Mus musculus Transcriptome or Gene expression
Rapid scar-free wound repair is a key goal in regenerative medicine. In this study, a dynamically Schiff base crosslinked hydrogel with phase-adaptive functions is developed to enhance rapid reepithelization while minimizing scar formation in chronic infected wounds. The hydrogel effectively eliminates multidrug-resistant bacterial biofilms during the infection stage through the antimicrobial activity of polylysine, which dissociates from the gel matrix in the infection environment. It also interrupts the oxidative stress and inflammatory cycles at the wound site through the release of ceria nanozyme, creating a pro-regenerative environment for tissue repair. Fibroblast growth factor and cJun siRNA-loaded microcapsules gradually release their contents, promoting neoangiogenesis and cell proliferation, while inhibiting cJun overexpression to prevent fibrotic scar formation. Importantly, the hydrogel facilitates skin regeneration without noticeable scars in both infected male mouse and rabbit ear wound models. This approach offers a promising regenerative strategy, focusing on immunomodulation and fibroblast subtype regulation for scarless wound healing.