RNA-Seq of Ribosomal prophiling in mice 2h post Hind-Paw Formalin injection
The goal was to identify actively translated sequences in Dorsal spinal cord from Mice 2hours after Hind-Paw 20% formalin injection. Ipsi and Contralateral sides were compared to identify transcriptional changes after acute pain. Tissue was collected and ribosomes were pulled-down in the presence of CHX, RNA was purified and sequenced afterwards.