RRBS-seq of single-cell clones of four cell Lines reveals highly-conserved methylated sites in DNA
DNA methylation is one of the most studied epigenetics, and its effects on embryonic development, cell differentiation, cell development, aging, apoptosis, carcinogenesis, and genomic stability are all im-portant. In the classical epigenetic model, cytosine methylation is steadily transmitted to daughter cells with fidelity as DNA replicates. However, a large number of studies have shown that there are detectable changes in DNA methylation at many CpG sites during DNA replication. In this study, we selected four different types of cell lines, A549, ARPE-19, Jurkat and SW1353. For a single CpG site in a single cell, there are only three methylation states, 100% methylation, 50% methylation, and 0% methylation, and any DNA methylation that deviates from these three results indicates a methylation change. We used reduced representative bisulfite sequencing (RRBS)  to screen out methylation sites. Overall design: RRBS-seq combined with RNA-seq analysis to find high-fidelity methylation CpG sites in four kinds of cell lines