Transcriptome analysis of Colletotrichum tofieldiae strain constitutively expressing CtBOT6
To investigate the whole fungal transcriptional changes induced by CtBOT6 in Colletotrichum tofieldiae 4 (Ct4), we performed RNA-seq analysis on CtBOT6 constitutive expression transformants (OX#1, OX#10, OX#24) compared to Ct4 WT. To obtain candidate genes regulated by CtBOT6, we employed in vitro growth conditions in which we can detect the synthesis of the botrydial precursors in the OX lines while excluding any potential influence from plants. Three-day-old mycelia cultured in liquid media were used for RNAseq analysis.