RNA sequencing analysis of lung epithelial cells in conditional SDHD knockout mice and control mice
We ablated the mitochondrial complex II subunit succinate dehydrogenase subunit D gene (Sdhd) in the distal lung epithelium during development by crossing SFTPC-Cre mice with Sdhdfl/fl mice58 (SDHD cKO mice). SDHD is a nuclear-encoded subunit that is essential for the enzymatic activity of mitochondrial complex II. Lung epithelial cells isolated from SDHD cKO mice displayed a decrease in both basal and coupled OCR (Fig. 6b) and an increase in succinate levels compared to those from Sdhdfl/fl mice (SDHD control mice). RNA-seq data revealed that lung epithelial cells from SDHD cKO mice displayed an induction of the ISR.