Transcriptome profiling of METTL7B knockout versus parental AsPC-1 pancreatic cancer cells
This project supports RNA sequencing of human pancreatic ductal adenocarcinoma AsPC-1 cells in which METTL7B (methyltransferase-like 7B) was disrupted by CRISPR-Cas9, compared with parental cells. The study investigates how loss of METTL7B alters gene expression programs linked to cell proliferation and motility, organelle organization, and lipid metabolism in pancreatic cancer. RNA-seq was performed using two independent METTL7B-knockout clones to capture consistent transcriptional changes upon METTL7B depletion and to relate molecular profiles to functional phenotypes observed in the associated work.