Raw scRNA-seq data for hepatic gamma delta T cells from No ABX, GF, and GFT mice (PRJCA038359)
The objective of performing scRNA-seq on hepatic ?d T cells in this study was to dissect the heterogeneity of these cells within the liver and investigate their interaction with the gut microbiota. Specifically, we aimed to characterize the transcriptional profiles of different ?d T cell subsets, focusing on V?4+ ?dT17 cells and their IL-17F+ subpopulation, in the context of microbiota-mediated regulation. This analysis will help us understand how the gut microbiota, via metabolites like indole-3-acetic acid (IAA), influences the gene expression programs of hepatic  V?4+ ?dT17 cells, including those driving their expansion and activation.