Draft genome sequencing of the European medicinal leech Hirudo medicinalis ROMIZI 11733
A single specimen of the European medicinal leech Hirudo medicinalis (specimen coucher ROMIZI 11733) collected in August 2016 from an unnamed pond close to the town of Gricek pri Željnah in sourheastern Slovenia. DNA was extracted from full body tissue avoiding the integument, as this tissue has been shown to exacerbate library creation. That is, the DNA isolate was derived from gonadal, nervous and muscular tissue in a single extract. The tissues were frozen with liquid nitrogen and macerated using a pestle in a RNase-free Eppendorf tube. DNA was extracted using proteinase K digestion overnight, followed by a standard phenol-chloroform extraction protocol. The extracted DNA was sequenced over half a lane on a HiSeqX platform with 2×150 paired-end reads using the 10X Genomics Chromium methodology at the New York Genome Center (New York, NY, USA).