Transcriptomic analysis of AASS-knockdown H1299 cells to identify its role in metastatic potential of lung adenocarcinoma
Through a metabolic RNAi-based screen for cell invasion, potential metastatic functions of AASS were identified. This study was to investigate the role of AASS and its metabolic product, a-aminoadipate (2-AAA), in the metastatic potential of lung adenocarcinoma. H1299 lung adenocarcinoma cells were infected with a pLKO.1-based lentiviral vector carrying shRNA targeting AASS. Poly-A RNA sequencing was then performed on control and AASS-knockdown H1299 cells to assess differentially expressed genes modulated by AASS depletion.