YB-1 inhibits proliferation of mesothelioma cells through multiple mechanisms
Here we utilized an unbiased whole RNA-seq approach to characterize the transcriptional effects of YB-1 knockdown in three mesothelioma cell lines (MSTO, VMC23 and REN cells). Bioinformatic analysis showed that YB-1 knockdown regulated 150 common genes enriched for mitotic genes, integrins and extracellular matrix. However, each cell line also displayed a unique transcriptional signature, with differential deregulation of cell death, the cell cycle, and p53 signaling observed across the three cell lines. Overall design: Next-generation RNAseq analysis of YB-1 knockdown across triplicate biological repeats in 3 mesothelioma cell lines