Paracrine effect of fibroblasts on the proliferation and differentiation of bovine satellite cells in vitro
The co-culture of fibroblasts and muscle cells is essential to obtaining cultured meat using in vitro techniques. Recent studies indicate that fibroblasts support the process of myogenesis and regeneration of damaged muscle fibers through cell-cell interactions. However, little research has focused on the fibroblast paracrine effect on myogenesis and transcriptome changes. This study aims to examine the paracrine effect of fibroblasts on the myogenesis process, based mainly on transcriptome study. Studies were conducted at the stage of proliferation and differentiation of muscle cells. RNA-Seq analysis revealed changes in many genes involved in the myogenesis process at the proliferation and differentiation stages – such as upregulation of EGR1, IL6, and SOCS3 and downregulation of ITGA7. The co-culture of muscle satellite cells with fibroblasts at the proliferation stage promotes myogenic differentiation, but at the differentiation stage, the culture conditions set by the type of medium (differentiation medium) have a prevailing effect on the course of myogenesis over the paracrine effect of fibroblasts. Overall design: RNA Sequencing analysis of bovine muscle satellite cells cultured in co-culture with fibroblasts, compared to muscle satellite cells monoculture - at the 5th day of proliferation and 24h and 72h after the initiation of differentiation.