Mus musculus Transcriptome or Gene expression
The construction of effective animal models of human HCC is important for studying the pathogenesis of liver cancer and clinical drug development. We used diethylnitrosamine (DEN) (Macklin, 55-18-5, China) to induce liver cancer in mice, which simulates pathogenesis of human liver cancer.We randomly divided 69 5-week-old male C3H/HeN mice into two groups, with 33 and 36 mice in control group and DEN group, respectively. The control group was injected intraperitoneally with 0.9% normal saline twice weekly, and the DEN group was injected intraperitoneally twice weekly with a dose of 50 mg/kg (body weight) DEN to construct a liver cancer induction model. Both control and DEN experiments were performed at the same time and lasted 7 weeks.