RNA-seq in A549 cell lines.
Purpose: The goals of this study are to invsetigate the primary biological process and signalling pathway of HNRNPD. Results: Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis of the differential genes showed that the PI3K signaling pathway and HIF-1 signaling pathway were enriched after HNRNPD knockdown. Overall design: MRNA profiles of NC cells and HNRNPD knockdown cells were used. Briefly, samples (A549 cells transfected with negtive control or HNRNPD siRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform (Seqhealth Co., Wuhan, China).