RIP-Seq analysis of non-PPR editing factors OZ1, ORRM1, RIP9/MORF9
We provide sequences of maize chlroplast RNAs associated with non-PPR editing factors through immunoprecipitation. The accumulated sequences indicate expansive role in RNA processing and potential associations of OZ1-ORRM1 complexes with the ribosome and less efficiently translated mRNAs. Overall design: Three specific purified polyclonal antibodies were bound to magnetic protein A/G beads. Beads were then suspended in maize chloroplast extracts and RNAs isolated and sequenced after washing.