Single-cell RNA sequencing of Hyaluronan Mediated Motility Receptor (Hmmr) overexpressing mice after cardiac ischemia.
The goal of this experiment was to profile the cellular response to myocardial infarction in mice overexpressing (OE) the receptor of hyaluronan-mediated motility (RHAMM, also known as HMMR). Cardiac interstitial cells were isolated from WT and Hmmr-OE mice, 3 and 7 days after cardiac ischemia with reperfusion. Single, living cells were sorted by FACS and used as input for single-cell RNA sequencing using the 10X Genomics Chromium platform.