PGC-1a overexpression in SH-SY5Y cells
SH-SY5Y cells were transfected with either GFP control or PGC-1a overexpression adenovirus and RNA was isolated after 48 hours and sequenced in order to understand what genes are differentially expressed with the presence of PGC-1a in neuron like cells. This experiment shows that genes related to neuron health and mitochondiral function are controlled by PGC-1a. We also analyze the promoter of upregualted genes found here to figure out potential partners that co-regulate with PGC-1a. Overall design: Examination of gene expression resulting from PGC-1a expression in SH-SY5Y cells.