Gene expression analysis of TGFß1-stimulated fibroblasts to assess anti-fibrotic effects of the bisubstrate-like NNMT inhibitor II559
Fibroblast activation drives tissue fibrosis and is often mediated by TGFß1 signaling. Human fibroblasts were pre-treated with the NNMT inhibitor II559 for 12 hours, followed by TGFß1 stimulation for 72 hours. Transcriptomic profiling revealed that II559 prevented pro-fibrotic gene expression, providing a resource for understanding how NNMT inhibition modulates fibroblast activation and fibrotic remodeling. Overall design: Human dermal fibroblasts(n=3) were treated with TGF-ß1 (10 ng/mL) in the presence or absence of II559 (30 µM). Following 72 h incubation, RNA was harvested and subjected to RNA-seq.