biofilm Raw sequence reads
Silver nanoparticles (AgNPs) are widely present in the environment and have an important impact on the structure and ecological function of environmental microbial communities due to their strong biological effects. Electroactive microorganisms play an important role in biogeochemical processes and are important forces driving metal-mineral transformations and elemental geochemical cycles in nature. At present, the laws and molecular mechanisms of the effects of long-term exposure to AgNPs on the extracellular electron transfer (EET) of electroactive microbial communities are not clear. In this study, we propose to use microbial fuel cells (MFCs) as a research platform to investigate the effects of long-term exposure to AgNPs on EET through quantitative studies of biofilm electroactivity of MFC anodes; to elucidate the changes in the diversity of electroactive microbial communities under the influence of AgNPs with the help of multidimensional histological techniques (macro-genome, macro-transcriptome and macro-proteome), and to reveal the core active species driving the communities The molecular mechanisms (regulation of key pathway genes and proteins) of AgNPs affecting the metabolism and biological oxidation of electroactive microbial communities were elucidated; finally, the interactions between AgNPs and electroactive microorganisms and the mechanism of nanotoxicity were elucidated at the cellular level with the help of pure bacteria exposure experiments and pure bacteria physiological and biochemical analysis, and by comparing the results with those at the community level, the reveal the community effects of electroactive microorganisms. This study will help to further understand the microbial toxicity of nanopollutants and assess their effects on biogeochemical processes.