The Role of Satb1 in Shaping the Repertoire of T Cell Receptor Genes
To explore the role of Satb1 in rearrangement and repertoire of TCR genes, we used Satb1contional knockout micwhich the Satb1 gene was deleted in long term hematopoietic stem cells. We applied 5'RACE (rapid amplification of cDNA ends) PCR technique to generate deep sequencing libraries of Tcrd, Tcrg, Tcrb, and Tcra cDNA by using a single set of primers for each TCR gene. We obtained millions of TCR sequences and unraveled the complexity of T-cell diversity. Our experimental evidence demonstrates that Satb1 has substantial influences on the TCR repertoire. Overall design: TCR repertoire profiles of 4-12 weeks old wild type (WT) and Satb1cKO mice were generated by deep sequencing, in trplicate, using Illumina Hiseq