The effects of NAT10 on heart development
The goal of this study is to investigate how NAT10 regulates heart development Overall design: mRNA profiles in hearts of Nat10flox/flox and cardiomyocyte-specific Nat10 knockout (Nat10-CKO) mice were generated by deep sequencing using Illumina Novaseq 6000 platform (n=3 for each group). Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from hearts of Nat10flox/flox and Nat10-CKO mice at 15 days old. Three independent biological replicates for each group were used for RNA-seq. RNA-seq was performed by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm38/mm10) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3. Our study represents the first detailed analysis of heart transcriptomes from Nat10flox/flox and Nat10-CKO mice, generated by RNA-seq technology.