Genotyping-by-sequencing based identification of fertility restorer locus in F2 population
An F2 population derived from a cross between a cytoplasmic male sterile line (IIHR4392-A) and a fertility restorer line (IIHR4597) was used to identify genomic regions associated with restoration of fertility. Genotyping-by-sequencing (GBS) was performed on selected F2 individuals and parents to generate SNP markers for bulk segregant analysis.