MNase-Seq analysis of wild-type Candida glabrata strain in RPMI-growth and macrophage-internalized conditions at 2 h and 10 h post-infection.
MNase-sequencing analysis led to identification of 56,637 to 71,823 nucleosomes under RPMI-growth or macrophage-internalized conditions. Mapping of dynamic nucleosomes revealed that 12 to 24% of total nucleosomes were altered in their position, occupancy or fuzziness. Notably, while the nucleosome position shift was the most common dynamic event, the nucleosome fuzziness was the least observed change. Promoter regions were found to be nucleosome-depleted. Overall design: The study was designed to delineate the effect of the macrophage internal milieu on the chromatin dynamics in C. glabrata. C. glabrata wild-type cells were grown either in RPMI medium or infected to human THP-1 macrophages. After 2 h and 10 h, C. glabrata cells were collected, and chromatin structure was analysed via MNase-Sequencing approach. The experiment was performed with two biological replicates.