Metagenomics of intestinal microbiomes from mice fed different sources of dietary protein
This project contains shotgun metagenomes generated from pooled samples for the purpose of creating a protein database for metaproteomics. We fed conventional C57BL/6J mice a series of defined diets where the source of dietary protein was the only difference, which made up twenty or forty percent of the diet. The dietary protein sources used were purified protein. The diets were fed to 12 mice across four cages for one week each. A fecal sample was collected at the end of each week. The diets were fed in this order: standard chow, 20% soy, 20% casein, 20% rice, 40% soy, 20% yeast, 40% casein, 20% pea, 20% egg white protein, 20% chicken bone broth, and lastly at the end of the experiment half of the mice were fed the 20% soy and half the mice the 20% casein diet again as a control. We pooled samples by cage from the 20% rice diet, 40% soy diet, 20% yeast diet, 40% casein diet to generate four metagenomes, one for each cage. The proteomes that used a database generated from this study were submitted to PRIDE: PXD041586 and PXD04064.