A novel simultaneous short-course nitrification, denitrification and fermentation process: bio-enhanced phenol degradation and denitrification in a single reactor
To further analyze the metabolic mechanisms and interactions between functional bacteria in the SNDF system, activated sludge was sampled on day 156 and analyzed using metagenomic sequencing. DNA integrity was detected by gel electrophoresis. DNA extracts were fragmented (~400 bp) using Covaris M220 (Genedenovo Biotechnology, Guangzhou, China) to generate paired-end libraries. Samples were sequenced by Illumina NovaSeq/Hiseq Xten(Illumina, USA) platform (Guangzhou, China). 16sRNA sequencing was used to determine changes in microbial community structure.