singlecell RNA sequencing of lung transplanted mice
This dataset contains single-cell RNA sequencing (scRNA-seq) data from murine lung transplantation models. The study investigates the expression of fibroblast activation protein (FAP) as an early biomarker in chronic lung allograft dysfunction (CLAD). Lung samples were collected at baseline, day 7, and day 28 post-transplantation to analyze gene expression patterns associated with fibrotic remodeling. This dataset provides insights into the cellular heterogeneity and dynamic changes occurring in lung allografts during CLAD progression. Overall design: C57BL/6 mouse lungs were orthotopically transplanted into C57BL/10 recipients. Lung tissues were harvested at three time points: baseline (resting), day 7, and day 28 post-transplantation. Single-cell suspensions were prepared and processed using the 10x Genomics platform. Raw sequencing data were processed using Cell Ranger, and subsequent data analysis was performed with Seurat for clustering, UMAP visualization, and differential gene expression analysis. This experimental design allows for temporal assessment of gene expression changes during CLAD development.