Effects of the Sox17mut5 mutation on SOX17 function in mouse embryonic gallbladder development
To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17mut5 mutant animals and surveyed how this mutation altered Sox17 expression.  Gallbladders were isolated from P21 Sox17mut5/mut5 and Sox17+/+ (n = 4 of each genotype), RNA  isolated, and bulk RNA-Seq performed.