Single myonuclei RNA-seq on control vs AAV-OPDM mouse models
We injected in WT BL6 male mice either NaCl (control) or AAV.MYO4A coding either for the GFP (control) of for ATG polyG-GFP, OPDM2 uGIPpolyG-GFP, OPDM3 uN2CpolyG-GFP or OPML LOC6polyG-GFP. These diverse polyGlycine proteins are responsible oculopharyngodistal myopathy (OPDM). Our aim was to determine through single nuclei RNA seq whether expression of these polyGlycine-proteins in muscle leads to a common and specific signature. Overall design: Single nuclei RNA-seq analysis was performed using 10X Genomics FLEX multiplex technology which uses barcoded probe sets targeting the whole transcriptome and enabling sample multiplexing. Tibialis Anterior (TA), gastrocnemius and quadriceps muscles of 7 months post-AAV injection of two individuals per genotype : NaCl-, GFP-, ATG polyG-GFP-, uGIPpolyG-GFP-, uN2CpolyG-GFP- or LOC6polyG-GFP- injected mice.