Transcriptional profiling of microglia versus brain engrafted monocyte-derived macrophages
To dissect the transcriptional differences between brain-engrafted MDMs and YS-derived microglia, we isolated tdTomato+ MDMs and tdTomato- YS-derived microglia from Ms4a3Cre:R26-tdTomato mice after PLX-3x treatment, followed by one month of repopulation.