RNA-seq of THP-1-derived macrophages with or without INSIG1 knockdown
THP-1 monocytes carrying either control shRNA (shCON) or INSIG1-targeting shRNA (shINSIG1) were differentiated into macrophages by 100 nM PMA for 24 h, followed by a 12 h rest. Total RNA from three independent biological replicates was subjected to Illumina NovaSeq X Plus paired-end RNA-seq to identify genes regulated by INSIG1 during macrophage differentiation.