Gene Expression Alterations in KDM6A-Knockout Renal Cell Carcinoma Cells
To investigate the functional role of KDM6A in renal cell carcinoma (RCC), we established KDM6A-knockout (KO) cell lines by employing CRISPR/Cas9-mediated disruption of the KDM6A gene in 786-O and Caki-1 wild-type (WT) cells. Comparative RNA sequencing analyses were performed to identify molecular pathways associated with KDM6A deficiency. Total RNA was extracted from both KDM6A-KO and WT control cells and processed into libraries using the SureSelect Strand-Specific RNA Library Preparation Kit (Agilent Technologies). Transcriptome analysis was conducted with the HiSeq 2500 next-generation sequencer (Illumina), and the resulting sequence reads were aligned to the human genome (hg38). The RNA sequencing dataset, including samples from KDM6A-KO 786-O, KDM6A-KO Caki-1, WT 786-O, and WT Caki-1 cells, will be publicly available.