NIPBL-mediated RAD21 facilitates tumorigenicity by the PI3K pathway in non-small-cell lung cancer
After the extraction of total RNA from H1299 and H1650 cells by TRIzol (Invitrogen),cDNA libraries were constructed and sequenced using Illumina NextSeq 500. Cleanreads (clean data) with high quality were obtained, and the levels of mRNAs werecalculated to identify differentially expressed genes (DEGs) in RAD21-silenced cellscompared to control cells