TT-TL-seq reveals transcriptional profiles that accompany DoG induction after hyperosmotic stress.
TT-TimeLapse-seq was used to investigate the nascent transcription profiles that accompany the induction of downstream-of-gene (DoG) containing transcripts in human cell lines. Data from HEK-293T cells exposed to hyperosmotic stress and cells transfected with siRNAs against Integrator subunit 11 are included. Overall design: Samples were treated with 80 mM KCl for 1 hour or with siRNAs for 48 hours. During the last 5 minutes of treatment, nascent RNAs were labeled with 1 mM s4U. After RNA extractions using TRIzol, 4% RNA from Drosophila S2 cells was added to each sample. TT-TL-seq preparations were done as described in Schofield et al. 2018.