Transcriptomics analysis of neurogenesis by striatal astrocytes upon Rbpj-K deletion
We achieved local recombination of striatal astrocytes and silenced Notch signalling activity through Rbpj-K depletion. Recombined astrocytes and neural progenitors generated from the striaral glia were FAC sorted and their molecular profile investigated in a scRNAseq experiment performed 5 wpi. Overall design: Astrocytes and their neurogenic progeny isolated from striata of mice homozygous for the floxed Rbpj gene and subjected to local injection of AAV-Gfap-Cre