Single-cell epigenomic analysis of hypodermal macrophages from Csf1r-cre x Igf1-flox mice.
To explore the effect of cell-autonomous Igf1 expression on hypodermal macrophages, these cells were isolated from Csf1r-cre x Igf1-flox mice and subjected to single-cell ATAC-sequencing. Overall design: Skins from Csf1r-cre x Igf1-flox and Igf1-flox mice were harvested and the hypodermal layers isolated by mechanical force and enzymatic treatments. Cells were liberated after enzymatic digestion of the tissues. Macrophages were isolated  by fluorescence-activated cell sorting (FACS) and their chromatin accessibility assessed by single-cell ATAC-sequencing using the 10X genomics plateform.