RNA-seq of the brain and spinal cord of NTDs fetal mice at day 9.5
To investigate the genes exprsssion change  in the brain and spine at E9.5 days in the folate-deficient NTDs mouse model, we established NTDs mouse model and normal mouse model. Overall design: Male and female CD-1 mice were fed on low-folate diet for more than 8 weeks. Sexually matured individuals were mated overnight; vaginal plug was detected at 8:00 am in the following morning and was designated as E0.5 day. NTDs mouse models were  induced by intraperitoneal injection with 1.5 mg/kg of MTX (Sigma–Aldrich, USA) on E7.5. On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope. All samples with NTDs clinical manifestations were collected for experiments.We then performed gene expression profiling using data from four different RNA-seq.