Effects of high cholesterol diet on sperm  small RNA expression profile utilizing two different RNA-seq methods
Purpose: To  reveal the small RNA profiles in the sperm using traitional and PANDORA-seq methods Methods: 3 week old LDLR-/- male mice were fed a high cholesterol or low cholesterol diet for 9 weeks. Sperm RNA was extracted via Trizol. Results: paternal HCD feeding can lead to sperm tsRNA/rsRNA landscape changes that are undetected by traditional RNA-seq Overall design: Sperm small RNA were sequenced from LDLR-/- male mice after 9 weeks of low cholesterol or high cholesterol feeding