Characterisation of TET function during indirect flight muscle development
Enzymes of the Ten Eleven Translocation (TET) family play a key role in the regulation of gene expression in many species by oxidizing 5-methylcytosine (5mC), a prominent epigenetic mark, into 5-hydroxymethylcytosine (5hmC). Yet, TET proteins also have non-canonical modes of action beyond 5mC oxidation, notably in Drosophila, whose genomes is devoid of 5mC. Here, we assessed TET function during the development of the adult indirect flight muscles which are derived from a population of progenitors located on the larval wing imaginal discs. Overall design: All experiments were performed with biological replicates using dissected third instar larval wing discs or dissected pupal indirect flight muscles. w1118 (wt) or TET-GFP knock-in flies were used as control lines. Drosophila homozygous for Tet null or Tet catalytic dead alleles were used as test lines.