The genes involved in histone deacetylation regulates lipid metabolism through H4K5 and H4K8
ChIP-seq and motif analysis of C. elegans which were fed with E. coli OP50 until reach to adult. After washing by M9 buffer for three times, worms were re-suspended in PBS, frozen immediately in liquid nitrogen for ChIP-seq which were carried out by Wuhan SeqHealth Tech Co.,Ltd (Wuhan, China). The chromatin was sheared into 0.2 to 1kb fragments then subjected to immunoprecipitation using antibodies for H4K5ac (Abcam, ab51997) or H4K8ac (Abcam ab45166). Indexed ChIP-seq libraries were prepared using the NEB Next Ultra DNA library prep kit (New England Biolabs) and pair-end sequences on iIIumina NovaSeq 6000. HOMER was used in motif analysis of deferentially enriched peaks 31.