Total RNA transcriptomic profiling of mouse thoracic and abdominal perivascular adipose tissue of mice with an adipocyte-specific deletion of PRDM16 and flox control mice [snRNA-seq]
Thoracic and abdominal perivascular adipose tissue (tPVAt and aPVAT) is in direct contact with the outer layer of the aorta. While tPVAT exhibits a thermogenic phenotype, aPVAT is mostly comprised of white adipocytes with a few scattered thermogenic adipocytes. To investigate the relevance of this change in adipocyte identity along the aorta, we used mice with an adipocyte-specific deletion of Prdm16 resulting in loss of thermogenic and gain of white adipocyte-like features and compare gene expression signature in these mice. Overall design: Total mRNA RNAseq was performed on total RNA extracted  from PRDM16flox (controls) and PRDM16flox, AdipoQCre+ mice.