Effect of GSK3 inhibition on the transcriptional signature of human monocytes
We explored the molecular impact of blocking GSK3 on the gene expression profile in human monocytes. GSK3 inhibition by CHIR-99021 redirected monocytes towards acquiring an anti-inflammatory gene profile similar to M-CSF-primed monocyte-derived macrophages. Overall design: mRNA profiles of purified human monocytes treated DMSO or 10 micromolar of GSK3a/ß inhibitor (CHIR99021) or 10 ng/mL M-CSF for 16 hours.