Hepatocyte-macrophage crosstalk via the PGRN-EGFR axis modulates ADAR1-mediated immunity in the liver -Spatial Transcriptomics
ADAR1 is thought to be an immune suppressor maintaining self-tolerance to endogenous nucleic acids. Lack of ADAR1 results in MDA5 (Ifih1) activation and MDA5 deletion rescues ADAR1 null embryonic lethality. We generated liver specific ADAR1; MDA5 double KO mice and performed high throughput sequencing to investigate hepatic inflammation in these mice. Spatial transcriptomics (Visium, 10x Genomics) was performed on FFPE mice liver tissues. Constructed libraries were sequenced on the Novaseq 6000 platform. Over 250 million pairs of mappable reads for each sample were obtained. Overall design: Examination of spatially resolved transcriptomes of Ifih1-/- Adar1wt/wt Alb-Cre+, Ifih1-/- Adar1wt/flox Alb-Cre+, and Ifih1-/- Adar1flox/flox Alb-Cre+ mice liver tissues to investigate  the effects of Adar1 depletion against Ifih1 null background.