Type I Interferon (IFN-alpha)-Induced miRNA Expression Profile in Raji Cells (PRJCA036045)
Systemic lupus erythematosus (SLE) is characterized by type I interferon signature closely associated with abnormal B cell activation. While miRNAs are known to participate in type I interferon signaling networks, their involvement in B cell immune responses remains unclear. This project focuses on the dynamic miRNA regulatory network in B lymphoma cells (Raji) under IFN alpha stimulation. Raji cells were cultured in vitro and induced with 1000 IU/mL IFN alpha for 12 hours, with three biological replicates and EBV negative controls (Ramos cell line). RNA was extracted and subjected to small RNA sequencing using the Illumina SE-50 platform. Raw reads underwent quality control followed by identification of differentially expressed miRNAs using miRDeep2. The study aims to elucidate the molecular mechanisms of miRNA-mediated immune responses in SLE, potentially revealing novel diagnostic and therapeutic targets by comprehensively analyzing the miRNA expression profile under type I interferon stimulation.