Peptide binding to human IgE antibody
A phage-displayed peptide library was biopanned against human IgE. Phage clones were screened using the bead array method. The nucleotide sequences of positive phage clones were analyzed. The 36-nucleotide sequences were translated into 12-mer peptides. These 12-mer peptides were synthesized and tested against human isotype antibodies. The synthetic peptides specifically bound to human IgE but did not bind to human IgA, human IgG, human IgM, goat IgG, mouse IgG, or rabbit IgG.