AE9a leukemia cells located in endosteal region exhibit a transcriptional signature enriched in hypoxia and high Ddit4 expression
Like normal HSCs, LSCs appear to reside in endosteal BM niche and utilize hypoxic niche for their stemness maintenance. To determine the molecular mechanism that regulating the stemness of LSCs, we performed RNA-seq analyses on leukemia cells isolated from collagenase treated bone (endosteal BM, EBM) and from conventional flushed BM (central BM, CBM) of AE9a leukemia mice. Overall design: Transcriptome sequencing of leukemia cells isolated from collagenase treated bone (endosteal BM,EBM) and from conventional flushed BM (central BM,CBM) of AE9a leukemia mice.