Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (ChIP-Seq)
Here we studied the budding yeast Lachancea kluyveri, a cousin of the model Saccharomyces cerevisiae, in order to try to understand the mechanism responsible for the absence of meiotic recombination on its almost entire sex chromosome (Brion et al. 2017). We performed protein-DNA interaction mapping of Rec8, Red1, Hop1 and Zip3 proteins by ChIP-seq. Briefly, we observed an enrichment of Zip3, a protein known as a marker of type I crossing-overs, where peaks of DSBs were detected by CC-seq and a virtual absence of Zip3 within Lakl0C-left. For Rec8, a meiotic specific protein part of the cohesin complex, we detected enriched peaks at centromeres and inside intergenic regions flanked by convergent ORFs everywhere in the genome Lakl0C-left included. Remarkably, Red1 and Hop1, known to form the lateral element of the synaptonemal complex and important for DSB formation, were enriched mainly at Rec8 peaks except at centromeres and particurlarly on Lakl0C-left where they are completely absent. Overall design: This data set contains maps of Rec8, Red1, Hop1, Zip3 (FLAG tagged at their carboxy-termini) and untagged DNA contacts in the L. kluyveri genome generated by ChIP-seq of meiotic CBS 10367 cells. Cells were treated 1, 4 and 6 hours after sporulation induction.