Cardiac myocyte RNA-seq, Myc overexpression
To determine the effect of Myc expression on gene regulation and injury response in adult cardiac myocytes, cardiac-specific expression of Myc family genes (Myc, Mycl, Mycn) and GFP was induced using an AAV vector in 6-week-old C57BL/6J male mice. Two weeks later, cardiac myocytes were isolated, and RNA-seq was performed (PolyA enrichment, PE150).