Single-nucleus multiome profiling of ventral hippocampus across female reproductive states
This study used single-nucleus multiome (RNA + ATAC) sequencing to profile transcriptional and chromatin accessibility changes in the mouse ventral hippocampus across the estrous cycle, sex differences, and peripartum states. C57BL/6J mice were used, and nuclei were isolated from ventral hippocampal tissue and processed using the 10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression platform. Overall design: Ventral hippocampal tissue from C57BL/6J mice was collected across distinct physiological conditions (proestrus, diestrus, male, pregnancy, postpartum). Single-nucleus multiome sequencing was performed to jointly measure gene expression and chromatin accessibility.