Genome-wide RNA-Seq analysis of mRNAs level in rat hippocampus at 24 hours after acute restrained stress
White 2-month-old male rats of the Wistar line (weight, 200-250 g) were used. The animals were divided into two groups: the "nonstressed" (NS) and "stressed" (ST). Each group contained 3 animals. All rats received intraperitoneal injection of vehicle (sterile distilled water in a volume of 1 ml/kg body weight). 30 min after injection, rats of the ST group were submitted to acute restraint stress for 1 h (immobilization of animals combined with bright light and acoustic exposure). Rats of the NS group were left undisturbed in their home cages throughout the stress procedure. Then, the animals were tested in an Elevated Plus-maze (within 5 min, at 23.5 h after injection). Rat was withdrawn from the experiment at 24 h after injection. mRNA fraction from total RNA of rat hippocampus was used for high-throughput RNA-sequencing (RNA-Seq). At least 10 million reads (1/50 nt) were generated.