Evaluating the effect of lyoprotectants in preserving community structure and bacterial viability in the lyophilized fecal community of pigs.
This study evaluated the effect of different cryoprotectants on bacterial cell viability and microbial community structure of fecal sample to be used for FMT after lyophilization. Different cryoprotectants were used to pre-treat fecal samples collected from pig before lyophilization. Some of the samples were then treated with propidium monoazide (PMA) before DNA extraction to enable DNA extraction from only viable bacterial cells.