RIP-seq analysis for RNAs associated with hTERT in nuclear speckles.
To investigate the function of p-hTERT in nuclear speckles, we isolated hTERT complex by immunoprecipitation with an anti-hTERT mAb from nuclear speckles purified biochemically and conducted RIP-seq analysis for RNAs associated with hTERT in nuclear speckles. Overall design: RNA Immunoprecipitation sequencing (RIP-seq) for hTERT in nuclear speckles biochemically purified  from HeLa cells. Two replicates were included.