RNA-seq Analysis of the Effects of Lysophosphatidic Acid on Cutaneous Endothelial Cells
Mouse wound tissues were treated with or without lysophosphatidic acid (LPA), and endothelial cells were isolated at day 5 and day 7 after wounding by fluorescence-activated cell sorting (FACS) as CD31-positive, CD45-negative cells. Purified endothelial cells were subjected to bulk RNA-seq analysis to evaluate the effects of LPA treatment on endothelial gene expression during wound healing.