Quantification of Bir1-dependent differential gene expression in swollen Aspergillus fumigatus conidia
This study aimed to understand the differences in gene expression when comparing Aspergillus fumigatus swollen conidia that overexpress an artifical Bir1 construct (strain published in Shlezinger et. al 2017) versus a parental control strain in RPMI + 0.5ug/mL voriconazole. The purpose of this experiment was to understand what basal expression differences may drive a decreased susceptibility to hydrogen peroxide upon subsequent treatment (not tested in this submission). The main results show that there is a significant upregulation of mitochondrial-related genes, included aconitate hydratase. These differences may account for increased susrvival upon cellular stressors in this strain background. Overall design: RNA-seq profiling of swollen Conidia representing various genotypes: A. fumigatus wt strain, ATCC46645 +Histone H2A:: mRFP fusion, ATCC46645 +Histone H2A:: mRFP fusion + Bir1OX, ATCC46645 +Histone H2A:: mRFP fusion + Bir1OX-N 1-446aa.