soil metagenome
This study investigates the impact of two exogenous organic acid compounds on the rhizosphere microbiome of Astragalus sinicus. Four treatments were established: (1) CK, non-spiked soil planted with A. sinicus; (2) N, 4-nonyl phenol (4-NP)-spiked soil planted with A. sinicus; (3) AN, 4-NP-spiked soil planted with A. sinicus and supplemented with aminosalicylic acid at 50 umol/L; (4) DN, 4-NP-spiked soil planted with A. sinicus and supplemented with 4,6-dioxoheptanoic acid at 50 umol/L.Rhizosphere soil samples were collected after 30 and 45 days of incubation. Metagenomic sequencing was performed on the Illumina NovaSeq 6000 platform (PE150) to characterize microbial community composition and functional gene profiles. This study aims to elucidate the mechanisms by which specific root exudate-like compounds shape rhizosphere microbial communities and their metabolic functions.