Nanopore Amplicon-Seq of Glutenin Allelic Variants in a Wheat Collection
High-molecular-weight glutenin subunits (HMW-GS) are a polymorphic family of wheatstorage proteins encoded by three loci (Glu-A1, Glu-B1, and Glu-D1), located on thelong arms of chromosome one of the A, B, and D subgenomes, respectively. EachGlu locus includes two orthologous genes encoding a large molecular weight x-typesubunit (Glu-A1x, Glu-B1x, and Glu-D1x) and a lower molecular weight y-type subunit(Glu-A1y, Glu-B1y, and Glu-D1y). In this project, we performed PCR amplification of the six HMW-GS genes including promoter and CDS regions in a wheat collection (24 accessions) followed by Nanopore sequencing of the PCR products.