The m6A reader HNRNPC promotes non-small cell lung cancer progression by enhancing E2F2 mRNA stability
HNRNPC, as one of the m6A reading proteins, has been confirmed to be highly expressed in a variety of tumors and promote the occurrence and progression of tumors. Previous studies have confirmed that HNRNPC significantly promoted the proliferation, migration and invasion of NSCLC cells, and its high expression was related to the clinical stage, lymph node and distant metastasis of patients, and could be used as an indicator of poor prognosis of NSCLC. However, the role of HNRNPC in regulating gene expression through m6A methylation modification in the occurance and progression of NSCLC remains largely unexplored. To investigate the target genes bound by HNRNPC, samples from A549 cells were harvested by RIP lysis buffer, and then the lysates were immunoprecipitated by 5µg anti-HNRNPC antibody. finally, the potential target genes were identified by RIP-seq. Overall design: A549 cells were harvested by RIP lysis buffer, and then the lysates were immunoprecipitated by 5µg anti-HNRNPC antibody. The purified input and HNRNPC-IP RNA samples were then subjected to RNA-seq analysis.