Transcriptome profiling of pitp-1 mutant and pitp-1(RNAi)-treated Caenorhabditis elegans
This dataset contains the transcriptome profiling results of Caenorhabditis elegans under pitp-1 regulation. The experiment was designed to identify the transcriptional mechanisms underlying the pitp-1 suppression-mediated lifespan extension. Transcriptomic analysis was performed on pitp-1 mutant and pitp-1(RNAi)-treated worms at Day 3 of Adulthood (D3A). Bioinformatic pathway analysis (IPA) of the resulting differential expression profiles revealed a significant and coordinated downregulation of genes associated with the IIS (Insulin/IGF-1 Signaling) and TOR signaling pathways. This dataset therefore provides key molecular insight into how PITP-1 transcriptionally affects the major longevity regulatory networks. Overall design: Transcriptome profiling was performed on D3A worms with two biological replicates for each condition. Samples included pitp-1 mutant (pitp-1(pe1297) and its wild-type control (N2), as well as pitp-1(RNAi)-treated N2 worms and their Empty Vector(EV) control.