Whole Genome Sequence of dye degrading Priestia megaterium strain AKSKSLAB05
Genomic DNA samples were purified using 1X AMPure XP beads (A63880, Beckman Coulter, USA) to remove residual impurities. The purified DNA was end-repaired and A-tailed using the NEBNext Ultra II End Repair dA-Tailing Module (E7546, New England Biolabs, MA, USA), followed by another cleanup with 1X AMPure XP beads. Native barcode ligation was carried out using the Blunt TA Ligase Master Mix (M0367, New England Biolabs, MA, USA) with barcodes supplied in the SQK NBD114.96 kit (Oxford Nanopore Technologies, UK). The barcoded samples were again cleaned with 1X AMPure XP beads and quantified using the Qubit dsDNA HS Assay Kit (Q32851, Thermo Fisher Scientific, USA) on a Qubit 4 Fluorometer (Q33238, Thermo Fisher Scientific, USA).Barcoded DNA libraries were ligated to sequencing adapters from the SQK-NBD114 kit (Oxford Nanopore Technologies, UK) using the NEBNext Quick Ligation Module (E6056, New England Biolabs, MA, USA). The final libraries were quantified with the Qubit 4 Fluorometer and sequenced on the Oxford Nanopore PromethION24 platform using a PromethION flow cell (FLO PRO114M) and associated Data Acquisition Unit, according to the desired library concentration and sequencing throughput.Authors: Arshiya Khan (Department of Bioinformatics, Alagappa University, Karaikudi 630003, Tamil Nadu, India); Dr. Anuraj Nayarisseri (In silico Research Laboratory, Eminent Biosciences - EMBS, Indore 452010, Madhya Pradesh, India); Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi 630003, Tamil Nadu, India).