Evolution of toxins in Nemertea through positive selection and gene duplication
The phylum Nemertea comprises venomous worms, most of which are marine and benthonic. Although the toxicity of these animals has been long known, systematic studies on characterization of toxins, mechanisms of toxicity and toxin evolution for this group are relatively scarce when compared to other venomous groups. Here we present a first investigation on the molecular evolution of toxins in Nemertea. Using a proteo-transcriptomic approach, we described toxins present in the body and poisonous mucus of the pilidiophoran Lineus sanguineus and the hoplonemertean Nemertopsis pamelaroeae. Using these new and publicly available transcriptomes, we investigated the molecular evolution of six selected toxin gene families. In addition, we have also characterized in silico the toxin genes found on the interstitial hoplonemertean, Ototyphlonemertes erneba, a first register on meiofaunal taxa. We identified 99 toxin transcripts in the pilidiophoran L. sanguineus, including previously known toxins, such as the alpha-nemertides and the Cytotoxins-A. However, for each of the hoplonemerteans, no more than 30 toxin transcripts were found. Occurrence of at least one gene duplication in each analyzed toxin gene was supported by genomic alignments and tree reconciliation methods. Evidence of positive selection was observed in all investigated toxin genes. Coupled to this, we hypothesize that an increased rate of gene duplications observed for Pilidiophora could be involved with the origin and expansion of toxin genes. Studies concerning the natural history of Nemertea are still needed to understand the evolution of their toxins. Nevertheless, our results show evolutionary mechanisms similar to those observed in other venomous groups.