Bulk RNA sequencing of cardiomyocytes differentiated in monolayer (2D) from hiPSC-derived cell lines with tetracycline-inducible knockdown of CTCF and GATA4
This dataset was generated to study the role of the DNA-binding factors CTCF and GATA4 in cardiac specification. Three WTC11-derived hiPSC lines were used, each carrying a stably integrated shRNA construct. Two constructs specifically target CTCF and GATA4, while the third expresses a scrambled, non-targeting shRNA as control. All shRNA systems are controlled by a tetracycline-inducible promoter, enabling conditional knockdown.  Each of the three lines was differentiated into a 2D monolayer of cardiomyocytes, both with and without tetracycline treatment, providing an isogenic control for each knockdown. For every condition, two independent differentiations were performed to ensure reproducibility.