Transcriptional profiling of EMB+ cells and EMB- cells across long bone and vertebrae
To compare gene expression profiling of EMB+ cells and EMB- cells whin CD45-Ter119-CD31--Thy1-6C3-CD200+CD105- population across long bone and vertebrae, we isolated related cells form long bone or vertebrae of zic1 -cre mtmg mice by FACS. Overall design: Comparative gene expression profiling analysis of RNA-seq data for (1)long bone zic1-cre negative CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB+, (2)long bone zic1-cre negative CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB-, (3) vertebrae zic1-cre negative CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB+, (4)vertebrae zic1-cre negative CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB-, (5) vertebrae zic1-cre positive CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB+, (6)vertebrae zic1-cre positive CD45-Ter119-CD31--Thy1-6C3-CD200+CD105-EMB-.  Each population has four replicates.