Amplicon Sequencing Analysis of the Bacterial Microbiome of Lagotis brevituba
This project aims to analyze the diversity of endophytic microorganisms in Lagotis brevituba from different regions of the Qinghai-Xizang Plateau and their association with secondary metabolites. In this study, 12 samples of rhizosphere soil, root systems, and plant specimens of Lagotis brevituba were collected from multiple locations on the Qinghai-Tibet Plateau, and the physicochemical properties of the soil were systematically determined. Using high-throughput 16S and ITS rRNA sequencing technologies, we analyzed the diversity of bacterial and fungal communities, as well as the diversity and composition of these microbial communities. Concurrently, high-performance liquid chromatography (HPLC) was employed to quantitatively determine the concentrations of 10 key secondary metabolites. Furthermore, correlation network analysis and redundancy analysis were utilized to investigate the relationships among soil physicochemical factors, microbial communities, and secondary metabolites. The quality of extracted genomic DNA was assessed via 1% agarose gel electrophoresis, whilst DNA purity and concentration were quantified spectrophotometrically using the NanoDrop2000 (Thermo Scientific, USA). The V5-V7 region of the bacterium was amplified using primer pairs 799F-1392R and 799F-1193R. The ITS1 region of the fungi was amplified using the primers ITS1F-ITS2R. The data will provide a theoretical foundation for establishing an environment-microbiome-metabolite association for the medicinal plant Lagotis brevituba on the Qinghai-Xizang Plateau.