Adipose Fin Resection alters Cerebral Transcriptome of Rainbow Trout
Brain transcriptomes were recorded in 20 juvenile Rainbow trout telencephalon and midbrain regions at 3 and 72 hours after adipose fin resection (FCF group) in comparison to non-resected (IF) controls and unchallenged (UCTR) controls. Fish were exposed to a forced swimming regime with parallel video recording of respiratory frequencies. Besides brain monoamine levels (noradrenaline, dopamine, serotonin, and metabolite HVA, DOPAC) and blood levels of glucose, lactate, cholesterol, triglycerides, cortisol, adrenocorticotropic hormone were measured. After killing by an overdose of 2-phenoxyethanol (0.7 ml/l), followed by dissection of the vertebral column at skull level, the fish brain was quickly and carefully dissected into three parts transversally and perpendicular to the rostral-caudal axis: forebrain (FB) section without olfactory bulb, midbrain (MB) section without pituitary and hindbrain (HB) section composed of cerebellum and brainstem. Each part was than longitudinally separated into two equal halves, separately weighed and snap-frozen in liquid nitrogen for further analysis. One halve of each part was used for monoamine analysis, the other for RNA isolation.