ATAC-seq to Profile the Chromatin Accessibility in RBM22-silencing and Control Primary Cardiomyocytes
Purpose: The purpose of this study is to identify chromatin alterations regulated by RBM22. We performed ATAC-sequencing in primary cultured neonatal mouse cardiomyocytes transfected with control siRNA or Rbm22 siRNA. Methods: Cardiomyocytes were isolated from 1-day-old C57BL/6J mice and cultured in DMEM with 10% fetal bovine serum at 37°C in an atmosphere with 5% CO2. Cardiomyocytes were stained to confirm the expression of cTnT. Cells with purity >97.5% were used for subsequent experiments. Cardiomyocytes were transfected with control siRNA or Rbm22 siRNA for 48 hours. Results: We identified regions of chromatin that were altered between cardiomyocytes transfected with the indicated siRNAs. Overall design: Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) was performed to profile chromatin accessibility in primary cultured neonatal mouse cardiomyocytes transfected with control siRNA or Rbm22 siRNA.