Single-cell transcriptomics reveals diverse and complex gene expression alterations in human fetal spinal cord [Visium]
We analyzed the transcriptomics of single cell of human fetal spinal cord at GW8-Gw12  using 10x Genomics. Overall design: The Visium Spatial Tissue Optimization workflow includes placing tissue sections on 7 Capture Areas on a Visium Tissue Optimization slide. The sections are fixed, stained, and then permeabilized for different times. mRNA released during permeabilization binds to oligonucleotides on the Capture Areas. Fluorescent cDNA is synthesized on the slide and imaged. The permeabilization time that results in maximum fluorescence signal with the lowest signal diffusion is optimal. If the signal is the same at two time points, the longer permeabilization time is considered optimal.