Transcriptomics of HEK293T cells expressing AFF4-wild type or AFF4-T829/S831/S833/S834/S835A mutant treated with or without insulin
AFF4 was recently identified as phosphorylated at S831 in response to insulin stimulation. To investigate a potential functional role of this phosphorylation sites, HEK293T cells expressing AFF4-wild type or AFF4-T829/S831/S833/S834/S835A mutant treated with or without insulin for 20 min followed by extraction of total RNA.