Bulk RNA sequencing for K5-Cre Sox2 mice and K5-Cre Sox2 IL1R KO mice following cancer development in the buccal mucosa
We painted the oral mucosa of mice with tamoxifen suspended in corn oil for 5 days. We monitored the mice by checking their body weight every MWF. We euthanized the mice after observing a drop of 20% initial body weight. We collected the buccal mucosa for RNA extraction.