Sequencing of the microbial composition of AD mice after fecal transplantation
Raw paired-end sequencing data in FASTQ format were preprocessed using Cutadapt to identify and remove adapter sequences. Following adapter trimming, low-quality sequences were filtered out. Denoising, merging of paired-end reads, and chimera removal were performed using the DADA2 plugin in QIIME 2 (v2024.2, Northern Arizona University, USA) with default parameters. The final outputs included representative sequences for each Amplicon Sequence Variant (ASV) and an ASV abundance table.