Transcriptomic Profiling of Glioma Stem Cell-Neuron Co-Culture Systems: Elucidating the Role of C1QL1 in Malignant Synapse Formation and Tumor Microtube Outgrowth
Primary mouse cortical neurons were cultured for 7 days in poly-D-lysine-coated plates and co-cultured with dissociated GSCs for 3 days to model neuron-glioma interactions. Neuronal medium was partially refreshed every 48 hours.