Mineralocorticoid receptor dependent gene expression in cardiac endothelial cells
Mice with endothelial cell-specific deletion of the MR were generated as described using a tamoxifen-inducible Cre/loxP system. Mice expressing Cre under the control of the cadherin 5 promoter (Cdh5Cre-ERT2) were crossed with mice carrying a floxed MR allele (MRflox). Cre-negative (MRwildtype) mice were used as a control. MRCdh5Cre and MRflox mice received aldosterone from subcutaneously implanted osmotic minipumps for 14 days.