Candida albicans Dal81and Stp2 regulated genes under pH alkalization
"SD1," "SD3," and "SD4" represent three biological replicates of Candida albicans SC5314 cultured in synthetic dextrose minimal (SD) medium for 6 hours. "YNB1," "YNB2," and "YNB3" denote three biological replicates of SC5314 cultured in YNB + 1% CAA medium (with an initial pH of 4.5) for 6 hours. Total RNA was extracted from these six samples, after which RNA-seq libraries were constructed. Subsequently, the cDNA was sequenced using an Illumina HiSeq 2000 genome analyzer (Novogene Company, Beijing, China)."dal81ko_1," "dal81ko_2," and "dal81ko_3" represented three biological replicates of the dal81ko strain. "stp2ko_1," "stp2ko_2," and "stp2ko_3" denoted three biological replicates of stp2ko strain. "Wt_1," "wt_2," and "wt_3" stood for three biological replicates of WT strain. All nine fungal strains were cultured in YNB + 1% CAA medium (with an initial pH of 4.5) to log phase. Total RNA was extracted from each sample and RNA-seq libraries were constructed and cDNA was sequenced on an Illumina HiSeq 2000 genome analyzer (Novogene Company, Beijing, China)."H1" and "H2" represent two biological replicates of immunoprecipitated chromatin from Dal81-13xMyc strains, while "H3" denotes the total chromatin from SN250 as the untagged control."H4" and "H5" are two biological replicates of two biologic repeats of immunoprecipitated chromatin from Stp2-3xHA strains, with "H6" being the total chromatin from SN250 as the untagged control. All the fungal strains were cultured in YNB + 1% CAA medium (with an initial pH of 4.5) to log phase. DNA samples from these six groups were used to construct the sequencing library by Novogene Company (Beijing, China). Then, two paired end sequence of approximately 150 base pairs (bp) was performed using an Illumina HiSeq 2500 genome analyzer (Beijing Novogene Co. Ltd).