Cardiac Thoracic DRG Poly RNAseq Data in rats post MI
The overall goal is to detect any signficant changes in the rat thoracic DRG mRNA expressions post myocardial infarction. Overall design: Thoracic DRGs were collected 8 weeks after MI or sham surgery (MI, n = 5; sham, n = 4). mRNA libraries were prepared on an Illumina NextSeq, generating 75bp reads. Demultiplexing used bcl2fastq v2.16.0.10. Adapters and low-quality bases were trimmed with cutadapt. Reads were aligned to the Rattus norvegicus rn6 reference with STAR v2.3.0. Gene-level counts were summarized against Partek v6.6 annotations (Partek Inc., St. Louis, MO, USA). Library sizes were normalized with the trimmed mean of M-values (TMM) method. Differential expression for MI versus SHAM was modeled in edgeR using a generalized linear model with likelihood-ratio tests and Benjamini-Hochberg FDR control.