anlysis of FP chimeric in Nanopore sequencing
Nanopore sequencing enables the generation of long and ultra-long reads, which are crucial for applications such as large structural variation detection and genome assembly. However, false positive (FP) chimeric reads, particularly inverted repeats, can emerge during sequencing and impact somatic structural variation (SV) detection. Usually, artificial chimeric reads derived from amplification processes like 16S rDNA sequencing should be minimized during the PCR-free nanopore sequencing process. Surprisingly, through systematically evaluating the nanopore reads of microbiota and human DNA standards, we found the ligation-based library preparation and subsequent sequencing yielded potential artificial chimeric reads, mainly inverted repeats.