RNA-seq of astrocytes with CRAC channel perturbation
RNA-seq of cultured primary astrocytes with or without Orai1 KO, in the presence or absence of 0.2uM thapsigargin (TG) + 50nM Phorbol 12,13-dibutyrate (PDBu). Libraries were sequenced on an Illumina NextSeq 500 instrument (Illumina Inc) using NextSeq 500 High Output reagent kit (Illumina Inc) (1x75 cycles) with a target read depth of approximate (5-10) million aligned reads per sample. FASTQ files were generated using bcl2fastq 2.19.1 (Illumina).