Transcriptome-wide identification of ILF3 binding RNAs by RIP-seq.
Interleukin-3 (ILF3), particularly in macrophages. We identified the mRNA targets of the ILF3 mRNA-binding proteins using RNA immunoprecipitation and sequencing (RIP-seq). We performed RIP of ILF3, and the associated mRNAs were identified through next-generation sequencing. More than 5000 transcripts, including both protein-coding and non-coding transcripts, were identified from each RIP-seq sample.