Modulation of Amhr2 in mouse and human mesothelial cells
Amhr2 is as a specific biomarker for mesothelial cells. In this study, we investigated the impact of Amhr2 modulation on gene expression by overexpressing it in Met5a cells, a human mesothelial cell line. Additionally, we assessed the transcriptomic profile of tumor growth in Amhr2 knock-out mice. These experiments shed light on the role of Amhr2 in mesothelial cell function and its potential implications in tumor development Overall design: Bulk RNA sequencing of Met5a cells, overexpressing two variants of the Amhr2 receptor: Amhr2-V3(V3), which is an inactive receptor, and Amhr2-V1(V1), which is an active receptor. Bulk RNA sequencing of mouse KPCA tumors derived from wild-type mice (CON) (C57BL6J), Amhr2 heterozygous (Het) and Amhr2 knock-out mice (KO). This dataset compare the genes expression profiles between these two groups.