BEmins: Mineral surfaces as hotspots for microbes and element cycling in the Biodiversity Exploratories
Mineral surfaces as hotspots for microbes and element cycling in the Biodiversity Exploratories. Mesh bags (mesh size 50 micrometer) were placed in 5cm soil depth, meshes were filled with a mixture of sand and either geothit or illit mineral, harvest after remaining in soil for 5 years, each sample from mesh bag is acompanied by a soil sample taken direktly on top of the mesh bag. DNA was extracted from frozen material and fungal ITS2 region was amplified using PCR and sequenced using Illumina MiSeq.