Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [RNA-seq]
The gene-regulatory mechanisms behind the female germline piRNA pathway in ovaries of metazoan species remain largely unexplored. Here, using the Drosophila model and combinations of RNA-seq, ATAC-seq, and single-cell approaches, we reveal the transcription factors that regulate the female germline piRNA pathway in animal ovaries. Overall design: Differential RNA-seq analysis between the wild-type ovarian somatic cells (OSC) and the l(3)mbt knockout OSCs. Differential RNA-seq analysis between the OSCs and the fGS/OSS (mix of female germ-line stem cells/ovarian somatic sheet). Differential RNA-seq analysis between the germline and somatic cells FACS sorted from the transgenic vas-GFP fly ovaries. Differential RNA-seq analysis between the OSCs overexpressing the ectopic Ovo vector and the control OSCs nucleofected with the empty vector