An ESX-5 full locus deletion in Mycobacterium tuberculosis alters mycomembrane composition and boosts ESX-1 substrate secretion
Mycobacterium tuberculosis (Mtb) uses a specialized secretion system, ESX-5, to export numerous proteins during infection. Here, we found that deleting ESX-5 from Mtb not only prevented secretion of many ESX-5 substrates but also impacted other ESX systems. The M.tb esx5 mutant had increased ESX-1 substrate secretion but reduced ESX-3 expression.