Gene expression profile at single cell level of sorted CD45+ cells from the GL261 tumor bearing hemisphere, treated with PBS / CSI / CSI+aPD1
We performed scRNA-seq to assess the effect of CpG-STAT3ASO+anti-PD1 therapy on immune cells from GL261 high grade glioma model. Overall design: The mice were implanted with GL261 cells. Subsequently, the mice received four intracranial treatments with either PBS, CpG-STAT3ASO, or CpG-STAT3ASO combined with anti-PD1, and were sacrificed on day 21. Tumor-bearing hemispheres were then isolated and dissociated. CD45+ cells were sorted and subjected to single-cell RNA sequencing (10X Genomics 3').