Sphingosine-1 phosphate receptor 1 (S1PR1) is crucial for maintaining the stem cell properties of acute myeloid leukemia stem cells
To identify the most important gene(s) for maintaining the stem cell properties of leukemia stem cells (LSCs), we analyzed the transcriptional profiles of LSC and non-LSC subsets from acute leukemia patients and identified S1PR1 as a novel biomarker of LSCs. Then to understand the underlying mechanisms of S1PR1-dependent stemness maintenance, we performed gene expression profiling analyses comparing CD34+S1PR1+ and CD34+S1PR1- cell fractions from AML patients and healthy donors; we also determined the global transcriptional response of KG1 cells to an inhibitor of S1PR1 (FTY720) at two concentrations. Overall design: Data set 1:   Three acute myeloid leukemia (AML) samples (patient #M1, #M2, and #M3) were sorted into paired LSC (CD34+CD38-) and non-LSC (CD34+CD38+, CD34-CD38+, and CD34-CD38-) fractions by flow cytometry. RNA-seq profiling was performed on the paired fractions for each individual.     Data set 2:  Three Philadelphia chromosome-positive B cell acute lymphoblastic leukemia (ALL) samples (patient #L1, #L2, and #L3) were sorted into paired LSC (CD34+CD38-CD58-) and non-LSC (CD34+CD38+CD58+, CD34+CD38+CD58-, and CD34+CD38-CD58+) fractions by flow cytometry. RNA-seq profiling was performed on the paired fractions for each individual.     Data set 3:  RNA-seq profiling was performed on CD34+S1PR1+ (labeled as P_pos) and CD34+S1PR1- (labeled as P_neg) bone marrow mononuclear cell fractions from AML patients (patient #9 and #10) purified by flow cytometry.     Data set 4:  RNA-seq profiling was performed on CD34+S1PR1+ (labeled as D_pos) and CD34+S1PR1- (labeled as D_neg) bone marrow mononuclear cell fractions from healthy donors (donor #1 and #2) purified by flow cytometry.     Data set 5:  RNA-seq profiling was performed on KG1 cells that were pre-treated with FTY720 at 3 µM (labeled as F3) or 4 µM (labeled as F4) for 24 h or sham treatment (labeled as F0) without FTY720.