Single-nucleus RNA sequencing of skeletal muscle development in broilers (PRJCA044373)
Muscle is essential for maintaining basic physiological functions in vertebrates and is also an important source of protein. However, as a highly heterogeneous tissue, the cell types and transcriptional changes at the single-cell level during postnatal  development, particularly in poultry, remain largely unknown. To characterize cellular heterogeneity and transcriptional signatures during poultry muscle development, single-nucleus RNA sequencing (snRNA-seq) on pectoral muscle from 2-day-old and 42-day-old Arbor Acres white-feather broiler was performed in this study. This analysis robustly classified myogenic and non-myogenic populations and revealed dynamic shifts in cellular composition during development.