Gene expression analysis of hPSC derived macrophages (iMacs) co-cultured with hepatic cells
Purpose: to analyze change in gene expression of hPSC derived macrophages upon co-culture with hepatic cells including Huh7 and primary human hepatocytes Methods: SMART-seq2 amplified polyA RNA from sorted iMacs in the co-cultures.  Results: the transcriptome of iMacs changed after co-culture with Huh7 cells or pHHs, and changed after the HCV infection of the co-cultured Huh7 cells.  Conclusions: the gene expression profiles of co-cultured iMacs indicated that the culture conditions and the microenvironments could reprogrammed iMacs to adopt tissue-specific features and perform specifc functions. Overall design: Perform RNA-seq of control iMacs, and sorted iMacs from iMac-pHH co-cultures, and iMac-Huh7 co-cultures with or without HCV.