Gene expression profile of Fcrls+/CD11b+/Ly6C- microglia from the brain V
We used bulk RNA sequencing to investigate the molecular signature of microglia affected by deletion of microglial APOE4 in APP/PS1 mice. Overall design: Fcrls+/CD11b+/Ly6C- microglia were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using Smartseq2 RNAseq.