ACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload [ATACseq]
ATAC-seq was performed in NRCMs under four conditions: PBS, isobutyrate (5 mM, 36 h), siNc, and siAcad8 (48 h). ATAC-seq library was prepared using hyperactive Tn5 transposase to tagment accessible chromatin regions. Sequencing was performed on Illumina NovaSeq X Plus. Reads were aligned to mRatBN7.2 using Bowtie2. This dataset reveals that isobutyrate and Acad8 knockdown increase chromatin accessibility at hypertrophy-related gene promoters (e.g., Nppa, Nppb), supporting a role for histone isobutyrylation in cardiac hypertrophy.