Fast and robust characterization of RNA targets of RNA-binding proteins by RNA-editing strategy coupled with machine learning in human embryonic stem cells
RNA-binding proteins (RBPs) play a key role in regulating the maintenance and differentiation of embryonic stem cells. A critical step in understanding RBP function is to identify the RNA transcripts to which it binds. To achieve this goal, we developed an induction strategy named RNA-Editing-Based-RNA-seq (REB-seq) to capture potential RNA targets bound by RBPs of interest. To achieve this goal, we constructed cells overexpressing IGF2BP1, IGF2BP2, and IGF2BP3 in hESCs and transferred them into ADAR, hAPOBEC, and rAPOBEC