Effects of HLA DRB1*15:01 interactions with ?-syn32-46 on gene expression in the ileum
HLA DRB1*15:01 is overrepresentated in Parkinson's disease patients and binds with high affinity to the ?-synuclein peptide, 32-46. Immunization of humanized mice expressing HLA DRB1*15:01 with ?-syn32-46 induces enteric phenotypes similar to those of prodromal Parkinson's disease. We collected the ileum from HLA mice immunized with either Complete Freund's Adjuvant (CFA) with ?-syn32-46 or CFA alone, and sequenced the tissue with bulk RNA sequencing at 21 days post immunization to determine changes in gene expression. Overall design: 10-12 week old HLA DRB1*15:01 expressing MHC-II (-/-) mice were immunized with ?-syn32-46 (100ug) in CFA (200ug) or CFA alone. At 14 days post immunization (DPI), mice were boosted again with the same emulsion followed by an injection of pertussis toxin (400ng), then an additional pertussis toxin injection at 16 DPI. At 21 DPI, the ileum was collected into TRIzol reagent for bulk RNA sequencing