16sRNA sequencing of intestinal microbiota  in Mytilus coruscus after starvation and refeeding
Subject mussels were treated in two periods, a starvation period: 0 days of starvation (S0), 7 days of starvation (S7), 30 days of starvation (S30) and a refeeding period: S7F7 (7 days of refeeding after 7 days of starvation), S7F30 (30 days of refeeding after 7 days of starvation), S30F7 (7 days of refeeding after 30 days of starvation), S30F30 (30 days of refeeding after 30 days of starvation). Intestinal microbial DNA extraction, bacterial 16S RNA amplification, and sequencing were performed .