Sequencing data of AQP2-specific inhibitor Steviol against colorectal cancer
In order to explore the mechanism of Steviol, we used RNA-seq technology to extract and analyze the total RNA of cells treated with DMSO ,Steviol, MG132 and Steviol+MG132 and analyzed the RNA data. Overall design: After reaching 80% density, HCT116 cells were divided into four groups, with no less than 5×10^6 cells in each group. One group was treated with 80µM Steviol drug,One group was treated with 2µM MG132 drug One group was treated with 80µM Steviol drug +2µM MG132 drug and the other group was treated with DMSO as a control. Both groups of cells were incubated at 37°C. One day later, the original culture medium was removed and washed with 2 mL PBS using an RNaseA-free pipette tip. 1 mL of total RNA extraction reagent (Biyuntian, China) was added to the culture dish until the cells were completely covered. The culture dish was gently shaken and allowed to stand for 2-3 minutes. Mixed by blowing with an RNaseA-free pipette tip and aspirated into an RNaseA-free cryovial. Quickly frozen in liquid nitrogen and stored in a -80°C freezer. Subsequent RNA sequencing analysis was performed by Sangyo Bioengineering (Shanghai).