PacBio sequencing of ash and rowan leaves
We subjected DNA extracted from unwashed and washed leaflets of healthy and diseased European ash to PacBio sequencing of the fungal ITS1-5.8S-ITS2 rDNA region in order to gain insight of the European ash (Fraxinus excelsior L.) leaf niche partitioning and understand the fungal community receptiveness to the invasion of H. fraxineus. Leaflets from co-inhabiting rowan trees (Sorbus aucuparia) served as a reference.