Functional and mechanistic study of the inhibitory role of the antisense long non-coding RNA (IRF1-AS1) in regulating the transcriptional activity of IRF1 and M1 polarization of macrophages in ARDS.
Through high-throughput sequencing and further cell and animal experiments, we found that the antisense long non-coding RNA of IRF1 (IRF1-AS1) binds to the intronic region of IRF1, inhibiting its transcriptional activity and thereby regulating macrophage activation and inflammation-induced lung injury in ARDS. Overall design: CD14 cells were obtained from our hospital's biobank. After 48 hours of in vitro colony-stimulating factor induction, total RNA and DNA were extracted from cells treated with LPS and IFN? for 24 hours. ChIP-seq/ATAC-seq and RNA-seq were performed using specific antibodies and assay kits.