Transcriptomic Analysis of a Pichia pastoris (syn. Komagataella phaffii) Engineered Strain
Human granulocyte-macrophage colony-stimulating factor (hCSF2) is a clinically important therapeutic protein with growing market demand, necessitating the development of efficient recombinant expression platforms. Pichia pastoris (syn. Komagataella phaffii) represents a well-established yeast chassis for recombinant protein production and offers considerable potential for systematic engineering. In this study, to further improve protein production, comparative transcriptomic analysis was employed to identify and subsequently enhance cellular pathways involved in protein folding and transport.