RNA sequencing comparison of B7-H3.TMIGD2 and B7-H3.CD28.4-1BB CAR-T cells in vitro
CAR-T cell costimualtory domains can alter CAR-T cell phenotypes. We therefore performed RNA sequencing to understand the effect of TMIGD2 vs CD28.4-1BB comstimulation in B7-H3 CAR-T cells after in vitro coculture. Overall design: We cocultured CAR-T cells and HCC827 tumor cells for 72 hours. After isolation of T cells and T cell RNA, we performed RNA sequencing