RNA-seq Analysis of Soluble Tim-3 in CD8+ T Cells
OT-I CTLs were pretreated with sTim-3 protein, followed by stimulation with anti-CD3 and anti-CD28 antibodies. Then cells were harvested and snap-frozen immediately in TRIzol, awaiting RNA-seq analysis. Following the manufacturer protocol, NEBNextUltra RNA Library Prep Kit for Illumina was used to generate the sequencing libraries before adding the index codes to attribute sequences to each sample.