Gut microbial 16S rRNA sequencing on BTBR mice
Accumulating evidence links the gut microbiota to ASD symptoms via the gut brain axis, yet specific microbial markers and their therapeutic mechanisms remain unclear. Based on our previous identification of a candidate microbial ASD marker, we performed bacterial intervention in the BTBR ASD mouse model. To evaluate microbial changes following intervention, we performed 16S rRNA sequencing on colon biopsies, cecal contents, and stool samples.