Characterisation of a pathogenic non-migratory fibroblast population in systemic sclerosis skin
Fibroblasts are central to pathogenesis of systemic sclerosis (SSc). However, studies of conventional explant fibroblast cultures incompletely reflect disease biology and treatment response. We isolated a second non-migratory “resident” population of fibroblasts from skin biopsies after outgrowth of explant “migratory” cells.  These non-motile resident fibroblasts were compared with migratory cells from the same biopsy, using functional studies, bulk and scRNAseq, and localised in situ by multichannel immunofluorescence.   Migratory and resident fibroblast populations in SSc showed distinct pro-fibrotic characteristics and gene expression for pathogenic pathways differing by stage and autoantibody subgroup.  TGFß signalling was highly active in migratory fibroblasts in early stage dcSSc.  Conversely, resident fibroblasts had less upregulated TGFß signalling, especially in late dcSSc.  Increased chemokine expression was a hallmark of resident fibroblasts at all stages.  In vitro studies confirmed differential response to TGFb1 and CCL2 between migratory and resident cells. We suggest that migratory fibroblasts are especially important in early skin disease whereas non-migratory fibroblasts may have a regulatory role and contribute more to fibrosis in later stage disease. Thus, we have identified a pathogenic fibroblast population in SSc, not isolated by conventional explant culture, that could play an important role in fibrosis and be targeted therapeutically. Overall design: Observational study comparing SSc and HC explant and resident fibroblasts in skin 4mm biopsies