RNA-seq of TM3 Leydig and TM4 Sertoli cells under MEHP exposure
This study was designed to investigate the toxicological effects of MEHP on TM3 Leydig and TM4 Sertoli cells. Overall design: For experiments, TM3 Leydig and TM4 Sertoli cells were seeded at 1.5 × 105 cells/ml in 2 ml/well in 6-well plates. After 24 hours, cells were exposed to 100 uM mono-(2-ethylhexyl) ester (MEHP; AccuStandard, ALR-138N), a major metabolite of DEHP in vivo, for another 72 hours. MEHP was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, D2438), with 0.1% DMSO treatment used as a control.