Whole transcriptomes analyses of control and Rictor knockout intestinal epithelial cells
To explore the molecular mechanisms of mTORC2, RNA sequencing was performed to analyze the geome-wide change of Rictor knockout intestinal epithelial cells compared to those of control intestinal epithelial cells. Overall design: Methods: Total mRNA was extracted from control and Rictor knockout intestinal epithelial cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays.