hvscar-b,c transcriptomics
This study investigates the transcriptional impact of HvSCAR-B and HvSCAR-C mutations in barley (Hordeum vulgare) using RNA sequencing (RNA-Seq). HvSCAR proteins are components of the SCAR/WAVE complex, which regulates actin cytoskeleton dynamics and plays important roles in plant development and susceptibility.  We performed comparative transcriptome analysis between hvscar-b,c mutant plants and corresponding azygous wild-type controls (HvSCAR-B,C). Plants were grown for 8 days on 1% Phytoagar under controlled conditions. Whole root systems were harvested from four biological replicates per genotype, and total RNA was extracted for sequencing.  The resulting dataset provides genome-wide gene expression profiles to identify transcriptional changes associated with loss of HvSCAR-B and HvSCAR-C function in barley roots.