RNA Polymerase II CoTC Terminator Elements in the Human Genome (CstF-64 CLIP-seq from the nucleoplasm)
CLIP-seq experiment using immunoprecipitation with CstF-64 antibody from the nucleoplasmic fraction, was employed to specifically target pre-mRNAs that are released from the DNA template before cleavage at the poly(A) site. This lead to the identification of genes that utilize the co-transcriptional cleavage mechanism of transciption termination.