Homo sapiens Raw sequence reads
To elucidate the transcriptomic mechanisms underlying the antitumor activity of SJ25, we performed RNA sequencing on colorectal cancer (CRC) cells HCT16 treated with SJ25 or vehicle control. This analysis aimed to:Identify differentially expressed genes (DEGs) and enriched pathways modulated by SJ25, particularly focusing on its dual inhibition of the EGFR/Raf/MEK/ERK pathway.