RNA-seq of human gastric cancer cell line AGS treated with Capsaicin against DMSO
Capsaicin has previously been demonstrated to exhibit anti-tumor effect in various cancer type. However, the deep biological function and molecular mechanism of capsaicin was still uncertain. In this research, we used high-throughput RNA sequencing to unveil the potential biological function of capsaicin in human gastric cancer cell line AGS.Total RNA was collected from AGS cells treated with capsaicin (at a dose of 250uM for 24h) or DMSO using TRIzol reagent according to the manufacturer's protocol (n=3 per group). RNA was quantified using a NanoDrop ND-2000 (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA). High throughput sequencing was performed by TsingKe biotech Co., Ltd. according to the manufacturers` standard protocols. The quality control and preliminary analysis of sequencing raw data was performed by Novogene biotech Co., Ltd. according to the standard pipeline. Gene expression level was measured by Fragments Per Kilobase of exon model per Million mapped fragments (FPKM).