Analysis of gene regulation by the TP63 isoforms deltaNp63alpha and deltaNp63beta
We analyzed the transcriptome changes of the colon carcinoma cell line HCT116 TP53-/- in response to expression of the TP63 isoforms deltaNp63alpha or deltaNp63beta. The goal was to understand how these C-terminal isoforms of p63 might differentially regulate gene expression. Overall design: PolyA+ RNAseq (in biological triplicate) of HCT116 TP53-/- cell lines expressing either human deltaNp63alpha or deltaNp63beta in uninduced (- doxycyline) or induced (+ doxycycline) conditions for 24hrs.