Idnetification of a new HLA-C*04 allele a french  voluntary bone marrow donor
Here we describe a novel C* 04 allele, identified in  a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology. Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  A*02:01,*03:01 B*39:01, *44:03 C*04:New ,*15:02 DRB1*03:01,*07:01 DRB3* 01:01DRB4* 01:01 DQA1 *02:01,*05:01 DQB1* 02:01,*02:02 DPA1* 02:01, *- DPB1* 01:01,*10:01 HLA-C*04 : new allele differs from C*04:100 by one nucleotide substitution at position 76 of exon 3 (position 419 of cDNA), where  base T is replaced by A , inducing F instead  Y (116TTC>TAC).