Next Generation Sequencing Facilitates Quantitative Analysis of Lactic acid Treated Human Treg cells Transcriptomes
RNA-seq of Treg cells sorted from peripheral blood mononuclear cells (PBMC) and treated with or without lactic acid. Overall design: we polarized CD4+ T cells from PBMC into inducible Tregs (iTregs) in vitro and treated them with or without 10 mM lactic acid for 24 hours.