VIGS experiment of GhMAPK10
To investigate the role of GhMAPK10 in drought stress adaptation, we conducted the virus-induced gene silencing (VIGS) construct targeting GhMAPK10 (TRV156:GhMAPK10). Both WT and VIGS seedlings were subjected to an 18% polyethylene glycol (PEG-6000) treatment for 4 hours to simulate acute drought stress.RNA sequencing was subsequently performed on all four groups,TRV156_0h,TRV156_D4h,GhMAPK10_0h,GhMAPK10_D4h.