Identification of a new B*57:01 allele in french  volunteer bone marrow donor
Here we describe a novel B*57 :01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*01:01 ,*66:01 B*49:01 B*57:01New C*07:01 ,*-  DRB1*07:01 ,*11:01 DRB3*02:02 DRB4*01:03N DQA1*02:01 ,*05:05 DQB1*03:01, *03:03 DPA1*01:03 ,*01:03 DPB1*04:01, *04:01 HLA-B**57: 01New allele differs from B*57 :01 :01 :01 by one nucleotide substitution at position  30 of exon 5 (position 925 of cDNA), where  base G is replaced by A  inducing Met instead Val (GTG>ATG) .