Transcriptomic Data Reveals MYC as an Upstream Transcriptional Regulator in Laying Hen Pre-ovulatory Follicles using Ingenuity Pathway Analysis
To investigate the difference between the pre-ovulatory F5 and pre-recruitment follicular stages in laying hens and identify master regulators that control the processes in these two follicular stages. RNA was isolated from a group of 3-5 pre-recruitment follicles and the most recently recruited pre-ovulatory follicle (F5). After RNA isolation, follicles underwent RNA sequencing and differential gene expression analysis occurred. Overall design: Follicles from two follicular stages from 12 month old hens were used. Total RNA was obtained folliclular tissue samples using TRIZol reagent and RNA-Sequencing occurred.