Studying the microbiome of LCN2-treated allo-HCT recipients
BALB/c recipient mice were lethally irradiated and transplanted with allogeneic bone marrow and T cells from C57BL/6 mice. All recipients were treated with vehicle or LCN2 on day 7 after transplantation. Stool samples were collected on day -1 and on day 14 after transplantation and DNA was isolated. Variable region 3 and 4 amplicons of the 16S rRNA gene were sequenced according to Illumina protocols.