Identification of a new DQB1*06 allele in a a french  voluntary bone marrow donor
Here we describe a novel DQB1*06 allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*01:01,*-  B*57:01,*38 :01 C*06:02, *12:03 DRB1*07:01, *13:01 HLA-DRB3 01:01 HLA-DRB4 01:03N DQA1 01:03, *02:01 DQB1*03:03 DQB1*06:New DPA1 02:01, *02:02 DPB1*01:01, *13:01  HLA-DQB1*06 New allele differs from DQB1*06:03 :01 :01  by one nucleotide substitution at position 65  of exon 2 (position  174 of cDNA), where  base T is replaced by C , Without aminoacid change (25Leu  CTT>CTC).