Next Generation Sequencing Facilitates Quantitative Analysis of EL4+Ctrl and EL4+RGFP966  cells' Transcriptomes in T cells  lymphoma
We here reported that HDAC3 inhibition induces the infiltration of NK cells in lymphoma and enhances their cytotoxicity by promoting the secretion of CXCL12 from lymphoma cells. RNA-seq analysis of EL4 cells treated with or without RGFP966 showed that chemokine signaling was significantly enriched in the HDAC3 inhibitor-treated group. And the expression of CXCL12 in mRNA and protein level were increased. ATF3 overexpression decreased the mRNA level of CXCL12 in lymphoma cells and reduced CXCL12 transcription can be reversed by HDAC3 inhibition in H9 cells. To sum up, targeting HDAC3 promoted the secretion of CXCL12 by inhibiting ATF3's transcriptional activity. Overall design: to analysis the differiational genes and pathways in EL4+Ctrl and EL4+RGFP966 lymphoma cells,we treated cells with vehicle or HDAC3 inhibitors (RGFP966) we then performed gene expression profiling analysis use data obtained from RNA-seq of EL4 cells treated with vehicle or RGFP966