RNAseq of transfected protoplasts of  Populus trichocarpa
PtrHSFB3-1 and PtrMYB092 are xylem specific genes in xylem of P. trichocarpa, and their expression levels are down regulated most significantly in tension wood. These two transcription factors were transiently overexpressed in stem differentiating xylem (SDX) of P. trichocarpa , and transcriptomic sequencing was performed to identify the regulatory effects of the two transcription factors on wood formation related genes. Overall design: We incubated SDX protoplast of P. trichocarpa transfected with GFP, PtrHSFB3-1 and PtrMYB092  genes for 12h to insight into the regulation of these two genes on wood formation related genes