Search for DNA methylation regions associated with seasonal plasticity of gut length in medaka (Oryzias latipes)
This study aims to identify DNA methylation regions and sites associated with seasonal plasticity of gut length in wild medaka in Japanese archipelago. To achieve this goal, we performed whole-genome bisulfite sequencing (WGBS) and targeted bisulfite sequencing. First, we extracted DNA from the gut of medaka sampled in winter and summer in Kagawa Prefecture and comprehensively identified regions where DNA methylation fluctuates in correlation with the seasons by the WGBS. Next, we performed PCR-based targeted bisulfite sequencing for the identified region to determine which CpG sites showed DNA methylation correlated with the season.