Normal macrophage signaling and gene expression in Rosa26 Cas9-expressing mice
Cas9 expression in the Rosa26 “safe harbor” locus is widely used for gene manipulation and Crispr-based screening. Recently, experimental evidence suggested that macrophages isolated from Rosa26-Cas9 mice may have signaling differences compared to control mice in terms of TRIF signaling downstream of TLR3 and TLR4. As we frequently use the Rosa26-Cas9 mice made by Feng Zhang (Cas9-FZ, JAX stock No. 026179), arguably the strain with the widest distribution and utilization, we were motivated to test macrophage signaling in these mice under our conventional conditions. We used different macrophage polarization and signaling conditions combined with RNA sequencing and measurement of TLR signaling by immunoblotting. Our results suggest that the Cas9-FZ bears no obvious defects in any commonly used macrophage signaling pathway. We document the differences in our macrophage culture techniques compared to Raychowdhury et al., which may aid in how individual laboratories use Cas9-expressing macrophages, especially for focused or genome-wide screening. Overall design: RNA-seq profiling of wildtype C57BL/6N and Feng Zhang Cas9 mice differentiated with SCF1 and activated for 24 hours with IL4/IL13, LPS/IFNg, or a media control.