Dynamic molecular signatures of acute myocardial infarction based on transcriptomics
Peripheral blood mononuclear cells were collected from the patients. RNA-seq and analysis was performed on the collected samples. Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer's instructions(Invitrogen) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined using 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). High-quality RNA sample (OD260/280=1.8~2.2, OD260/230=2.0, RIN=6.5, 28S:18S=1.0, >10µg) is used to construct sequencing library Overall design: We enrolled patients with AMI aged between 30 and 70. RNA-seq analysis was performed on the peripheral blood mononuclear cells collected from the patients at three time points: 1 day, 7 days, and 3 months after AMI. Differential genes and metabolites between groups were screened by bio-informatics methods to understand the dynamic changes of AMI in different periods.