Raw sequence reads of Arabidopsis thaliana col-0 and At5g02860 mutant complemented with pABI3:At5g02860
PPR proteins are nuclear genome-encoded RNA-binding proteins that alter the expression of their target genes expressed from the chloroplast and mitochondrial genomes. At5g02860 encodes a previously uncharacterized PPR protein that is highly conserved in land plants. Homozygous knock-out mutation of At5g02860 is embryo lethal. Therefore, to characterize the function of At5g02860, we sequenced RNA from wild-type Arabidopsis and partially complemented At5g02860 knock-out mutants. Partial complementation of mutants was carried out by expressing At5g02860 from the seed-active ABI3 promoter in a homozygous At5g02860 knock-out mutant background. In the partially complemented lines, At5g02860 is expressed during embryogenesis and early germination, after which the promoter becomes switched off. Shoot tissue excluding cotyledons was harvested from 20-day-old seedlings. Total RNA was extracted, after which RNAseq libraries were prepared using the TruSeq stranded total RNA kit with Ribo-Zero (Illumina). Small RNA libraries were also prepared from the same RNA preps using the NEBNex Multiplex Small RNA Library Prep Set for Illumina (NEB).