Time-course transcriptome analysis of TF-induced iPSC neurons
We report on a comprehensive time-course study of transcription factor (TF)-induced iPSC neurons cultured in vitro through an intrinsic maturation program following neurogenesis. Combining the present RNA-seq study with immunocytochemistry and electrophysiological analyses, we determined the transcriptional and morphological sequences of key developmental events associated with postnatal brain-like transition and spinogenesis. TF-induced iPSC neurons successfully acquired structural and functional synaptic maturity, which will critically expand their utility in modeling higher brain functions and disorders. Overall design: Comparative gene expression profiling analysis by RNA-Seq of TF-induced iPSC-derived neurons at day 0, 10, 32, 57, and 88 of culture after induction of differentiation.