Transcriptome sequence of intestinal tissues with LL and HH modes in the murine whole-body irradiation model
To explore the molecular signaling pathways underlying different dose-rate modes, the intestinal tissues of mice were utilized to perform transcriptome sequencing. The result revealed that low mean dose-rate and low instantaneous dose-rate irradiation (LL mode) and ultra-high mean dose-rate and ultra-high instantaneous dose-rate irradiation (HH mode) evoked differential signaling pathways. Compared with LL mode, the signaling pathways of immune response were activated by HH, whereas the signals of mitochondrial metabolism and biogenesis, biological oxidation, amino acid metabolism and biosynthesis were suppressed under HH mode. Overall design: Female C57BL6/J mice were subjected to 10 Gy whole-body irradiation using LL and HH modes. Mice were euthanized at 48h post IR and extracted intestines to perform transcriptome sequencing.