Enterococcus faecalis OG1RF Experimental Evolution at pH 4.8, pH 7.0 and pH 9.0 January 10, 2023
Enterococcus bacteria inhabit human and soil environments that show a wide range of pH. Strains include commensals as well as antibiotic-resistant opportunistic pathogens. We investigated adaptation to pH stress in the model strain E. faecalis OG1RF by conducting experimental evolution in brain-heart infusion medium buffered in acid (pH 4.8), neutral pH (pH 7.0), and base (pH 9.0). We also investigated biofilm evolution at pH 9.0.