The MMP-2 histone H3 N-terminal tail protease is selectively targeted to the transcription start sites of active genes [ChIP-seq]
Our study examines the role of MMP-2 mediated Histone H3NT proteolysis in U2OS cells. We find binding of MMP-2 at all active protein coding transcription start sites, and examine the role it plays in local chromatin regulation and changes in gene expression. Overall design: native ChIP-seq of 3XHA tagged Pro-MMP2 as well as a negative control (HA ChIP in WT cells not expressing tagged Pro-MMP2) and positive control (H3K4me3)