Visium spatial transcriptomics sequencing of the skin of cGVHD mice
Cutaneous chronic graft-versus-host disease (cGVHD) is a severe complication of allogeneic stem cell transplantation(allo-HSCT), characterized by fibrosis and immune dysregulation. The dysregulated activation and proliferation of donor T, B cells, and macrophages, leading to the development of autoantibodies and fibrosis, is central to the pathogenesis of cGVHD. Phosphoinositide-3 kinase (PI3K), a key lipid kinase, plays a critical role in the activation and function of multiple immune cells, making it a potential target for therapeutic intervention in cGVHD. As a result, this study aimed to evaluate the efficacy of PI3K inhibitors in the treatment of cGVHD. Three skin samples of cGVHD mice and four skin samples of cGVHD mice treated with PI3K inhibitor were paraffin-embedded, sectioned and transferred onto the Visium Spatial Gene Expression Slides (10x Genomics).