Gut Microbiome Profiling in Wild-Type and Complement-Deficient Mice (C3-/-, C4-/-) Using 16S rRNA Sequencing
This project investigates gut microbiome differences among wild-type (WT) mice and complement component deficient models (C3-/- and C4-/-). Microbial communities were characterized through 16S rRNA gene sequencing, targeting the V4 hypervariable region. Amplicons were sequenced on the Illumina MiSeq platform, enabling comparative analysis of microbial composition and diversity across genotypes.