Plasmid RP4 coupled transfer system expose to PVC leachate for conjugative mechanism investigation
The samples in this experiment were derived from the conjugate cells of the PVC leachate treatment group, which were set up with three replicates in section 2.4.1. Each replicate was treated as an independent transcriptome sample for analysis. The RNA from the conjugation system was extracted using the RNA Pure Bacteria Kit (CWBIO, China) according to the manufacturer's instructions. The purity and integrity of the extracted RNA were assessed by agarose gel electrophoresis and analyzed with a spectrophotometer (Galloptech, China). The extracted RNA was transcribed into cDNA using reverse transcription polymerase chain reaction (RT-PCR) with a reverse transcription kit (Takara, Japan). The cDNA samples were then sent to Novogene (Beijing) for sequencing analysis using the PE150 strategy on the Illumina platform for high-throughput sequencing.