donkey and horse Genome sequencing and assembly
In this project, we aimed to achieve telomere - to - telomere (T2T) level genome assemblies for both horse and donkey.Blood tissues were carefully collected from representative horse and donkey specimens. For genome assembly, we utilized a comprehensive set of sequencing data. PacBio HiFi data, renowned for its high base - calling accuracy, was used. Additionally, we incorporated Oxford Nanopore Technologies (ONT) data with an N50 greater than 100k and ultra - long ONT data with an N50 greater than 200k. Hi - C data, essential for scaffolding and determining chromosomal structure, was also included.For the horse, our objective was to assemble its 62 autosomes and the XY sex chromosomes. Regarding the donkey, we aimed to assemble its 60 autosomes and the XY sex chromosomes.To enhance the assembly quality further, we used next - generation sequencing (NGS) short - read data to fill gaps in the draft genomes. Moreover, chromatin immunoprecipitation sequencing (ChIP - seq) data was applied to accurately identify centromeric regions, which are crucial elements in chromosomal organization.Through these integrated and systematic methods, we successfully completed the T2T - level genome assemblies for both horse and donkey. These high - quality genomic resources will facilitate in - depth research in equine genetics, evolution, and other related fields.