Bulk RNA sequencing of endometrial organoids in the IVMC protocol
Six independent endometrial organoid cultures were subjected to the in vitro menstrual cycle protocol (IVMC), which recapitulates the events of differentiation, hormonal withdrawal, breakdown, repair and regeneration. Samples were collected at various timepoints (Pre-diff, P4-diff, Horm Withdr 24h, Horm Withdr 48h, Repair 24h, E2-diff 24h, E2-diff 48h) and analysed with bulk RNAseq to validate the protocol and investigate the dynamic transcriptomic changes across timepoints.