Manihot esculenta subsp. esculenta Raw sequence reads
Cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) infection had been identified as a major pernicious disease in cassava plantations and is widespread in Southeast Asia, especially Thailand, which is one of the main worldwide cassava supplier country. Potential options to restrict the expansion of SLCMV were included to generate a new resistant cultivar, restricting virus colonization by using a phytosanitary measurement, identifying and studying of the tolerant cultivars under an insight suspect on the gene regulation from the perspective of regulatory transcribed genes and mechanism within the plant innate immunity and adaptation mechanisms. However, the SLCMV infection procedure and the host plant defense interaction were less understood, especially regarding the knowledge of virus-derived gene regulation. Here, we defined the transcriptome profiles in a tolerant cultivar named Kasetsart 50 (KU 50) and a susceptible cultivar called Rayong 11 (R 11) at 21-, 32- and 67-days post inoculation (dpi) by using the Next Generation Sequencing (NGS) tool to determine the transcripts/genes during the SLCMV infection, we aimed to study the conflicted of expressed genes based on a paired comparison of the SLCMV-infected cassava cultivars in between suspected at a tolerant and a susceptible cultivar which characterized by their phenotypic variations. The transcriptome results showed that 55,699 genes were discovered in this research and differential expressed genes (DEGs) were also explored which then reinforced by using the RT-qPCR technique for gene validation, the differential genes that expressed in each among of infection stages were determined genes as responded genes to against and regulate in the viral infection during those phases. As an assumption from our transcriptomic profile comparison between a tolerance and susceptible cultivars, by highlight the role of controlling gene regulations and its association with plant phenotypic variation, as illustrated by the current case study of a viral pathogen, this research was revealed an identified genes were involved in epigenetic modification, transcriptional and transcription factor activities, plant defense and oxidative activities, and hormones and metabolic mediated pathways, particularly in the tolerant cultivar.