mesendoderm differentiation of WT and PHD2 knockout mESCs (scRNA-seq)
To study the cell composition and gene expression profile in the mesendoderm differentiation of WT and PHD2 knockout, we performed scRNA-seq on the cells collected from the differentiation of WT and PHD2 knockout AB2.2 mESCs at differentiation day 4. PHD2 knockoutout mESCs were constructed by CRISPR/Cas9. The differentiation was performed using a non-lineage tendency differentiation protocol. Overall design: scRNA-seq of WT and PHD2 knockout AB2.2 mESCs differentiated for 4 days