Comparative transcriptomics (mRNAseq) of fruit fly heads exhibiting chronic proteostasis dysregulation in neuronal circuits.
This mRNA sequencing project aims to elucidate the transcriptomic repercussions of dysregulated neuronal proteostasis and the accumulation of transcription factors, including Xrp1 and crc, in condensates documented in multiple fruit fly mutant lines. Experimental design: Comparative gene expression from control (w1118) and two mutant (aln2 and norpA) adult Drosophila melanogaster heads. Mutant lines used were homozygous for the aln2 and norpA alleles and showed proteostasis dysregulation in neurons. All flies were reared unstressed on standard food, at 25C, and a 12:12 light-dark cycle then aged 3-5 days post eclosion. Three biological replicate single-end, 100bp libraries were prepared for each genotype using a TruSeq Stranded mRNA Library Prep Kit and sequenced on an Illumina NextSeq 2000 utilizing a P2 flowcelll.