Single-nuclei RNA-seq of flowering plants
We conducted single-nuclei transcriptome profiling of multiple tissues from a range of flowering plant species. The resulting snRNA atlases were used to track the activity of hundreds of transcription factors (TFs) across species and celltypes, by leveraging genome-wide TF-target gene data from accompanying multi-species DAP-seq experiments (DAP-seq data available as SRA BioProject PRJNA1177505). The final integrated dataset revealed clear patterns of regulatory conservation across the plant phylogeny, as well as key examples of regulatory re-wiring to enable new cell types and lineage-specific adaptations.