Transcriptome analysis of CDAN1 deficiency in human erythroleukemic K562 cells.
Congenital dyserythropoietic anemia Type 1 (CDA1) is a rare macrocytic anemia caused by loss-of-function mutation of CDAN1. To investigate the functional role of CDAN1, we performed RNA-sequencing on human erythroleukemic K562 cells with CDAN1 shRNA knockdown using a doxycycline inducible system Overall design: Differential gene expression analysis comparing CDAN1 shRNA knockdown clones versus control samples including the respective uninduced clones together with both induced and uninduced cells expressing non-targeting (NT) shRNA