mNET-seq on INTS11-degron cells
We measured the the dwell time of existed paused RNAPII at the promoter by mammalian native elongating transcript sequencing (mNET-seq). Overall design: In order to examine and monitor the effects of INTS11 on the temporal stability of promoter RNAPII, we performed the mammalian native elongating transcript sequencing (mNET-seq) on INTS11-FKBP dTAG degron cells.