Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [Cut & Run]
To investigate the role of ARID1A in the regulation of transcription factor binding and histone modifications in effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a KO mice. We then performed CUT&RUN against ARID1A, BATF, ETS, Tbet, H3K27ac, H3K27me3 in WT, Arid1a KO, or Tbx21 (Tbet) KO cells in vivo at d0, 48h in vitro activated, d5, and d8 post-LCMV Armstrong infection. Overall design: Transcription factor and histone modification profiling analysis by CUT&RUN for WT, Arid1a KO, and Tbx21 (Tbet) KO P14 CD8+ T cells at d0 (naive), 48h in vitro activated, d5 (total cells), d8 (TE, EEC, and MP cell subsets) following LCMV Armstrong infection.