Klf2/4-independent effects of Pcdhg on gene expression in endothelial cells exposed to Oscillatory Shear Stress (OSS)
We found that Pcdhg is a potent suppressor of Klf2/4 in endothelial cells. We performed RNAseq analysis to characterize deregulated pathways and processes by which Pcdhg may regulate Klf2/4. Overall design: Control siRNA, Pcdhg siRNA or Pcdhg+Klf2/4 siRNA transfected HUVECs were stimulated with OSS for 16h, the RNA extracted and subjected to bulk sequencing.