DNA accessibility profiling at the single nucleus level of post mortem white matter brain tissue of patients with progressive multiple sclerosis and non-neurological disease controls
Different lesion types were microdissected out from snap-frozen white matter. Brain nuclei were fluorescence-activated nuclear sorted and treated with Transposase Tn5. Gel beads-in-emulsion was generated, barcoded  followed by library construction and sequencing. Overall design: snATAC-Seq