ChIP-seq identification of CarD (MSMEG_6077) binding sites in Mycobacterium smegmatis
CarD is essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with CarD-FLAG (LK2539), where an additional copy of CarD under anhydrotetracycline inducible promoter was stably inserted into the genome. CarD-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.