RNA-seq analysis of cytosolic mitochondrial RNA in DRP1-deficient HeLa cells
This project aims to characterize mitochondrial RNA (mtRNA) species present in the cytosolic fraction of HeLa cells under conditions of altered mitochondrial dynamics. RNA sequencing was performed on cytosolic RNA isolated from wild-type and DRP1-knockout HeLa cells treated with control siRNA or siRNA targeting MFN1/2. These experiments were designed to comprehensively analyze mtRNA released into the cytosol. We observed that loss of DRP1 leads to the accumulation of mitochondrial transcripts in the cytosolic fraction, which is associated with activation of innate immune responses. The dataset provides a resource for investigating the relationship between mitochondrial dynamics, cytosolic mtRNA release, and RNA-mediated immune signaling pathways.