The genome-wide targeting of flag (ETV4) in A549 cells
The ETS family of oncogenic TFs is emerging as crucial mediators of tumorigenesis in solid tumors. New insights into the molecular mechanisms hijacked by these factors will pave the way for novel therapeutic strategies. We have previously shown that ETV4 is the preponderant ETS factor that is associated with tumor progression and a worse prognosis in NSCLC. To determine the genome-wide chromatin binding of ETV4, we performed chromatin immunoprecipitation (ChIP)-sequencing studies in A549-shETV4 cells transfected with flag-ETV4 using flag antibody. In total, 54389 ETV4 binding peaks representing 25708 genes were identified (FC > 2), and 43.71% peaks were enriched in the promoter regions. Our ChIP-seq data showed that ETV4 is enriched in many genes that related with the wnt signaling pathway, MAPK signaling pathway, cell cycle, and autophagy, etc. Overall design: Human NSCLC cell line A549-shETV4 cells transfected with flag-ETV4, using anti-flag antibody..