Multicompartment microbiome restructuring in rice under Fusarium infection and synthetic microbial consortia
This project investigates the restructuring of bacterial communities across soil, rhizosphere root, and leaf compartments of Oryza sativa under Fusarium infection and microbial inoculation. The experimental design includes five treatments: healthy control (CK), Fusarium infected control (CK+F), single strain biocontrol (Bacillus amyloliquefaciens FH1), and two synthetic microbial consortia (SMC3 and SMC5) differing in functional composition (heterotroph dominant vs phototroph enriched). Total DNA was extracted from soil, root, and leaf samples. Bacterial communities were characterized by amplification of the V3-V4 region of the 16S rRNA gene using primers 338F and 806R. Fungal communities were characterized by amplification of the ITS2 region using primers ITS1F and ITS2R. Amplicon libraries were sequenced on an Illumina platform using paired-end reads. The dataset includes replicated samples across all treatments and compartments, enabling comparative analysis of microbiome assembly under pathogen pressure and microbial intervention. The study aims to identify microbial signatures associated with disease suppression, evaluate the contribution of synthetic consortia to microbiome stability, and assess how phototrophic components influence microbial community structure and function in the plant soil continuum.