Tetraselmis suecica microbiome metatranscriptomics and metagenomics
A xenic Tetraselmis suecica culture was supplied with either Phaeobacter inhibens DSM17395 or Phaeobacter piscinae S26 to study the impact of the secondary metabolite tropodithietic acid (TDA) on the native microbiome of the microalga. After co-cultivation for 1, 2, or 4 days, RNA and DNA was co-extracted for metatranscriptomics and metagenomics. RNA was treated with TURBA DNA-free Kit (Invitrogen) and rRNA was depleted before reverse transcription and PCR amplification. RNA and DNA was sequenced by Novogene using an Illumina Novaseq 6000 instrument.