Identify and characterize the polyploidization process of primary human proximal tubular cells (hPTC) [scRNA-seq]
We generated single-cell RNA sequencing (scRNAseq) datasets of primary cultures of  human proximal tubular cells stimulated with TGF-ß1 or vehicle. Following TGF-ß1 stimulation, hPTC expressed TGF-ß1 and its downstream targets. Moreover, TGF-ß1-treated hPTC were characterized by a differential expression of pro-fibrotic genes, which we had recently showed to be characteristic of polyploid hPTC and were enriched with hypertrophy, indicative of cell polyploidization. Overall design: Analyze the role of  TGF-ß1 in promoting polyploidization in primary human proximal tubular cells (hPTC)