iPS2-seq clonal drift on hiPSC after transfection from early passage (P3) to 10 additional pasages (P13)
We tested two batches of transfection with the iPS2-seq pool plasmid library. iPS2seq allows the identification of transfected clones with a dual barcoding technology: a UCI or clonal identifier is a barcode generated during the plasmid preparation and identifies each plasmid in a single way, then a shRNA barcode identifying the targeting shRNA. We tested for clonal drifting after culture of the transfected cells from early passage (passage 3) and then after 5 and 10 additional passages. We performed a PCR to amplify the targeting region and added indexing adapters to build a library, according to the cell QC protocol provided with the publication.