This study presents the coding sequences (CDS) of Gonadotropin-Releasing Hormone 2 (GnRH2) and Gonadotropin-Releasing Hormone 3 (GnRH3) genes from several Indonesian freshwater teleost species, including Osphronemus goramy, Anabas testudineus, Hemibagrus nemurus, Mystus sp., Barbonymus balleroides, Tor tambroides, and Barbonymus gonionotus. Each sequence represents a single coding region, annotated with gene product information and translational capability.
Gonadotropin-Releasing Hormones (GnRHs) are key neuropeptides regulating reproductive physiology in teleost fish. In this study, we sequenced and annotated the coding sequences (CDS) of GnRH2 and GnRH3 genes from multiple Indonesian freshwater teleost species. A total of nine sequences were obtained, each corresponding to a single CDS with partial 5' and 3' ends. The sequences were collected from specimens in Indonesia and verified for translational potential. GnRH2 sequences from six species ranged from 84 nucleotides, while GnRH3 sequences from three species were 93 nucleotides long. This dataset provides insight into sequence variation across species, contributing to understanding the evolutionary conservation of reproductive hormones in tropical freshwater fishes.