IKAROS re-expression modulates transcriptional networks in IKZF1-mutant Ph+ B-ALL [RNA-Seq]
With the goal to investigate tumor suppressor mechanisms regulated by IKAROS (IKZF1) in PH chromosome–positive B-cell acute lymphoblastic leukemia (B-ALL) harboring IKZF1 mutations. This dataset includes bulk RNA-seq profiles from human MXP5 and PDX2 cell lines, which were engineered to express doxycycline-inducible wild-type IKAROS (IK1) or an empty vector control. RNA was collected 24 hours post-induction to capture early transcriptional responses to IK1 re-expression. These data are part of a larger multi-omics study integrating RNA-seq, ChIP-seq, CUT&RUN, ATAC-seq, and HiChIP to define IKAROS-regulated transcriptional and chromatin networks in IKZF1-deficient Ph? B-ALL. Overall design: The experimental design includes two IKZF1-deficient Ph? B-ALL cell lines (MXP5 and PDX2) expressing either doxycycline-inducible wild-type IKAROS (IK1) or empty control vectors. Cells were treated with 1 µg/mL doxycycline for 24 hours prior to RNA extraction. Each condition was performed in biological duplicate, resulting in eight total RNA-seq libraries. This design supports comparisons between IK1-induced and control samples within and across cell lines to identify IKAROS-regulated transcriptional programs. *************************************************************** The table below lists GEO accessions reused/reanalyzed for this study. ***************************************************************