Compartmentalisation proteomics revealed endolysosomal protein network changes in goat model of atrial fibrillation
To identify the role of the endolysosomal (EL) protein network in atrial fibrillation (AF), we applied a newly developed organelle isolation method to the AF goat model. To accomplish the goal, we used proteomics, transcriptomics, integrated analysis, enzyme assays, electron tomography, and western blotting and identified several regulating EL proteins, related protein networks and pathways. Overall design: In order to understand the endolysosomal protein level changes in AF goat model we conducted transcriptomics,proteomics, and integrated analysis. Furthermore lysosomal enzymatic activities and structural changes were examined using techniques such as, enzyme assays, western blotting and electron tomography. AF was induced and maintained in female goats (C. hircus) for 6 months and left atrial tissue were obtained by an open chest sacrifice experiment in (N=4 AF and N=4 sham controls without induced AF).