Genomic characterization and coordinate-anchored substrate benchmarking of the Qiantang River Megalobrama sample SJF
This project provides high-depth Illumina short-read sequencing data (approximately 100.14 Gb) for a wild female Megalobrama individual (sample SJF) collected from the Qiantang River, China. Given the complex taxonomy and nomenclature within the Megalobrama genus, this dataset was generated to establish a robust, read-backed sequence substrate and to evaluate the genomic response to projection across alternative chromosome-level M. amblycephala coordinate systems.The sequencing was performed on the Illumina NovaSeq X Plus platform with a 350-bp insert size library, yielding 670,751,604 high-quality paired-end reads. Our study utilizes these data to resolve the "projection-interpretation gap" in non-model fish genomics by explicitly partitioning a stable callable core from a reference-sensitive structural shell. This dataset serves as a foundational resource for downstream evolutionary analysis, population genetics, and germplasm conservation of the Qiantang River Megalobrama lineage, providing a reproducible methodological template for interpreting fragmented drafts in a coordinate-anchored framework.