Comparision of gene expression in human nucleus pulposus cells under hypoxia and normoxia condition
To investigate the effect of oxygen tension on gene expression of nucleus pulposus cells, human primary nucleus pulposus cells were cultured under condition with low(1%) or normal(21%) concentration of oxygen, respectively. Then, all cells were harvested, and total RNA was extracted using trizol prior to RNA sequencing. Overall design: Two biological replicates were performed under hypoxia and normoxia, total RNA extracted from normoxia-cultured nucleus pulposus cells were regarded as control