Transcriptome profiling of the liver and white adipose tissue of DIO mice treated with halofuginone.
This study is focused on evaluating the effects of halofuginone, administered at a dosage of 100 µg/kg, on gene expression changes in the liver and white adipose tissue of diet-induced obese (DIO) mice. Halofuginone is a bioactive monomer derivative obtained from the traditional Chinese herb Dichroa febrifuga. Previous studies have predominantly highlighted halofuginone's pharmacological actions by inhibiting EPRS, which subsequently activates the amino acid starvation and integrated stress response pathway. By examining differentailly expressed genes in both metabolic tissues, we aim to uncover the comprehensive effects of halofuginone on liver and adipose tissue metabolism. This investigation is intended to provide a foundational understanding of the intricate relationship between the amino acid starvation pathway and overall metabolic processes. The outcomes of this study are expected to shed light on how halofuginone influences metabolic health, thereby laying the groundwork for future therapeutic strategies targeting metabolic disorders through the modulation of amino acid starvation responses. Overall design: Mice were intraperitoneally injected with 100 µg/kg halofuginone or vehicle every two day. Samples were collected after 8 weeks.