Transcriptome profiling of lungs of hamsters infected with various SARS-CoV-2 variants
To characterize the difference of the effects caused by viral infections among SARS-CoV-2 variants, we profiled the transcriptome of the lungs of SARS-CoV-2-infected hamsters. Golden hamsters were infected with clinical isolates of various SARS-CoV-2 variants (B.1.1 and Omicron BA.1, BA.2, and BA.5). At days 2 and 5 post-infection, the infected hamsters (n = 4 for each timepoint) were scarified, and total RNA was extracted. Library for mRNA sequencing was prepared using Illumina TruSeq Stranded mRNA Sample Preparation Kit. Pair-ended, 150-base pair sequencing was performed using NovaSeq 6000 System