Longitudinal gene expression profiling in Nodal-induced and control zebrafish embryonic explants
We report RNA-sequencing from zebrafish explants generated from embryos injected with mRNA encoding the Nodal ligand ndr2 (N), constitutively active Nodal receptor CA-acvr1b* (T), and uninjected (U) controls. Explants of all 3 conditions were compared at each of 7 developmental stages: sphere (SR), 30% epiboly (30), 50% epiboly (50), shield (SH), 75% epiboly (75), 90% epiboly (90), and 2-somite (2S) (corresponding to 4, 4.7, 5.3, 6, 8, 9, and 11 hours post fertilization, respectively). Overall design: Sequencing of polyadenylated mRNAs from ndr2 (N), CA-acvr1b*(T), and uninjected (U) explants with 3 biological replicates (A-C) per condition per stage (SR - 2S).