16SrRNA Sequencing before and after animal experiments
The feces of 15 beagle dogs were collected before and after the experiment and sent to Novogene for 16SrRNA sequencing. The process is as follows: fecal bacterial genomic DNA is extracted and PCR amplified using universal primers (515F and 806R).The PCR products were purified using magnetic bead purification. Samples were mixed in equidensity ratios based on the concentration of PCR products. After thorough mixing, the PCR products were detected, and target bands were recovered. Sequencing libraries were generated, and indexes were added. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.