Staphylococcus aureus USA300 LAC transcriptome analysis during infection of RAW264.7 macrophages
RAW264.7 macrophage cells were infected with Staphylococcus aureus USA300 LAC and the corresponding mutant strain. Intracellular bacteria were recovered after infection, and total RNA was extracted for transcriptome sequencing. RNA-seq was performed to analyze gene expression changes in S. aureus during intracellular survival in macrophages compared with the control condition.