Mouse liver fxr chip-seq II
We analyzed genome-wide FXR binding in liver of mice treated with GW4064 by ChIP-seq Overall design: Freshly liver samples  was fixed in 1% formaldehyde for Cross-Linking of DNA and Protein. Liver was sonicated to fragment chromatin DNA.Chromatin was immunoprecipitated overnight at 4 °C using FXR antibody (Cell Signaling Technology, 72105) .The sequencing libraries of input and IP were generated by NEBNext®UltraTM DNA Library Prep Kit for Illumina.The library products corresponding to 1100-500 bps were enriched, quantified and finally sequenced on Novaseq6000 sequencer (Illumina) with PE150 model.