RNA binding properties of PDHA2, TEX30 in testis; RNA binding properties of HA-NONO and HA-NONO?ER in 3T3L1 cell line.
To investigate the RNA binding proterties of PDHA2, TEX30 in testis; RNA binding properties of HA-NONO and HA-NONO?ER in 3T3L1 cell line, we collected wildtype testis and HA-NONO/HA-NONO?ER overexpressed 3T3L1 cells. We then performed eCLIP-seq respectively and convey subsequent bioinformatic analysis. Overall design: RBP_eCLIP-seq; RNA substrate analysis of eCLIP-seq data for TEX30 and PDHA2 in testis; Comparative RNA binding specificity and affinity analysis of eCLIP-seq data for HA-NONO and HA-NONO?ER in 3T3L1 cell line