Different expression of circulating microRNA profile in Tibetan OSAHS with Metabolic Syndrome patients
To identify distinctive miRNA signatures in OSAHS with Mets patients from healthy subjects, that could serve as diagnostic biomarkers or describe differential molecular mechanisms with potential therapeutic implications. 5 OSAHS with metabolic syndrome (MetS) patients and 4 Tibetan healthy subjects were selected as the control group from January 2018 to January 2021, who signed an informed, written consent form. All OSAHS Patients diagnosed by Polysomnography (PSG). Patients suffering from other respiratory diseases, or combined with allergic and autoimmune diseases, tumors and other serious systemic serious primary diseases were excluded from this study. Plasma was isolated and frozen at -80 ?C. Overall design: Four Tibetan healthy subjects and five Tibetan OSAHS patients with MetS were selected for high-throughput sequencing of miRNAs. Total plasma RNA was extracted with Trizol (Tiangen, Beijing) and assessed with Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and Qubit Fluorometer (Invitrogen). Total RNA per sample was used as input material for the small RNA library. Illumina Hiseq 2500 platform was used to sequence the library preparations and 50bp single-end reads were generated.