Next Generation Sequencing Facilitates Quantitative Analysis of Negative control/si-PDLIM3/si-PDLIM5/si-LMO7 Transcriptomes
The PDZ-LIM superfamily member is present in the nucleus of many cells and  consists of one PDZ domain at the N-terminus and one or more LIM domains at the  C-terminus. The family includes 10 genes, of which PDLIM3, PDLIM5 and LMO7 genes  have been shown to promote mouse myoblast differentiation and participate in the  regulation of skeletal muscle growth and development. To explore whether chicken  PDLIM3, PDLIM5 and LMO7 genes have the same function, this study explored the  expression of these genes in the before and after birth of chicken,  and after the chicken skeletal muscle satellite cells cultured in vitro interfered with the  expression of these genes. The proliferation and differentiation of  satellite cells were finally analyzed by transcriptome sequencing. The molecular  pathways involved in the regulation of cell proliferation and differentiation were  analyzed. The main results are as follows:  Transcriptome sequencing revealed that the differentially expressed genes of  chicken skeletal muscle satellite cells interfering with PDLIM3 gene expression were  mainly enriched in cGMP-PKG, Oxytocin, MAPK, Tight junction and other signaling  pathways, and involved in cytoskeletal protein binding, actin binding, muscle tissue  regeneration and development, adhesion and other functions. Transcriptome sequencing revealed that compared with the control group, the  differentially expressed genes of chicken skeletal muscle satellite cells that interfered  with PDLIM5 gene expression were mainly enriched in signaling pathways such as  Tight junction, Oxytocin, p53, MAPK, and involved in myocyte production and  muscle contraction. Muscle cell differentiation, and participate in cytoskeletal protein  binding, actin cytoskeleton formation, actin and actin binding. Overall design: mRNA profiles of si-PDLIM3, si-PDLIM5, si-LMO7 and negative control satellite cells