MBD-Seq was applied to study the CpG methylation map of immortallyzed human myoblasts after DiPRO1/ZNF555 inhibition
The methylation enrichment was determined by MIRA-sequencing after MBD2-capture using the MethylCollector™ Ultra Kit (Active Motif) performed on DNA samples derived from immortallized human myoblasts. The cells expressing shRNA targeting ZNF555 (shZNF555) were compared to the control cells expressing non-targeting shRNA (shCtl). The signals of methylation-enriched DNA (ENR) were normalised to non-enriched input DNA (INP).