Genetic Variants of Phospholipase C-?2 Confer Altered Microglial Phenotypes and Differential Risk for Alzheimer's Disease [snRNA-seq]
The PLCG2 variants bidirectionally modulate disease pathology by inducing distinct transcriptional programs that identify microglial subpopulations associated with protective or detrimental phenotypes Overall design: Nuclei from 7.5-month-old 5xFAD, 5xFAD;M28L, and 5xFAD;P522R mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of NeuN and analyzed using scRNAseq.