MDA-MB-231 EglN1-Flag IP-MS
This study aimed to investigate the role of EglN1 under normoxic and hypoxic conditions in MDA-MB-231 cells. MDA-MB-231 cells were transfected to overexpress EglN1-Flag, and then treated with normoxic and hypoxic conditions respectively. Immunoprecipitation (IP) was performed using an anti-Flag antibody to isolate EglN1-Flag protein complexes from the cell lysates. The isolated complexes were then analyzed by mass spectrometry (MS) to identify the interacting proteins of EglN1. The raw data of IP-MS presented here contain the mass spectrometry readings and associated metadata from these experiments, which provide a comprehensive resource for understanding the molecular mechanisms underlying EglN1 function in breast cancer cells under different oxygen conditions.