Saccharum trigalactosyldIacylglycerol 5 and nac-transcription factor 108 Mutant Line Sequencing
This study aimed to compare non-sequencing methods of genotyping CRISPR-Cas9 mediated mutations at six target single guide RNA sites within the LIGULELESS 1, TRIGALACTOSYLDIACYLGLYCEROL 5, and NAC-TRANSCRIPTION FACTOR 108 loci in sugarcane (var. CP88-1762).