Raw sequence reads (16S rDNA, V4 regions) of activated carbon from drinking water biological filter and enrichment samples (glass beads)
Biological activated carbon (BAC) was collected from an up-flow biological filter at a drinking water treatment plant (DWTP) in Japan. DNA was extracted from the BAC for amplicon sequencing of the 16S rDNA V4 region. Bacteria were detached from the BAC using PBS, and glass beads were inoculated with the filtered PBS to form biofilms on the beads. After seven days of incubation, the glass beads were used in a column assay system for enrichment over two months. Three groups of influent substrates were: (1) autoclaved tap water (Tap0), (2) mineral salt medium with 300 ng/L 2-methylisoborneol (MIB) (MSM300), and (3) autoclaved tap water with 1000 ng/L MIB (Tap1000), respectively. After enrichment, DNA was extracted from the glass beads for 16S rDNA V4 region amplicon sequencing. Comparing microbial communities between the original BAC and enriched groups allows us to identify changes before and after enrichment and evaluate which microbes are enriched by MIB addition.