Synaptic RNA modification of inosine and m1A in mouse brain
We compared inosine profile and m1A RNA modification profiles between homogenate and synaptosome fractions of mouse forebrain using ICE-seq and m1A-seq. Overall desing: Homogenate fraction (2 replicates) and synaptosome fraction (2 replicates) from mouse forebrain was isolated using sucrose gradient method followed by ICE treatment (mild and strong) for ICE-Seq along with non-treated samples serving as input. For m1A-seq we performed reverse transcription of isolated RNA from mouse forebrain (2 replicates) using modified enzyme RT1306 to incorporate mutations at m1A sites. RNA from two separate replicates reverse transcribed using Superscript III serve as input.