ChIP-seq on INTS11-degron cells
Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations. To further determine the effects of COMPASS subunits degradation on H3K4 methylations occupancies genome-wide, we performed time-course spike-in chromatin immunoprecipitation and sequencing (ChIP-seq, also known as ChIP-Rx) analysis in both degron systems. Overall design: INTS11-FKBP dTAG degron tag into the 3' end of both alleles of endogenous Ints11 locus of the E14 mESC cell line.