Genome-wide identification of transcriptional targets for PSMD14 and NSD2 in LP-1 cells
To investigate the genome-wide transcription targets of PSMD14-NSD2 complex and to explore the gain-of-function of PSMD14-NSD2 complex in multiple myeloma, cleavage under targets and tagmentation (CUT&Tag) was performed in LP-1 cells using antibodies against PSMD14 or NSD2. Following CUT&Tag,  DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE. Overall design: Examination of transcriptional targets for PSMD14 and NSD2 in LP-1 cells