scCNA-seq
To determine whether CD45+ CTCs originated from cancer, we sorted three populations of single cells from three colorectal cancer patients by FACS: (a) CD45+ Lineage-APC+ WBCs (Lineage contains CD3/14/16/19/20/56); (b) CD45+ Lineage-APC- EpCAM+ CTCs; (c) CD45- EpCAM+ CTCs. 65 cells (including 23 WBCs, 24 CD45+ CTCs and 18 CD45- CTCs) were performed single-cell CNA sequencing.