Identification of a new HLA-C*16:02 a french in a volunteer bone marrow donor
Here we describe a novel C*16 :02 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:   HLA-C* :16New allele differs from C*16 :02 :01 :01 by one nucleotide substitution at position   of exon 253 4  (position 872 of cDNA), where  base C is replaced by T inducing Leu instead Pro (CCG>CTG) .