Full-Length 16S Sequencing of a Defined Microbial Community in 6 dpf Zebrafish Larvae
This project provides near full-length 16S rRNA gene sequencing of a defined ten-member microbial community (Mix10) colonizing the skin and gastrointestinal tract of 6 days post-fertilization (6 dpf) gnotobiotic zebrafish larvae. Germ-free zebrafish were colonized under controlled conditions with a consortium of bacterial strains previously isolated from zebrafish-associated environments.Near full-length 16S rRNA gene amplicons spanning the V1- V9 regions were generated using universal primers 27F and 1492R. Single Molecule Real-Time (SMRT) sequencing was performed on the PacBio Revio platform to obtain high-fidelity long-read sequences, enabling species-level resolution of community composition.These data serve as a compositional reference dataset to support and benchmark strain-resolved spatial imaging analyses performed using CIPHR-FISH (Cross-Hybridization Inference for Phylogenetic Resolution Fluorescence In Situ Hybridization). Integration of sequencing and imaging enables coordinated assessment of microbial composition and spatial organization within a controlled vertebrate host system during early development.