Sequencing of HIV splicing products
The hnRNP A2B1-KD data was derived from two experiments, each with three separate transfections including a Control- and a hnRNP A2B1 siRNA-transfected sample, while the hnRNP A3-KD data was derived from one experiment with two paired Control vs. hnRNP A3 siRNA transfections (I originally did three transfection for this experiment as well, but one sample had low RNA and was excluded as an outlier). All of this was done in HeLa cells, and samples were harvested at 24 hours post-siRNA transfection.