Comparative analysis of adipose-, bone marrow-, and tendon-derived MSCs for Achilles tendon Regeneration
RNA sequencing (RNA-seq) was performed on adipose-derived, bone marrow-derived, and tendon-derived mesenchymal stem cells (MSCs) (n = 3 per group) to compare their baseline transcriptomic profiles. RNA was extracted (RIN > 7), libraries were prepared using the TruSeq Stranded Total RNA kit, and sequencing was performed on an Illumina platform (paired-end, 2x100 bp). Reads were quality filtered (Phred Q30), aligned to the Rattus norvegicus genome (rn6) using HISAT2, and analyzed with DESeq2 for differentially expressed genes (log2 fold change greater than or equal to 1, p < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified key biological functions and signaling pathways.The FASTQ files contain raw paired-end sequencing reads with an average read length of 100 bp, representing transcriptomic variations among MSC types.