RNA-seq analysis of HEK cells transfected with Capsid Anchor pGFP vs pEGFPNI vector
Whole-transcriptome sequencing was performed on four samples. High-quality paired-end reads (6.64–9.70 Gb per sample) were generated on the Illumina platform using 2 × 150 bp chemistry. The reads were aligned to the Homo sapiens reference genome (GRCh38) using STAR (v2.7.10a) with default parameters. Mapping rates ranged from 91.09% (pEGFPNI vector-transfected HEK cells, replicate 2) to 94.18% (Capsid Anchor pGFP-transfected cells, replicate 1). Differential expression analysis was carried out using the DESeq2 R package.