Single-nucleus RNA-seq of human macula in dry age-related macular degeneration
This Project utilised human maculae from dry AMD and age-matched non-AMD subjects and performed single-nucleus RNA sequencing (snRNA-seq). Libraries generated were sequenced on an Illumina NovaSeq X Plus platform using a 25B flow cell (10X Genomics). The study aims to investigate whether retinal ganglion cells (RGCs) undergo degeneration in dry AMD and, if so, how it differentially affects the predominant RGC subtypes. The database not only enabled the identification of cell-type-specific gene expression across multiple cell types within the macular region, but also facilitated the identification of preservation of total RGCs and predominant subtypes within the dry AMD maculae. Thus, understanding this cellular loss defines a critical window for intervention before irreversible vision loss with progression to neovascular AMD.