Robust reprogramming of glia into neurons by inhibition of Notch signaling and NFI factors in adult mammalian retina.
scRNA-Seq was performed on  adult control and Rbpj-deleted mouse retinas to profile changes in gene expression. Overall design: scRNA-seq were prepared on dissociated retinal cells using the 10X Genomics Chromium Single Cell 3' Reagents Kit v3.1 (10X Genomics, Pleasanton, CA). Libraries were constructed following the manufacturer's instructions. Sequencing data were processed through the Cell Ranger 7.0.1 pipeline (10X Genomics) using default parameters.