RNA-seq of 4T1 tumor samples after recombinant oncolytic adenovirus and anti-PD-1 combination therapy (PRJCA019936)
To systematically identify the changes in the gene expression of key molecules after combination therapy in 4T1 tumors. Flash-frozen tumors (n=3 mice per treatment group) were pulverized, and total RNA was extracted using the RNeasy Mini Qiacube extraction kit according to the manufacturer's protocol. Poly(A)-tailed mRNA was enriched, and an RNA-seq library was constructed by the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer's instructions. RNA-seq data were generated on an Illumina Nextseq 550 using the 75-bp pair-ended running mode. After removing reads with sequencing adaptors, unknown reads with N and low-quality reads, clean reads were mapped to a reference genome.