Vitamin D Receptor Isoform VDRA Suppresses Hepatocellular Tumorigenesis by Restricting YAP Nuclear Localization
we established organoid cultures using residual tumor tissues obtained from HCC patients during surgical resection. Importantly, these cultured organoids consistently retained pathological features and markers closely resembling those of the corresponding primary tumors. Subsequently, we performed transcriptome sequencing Transcriptomic profiling demonstrated a strong correlation in gene expression between organoids and primary tumors , accompanied by minimal overall expression variability Collectively, these results indicate that the established organoid model faithfully preserves the genetic landscape of the primary tumors, thereby providing a robust and reliable platform for subsequent functional investigations.