Genome-wide expression analysis in Control and Atg5-deficient eosinophils.
Depletion of autophagy-associated protein 5 (Atg5), specifically within the eosinophil lineage in mice, resulted in increased body weight, impaired glucose metabolism, and structural alterations in adipose tissue. Our findings highlight that Atg5 influences the functional activity of eosinophils within adipose tissue rather than their quantity. To gain a deeper understanding of the molecular mechanisms underlying ATG5's role in eosinophils, we conducted total RNA-seq analyses on control and Atg5-deficient eosinophils isolated from both bone marrow and blood of mice. Overall design: RNA expression profiling of control and Atg5-deficient eosinophils isolated from bone marrow and blood of threeAtg5flox/floxeoCreIl5tg mice and three eoCreIl5tg mice as control.