Potent HPIV3-neutralizing IGHV5-51 antibodies identified from multiple individuals show light chain and CDRH3 promiscuity
B cells specific to DNA-barcoded F glycoproteins of HPIV3 were sorted from human PBMC samples, Cells were distributed into microfluidic droplets containing unique cell-specific oligonucleotides using the 10X Genomics Chromium Controller. cDNA libraires from single cells were further amplified for BCR enrichment and DNA barcode enrichment (LIBRA-seq).