Effect of 4sU labeling durations on quantification bias in nucleotide conversion RNA-seq data
To investigate the effect of labeling durations on quantification bias in nucleotide conversion RNA-seq, we labeled NIH cells with 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples. Overall design: Increasing labeling durations in NIH cells for up to 2 hours in 2 replicates.