RNA-seq analysis on bioactive micelle PPP8 therapy for Hemin stimulated mouse neurons
To analyze the effect of micellar PPP treatment on hemin-stimulated neurons, HT22 cells were treated with PBS, 50 µM hemin, or 25 µg/mL micellar PPP8 plus 50 µM hemin for 6 h. HT22 cells treated with PBS (control group), hemin (experimental group), or the combination of PPP8 and hemin (intervention group) were then collected for transcriptome (RNA-seq) analysis. Overall design: The total RNA was extracted through the culture of neuron cell HT22. Each group was divided into three replicates. There were three groups in total, namely, the experimental group, the control group and the intervention group.Finally, the high-throughput Transcriptome was sequenced on Illumina NovaSeq.