Complex long and short read reanalysis of the transcriptome of VZV
The Herpesvirus genomes, like other viral genomes consist of complex, gene-dense structures, which can be explained by the parsimonious evolution of viruses, which also enables their high coding capacity. For SRS is difficult, unless it is unable to distinguish these complex transcriptomic structures, however LRS can detect length isoforms of mRNAs very well, especially in overlapping transcriptomic regions. Albeit, many newly found transcript isoforms of Herpesviruses remained unevaluated, due to the low level of abundance, or uncertain detection of transcriptional start and end positions. Therefore, the reanalysis of transcriptomic data is in demand. A new integrative elaboration of SRS and LRS transcriptomic analysis of transcriptomic data can resolve the hiatus of Herpesvirus transcriptome annotation.