Physiological Phenotype and Molecular Mechanism of Lysine Decarboxylase in Response to Salt Stress in Sophora alopecuroides
Lysine decarboxylase (SaLDC) is the first enzyme in the quinolizidine alkaloid biosynthesis pathway of Sophora alopecuroides L., catalyzing the decarboxylation of lysine to produce cadaverine. Our group previously identified SaLDC as a gene with upregulated expression under salt stress using multi-omics technologies. However, the biological function and molecular mechanisms of SaLDC under salt stress conditions remain unexplored.This project investigates the germination and seedling growth of Arabidopsis thaliana overexpressing SaLDC under salt stress conditions, aiming to elucidate the physiological role of SaLDC in salt stress tolerance. By combining transcriptomic and metabolomic analyses, we aim to identify key genes and metabolic pathways regulated by SaLDC that contribute to salt stress tolerance. The findings will not only shed light on the role of SaLDC in plant adaptation to saline environments but also provide a molecular foundation for understanding how Sophora alopecuroides maintains its medicinal quality under salt stress. This research has the potential to inform strategies for enhancing crop resilience to abiotic stress, offering broader applications in agricultural sustainability.