Effect of abemaiclicb and deletion of TP53 on gene expression in MCF7 cells at different timepoints
CDK4/6 inhibitor, abemaciclib induced cell cycle arrest and senescence in breast cancer. We reported that deletion of TP53 prevented the senescence features. In this study, we knockout TP53 by CRISPR Cas9 system and treated MCF7 cells and p53 knockout cells with abemaciclib for up to 29 days to identify the difference of senescence associated secretary phenotype genes and cell cycle related genes. features. Overall design: We established TP53 knockout (p53KO) cell line by CRISPR Cas9 system in MCF7 breast cancer cell line. Cells were treated with 50 nM abemaciclib. We collected cells on day 0 (no treatment), day1, day 3, day 14 and day 21. We then performed gene expression profiling analysis from RNA sequencing of parental MCF7 and p53KO cell.