Optimized Protocol for Culturing Porcine Retinal Explants and RNA Isolation
The culture of porcine retinal explants serves as a valuable cellular model for studying retinal pathologies and their underlying molecular mechanisms. This protocol provides a detailed, step-by-step guide for dissecting the eye, isolating retina, and culturing primary porcine retinal explants for extended periods. Additionally, we outline procedures for RNA isolation and sequencing from cultured retinal explants, enabling molecular analysis of retinal biology. Overall design: RNA-seq proifle of organotypic culture of retina from sus scrofa for a dutation of 10 days