Effect of circadian disruption on mouse gut microbiota
The goal is to assess the impact of circadian rhythm disruption on on gut microbiota profile. Sixteen C57BL/6J mice (8 male/ 8 female) from Jackson Labs (The Jackson Laboratory, Bar Harbor, ME, USA), aged 33-weeks, were randomly treated to one of two experimental groups (n=4 per group per sex) and housed individually with a running-wheel. Animals were placed on a 12-hour light/12-hour dark (LD) cycle for 2-weeks before the beginning of this experiment. Half of the mice were maintained under the standard (12:12 - LD) photoperiod (control group) and half were exposed to constant light (24:0 - LL) (experimental group) for 10 consecutive days to challenge the circadian system.