Single cell gene and protein expression of 2W:I-Ab specific cells from effector and memory T cells after aLm infection
Single-cell gene expression of CD4+ tetramer+ cells from WT B6 animals was determined by sRNAseq after aLm infection. Overall design: B6 mice were infected with aLm for 6, 21, or 60 days, treated wth an anti-ARTC2 nanobody before harvest, and sorted for CD4+ Tetramer+ cells. Post sort cells were hashtagged for each timepoint and feature barcoded for cell surface proteins and immune receptor mapping. Gene expression was determined by scRNAseq.