Transcriptomic profiling of Angiotensin II induced fibrotic myocardial tissue in response to EHP-101 or Losartan
In order to obtain a global perspective of the effect of EHP-101 and Losartan on cardiac fibrosis, we performed a mRNA-Seq analysis of the myocardial tissue changes in response to Ang II, Ang II + EHP-101 and Ang II + Losartan. Overall design: Eight-week old male C57/BL6 mice were housed under controlled conditions with free access to standard food and water. First, mice were anesthesized and exposed to a subcutaneous infusion of Ang II (or saline) using osmotic pumps during 28 days. In the last two weeks, mice were treated with oral EHP-101 (20 mg/kg), dilued Losartan in the drinking water (0,6 gr/L) or vehicle. Finally, mice were sacrified by cervical dislocation and skin, heart, aorta, lung and kidney were removed and processed for further analysis.