Global transcriptomic profiling of PTI- and ETI-responsive genes in wild-type Col-0 and mutant rh3 Arabidopsis thaliana plants
Plants utilize a two-tiered immune system, called pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), to recognise and defend against pathogens. PTI acts as the first line of defense, limiting pathogen invasion, whereas ETI provides a stronger response that potentiates PTI signaling. Here, we performed a global transcriptome analysis to compare immune-induced gene expression during PTI and ETI in wild-type Col-0 and the rh3 mutant Arabidopsis thaliana plants. Adult leaves were inoculated with a mock solution (MgCl2), the non-pathogenic strain Pseudomonas syringae pv. tomato (Pst) DC3000 hrcC?, or the effector-triggering strain Pst DC3000 (avrHopZ1a). Leaf samples were collected 8 hours post-inoculation, with three independent biological replicates performed per condition.