Effect of propranolol on murine B16F10 metastasis-bearing lungs or on monocytes isolated from these lungs
We demonstrate that the ß-blocker propranolol strongly reduces lung metastasis in multiple murine models, with the reduction being completely dependent on CD4+ T cells. To dissect the underlying mechanisms behind the anti-metastatic effect of propranolol, an experimental B16F10 lung metastasis model was combined with propranolol treatment. Total lung RNA or RNA from FACS-isolated lung monocytes and magnetic bead-isolated CD4+ T-cells were subjected to RNA sequencing. Overall design: C57BL/6 mice were i.v. injected with B16F10 melanoma cells leading to the formation of lung metastasis. Total lung RNA or RNA from FACS-isolated lung monocytes and magnetic bead-isolated CD4+ T-cells were subjected to RNA sequencing.