Effects of renal ischemia-reperfusion injury in Azin1 A-to-I locked and uneditable mice
We generated two mouse models of Azin1 A-to-I editing. In the first model, the editing site is locked in the edited state (AGC serine to GGC glycine). In the second model, the editing site is disrupted while preserving the codon composition (AGC serine to TCC serine). Bulk total RNA-seq was performed on kidney tissues under basal conditions and 24 hours after 20 min bilateral renal ischemia-reperfusion injury. Overall design: Ischemia-reperfusion injury was performed under isoflurane anesthesia. Prior to surgery, mice were given extended-release buprenorphine at a dose of 3.25 mg/kg. The mice were subjected to a 20-minute bilateral renal pedicle clamp followed by reperfusion. Tissues were harvested 24 hours after surgery. A heating pad was used to ensure their rectal temperature remained above 36 °C throughout the surgical procedure. No antibiotics or fluid resuscitation were administered.