PIKfyve controls dendritic cell function and tumor immunity II
To understand the mechanism of Pikfyve inhibition in dendritic cells, we cultured conventional dendritic cells isolated from the bone marrow of wild-type mice and performed transcriptome profiling on RNA extracted from cells treated with DMSO or apilimod for 3 or 8 hours on Day 6 in culture. Taken together, these data demonstrate the PIKfyve mediates suppression of DC transcriptional maturation programs through the alternate/non-canonical NF-?B regulatory pathway. Overall design: Comparative gene expression profiling of RNA-seq data from primary cultured conventional dendritic cells isolated from wild-type mice treated with DMSO or the PIKfyve inhibitor, apilimod.