Off-target effect of CWG-cPIP on gene expression of human fibroblasts
CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in cells, CWG-cPIP (1 µM) was treated to human fibroblasts. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-treated human fibroblasts 7 days later, mixing with ERCC RNA spike-in controls. Overall design: Comparative gene expression profiling analysis of RNA sequencing data for the human fibroblasts treated with vehicle or CWG-cPIP (n = 3 replicates each).