Effect of Card9 knockout on gene expression in cardiac tissues of mice post myocardial infarction
Immune cell infiltration in response to myocyte death contributes to extracellular matrix (ECM) remodeling and scar formation after myocardial infarction (MI). Caspase-recruitment domain protein 9 (CARD9) which belongs to CARD family acts as an adapter that mediate the transduction of proinflammatory signaling cascades in innate immunity. To investigate the role of CARD9 in cardiac injury and repair post ischemia, we subjected Card9 knockout mice to myocardial infarction (MI) , and then performed RNA-seq and gene expression profiling analysis using the ischemic cardiac tissues at 3 days post-MI, to identify key genes and pathways regulated by CARD9. Overall design: 12-week-old male WT and Card9 KO mice were subjected to MI by ligation of left coronary artery. Cardiac tissues below the ligature from MI and Sham mice at 3 days post-MI were harvested and used for total RNA extraction and RNA-seq (n=2 for each group).