Effects of the absence of ACLY or Pdca1 on the transcriptome of T cells activation
T cell activation is known to require a metabolic shift towards glycolysis, triggered by TCR-engagement . To evaluate the role of ACLY or PDH in transcriptional reprogramming. T cell-specific Pdha1 knockout mouse by crossing Pdha1fl/fl mouse with CD4CreERT2 and CD4+ T cells from conditional knockout mice with T cell-specific ablation of Acly (CD4-Cre Aclyfl/fl). Overall design: mice  CD4+ deficients of ACLY or Pdha1cells were activated with CD3/CD28 during 24h