RNA-seq of Recombinant Mouse Cold-inducible RNA-binding protein(rmCIRP)-treated Naïve CD4+ T cells
Cold-inducible RNA-binding protein (CIRP) is a ubiquitous intracellular RNA chaperon, which serves as a damage-associated molecular pattern (DAMP) when released outside the cells upon stimulation by inflammation and infection. It has been shown that CIRP promotes splenic CD4+ and CD8+ T-cell activation in vivo and predisposes CD4+ T cells to a Th1 hyperinflammatory response profile in vitro. However, the effect and underlying mechanisms of CIRP on Th17/Treg differentiation is still unclear. This study reveals the transcriptomic changes of mouse Naïve CD4+ T cells induced by Recombinant Mouse CIRP (rmCIRP) in vitro. Overall design: Mouse Naïve CD4+ T cells activated by plate-bound anti-CD3 (2 µg/ml) and anti-CD28(1 µg/ml). Then, Naïve CD4+ T cells were cultured in the presence of rmCIRP(1µg/ml)or equal volume PBS. Each group contained 3 replicates. After 24h, cells were collected, and subjected to RNA-seq.