eCLIP-seq analysis of PF3D7_0823200 in Plasmodium falciparum
We used an unbiased proteome-wide approach, called R-DeeP, a method based on sucrose density gradient ultracentrifugation, to identify RNA-dependent proteins. One candidate, PF3D7_0823200, was functionally characterized and validated as a true RBP. Using enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP-seq), this protein was validated as a regulator of various Plasmodium non-coding transcripts including var genes and ap2 transcription factors.