CHD3 and CHD4 coordinate gene expression programs to maintain ß-cell function and identity in vivo [ATAC-seq]
To establish the role of CHD3 and CHD4 on controlling gene expression programs in the beta cell, we generated a tamoxifen-inducible, beta-cell-specific knockout mouse models; control (MIP-CreERT; Chd3+/+; Chd4+/+; R26RtdTomato) and Chd3/4Db (MIP-CreERT; Chd3f/f; Chd4f/f; R26RtdTomato) Overall design: Dispersed islets from tamoxifen treated control and Chd3/4Db mice were flow-sorted, and ATAC-Seq performed.