Transcriptome analysis of ?dT cells with/without PERK knockdown
The global RNA expression was assayed in PERK knockdown and control ?dT cells exposed to a immunosuppressive mixed cell environment. Overall design: ?d T cells were activated and expanded from the PBMCs of healthy donors. Cells were transduced lentivirally with shControl or shPERK constructs. Cells were then incubated in an invitro TME system comprising of myeloid , endothelial and tumor cells for 48 hours and sorted out magnetically using CD3 microbeads. RNA was isolated and analyzed by RNAseq.