Whole-Genome Bisulfite Sequencing of Arabidopsis WT , cdk8-1 and ahl10 mutants under NaCl treatment
Genomic DNA from 10-day-old WT, ahl10, and cdk8-1 seedlings treated with or without NaCl for 5 h was extracted. 0.1 ug of genomic DNA and 0.5 ng of unmethylated lambda DNA were mixed and fragmented into 200-400 bp to generate Bisulfite Sequencing-seq (BS-seq) libraries. Libraries was pair-end sequenced on Illumina Novaseq platform (Illumina, CA, USA). BS-seq reads were mapped to the TAIR10 genome with BS-seeker.