RNAseq of CD4 T cells from 83 individuals with musculoskeletal compains
RNAseq of CD4 cells from 83 individuals with musculoskeletal complains (age 51y, range 19-86. male 22%) were collected at the Rheumatology Clinic during 2022-2023 . Cells were activated with anti-CD3 for 48 hours. Overall design: CD4 cells were isolated by positive selection on Dynabeads (cat.11331D) from human PBMC. Cells were stimulated with anti-CD3 (OKT3, Sigma-Aldrich)  for 48h