MiRNAs and proteomic profiling of T. gondii sEVs (PRJCA039734)
Models of T. gondii RH strain co-cultured with cervix adenocarcinoma cells (HeLa) and human normal cervical epithelial cells (H8) were applied respectively in vitro. T. gondii tachyzoites were purified and continuous cultured in vitro for sEVs ultracentrifugation isolation. Both T. g-derived and host-derived RNAs and proteins might be cargoed by sEVs of T. gondii after cultured with host cells feeder layer in vitro. Analysis of sEVs miRNAs and proteins helps to screen out bioactive ingredients with anti-tumor potentials and further investigate its underlying molecular mechanisms of apoptosis induction and cell cycle arrest.