Single cell transcriptomic analysis of skeletal muscle cells
Single cell RNA-sequencing was used to analyze the cellular heterogeneity of mononucleated, non-myofiber cell populations and their transcriptional states in post-injury skeletal muscle Overall design: Droplet-based scRNA-sequencing of non-myofiber mononucleated cells purified by fluorescence-activated cell sorting (FACS) from muscle samples of aged and young control female mice at 4 days post injury was performed.