Identification of a new DRB1*15:01 in a french  volunteer bone marrow donor
Here we describe a novel DRB1*15 :01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*29:02 ,*32:01 B*18:01 ,*44:03 C*12:03 ,*16:01 DRB1*13:01 DRB1*15:01New DRB3*02:02 DRB5*01:01 DQA1*01:02 ,*01:03 DQB1*06:03,*06 :446 DPA1*02:01 ,*02:01 DPB1*14:01 ,*-  HLA-DRB1*15 :01New allele differs from DRB1*15 :01 :01 :03 by one nucleotide substitution at position 103   of exon 4  (position 755 of cDNA), where  base A is replaced by G inducing Ser instead Asn (AAT>AGT) .