Genome-wide CRISPR screen with Cisplatin and Doxorubicin for Osteosarcoma
We bought the Toronto CRISPR human knockout library (TKOV3, Addgene #90294), and conducted genome-wide CRISPR screen on SaOS2 cells under the conditions of: control (CTRL_1, CTRL_2, CTRL_3), 0.25 uM Cis (CIS_1, CIS_2, CIS_3), 0.0025 uM Dox (DOX_1, DOX_2, DOX_3), and combination of Cis and Dox (CMB_1, CMB_2)