Single-cell transcriptional profiling of wildtype Rosa26(YFP) (Y) and Kras(G12D) p53(-/-) Rosa26-YFP (KPY) lung AT2 epithelial cells, AT2 organoids, and co-cultured mesenchymal cells with temporal resolution
The aim of this study was to examine the transcriptional dynamics of AT2 progenitor cells and their surrounding mesenchyme in lung adenocarcinoma across model systems, using temporal and single-cell resolution. Overall design: Examination of the progression of wildtype Rosa26(YFP) and Kras(G12D) p53(-/-) Rosa26(YFP) AT2 cells in organoids, their co-cultured wildtype mesenchymal cells, and GEMMs at various time points after induction using Ad-Cre.