Next Generation Sequencing Facilitates Quantitative Analysis of transcriptomes in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells sorted from the bone marrow of NSG mice transplanted with these cells
To determine what signalling pathways are affected by LILRB1 in MM cells, ARP-1 MM cell lines were transfected with lentivirus to knockdown LILRB1, injected to nsg mice, sorted from the bone marrow of NSG mice and sent for RNA-seq. Total RNAs of 2 x 10^6 CTR-KD ARP-1 cells or LILRB1-KD ARP-1 cells were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Cancer Genomics Center at The University of Texas  (Houston, TX) for RNA-seq followed by data analysis.  We use the RNA-seq data to determine differential expression of genes in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells. Overall design: Examination of gene-profiling data in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells.