Third-party reconstruction of Matryoshka RNA virus 1 from public African RNA-seq
This study describes the third-party reconstruction and annotation of Matryoshka RNA virus 1 (MaRNAV-1) genomic sequences from publicly available RNA-sequencing datasets generated from Plasmodium vivax samples of African origin, specifically Ethiopia. Consensus viral sequences were produced by reference-guided mapping of RNA-seq reads to previously published MaRNAV-1 reference genomes. Viral open reading frames, including the RNA-dependent RNA polymerase (RdRp), were annotated based on conserved sequence features and comparative analysis with existing INSDC records. No primary sequencing was performed for this study; all analyses relied exclusively on public data, and sequence provenance is explicitly documented. The resulting annotated consensus sequences are intended to facilitate comparative genomic, evolutionary, and epidemiological investigations of MaRNAV-1 in African Plasmodium populations.