DGCR8 promotes intestinal regeneration of damaged epithelium in a p53 signaling dependent manner
TO explore role of DGCR8, a essential factor in canonical microRNAs biogenesis, in intestinal epithelial regeneration, we performed gene expression profiling analysis using data obtained from RNA-seq of intestinal organoids, intesitnal stem cells, and crypts from mice following 5-FU administration. To further investigate which microRNA was responsible for intestinal regeneration, we also performed small RNA sequencing with intestinal stem cells and intestinal epithelia cells. Overall design: Comparative transcriptome profiling analysis of RNA-seq data for intestinal stem cells, intestinal organoids, and crypts from mice following 5-FU administration.