Genome-wide identification of transcriptional targets for H2AK119ub and H3K36me2 in LP-1 control and PSMD14 knockdown cells
To investigate the PSMD14-NSD2 complex track with H2AK119ub or H3K36me2, CUT&Tag was performed in LP-1 cells infected with lentiviruses carrying control shRNA or PSMD14 shRNA using antibodies against H2AK119ub or H3K36me2. Following CUT&Tag,  DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE. Overall design: Examination of transcriptional targets for H2AK119ub and H3K36me2 in LP-1 control and PSMD14 knockdown cells