scRNASeq of Na?ve, GC, plasma, memory B cells sorted from WT and Fip200-deficient mice spleen
We have employed a scRANSeq to study the difference between WT and Fip200-deficient B cell populations from mice spleen 11 days after immunization. FIP200 plays an important role in regulating LC3-dependent and LC3-independent autophage pathway, which is crucial for B cell development and differentiation. This study presents the transcriptome of Naive, GC, plasma and memory B cell populations at the peak of mice immune-response and provides new insights into the role of FIP200 in regulating plasma differentiation. FIP200f/fMb1Cre+/- (KO) mice and their littermatecontrol, FIP200f/fMb1Cre-/-(WT) were immunized with 50 ug NP29-KLH with Alum, then splenocytes isolated from WT and KO mice 11 days after immunization were enriched for GC, plasma, and memory B cells by depleting non-B cells and IgDhi B cells using magnetic beads. Those enriched samples were then barcoded and sorted for na?ve, GC, plasma, and memory B cells, which were then mixed for single-cell RNA sequencing.