miCLIP, RNA-Seq in HEK293E cells treated with Torin1
m6A RNA methylation and transcriptome profiling in were performed HEK293E cells following treatment with Torin1 Overall design: miCLIP and RNAseq was performed on twice poly(A)-purified mRNA from HEK293E cells that were serum starved overnight and treated with insulin (200 nM, 30h) with or without pretreatment of Torin1 (250 nM).  All experiments were performed in biological triplicate.