Effect of overexpress of circPPARG1 on gene expression during adipogenic differentiation of Intramuscular adipocytes
To investigate the function circPPARG1 in the regulation of adipogenic differentiation, we overexpressed circPPARG1 in Intramuscular adipocytes Overall design: First, Intramuscular adipocytes were planted in six-well plates in triplicate. When the cells reach 80% confluence, transduction was performed. Adenovirus were added to cells at the indicated MOI. Then, The culture medium was exchanged three hours later. Next, When transduction was performed for 48 hours, cells were treated with an inducing medium. Finally, Cells were collected for RNA sequencing after 48 hours of induction.