RNA-seq analysis of EpCAM-GFP and GFP overexpression in FLO-1 esophageal adenocarcinoma
EpCAM is one of the highly expressed marker in esophageal adenocarcinoma as identidied in several multi-OMICS platform. We utilize FLO-1 cells which are lacking in EpCAM expresssion and use overexpression strategy to elucidate the function of EpCAM in this cells. Recombinant EpCAM-GFP or GFP only was trasnfected in FLO-1 cells, and the stable cells after antibiotic selection were isolated and expanded. The cells were then subjected to mRNA sequencing. Overall design: The stable FLO-1 cells overexpressing EpCAM-GFP or GFP were subjected to RNA-seq analysis