Abiotic Treatment to Common Bean Plants Results in an Altered Endophytic Seed Microbiome
Common bean plants (Phaseolus vulgaris L. (var. Redhawk)) were grown in an environmental growth chamber under 3 different growth conditions: control plants with ample water provided, water-withholding plants given 66% less water, and nutrient-addition plants given 1/2X Hoagland solution (nutrient solution) in place of water. Amplicon sequencing of the V4 region of the 16s rRNA gene and the ITS gene of the seed endophytic DNA from each condition was performed using the Illumina MiSeq platform to assess seed bacterial/archaeal and fungal community structure, respectively. Code for analysis is available on GitHub: https://github.com/ShadeLab/BioRxiv_Seed_Microbiome_2020/blob/master/README.md