Variation in gene expression between two Sorghum bicolor lines differing in innate immunity response
Comparative RNA-seq analysis of MAMP triggered gene expression in two sorghum bicolor lines, BTx623 and SC155-14E, revealed a clear transcriptional response to elicitation with the microbe associated molecular pattern (MAMPs) flagellin (flg22) or chitin elicitation. Overall design: Leaf discs of the second youngest leaves of fifteen-day old Sorghum bicolor lines, BTx623 and SC155-14E, were treated with flagellin (flg22) or chitin for 1 hour. The samples [2 genotypes (BTx623 and SC155-14E), 3 treatments (mock, flg22 or chitin), 3 biological replicates] were collected and the RNAs  were extracted. Non-strand-specific RNA-seq libraries were multiplexed and sequenced using Illumina HiSeq 2000 (Illumina, Sandiego, CA).