16S rRNA metagenomics molecular techniques to determine the microbial community in the fragmented electroactive microbial fuel cell (FAB-MFC)
Microbial fuel cells (MFCs) represent a promising technology for sustainable energy production and wastewater treatment, harnessing the metabolic activities of microorganisms to convert organic substrates into electrical energy. This study employs 16S rRNA shotgun metagenomics to analyze the microbial communities within MFCs, aiming to elucidate their composition, diversity, and functional dynamics. By sequencing the 16S rRNA gene, we identify both predominant and rare microbial species, providing insights into their phylogenetic relationships and potential roles in enhancing energy generation and substrate degradation.