Genome wide analysis of binding regions for MYC, MYCN and various histone modifications using Cut&Run
Cut&Run analysis was performed in RPMI-8402 cells to analyze binding regions of MYC, MYCN and various histone modifications in normal culture conditions Overall design: RPMI-8402 cells were incubated with antibodies specific to MYC(CST, #5605), MYCN(CST, #84406), H3K4me3 (Epicypher, 13-0041), H3K27ac (Abcam, ab4729), H3K4me1 (Abcam, ab176877) or control IgG (CST, #2729) overnight and subsequently with CUTANA pA/G-Mnase to release cleaved chromatin fragments. Chromtain fragments were then purified utilizing with DNA purification kit. Libraries were generated using a NEBNext Ultra II DNA library prep kit and sequencing was performed on Illumina NovaSeq at GENEWIZ Azenta Life Sciences Co. Ltd.