Transcriptomics of BMDM treated with PTEN in the presence or absence of STAT1 inhibitors
PTEN binds to and regulates polarization of TAMs to enhance antitumor immunity and suppress tumor growth,We hope to investigate whether STAT1 mediates the polarization effect of PTEN on TAMs as a downstream factor Overall design: Bone-marrow (BM) cells were harvested from femurs and tibia of C57BL/6J mice. Differentiation was induced by recombinant mouse M-CSF in DMEM medium containing 1% penicillin/streptomycin and 10% FBS for 7 days to obtain the bone marrow-derived macrophages (BMDMs).To induce an M2-like phenotype, we treated BMDMs with IL-4 and IL-13 for 48 h.M2 BMDMs were treated with and without 100 ng/mL PTEN for 24 h in the presence or absence of STAT1 inhibitors (Fludarabine), followed by RNA-seq analysis.