Gossypium hirsutum cultivar:TM-1 Raw sequence reads
Soil salinity is one of the most serious abiotic stresses limiting cotton (Gossypium hirsutum) growth and yield worldwide. Cotton is moderately salt tolerant, yet prolonged exposure to high salinity causes ion imbalance, oxidative stress, and growth inhibition. To better understand the molecular mechanisms of salt stress tolerance in cotton, we generated comprehensive next-generation sequencing datasets from cotton leaves.First, RNA sequencing (RNA-seq) was performed on cotton leaves under normal conditions, control treatments, and salt stress conditions, each with biological replicates, to characterize genome-wide transcriptional responses. These data provide insights into stress-responsive gene expression and the regulation of redox homeostasis.Second, DNA affinity purification sequencing (DAP-seq) was conducted for GhVOZ1 together with input control samples. These data reveal direct target genes and genome-wide DNA binding profiles of VOZ transcription factors that are implicated in salt stress regulation.Third, CUT&amp;Tag sequencing was performed using antibodies against RCC1 and VOZ1, along with control samples. These datasets identify chromatin-associated binding events and provide evidence for the recruitment of epigenetic regulators, such as SUVH4, to stress-related loci.Collectively, this BioProject provides raw sequencing data including RNA-seq, DAP-seq, and CUT&amp;Tag-seq from cotton leaves. The data support mechanistic studies of transcriptional regulation, epigenetic modification, and antioxidant homeostasis in cotton under salinity stress. These datasets will serve as a valuable resource for the plant biology community and facilitate the development of molecular strategies for breeding salt-tolerant cotton varieties.