CREB and H3K4 CUT&RUN seq of mouse fetal testis
CUT&RUN analyses to examine CREB genomic binding sites in E13.5, E16.5, and E18.5 XY gonads, with H3K4me3 serving as a positive control indicative of active transcriptional regions. Overall design: Male gonads were dissected and dissociated into individual cells by pipetting. The resulting cell suspension was filtered using a 70 µm filter, and 100,000 gonadal cells were used for CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assays following an alternative protocol from Protocol.io (dx.doi.org/10.17504/protocols.io.zcpf2vn).