Identification of unique cell type responses in pancreatic islets to stress
We single cell sequenced multiplexed primary human islets treated ex vivo with ER and inflammatory chemicals, indexed cell type specific stress responses, found genes to regulate in SC-islets to reduce apoptosis. We conducted multiomic ATAC+RNA sequencing on human islets treated with BFA, DMSO, IL1B+IFNG+TNFa, or PBS Overall design: Single cell RNA sequencing of primary human islets treated with Brefeldin A, Thapsigargin, and a variety of cytokines