RNA-seq of laser-capture microdissected tissues from Lupinus angustifolius cv. Oskar leaf, stem, and pod organs
Tissues were collected from cryosections using laser-capture microdissection. In general, epidermis, chloroplastic parenchyma, and vascular tissues were collected from leaf, stem, and developing pods at 30 days after anthesis to identify tissue-specific expression of genes. Libraries were prepared from 10 ng total RNA for each sample, using the SMART-Seq Ultra Low Input RNA kit and sequenced on an Illumina platform (HiSeq X) to generate 151-bp paired-end reads.