Cellular heterogeneity and dynamics of the human uterus in healthy premenopausal women
We applied single-cell RNA sequencing to profile >50K uterine cells from both the endometrium and myometrium of 5 healthy premenopausal individuals, and jointly analyzed the data with a previously published dataset from 15 subjects. The resulting normal uterus cell atlas contains >167K cells representing the lymphatic endothelium, blood endothelium, stromal, ciliated epithelium, unciliated epithelium, and immune cell populations. Focused analyses within each major cell type and comparisons with subtype labels from prior studies allowed us to document supporting evidence, resolve naming conflicts, and to propose a consensus annotation system of 39 subtypes. We find many subtypes show dynamic change over different phases of the cycle, and identify multiple potential progenitor cells at multiple levels: compartment-wide progenitors for each major cell type, transitional cells that are upstream of other subtypes, and potential cross-lineage multipotent stromal progenitors that may be capable of replenishing the epithelial, stromal, and endothelial compartments. When compared to the healthy premenopausal samples, a postpartum and a postmenopausal uterus sample revealed substantially altered tissue composition, involving the rise or fall of stromal, endothelial, and immune cells. The cell taxonomy and molecular markers we report here are expected to inform studies of both basic biology of uterus function and its disorders. Overall design: Creation of cell atlas: Characterizing cellular composition and heterogeneity of healthy premenopausal, postmenopausal, and postpartum human uterus using 10x scRNA-seq analysis