RNA sequencing in Atp7b -/- mice under high-fat diet conditions
The aim of this study was to elucidate the impact of ATP7B deficiency on hepatic gene expression profiles in mice fed a high-fat diet. Systemic ATP7B-knockout (Atp7b-/-) mice on a C57BL/6J genetic background were generated via CRISPR/Cas9 technology and subjected to a high-fat, high-fructose, and high-cholesterol diet (abbreviated as high-fat diet, HFD) for 24 weeks to establish a steatohepatitis model. Hepatic gene-expression profiles were analyzed by RNA sequencing (RNA-seq). ATP7B deficiency exacerbated hepatic inflammation and fibrosis in HFD-fed mice, as revealed by RNA-seq data. Overall design: Liver gene expression signature were compared between wild-type (WT) and Atp7b-/- mice at 24 weeks of HFD feeding.