Phylotranscriptomics of terrestrial planarians
15 species of terrestrial planarians were hand-collected from Spain and Brazil. Sampled species. The samples were collected and stored in RNALater at <20ºC until processed. RNA extraction was performed using the TRIzol reagent (Invitrogen, USA) method following the manufacturer's instructions and adding 5 µg of RNase-free glycogen as a carrier to the aqueous phase for low input samples. Concentration of all the samples was assessed by Qubit RNA BR Assay kit (Thermo Fisher Scientific). Samples were subjected to Illumina's TruSeq Stranded mRNA library preparation kit and sequenced on a NovaSeq 6000 (Illumina, 2 × 150 bp) for a 6Gb coverage.