RNA-Seq results of oligodendrocyte model of SBMA, AR-97Q cells, compared to AR-17Q cells
Transcriptional changes of AR-97Q cells and AR-17Q cells were compared. RNA-seq gene expression analysis revealed that differentially expressed genes were associated with regulation of signaling and regulation of cell communication as biological process, and membrane was most relevant to the genes as cellular component. Overall design: Lentivirus expressing full-length human AR containing 17 or 97 CAG repeats were introduced into Oli-neu cell, thereby generating cell lines harboring AR-17Q as control cells and cell lines harboring AR-97Q as a cellular model of SBMA. Differentiation was induced by adding 1 µM PD174265 (Calbiochem) to the medium for 72 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 samples from each cell line.