Effect of tRF-1-Ser on the gene expression of breast cancer cell MDA-MB-453
tRNA-derived small RNAs (tsRNA) are a new type of noncoding RNAs that can be mainly classified into two groups: tRFs (tRNA-derived fragments) and tiRNAs (tRNA halves). The abnormal expression of tsRNAs is known to play an important role in the biological progression of breast cancer. However, it's still unclear about the mechanism of tsRNAs in cancer. tRF-1-Ser is a tRF that is high expression in breast cancer and negatively regulated by 25(OH)D. Our study aims to find out the effect of tRF-1-Ser on breast cancer and explore the change of RNA in tRF-1-Ser knock-down breast cancer cells. Overall design: To explore the influence of tRF-1-Ser on breast cancer, we transfected MDA-MB-453 with shtRF-1-Ser by lentivirus and verified the efficiency of knock-down by qPCR. The groups that MDA-MB-453 transfected with shtRF-1-Ser or shNC are named lv_shtRF or lv_con. Each group was duplicated three times.