RNA-seq data analysis of TgAP2XII-1 on Toxoplasma gondii merozoites development [ME49 XII-1 RNAseq]
To investigate transcriptional role of TgAP2XII-1 on type II Toxoplasma gondii (ME49 Tir1), an auxin inducible degron system was introduced to control the protein expression of TgAP2XII-1 and the target protein could be totally degradated in 3h after adding IAA. Overall design: Transgenic parasite ME49 iKD AP2XII-1-mAID-3HA was treated with IAA for 0h or 60h, then total RNA were extracted for deep sequencing.