Relative quantitative sequencing of 16SrRNA in the feces of sleep-deprived rats
The relative quantification results of 16S rRNA sequencing were used to determine whether stellate ganglion block could alter gut microbiota diversity, community composition, and metabolic products.The rats were randomly assigned to the CON group, sleep deprivation (SD) group , SD+SGB group. Rats in the groups receiving SGB treatment received stellate ganglion blockade on day 1, 3, 5, or 7 of the sleep deprivation procedure, corresponding to their group's designated deprivation period.