RNA sequencing to compare gene expression in control and CMTM6 knockdown colon cancer cells
Human colorectal cancer cell line  HCT116 was purchased from ATCC (Manassas, VA, USA). They were transduced with lentiviruses encoding control shRNA or CMTM6 shRNA. Cells were collected for RNA isolation and RNA sequencing. The goal of this study is to identify genes transcriptionally regulated by CMTM6 knockdown. Overall design: Comparative gene expression profiling analysis of colorectal cancer cells