Regulation of optic nerve regeneration by srebf2
Laser capture microdissection of the retinal ganglion cell layer followed by mRNA-seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control, nonspecific morpholino control, morpholino mediated srebf2 gene knockdown, nonspecific morpholino control plus daily heat shock in Tg(hsp70l:nSrebf2-2AmCherry, cmlc2:EGFP) fish, and morpholino mediated srebf2 gene knockdown in Tg(hsp70l:nSrebf2-2AmCherry, cmlc2:EGFP) fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.