Aldosterone stimulation of fibroblasts affects gene expression and function relevant to muscle injury and disease
Here, we report the RNA sequencing of fibroblasts isolated from wild-type (C57BL/10) neonatal hindlimb skeletal muscle. The fibroblasts were directly treated with the MR agonist aldosterone, the MR antagonist spironolactone, or vehicle control (DMSO). We find that aldosterone treatment increased expression of genes involved in fibrosis, muscle function, and metabolism, and that aldosterone treatment decreased expression of genes involved in cell migration and cytokine receptor binding. This data suggests that MR signaling can directly affect fibroblasts and contribute to pathology in skeletal muscle. Overall design: RNA-seq profiling of wild-type (C57/BL 10) neonatal mouse hindlimb skeletal muscle fibroblasts, treated for 48h with either 1 uM aldosterone, 1 uM spironolactone, or vehicle control (DMSO).