Erigoster B Targeting DECR1 Induces Ferroptosis of Breast Cancer Cells via Promoting Phosphatidylcholine - Arachidonic Acid Metabolism
Breast caner, the leading cause of death in woman, is profoundly affected by coopreative changes of molecular and pathological characters in fatty acids metabolism. Transcriptome sequencing was performed to investigate the role of DECR1 in modulating molecular changes of breast cancer cells, we used MDAMB231 and BT474 cell lines with stable knockdown of DECR1. RNA was isolated with TRIzol reagent according to the manufacturer instructions. Briefly, cells were lysed with TRIzol directly in the culture dish. The lysate was transferred to a microcentrifuge tube, and chloroform was added. After vigorous shaking and centrifugation, the aqueous phase containing RNA was collected and mixed with isopropanol to precipitate the RNA. Following centrifugation, the RNA pellet was washed with ethanol, air-dried, and resuspended in RNase-free water. The concentration and purity of the RNA were measured using NanoDrop. The transcriptome sequencing library was constructed using the RNA Library Prep Kit for Illumina. Next, the library was sequenced on the Illumina NovaSeq platform. Raw reads in fastq format were processed. Then, the read count of each gene was calculated by the featureCounts package.