Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1
We conducted snRNAseq to investigate the transcriptomics of postmortem prenatal and peirnatal human brains. Overall design: Single nuclei suspension were prepared from whole (GW22-23), periventricle (GW30-PW3), or cortex(GW30-PW3) regions of postmortem huamn tissues. DAPI+, PU.1+ and OLIG2+ nuclei have been sorted with Fluorescence-activated cell sorting (FACS) and sequenced.