Multiplexed Sequencing of Expression Cassette Expressing Human ASL in Yeast
Multiplexed sequencing of yeast yASL expression cassette. yASL encodes the human ASL protein, using a codon-optimized derivative of the human ASL cDNA sequence. Derivatives of yASL were introduced into haploid yeast strains so that each encoded a version of human ASL with one amino acid changed. Multiplexed sequencing using the MinION platform was used to determine the altered codon, and associated amino acid change, in each yeast transformant