RNA-seq of Saponaria officinalis tissues (leaves, stems and roots) in response to MOCK or 50 uM Methyl Jasmonate treatment at 6 and 24 hours
The experiment aims to identify genes encoding enzymes involved in the biosynthesis of triterpenoid saponins in Saponaria officinalis. RNA-seq data generated were generated from leaves, stems, and roots from 3 months old- hydroponically grown S. officinalis plants that were treated with 50 µM methyl jasmonate or a mock control. Samples were collected at 6 and 24 hours post-treatment. A total of 36 RNA samples, each consisting of triplicate biological replicates pooled from three plants, were collected. RNA was extracted using the ReliaPrep RNA miniprep system (Promega), sequenced on an Illumina HiSeq 6000 platform with 100 bp single-end reads, and mapped to the reference transcriptome using Salmon.