eCV: Enhanced coefficient of variation and IDR extensions for reproducibility assessment of NGS experiments with multiple replicates.
Reproducibility assessment is essential in extracting reliable scientific insights from high-throughput experiments. Inconsistency between technical replicates poses a challenge, particularly evident in NGS technologies based on immunoprecipitations, where the need for reproducibility in peak identification is a well-acknowledged limitation. While the Irreproducibility Discovery Rate (IDR) method has been instrumental in assessing reproducibility, its standard implementation is constrained to handling only two replicates. In the current era of steadly growing sample sizes, facilitated by multiplexing and reduced sequencing costs, highly performing methods that handle any number of replicates are desirable. Overall design: RBP-eCLIP  experiment targeting RBFOX2 in samples from HEK293T cells. Information submitted for the following samples: RBFOX2_eCLIP_IP_1, RBFOX2_eCLIP_IP_2, RBFOX2_eCLIP_IP_3, RBFOX2_eCLIP_IP_4, RBFOX2_eCLIP_Input_1, RBFOX2_eCLIP_Input_2, RBFOX2_eCLIP_Input_3 and RBFOX2_eCLIP_Input_4.