A study on the impact of the absence of the NIP or NrpR gene on gene expression of Streptococcus salivarius K12 (SAL)
NIP and NrpR forms a pair for the transcriptional regulator in Streptococcus salivarius K12 (SAL). To explore the target genes regulated by the NIP-NrpR pair in Streptococcus salivarius K12 (SAL), we generated mutants in which the NIP or NrpR gene has been knocked down through complement recombination. Comparative  transcriptome profiling of WT, ?nrpR, and nip* mutant strains revealed that the sar BGC is the major regulatory target controlled by the NIP signaling pathway in SAL. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different samples at the exponential growth phase of SAL. Comparative gene expression profiling analysis of RNA-seq data between wild type and mutant, total RNA was isolated from 3 independent growths (3 biological replicates)