RNA sequencing data of primary cancer-associated fibroblasts (CAF) isolated from fresh ex vivo germ cell tumor (GCT) patient tissue
Single eng 3' RNA sequencing of primary CAF isolated from fresh ex vivo GCT patient tissue. Purity of CAF populations were verified by qPCR analysis of common CAF, GCT, endothelial and epithelial markers. 14 different CAF cultures from distinct GCT subtypes (seminoma(SE), non-seminoma(NS)) and 5 non-malignant fibroblast cultures were analyzed. Overall design: RNA sequencing of 6 SE-derived CAF (SE-CAF), 3 embryonal carcinoma-derived CAF (EC-CAF), 3 teratoma-derived CAF (TE-CAF), 2 Mix-CAF derived from mixed subtypes (SE and NS), and 5 non-malignant fibroblasts (nF).