Single-cell RNA sequencing data of human craniopharyngioma tumor tissue
The craniopharyngioma tumor specimen was digested immediately after surgical resection, and a single-cell library was prepared using 10x Genomics. The obtained sequencing data were processed and analyzed using Cell Ranger. Overall design: The cell suspension obtained from the resected tumor specimens was processed according to 10×Genomics specifications, using the 10×Chromium Next GEM single-cell 3' reagent kit and library construction kit. The cell suspension was processed in the Chromium Controller to create gel beads in emulsion (GEMs), incubated at a specific temperature to generate cDNA containing barcodes and Unique Molecular Identifiers (UMIs). Subsequently, the cDNA was washed, amplified, and its quality assessed, then enzymatically fragmented and size-selected, followed by adapter ligation and indexed PCR amplification. Finally, after quantification and quality assessment of the library, sequencing was performed on the NovaSeq 6000.