Exploring Enhancers and Promoters in Xenopus tropicalis Osteoblasts through Chip-Seq analysis
Primary osteoblast from NF60 Xenopus tropicalis larval skulls were extracted by a series of collagenase/trypsin treatments, and directly (i.e. without culture) subjected to ChIP-Seq using antibodies against total H3 as well against as the H3K4me3, H3K4me1, H3K27ac and A3K27me3 histone marks.