An arrhythmogenic cardiomyopathy pig model by PKP2 heterozygous knockout
Patients with arrhythmogenic cardiomyopathy (ACM) have a high incidence of ventricular arrhythmias and a significant risk of sudden death. However, the difficulty in obtaining myocardial tissue samples from ACM patients at the early stage of the disease, coupled with the lack of animal models that can simulate the characteristics of human ACM, has severely hindered in-depth research into its pathogenesis. Overall design: Total RNA was extracted from right ventricular tissue of PKP+/- and WT pig using RNeasy Mini Kit.). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated. The index of the reference genome was built using Hisat2 v2.0.5. and paired-end clean reads were aligned to the reference genome using Hisat2 v2.0.5.