Purification of mitoplasts from A549 cells for transcriptomic analyses
In addition to transcripts encoded by the mitochondrial genome, there are various transcripts encoded by the nuclear genome within mitochondria. To identify nuclear-encoded transcripts in A549 cells, we isolated mitoplasts and extracted RNA for transcriptome sequencing. Additionally, we investigated the effect of LRPPRC protein knockdown on nuclear-encoded mitochondrial transcripts.