BRD4 Prevents the Accumulation of R-loops and Protects Against Transcription-Replication Collision Events and DNA Damage
Brd4 is essential in enhancing productive polII elongation along gene bodies. Inhibition of Brd4 leads to transcription-replciation conflicts and DNA damage. Overall design: ChIP-seq were performed for anti-Brd4, anti-gH2AX, anti-RNApolIIser2,  and anti-IgG from HeLa cells treated with DMSO or JQ-1 for 16 hours.