Chromatin profiling following SWI/SNF inhibition in H295R or HEK 293T cells [ChIP-seq]
To assess the genome-wide effect of acute SWI/SNF inhibition on SF-1/ß-catenin chromatin binding, we treated H295R or HEK 293T cells with 1 µM of SWI/SNF inhibitor BRM014 or DMSO vehicle control for 1 hour and performed ChIP-seq. Pronounced loss of chromatin occupancy was observed for SF-1 and ß-catenin at enhancer sites bound by ARID1A-containing cBAF complexes. Overall design: Chromatin occupancy profiling in H295R or HEK 293T cells treated with SWI/SNF inhibitor or DMSO vehicle control.