High and low temperature acclimation enhances extreme temperature adaptability of Cryptolestes ferrugineus and its molecular mechanism
Cryptolestes ferrugineus is one of the most difficult worldwide grain storage pests to eliminate completely at present. Its large accumulation leads to heating and dampness in grain storage, induces the accumulation of other grain storage pests, causes grain mildew and other problems, which seriously affects the safe storage of grain. Due to the long-term unreasonable use phosphine fumigants, causing C.ferrugineus such as the high resistance of phosphine, so urgently need green grain storage pest prevention and control technology as an alternative. Temperature control is a green prevention and control technology popularized at present. However, studies have found that insects can adapt to temperature, which leads to a longer time for them to be completely killed at the lethal temperature, seriously affecting the effectiveness of insect killing. This paper takes C.ferrugineus as the research object, aiming at the phenomenon of its adaptability to extreme temperature after cold and heat acclimation as the research direction, by observing the changes of semi-lethal temperature (LT50) before and after acclimation, using high-throughput transcriptome sequencing technology and RT-qPCR technology to screen out related genes, and using RNAi technology to verify the gene function. The reasons for the adaptability of C.ferrugineus after short-term temperature acclimation were expounded from the molecular level, which provided data and theoretical support for a more scientific and effective temperature insecticidal treatment scheme.