A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood stage samples
Here we have designed a full processing pipeline of P. falciparum RNA that innclues RNA preservation, extraction, cDNA synthesis and amplification, globin depletion and final transcriptome readout. To validate the protocol we have analyzed transcriptomes of  P. falciaprum samples derived from filed studies as well as 3D7 lab strains. Overall design: Samples were extracted using high-throughput techniques adied by automatics liquid handlers, cDNA was amplified and resulted transcriptomes were analyzed and validated using RNA-seq platform. Some samples were globin depeted in vitro using novel CRISPR-Cas9 depletion method.