RNA-seq Analysis of mRNA profiles in ß-Gal-overexpressing, and HuR-overexpressing primary hepatocytes.
Purpose: The goal of this study is to investigate how HuR regulates hepatocyte death. Methods: mRNA profiles of ß-Gal-overexpressing, and HuR-overexpressing primary hepatocytes were generated by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm38.p6) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3. Conclusion: Our study represents the detailed analysis of mRNA profiles in HuR-overexpressing primary hepatocytes, generated by RNA-seq technology. Our results show that 2677 genes were upregulated and 2712 genes were downregulated following HuR overexpression. GO analysis indicated that the upregulated genes were primarily related to inflammation response and apoptotic signaling pathway. These data indicate that upregulation of HuR in hepatocytes leads to cell death. Overall design: mRNA profiles in ß-Gal-overexpressing, and HuR-overexpressing primary hepatocytes were generated by deep sequencing using Illumina Novaseq 6000 platform (n=3 for each group).