16s rRNA sequencing of gut microbiota in mice
The current study showed that early-life supplementation of probiotics improved dysbiosis of gut microbiota. More importantly, we identified Faecalibaculum rodentium which was depleted in the gut microbiota of mice fed with HFD but restored by early-life supplementation of probiotics. Intragastric administration of F. rodentium CCAM966 benefited intestinal homeostasis, reduced body weight gain and BMI, and rescued the glucose, lipid and bone metabolic disorders induced by HFD in adult mice. Our findings indicate that early-life supplementation of probiotics protects the mice from the development of MetS-related dysfunctions in adulthood, likely by enriching F. rodentium in gut microbiota. Supplementation of F. rodentium benefits the prevention of glucose/lipid/bone metabolic disorders under HFD challenge, representing a promising probiotic strain for the better management of MetS.