Characterization of Fungal Communities in Guanica State Forest Caves Using ITS Sequencing
16S rRNA gene amplicons sequences were analyzed across caves in the Guanica State Forest of Puerto Rico to investigate prokaryotic diversity and abundance. Samples were collected from nine caves: Cueva Carmen (CAR), Cueva Eugenio "No" Guerras (EUG), Cueva El Refugio (REF), Sumidero Lluveras (SUL), Cueva de los Murcielagos (MUR), Cueva Carita (CRT), Cueva Gena La Negra (GEN), Cueva Del Negro (NEG) and Lago de Guano (GUA). This study aims to enhance scientific understanding of microbial community composition and the contributions of prokaryotes to subterranean ecosystem dynamics, while supporting conservation efforts in these environments.