Single-cell RNA-seq of H9 nEnd cells subjected to mesoderm differentiation.
The origin of human extraembryonic mesoderm (ExM) has been heavily debated. In order to address if ExM can be derived from primitive endoderm (PrE), we treated the human PrE cell line (also called na?ve extraembryonic endoderm (nEnd)) with a mesoderm induction protocol and sequenced the entire day 15 cell population.