RNA-Seq in HepG2-shNC and HepG2-shLINC01977 cells
Total RNA was extracted from HepG2 cells with sh-NC (n = 3) or sh-LINC01977 (n = 3). RNA samples were analyzed by RNA sequencing based on the manufacturer's protocols. Briefly, Illumina HiSeq 4000 platform was used to sequence the RNA samples for the subsequent generation of raw data. R package was utilized to select genes with significantly differential expression based on fold change =2.0 and P=0.05 between sh-NC and sh-LINC01977 cells. KEGG pathway and GSEA enrichment analysis were used for functional pathway analysis. Overall design: mRNA profiles of HepG2-shNC and HepG2-shLINC01977cells