Isolation of Lacticaseibacillus rhamnosus from Gir cow milk sample
Herein, we isolated and characterized probiotic potentials of Lacticaseibacillus rhamnosusBIONCL3 strain from Gir-cow milk (Indian-born dairy cattle breed), Baramati,Maharashtra, India. This strain, exhibited excellent antimicrobial activity, acid, bile, metaltolerance and non-haemolytic in nature. The BIONCL3 strain is capable of producingindole-3-propionic acid, succinic acid, and benzoic acid. The structure elucidation of these metabolites were characterized through extensive 1D NMR and 2D NMR (HSQC, HMBC, and NOESY) analyses, along with high-resolution mass spectrometry (HR-MS). Notably, bis(2-ethylhexyl) phthalateis reported for the first time in L. rhamnosus. The BIONCL3 strainresilience and safety profile support its strong probiotic in food and pharmaceuticalapplications. Also we have characterized this strain through whole genome sequencing.