Integrating m5C Methylation Sequencing and Transcriptome Profiling to Investigate Key Genes and Molecular Mechanisms of Ginsenoside Rg1 Intervention in Head and Neck Cancer
This study by integrating m5C methylome and transcriptome profiling explores the therapeutic potential of ginsenoside Rg1 in head and neck cancer (HNC) through the lens of m5C RNA methylation and transcriptomic regulation, identifying key genes (THBS1, COL12A1, and ADAMTS1) exhibiting hypomethylation-high expression patterns in Rg1-treated FaDu cells. Functional enrichment analyses revealed their involvement in critical biological processes such as DNA replication, ribosome biogenesis, and lysosomal function. Additionally, immune infiltration analysis highlighted Rg1-mediated promotion of naive B cells and identified ICOSLG and PDCD1LG2 as potential immunotherapeutic biomarkers. Drug prediction and molecular docking suggested pravastatin sodium and methylcellulose as promising therapeutic candidates. This research establishes a three-dimensional therapeutic paradigm combining natural compound therapy, epitranscriptomic regulation, and tumor microenvironment remodeling, offering novel insights for developing innovative HNC treatment strategies.