Single-cell transcriptomic analysis of cancer-associated fibroblasts under hypoxic and normoxic conditions
This study aims to investigate the differences in gene expression between cancer-associated fibroblasts (CAFs) cultured under hypoxic (1% O2) and normoxic (21% O2) conditions at single-cell resolution. Using single-cell RNA sequencing, we characterized the transcriptional heterogeneity and identified hypoxia-responsive gene signatures in CAFs. This analysis provides insights into how hypoxia modulates CAF phenotypes and functions in the tumor microenvironment, potentially revealing novel therapeutic targets for cancer treatment.