The effect of Histone H3.3 knockdown on DNA methylation in HepG2 cells
Whole genome bisulfite sequencing (WGBS) is performed to study the effect of H3.3 on DNA methylation in HepG2 cells. Overall design: The expression of Histone H3.3 was knocked down by lentiviruses-mediated shRNA in HepG2. The control and H3.3-deficient HepG2 cells were subjected to WGBS and genome-wide DNA methylation analyzing.