Rattus norvegicus Genome sequencing and assembly
Background and Rationale:Traumatic brain injury (TBI) is a leading cause of long-term disability, often resulting in a comatose state characterized by widespread cortical suppression. Combined central (e.g., rTMS) and peripheral (e.g., Press Tack Needle) neuromodulation has shown promising synergistic effects in promoting arousal, but the underlying molecular mechanisms remain largely unknown. This study was designed to uncover the transcriptomic changes in the prefrontal cortex (PFC) that mediate the therapeutic effects of this combined intervention, with the goal of identifying novel therapeutic targets for TBI-induced coma.Experimental Design:This submission includes transcriptome data from three key experimental groups (n=3 per group) of adult male Sprague-Dawley rats. A severe TBI-induced coma model was established using the controlled cortical impact (CCI) method. The groups are as follows:Sham group: Rats underwent craniotomy without the cortical impact injury.TBI group: Rats received the TBI procedure but no subsequent therapeutic intervention.rTMS+PTN group: TBI rats were treated with a synergistic combination of 20 Hz repetitive transcranial magnetic stimulation (rTMS) and Press Tack Needle (PTN) stimulation.Methods and Data Processing:Prefrontal cortex (PFC) tissue was collected 24 hours post-injury. Total RNA was extracted, and mRNA sequencing libraries were prepared and sequenced on an Illumina NovaSeq platform. The generated raw data were processed to identify differentially expressed genes (DEGs), which were subsequently used for pathway enrichment analysis, protein-protein interaction (PPI) network construction, and Gene Set Enrichment Analysis (GSEA). The primary goal of this analysis was to identify the key signaling pathways that are dysregulated after TBI and restored by the combined neuromodulation therapy, ultimately leading to the identification of the Egr1->NTSR1->Drd1 axis as a central mechanism.This dataset provides a valuable resource for researchers studying the molecular pathophysiology of TBI-induced coma and the mechanisms of synergistic neuromodulation therapies.