Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients
Plate-based scRNA-seq data of (1) GFP possitive cells sorted from small intestines of ChAT-eGFP mice [in vivo], and (2) fluorescent cell populations sorted from tuft type-specific small intestinal reporter organoids [in vitro]. Overall design: (1) GFP+EpCAM+DAPI- cells from 3 ChAT-eGFP mice (pooled) were isolated from small intestinal cell suspensions by fluorescence associated cell sorting and subjected to plate-based single-cell RNA sequencing. (2) Fluorescent populations (GFP+/-; mScarlet-I+/-; DAPI-) from tuft type-specific reporter organoids (ChAT-eGFP;Nrep-mScarlet-I; ChAT-eGFP;Gng13-mScarlet-I; ChAT-eGFP;Folr1-mScarlet-I) cultured in 3 different medium conditions (ENR; ENR+IL4+IL13[crypt]; IL4+IL13+BMP2+BMP4+WNT5a+IL25+NRG1+Acetylcholine[villus]) were isolated by fluorescence associated cell sorting and subjected to plate-based single-cell RNA sequencing. Raw and processed data, including Seurat objects (RDS files), are provided.