The influence on Muc2 positive cells and Gli1 positive cells after the knockout of Smad4 or Alk3 in Gli1 positive cell and the effect of IL1 or IL17 on colonic organoids
To investigate the influecne of BMP signaling on Gli1+ cells, RNA-seq were performed after Smad4 or Alk3 knockout. Next, RNA-seq of Muc2-mCherry cells were performed to reveal the regulation of Gli1+ cell on intestinal epithelium. Finally,  RNA-seq of colonic organoids were performed to reveal the regulation of IL-1 or IL-17 on epithelium. To investigate the influecne of BMP signaling on Gli1+ cells, RNA-seq were performed after Smad4 or Alk3 knockout. Next, RNA-seq of Muc2-mCherry cells were performed to reveal the regulation of Gli1+ cell on intestinal epithelium. Finally,  RNA-seq of colonic organoids were performed to reveal the regulation of IL-1 or IL-17 on epithelium. Overall design: One monthe after tamoxifen administration, FACS was performed to sort Muc2-mCherry or Gli1-tdTomato cells and then performed  RNA sequencing. Alao, RNA-seq were performed in colonic organoids at 12 or 72 hours after IL-1 or IL-17 treatments.