gene expression profiles of Col1a1+Vldlr+ and Col1a1+Vldlr- TSPCs
We here report Reln-VLDLR receptor-ligand interactions are the primary mechanism for communication between lymphatic endothelial cells (LECs) and tendon stem/progenitor cells (TSPCs). The depletion of Vldlr results in impaired tendon regeneration.Specifically, lymphoangiocrine Reelin interacts with Col1a1+Vldlr+TSPCs to activate Notch1-Srebf2 mediated cholesterol metabolism loop, maintaining TSPC stemness and enhancing tendon regeneration. Overall design: To explore the role of Vldlr+ cells in tendon regeneration, we sorted Col1a1+Vldlr+ and Col1a1+Vldlr- TSPCs by flow cytometry.We then comparare gene expression profiling analysis of RNA-seq data for Vldlr+ and Vldlr- TSPCs.