Transcriptomic analysis of enamel organ in a mouse model of Loeys-Dietz Syndrome Type II harboring a G357W mutation in the Tgfbr2 gene
Loeys Dietz Syndrome (LDS) is a connective tissue disorder caused by mutations along the TGF-beta signaling pathway and characterized by severe aortic aneurysm and craniofacial anomalies. Patients with LDS Type II (LDS2), caused by mutations in the TGFBR2 gene also exhibit enamel defects. A mouse model for LDS2, harboring a G357W mutation in the Tgfbr2 gene, recapitulates the cardiovascular, craniofacial and dental phenotype of the disease. This transcriptomic analysis aimed at identifying genes that are differentially regulated in the enamel organ of Tgfbr2-G357W/+ mice at postnatal day 11. Total RNA was extracted from enamel organs from five Tgfbr2-G357W/+ mice (LDSR2, KI) and eight Tgfbr2-+/+ wild-type littermates (WT). This dataset indicates that the mutation in Tgfbr2 in the enamel organ minimally affects gene expression and suggests that the enamel phenotype in LDS2 is not dependent on canonical TGF-beta signaling. Overall design: Total RNA from enamel organs (from mandibular incisors) micro-dissected from five Tgfbr2-G357W/+ mice (LDSR2, Het) and eight Tgfbr2-+/+ wild-type littermates (WT), at postnatal day 11, was collected for bulk RNA-seq analysis.