Transcriptomic analysis of Babesia infection in the midgut of ticks
The enriched RNA was fragmented and reverse-transcribed into cDNA using random hexamers. Second-strand cDNA was synthesized, purified, and used to construct sequencing libraries through end repair, A-tailing, and adapter ligation. Strand-specificity was achieved by incorporating dUTP during second-strand synthesis and subsequent degradation with Uracil-N-Glycosylase (UNG)