Gene expression profile at single cell level of human Simpson Golabi Behmel Syndrome (SGBS) and murine 3T3-L1 cells prior to and during adipogenesis
We used single cell RNA sequencing (scRNA-seq) to analyze the transcriptional diversity of cells undergoing adipogenesis using two cellular models of adipogenesis in vitro, human SGBS cells and murine 3T3-L1 cells. Overall design: SGBS and 3T3-L1 cells were isolated at two timepoints: prior to the addition of differentiation stimuli (D0) and during adipogenesis (D5 for 3T3-L1, D8 for SGBS). Live cells were isolated using Fluorescence-activated cell sorting (FACS) according to the absence of staining with propidium iodide. Next, equal numbers of SGBS and 3T3-L1 cells were mixed and analyzed using scRNA-Seq.