In vivo studies of DNA-binding protiens in Methanosarcina acetivorans
This project investigated the in vivo phenotypes of DNA-binding protein deletion strains in the model methanogen Methanosarcina acetivorans. Deletion strains were made of all known proteins with a potential role in nucleoid architecture. This included the sole archaeal histone encoded by this organism, HmaA, as well as both copies of archaea-specific nucleoid-associated protein, MC1 (copies are designated MC1a and MC1b, respectively). Data included in this project are whole genome-sequencing results of deletion strains, as well as RNA-sequencing data for each single mutant strain. Data from this project can help elucidate the cellular roles of histones and archaea specific nucleoid-associated proteins across the archaeal domain; they also provide new insight into the cell biology of this environmentally-relevant microbe.