Off-target effect of CWG-cPIP on gene expression of mouse hippocampus
CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in the brain, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-injected mouse hippocampus 3 weeks later, mixing with ERCC RNA spike-in controls. Overall design: Comparative gene expression profiling analysis of RNA sequencing data for the hippocampus of vehicle- or CWG-cPIP-treated C57BL/6J mice (n = 3 mice each).