Ultrasensitive ribosome profiling of wild-type and Piwil1-deficient golden hamster MII oocytes
Ultrasensitive ribosome profiling (RIBO-lite) was performed on metaphase II (MII) oocytes from wild-type (WT) and Piwil1-deficient (Piwil1-/-) golden hamsters (Mesocricetus auratus). This dataset was generated to measure the translatome of MII oocytes and to determine whether PIWIL1 expression affects the translation of differentially expressed proteins (DEPs) during the mammalian oocyte-to-embryo transition (OET). By comparing these translatome profiles with corresponding single-cell mass spectrometry proteomic data, this study reveals that most DEPs in PIWIL1-knockout MII oocytes are regulated at the post-translational level rather than the translational level. These data provide valuable resources for understanding the post-transcriptional and post-translational regulatory functions of PIWIL1 in mammalian oocyte development and early embryogenesis.