CircRNA MATCR encoded protein as a novel endogenous regulator in major depressive disorder by binding to heat shock protein 90 and inhibiting glucocorticoid receptor nucleus translocation
Short hairpin RNA (shRNA) based on si-MATCR2 sequence was assembled to lentiviral delivery vectors. Empty and shRNA vectors were separately transfected into SH-SY5Y cells to create control or stable MATCR-knockdown cell lines. High-throughput RNA sequencing of these two cell lines were performed and analyzed.