RNAseq profiling of eds1 and pad4 mutants inoculated by fungus Alternaria alternata in Arabidopsis
To explore the role of plant hormone salicylic acid (SA) during disease development of Arabidopsis, RNAseq was conducted to analyse the expression pattern in eds1 and pad4 mutant plants after inoculation of necrotrophic Alternaria alternata. Methods: RNAseq libraries were sequenced on an Illumina Hiseq X Ten plantform with standard Illumina protocols. Results: Loss-function of EDS1 or PAD4 in Arabidopsis led to higher expression level of 1O2 response genes induced by A. alternata than Col-0. Overall design: at least three 3-wk-old eds1 and pad4 mutant plants, inoculated with 3-mm-PDA plugs containing A. alternata actively mycelia or 3-mm-PDA plugs without mycelia in vivo. After 12 h treatment, two rosette leaves per plant were sampled and stored immediately in liquid nitrogen. Three independent experiments were done. Data were analysed on the basis of treated vs untreated comparison.