2bRAD-M analysis of the urinary microbiota in patients with calcium oxalate kidney stones
In this pilot cross-sectional study, we used 2bRAD sequencing for microbiome profiling and liquid chromatography-mass spectrometry-based metabolomics to characterize urinary microbial and metabolic features. We analyzed urine samples from a pilot cohort of 12 patients with calcium oxalate kidney stones and 10 healthy controls. Statistical analyses of microbial diversity and metabolomic profiles were conducted to explore between-group differences. To explore microbiome-metabolite associations, we performed Spearman correlation analysis with multiple-testing correction and provided stratified correlation heatmaps as supplementary analyses.