Transcript degradation profiling for Mycolicibacterium smegmatis in log phase, hypoxia, and RNase E knock down conditions.
We calculated transcriptome-wide mRNA half-life using RNAseq to characterize the mRNA stability in log phase and hypoxia, as well as the impact of RNase E on mRNA degradation. Overall design: mRNA abundance were measured at multiple time points after rifampicin treatment using Mycolicibacterium smegmatis in log phase, hypoxia, and RNase E knockdown condition. Three RNAseq replicates were used for each time point.