Mus musculus strain:C57BL/6J | isolate:feces Metagenome
Objective: To compare the changes in the type and quantity of intestinal flora in the mice from Control group, IBD group, and OPR group, and screen out the intestinal flora that may be related to mechanism of OPR treatment in IBD.Methods: The mouse feces samples were collected from Control group (Healthy mice, n = 4), IBD group (DSS-induced mice, n = 4), and OPR group (IBD mice treated with OPR, n = 4). The genomic DNA of the mouse feces samples was extracted using CTAB, after which the purity and concentration of the DNA was detected using agarose gel electrophoresis. An appropriate amount of the sample was taken in a centrifuge tube, and the sample was diluted using sterile water. The samples were analyzed using the 515F and 806R primers for PCR amplification of the V4 variable region. After amplification and purification, the libraries were constructed using NEB Next Ultra DNA Library Prep Kit library construction kit, and the constructed libraries were detected and quantified by Q-PCR using Agilent 5400, after the libraries were qualified, they were up-sequenced using the Illumina sequencing platform.