RNA sequencing of colorectal tumor tissues from mice
Immunodeficient male BALBc nude mice were obtained from the Chongqing Pharscin InnoBio Experimental Animal Center. HCT116 CRC cells were subcutaneously injected into the right flank of each mouse. When palpable tumors had developed (day 7), the mice were randomly assigned to two groups (n = 4 per group) and administered intraperitoneal injections of Epimedium sagittatum derived extracellular vesicles (ESEVs) (0 or 22 mg/kg per mouse) once daily for 14 consecutive days. At the end of treatment (day 21), all mice were euthanized; tumors were excised and weighed. Tumor sample was used for transcriptomic analysis. To clarify molecular mechanisms underlying the antitumor effects of ESEVs, RNA sequencing was performed on colorectal tumor tissues from mice treated with ESEVs or NaCl (control). Eight cDNA libraries were generated, comprising four biological replicates per group.