Transcriptional characterization of  ABCC1 and ABCB1 KO and MZ1 treated WT HAP1 cell lines.
We used CRISPR/Cas9 to intoroduce frame-shifting mutations into the ABCC1 and ABCB1 genes and treated HAP1 WT cells with varying concentrations of the MZ1 PROTAC. To measure the transcriptional response to both genetic and chemical perturbances, we performed RNA-seq and quantified gene expression levels. Overall design: 6 cell lines, untreated or MZ1 treated in 1-3 technical replicates