Transient expression of SlySBP12a in Nicotiana benthamiana leaves
To investigate the transcriptional programs associated with SlySBP12a-induced PCD, we performed RNA-sequencing on Nicotiana benthamiana leaves transiently expressing SlySBP12a or EV control. Samples were harvested at pre-symptomatic stages, prior to tissue collapse, to capture the regulatory phase of the death process. This experimental design enabled us to identify core genes and pathways activated during SlySBP12a-induced PCD and explore how the transcriptional programs driven by SlySBP12a integrate with broader networks of stress, hormone, and defense signaling.