Tomography RNA sequencing of  consecutive cryosections of thoracic aortic dissection tissues
We used Tomoseq technology,a method for high sensitivity spatiallyresolved transcriptomics, to obtain a genome-wide gene expression signature with high spatial resolution spanning from the rupture area to the remote area on thoracic aortic dissection sample.A single-slice with a thickness of 5µm and a continuous collection of 20 slices for RNA extraction is the best slice parameter. Serial frozen sections with a total thickness of 100um were placed in a centrifuge tubeThe single tissue was cryosectioned into 20 continuous slices of 5 µm thickness into an eppendorf tube, 30 tubes in total,numbered as AD1-AD30.AD1-AD30 are the tube numbers, representing the rupture area to the remote area.RNA extractionwas performed for continuous section in each tube.Among them, AD5, AD11, AD16, AD28 and AD30 had low RNA concentrations or RIN/RQN 219 ratios; therefore, they were excluded from the analysis for RNA amplification, library construction and RNA sequencing.