Chromatin organization and behavior in euchromatin and heterochromatin: Sequencing of H3.2-HaloTag incorporated genomic regions.
Recent advanced imaging studies have revealed that euchromatin in higher eukaryotic cells mainly forms condensed liquid-like domains, rather than open ones. While such chromatin organization provides a higher-order regulation of genome functions, it raises intriguing questions: How are euchromatin and heterochromatin physically different in living cells? To approach this question, we have developed a novel chromatin labeling method, replication-dependent histone labeling (Repli-Histo labeling), which can specifically label four groups of genome regions from euchromatin (early replicated regions) to heterochromatin (late replicated regions) based on DNA replication-timing. In this project, we purified the labeled chromatin regions in euchromatin and heterochromatin, sequenced them, and determined where their genomic regions were.