RNA-seq analysis of liver sinusoidal endothelial cells exposed to hydrostatic pressure stimulation
Primary murine LSECs (mLSECs) were exposed to hydrostatic pressure (HP) stimulation. The expression of YAP/TAZ target genes such as CTGF and basement membrane-related genes such as COL4A1was upregulated in mLSECs exposed to HP stimulation. Overall design: After incubation on a Collagen I-coated plate overnight, the cells were serum-starved for 6 hours and then exposed to periodic HP stimulation for repeated cycles (0.01 Hz) between 101 and 135 kPa (absolute) for 8 hours using our original HPS system. The system was maintained at 37°C in a CO2 incubator (5% CO2).