Soil Raw sequence reads
This study investigated the impacts of different soil amendments on the structure and succession of **bacterial** communities in agricultural soil. Soil samples were subjected to three treatments: 1) biochar amendment (Emb), 2) imidacloprid pesticide application (Imid), and 3) a mature compost amendment (Ma), with an untreated control group (Con). Samples were collected at multiple time points (7, 14, 28, and 58 days) post-treatment, with three biological replicates per group. The **V3-V4 hypervariable region of the bacterial 16S rRNA gene** was amplified using primers **338F and 806R** and sequenced on an Illumina Nextseq 2000 platform to profile the bacterial microbiota. The objective was to assess how these common agricultural practices alter bacterial diversity, composition, and keystone taxa over time, which has implications for soil health and ecosystem functioning. This dataset comprises the raw sequencing reads (fastq files) for all samples.