This study explores the use of Spirulina spp. as a biofertilizer for Coriandrum sativum, focusing on its biostimulant effects. Soil samples treated with Spirulina biofertilizer were analyzed through metagenomics, targeting the bacterial community. 16S rRNA gene sequencing identified key bacterial species, with Phaselicystis flava and Woodsholea maritima being most abundant. Notably, Woodsholea maritima was found to promote plant growth. Other species like Brevundimonas vesicularis and Acinetobacter rhizosphaerae were also present. Beta diversity analysis revealed shifts in microbial community structure, supporting Spirulina biofertilizers potential as a sustainable, eco-friendly tool to enhance crop performance and productivity.
Spirulina, a genus of cyanobacteria, is gaining recognition as a promising biofertilizer due to its unique characteristics and potential biostimulant effects on plant growth. This study focuses on isolating, identifying, and producing a liquid biofertilizer from Spirulina spp. with the main objective of evaluating its biostimulant effect on Coriandrum sativum plants. To investigate the impact of Spirulina biofertilizer, soil samples associated with the coriander crop treated with the biofertilizer were analyzed. Genomic DNA extraction and library preparation were conducted for metagenomics analysis, specifically targeting the bacterial community within the soil microbiome. The resulting 16S rRNA gene sequencing data underwent quality control, Operational Taxonomic Unit classification, and beta diversity assessment to measure the variation in microbial community composition between treated and untreated soil samples. The findings revealed the presence of several bacterial species in the Spirulina biofertilizer treated soil sample, with Phaselicystis flavaand Woodsholea maritima being highly abundant, constituting 25 and 20 percentage of the total abundance, respectively. Other species such as Brevundimonas vesicularis, Hydrogenophaga palleronii, Lysobacter brunescens, Peredibacter starrii, Acinetobacter rhizosphaerae, Pseudoxanthomonas mexicana, and Terribacillus halophilus were also present, each accounting for less than 10 percentage of the total abundance. Understanding these changes is essential for optimizing the application of Spirulina biofertilizer and enhancing plant growth and productivity. Assessing the beta diversity of the soil microbiome provided insights into the potential shifts in microbial diversity and community structure associated with Spirulina biofertilizer application. The study contributes to the development of sustainable agricultural practices by highlighting the potential of Spirulina biofertilizer as an environmentally friendly approach to improve crop performance. The results reveals the presence of Woodsholea maritima promotes growth of plant.