Mesenchymal cell-derived septoclasts resorb cartilage during endochondral ossification and fracture healing
For scRNA-seq of fracture bone , 14 days post-fracture (PFD14) femur and control bones were harvested, cleaned surrounding tissue and the nail insert was removed from fracture bone. Control and PFD14 bone single cell suspension and non-hematopoietic stromal cells were prepared for scRNA-seq . Overall design: Fracture bone single cell -RNA sequencing of non-hematopoietic  bone stromal cells (BSC)  using  10X genomics