Functional analysis of plant translation regulation (on the tomato model) under cold stress
In the presented study, we analyzed the physiological, biochemical and molecular responses of experimental plants under low temperature conditions. The aim of the study was to investigate the differences in expression profiles in the total transcriptome and in its translated fraction. To more fully cover possible strategies for plant acclimation to cold, we selected the following experimental conditions: control (intact plants), stress, hardening, and a combination of hardening and stress. Since the goal of this work was to study the differences in expression profiles at both the transcription and translation stages, we used separate approaches to obtain these experimental data: for transcription -- sequencing of the total transcriptome, for translation -- sequencing of transcriptome fractions obtained during polysome profiling and corresponding to the translated mRNAs: monosomes and polysomes. Thus, for 4 experimental conditions and 3 types of mRNA libraries, 12 samples were obtained. Sequencing was performed in duplicate. The obtained data will allow us to evaluate the contribution of transcription and translation separately to the regulation of cold acclimation in tomato plants and, probably, to identify the molecular mechanisms responsible for this. Overall design: The experimental design included 4 types of temperature conditions: - control (intact plants) : Control - stress : Stress - hardening : Hardening - combination of hardening and stress : Combo and 3 different mRNA fractions: - total transcriptome : total - polysomes : poly - monosomes : mono Total: 12 different samples