Genome-wide CRISPR/Cas9 screen of CEACAM3-mediated phagocytosis by human HL60 cells
Raw sequencing data of sgRNA sequence amplicons resulting from human HL60 cells transduced with a lentiviral genome-wide sgRNA library. Cells were selected by flow cytometry before and after phagocytosis of fluorescently-labeled CEACAM3-binding bacteria. Next, sgRNAs encoded by the selected HL60 cells were amplified by PCR and the sgRNA sequences determined via Illumina sequencing. Please see Kuiper et al. for detailed description.