Monosome and disome profiling of Flag-STAU1 Flp-In 293 T-REx cells
Monosome and disome profiling was performed on Flag-STAU1 Flp-In 293 T-REx to study the causes of ribosomal collisions, and whether this may be modulated by the presence/absence of Staufen-1.   Cells were treated with either an siRNA targeting STAU1 transcript (4x samples) or a control siRNA (2x samples). Two of the four samples treated with the STAU1 siRNA had siRNA-resistant STAU1 mRNA expression induced by doxycycline (rescue). Sequencing libraries from monosome and disome fractions were generated in parallel from the same samples.  Note that unique molecular identifiers/random barcodes (UMIs/RBCs) were included in the sequencing experiment. Each UMI has been moved to the fastq read name of each read. For example \"xxxxxxrbc:AGCCAAT\" in the read name signifies that the given read had a UMI of \"AGCCAAT\". Using these UMIs, PCR duplicates can be removed with UMI-Tools following read alignment.