ccRNA identification by circularization and miSeq
Efforts to identify ccRNA by sequencing by gibson circularization after template-switching 5' RACE using a cRNA-end specific primer and subsequent sequencing by Illumina miSeq. Sequencing of wild-type A/WSN/1933 and a variant bearing the mutation T677A in the PB1 subunit. Overall design: A549 cells were infected with the indicated variants at an MOI of 1, RNA harvested 16hpi, and samples processed using a custom sequencing pipeline.