Comparison of two materials effection on mice bone marrow mononuclear cells and cardiac repairing
Biomaterials can improve cardiac repair combined with transplantation of bone marrow mononuclear cells (BMMNCs). In this study, we compare the phenotype and cardiac repair between human heart valve-derived scaffold (hHVS) and nature protein/polycaprolactone (NP/PCL) anchored BMNNCs. Methods and Results: BMMNCs were obtained from mice five days following myocardial infarction. Subsequently, BMMNCs were separately cultured on hHVS and PCL. Proliferation and cardiomyogenic differentiation were detected in vitro. Cardiac function was measured after transplantation of cell-seeded cardiac patch on MI mice. After that, the BMMNCs were collected for mRNA sequencing after culturing on the scaffolds. Upon anchoring onto hHVS or PCL, BMMNCs exhibited an increased capacity for proliferation in vitro, however, the cells on hHVS exhibited superior ability cardiomyogenic differentiation ability. Moreover, both BMMNCs-seeded biomaterials effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. Cell-seeded hHVS was superior to cell-seeded PCL.  Conclusion: BMMNCs on hHVS show better capacity in both cell cardiac repairing and improvement for cardiac function than on PCL. Compared with seeded onto PCL, BMMNCs on hHVS have 253 genes up regulated and 189 genes down regulated. The reason of hHVS is better than PCL for a scaffold for BMMNCs might be the optimized method of decellularization let more cytokines in ECM retained. Overall design: BMMNCs were separately cultured on hHVS and PCL. Proliferation and cardiomyogenic differentiation were detected in vitro.the BMMNCs were collected for mRNA sequencing after culturing on the scaffolds.