Directional endothelial communication by polarized extracellular vesicle release
EVs were isolated from primary human aortic endothelial cells (ECs) (+/- IL-1b activation), quantified, and analysed by miRNA transcriptomics and proteomics. Overall design: We performed miRNAHAEC EV-microRNA profiles were determined using HTG Molecular Diagnostic EdgeSeq technology (HTG Molecular Diagnostics, Inc, Tucson, AZ, USA).