Growth of barcoded E. coli Nissle 1917 in different carbon sources in vitro
We generated a barcoded library of E. coli Nissle 1917. This library consists of genetically identical bacteria, except 20nt barcode integrated into lacZ locus resulting with deletion of lacZ. There are total of ~40,000 unique barcodes in this library. We grew the library in four media environments: LB, M9-Amicase-Glucose, M9-Amicase-Glycerol, and M9-Amicase-Acetaldehyde and extracted the DNA at the end of the log phase. Then we evaluated the change in the barcode frequencies compared to the T0(overnight inoculum culture) timepoint.