Full-length transcriptome sequencing for the stylo root tips
Since the genome sequence information for stylo is not well available, the full-length transcriptome sequencing of stylo genotype RY#2 was conducted using the root tips treated with and without Al. Results showed that the length of the open reading frame (ORF) of most transcripts detected was below 2000 bp, with an average length of 962 bp, minimum length of 297 bp, and longest length of 7963 bp. The predicted ORF were annotated according to the BLASTP, NR, NT, and BLASTX databases, and resulted in 20819, 35143, 30659, and 27769 genes with functional annotation, respectively. A total of 18891 genes were functionally annotated in all of the four databases