Transcriptome expression changes after knockdown of ALDH1A1 in human colon cancer cells HT29
Firstly, shRNA (GCCAAATCATTCCTTGGAATT) was used to knock down ALDH1A1 in HT29 cells, and the knockdown effect was verified by western blot. Then the shALDH1A1 HT29 cells were compared with parental HT29 cells by RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for shALDH1A1 HT29 cells and parental HT29 cells.