miRNA profiling in human nonsteatotic and NAFLD livers
To investigate the function of miRNAs in liver, we obtained liver tissues from nonsteatotic individuals and fatty livers from patients with nonalcoholic fatty liver disease (NAFLD). Patients due to excessive alcohol consumption, autoimmune liver disease, viral hepatitis and diabetes were excluded. Nonsteatotic livers were collected from the normal region of the livers from donors who received liver resection due to liver hemangioma, and were defined as those with NASH activity scores of 0  We then performed miRNA sequencing using livers from two NAFLD patients and two nonsteatotic individuals. Overall design: Comparative miRNA level profiling analysis of liver tissues from human non-steatotic and NAFLD livers.