The role of the transcriptional co-regulator Lmo4 in CD8+ T cell stemness, memory formation and antitumor immunity
We aimed to evaluate if Lmo4 is directly involved in the generation and preservation of stem cell-like T cells and if adoptively transferred Lmo4-overexpressing pmel-1 CD8+ T cells (which identify the shared melanoma-melanocyte differentiation antigen gp100) are therapeutically effective in mice bearing subcutaneous B16-hgp100 melanomas. Moreover, our purpose was to investigate the mechanisms by which Lmo4 regulates CD8+ T cell differentiation. Overall design: Gene expression was evaluated by RNA-seq of pmel-1 CD62L-KLRG1- T cells collected 5 d after transfer of 1 x 105 pmel-1 Thy1.1+ and Lmo4-Thy1.1+ CD8+ T cells into wild-type mice infected with gp100-vv (five mice per group) R package edgeR was used to identify differentially expressed genes