SUMOylated Etv1 establishes tumor-initiating cells during mammary oncogenesis [scRNA-seq]
scRNA-seq was used to compare the cell populations and transcriptiome in 6-week-old and 10-week-old FVB, MMTV-NIC, MMTV-NIC-Ube2ifl/fl mice Overall design: Mammary glands were harvested from 6-week-old MMTV-NIC and MMTV-NIC/Ube2ifl/fl mice, and 10-week-old FVB, MMTV-NIC and MMTV-NIC/Ube2ifl/fl mice (all in FVB background).Freshly sorted basal and luminal epithelial cells were mixed at a 1:1 ratio for sequencing and analyzed using scRNA-seq