Tryptophan Metabolite Norharman Secreted by Cultivated Lactobacillus Attenuates Acute Pancreatitis as an Antagonist of Histone Deacetylases
Rftn1 knockdown of differentially expressed genes in RAW264.7 cells was detected by siRftn1 and shRftn1. RAW264.7 cells were treated with norharman or medium for 24 hours. Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions and genomic DNA was removed from the preparation using DNase I. The library was prepared and sequenced by Sangon Biotech.