Transcriptome comparison of tobacco cell cultures to address mechanism of auxin autonomous cell proliferation
The response to auxin is regulated by spatiotemporally specific expression of the auxin signalling machinery and by precise regulation of auxin concentration through multiple biosynthetic and metabolite pathways supported by auxin transport involving influx, efflux, and intracellular transport. The rapid and steady proliferation of cells in plant cell cultures presents unique model for studying mechanisms involved in cell cycle regulation. Here we present transcriptome analyses in the habituated auxin-autonomous BY-2H and VBI-2 cell lines. Overall design: To compare transcriptomic profiles of tobacco cell cultures, we isolated total RNA from cells harvested at the exponential (3 day old) and stationary (10 day old) stages of their growth cycle, three replicas for eas sample group.