H3K27ac Hi-ChIP analysis in MOLT-4 and DND-41 cells.
H3K27ac Hi-ChIP analysis was performed in MOLT-4 and DND-41 cells to analyze active chromatin-chromatin interactions after DMSO or ACBI-1 treatment. Overall design: The cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP), tagmented with Tn5, subjected for library construction, and sequenced by the DNBseq platform.  Hi-ChIP was performed using an antibody against H3K27ac (Abcam: ab4729).