Developmentally regulated isoform of HNF4a reprograms the adult liver
Hepatocyte Nuclear Factor 4a (HNF4a), master regulator of hepatocyte differentiation, is regulated by two promoters (P1 and P2). P1-HNF4a but not P2-HNF4a is expressed in normal adult liver in fed conditions. Both P1- and P2-HNF4a are expressed in fetal liver. P2-HNF4a expression is increased in fasted conditions, high fat diet, alcoholic liver and liver cancer. To determine the target genes of the P1- and P2-HNF4a isoforms, we compared P2-HNF4a-expressing exon swap mice (a7HMZ) to wildtype (WT) male mice. Liver ChIP-seq samples were taken at 10:30 AM (ZT 3.5) Overall design: Liver ChIP-seq samples from adult WT (P1-HNF4a1) and a7HMZ (P2-HNF4a7) male mice were analyzed in triplicate at 10:30 AM (ZT 3.5).