RNA-Sequencing analysis of control and UXS1 KO A549 cells
The mRNA profiles of A549 cell line were analyzed by RNA-sequencing under control and UXS1 KO conditions to identify gene expression changes induced by disruption of UXS1. Overall design: Compare the mRNA profiles of A549 cancer cells under CTRL (non-targeting guide) and UXS1 KO mediated by CRISPR-cas9. Each condition was analyzed in biological triplicate for a total of 6 samples.