Big Breakfast Study, a human study investigating the effects of breakfast meals containing different macronutrient profiles when consumed as part of a weight loss diet.
A human study to investigate the effect of two different weight loss diets with different macronutrient composition was carried out; a high protein breakfast meal, was compared with a higher fibre breakfast meal. The study design was a randomized, within-subject cross-over design with both diets fully controlled, and running for four weeks in length. There was a one week washout period between diets, during which time a maintenance diet was followed. Various biomarkers were measured, including the faecal microbiota composition. This was assessed using amplicon libraries of the V1-V2 hypervariable region of the 16S rRNA gene, sequenced using the Illumina MiSeq system. The study was carried out at the Rowett Institute at the University of Aberdeen under study code 804.