Metagenomic analysis of an upflow hydrolysis-acidification reactor treating BTEX-containing cabin-washing wastewater
This project provides metagenomic sequencing data from an upflow hydrolysis-acidification reactor (UHAR) treating simulated cabin-washing wastewater containing refractory benzene, toluene, ethylbenzene, and xylenes (BTEX). The study aimed to evaluate a coupling strategy integrating iron-carbon (Fe-C) composite addition with Computational Fluid Dynamics (CFD) guided flow field optimization. Genomic DNA was extracted from sludge samples across different operational stages -- including seed sludge, blank control, Fe-C addition, and Fe-C with optimized flow -- and sequenced on the Illumina NovaSeq platform (150 bp paired-end). The metagenomic data aims to reveal the underlying microbial mechanisms, focusing on how the synergistic system reshapes the metabolic network, enriches electroactive Geobacter and aromatic-degrading Acinetobacter, and stimulates Direct Interspecies Electron Transfer (DIET).