Expression profiling in exosomal miRNAs of health individuals and newly diagnosed multiple myeloma
Multiple myeloma (MM) is a hematopoietic malignancy.Based on its laboratory and clinical presentation it can be summarized as MGUS, smoldering myeloma, and multiple myeloma. Previous studies have shown that the expression levels of miRNAs in different stages of the disease are different and exosomes are involved in modulating the progression and the metastasis of cancers through miRNAs. This study analyzes the expression levels of miRNAs in healthy individuals and myeloma bone marrow serum exosomes. Overall design: BM samples were obtained from 4 MM patients and 2 healthy individuals .The exosomes from bone marrow were isolated by ultracentrifugation. Exosomal miRNA profiling was sequencing on Illumina NextSeq 500.