RNA-seq and ribosome profiling (ribo-seq) of thermo-sensitive arf1-11 mutant and arf1-WT S. cerevisiae strains
Arf1 hyperactive mutant strain arf1-11 was used to investigate changes induced by Arf1 activity in the transcriptome and translatome. WT and arf1-11 strains were shifted for 30 min to 37C before preparation for RNA and ribosomes purification.