White button mushroom intake modulates PMN-MDSCs, T, and NK cells' function at the single cell level in prostate cancer patients (validation cohort)
In prostate cancer patients following 3 months of white button mushroom consumption in freeze-dried tablet form, we observed the decline of circulating polymorphonuclear MDSCs (PMN-MDSCs), along with an increase of granzyme B positive CD8+ T cells and NK cells. Furthermore, single immune cell profiling of peripheral blood from WBM-treated patients revealed the transcriptional landscape of circulating immune cells. This methods showed a decrease in overall circulating PMN-MDSCs with suppression on the expression of STAT3/IRF1 and TGFß signaling. Subclusters of PMN-MDSCs showed transcriptional profiles associated with "response to fungus", "neutrophil chemotaxis", "leukocyte aggregation", and "regulation of inflammatory response", which are associated with enhanced immune-modulated anti-tumor activity. Overall design: Paired whole blood samples of four patients from timepoint of baseline vs. 3 months treatment were collected and processed based single immune cell profiling protocol. The leukocytes from whole blood were enriched according to 10X standard protocol