Luteolin inhibits Th17 cell differentiation and spinal infiltration in RA [RNA-seq]
RNA-seq was used to explore the DEGs from control vs. CIA and CIA+LUT vs. CIA CD4+ T cell Overall design: Total RNA was extracted from CD4+ T cells by using TRIzol Reagent (Invitrogen, Cat NO. 15596026). The RNA purity, concentration, and integrity were evaluated with an Agilent TapeStation 4200 (Agilent Technologies). RNA sequencing library was prepared by mRNA Library Prep Kit for Illumina®, and the indexed libraries were subjected to sequencing on DNBSEQ-T7 sequencer with PE150 model. Read mapping was performed via STRA software (version 2.5.3a) using CHOK1GS from Ensembl as a reference genome. Afterwards, high-quality reads mapped to the exon regions of each gene were counted by featureCounts (Subread-1.5.1; Bioconductor) and then RPKMs were calculated. Differential expression analysis was identified using the edgeR package (version 3.12.1).