Comparison of the kidney transcriptome of 45 days old principal cell-specific Tsc1 knockout (Tsc1f/f/Aqp2 Cre), 45 days old principal cell-specific Tsc1 knockout/Foxi1 knockout (Tsc1/Foxi1 dKO), and 45 days old WT mice
Tuberous sclerosis complex is a genetic disorder that affects multiple organs. In the kidney, it presents as hamartomas and cysts. The epithelium of TSC renal cysts is primarily composed of A-intercalated cells. The transcription factor FOXI1 is indispensable in the development of intercalated cells. The expression of Foxi1 mRNA is significantly upregulated in the kidneys of various mouse models of TSC. The simultaneous ablation of Tsc1 and Foxi1 prevents the formation of cysts in the kidneys of Tsc1 KO mice. In these studies, we compared the renal transcriptomes of 45 days old principal cell-specific Tsc1 knockout, Tsc1/Foxi1 double knockout, and WT mice. Overall design: The kidney transcriptome of principal cell-specific C57BL/6 Tsc1 knockout mice (Tsc1f/f/Aqp2 Cre), principal cell-specific C57BL/ 6 Tsc1 and Foxi1 double knockout mice and WT C57BL/6 at 45 days of age were compared.