Saccharomyces cerevisiae BY4741 RNA-seq Raw sequence reads
Biosorption of lanthanides is important in recycling of the value-added materials. Previously, we analyzed the absorption of the dysprosium (Dy) in screening strains of the unpopular yeast species Schizoblastosporion sp. However, it is necessary to use the well-known yeast Saccharomyces cerevisiae to make an easy-to-breed and efficient Dy-absorbing strain. Thus, we analyzed the physiological response and gene regulation of S. cerevisiae under Dy-absorbing conditions.As a results, approximately 40% of the Dy was located in the cell wall fraction, and the remaining 60% was located in the intracellular fraction. The qRT-PCR analysis showed that the expression of four genes, NCW2, PIR1, CRH1, and OLE1, was unregulated, and that of ATP14 was down regulated. These results suggest that cell wall rearrangement was caused by NCW2, PIR1, and CRH1; the repair of the oxidative damage to the membrane lipid was responded by OLE1; and the intracellular oxidation caused by the imperfect ATP14 product.