Genome-wide identification of transcriptional targets for H4K12 lactylation in ccRCC cells
To investigate the genome-wide transcription targets of H4K12 lactylation in ccRCC cells, cleavage under targets and tagmentation (CUT&Tag) was performed in 786-O and Caki-1 cells using antibody against H4K12 lactylation. Following CUT&Tag,  DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE. Overall design: CUT&Tag of H4K12 lactylation in  786-O and Caki-1 cells