ATAC-seq data for damaged hepatocytes treated by a 3C cocktail [ATAC-seq]
To better understand the mechanism by which 3C induced revitalization, we next conducted epigenetic analysis to identify differences in transcriptional regulation. ATAC-seq data revealed that hepatocyte-enriched TFs, such as Hnf4a, Hnf1b, Hhex, and Prox1, and genes associated with epithelial cell proliferation and liver development were located in loci with open chromatin, whereas markers associated with mesenchymal transition, cell cycle arrest, apoptosis and inflammation (e.g., Col1a1, Mmp2, Ccl28, CxCr4, Ncf2) were located in closed chromatin loci in the 3C group Overall design: Comparative chromation states of ATAC-seq data for damaged hepatocytes treated by a 3C cocktail (i.e., CID755673 50 µM, GSK429286A 2 µM, and ETC-1002 5 µM), or the solvent control (DMSO) at day16.