RNA-seq Analysis Reveals the Anti-inflammatory Role of hnRNPF in Diabetic kidney disease
In this study, a hyperosmotic environment was simulated using 30 mM mannitol. Human renal proximal tubular epithelial (HK-2) cells were transfected to overexpress hnRNP-F, with empty vector-transfected cells serving as the negative control (NC). RNA sequencing (RNA-seq) was performed to profile transcriptomic changes, enabling comprehensive analysis of hnRNP-F overexpression-induced alterations in differentially expressed genes (DEGs) and alternative splicing events (ASEs) under hyperosmotic stress. These data were integrated with our previously acquired dataset profiling hnRNP-F-driven DEG and ASE regulation in HK-2 cells under hyperglycemic conditions. Overall design: 6 samples, 3 controls, 3 HNRNPF overexpression inHK-2 cells