Sequencing of mouse endothelial progenitor cells treated with VUF11207
RNA was extracted from mouse endothelial progenitor cells (EPCs) cultured under standard conditions (CTRL group) and from those treated with VUF11207 (VUF11207 group). Library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) with 1 ug of RNA per sample. Sequencing was carried out on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA), generating 150-bp paired-end reads. The quality of the raw sequencing data was evaluated using FastQC (v0.11.9). Alignment of reads to the GRCh38 human reference genome was performed using HISAT2 (v2.2.1). Gene expression quantification was achieved with FeatureCounts (v2.0.1) and normalized to counts per million (CPM). Differential gene expression analysis was conducted using DESeq2 (v1.20.0), and the Benjamini-Hochberg method was applied to control the false discovery rate (FDR). This study aims to identify transcriptional changes in EPCs induced by VUF11207, providing insights into CXCR7-related functions in endothelial biology.