Engineering IgG1 Fc specificity and affinity for Fc gamma receptors using deep mutational scanning titrations
We made barcoded libraries of IgG1 Fc, each containing ~4,350 distinct single amino acid mutations. We then applied deep mutational scanning technology to quantify IgG1 Fc mutational impact on specificity and affinity for various Fc gamma receptors. We used a lentiviral system for expression of IgG1(216-447) on the surface of mammalian HEK293T cells.