Detect IGF1R function in postnatal bone marrow mesenchymal stem cell
1. IGF1R signaling pathway is highly enriched in P0.5 BMMSC compared to adult BMMSC; 2. Niche factors are highly expressed in PDGFRa+Sca1+ (PaS) cells. 3. Knockout IGF1R in Prx1+ BMMSC significantly impaired niche factors expression in P0.5 BMMSC. Overall design: Flow sorted adult PDGFRa+ adult BMSCs, perinatal PDGFRa+Sca1+ BMSCs, perinatal PDGFRa+Sca1- cells, and PDGFRa+Sca1+ perinatal BMSCs with or without IGF1R deletion for RNAseq.