Methylation data of Chinese Spring leaves
We used enzymatic methyl-sequencing (EM-Seq) to detect DNA methylation patterns at single-base resolution in hexaploid wheat. Genomic DNA was extracted from the flag leaves of Chinese Spring wheat, and libraries were prepared using TET2+APOBEC3A enzyme and PCR amplification. Sequencing was performed at an average depth of 14x. This results demonstrate that the EM-Seq approach is cost-effective and accurate for studying DNA methylation in plants with large and complex genomes.