RNA-Seq of isolated primary human hepatocytes carrying the PNPLA3-I148M mutation compared to the WT
Although, there has been strong correlation between PNPLA3-I148M variants and metabolic dysfunction-associated steatotic liver disease (MASLD), the mechanism by which this variant drive the disease is not well understood. To this end, we perform this experiment to understand the transcriptomic changes that can be attributed to this variant. The total RNA was extracted from isolated primary human hepatocytes using RNeasy Plus Micro Kit (Qiagen). The sequencing library was constructed by following the Illumina Nextera XT Sample Preparation Guide. One nanogram of input cDNA was tagmented and amplified using the Illumina Nextera XT kit. Equimolar amounts of each sample were finally pooled and sequenced on an Illumina Nextseq 500 system, using a paired-end 75-bp strategy