scRNA-seq of control and Etv1 mutant distal small intestinal organoid cultures
To characterize the effect of mutating Etv1 on enteroendocrine cell specification and hormone expression, we performed scRNA-seq experiments analysing cells from control and Etv1 mutant organoid cultures from mouse distal small intestine. We identified 6 transcriptionally distinct clusters, that had a similar cellular contribution from controls and Etv1 mutants. In the cluster identified as enteroendocrine cells, we observed differences in hormone expression between controls and Etv1 mutants. Overall design: Five samples containing 2 biological replicates from control cultures and 3 biological replicates from mutant Etv1 cultures, subjected to cell sorting and tagged using hashtag oligos. Subsequently, samples were pooled and sequenced with Illumina NovaSeq 6000 platform.