RNAseq of effects of high glucose environment on differentiated primary osteoblasts
The aim of this study was to investigate the global transcriptional changes induced by high glucose environment in primary osteoblasts in vitro. We isolated bone marrow stromal cells from 4-week-old Sprague-Dawley rats, differentiated them into osteoblasts and exposed them to a short- (one or three days, abbr. HG1d and HG3d) or a long-term (ten days, HG10d) high glucose environment, after which global transcriptional changes were studied using mRNA sequencing.  Overall, the cells were differentiated for 10 days and high glucose treatments were started on days 9, 7, and 0 of differentiation.