16S rRNA sequencing of Villin-Cre-ERT2 Ap1m2flox/flox mice
To investigate the effect of the intestinal epithelial barrier maintained by AP-1B on the gut microbiota, this experiment compared the ileal microbiota composition of intestinal epithelial cell-specific Ap1m2-deficient mice with that of control mice. Ileal contents were collected from Villin-Cre-ERT2 Ap1m2flox/flox mice on days 15 after the first tamoxifen administration and control mice.  After DNA extraction from ileal contents, DNA samples were amplified by PCR with primers for 16S rRNA V3-V4 regions and were attached to dual indices. After purification, the libraries were diluted and sequenced on the MiSeq system (Illumina) with 300 bp paired-end reads.