Small RNA sequencing of the hypothalamic-pituitary-ovarian (HPO) axis in Bian chickens (PRJCA045239)
The egg-laying performance of chickens holds paramount importance in poultry production, with the Hypothalamic-Pituitary-Ovarian (HPO) axis serving as a critical regulatory mechanism in this physiological process. This study collected hypothalamus, pituitary, and ovarian tissues for small RNA-seq from Bian chickens in low- and high-laying groups. Differential expression analysis across hypothalamic, pituitary, and ovarian tissues revealed candidate miRNAs of laying performance between high- and low-yield groups. These findings advance our understanding of chicken reproductive regulation and offer actionable targets for breeding.