scRNA-seq of 50 and 80 hpf isolated zebrafish hearts
Seeking to identify additional transcription factors required for cardiac valve formation, we determined and explored the transcriptional landscape of endocardial cells at the time when key morphogenetic events underlying valve development take place. Overall design: We isolated wild-type zebrafish hearts at 50 hours post-fertilization (hpf) when valve identity has just been established, and at 80 hpf when forming valves are first observed