Multiome analysis (ATAC + Gene Expression (GEX)) of SUN1 knockout and control CT26 tumors grown in syngeneic mouse model
T cell infiltration is essential for immune checkpoint inhibitors to be effective in treating solid cancers. Through a bioinformatic pipeline, we identified a target gene SUN1 that might relate to modulating immune cell infiltration and immune response. Thus, we generated one Sun1_knockout CT26 cell line (Sun1_KO) using CRISPR-Cas9. By performing multiome single nuclei sequencing using tumors grown in syngeneic model, we set out to understand how mouse Sun1 can affect the regulatory network in tumor cells in vivo. Overall design: Sun1_KO and Sun1_WT cells were subcutaneously injected in the right flank. Tumors were collected at day 14 post-inoculation, and digested into single cells, which were applied to the preparation of 10x multiome libraries according to the instruction. The resulted GEX library and ATAC library were sequenced separately.