Study on the effect of agitative motion on syntrophic acid degrading enrichment cultures
The study aims to investigate the impact of disruptive motion, specifically orbital shaking and magnetic stirring, on metabolic activities, degradation rates, and community composition of syntrophic acid-oxidizing cultures grown in high ammonia conditions (0.3M).The culture was grown batch-style subjected to either no-motion, orbital shaking and magnetic stirring and fed either 50mM acetate or 50mM propionate. Each experimental condition was run in triplicates, resulting in the following samples:CA1-4 - No motion, Acetate, replicate 1-3CP1-4 - No motion, Propionate, replicate 1-3StA1-4 - Magnetic stirring, Acetate, replicate 1-3StP1-4 - Magnetic stirring, Propionate, replicate 1-3ShA1-4 - Orbital shaking, Acetate, replicate 1-3ShP1-4 - Orbital shaking, Propionate, replicate 1-3Two types of sequencing was performed during the study and uploaded to this project:16s gene amplicon sequencing:Sampled periodically throughout the degradation for both acetate- and propionate-fed cultures.metatranscriptomic sequencing:Sampled for both acetate- and propionate-fed cultures during the exponential phase of acid degradation, after substrate had been added a second time.The extracted RNA was merged for the acetate-fed replicates, resulting in a total of 3 samples(CA,StA,ShA), one for each motion. Propionate-fed replicates were not merged, resulting in a total of 9 samples (CP1-3,StP1-3,ShP1-3)