FADU RNAseq 7-day decitabine treatment
Stranded 150 bp paired-end RNA-sequencing of FaDu (ATCC, HTB-43) head and neck squamous cell carcinoma (HNSCC) cells. Cells were treated for 7 days with either vehicle control (DMSO) or 1 micromolar decitabine, a DNA methyltransferase inhibitor, in biological triplicate. Decitabine treatment induces widespread transposable element (TE) demethylation and reactivation alongside complex gene-level expression changes. This dataset was generated to serve as a biologically relevant benchmark for evaluating RNA-seq quantification tools, specifically testing their ability to accurately resolve and probabilistically separate overlapping transcriptional signals from protein-coding genes and locus-level transposable elements.