mRNA sequencing analysis during mouse trophoblast stem cells differentiation into trophoblast giant cells
We performed RNA-seq to determine the differential expression genes from undifferentiated trophoblast stem cells (TSCs) and 7-days differentiated trophoblast giant cells (TGCs). We performed RNA-seq to determine the differential expression genes from undifferentiated trophoblast stem cells (TSCs) and 7-days differentiated trophoblast giant cells (TGCs). mTSCs were nurtured in a stem cell medium  specifically designed for their growth. The differentiation of mTSCs into TGCs were enabled using a specialized differentiation medium for 7-days. This medium was essentially SCM without the components of FGF4 and heparin. Overall design: Undifferentiated mouse Trophoblast Stem Cells (mTSCs) and 7-days differentiated Trophoblast Giant Cells (TGCs)  were selected for RNA-Seq, in triplicate. Then, RNA extraction was performed using TRIzol® Reagent (Invitrogen). And The library preparation was constructed by NEBNext Ultra RNA Library Prep Kit for Illumina (NEB).Final libraries were quantified by Agilent 2100 Bioanalyzer then deeply sequenced on the Illumina HiSeq 2500 platform.