Deciphering the impact of RAC1-SPTAN1 in ARPKD cystogenesis using multifaceted models
RNAseq of kidney organoids derived from stem cells with SPTAN1 mutation and cultured under flow conditions. Overall design: Total RNA was isolated from flow-cultured kidney organoids using TRIzol from two biological replicates per condition (SPTAN1+/+ clone 1, SPTAN1+/+ clone 2, SPTAN1+/- clone 1, SPTAN1+/- clone 2). RNA-Seq data was generated using the NovaSeq X Plus (Novogene).