Single-Cell Transcriptome Analysis of MAIT Cell Subsets from human normal intestine
Normal adjacent tissues from colorectal cancer patients were collected for MAIT cell sorting. Sorted MAIT cells from seven individual donors were pooled together. Single-cell RNA sequencing libraries were prepared using the Chromium 5' Single Cell Gene Expression Kit (10x Genomics), following the manufacturer's protocol. Sequencing was performed on a NextSeq 500 platform (Illumina).