rhizosphere microbiome
Two varieties of upland cotton with different levels of resistance were selected: Zhongzhimian 2 (Zhong 2, resistant to VD) and Xinluzao 36 (Xin 36, highly susceptible to VD). Two varieties were cultivated in a random arrangement in the Verticillium wilt disease nursery at the Shihezi Academy of Agricultural Sciences, Xinjiang. The field has a continuous cotton growing history of more than 20 years, with the serious and uniform incidence of Verticillium wilt. In April 2021, the seeds of two varieties were sown in the field, and 18 plants (9 healthy + 9 diseased plants for each variety) were uprooted randomly with shovels when plants infected with VD showed obvious disease symptoms in August 2021