Gene expression profile at single cell level of immune cells from STING-NP treated 4T1 BALB/c tumors
scRNA-seq was performed on immune cells isolated from 4T1 BALB/c tumors to investigate their response to various treatments. Overall design: Cells from tumor suspension were washed and labeled with anti-CD45 antibodies (to label immune cells) and Total-seq anti-mouse Hashtag antibodies (for multiplexing) and sorted prior to scRNA-seq