Raw sequence reads of the transcriptomics for rapamycin-treated HeLa cells
Rapamycin, known for its ability to inhibit the mammalian target of rapamycin (mTOR) pathway, which is a key player in metabolism, autophagy, tumorigensis and other crucial processes. However, due to the highly complexed, transient and context-dependent nature, the molecular details on how mTOR impacts cellular processes as well as specific diseases remained largely elusive. Given that mTOR is significantly associated with lysosome both physically and functionally, we have performed a proteomic study via the CAT-Lyso method to profile lysosomal dynamics upon mTOR perturbation in HeLa cells. Here, we further present the transcriptomic profiling of HeLa cells treated with rapamycin, in order to generate a multi-omic picture for mining biological information. Overall design: To investigate the transcriptional regulations of genes, we prepared HeLa cells treated with or without rapamycin (100 nM, 24 h) and performed the bulk RNA-sequcing of the samples, followed by comparative gene expression analysis.