Next Generation Sequencing facilitates to identify the differential expression genes of expanded MAIT cells upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA
We enriched expanded MAIT cells in PBMCs from healthy donor upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA through flow sorting  to perform next-generation sequencing (NGS).  Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with illumina  nova seq and sequence analysis was determined using the Illumina data analysis pipeline. Overall design: Differential gene expression between expanded MAIT cells,expanded MAIT cells with GCDCA,expanded MAIT cells with TCDCA,expanded MAIT cells with UDCA.