Effects of Oleanolic Acid on Gut Microbiota and Metabolic Homeostasis in High-Fat Diet-Fed Nile Tilapia (Oreochromis niloticus)
This study investigates the modulatory effects of oleanolic acid (OA) on the intestinal microbiota of Nile tilapia (Oreochromis niloticus) under high-fat diet (HFD) conditions. Using 16S rRNA gene sequencing of the V3-V4 region, we characterized shifts in microbial community structure and functional profiles across control, HFD, and OA-supplemented groups. The project aims to elucidate the role of the gut-liver axis in OA-mediated alleviation of hepatic steatosis and inflammatory responses in farmed fish.