Effect of methanol fixation on single cell RNA sequencing of the murine dentate gyrus (droplet-based scRNAseq).
We evaluated the effect of methanol (MeOH) fixation on adult murine dentate gyrus (DG) single cell suspensions processed with droplet-based scRNA-seq. Overall design: Methanol-fixed neuronal and non-neuronal cells from the DG of adult C57BL/6J mice were isolated and proccessed with 10x Genomics Chromium controller, kit 3' v3.1 to perform library construction.