The role of nucleosome dynamics during the monocyte to macrophage transition
We used MNase Transcription Start Site Sequence Capture (mTSS-seq) to map nucleosome distribution and sensitivity to MNase digestion over pol II promoters in THP-1 monocytes during phorbol 12-myristate 13-acetate (PMA) induced differentiation. We found that differentially expressed macrophage genes have stronger nucleosome positioning and greater occupancy prior to differentiation suggesting that these genes are poised for future expression. We found dynamic alterations in nucleosome sensitivity during early differentiation and the establishment of a new sensitivity profile in macrophages. We further observed that alterations in sensitivity corresponded to gains and losses of regulatory factor footprints within the promoter region. Overall design: Nucleosome mapping by micrococcal nuclease (MNase) digestion and DNA sequencing of promoter captured mononucleosomes from THP-1 monocytes before, during, and after PMA induced differentiation UPDATE: [09-30-2024] The bigWig files for GSM8037120-GSM8037135 were replaced. UPDATE: [10-08-2024] Additional bigwig files are included. The list of the additional files and their associated sample/raw files is included in the readme.txt.