Whole genome sequencing of CISH-disrupted CAR-NK cells and control NK cells for off-target analysis
This project investigates genome-wide off-target effects of CRISPR/Cas9-mediated CISH disruption in chimeric antigen receptor (CAR)-engineered natural killer (NK) cells. Whole genome sequencing (WGS) was conducted on CISH-disrupted CAR-NK cells and corresponding unedited NK cell controls to identify potential unintended genomic alterations and evaluate genomic integrity.