Differential Expression Analysis of Serum Exosomal miRNAs in Patients with Colorectal Cancer Liver Metastasis
To establish the differential expression profile of serum exosomal miRNAs in patients with colorectal cancer liver metastasis. Colorectal cancer (CRC, C group) and colorectal cancer with liver metastasis (CRLM, L group) patients from November 2021 to November 2022 were selected as the case groups. A control group (Normal, N group) was randomly selected from individuals undergoing health examinations during the same period, matched for age and gender with the case groups. Illumina next-generation sequencing technology was employed to detect the expression of serum exosomal miRNAs, and the differential expression profile of serum exosomal miRNAs in CRLM patients was established. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses and Protein-Protein Interaction (PPI) network analysis was utilized to gain the relationships between the differentially expressed miRNA target genes.