Analysis of differential gene expression in NSCLC cancer cell with or without EGFR-TKI resistance
We have established a Genfitnib resistant cell line, Ire, from a NSCLC cell line, PE089, with deletion of exon 19 in EGFR. RNA-sequencing analysis was performed to analyze the diffreential gene expression between thes two cell lines Overall design: Total RNA was extracted from PE089 or Ire cell and the differential gene expression was determined by using RNA-sequencing analysis