Repeated gains in UV sensitivity in frogs traces the degeneration of jumping
Eye tissues were collected from four Asian toad (Bufo gargarizans) individuals (two males: SWU32600 SWU32601;and two females: SWU32603 SWU32604), and total RNA was extracted using the TIANGEN RNAprep Pure Tissue Kit (DP431, TIANGEN Biotech, Beijing). The RNA was then used for MGI sequencing. All animal procedures were approved by the Institutional Animal Care and Use Committee at Southwest University (LAC2023-2-0091). Raw sequencing data were filtered using Trimmomatic v0.39 44, retaining clean reads of at least 36 bp.