Transcriptome sequencing of rice root tissue
Research Objective: This study aims to investigate the function of the OsDD8 gene and the OsDD2/3/6/8 gene cluster in rice. Specifically, the goal is to elucidate their mechanistic role in root system development.Study Design: We performed transcriptome sequencing (RNA-seq) on root tissue samples from the following rice lines: wild-type, the Osdd8 single mutant, and the Osdd2/3/6/8 quadruple mutant. Through comparative analysis of the gene expression profiles from these samples, we aim to identify downstream target genes regulated by OsDD8 and the OsDD2/3/6/8 complex.Scientific Significance: This research will reveal the critical function of OsDD8 and OsDD2/3/6/8 in regulating the development of root cortical cell architecture in rice. The findings will not only provide new insights into the molecular regulatory mechanisms in rice but also offer important theoretical foundations and genetic resources for future molecular breeding efforts aimed at improving rice stress tolerance and overall crop resilience.