Cell-type-specific contributions to impaired blood-brain barrier and altered cerebral metabolism in prodromal 5XFAD mice
Single-nucleus RNA sequencing (snRNA-Seq) further revealed cell type-specific transcriptomic changes in the hippocampus of prodromal 5XFAD mice, including downregulation of synaptogenesis and synaptic transmission genes in the CA1 and dentate gyrus excitatory neurons, impaired endothelia gene expression linked to brain barrier function and angiogenesis, altered innate immune response genes in astrocytes, as well as upregulation of cholesterol biosynthesis and metabolism genes in the CA1 excitatory neurons. Overall design: We performed snRNA-seq in mouse hipppcampus from 3 months old 5xFAD and wild type mice with and without blood vessel enrichment.