16S rRNA sequencing of colonic contents of TG pigs
Here we successfully constructed a transgenic (TG) Bama pig model using CRISPR/Cas9 technique by knocking in three humanized risk susceptibility genes. TG pigs were also administered a high-fat and high-sugar diet (HFHSD) for 4, 8, and 12 months to accelerate the progression of metabolic disorders. The colon microbiome profiles of TG pigs were characterized using 16S rRNA.