Murine conditional Cxadr (CAR) knockout using an Mx1-Cre system
In addition to a series of in vitro experiments characterizing the role of Coxsackie-Adenovirus Receptor (CAR) in erythropoiesis (GSE147963), in vivo proof of concecpt was obtained from an Mx1-Cre Cxadrfl/fl conditional knockout mouse model. After poly I:C injection (400µg on 3 consecutive days) to induce Cxadr deletion mice were observed for up to 10 months to study changes in erythropoiesis. After development of anemia, mice were sacrificed and myeloid BM progenitors sorted. RNA of purified populations was used for RNA-seq to study molecular effector mechanisms. Overall design: 4 mice per group (Mx1-Cre+ Cxadrfl/fl experimental mice vs. Mx1-Cre- Cxadrfl/fl controls); CMP, GMP, MEP and LSK progenitor populations were sorted for each mouse