Multi-organ sequencing of diverse age and behavioral trajectory in killifish
This dataset was collected to explore the molecular differences between killifish on long-lived and short-lived aging trajectories. The continuous behavioral tracking system was designed to non-invasively evaluate the aging trajectory of animals while still relatively young, allowing comparison of animals destined for a long lifespan to those destined for a short lifespan even while early in life. We tracked a cohort of animals from adolescence to middle age (150 days old; n=17 animals) at which point we could reliably distinguish long-lifespan vs. short-lifespan trajectories based on behavior for each individual. We then sacrificed animals and performed bulk RNA sequencing of eight different organs (brain, gut, heart, kidney, liver, skin, spleen, and testis) of each individual to investigate molecular signatures of distinct aging trajectories. For context, in addition to evaluating animals on a long- vs. short-lifespan trajectory, we also compared molecular signatures of young vs. old animals. For this measurement, we tracked a cohort of animals up to a relatively young age (80 days; n=8) and a cohort of animals up to advanced age (>=210 days; n=4); both groups were sacrificed followed by bulk RNA sequencing of the eight different organs.