Effects of dihydrotestosterone on glioblastoma cells U87
We analyzed the whole transcriptome of glioblastoma cell U87 samples cultured with different dihydrotestosterone levels. Differentially expressed mRNAs were selected for deep analysis. Especially the Differentially expressed genes that associated with tumor progression and androgen synthesis Overall design: The experiment was then divided into three groups: control group(3samples), charcoal-stripped fetal bovine serum group (CFBS,3samples), and high-level androgen group (Double Hydrogen Testosterone (DHT,3samples), 20µmol/L). U87 cells were cultured for 72h.