Microglia response upon demyelinating injury [ATAC-seq]
To understand the impaired transcriptional regulation of microglia/macropahges in aging following demyelinating injury. Transcriptional regulation requires the repositioning of the nucleosomes around promoters and enhancers, thereby making chromatin accessible to transcription factors. Overall design: ATAC (Assay for Transposase -Accessible Chromatin) sequencing was performed on CD11b+ cells isolated from the white matter of young and old mice at 4dpi after lesion induction.