Identifying crossover locations in an Arabidopsis thaliana recq4 Col x recq4 Ler F2 population using Nanopore-seq and COmapper
Genomic DNA from 39 recq4 Col x Ler F2 individuals was extracted using the CTAB method. Equal amounts of DNA from these 39 plants were pooled and nine micrograms of gDNA from each pool was used to generate Nanopore sequencing libraries with the Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114). The libraries were sequenced independently using PromethION (BGI, Hong Kong).