ERG-driven gene expression in basal and luminal cells of mouse prostate organoids
ETS transcription factor ERG is aberrantly activated in ~50% of human prostate cancers. We found that ERG shows a preference of basal over luminal context to drive prostate cancer. However, the molecular basis of the cell context dependency remains elusive. Here we use ERG+/+;Pten-/- (EP) mouse prostate organoids with dual basal/luminal lineage reporters to profile ERG-driven gene expression programs from sorted basal vs luminal cells. Overall design: ERG+/+;Pten-/- mouse prostate organoids treated with either NT sgRNA (N) or sgERG (E) were flow sorted to isolate basal (B) and luminal (L) cells a month post CRISPR. Sorted population were subject to RNA-seq profiling.