Transcriptional profiling of mammalian preimplantation embryos
We developed an adapted version of Smart-seq2, which we call Smart-seq+5', to capture both internal transcript fragments, as well as 5' transcript information, in mammalian preimplantation stages on a single-embryo level. Focussing specifically on transposable elements, we find many conserved patterns and features shared between mouse, pig, cow, rabbit and rhesus. Overall design: Transcriptional profiling using an adapted version of the Smart-seq2 protocol during preimplantation stages of mouse, pig, cow, rabbit and rhesus