Enhanced  rumen microbiome analysis using viability PCR to identify genuine microbial biomarkers
Our study aimed to examine the effects of PMA on the rumen microbial community using 16S rRNA gene sequencing during typical in vitro experiments. These samples were collected from five Hannwoo cows before morning feeding. The samples are divided into two treatment (CON and PMA) groups added three time groups (0h, 24h, 48h): CON_0h, CON_24h, CON_48h, PMA_0h, PMA_24h, PMA_48h.