Extension of Project PRJEB30101 - BMMF2 from human mothers´ milk
Samples of human mother's milk from 12 different mothers were analyzed for the presence of Bovine Meat and Milk Factors (BMMF1 and BMMF2). Only one sample contained BMMF2 DNA sequences, whereas all samples tested negative for a subgroup of BMMF1 tested. However, seven BMMF2 full-length DNA genomes, as well as a small circular BMMF2 DNA (496bp) molecule were isolated from a milk sample obtained from a single mother. The genomes of 5 of 7 the full length BMMF2 isolates and the small circular isolate are almost identical to the C2MI.5A.1 which is the full-length genome of one of our original isolates C2HB2 obtained after fractionation of density gradients of bovine serum (Funk et al., 2014, de Villliers et al., 2019).