Comparative gene expression profiling analysis of hippocampus and liver RNA-seq data for young (3-month-old) and aged (22-month-old) WT mice in both control and ethanol feeding conditions.
Alcohol consumption is believed to affect Alzheimer's disease (AD) risk, but the contributing mechanisms are not well understood. A potential mediator of the proposed alcohol-AD connection is autophagy, a degradation pathway that maintains organelle and protein homeostasis. The purpose of this study is to explore whether chronic alcohol consumption worsens the age-related decline in lysosomal biogenesis in the brain and exacerbates cognitive decline associated with aging. To explore the effect of alcohol on brain autophagy, we exposed young (3-month old) and aged (23-month old) mice to chronic plus binge alcohol-feeding paradigms and the mouse brain and liver RNA were extracted followed by RNA sequencing analysis. Overall design: The mice were subjected to the Gao-Binge alcohol model. RNA sequensing analysis was performed using the hippocampus and liver tissues. Gene expression profiling analysis was conducted using data obtained from RNA-seq of 4 different groups.