Mouse gut microbiome sequences
C57Bl/6 mice (Jackson Labs) were fed conventional or high tryptophan diet for 7 d, or orally gavaged with DMSO (vehicle) or tryptophan metabolites (indole-3-aldehyde, I3A; indole-3-pyruvic acid, IPyA; or tryptophol, IEt) for 2 d. Murine gut microbiome (fecal) samples were subjected to next-generation sequencing (16S rRNA gene analysis) to determine microbial composition. Samples were paired end sequenced (2x250bp) on an Illumina MiSeq. Number of samples: 80 samples from 16 individual cages of co-housed female mice, 5 mice per cage, 10 mice per treatment group. Scott, S.A., Fu, J. & Chang, P.V. Dopamine receptor D2 confers colonization resistance via microbial metabolites. Nature (2024). https://doi.org/10.1038/s41586-024-07179-5