Precision digital mapping of genomic DSBs by INDUCE-seq
We have developed INDUCE-seq, which simultaneously detects the presence of low-level endogenous DSBs caused by physiological processes, and higher-level recurrent breaks induced by restriction enzymes or CRISPR-Cas9 nuclesase. INDUCE-seq exploits an innovative NGS flow-cell enrichment method, permitting the digital detection of breaks in the genome.