Transcriptomics of Gcn5-knocked down larvae and epigenomics of Drosophila melanogaster larvae fed on a diet rich in oleic acid, valine and acetate
[Whole-body RNA-seq 1] Control larvae were fed with a yeast strain (BY4741) and a medium containing Tween 80. Experimental larvae were fed with BY4741 and a medium containing Tween80, oleic acid, valine and acetate. RNA was extracted from whole bodies of these female wandering 3rd larvae sequenced in single-end mode.  [Whole-body RNA-seq 2] DaGS > Gcn5 RNAi larvae were fed with a laboratory standard food with ethanol (Gcn5 KD-) or 2 uM RU486 (Gcn5KD+). RNA was extracted from whole bodies of these male or female wandering 3rd larvae, respectively. [CUT&RUN sequencing] Larvae were fed on the control diet, the OVA diet (rich in oleic acid, valine, and acetate) or the OV diet (rich in oleic acid and valine). These 3 groups of wandering 3rd male larvae were dissected and incubated with an anti-H3K9ac or IgG antibody, respectively. The DNA was cleaved, isolated, and sequenced in paired-end mode.