Red vs. far-red light response in Cuscuta campestris
Cuscuta campestris shoot tips were attached to wooden sticks by loosely winding them one or two turns. They were then exposed to controlled light regimes: 16 hours of white light supplemented with either red or far-red light, followed by 2 hours of monochromatic red or far-red light, and 6 hours of darkness. Shoots were harvested during the monochromatic light phase after 1 and 3 days for mRNA analysis, and on day 3 for DNA methylation profiling. Small RNA libraries were generated from the same day 3 RNA pools used for mRNA analysis. For each sample, coiled regions from different shoots were trimmed under green light, pooled in triplicate, and frozen in liquid nitrogen. Untreated shoots collected prior to light exposure served as controls.