Single Cell Transcriptomic Analysis of Lung and Hematopoietic Megakaryocytes from Embryonic and Adult Mice.
To create a transcriptomic library that spans multiple stages and sites of megakaryocyte development, scRNA-Seq was performed on cells isolated from embryonic day 13 fetal liver and lung, as well as week 10 adult bone marrow and lung. Enrichment of megakaryocytes in all tissues was accomplished using flow cytometry to sort for live-CD41+ cells. Only the top 1% of CD41+ events were captured in order to further enhance enrichment of megakaryocyte-lineage cells. Overall design: Single cell transcriptomic analysis of live-CD41+ megakaryocytes isolated from the murine fetal lung and fetal liver, as well as the murine adult lung and bone marrow.