WT and Ccr2-/- small intestinal macrophages
To better determine heterogeneity of small intestinal macrophages, CD64+ macrophages were sorted for single cell RNA sequencing Overall design: Live CD45+Lin-(CD3,TCRb,B220,SiglecF,Ly6C,Ly6G)CD11b+MHCII+CD64+ cells were FACS sorted from 3-4 pooled mouse small intestines and loaded on to the 10X Genomics single cell platform for subsequent sequencing