biocompatibility results for endovascular medical device
In this project, we developed an endovascular medical device. To evaluate its biocompatibility, the device (experimental) or a clinical medical device (control) was co-cultured with human immune cells. We further conducted single-cell RNA sequencing to examine potential immunogenic responses at the cellular level. Overall design: We employed an in vitro co-culture model to compare immune responses to the developed device and a reference clinical medical device. Briefly, devices were incubated with human immune cells under controlled culture conditions for a defined exposure period. After co-culture, freshly collected samples were immediately transported for library preparation and processed using a standard single-cell workflow. The resulting single-cell profiles were analyzed to identify differences in cell composition and gene expression patterns. The integrated dataset was further processed using established pipelines for dimensionality reduction, clustering, and comparative analysis.