Rheumatoid Arthritis circulating CD14+ monocytes are epigenetically primed [ChIP-seq]
Peripheral blood samples were collected from healthy controls and rheumatoid arthritis patients (active disease: DAS28 > 2.7), subsequently CD14+ monocytes were isolated using a positive selection kit, ChIP-Seq was completed for H3K4me3 and H3K27me3. Overall design: ChIP-Seq using the Illumina NextSeq 500 was performed on circulating CD14+ monocytes isolated from 15 healthy controls and 10 rheumatoid arthritis patients.