Phase separation of YAP-MAML2 differentially regulates the transcriptome
SPARK-OFF tag does not perturb the core transcriptional function of YAP-MAML2.~2260 DEGs with = 1.5-fold change in transcript level, including ~1270 up-regulated genes and ~990 down-regulated genes can be identified from YAP-MAML2 overexpression HEK293T cells.We identified 88 DEGs (p-value < 0.01, |Log2FC| = 0.58, FDR < 0.1) that are regulated by YAP-MAML2 phase separation with = 1.5-fold change in transcript levels, including 44 up-regulated genes and 44 down-regulated genes Overall design: analyze the transcriptome difference in HEK293T cells which overexpress condensed YAP-MAML2 or diffusive YAP-MAML2. To control YAP-MAML2 phase separation, a rapamycin induced tool was applied to the cells. The control group is the effect on rapamycin alone. HEK293T express eGFP alone