Single Cell Transcriptome of Highly Heterogenous Hepato-Hematopoietic Lineages in Fetal Liver Organoids from Human Pluripotent Stem Cells
To investigate the differentiation of hematopoietic fetal liver organoids (hFLOs) from induced pluripotent stem cell, we performed single cell RNA-Sequencing on different culture days. Overall design: FLO cultures on day 10 (prior to hematopoietic growth cocktail) and hFLO cultures on days 13, 16 and 20 (after hematopoietic growth cocktai, total n=4 samples) were singularized prior single cell-RNA sequencing