Seed endophytic bacteria shift across lima bean generations
This study investigated how the seed-associated bacterial communities change across successive generations of lima bean (Phaseolus lunatus L.) during plant breeding. Using 16S rRNA gene sequencing, we analyzed the seed endophytic microbiome from parental lines through advanced generations (F2 to F9). The results revealed a progressive restructuring of the seed bacteriome, with specific bacterial groups, such as Pseudomonas, Burkholderia, and Lysinibacillus, becoming enriched in later generations, while early lines were dominated by Methylobacterium and Anaerobacillus. These findings indicate that host genotype and generational advancement influence the inheritance and composition of seed microbiota, highlighting the role of microbial selection during crop improvement.