Nanodynamo: studying the dynamics of RNA metabolism through mathematical modelling and native RNA profiling.
The combined action of coordinated nuclear and cytoplasmic steps shapes gene expression programs at steady state and determines their concerted response to perturbations. We developed Nanodynamo, an experimental and computational workflow for the comprehensive quantification of the kinetic rates of nuclear and cytoplasmatic steps of the RNA life cycle. Nanodynamo relies on mathematical modelling following the sequencing of native RNA from cellular fractions and polysomes.