Raw data generated by next-generation sequencing
After screening mRNA-displayed peptide libraries and phage-displayed peptide libraries against the target proteins TROP2 and 4-1BB, the enriched phages were eluted and used to infect E. coli. Plasmid DNA was extracted from the E. coli, and the fragments of interest were amplified by PCR for sequencing. The samples were then submitted for high-throughput sequencing.