RNA-seq transcriptomics and DIA proteomics of OX-ZmPDI, CR-OsPDI  transgenic plants in rice
As one of the most salt-tolerant grasses, mining and mechanism analysis of salt tolerance gene resources from Zoysia japonica Zoysia matrella [L.] Merr. can not only board the theoretical information of salt tolerance, but also provide new salt-resistant genetic resources for crop breeding. The salt-inducible protein disulfide isomerase (ZmPDI) of Zoysia matrella [L.] Merr. was proved to enhance the salt tolerance in homologous overexpression transgenic plants. In order to value its potential implication in crop, we carried out the salt tolerance valuation in heterologous overexpression transgenic rice (OX-ZmPDI), Wild-type (WT) and LOC_Os11g09280 (homologous gene OsPDI) knock-out rice CR-OsPDI by CRISPR-Cas9 system (CR-ZmPDI), and found that ZmPDI can enhance the salt tolerance in rice significantly, whereas loss-of-function of OsPDI reduced the salt tolerance. Collected roots of OX-ZmPDI transgenic, CR-OsPDI transgenic and wild-type (WT) plants at 0 and 24 h after salt treatment were conducted for RNA-seq and data-independent acquisition (DIA) proteome sequencing. Combined analysis of the transcriptome and proteome revealed that ZmPDI may enhance the salt tolerance of rice by modulating laccase-6, zingipain-2, barwin, FKBP46, ARK4C10, GBSSII, Pho1 and TRXf1. The results provided new information for the molecular regulation mechanism of ZmPDI improving salt tolerance, and prove the potential of ZmPDI for application in crop breeding.