Transcriptome analysis of small intestinal epithelium from AIN-93G and CE-2-fed mice
The intestinal epithelium constructs complex and redundant barrier machinery to prevent the infiltration of foreign substances. Dietary pattern is a major factor that influences intestinal epithelial barrier functions. We have previously shown that a purified diet (AIN-93G)-fed mice exhibit reduced epithelial turnover, fucosylation, and antimicrobial peptide production in the small intestine, particularly in the ileum, indicating that a purified diet has a significant impact on the intestinal homeostasis. We performed an 3'mRNA-sequencing analysis to investigate the global changes in epithelial gene expression by purified diet feeding. RNA was extracted from isolated epithelial cells from the duodenum and ileum of AIN-93G-fed or a control diet (CE-2)-fed mice (n = 6 per group, 24 samples in total), and subjected to sequence using a synthesized cDNA library.