Analysis of chromosomal structual aberratons in NB-4 cell line
This Hi-C experiment was a part of a research which analyzed chromosomal structural aberrations in hematological malignancies. First, the author conducted comprehensive detection of fusion transcripts in RNA-seq data of DSMZ's 99 cell lines, and then picked up cell lines with characteristic complex karyotype. NB-4 cell line is one of them, which shows characteristic fusion cluster in 8q24 region in the fusion detection step. This Hi-C experiment revealed amplifications of MYC, PVT1, and CCDC26 in 8q24 region. Additionally, several structural aberrations were found by scanning the whole genome. Overall design: in situ Hi-C of DSMZ's NB-4 cell line with no treatment