Total and chromatin-associated RNA (totalRNA and chRNA) sequencing experiments upon rapid depletion of endogenous DHX15 or DHX38
To investigate DHX15's functions in splicing QC in human cells, we induced rapid proteolysis-mediated depletion of endogenous DHX15 and DHX38 and performed high-throughput sequencing of totalRNA and chRNA. Overall design: We generated two degron cell lines, HEK.dDHX15 and HEK.dDHX38 cells, tagging endogenous DHX15 or DHX38 gene copies with degron tags, and performed total- and chromatin-associated RNA-seq following 2 hours of DMSO or dTAG13 treatment.