Bacterial flora in malodorous cloths during washing process
These are metagenome data (16S rRNA gene V3-V4 region) in malodorous cloths and washed water. 20 malodorous cotton cloths in 3 household were used and bacterial 16S rRNA gene were extracted from these sample during washing process (washing, rinse, outdoor or indoor drying etc.) The goals of this project are to identify microflora that adhering to clothes and generation malodor in washed clothes. And, it aims to estimate where the bacteria come from and which washing process is effect of reducing microbial cells and malodors. Nucleotide sequencing was used next-generation Sequencing MiSeq (Illumina). Analysis of sequence data was used CLC Genomics Workbench, removing the low-quality part of sequencing read by Trimming, overlapping paired reads were combined by Merge, the read length was made constant by Length trimming, and OTU analysis was performed using Greengenes Databases.