Characterization of three light-responsive plant enhancers by Plant STARR-seq
We used Plant STARR-seq to dissect three plant enhancers derived from photosynthetic genes. The enhancers are derived from the Pisum sativum AB80 and rbcS-E9 genes and the Triticum aestivum Cab-1 gene. We measured the enhancer strength of nearly all possible single-nucleotide variants of these enhancers, and determined the enhancer strength of combinations of fragments derived from mutationally sensitive regions of these three plant enhancers.