Metagenomic sequencing of diversity outbred mice on dietary restriction
We performed longitudinal metagenomic sequencing of the gastrointestinal microbiome from more than 900 genetically diverse, female mice undergoing dietary restriction. The genetically diverse mice were Diversity Outbred mice from generations 22-24, 26-28. The dietary restriction regimens included ad libitum feeding (control group), fasting one day per week, fasting two consecutive days per week, 20% caloric restriction, and 40% caloric restriction.