RAD-seq of 390 progeny from a cross of S. cerevisiae strains FY4 and S1278b as pilot for BEST genotyping and automated tetrad method.
RAD-seq reduced genome sequencing of 390 progeny from a cross of S. cerevisiae strains FY4 and S1278b, using restriction enzymes MfeI and MboI. Pilot study for BEST (Barcode Enabled Sequencing of Tetrads) where tetrad-specific plasmid barcoding allows reconstruction of tetrad relationships using the RAD-seq data.