RNA seq of liver cancer cells treated with different concentration gradients of Thapsigargin (TG), Tunicamycin (TM), and FCCP
To investigate the effects of TG, TM, and FCCP treatments with different concentration gradients on liver cancer cells. Overall design: HCCLM3 cells were treated with TG (0, 0.5, 1, 2 µM), TG (2 µM) + melatonin (10 µM), TG (2 µM) + ISRIB (0.5 µM), TG (2 µM) + 4µ8c (20 µM), TG (2 µM) + MG132 (10 µM), TM (0, 0.5, 1, 2 µM), TM (2 µM) + melatonin (10 µM), TM (2 µM) + ISRIB (0.5 µM), TM (2 µM) + 4µ8c (20 µM), TM (2 µM) + MG132 (10 µM), FCCP (0, 1.25, 2.5, 5 µM), FCCP (5 µM) + 3-MA (5 µM), and FCCP (5 µM) + MG132 (10 µM) for 24 hours. Two biological replicates were prepared for each condition.