PacBio HiFi sequencing of single Drosophila melanogaster individuals
PacBio HiFi sequencing library was generated by the Tn5 tagmentation based Low-input and Low-cost amplification-free library-production method for PacBio Long-read sequencing (3L-seq). Each HiFi sequencing library with ~100ng DNA input from one single male Drosophila melanogaster. 19bp adapters in both 5' and 3' end of all reads were removed.