Vivaldi: An amplicon-based whole genome sequencing method for the four seasonal human coronaviruses, alongside SARS-CoV-2
We developed a 1200bp amplicon-based whole genome sequencing scheme targeting all four seasonal coronaviruses and SARS-CoV-2.The Vivaldi method was applied retrospectively and prospectively using Oxford Nanopore Technology to approximately 400 seasonal coronavirus infections diagnosed in Nottingham, UK, from February 2016 to July 2023.