Transcriptome profile of pig cells with CBS gene knockout upon Aflatoxin B1 treatment
We used pig genome-wide CRISPR knockout library screening to identify the key host genes affecting Aflatoxin B1 (AFB1)-induced cell death in pig cell line IPI-2I. Through lenti-library transduction and AFB1 treatment screen, the survived cells were subjected to NGS to screen the gene candidates. The top candidate, cystathionine beta-synthase (CBS), shows great improvement to AFB1-induced cell death upon gene knockout. To explore the role of CBS in AFB1 cytotoxicity, we used RNA sequencing to analyze the globe gene expression changes in CBS-knockout and WT IPI-2I cells with and without AFB1 treatment.