RNA sequencing in the freshwater planarian Dugesia ryukyuensis
RNA sequencing was performed to examine gene expression patterns associated with sexualization essential genes in the freshwater planaria Dugesia ryukyuensis. In this project, sexualization and gene knockdown were performed simultaneously. Experiments were performed one at a time on the following three genes.(1)Drnhr-1 (nuclear receptor 2dbd gamma)KD individuals (2)Drdmd-1 (doublesex and mab 3 transcription)KD individuals (3)Drklf-1 (krÃ¼eppel-like factor 4 All worms had been fed chicken liver for at least one year prior to the experiment.