Ambrosia artemisiifolia endobacteriome
Amplicon-based (16S rRNA gene, V3-V4 region) characterization of the endophytic bacterial communities associated with Ambrosia artemisiifolia L. (common ragweed) across six sites in Central Europe. The sampling design comprises two invasion fronts - Podkarpackie Voivodeship (SE Poland) and Bratislavsky kraj (SW Slovakia) - each represented by a pair of sites: an agricultural habitat (maize field) and a dry meadow located ca. 500 m apart. Two additional roadside sites in Upper Silesia (S Poland) serve as references. The study aims to characterize the core endobacteriome of leaves, roots, and seeds and to assess its variation across habitat types and geographic locations.