H3K4me1 ChIP-Seq experiment using cells from stage 15 Drosophila melanogaster embryos sorted by reporter gene activity
The aim of this study was to analyze if transcriptionally active svb loci show enrichment for H3K4me1. To capture cells where svb-related ventral enhancers are active, we FACS-sorted nuclei from stage 15 Drosophila melanogaster embryos based on the expression of different reporter genes driven by different svb enhancers. The svb_E10 enhancer drives the expression of GFP, whilst dsRed is driven by the svb_7 enhancer. The sorted nuclei were then used for ChIP with anti-H3K4me1 and anti-H3 (for normalization purposes) antibodies.