In-Flight Human Skeletal Muscle Tissue Chip Autonomous Payload: Validation of Fiber Type and Metabolic Changes Due to Space Flight
Our muscle tissue chip platform provides a novel approach to studying the cell autonomous effects of microgravity on muscle cell biology Overall design: CD56 myoblasts-derived from young active and old sedentary muscle biopsies, differentiated into functional myobundles, that exhibited classic skeletal muscle bio-functions, were subjected to microgravity. We report on the differences in gene expression  profiling between the flight young- vs old-derived myobundles and ground young- vs old-derived myobundles. A minimum of three biological replicates was used for RNA seq.