16S rRNA sequencing of root nodules and rhizosphere soils of three Alnus spp.
The V3-V4 region of the 16S rRNA gene was amplified using the 338F: 5'-CCTACGGGNGGCWGCAG -3' and 806R: 5'- GACTACHVGGGTATCTAATCC -3' universal primer set from the genomic DNA extracted from root nodules and rhizosphere soils of three Alnus spp.