OTAR3083_Project_Symphony_10X
Pilot screen for Cas13d targeting coupled with 10X single cell gene expression readout. The guide RNA library consists of 12 guideRNAs targeting 3 genes plus 3 controls. Screen conducted in two cell lines. the sequencing pool contains 4 indexed gene expression libraries and 4 indexed guideRNA libraries, pooled together and sequenced in one lane of Novaseq S4.