ChIP_seq of AtGTL1 after immune elicitation
Purpose: The goal of this study is to demonstrate the target genes of Arabidopsis transfactor GTL1 in response to immune elicitation.Methods: Four-week-old Arabidopsis XVE-GTL1-HA plant leaves were sprayed with estrodial to induce GTL1 expression for 16 hours and sprayed with flg22 and NaSA to activate immune signalling harvested at 4h. Purified DNA by ChIP methods was deep sequencing by Illumina. Raw data were cleaned up and trimmed and reads were mapped to Arabidopsis genome to get the target genes.Results and Conclusions: This analysis revealed widespread GTL1 binding across the plant genome. And ChIP-seq peak tracks revealed GTL1 occupancy at many key immune loci.