Inflammatory milieu by crosstalk between podocytes and proximal tubular cells in type 2 diabetic kidney disease
Due to the limited availability of therapeutic agents for type 2 diabetic kidney disease (DKD), there is a need for further knowledge derived from experimental models and innovative techniques. In addressing this issue, single-cell RNA sequencing (scRNA-seq) has been exclusively applied to a genetically modified DKD model, but not an induced model representing type 2 DKD. Herein, we analyzed scRNA-seq and other experiments from an induced type 2 DKD model, and validated the results in human-derived biospecimens. The model was induced by combining a high-fat diet with streptozotocin to simulate induced type 2 DKD. scRNA-seq, histological, and flow cytometric analyses were conducted, and their results were compared with control mice. The findings were then applied to human kidneys exhibiting diabetic glomerulosclerosis associated with type 2 diabetes. Overall design: single-cell RNAdata for induced type 2 DKD with HFD/STZ