RNA-Seq of mRNA derived from fourth larval stage larvae from C. elegans strain PE1328 treated for 16 hours at 20 degrees Celsius with 5-Ph-IAA compared to untreated controls
The objective of the experiment was to determine the impact of cap-adjacent 2'-O-ribose methylation on steady-state mRNA levels in a background lacking the decapping exonculease EOL-1. We depleted CMTR-1 protein, which is the main enzyme responsible for of cap-adjacent 2'-O-ribose methylation in C. elegans. This was achieved using a strain that is homozygous for an auxin-degron allele of the endogenous cmtr-1 gene in genetic background that constitutively expressed TIR1(F74G) protein (cshIs140 allele derived from HML1012), and also for an eol-1(fe172[E197K]) mutation. The depletion was achieved using the auxin analogue 5-Ph-IAA. Experimental data consists of six replicates, and depletion of CMTR-1 was confirmed by Western blotting.