RNA Sequencing of wound tissue of dbdb mice treated with PBS or MFGE8-Fb
Full-thickness excisional wounds (6 mm in diameter, extending through the panniculus carnosus) were created on the dorsal skin of eight-week-old mice using a biopsy punch. Mice were housed individually throughout the experimental period. To evaluate the therapeutic effect of MFGE8-pretreated fibroblasts on diabetic wound healing, mice were given a single injection at the wound edge on the day of wounding with 100 microliters PBS or 100 microliters PBS containing NIH/3T3 cells (1 x 10^7 cells/mL) pretreated with 1 microgram/mL MFGE8 protein for 24 h.