Canis lupus familiaris Transcriptome or Gene expression
Respiratory viral-bacterial co-infections cause severe disease across species, yet the molecular mechanisms underlying enhanced pathogenesis remain poorly understood. This project provides RNA-sequencing data from DH82 alpha canine macrophage-like cells co-infected with H3N8 equine influenza A virus (IAV) strains A/equine/Sussex/1989 and A/equine/Newmarket/5/2003 for 24 h and then with Streptococcus equi subspecies zooepidemicus (SEZ) for 6 h, or infected with active or heat-inactivated SEZ alone. RNA-seq analysis revealed that bacterial infection was the primary driver of transcriptional changes during co-infection, with minimal differences in differentially expressed genes between SEZ alone versus co-infection. The dataset characterises host transcriptional responses to single and co-infection with these important equine respiratory pathogens, providing insights into the molecular basis of viral-bacterial co-infection pathogenesis.