PvWRI1 Genome sequencing of Plukenetia volubilis
WRI1 is a class of transcription factors containing an APETALA2 domain, which is essential for the oil biosynthesis. In this study, the genomic sequence of PvWRI1 was cloned from fresh leaves of Plukenetia volubilis and analyzed by bioinformatics methods. By constructing an overexpression vector, PvWRI1 gene was transformed into tobacco to obtain transgenic positive tobacco plants. Wild-type and transgenic mature seeds were harvested and the fatty acid components were determined by gas chromatography. The DNA sequence of PvWRI1 was 5072 bp, the coding region is 1312 bp, encoding 436 amino acids, and including 7 exons and 6 introns. Analysis of the protein's hydrophobicity revealed that PvWRI1 was hydrophilic. Domain analysis showed that the protein contained two AP2/ERRP structural regions and four inherent disordered protein regions, and the secondary structure analysis showed that the protein was relatively conservative. Phosphorylation predicts the characteristics of N-terminal chloroplast transport peptides. Amino acid homology analysis showed that the AP2 domain had high homology in several species, and the phylogenetic tree showed that PvWRI1 had the highest homology with WRI1 of castor. According to the analysis of fatty acid components, the contents of stearic acid and linolenic acid increased significantly, increasing by 16.8% and 7.5%, respectively, while there was no significant difference among the contents of palmitic acid, oleic acid and linoleic acid. The total oil content increased from 12.84% to 24.06%, and it was up to 1.87 times. At the same time, the linoleic acid / linolenic acid balance ratio decreased by 7.8%. As a crucial regulator in fatty acid biosynthesis, PvWRI1 plays a guiding effect on improving oil content and fatty acid balance in oil plants.