Raw sequencing datas used in the "2. Original Case study: detection of uncharacterized taxa" of the Supplementary File 2 of the preprint: https://doi.org/10.64898/2025.12.04.692289
These raw sequencing datas are materials for the study "A FAIR Amplicon Sequencing Workflow for Long-term Environmental Monitoring". This paper describes a new bioinformatic tool dedicated to long-term environmental monitoring of microbial communities. The Reproducible Amplicon Sequencing Pipeline for Antarctic Monitoring (RASPAM) is built on Apptainer containers and Nextflow workflows. RASPAM implements Amplicon Sequence Variants (ASVs) and Zero-radius Operational Taxonomic Units (ZOTUs) to provide high-resolution taxonomic affiliation and sensitive description of microbial communities. It incorporates, in addition to the SILVA database, a taxonomically curated 16S rRNA database for cyanobacteria and enables comparisons against NCBI databases to facilitate the identification of rare prokaryotic strains in environmental samples. RASPAM is Findable, Accessible, Interoperable, and Reproducible (FAIR) and represents a robust tool for long-term monitoring of microbial communities in Antarctic and other extreme environments. The tool is also easily configurable, to adapt to various case studies.  The preprint associated to this dataset is accessible on BioRxriv: https://doi.org/10.64898/2025.12.04.692289