Single cell sequencing of CD45+ stomach cells isolated from mice w/ or w/o H.felis infection
Single cell suspension of stomach cells was isolated from mice w/ or w/o H.felis infection and then flow-sorted to collect CD45+ immune cells. There were 2 libraries of uninfected mice and 3 libraries of H.felis infected mice, 6 mice in each library. Each cell suspension was subjected to 3' single-cell sequencing using 10x Chromium Controller with accompanying Chromium Next GEM Single Cell 3' Kit V.3.1. Libraries were sequenced on Illumina NovaSeq 6000 platform.