5mC and 5hmC detection by nanopore sequencing
5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles in regulating gene expression. Using whole genomic DNA from mouse cerebellum, we have benchmarked 5mC and 5hmC detection by Oxford Nanopore Technologies sequencing against widespread orthologous techniques. In addition, we demonstrate the direct detection of these modifications within an immunoprecipitated DNA sample, and study strand-specific asymmetrical modification and their effect on transcription factor binding using new duplex sequence reads.