Transcriptome Sequencing for Genome Annotation and Expression Atlas of Jack Bean (Canavalia ensiformis)
Multiple organs of jack bean (Canavalia ensiformis) were subjected to RNA sequencing-based transcriptome analysis using the illumina platform.Total RNA was isolated using TRIzol reagent following manufacture's procedure. mRNA was enriched from the Total RNA using poly A selection module. The enriched mRNA was fragmented using enzymatic methods to match the expected insert size. The fragmented DNA was ligated with Illumina-compatible Adapter. PCR was conducted to amplify the library and to generate the full-length adaptor containig unique index sequence in each library samples. The quality and quantity of library samples were determine using Tape Station, and qPCR. The library samples were proceeded to sequencing with 300 cycle (150-PE) using Illumina NextSeq 2000. The demultiplexing and Fastq generation was performed using Illumina DRAGEN BCL Convert - 4.2.7 of NextSeq 2000 Instrument.