ChIP-Seq profiles of HIM-1, CSR-1, COH-3 and H3K9me2 at adult stage in C. elegans
Chromatin fractions of C. elegans were prepared from adult populations by formaldehyde cross-linking and sonication. The worm strain was any one of N2, unc-11(e47) dpy-5(e61) I; sDp2(I;f), or him-8(tm611) IV. Chromatin immunoprecipitations were performed with antibodies against any of HIM-1, CSR-1, COH-3/4, or histone H3K9me2. Libraries of DNA co-immunoprecipitated with the antibodies as well as DNA of input lysates were sequenced using SOLiD5500 sequencer in single-end read mode. The average fragment length for these libraries was approximately 245 bp. SOLiD-compatible software should be used for sequence alignment.