Gut microbiota of 123 Japanese adults
Feces samples from 123 Japanese adults were collected (Hisamatsu D. et al.). The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R), and multiplexed amplicon pyrosequencing was carried out using Illumina MiSeq.