Genes regulated by pharmacological Wnt activation by CHIR99021 in the embryonic mouse cochlea
Our studies showed that Wnt activation influenced patterning of the mouse cochlea across the radial axis. To identify genes responding to Wnt activation, mouse embryonic cochleas were treated in the presence and absence of the GSK 3beta inhibitor, CHIR99021. Cochleas were harvested on E12.5 and cultured in vitro for 24 hours. On the next day (day 1), cochleas were treated with either DMSO vehicle control or CHIR99021. The 6-hour treatment helped to identify the immediate genes transcribed following Wnt activation. Overall design: Embryonic day 12.5 cochleas were cultured for 1 day and then were treated with vehicle (DMSO) or CHIR99021 for 6 hours. Bulk RNA-seq Includes 4 biological replicates from Control and CHIR treatment.