Transcriptomic and Tfe3 ChIP-seq analysis of MIN6 cells upon KCl stimulation.
Pancreatic beta-cells of islets secrete insulin in response to a rise in plasma glucose levels. However, the ability of beta-cells to secrete insulin decreases during progression of diabetes. Effect of insulin secretion on beta-cell function and its transcriptional program, if any, is not well understood. To determine changes in transcriptome in response to acute insulin secretion from beta-cells, an insulinoma-derived cell line MIN6 was stimulated with KCl, which induce membrane depolarization and subsequent insulin secretion, and an RNA-seq experiment was performed. In addition, to explore roles of stress-responsive transcription factor Tfe3 in the transcriptomic changes in response to KCl stimulation, we performed ChIP-seq experiments of Tfe3.