Transcriptome analysis of the adipose tissue in emu
The emu (Dromaius novaehollandiae) is a large ratite and has been predicted to become a useful poultry producing high quality oil. We conducted a transcriptome analysis to identify genes that exhibit high expression in subcutaneous fat, with the aim of developing genetic markers linked to their production traits. Fatty tissue was obtained from a 2-year-old emu, sourced from Okhotsk Emu Farm in Hokkaido, Japan, which had been slaughtered for the purpose of the industrial use. Total RNA was extracted by QIAzol reagent (Qiagen, Hilden, Germany) according to the manufacturer's protocol. The cDNA library was constructed by using TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). DNA sequencing was conducted by using HiSeq2500 (Paired-end 2Ã?100-bp) and the FASTQ file was formatted by bcl2fastq-v2.20.0.422.