Effect of Tigit deficiency on CD4 T cell
To  investigate the role of TIGIT in CD4+ T cell differentiation, Total CD4+ T cells were isolated from WT or Tigit -/- mice. Cells were stimulated with anti-CD3/CD28 for 3 d. Cells were then collected and total RNA was extracted. Overall design: Total CD4+ T cells were isolated from WT or Tigit -/- mice. There were 3 biological replicates in each group.