Sequencing of U18-treated yeast of different genetic background
This analysis correspond to the transcripts of four different yeast strains (NA, WA, WE, and SA) untreated and treated with the Ncr1p inhibitor U18666A Overall design: RNA-seq profiling of four yeast strains (DBVPG6044 [West African, “WA”: Mat alpha ho::NatMX, ura3::KanMXY12], Y12 [Sake, “SA”: Mat alpha ho::NatMX, ura3::KanMX], YPS128 [North American, “NA”: Mat alpha ho::NatMX, ura3::KanMX], and DBVPG6765 [Wine/European, “WE”: Mat alpha ho::NatMX, ura3::KanMX]) was conducted under control and U18666A-treated conditions. Yeast cultures were grown in triplicates at 28°C to mid-log phase (OD600 ~0.8) in YNB medium (control) or YNB medium with U18666A (200 µg/mL). Cells were centrifuged and treated with zymolyase (2U) for 30 minutes at 37°C. Total RNA was extracted using the E.Z.N.A. Total RNA Kit I (OMEGA), followed by DNase I treatment (Promega) to remove genomic DNA. RNA integrity was verified using a Fragment Analyzer (Agilent). Library preparation and sequencing were performed by BGI using DNBseq™ technology.