The role of RUNX1 chromatin interaction in follicle functions during pre- and peri-ovulation (ChIP-seq)
RUNX1 is induced by the LH surge in the ovary and plays an important role in granulosa cells in the peri-ovulatory window through the induction of key gene expression. The ablation of the canonical heterodimer partner of RUNX1 (CBFß) leads to ovulatory failure and subfertility in female mice. A possible interaction between RUNX1 and PGR on the chromatin level has also been indicated. However, the properties of RUNX1 chromatin binding in granulosa cells in response to the LH surge remains unknown. This study aims to characterise the RUNX1 cistrome in pre- and peri-ovulatory granulosa cells and explore the potential interaction between RUNX1 and the key ovulatory factor PGR. Overall design: 2 replicates of RUNX1 ChIP-seq were performed from granulosa cells pre- and post-hCG stimulation, with pooled input control for each time point