Integrated metabolome and transcriptome analysis of fulvic acid relieves nitrate stress-induced damage in spinach by regulating multiple defense pathways
In the current environment of agricultural production, abiotic stress caused by high nitrate is becoming more and more serious. Fulvic acid (FA), a plant growth regulator, acts a vital role in improving plant stress tolerance. However, specific impacts of FA on vegetables under nitrate stress have been less studied. This study focused on spinach as the research object, changes in growth physiology, gene expression, and metabolites of spinach under nitrate stress by 0.15% FA application were investigated by analyzing physiology, transcriptomics, and metabolomics. The results indicated that FA could alleviate the adverse impacts of nitrate stress on spinach growth and chlorophyll synthesis, inhibit the accumulation of ROS in leaves caused by nitrate stress, the increase in malondialdehyde (MDA) content and relative conductivity (REC).