Identification of the IFN-stimulated genes in IFN-a2b-treated intrahepatic cholangiocarcinoma cells
Interferon-a2b (IFN-a2b) is a highly active cytokine protein that belongs to the interferon-a (IFN-a) family. As a pleiotropic cytokine, IFN-a2b has beneficial antiviral, antitumour, antiparasitic, and immunomodulatory activities. In vivo and in vitro, IFN-a2b can activate the JAK-STAT pathway (also known as IFN-I pathway) and function by inducing the transcription of downstream IFN-stimulated genes (ISGs).To identify the ISGs in intrahepatic cholangiocarcinoma (ICCA) cells and evaluate the therapeutic value of IFN-a2b, ICCA cells were treated with recombinant IFN-a2b protein. Total RNA samples were extracted and used for RNA-seq. Certain ISGs (e.g., MXs, IFITs, OASs) were found to be significantly upregulated upon IFN-a2b treatment. Overall design: This dataset included two groups of samples. Every group contained three repetitions (rep). IFN-a2b was dissolved in the double distilled water with 1% fetal bovine serum (FBS), which was recommended by the suppliers. HUCCT1 cells were treated by IFN-a2b (60 ng/mL) for 24h. Another group of HUCCT1 cells underwent the same treatment with the recommended solvent.  The total RNA was extracted and used for RNA-seq.