Time course analysis of human colorectal cancer liver metastasis models under Sacituzumab Govitecan (SG) treatment or untargeted control
Human colorectal cancer organoids were injected intrasplenically to form liver metastasis in mice were treated with the TROP2-targeting antibody-drug conjugate Sacituzumab Govitecan (SG) or non-targeting control IgG1-SN-38 (ADC). Tumors were harvested at different time points: 0 h, 6 h,  24 h, 48 h, 72 h and 120 h} and subjected to single-cell RNA-seq using sample hashtag multiplexing.