Making deep mutational scanning accessible: a cost-efficient approach to construct barcoded libraries for genes of any length
Recent developments in DNA synthesis and sequencing have allowed the construction of comprehensive gene variant libraries and their functional analysis. Achieving high-replication and thorough mutation characterization remains technically and financially challenging for long genes. Here, we developed an efficient, affordable and scalable library construction approach that relies on low-cost DNA synthesis and standard cloning technologies, which will increase accessibility to systematic mutational studies and help advance the field of protein science.