Molecular mechanism of EP300 in AC16 cell line
Functional studies found that knockdown of EP300 ameliorated the drug-induced cellular senescence phenotype. Here, we apply transcriptomics to explore the underlying molecular mechanisms. Overall design: Cardiomyocyte AC16 cells were cultured in DMEM/F12 and DMEM (high glucose), respectively. DMEM supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 U/mL penicillin, and 100 µg/ml streptomycin. Medium without FBS was replaced before transfection. Negative control siRNA and EP300 siRNA were transfected into AC16 cells at ~70% confluence using jetPRIME® (Polyplus-transfection, NY, USA). After 12h of transfection, AC16 cells were treated with H2O2 (200mM) for 1 h, then fresh complete medium was replaced. After 48 h, RNA  extracted according to the manufacturer's instructions using Trizol reagent. RNA libraries were prepared for sequencing according to manufacturer's instructions of Beijing Genomics Institution (BGI)