RNA-seq of BV2 cultured microglia following high glucose and CD200Fc treatments
We observe dyseregulated CD200-CD200R signaling in early diabetes that correlates with microglia-mediated neuroretinopathy and found that CD200 fusion protein (CD200Fc), an agonist of CD200R, attenuated high glucose-induced inflammation in cultured microglia. Therefore, we performed RNA-Sequencing on cultured BV2 microglia following exposure to normal or high glucose media (NG, HG) with or without CD200Fc supplementation (50ng/mL) to investigate broader biologic implications. Overall design: We performed comparative gene expression profiling analysis of RNA-Seq data from BV2 microglia RNA samples following treatment with normal or high glucose media (NG, HG) that is also supplemented with or without CD200Fc (50ng/mL)