Genomic insights of Streptomyces melanosporofaciencs STM-2
We employed an integrated approach that involved physiological and biochemical characterization, genome mining, comparative genomics, and evolutionary analysis to elucidate the specific genomic determinants underlying the antifungal activity exhibited by STM-2, a novel Streptomyces melanosporofaciens strain, isolated from Chiayi County, Taiwan. Initial physiological and biochemical characterization, complemented by enzymatic screening, revealed the secretion of antifungal hydrolytic enzymes, including protease, CMCase, pectinase and xylanase, which actively play a role in fungal growth inhibition and fungal cell wall degradation. To elucidate the molecular basis of this activity, a whole-genome sequence was obtained and annotated, revealing a typical high-GC (71%) linear chromosome. The WGS analysis revealed large repertoires of genes that underlie the antifungal activity of Streptomyces melanosporofaciens. Our study has provided a complete genome of S. melanosporofaciens, which had not been done until now. The S. melanosporofaciens genome available at ncbi is a draft one.