Transcriptomic analysis of PNAd+ HEV-like vessel in the skin
Peripheral node addressin (PNAd)-positive high endothelial venule (HEV)-like vessels manifest in diverse non-lymphoid peripheral tissues under varying pathophysiological conditions, encompassing the affected skin in atopic dermatitis (AD). Nonetheless, the characteristics of HEV-like vessels in the inflammatory skin, including gene expression profiles and formation mechanisms, have not been subjected to thorough evaluation. In this study, we characterized these aspects of PNAd+ HEV-like vessels in the inflammatory skin using a mouse AD model induced by repeated topical applications of a vitamin D3 analog, MC903. We performed RNA sequencing analysis of PNAd positive and negative blood endothelial cells (BECs) in AD-like skin (AD PNAd+ BECs and AD PNAd- BECs), NT skin (NT PNAd- BECs), and NT LNs (LN HEV).