Effect of combination treatment of RNA polymerase I transcription inhibitor CX-5461 and histone deacetylase inhibitor panobinostat on gene expression in human multiple myeloma cell lines MM.1S and JJN3
The high rates of protein synthesis and processing render multiple myeloma (MM) cells vulnerable to perturbations in protein homeostasis. The induction of proteotoxic stress by targeting protein degradation with proteasome inhibitors (PI) has revolutionized the treatment of MM. However, resistance to PI is inevitable and represents an ongoing clinical challenge. Our first-in-human study of the selective inhibitor of RNA polymerase I transcription of ribosomal RNA genes, CX-5461 has demonstrated a potential signal for anti-tumor activity in three of six heavily pre-treated MM patients. Here we show that CX-5461 has potent antimyeloma activity in PI-resistant MM preclinical models in vitro and in vivo. In addition to inhibiting ribosome biogenesis, CX-5461 causes topoisomerase II trapping and replication-dependent DNA damage, leading to G2/M cell cycle arrest and apoptotic cell death. Surprisingly, the addition of PI does not enhance the therapeutic benefit of CX-5461. In contrast, CX-5461 shows synergistic interaction with the histone deacetylase inhibitor panobinostat in both the Vk*MYC and the 5T33-KaLwRij mouse models of MM by targeting ribosome biogenesis and protein synthesis through distinct mechanisms. Our findings thus provide strong evidence to facilitate the clinical development of targeting the ribosome to treat relapsed and refractory MM. Overall design: To investigate the molecular mechanisms underlying the synergistic response to CX-5461 and panobinostat, we performed RNAseq analysis in both p53-WT MM1.S and p53-null JJN-3 cells. MM.1S cells were treated with vehicle, 500 nM CX-5461, 50 nM panobinostat or the combination for 24 hr and cellular RNA was extracted for 3' RNA sequencing analysis (n=3).