Gene expression profile at single cell level of human T cells stimulated via antibodies against the T Cell Receptor (TCR)
We used single cell RNA sequencing (scRNAseq) to compare gene expression in human T cells following stimulation with antibodies directed against a CD3 subunit (CD3e) versus the TCRß chain variable (V) domain Overall design: Purified human T cells from six healthy donors were stimulated with antibodies in vitro for 7 days and analyzed using scRNAseq