Thrombospondin-1 promotes fibro-adipogenic stromal expansion and contractile dysfunction of the diaphragm in obesity II
This study defines the impact of in vivo thrombospondin-1 (THBS1) ablation on diaphram stromal cell profile, histology and contractile funciton in a mosue model of diet-induced obesity Overall design: Bulk RNA sequencing performed on tissue samples of daiphragms from wild type C57 mice and thrombospondin-1 knockout mice fed high fat diet (45% kcal from lipid) for 6 months