Polydatin-Curcumin Formulation Alleviates CTD-ILD-like Lung Injury in Mice
All mice were acclimatized and fed for 1 week prior to formal experiments. Referring to the relevant literature for model preparation, mice were anesthetized, and 50 µL of bleomycin (1 mg·mL-1, dissolved in PBS) was rapidly dripped into the trachea, rotating the body so that the drug diffused to the whole lung; normal controls were given 50 µL of PBS by tracheal drip in the same way as above. One week after modeling, the bleomycin–treating model was formed. Subsequently, pharmacological interventions were administered using intraperitoneal injections (i.p.) after randomization into groups. The PD+Cur group received a mixed solution of PD and Cur (1:1). The normal and model groups received the same volume of PBS, i.p. All mice were injected in a volume of 20 ml·kg-1 every other day for 4 weeks.