Oxford Nanopore cDNA sequencing of Pleurodeles waltl thymi
Various immune genes were absent from the Pleurodeles waltl genome. We suspected these were due to lack of RNA sequencing data. To overcome this lack of immune-specific RNA sequencing data and thus augment gene annotations in the P. waltl genome we performed Oxford Nanopore cDNA sequencing on thymi. We identified various immune transcripts that were unannotated in the P. waltl genome assembly.