Integration analysis of viral genome insertion in tumor tissues and adjacent tissues
Previous studies have demonstrated that rAAV vector integration into the Rian locus can lead to gene dysregulation, potentially contributing to tumorigenesis. To investigate whether similar integration events occur in our model, we examined hepatocellular tumors following rAAV treatment for evidence of viral integration. Through third-generation sequencing and comprehensive genomic analysis, we identified a potential tumor-associated integration event on chromosome 12 of the hepatocyte genome.