Differential gene expression in HCC cell lines Huh7 and PLC under high and low glucose culturement
To gain a deeper understanding of the mechanism by lncRNAs involved in glucose metabolism during the progression of HCC, we conducted transcriptome sequencing on HCC cells treated with low (5 mM) and high (25 mM)  glucose and carried out analyses for the lncRNAs with a significant change of expression combined with TCGA-LIHC data. Huh7 and PLC cells were treated with low glucose (5mM) or high glucose (25mM) for 48 h, whole-transcriptome sequencing (RNA-Seq) was performed to identify the significant changed lncRNAs with glucose. Overall design: To investigate the role of glucose-related lncRNAs in HCC,we performed an RNA-sequencing analysis with Huh7 and PLC cells cultured in low glucose (5 mM) and high glucose (25 mM) for 48 h.Comparative gene expression profiling analysis of RNA-seq data for high glucose and low glucose treatment for 48 h in Huh7 and PLC cells.