Effect of overexpression of ESRP1 on gene expression of SGC7901 gastric cancer cells
RNA-binding proteins and their mediated alternative splicing play important roles in tumor cell invasion and migration. Here, we report that ESRP1 is a key regulator of gastric cancer cell metastasis. Overexpression of ESRP1 inhibits the invasion and migration of gastric cancer cells, in vivo and in vitro. Furthermore, we found that ESRP1 causes a wide range of alternative splicing events, and ESRP1-mediated CLSTN1 exon skipping may be a key mechanism for its inhibition of gastric cancer cell invasion and metastasis. Taken together, our data provide a molecular framework for the role of ESRP1 in gastric cancer development. Overall design: In order to study the role and mechanism of ESRP1 in the invasion and migration of gastric cancer cells, we established the SGC7901 gastric cancer cell line with stable overexpression of ESRP1. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells.