CRISPR screens reveal regulators of chemoattractant receptor FPR1 trafficking
We completed genome-wide CRISPR screens to identify genes controlling the cell surface levels of the chemoattractant receptor FPR1 in a cell line model for human neutrophils. We used deep sequencing to analyze the results of the screens. We identified genes affecting FPR1 biogenesis, secretion, endocytosis, and recycling. We also used amplicon sequencing to confirm mutation of several specific genes.