Single cell RNA-seq analysis of Drosophila pole cells of embryos produced from pgc/pgc mothers overexpressing importin-alpha2.
We isolated pole cells from embryos produced from pgc/pgc; EGFP-vasa/+ females overexpressing importin-alpha2 mated with y w males at 2-3 h AEL, and performed single cell RNA-seq to examine heterogenous gene expression in the pole cells.