Gene expression profiles of null Pacman/Xrn1 mutant Drosophila testes
The null pacman allele pcm[14] causes at lethaility pupariation. Lethality can be rescued by driving expression of wild-type pacman in somatic cells during development using the UAS-GAL4 system. Use of the driver arm-GAL4 allows adult flies to develop, but pacman is not expressed in the germline cells. RNA was prepared from null pacman testes from rescued flies, parental controls (UAS line and GAL4 line) and from a genetic background control for pcm[14] (a line generated by a neutral excision of the P-element used to induce the pcm[14] deletion).   Total RNA was prepared from adult Drosophila testes. Sequencing of each genotype was replicated 3 times with a read depth of 12-15 million reads per replicate.