Transcriptomic sequencing of apple leaves
To explore the defense responses of different apple cultivars against C. fructicola, field-grown Fuji and Golden delicious were analyzed using RNA seqing. Fully expanded leaves of both cultivars were used for inoculation. The inoculated leaves were collected at 36 h after inoculation for RNA extraction and RNA-seq. Transcriptomic analysis revealed substantial differences in gene expression between the two apple cultivars after being inoculated with CF, including those involved in plant pathogen interactions, secondary metabolism, MAPK signaling pathway, plant hormone signaling pathway and circadian rhythm.