Genome-wide identification of target genes for the transcription factor Sox10 in the Msc80 Schwann cell line.
To identify target genes of the Sox10 transcription factor in peripheral glia in a genome-wide and unbiased approach, we performed CRISPR/Cas9-dependent genome editing of the mouse Sox10 gene in the Msc80 Schwann cell line, followed by RNA-Seq studies on Sox10-expressing control Msc80 cells and Sox10-negative genome edited cell clones. Among others we detected substantial changes in the expression of genes associated with Schwann cell adhesion and the formation of the node of Ranvier. Overall design: We generated mRNA profiles of Msc80 cells (ctrl) and Sox10-negative Msc80 cells (Sox10ko) using the Illumina HiSeq 2500 platform.