Establishment and Differentiation of 3D Equine Intestinal Organoids and Single-Nucleus Transcriptome Sequencing
Intestinal organoids are three-dimensional in vitro culture models that can simulate the structure and function of in vivo intestinal epithelium, providing an important tool for studying intestinal physiology, pathology, and drug development. In this study, 3D equine intestinal organoids (EIO) were successfully established using intestinal tissue samples, and induced to differentiate into mature EIO with typical intestinal epithelial cell characteristics. To systematically analyze the transcriptional heterogeneity and molecular characteristics of different cell subsets in equine intestinal organoids (including undifferentiated and differentiated states), single-nucleus transcriptome sequencing (snRNA-seq) was performed on the established organoids. This dataset will help researchers deeply understand the transcriptional regulation mechanism during the establishment and differentiation of EIO, and lay a foundation for the application of EIO in basic research and clinical translation.