Plasmodium falciparum whole genome sequencing using ONT
Whole genome sequencing is vital for malaria surveillance. Traditional short-read approaches have difficulty analyzing the complex Plasmodium falciparum genome, especially repetitive and structural regions. Long-read sequencing, such as Oxford Nanopore, overcomes these limitations and is well-suited to low-resource environments. This work presents an optimized Nanopore-based protocol for sequencing P. falciparum from dried blood spots and whole blood.