Plant DNA metabarcoding data of Lake Ulu
Here is the amplicon data of ancient plant DNA from a long sediment core of Lake Ulu (the Oymyakon region, eastern Siberia). PCR was performed with the “g” and “h” universal plant primers for the P6 loop region of the chloroplast trnL (UAA) intron (Taberlet et al., 2007). We produced three PCR replicates for sediment samples and DNA extraction controls and added a PCR negative template control (NTC) in each PCR replicate batch. Library preparation and next-generation sequencing (2×150 bp) on the Illumina NextSeq 500 platform were performed with two independent sequencing runs at Fasteris SA, Switzerland. The data from two runs were merged into a single dataset for the final interpretation.