CBFB-DSP-ChIPseq and YAP-DSP-ChIPseq
We developed a new method to extract  open-chromatins  that contain trans-acting factors (TAFs)-DNA complex crosslinked with dithiobis(succinimidyl propionate) (DSP). Using this method we identified CBFB as a cell density-sensitive TAF, which translocates between the cytoplasm and the nucleus in cell density-dependent manner.  Here, we demonstrate that the extracted TAF/DNA complex is also amenable to ChIP-seq analysis. Overall design: Genome wide examination of CBFB binding region in human skeleton muscle cells.