Description of a new B*35:01 allele identified in a a french  volunteer bone marrow donor
Here we describe a novel B*35*01 allele, identified in a a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*02:01 ,*02:01 B*35:01New, B*44:02 C*04:01 ,*04:01 DRB1*01:01 ,*08:01 DQA1*01:01 ,*04:01 DQB1*04:02 ,*05:01 DPA1*01:03 ,*02:01 DPB1*03:01 ,*09:01 HLA-B**35: 01New allele differs from B*35 :01 by one nucleotide substitution at position 38 of exon 7 (position 1083 of cDNA), where  base A is replaced by G  without chang of amini acide . In fact 337th ACAC becomes ACG, both of them code for Thr.