Epistasis between drug resistance-conferring mutations in Mycobacterium tuberculosis
To analysze epistatic interactions between high-level RpoB and GyrA drug resistance mutations in Mycobacterium tuberculosis, starting from nine genetically distinct drug susceptible isolates of MTBC L1, L2 and L4, we picked single- and double-drug resistant mutants carrying RpoB and or GyrA drug resistance mutations alone and in combination.