Impacts of Glut1 Deficiency and High Glucose Exposure on Transcriptomic Profiling in Human Brain Microvascular Endothelial Cells
We used RNA-seq analysis to determine the alteration of transcriptomic profiling in human brain microvascular endothelial cells Overall design: We utilized validated siRNA to suppress Glut1 expression and applied high glucose treatment in human brain microvascular endothelial cells. Following treatment, we isolated RNA to analyze changes in transcriptomic profiles via RNA sequencing