effect of depletion of Znf143 on gene expression of C2C12 myoblasts with or without induction for myogenic differentiation
In adults, muscle stem cells (muscle satellite cells, MuSCs) can be recruited to supply myoblasts for routine muscle fibre homeostasis or muscle regeneration. Epigenetic regulation is essential for the proliferation and differentiation of muscle stem cells and myoblasts. We here report that Znf143 is critical for proliferation and differentiation of myoblasts. Znf143 depletion in C2C12 myoblasts dramatically repressed their myogenic differentiation, impairing multiple biological processes including striated muscle contraction, muscle cell differentiation, and myofibril assembly. This is consistent with the limited regeneration of skeletal muscle after freeze injury in mice with MuSC-specific Znf143 ablation. Overall design: C2C12 myoblasts were transfected with siRNA of non-targeting control or Znf143, then left untreated or induced for myogenic differentiation for 5 days. The resultant cells were then harvested for RNA-seq analysis.