RNA-seq of in vitro radio-resistant human GBM cell line (LN229) and patient-derived culture (PS1) post YBX3 knockdown
Glioblastoma (GBM)is the most aggressive brain tumor with poor overall survival due to high recurrence rate. The recurrent GBM tumors are highly aggressive and resistant to therapy. In the current study, we explored the potential role of Y-box binding protein 3 (YBX3) in glioblastoma aggressiveness. We found significantly high expression of YBX3 in GBM compared to low-grade glioma, and the expression of YBX3 significantly correlated with poor overall and disease-free survival of GBM patients. Knockdown of YBX3 in established GBM primary cultures and cell line abrogated the invasion and migration of GBM cells in vitro, and tumorigenicity and metastasis in vivo. Further, we found high expression of YBX3 in the recurrent GBM patient biopsies. To decipher the genes and biological pathways by which YBX3 regulates the GBM aggressiveness, we performed RNA-Seq experiment on LN229-Relapse and patient-derived primary culture relapse cells (PS1) after stable knockdown of YBX3.