The impact of  chemical modulator of cytoskeleton in murine CD8+ T cell.
T cells cytoskeleton is invovlved in the recognition of the antigen-presenting cells and target cells. So we used  chemical modulators of cytoskeleton (Blebbistatin) to treat murine CD8+ T cells. Then RNA-seq was performed. We found that Ble, compared with the control group, made  significantly changes in the mRNA levels of murine CD8+ T cells. Overall design: Total RNA was isolated from murine T cells treated with Veh., Ble (2 µM) for 24 hr by using TRIzol-chloroform method. Then RNA-seq was performed by using those RNA.