Aging flu vaccine perturbation single-cell
Single-cell RNA sequencing of previously frozen peripheral blood mononuclear cells from twelve adults 65 years old or greater and twelve adults 45 years old or younger following stimulation with influenza vaccine antigens for 18 hours. We used the 10X Genomics Next GEM V2 kit and library generation protocol following the manufacturer's instructions. The generated library was analyzed on an Agilent Bioanalyzer and sequenced on an Illumina NovaSeq 6000 paired-end 150 base pairs S4 flow cell. Multiple individuals were multiplexed into a single sample, followed by genetic demultiplexing using Souporcell and HLA typing.