Study on the Nitrogen Removal Performance and Microbial Response Mechanism of Multi-Factor Regulated Aerobic Denitrification Reactor Treating Aquaculture Wastewater
This study conducted high-throughput sequencing of 16S rRNA genes (V3-V4 region) on biofilm samples collected from different functional zones (A to F) and operational phases (varying temperature, hydraulic retention time, and influent nitrate loading) of an aerobic denitrification reactor. The data systematically analyzed microbial community diversity, composition, and their responses to environmental factors. The sequencing results revealed that temperature was the dominant factor driving changes in community structure, with Pseudomonadota as the predominant phylum (average relative abundance >75%). High nitrate loading enriched denitrifying functional genera (such as Simplicispira and Pseudomonas), but their functional expression depended on the synergistic effects of HRT and temperature. Under low-temperature conditions, community richness decreased, but the relative abundance of certain cold-adapted genera (e.g., Simplicispira) increased. This dataset provides a crucial foundation for understanding the succession patterns and functional mechanisms of denitrifying microorganisms under the coupling of multiple environmental factors.