Transcriptomic analysis of wild-type, heterozygous mutant, and CRISPR knockout RB1 orbital adipose-derived mesenchymal stem cells differentiated into adipocytes under chronic insulin treatment to study gene dosage effects on insulin response
This study investigates the effect of RB1 gene dose on the transcriptional response of human orbital adipose-derived mesenchymal stem cells (AMSCs) to chronic insulin treatment during adipocyte differentiation. RNA sequencing was performed on wild-type, heterozygous RB1 mutant, and CRISPR RB1 knockout AMSCs to understand how RB1 dosage influences gene expression under prolonged insulin exposure.