Human adipose tissue single-nucleus RNA-sequencing before and after lifestyle intervention
Single-nuclei RNA sequencing (snRNA-seq) data of human adipose tissue. The samples are a part of a study investigating the effect of Lifestyle intervention on human adipose tissue. The 10 participants represented in this study were severely obese at project start with a mean BMI of 46.0 ± 3.1 kg/m2 and a BMI range of 31.4–63.0 kg/m2, with a 50/50 female/male ratio. After intervention mean BMI was 40.8 ± 2.8 kg/m2 with BMI range: 27.4–57.5 kg/m2. These participants took part in 15-weeks of consecutive lifestyle intervention consisting of a hypocaloric diet and moderate physical activity. Hypocaloric diet was calculated for the specific participant and calculated to reduce the subject's body weight by approximately 1% per week. The exercise consisted of 2-3 hours of moderate-intensity physical activity (e.g. walking or swimming) 5 days per week. The specific details are reported in Bruun et al. Am J Physiol Endocrinol Metab, 2006 (https://doi.org/10.1152/ajpendo.00506.200). The snRNA-seq data contains nuclei isolated from 19 available biopsies with 9 samples before intervention and 10 samples after intervention.The RNA quality of one of the baseline samples was too low to proceed with snRNAseq (sample J "before" was excluded in the manuscript, but is included here).