Saccharum hybrid cultivar ROC22 Transcriptome or Gene expression
we established a homologous suspension cell line from the drought-tolerant cultivar 'Roc22'. A gradient of polyethylene glycol 6000 (PEG-6000)-induced drought stress (0%, 5%, 10%, 20%) was applied, enabling integrated cytological, physiological, and transcriptomic analysis.This study provides the first systematic dissection of a multi-dimensional drought response network in sugarcane at the cellular level, integrating water regulation, oxidative defense, cell wall remodeling, and cell cycle modules. Our findings offer deep mechanistic insights and establish a novel "cell model + multi-omics" paradigm for crop stress biology research.