Examination of Genome-Scale CRISPR Screening in mouse stomach organoids.
Epithelial organoid cultures accurately recapitulate many features of in vivo epithelial tissues, providing an excellent ex vivo platform for interrogation of key regulatory mechanisms. We report a Genome-Scale CRISPR Knock-Out (GeCKO) screening assay using mouse gastric epithelial organoids to identify novel modulators of Wnt-driven stem cell-dependent epithelial regeneration in the gastric musosa.