Glycogen deficiency impairs diurnal energy metabolism and cell division in Synechocystis
Study on how two glycogen deficient Synechocystis sp. PCC 6803 mutant lines (glgC-1 and glgC-6) respond during a light dark cycle compared to the wildtype. Cells were pre-cultivated in shake flasks under constant light. For the main experiment, 2 cultures of each strain were grown in a multi-cultivator. After an acclimation to the conditions in the multi-cultivator (standard conditions) of 24 hours of light followed by 12 hours of darkness the experiment was started. After 11 hours of light, the first samples for end of day (EoD) were taken. After 1 additional hour of light, the illumination was turned off. The end of night samples (EoN) were taken after 11 hours of darkness. This experiment was repeated 2 times for a total of 4 replicates per strain per condition.