Cut&Tag data analysis of TgSNF2L-mAID on the whole genome of Toxoplasma gondii tachyzoites [SNF2L Cut&Tag]
Transcriptomic analyses revealed the gene expression changes after SNF2L depletion. To see which of these differentially expressed genes are directly targeted by SNF2L, we used Cut&tag to assess the binding positions of SNF2L in the parasite's genome. Overall design: Cut&tag(Cleavage Under Targets and Tagmentation) for TgSNF2L-mAID in Toxoplasma tachyzoites. HA tag was expressed in fusion with SNF2L protein. The antibody used was Anti-HA tag (abcam,[12CA5] (ab1424)), lot number: 1054528-1.