The effects of broflanilide on developing zebrafish
For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis. Overall design: Exposure groups included 3 treatments:  DMSO control (n=5), 1 ug/L broflanilide (n=4), and 10 ug/L broflanilide (n=3).