Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation
A key pathological feature of Parkinson's Disease (PD) is the progressive degeneration of dopaminergic neurons (DAns) in the substantia nigra pars compacta. Considering the major role of EN1 in the development and maintenance of these DAns and the implications from En1 mouse models, it is highly interesting to study the molecular and protective effect of EN1 also in a human cellular model. Therefore, we generated EN1 knock-out (ko) human induced pluripotent stem cell (hiPSCs) lines and analyzed these during neuronal differentiation. Although the EN1 ko didn't interfere with neuronal differentiation and generation of tyrosine hydroxylase positive (TH+) neurons per se, the neurons exhibited shorter neurites. Furthermore, mitochondrial respiration, as well as mitochondrial complex I abundance was significantly reduced in fully differentiated neurons. To understand the implications of an EN1 ko during differentiation, we performed a transcriptome analysis of human neuronal precursor cells (hNPCs) which unveiled alterations in cilia-associated pathways. Further analysis of ciliary morphology revealed an elongation of primary cilia in EN1-deficient hNPCs. Besides, also Wnt signaling pathways were severely affected. Upon stimulating hNPCs with Wnt which drastically increased EN1 expression in WT lines, the phenotypes concerning mitochondrial function and cilia were exacerbated in EN1 ko hNPCs. They failed to enhance the expression of the complex I subunits NDUFS1 and 3, and now displayed a reduced mitochondrial respiration. Furthermore, Wnt stimulation decreased ciliogenesis in EN1 ko hNPCs but increased ciliary length even further. This further highlights the relevance of primary cilia next to mitochondria for the functionality and correct maintenance of human DAns and provides new possibilities to establish neuroprotective therapies for PD. Overall design: To investigate the effect of EN1 during dopaminergic neuron differentiation on a human background, we generated EN1 knock-out human iPSCs using CRISPR/Cas9. In total, 3 EN1 knock-out lines and 5 wildtype lines were differentiated towards dopaminergic neurons. Gene expression profiling was performed at the stage of neuronal precursor cells. 3 replicates per cell line were analyzed. Neuronal precursor cells were stimulated with CHIR99021 to assess the effect of Wnt signaling and it's interaction with EN1 during dopaminergic neuron differentiation. Comparative analysis of RNA-seq data for wildtype and EN1 knock-out lines in unstimulated and Wnt stimulated conditions.