P. knowlesi mRNA data of 5 blood stage time points
This study provides P. knowlesi line A1-H.1 bulk mRNAseq data at five time points in the inthraerythrocytic developmental cycle (IDC): 5 hours post-invasion (hpi) rings, 14 hpi mid-trophozoites, 20 hpi late trophozoites, 24 hpi mid-schizonts and 27 hpi late schizonts.  The parasite culture was tightly synchronized within a 1-hour window, and harvested at the above mentioned time points. mRNA library preparation was carried out with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, and human globin mRNA was blocked using the QIAseq FastSelect - Globin Kit.  In addition, P. knowlesi line A1-H.1 was whole genome sequenced as part of the same experiment in which RNA samples for transcriptional analysis were collected.  Library preparation was carried out by Novogene (Munich, Germany) using their PCR-based 'Microbial WGS' workflow (including 10 cycles of PCR amplification).  Preprint available at bioRxiv: https://www.biorxiv.org/content/10.64898/2026.02.09.704036v1