Arabidopsis mRNA export factor tho5ab: molecular interactions and role in heat stress responses
A central function in linking RNA polymerase II transcription and pre-mRNA processing with mRNA export is executed by the transcription and export (TREX) complex. TREX consists of a hexameric core termed THO that associates with various combinations of additional proteins giving rise to alternative versions of TREX. Cell fractionation in combination with transcript profiling demonstrated that THO5 a subunit of the THO complex  is required for export of a subset of mRNAs . Overall design: To investigate export defects in tho5ab mutants upon heat stress RNA was isolated in nuclear and cytoplasmic fractions at 21° C and 37° C for 3h from wild type Arabidopsis Thaliana (Col-0) and tho5ab mutants