Single cell RNA sequencing data from a complete human embryo at Carnegie stage 16 (sample ID: F147 for Haniffa Lab; 15942 for HDBR)
"Investigating the blood, immune and stromal cells present in a human fetal embryo in a world first single cell transcriptomic atlas. The embryo was dissected into 12 coronal (3x4) sections of ~2mm^3 each, live single cells sorted, with cell suspension then undergoing 10x chromium 5 prime scRNA-seq. The broad section descriptors are as follows: section one (lower vertebrae), two (lower vertebrae and yolk sac stalk), three (mid-low vertebrae), four (liver), five (mid vertebrae), six (internal organs), seven (brain/hear/eyes), eight (upper vertebrae), nine (internal organs), ten (brain/heart/eyes), eleven (brain/stroma), twelve (brain/stroma). Lane \""WS_wEMB11031943\"" was excluded from downstream analysis due to low cell numbers post-CellRanger QC. Termination procedure for this embryo was medical. The F147_[features...barcodes...matrix].[tsv...mtx].gz files attached to this accession represent raw count data from all the 10x lanes in this accession combined, and as output from CellRanger filtered matrices (CellRanger version 6.0.1 using human reference genome GRCh38-2020-A)."