Arabidopsis thaliana gene expression changes upon chitosan and CAPE treatments
The phytopathological studies often deal with approaches involving the employment of high elicitor concentrations necessary to provoke responses that could be easily observed and quantified. We tested the range of gene expression changes for a low concentration chitosan elicitor treatment of seedlings, 0.001%, and we compared it with the higher one, 0.01%. We uncover both the quantitative and qualitative changes in gene expressions between the two treatments. Further on, we tested if the gene expression can be modified by the treatment with the defence-related CAPE peptide (10 uM). We discovered several interesting candidate genes specific for each of treatments, and combination of treatments, that would be worth of further more focused genetic studies. Overall design: Comparative gene expression profiling analysis of RNA-seq data from A. thaliana seedlings, treated for 24 h with the two different chitosan elicitor concentrations (0.001% and 0.01%), CAPE peptide derived from AtPR1 (10 uM), and combination of both. The seven-day-old whole seedlings (ca 50 seedlings) were used as a source of RNA, always as three replicates.