Single molecule analysis reveals functionally relevant isoforms of telomerase RNA in moss
The goal of the study is to determine the secondary structure of telomerase RNA in P. patens in vivo (using DMS-Seq) or in vitro (using SHAPE-Seq). cDNA with induced mutations was generated with TGIRT-II or Superscript-II in the presence of MnCl2 (SHAPE-Seq). Samples for sequencing were prepared in biological triplicates.