ChIP-Seq profiles of HIM-1, CSR-1, COH-3, H3K9me2 and CEC-5 at adult stage in C. elegans
Chromatin fractions of C. elegans were prepared from adult populations by formaldehyde cross-linking and sonication. The worm strain was any one of N2, him-8(tm611) IV, or met-2(ok2307) set-25(n5021) III. The C. elegans chromatins and the Drosophila chromatins were mixed at a DNA ratio of 100:1. The Drosophila chromatins served as a spike-in control. Chromatin immunoprecipitations were performed with antibodies against histone H2Av in Drosophila and simultaneously with antibodies against any of HIM-1, CSR-1, COH-3/4, histone H3K9me2, CEC-5 in C. elegans, or FLAG (mock). Libraries of DNA co-immunoprecipitated with the antibodies as well as DNA of input lysates were sequenced using Illumina HiSeq sequencer in paired-end read mode. The average fragment length for these libraries was 203 bp.