Identification of a new A*03:01 allele
Here we describe a novel A*03 :01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*03:01New ,*32:01 B*38:01 ,*52:01 C*12:02 ,*12:03 DRB1*13:01 ,*15:02 DRB3*01:01 DRB5*01:02 DQA1*01:03 ,*01:03 DQB1*06:01 ,*06:03 DPA1*01:03 ,*02:01 DPB1*04:01 ,*26:01 HLA-A*03 :01New allele differs from A*03 :01 :01 :01 by one nucleotide substitution at position  109  of exon  4  (position 728 of cDNA), where  base G is replaced by A inducing Gln instead Arg (CGG>CAG) .