Whole-genome sequencing of lactic acid bacteria isolated from chicken litter.
Fresh chicken feces were obtained from the Robert T. Hamilton Poultry Teaching &amp; Research Facility at Iowa State University. Following collection, feces were homogenized in sterile saline, diluted, and spread-plated on DeMan, Rogosa, and Sharpe (MRS) agar. Plates were incubated in an air-tight box containing Anaero-Packs (Mitsubishi) to create an anaerobic environment. Bacterial isolates were propagated and initially identified with 16S rRNA sequencing. Three isolates identified as Ligilactobacillus salivarius were selected for whole-genome sequencing using Oxford Nanopore Technology.