Effect of targeting NOS2 on gene expression of triple-negative breast cancer (metaplastic) SUM159 cell lines
To investigate the role of iNOS in tumor cell morphology and gene expression, we utilized CRISPR-Cas9 gene editing (NOS2 Double Nickase Plasmid, Santa Cruz) to knockout NOS2 in SUM159 cells. After puromycin selection, resistant clones were selected by immunoblotting for iNOS expression and nitrite/nitrate colorimetric assay. We then performed gene expression profiling analysis using data obtained from RNA-seq of parental SUM159 cells compared to NOS2KO SUM159 cells. Overall design: RNA-seq of parental SUM159 cells compared to three clones of NOS2KO SUM159 cells.