Gene expression profiling of MDA-MB-231 cells incubated with therapy induced senescence derived small extracellular vesicles
TNBC, associated with poor prognosis and high tumour recurrence, are often treated with anti-mitotic drugs. However, cells may bypass treatment-induced cell death via mitotic slippage, resulting in multinucleated polyploid cells and senescence activation. Senescent cancer cells represent a population of residual disease and are highly secretory. The SASP elicited is enriched in soluble cytokines linked to tumor recurrence and distant metastasis. In contrast, sEVs derived from senescent cancer cells represent an underappreciated aspect of SASP and its mechanistic role in mediating paracrine effects remains poorly-understood. Here, we show senescent sEVs as a distinct population of SASP that could elicit anti-tumor activity. Overall design: To investigate the effect of sEVs from therapy induced senescent breast cancer cells on recipient breast cancer cells, we have incubated MDA-MB-231 cells for 72h with No sEV, Cont sEV, TIS sEV isolated from donor MDA-MB-231 cells (DMSO or Nocodozole treated). We then performed gene expression profiling analysis using data obtained from RNA-seq of recipient MDA-MB-231 cells after 72 h sEV treatment. Comparative gene expression profiling analysis of RNA-seq data from MDA-MB-231 cells (No sEV, Cont sEV, TIS sEV)