Type III interferons induce pyroptosis in gut epithelial cells and delay tissue restitution  upon acute intestinal injury.
To determine the transcriptional programs initiated by IFN-? to delay tissue restitution, we isolated intestinal crypts from the small intestine of VilCREIfnlr1fl/fl mice or WT littermates that have  been irradiated and performed targeted transcriptomics analysis Overall design: WT mice and   VilCREIfnlr1fl/fl mice received 11 Gy ionizing radiation, with lead shielding of the upper body. Targeted transcriptomics was  performed  on  freshly  isolated  small  intestinal  crypts.Barcoded libraries were prepared using the Ion  AmpliSeq Transcriptome Mouse  Gene  Expression  Panel,  Chef-Ready  Kit  (A36412; Thermo  Scientific)