. To uncover these mechanisms, we conducted RNA-seq of MCF7 cells with altered JAM-A expression.