Capturing and tracking clonal T cell response to cancer neoantigens
C57BL/6-FoxP3EGFP mice were inoculated subcutaneously with B16F0 cells and treated with either anti-CTLA4 immune checkpoint inhibitor or PBS as control. CD4 Th and Treg cells were isolated from tumor draining and non-draining lymph nodes and analyzed with bulk RNA sequencing for dissection of antitumor immune pathways.