Whole-genome resequencing of F2 CSL_Chr4/5_C hybrids populations from Arabidopsis (Col x Ler, wild type and recq4ab figl1 mutants)
This experiment aims at analyzing crossover distribution in the Arabidopsis recq4ab figl1 compared to wild type. CSL_Chr4_C and CSL_Chr5_C populations were produced through successive crosses as: A first cross was done between recq4ab figl1+/- Col plants and parental homozygote CSLs with Chr4 and Chr5 pure Ler, respectively. The resulting plant was then backcrossed to the parental CSLs for Chr4 and Chr5. From the resultant BC1 plants, we selected the recq4ab figl1 +/- and we identified the lines with pure Ler Chr4 (or Chr5, respectively). The offspring from the selfing of the selected plant was crossed with Col recq4ab figl1 +/-. Among the resultant plants, we selected wild-type and recq4ab figl1 individuals as the parents of the populations used for recombination and QTL analyses. Leaf samples from the self-crossed population were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2 × 150 bp), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).