RNA-seq for hydroquinone indcued malignant transformaned TK6 cells.
The cells were collected, and the total RNA was extracted from the cell samples with TRIzol (Invitrogen, CA, USA) according to the manufacturers protocol. After assessing the quality and quantity of the RNA, first-strand cDNA was synthesized, followed by immediate second-strand cDNA synthesis. The purification of double-stranded cDNA was performed using clean DNA beads (VAHTS DNA Clean Beads, VAHTS, N411). Sequencing libraries were established using a VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (VAHTS, NR604) for Illumina Nova Seq (NEB, Ipswich, MA, USA). All of the libraries were sequenced as 2*150-bp paired-end reads.