Effect of NEK2 deficiency on gene expression of CD19-selected spleen B cells of Eµ-myc mouse model
NEK2 overexpression in the B cell lineage affects B cell development. To determine whether deficiency of NEK2 inhibits B cell malignancies, we crossed Nek2 knockout mice with Eµ-myc transgenic mice, a well-established Myc-driven preclinical model for B cell malignancies. Three genotyping offsprings were obtained: Eµ-myc/Nek2+/+ (NEK2 wild type), Eµ-myc/Nek2+/- (NEK2 heterozygotes), and Eµ-myc/Nek2-/- (NEK2 homozygotes). Bulk RNA-seq was performed on CD19-selected spleen B cells to view the transcriptome differences. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CD19-selected spleen B cells from 6-8 weeks old Eµ-myc mice with indicated NEK2 deficiency.