Gene expression profiling of ST-HSCs and MPP2 cells conditionally expressing MLL-ENL by RNA-seq
To investigate the molecular mechanism of leukemic transformation by MLL-ENL in HSCs and immature progenitors, we preliminarily performed comprehensive gene expression profiling of ST-HSCs and MPP2 cells by RNA-seq using a mouse model enabling to conditionally express MLL-ENL at an endogenous-like expression level. Overall design: ST-HSCs and MPP2 cells from our model mouse were treated with 4OHT conditionally inducing expression of MLL-ENL, or with ethanol as a vehicle control.Total RNA was extracted from 4 kinds of samples, respectively, and analyzed by RNA-seq. The induced cells were compared with the individual contorols.