Transcriptome profiling of OCM-1A melanoma cells stably expressing Mycobacterium tuberculosis Rv0183 and its catalytically inactive S110A mutant
This transcriptome dataset was generated to investigate the functional role of the Mycobacterium tuberculosis protein Rv0183, which possesses monoacylglycerol lipase activity. The Rv0183 gene fragment was amplified from the Mycobacterium tuberculosis genome. A site-directed mutation was introduced to replace the serine residue at position 110 with alanine (S110A), a substitution known to abolish its enzymatic activity. Both the wild-type Rv0183 and the catalytically inactive Rv0183 (S110A) mutant were stably expressed in the OCM-1A melanoma cell line. Following stable expression, transcriptome sequencing was performed to compare the transcriptional profiles associated with each construct.