Transcriptomic profiling of peroxisome-deficient, myelin debris-laden macrophages
Study objective was to investigate the molecular underpinnings of how Pex5 and peroxisome integrity facilitated the demyelination response in myeloid cells. We investigated the transcriptomic changes related to myelin debris uptake in the context of disrupted peroxisome integrity using Pex5 deficient bone marrow derived macrophages. Overall design: Cultured bone marrow derived macrophages (BMDM) were derived from control and Pex5KO mice. BMDM were incubated with 0.5 mg/mL of crude myelin debris for 24 hours and 72 hours duration. Resting BMDM that did not receive myelin debris were used as baseline controls. After incubation with myelin debris for the indicated durations, total RNA was extracted from BMDM and submitted to Novogene for high-throughput sequencing.