Gene expression of healthy airway epithelium and p.Sdel560 STAT3-epithelium (Job Syndrome patient) in homeostasis and after 1 hour infection with pseudomonas aeruginosa
To explore the mechanism that underlies the lung infection susceptibility in job syndrome patients, we derived primary airway basal stem cells from a job syndrome patient which harbors a p.Sdel560-STAT3 mutation. After ALI differentiation, healthy airway epithelium and p.Sdel560 STAT3-epithelium were exposed to pseudomonas aeruginosa for 1 h. Then we collect the RNA to perform bulk-RNA sequencing. We then performed gene expression profiling analysis using data obtained from RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for healthy airway epithelium and p.Sdel560 STAT3-epithelium (Job Syndrome patient) in homeostasis and after 1 hour infection with pseudomonas aeruginosa.