RNA-Seq of drug-treated and control 4T1 cells
Cell derived xenografts treated with DMSO, Oxa, APAP or OAP2 were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, mRNA was purified from total RNA using polyT and then fragmented with 10× RNA fragmentation buffer and the RNA-seq library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced. Overall design: Comparative gene expression profiling analysis of RNA-seq data for OAP2 treated cell derived xenograft and other drugs treated cell derived xenograft