Epigenetic selectivity of TopoIIa redistribution and histone eviction of anthracyclines
Anthracyclines act by disrupting the interface of TopoII and DNA and by evicting histones. The anthracycline-specific redistribution of TopoII and its association with histone eviction were addressed in K562 cells. Chromatin immunoprecipitation, followed by deep sequencing (ChIP-seq) against endogenously tagged TopoIIa, and transposase-accessible chromatin with sequencing (ATAC-seq) was performed 4 hours after anthracycline exposure. Overall design: For ChIP-seq: Endogenous tagged 3×Flag-TopoIIa K562 cells were treated with 10 µM of indicated drug for 4 hours. Then cells were fixed in 1% formaldehyde and processed as dessscribed in PMID 19275939, 25961671 for ChIP against anti-Flag M2 (F3165, Sigma) . For ATAC-seq: K562 cells were treated with 10 µM of indicated drug for 4 hours. Then cells were processed as desscribed in PMID 24097267 and 28846090.