Copper oxide nanoparticles treated MH-S cells
The three groups of MH-S cells were control group, 20 µg/ml CuO NPs group, and 20 µg/ml CuO NPs + 10 µM TTM group. Total RNA of the  MH-S cells were extracted using cell RNA isolation kit (Vazyme Biotech Co., Ltd, China). The number of MH-S cell sample for each group were three (n = 3 cell samples/group). All samples were sent to Majorbio Biotech (Shanghai, China) for transcriptome sequencing. Overall design: The three groups of MH-S cells were control group, 20 µg/ml CuO NPs group, and 20 µg/ml CuO NPs + 10 µM TTM group for 24 h.