Hepatic circadian gene expression in WT and liver specific Egr-1 knockout mice aged 2month, 6month and 12month
The circadian rhythms of older individuals are normally shifted, and the metabolic functions of these individuals are degraded. How the link between metabolism and circadian rhythms is decoupled in peripheral metabolic organs with age increased is still largely unknown. Here, we analyze hepatic transcriptome in the liver at zeitgeber time (ZT) 1, 5, 9, 13, 17, 21 from C57BL/6J aged 2month, 6month and 12month. Transcriptional changes suggest that the peripheral circadian system is shifted and that the rhythmic lipid metabolism is disrupted in the liver with aging. Furthermore, we figure out liver EGR-1 might integrate the central and peripheral rhythms and regulate metabolic homeostasis in the liver. Another hepatic transcriptome of WT and Egr-1-KO liver samples at EGR-1 highest(H) and lowest(L) zeitgeber time in 2month, 6month and 12month were analyzed. The results indicated that the rhythmic coupling of EGR-1 and CIDEA regulates age-related metabolic dysfunction in the mouse liver. Overall design: Liver gene expression in WT and liver specific Egr-1 knockout mice aged 2month, 6month and 12month