Landscape of genome-wide DNA methylation profile of colorectal cancer metastasis
Genome-wide DNA methylation of colorectal cancer patients with lymph node metastases showed global loss of DNA methylation in CG-poor, non-CpG island (CGI) regions. Overall CGI methylation was increased in tumour samples. Differential methylation analysis of CGIs identified 60 putative biomarkers, with >20% increase in DNA methylation in both primary tumour and metastasis samples compared to normal adjacent tissue. Overall design: Enhanced methyl-seq of matched normal adjacent tissue, primary tumour and lymph node metastasis from 5 colorectal cancer patients was analysed.