Transcriptome-wide mapping of RNA:protein interactions of SRSF5 in HeLa cells
We performed SRSF5 iCLIP2 in HeLa cells expressing endogenously GFP-tagged SRSF5. Overall design: 5 biological replicates were subjected to the iCLIP2 procedure with UV-crosslinking at 254 nm. For IPs an anti-GFP antibody was used. No UV treatment served as negative control.