soil metagenome
In this study, we investigated the effects of 5 mM Fe2+ and Mn2+ coexisting in varying ratios on the denitrification ability of SAD in terms of S and N products by conducting batch experiments with S0 as the electron donor. Additionally, we analyzed changes in microbial community structure and relative functional gene expression to examine the response patterns between microbial community structure, related functional gene expression, and S and N products, thereby clarifying the potential mechanisms. The Xingchang Wastewater Treatment Plant in Zhejiang, China, provided the sludge that was used to enrich and cultivate SAD-associated microorganisms.