RNA-seq of two-week-old seedlings of wild type (Col-0), dph1 and dph2 mutants (in Col-0 background) growing on 0.5x MS media with 0.5% (w/v) sucrose under normal growth conditions (16 h light/8 h dark).
This is comparative transcriptomics by using the wild type, dph1 and dph2 mutants to see the change of dph1 and dph2 mutants at transcript levels compared with wild type. Seedlings of two-week-old plants grown in liquid MS media were harvested for total RNA isolation by RNeasy Plant Mini Kit (Qiagen). Libraries were prepared and sequenced using the Illumina platform by Novogene (Cambridge, UK). For RNA-seq data analysis, raw reads were first filtered by CutAdapt 2.1 to remove adaptors and low-quality reads. The filtered reads were mapped to Arabidopsis thaliana Col-0 reference genome (Tair 10). Read counts were determined by Qualimap2. Differentially expressed genes were identified using the R package DESeq2.