Sequencing of Vibriophage with and without the BREX-inhibitor OrbA infecting V. cholera encoding the SXT VchInd5
Total DNA was collected from infected cultures and used to generate DNA libraries. DNA libraries were prepared at SeqCenter (Pittsburgh, PA) using the Illumina DNA Prep kit and IDT 10bp UDI indices, and paired-end sequencing (2x150 bp) was performed on an Illumina NextSeq2000 or Illumina NovaSeq 6000 (SeqCenter, Pittsburg, PA).