A spatial temporal RNA-seq of Fusarium graminearum during symptomless and symptomatic wheat infection
RNA-sequencing of  F. graminearum parental PH-1 during the establishment and progression of wheat infection (cultivar Bobwhite) at 3 and 7 days post inoculation (dpi). At anthesis, 5 µl droplets of 5x10^4 conidia were placed in the floral cavity of the outer florets of the 5th and 6th spikelet from the top of the wheat head. The inoculated plants were kept at high humidity for 48 h, of which the first 24 h were dark  The inoculated wheat spikelets (SP) and sequential rachis segments below the point of inoculation were harvested. Pairs of rachis segments, which were phenotypically similar in the parental PH-1 infection, were combined, representing the transcriptionally distinct fully symptomatic (R1-2), early symptomatic (R3-4) and late symptomless (R5-6) and early symptomless (R7-8) infection phases.   Data was published and analysed in Dilks et al., (2019). Non-canonical fungal G-protein coupled receptors promote Fusarium head blight on wheat. PLOS Pathogens. https://doi.org/10.1371/journal.ppat.1007666