Effect of depletion of atp-2 by RNA interference on gene expression during the development of Caenorhabditis elegans
Disruption of physiological homeostasis elicits cellular stress response and behavioral changes of the animal. We recently showed that mitochondrial stress induces aversive associative memory for food bacteria that C. elegans inherently prefers without stress. Depletion of atp-2 by RNA interference in the non-neural tissues causes mitochondrial disruption and induces bacterial avoidance in C. elegans. Our transcriptome provide several metabolic pathways that may are critical for aversive memory.  Among these pathwaies, we found that peroxisomal ß-oxidation mediates serotonergic neuron,NSM, to promote stress-induced aversive memory. Overall design: To dissect the potentail pathways that drive aversive memory, We harvested the control worms and worms under atp-2 depletion by RNAi We then performed gene expression profilling using the data from RNA sequencing of control and atp-2 RNAi group We then performed Gene ontology analysis of the RNA sequencing data and found the pathways which may be critical for stress-induced aversive memory