Effects of 4-HDHA on gene expression in colonic tissue of colitis mice
To investigate the roles of 4-HDHA in the regulation of cell function and gene expression of colonic tissue, we treated colitis mice with 4-HDHA intravenously. Then, we performed gene expression profiling analysis using data obtained from RNA-seq of colonic tissue of colitis mice treated with or without 4-HDHA. Overall design: Comparative gene expression profiling analysis of RNA-seq data for colons from adult C57BL/6 mice that were treated with 3% DSS continuously for 7 days. The mice were divided into two groups:one group was treated with PBS( 200ul/20g/day ) intravenously and the other group was treated with 5um 4-HDHA (diluted in PBS,200ul/20g/day) intravenously .The injection was along with 3% DSS for 7 days.