Integrative RNA-seq and CLIP-seq analysis reveals hnRNP-F regulation of the TNFa/NF?B signaling in high glucose conditions
Using RNA-seq and ChIP-seq we found that we found that hnRNP-F may bind to lncRNA SNHG1 to negatively regulate the transcription of genes involved in the TNFa/NF?B signaling pathway in diabetic nephropathy. Our study suggests that hnRNP-F may play a role in diabetic nephropathy by regulating the differential expression and variable splicing of diabetic nephropathy-associated genes, especially those related to inflammatory response. Overall design: In this study, hnRNP-F was overexpressed in human renal proximal tubular epithelial (HK-2) cells cultured in high glucose conditions, while an empty vector was transfected into HK-2 cells as a control group (NC). RNA-seq was utilized to generate transcriptome data following hnRNP-F overexpression, allowing for the analysis of differential gene expression and alternative splicing events influenced by hnRNP-F overexpression. We also downloaded the CLIP-seq data of hnRNP-F in human 293T cells from GEO database. Through integrative analysis of RNA-seq and CLIP-seq, we tried to identified a set of potential direct targets of hnRNP-F in cells.