Transcriptomic analysis of estrogen-treated MCF-7 breast cancer cells
This project investigates the involvement of MAPK/ERK and PI3K/AKT signaling pathways in estrogen-mediated regulation of mitochondrial fission in MCF-7 human breast cancer cells using RNA sequencing. Cells were treated with 10 nM 17beta-estradiol for 24 and 48 hours, with ethanol-treated cells as controls. Total RNA was extracted and sequenced on the Illumina NovaSeq 6000 platform. Differential gene expression analysis was performed to identify estrogen-responsive genes and pathways. Selected targets were validated by quantitative PCR.