Effect of Humanin treatment on brain tumor vascular cells
RNA sequencing analysis was performed on at least 1000 tumor-derived bulk-sorted mouse cells. Quality control of total RNA samples after RNA isolation with the Agilent Bioanalyzer revealed acceptable RNA quality for provided samples. Libraries were successfully prepared from these RNA samples using the Illumina TruSeq® Stranded mRNA technology. This approach uses fragmentation and sequencing adapter ligation in combination with a poly-T oligo pulldown. RNA sequencing was performed on the Illumina NextSeq500 next generation sequencing system (Illumina) and its high output mode with two runs of 1x150 bp single-end read chemistry and a high amount of high quality reads was generated.