Foxc1 ChIP-seq in Gli2-mGFP NIH3T3 cells stably expressing Foxc1 ORF
To interrogate occupany sites of Foxc1 transcription factor whole genome ChIP-sequencing was performed on Gli2-mGFP NIH3T3 reporter cells stably expressing Foxc1, using different anti-Foxc1 antibodies. Overall design: Two independent Foxc1 antibodies (ab1, ab2) were used, together with an isotype-matched control immunoglobulin as control for Foxc1 peak specificity.