Triticum aestivum Transcriptome or Gene expression
Abstract: (1) background: float wheat is the immature fruit of wheat. It is a commonly used traditional Chinese medicine in clinic. It has different effects at different stages, especially in the treatment of hyperhidrosis. (2) Methods: five growth stages of float wheat were analyzed by UPLC-MS/MS and RNA sequencing of gene expression. Metabolome and transcriptome analysis were used to explore the formation mechanism of float wheat and metmap was used to analyze the pharmacodynamic material basis. (3) Results: different metabolic product accumulation and gene expression patterns were observed, especially in phenylpropane biosynthesis pathway and flavone and flavonol biosynthesis pathway. The main findings include: stage D to e is the harvest time of the main metabolites, multiple key regulatory genes (tapal, taexp1, tacipk, tanac2, tagst, tasut, talox, tahsp70, tamyb, tapod, tasos1, novel.3099, taerf, tacat, tappo, tagrp, tapr1, Talea, tapip, tawrky, tacomt, tafro, Tachi, tagid1) and possible pharmacodynamic substances (phenylpropanoid biosynthetic pathway and flavonoid biosynthetic pathway metabolites)metabolites such as 4-cinnamoyl-mussatioside, luteolin-11-methyl ester, coumaric acid-o-glucoside, n- (2,4-dinitrophenyl) - DL methionine sulfoxide, 2-feruloyl-1-sinaoyl gentian disaccharide, ethyl salicylate and complex flavone oxane hybrid, luteolin-11-methyl ester. (4) Conclusion: This study preliminarily explored the formation mechanism of floating wheat, defined the harvest time of floating wheat, and provided an important theoretical basis for its pharmacodynamic material basis.Key words: float wheat; Metabonomics; Transcriptomics; Phenylpropane biosynthesis; Biosynthesis of flavonoids and flavonol; WGCNA