Idnetification of a new DQB1*02 allele in a french  volunteer bone marrow donor.
Here we describe a novel DQB1*02 allele, identified in a a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*02:01 ,*68:01 B*44:03 ,*51:01 C*05:01 ,*16:01 DRB1*07:01 ,*14:54   DRB3*02:02 DRB4*01:01 DQA1*01:04 ,*02:01 DQB1*02:New ,*05:03 DPA1*01:03 ,*02:01          DPB1*04:01 ,*11:01 HLA-DRB1*02: New allele differs from DQB1*02 :02 by one nucleotide substitution at position 104 of exon 2 (position 213 of cDNA), where  base C is replaced by A  without chang of amini acide . In fact 39th CGC becomes CGA, both of them code for Arg.