Genetic suppression of precocious transcription termination identifies mutations in essential subunits of the fission yeast cleavage and polyadenylation machinery
RNA-seq anaysis compares gene expression of each mutant (cft1-L249Q, msi2-G252E, pfs2-E104A, pfs2-V180F, pla1-Y86L, pta1-T524M, and ysh1-L74F) to the wild-type fission yeast (Schizosaccharomyces pombe) Overall design: 7 test samples and 1 control sample were processed (each sample has 3 replicate experiments).