Define the genomic targets of C. elegans MYRF-1
C. elegans myrf-1 gene encodes an essential homotrimeric transcription factor that accumulates rhythmically, once per larval stage, in all somatic tissues. Similar to its mammalian ortholog, MYRF-1 is synthesized as a full-length membrane-associated precursor that undergoes self-cleavage to release an N-terminal nuclear domain (MYRF-1(ND)). To define the genomic targets of MYRF-1, we performed ChIP-seq on staged animals expressing an endogenously tagged GFP-MYRF-1 fusion at peak nuclear abundance during the L1 stage.