RNAseq profiling of Col-0 and lox4 mutant inoculated by fungus Alternaria alternata in Arabidopsis
To uncover the specific role of lox4 in EX1-dependent 1O2, researchers employed RNA-seq technology to analyze the expression patterns of 1O2-responsive genes in Col-0 and lox4 mutant plants following inoculation with Alternaria alternata necrotic strain. Methods RNA-seq libraries were sequenced on the Illumina HiSeq X Ten platform following standard Illumina protocols. Overall Design: At least three 3-week-old Col-0 or lox4 mutant plants were inoculated with 3mm-PDA plugs containing Alternaria alternata . Two rosette leaves per plant were harvested at 0, 6, and 12 hours post-inoculation and immediately stored in liquid nitrogen. Three independent replicates were performed.