16sRNA sequence
B16-F10 cells were first injected into the right axilla of BALB/c mice to establish the model. When the tumor size reached approximately 100 mm3, the mice were randomly divided into two groups: the Anti-PD1 group (n = 6) and the Control group (n = 6) .Starting from day 0, the Anti-PD1 group received intraperitoneal injections of anti-PD1 antibody (RMP1-14): a single dose of injection given every two days apart for a total of 7 doses to induce myocarditis, while a single dose of mouse IgG injection was given every two days apart for a total of 7 doses intraperitoneally into the Control group. On day 14, fresh fecal samples were collected for16S rRNA gene amplification.