rgs1 is a dominant transcriptional suppressor that regulates subset of effectors in M.oryzae during the morphogenesis of appressorium maturation
Many effector genes in M.oryzae are only expressed in plant tissue after invasive growth, and have very low expression in conidia and mycelium. We determined a workflow to screen mutants showing constitutive expression of fluorescence-labeled effector gene fusions in spores. By using quantitative imaging, bioinformatics analysis and genetic complementation, we elucidated that RGS1 (Regulator of G-protein Signalling 1) acts as a dominant transcriptional suppressor and governs a subset of effectors as a regulon in M.oryzae during the morphogenesis of appressorium maturation.