RRBS of human fetal testis treated to a mixture of DEHP/BPA
Fetal human fetal testes (8-11 weeks post-fertilization [WPF]) were cut into small pieces (~1 mm3 ) and 4-6 pieces/testis (depending of the age or size of the gonad) were insert into the muscle of the left back of two twin female mice. One week after surgery, one mouse was exposed by drinking water to a mixture of BPA (1 µM, CAS no. 80-09-1, Merck) and DEHP (1 µM, CAS no. 117-81-7, Merck) and the other was exposed by drinking water to the vehicle (absolute ethanol [ETOH] diluted at 1/1000000 in water). Mice were exposed during 6-11 weeks (corresponding to the 18 WPF equivalent age) and at the end of exposure, testes were collected and each pieces were cleaned from extracellular matrix and murine muscular tissue. One part of the pieces were used for RRBS analyses.