B cell-intrinsic STAT3-mediated interferon suppression during murine gammaherpesvirus 68 infection revealed through in vivo competition model.[ccbr1030_Stat3_2021]
RNA-sequencing from sorted B cells of d16 infected mixed bone marrow chimeras. Non-GC, GC uninfected, and GC infected samples of STAT3 WT and STAT3 KO B cells from 3 independent mixed bone marrow chimera experiments. Overall design: 25,000 STAT3 WT or STAT3 KO non-germinal center and germinal center B cells, of which GC cells were both uninfected and infected with MHV68 (YFP+) were sorted, followed by RNA sequencing. Three independent experiments, infected GC samples had 2x samples per expeirment, for a total of 24 samples (3 of which failed QC).