Characterization and Analysis of Whole Transcriptome of Ningxiang Pig Spleens Reveal Potential Mechanism for Transcriptional Regulation in Immunity [miRNA-Seq]
we comprehensively analyzed the whole transcriptome of the spleen from three important developmental nodes(30d, 90d and 210d) of Ningxiang pig (a Chinese indigenous breed). A total of 812, 364 and 865 differentially expressed (DE) mRNAs, 98, 37 and 97 DE miRNAs, 278, 220 and 271 DE lncRNAs, and 113, 96 and 96 DE circRNAs were identified between 90d and 30d, 210d and 30d, and 210d and 90d, respectively. And their molecular characterization, spatio temporal expression pattern, potential function and interaction with miRNAs were analyzed. Additionally, co-expression network analysis identified the potential non coding RNAs(ncRNAs) related to immunomodulation, e.g., ssc-miRNA-150, ssc-miRNA-497, MSTRG.24160, MSTRG.18646. Furthermore, our results also revealed that miRNAs and circRNAs may have evolved to regulate a large set of biological processes of Ningxiang pigs spleens, and circRNAs may play the role of miRNA sponges. Our study provides new insights into the expression changes of RNAs during the spleen development process of Chinese indigenous breed Ningxiang pigs, which might contribute to the phenotypic formation of immune function in Chinese native pig breeds. These results greatly improved our understanding of the molecular mechanisms which regulate the immune function of the spleen of Ningxiang pigs. Overall design: we comprehensively analyzed the whole transcriptome of the spleen from three important developmental nodes(30d, 90d and 210d) of Ningxiang pig (a Chinese indigenous breed)by using Illumina Hiseq 4000.