Ovarian sequencing of Group A3 and Group N
Total RNAs from ovaries in A3 and N groups were extracted. The concentration and integrity of total RNA were detected by Nanodrop 2000 and Agilent 2100, respectively. The qualified RNAs were sequenced using the Illumina platform. The filtered Reads were aligned with the rabbit genome (Oryctolagus_cuniculus. OryCun2.0) using HISAT2 (http://ccb.jhu.edu/software/hisat2/index.shtml). DEGs were screened with |log2Fold Change|>1 and P-value<0.05 as the threshold.