RNA-Sequencing Reveals the Changes of Gene Expression Profile in the Lipopolysaccharide-induced Immunoresponsed Uterine Body after the Immunosuppressant Cyclosporine A-treatment
Infection of Gram-negative bacteria in female genital tract causes systematic immune-response and reproductive dysfunction. Immunosuppressor Cyclosporine A (CsA) is widely used in the treatment of genital tract immune response, however, the knowledge on the changes of transcriptome after CsA inhibiting lipopolysaccharide (LPS)-induced immune response still remains unknown. Overall design: Twelve New Zealand white rabbits (n=12) were randomly divided into 4 groups. The rabbits in the control group were treated with normal saline, and CsA solvent was intramuscularly injected. The rabbits from LPS group were treated with LPS by intrauterine infusion, and 500 µL of CsA solvent was intramuscularly injected. In the CsA treatment group, the uterus was perfused with normal saline, intramuscularly injected with CsA; The rabbits of LPS and CsA co-treatment group (LPS+CsA) were treated with LPS by intrauterine infusion and CsA by intramuscular injection. After 3 hours of treatment, collect uterine body tissue, that subsequently rapidly frozen by liquid nitrogen and then stored at -80?.