Modulation of Integrin a9ß1 in Macrophages Reduces Periodontal Inflammation and Supports Alveolar Bone Preservation in Periodontitis [scRNA-seq]
Establish an experimental periodontitis model using Itga9-Lyz2-cKO mice and Itga9-Lyz2-cHet mice, and collect gingival tissues for single-cell analysis. Overall design: An experimental periodontitis model was established in transgenic mice by performing silk suture ligation and applying Porphyromonas gingivalis to the maxillary second molar. After 14 days, gingival tissues were harvested for single-cell sequencing.