Gene expression profiles of S. cerevisiae HAC1i and hda3D cells
The unfolded protein response (UPR) is a cytoprotective gene induction response that is induced upon dysfunction of the endoplasmic reticulum (ER), namely ER stress. In yeast Saccharomyces cerevisiae cells, the UPR is triggered by the ER-located transmembrane endoribonuclease Ire1 that, in response to ER stress, splices the HAC1 mRNA to yield the HAC1i mRNA, which is translated into an active transcription factor being responsible for the UPR. Therefore, the UPR is constitutively induced even without ER stress stimulu when cells carry a mutation to have the spliced HAC1i sequence on the HAC1 locus. To find the genome-wide gene expression change by the UPR and to understand how histone deacetylation by the HDA complex affect to it, here we preformed mRNA-seq analysis of S. cerevisiae cells with the following mutations; ire1D, ire1DHAC1i, ire1Dhda3D, and ire1Dhda3DHAC1i.