Homeobox regulator Wilms tumour 1 (WT1) is displaced by androgen receptor at cis-regulatory sites in the endometrium of PCOS patients.
Polycystic ovary syndrome (PCOS) is a common gynaecological disorder, characterised by elevated circulating androgens and increased expression of androgen receptor (AR) and by downregulation of Wilms Tumour 1 in the endometrium, The objectives of this study are: 1) Understand the functional role of WT1 in regulating decidualization response in healthy endometrial tissue, and 2) understand how interactions between aberrant WT1 and AR expression in PCOS patients result in dysregulation of deciduallization processes Overall design: Endometrial stromal cells isolated from 3 healthy patients were pooled together, grown to confluency and treated with cAMP. One chromatin sample (containing genetic background from 3 different patients) was immunoprecipitated with an anti-WT antibody and subsequently submitted to ChIP-Seq