The transcription factor IRF4 regulates the differentiation and function of intestinal ILC3s
To investigate the role of IRF4 in the regulation of ILC3 development and function, we used the wildtype mice as control and carried out single-cell RNA sequencing  (BD Rhapsody) on  gut ILC3s. Overall design: ILC3s from Irf4flox/floxRorccre and Irf4flox/flox  mice were isolated from the small intestine and analyzed by cleavage under targets and tagmentation (CUT&Tag).