Effects of overexpression of PTPN21 on gene expression of Jurkat cells
Acute Lymphoblastic Leukemia (ALL) is a malignant disease characterized by the abnormal growth of immature lymphocytes. The high resistance to chemotherapy drugs and frequent recurrence contribute to low long-term survival in ALL patients. Presently, PTPN21 is associated with cancer development. Our research revealed that elevated PTPN21 levels hindered the apoptosis of ALL cells triggered by chemotherapy drugs like vincristine and daunorubicin. Through a series of laboratory experiments, we established that PTPN21 regulates ALL drug resistance to vincristine via GADD45A and the JNK signaling pathway. Additionally, the over-expression of PTPN21 reversed the cell cycle arrest in the G2/M phase induced by vincristine. As a phosphatase, targeting PTPN21 inhibition may offer a new approach for addressing drug resistance in recurrent and refractory ALL in the future. Overall design: To investigate the key molecules involved in PTPN21-mediated apoptosis inhibition, we conducted transcriptome sequencing in Jurkat cells after 48h of incubation with VCR. Jurkat cells in both control and PTPN21 overexpressed groups were treated with VCR for 48 hours. Subsequently, cells from both groups were harvested for RNA sequencing (RNA-seq) with three replicates per group. The sequencing of Digital Gene Expression Tag Profiling and data analysis were outsourced to a commercial company (Pinggu Biotechnology Co., Wuhan, China).