Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells
Objective The relatively unique IPEC-J2 cell line is used as an in vitro small intestine model for swine, but it is also used as a model for the human intestine. Within a series of experiments, including electric cell-substrate impedance sensing technology, we showed that mRNA gene expression profiles and related pathways can depend on the growth phase of IPEC-J2 cells. Our treatment and sampling experiments welcome scientists to reproduce or modify our protocols and to put their gene expression data in the context of the respective growth phase of the cells. Results Three timepoints are presented: 1 hour after medium change (= 6 hours after seeding of cells, TP1), the timepoint of the first derivative maximum of the cell growth curve (TP2) and one point at the beginning of the plateau phase (TP3). Significantly outstanding at TP1 compared to TP2 was upregulated PLEKHN1, further FOSB and DEGS2 were significantly downregulated at TP2 compared to TP3. Any provided data can be used to improve next-generation experiments with IPEC-J2 cells.