Human osteoarthritic articular cartilage stem cells suppressed osteoclasts and improved subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3
Though articular cartilage stem cells (ACSCs) based therapies have been demonstrated to be a promising option in the treatment of diseased joints, the wide variety of cell isolation, the unknown therapeutic targets, and the incomplete understanding of the interactions of ACSCs and diseased microenvironments have limited the applications of ACSCs. The ACSCs treated with or without 20 ng/mL human TNF-a, the high-throughput RNA sequencing of TNF-a primed ACSCs was performed. Bioinformatic and functional analysis showed that ACSCs suppressed osteoarthritic osteoclastogenesis, and inflammatory joint microenvironments augmented the inhibitory effects.