scRNA-seq and CITE-seq of T-BET/EOMES CRISPR deleted human NK cells
Peripheral blood NK cells that were CRISPR-edited to knock-out expression of T-BET or EOMES were prepared for scRNA-sequencing(10X Genomics).   The following samples were analyzed to elucidate the role of T-BET and EOMES in regulating mature human NK cell transcriptional profiles. Overall design: 12 samples scRNA-seq(Donors 1-3 in vitro and in vivo): TRAC and double TBX21+EOMES CRISPR edited NK cell generated from the same normal donors. 2 samples (Donors 89528 and 3227 in vitro only) CITEseq: TRAC, TBX21, EOMES, and double TBX21+EOMES CRISPR edited NK cell generated from the same normal donors are hashtagged into the same files