RNA-seq data for characterization of wooden breast myopathy in chicken
Breast muscle (Pectoralis major) samples (n=60) were collected from 36 days post-hatching male Ross 308 broiler chickens (Gallus Gallus) which were fed by a pelleted wheat/maize diet ad libitum from 10 days of age. Chickens were in 2.4 x 0.95 m pens on wood shavings in a room with 6 h light and 18 h darkness and a temperature gradually reduced from 28°C to 21°C. Samples for qPCR, RNA-seq, proteomics, western blotting, and zymography were excised immediately after slaughter, snap-frozen in liquid nitrogen and stored at -80 °C until further analysis. All animals were first sorted by palpation, then classified by histology, and near infra-red (NIR) spectroscopy.