Low-input Hi-C library construction
To evaluate the robustness of CtG, we constructed Hi-C libraries of varying quantities of cell inputs utilizing a low-input Hi-C technique. Overall design: Both HEK293 and HEK293T cells were used. Either 1,000 cells or 10,000 cells were extracted to a 0.5 mL Eppendorf Lobind Microcentrifuge tube. Hi-C experiments were performed followed the method described in Rao et al., 2014 (PMID: 25497547) with some modifications.