Early-life stromal niches orchestrate B lymphopoiesis at the brain's borders
Single cell RNA sequencing of murine brain border and bone marrow tissues at postnatal day (P)14 to profile early-life immune cells and their stromal/endothelial niches in these organs. Overall design: Dura mater, brain and attached leptomeninges/choroid plexus, calvarial bone marrow, and tibial bone marrow were extracted from three C57BL/6J mice at P14 for single cell RNA sequencing.