Loss of starvation and light sporulation trigger in Myxomycetes Physarum roseum
We sequenced plasmodia of the slime mould Physarum roseum to test whether the canonical starvation plus light trigger for sporulation, well established in Physarum polycephalum, is conserved in this species.Two sequencing sets were generated:(1) NovaSeq X Plus, paired-end 150 bp: one library for each of two treatment combinations, dark plus nutrient rich (DN) and light plus starvation (LS), yielding four FASTQ files (R1 and R2 for each condition). These reads were pooled for de novo transcriptome assembly.(2) NextSeq 500, single-end 75 bp: RNA-Seq libraries for the complete 2 x 2 factorial design of illumination (dark vs light) and nutrition (rich vs starvation). All four unique combinations (DN, DS, LN, LS) were prepared in triplicate (n = 3), producing 12 FASTQ files for differential gene expression analysis.All libraries were poly(A)-selected.