Bulk RNA-seq of adult pig and rat central canal–associated spinal cord cells
Bulk RNA sequencing was performed on central canal–associated spinal cord cells isolated from adult pig (Sus scrofa) and rat (Rattus norvegicus) spinal cords and expanded under ex vivo culture conditions. Libraries were sequenced on the Illumina NovaSeq 6000 platform using 150 bp single-end reads. Transcript abundances were quantified using Salmon (v1.x) against species-specific reference transcriptomes (pig: ss11; rat: rn6). This dataset enables cross-species analysis of transcriptional programs associated with proliferative activation and progenitor-associated states in the mammalian spinal cord. Overall design: Central canal–associated regions were microdissected from adult pig and rat spinal cords. Cells were expanded as adherent cultures under defined serum-free conditions. Total RNA was extracted and strand-specific poly(A)+ libraries were prepared using the Illumina TruSeq Stranded mRNA workflow. Libraries were sequenced on an Illumina NovaSeq 6000 (150 bp, single-end). Transcript quantification was performed with Salmon (v1.x) using ENSEMBL/ENCODE v104 transcript annotations. Raw FASTQ files and per-sample transcript-level quantifications (quant.sf) are provided.