Transcriptome analysis of Sogatella furcifera after treatment with plant growth regulators
The R6834 Total RNA Kit I was used to extract tissue RNA from WBPH under the stress of three plant growth regulators (PGRs). Quality inspection was performed using Nanodrop, Qbuit and electrophoresis. The library was constructed according to the operation of VAHTS Universal V6 RNA-seq Library Prep Kit for IlluminaQ. After library quality control using Qubit3.0 and Q-PCR, double-end sequencing was performed on the Illumina Novaseq 6000 platform. The filtered data was aligned to genes using STAR software, and the differentially expressed genes between groups (WCK versus WBR, WC, and WP) were analyzed with DESeq2 software. We used qPCR to verify the consistency of 10 significantly differentially expressed genes with transcriptome sequencing results. CNCI and AnimalTFdb database were used to identify lncRNA and TFs in transcripts. KOfam and GO were used for functional annotation of genes and TFs