16S rRNA gene sequencing analysis of gut microbiota changes induced  by Rothia mucilaginosa in a mouse model of constipation
Background: Constipation is a common gastrointestinal disorder, and  emerging evidence suggests that gut microbiota plays a crucial role  in its pathogenesis. Mendelian randomization analysis has identified  Rothia mucilaginosa as a potentially protective factor against  constipation, but experimental validation is lacking.  Objective: This study aimed to investigate the effects of Rothia  mucilaginosa supplementation on gut microbiota composition in a  loperamide-induced constipation mouse model.  Methods: Twenty-six male C57BL/6 mice were randomly divided into three  groups: normal control (NC, n=8), model control (MC, n=8), and Rothia  intervention (RG, n=10). Constipation was induced by daily oral  administration of loperamide hydrochloride (10 mg/kg) for 14 days.  The RG group received Rothia mucilaginosa (BNCC363031, 1×10^9 CFU/day)  gavage for 23 days following the modeling period. Fecal samples were  collected on day 44 of the experiment. Gut microbiota composition was  analyzed by sequencing the V3-V4 hypervariable regions of the 16S  rRNA gene using the Illumina platform.  Results: This dataset comprises 16S rRNA gene sequencing data from  26 fecal samples across three experimental groups, enabling  comprehensive analysis of microbiota alterations associated with  constipation and Rothia mucilaginosa intervention.  Relevance: This dataset supports findings from a combined Mendelian  randomization and experimental validation study investigating causal  relationships between gut microbiota and constipation. The data  provides evidence for the protective effects of Rothia mucilaginosa  on gut microbiota composition in constipation.