Glycolysis inhibition by 2-Deoxy-D-Glucose sustains a distinct cell pluripotency state in human pluripotency stem cells
To investigate the function glycolysis in the regulation of hPSC pluripotency, we processed glycolysis inhibition (2DG) treamtent in E8 medium for 5 cell passages in H1/H9 ESCs and NL1/NL4 hiPSCs. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 2DG treatment for 5 cell passages in H1/H9 ESCs and NL1/NL4 hiPSCs