Activity of the pleiotropic drug resistance transcription factors Pdr1p and Pdr3p is modulated by binding site flanking sequences
The PDR network is an important regulatory system in yeast involved in the transport of various toxins and drugs out of the cell. The transcription factors Pdr1p and Pdr3p, key regulators of this network, bind to specific sequences called PDREs in the promoter region of PDR genes to control expression. However, the exact mechanism underlying the differences in the regulons of these proteins are not well understood. A combination of genomic occupancy profiling (CUT&RUN) and in vitro binding assays were employed to characterize the differences in sequence specificity between transcription factors. The results indicate that interaction between the transcription factors and PDRE sites is not solely determined by the core sequence, but also influenced by the surrounding regions. Overall design: Genome binding/occupancy profiling by high throughput sequencing