Heligmosomoides polygyrus RNA sequencing
Total RNA from adult Heligmosomoides polygyrus was isolated using the miRNAeasy kit (Qiagen) and visualized on a bioanalyzer (RIN > 7.0) prior to library preparation using TruSeq Stranded Total RNA Library PrepGold with ribodepletion. Libraries were sequenced using NextSeq 2000 platform for 100 cycles by Edinburgh Genomics. The resulting short-read RNA-seq data were used to predicted protein-coding genes in the ngHelPoly1.1 reference genome.