Analysis of molecular mechanism of HPV18 carcinogenesis by transcriptome sequencing
In order to study the molecular mechanism of HPV18 carcinogenesis, we used conditional reprogramming technique to culture normal cervical epithelial cells, cervical intraepithelial Neoplasia HPV18 cells and patient-derived cervical cancer cell lines for 24 h and stimulated with estradiol for 24 h,and collected total RNA samples for mRNA sequencing (RNA-SEQ). Then, Gene expression profiling was performed using RNA-seq data from six groups of cells. Overall design: Comparative gene expression profile analysis of RNA-seq data in normal cervical epithelial cells group, normal cervical epithelial cells - estrogen stimulated group, cervical intraepithelial Neoplasia HPV18 cells group, cervical intraepithelial Neoplasia HPV18 cells - estrogen stimulated group, patient-derived cervical cancer cell group, patient-derived cervical cancer cell - estrogen stimulated group.