Integrative Multi-omics Reveals RNA Editing Landscape and ADAR1-mediated Modulation of CDK13 Signaling in Esophageal Squamous Cell Carcinoma
Esophageal cancer ranks sixth in terms of high incidence and mortality rate worldwide and is particularly prevalent in Asia. The study aims to characterize RNA editome in esophageal squamous cell carcinoma and adjacent normal tissues, coupling with RNA immunoprecipitation of ADAR1 to identify ADAR1-dependent RNA A-to-I editing sties in esophageal cancer. CDK13, a cyclin-dependent kinase, undergoes editing that relies on ADAR1 enzymatic activity, was found exclusively associated with survival prognosis in primary ESCC but not in metastasis. We here provide ADAR1 RIP-seq of two ESCC cell lines (KYSE30 and KYSE150), transcriptome and phosphoproteome of CDK13-depleted and control scramble cell lines in KYSE30 unraveling potential substrates of CDK13 in ADAR1-CDK13 axis. These findings collectively provide insights into the pivotal role of RNA editing in primary tumors and the potential mechanisms underlying ADAR1-regulated CDK13 editing in ESCC progression.