Targeted sequencing of Ripk1 mouse gene treated with AAV-SaCas9
To assess the role of RIPK1 in HDV-mediated liver injury, we developed a CRISPR/Cas9 gene editing system that specifically knockdown RIPK1 expression in hepatocytes. We achieve this by expressing the Cas9 protein under the control of a liver specific promoter that was delivered using a rAAV vector with hepatic tropism.