Relative MicroRNA expresssion profile of porcine embryonic and somatic cells
MicroRNAs (miRNAs) have been revealed to control the amount of mRNAs. miRNAs were known to be involved in the derivation and maintenance of pluripotent cells. However, the miRNA profile of pig pluripotent cells has not been defined well. Here, we report expression profiles of pluripotent and somatic (or differentiated) cells in pig to discover cell type-specific miRNAs for pluripotent and differentiated cells. Two blastocysts (BL) samples and embryonic stem cell-like (ES-like) cells were used for pluripotent cells. We selected embryoid bodies (EB) and fetal fibroblasts (PFF) as somatic cells. miRNAs were extracted from samples, then libraries were prepared to analyze miRNAs. We classified miRNAs into two categories, known and novel miRNAs. 72, 28, 19, 39 known miRNAs were relatively high in BL, ES-like, EB, and PFF, respectively. Also, expression levels of 15 and 22 known miRNAs were relatively high in pluripotent and differentiated cells. miRNAs that are well-known to control pluripotency did not found in our data. However, the list of cell type-specific miRNAs can be utilized to induce or maintain pluripotency in further studies. Therefore, our work will promote the application of miRNAs on pluripotency study in the pig. Overall design: MicroRNA expression profiling of two PA blastocyst samples and three cell line samples (pig ES-like cells, Ebmryoid body from ES-like cells, pig fetal fibroblasts)