Gene expression during CCK-induced damaged pancreatic acinar cells with or without the addition of OCA
Obeticholic acid (OCA) has shown pharmacological protection activity in acinar cell injury and pancreatic necrosis in vitro. RNA sequencing was performed by extracting mRNA from CCK-induced damaged pancreatic acinar cells with or without the addition of OCA. Gene enrichment analysis by Kyoto Encyclopedia of Genes and Genomes (KEGG) identified oxidative phosphorylation in the top ten significantly changed pathways. Similarly, Gene Set Enrichment Analysis (GSEA) enrichment analysis revealed clear alterations in "mitochondrial ATP synthesis coupled electron transport", "mitochondrial respiratory chain complex assembly", "NADH dehydrogenase complex assembly", and "oxidative phosphorylation". Overall design: Fresh primary pancreatic acinar cells (PACs) were quickly isolated from mice using IV collagenase. PACs injury was induced by adding 1 µM cholecystokinin octapeptide (CCK) for 6 h. Cells were incubated with increasing concentrations of OCA(2.5µM).