16S rRNA sequencing of lung microbiota in patients with non-cystic fibrosis bronchiectasis
Bronchoalveolar lavage fluid (BALF) samples from 23 patients (8 PA-colonized, 15 non-colonized) underwent 16S rRNA gene sequencing. Microbial diversity and taxonomic composition were analyzed. An XGBoost model with SHapley Additive exPlanations (SHAP) analysis was constructed to assess exacerbation risk, focusing on microbial and inflammatory markers.