RNA-seq data of total RNA treated with different E. coli AlkB variants.
We compared several E. coli AlkB variants for their applications in facilitating sequencing of tRNA species in total RNA. These proteins are wild type AlkB, mutant D135S and mutant D135T. Overall design: Total RNA isolated from HEK 293T cells were treated with different combinations of enymes: (1) wild type alone, (2) wild type + D135S, (3) wild type + D135T, (4) no enzyme. The treated samples were then subjected to library construction and illumina sequencing.