Heterologous expression and activity assay of clade I and II N2O reductase genes using WP3 strain at 0.1, 20 and 40 MPa
The nos gene clusters includes Tat-dependent (clade I) and Sec-dependent (clade II) clades. Activity detection of microbial Sec-nosZ and Tat-nosZ enzymes from the hadal sediments under different hydrostatic pressures.