RNA-seq data from A. fumigatus strain Af293 with ZfpA wild type, knockout, and overexpression
We generated this RNA-seq dataset to investigate the effects of transcription factor ZfpA (aka. AFUA_8G05010 or AFUB_082490) in the gene expression of A. fumigatus strain Af293. One of the major reasons we were interested in investigating ZfpA was because this TF had been identified as highly regulated by the oxylipins (https://pubmed.ncbi.nlm.nih.gov/33056992/). To that end, we collected RNA from spores and the mycelial tissue under three distinct conditions: (a) wild-type ZfpA, (b) ZfpA knocked out, and (c) ZfpA overexpressed. We had four biological replicates for each set of (tissue, condition) pair. Hence, we had a total of (2 tissues * 3 conditions * 4 replicates) = 24 data samples. The knockout samples could still have reads that map to ZfpA due to the fact that less than 100% of the ZfpA gene was deleted. Overall design: For the mycelial samples, RNA was extracted from the mycelial tissue 16 hours after inoculation in glucose minimal media at 37 degree celcius and 250 rpm.