RNA-seq of ME-HESC in SRF-deficient vs Normal Conditions
The transcription factor SRF plays known roles in mitogenic cell response and muscle cell development, and potential roles in endometrial stromal decidualization have been reported. After RNAi-based SRF knockdown in menstrual effluent-derived primary human endometrial stromal cells (ME-HESC), SRF-deficient cells exhibited disrupted cytoskeletal remodeling, cell cycle, and extracellular matrix genes. These data show that SRF is important for endometrial stromal cell homeostasis. Overall design: Primary menstrual effluent-derived human endometrial stromal cells (ME-HESCs) were obtained from deidentified archives that were previously banked after non-invasive isolation from menstrual fluids of healthy volunteers with the NIEHS Clinical Research Unit according to IRB approved, published methods and analyzed by RNA-seq at passage number 6. Cells from three distinct human donors were treated with control non-targeting siRNA or siSRF to analyze the impact of SRF deficiency on the transcriptome.