Pseudomonas exacerbates the apple replant disease induced by Fusarium
In this study, rhizosphere soils from apple trees continuously cropped were employed as materials, and the occurrence mechanism of Apple Replant Disease (ARD) was revealed from the microbiome through high-throughput sequencing, co-occurrence network, NetShift and correlation analysis. Core microbes were isolated, and their roles were verified through reinoculation experiments. The results indicated that the rhizosphere soil bacterial diversity of apples decreased and the fungal diversity increased, and the rhizosphere microbial network transformed from a "bacterial type" to a "fungal type". Pseudomonas might be a key soil genus in the occurrence of ARD, correlation analysis revealed for the first time that Pseudomonas was negatively correlated with bacterial diversity but positively correlated with Fusarium. Reinoculation experiments verified that adding key Pseudomonas ASVs indeed exacerbated the occurrence of replant diseases in strawberry and apple. The findings of this study provide new insights into the mechanism underlying the occurrence of ARD.