Gene expression profile at single cell level of WT and med22-F0-KO zebrafish embryo heads
We used single cell RNA sequencing (scRNA-seq) to identify genes differentially expressed in med22-KO embryos compared with WT, with the aim of identifying new pathways involved in the associated pathology. Overall design: Cells were extracted, isolated, and libraries prepared with the 10x Genomics manufactor kit, for sequencing using an Illumina Novaseq 60000