AreB, the nitrogen and carbon regulator in Aspergillus nidulans
Nitrogen and carbon repression general regulatory systems modulate the expression of target genes participating in utilization of alternative nitrogen/carbon sources, resulting in transcription only when preferred sources are limiting. In Aspergillus nidulans, the GATA transcription factor AreB was identified as nitrogen regulator whose activity depends on both nitrogen and carbon source (Conlon et al., 2001; Macios et al., 2012; Chudzicka - Ormanec et al., 2019). In this work, we performed a transcriptomic analysis of areB deletion and the wild type strains grown under different carbon/nitrogen conditions and showed that AreB          participates both in nitrogen and carbon regulation. Observed positive and negative effects of areB deletion are at least partially, indirect as the expression of some transcription factor coding genes is changed in areB deletion strain. Overall design: Comparative gene expression profiling analysis of RNA-seq data for areB? and the control areB+ (wild type) strains, grown under carbon repressing (1% glucose) or non-repressing (0.1% fructose); and under nitrogen repressing (10 mM ammonium tartrate) or non-repressing (5mM urea) conditions in three biological replicates.