Bulk RNA-seq of HBMEC, iBMEC-like cells, and ETS-iBMEC in Response to Plasmodium falciparum Egress Products
This dataset comprises bulk RNA sequencing (RNA-seq) data generated from primary human brain microvascular endothelial cells (HBMECs), induced pluripotent stem cell (iPSC)-derived iBMEC-like cells, and ETS-transcription factor-guided iPSC-derived endothelial cells (ETS-iBMEC). Cells were cultured as monolayers and exposed to egress products from ruptured Plasmodium falciparum schizonts (5x107 schizonts/ml) or a media control. The experimental design included exposure durations of 8 and 24 hours to examine transcriptional responses over time. RNA was extracted from these samples and subjected to library preparation and bulk RNA sequencing using a NextSeq 2000 platform (P3 flow cell, 50 bp single-end reads, no barcoding). This dataset provides valuable insights into the transcriptional responses of endothelial cell types to P. falciparum egress products and serves as a resource for studying the host-pathogen interactions at the brain-endothelial interface and for endothelial profiling of the three cell types.