H4K20me1 and gene expression in adult myocyte hypertrophy
The lysine methyltransferase KMT5A is shown to mediate the effects of CDC14A in the adult cardiomyocyte by regulating H4K20 mono-methylation, such that reduced KMT5A expression promotes a phenotype resembling Dilated Cardiomyopathy. Overall design: Adult rat ventricular myocytes were transfected with control or KMT5A siRNA as indicated for 2 days and then treated with phenylephrine (20 µmol/L) or vehicle control for 1 hour before harvest.