Caecal adenoma single cell mRNA sequencing from Apcfl/flSmad4fl/flLgr5CreERT2 and Apcfl/flSmad4+/+Lgr5CreERT2
Pooled single-cell suspensions from were obtained from caecal tumours from three Apcfl/fl,Smad4+/+, Lgr5CreERT2 and five Apcfl/fl, Smad4fl/fl, Lgr5CreERT2  mice.  Cell suspensions underwent library preparation using the Chromium Single Cell 3' kit (10X Genomics) and sequencing using Illumina HiSeq 2x150bp (Azenta, USA), with approximately 25,000 reads per cell.