Tongji-Huaxi-Shuangliu Birth Cohort with longitudinal (PRJCA047604)
This cohort was established since 2017 in the Shuangliu Maternal and Child Health Hospital, Chengdu City, China. Pregnant women aged 18 to 40 years who came to the hospital for the first prenatal examination in early pregnancy (6-15 weeks of gestation) were recruited. Exclusion criteria included: (1) multiple pregnancy detected by ultrasound; (2) pregnancy by assisted reproductive technology; (3) self-reported major chronic diseases or infectious diseases (eg, prepregnancy diabetes, cardiovascular disease, cancer, syphilis, or HIV infection); and (4) unable to finish the questionnaire. all participants were asked to provide blood and stool samples at each visit. By June 2019, there were 6143 pregnant women enrolled in this cohort. 16s rDNA sequencing, ITS sequencing as well as metagenomics sequencing were performed for selected stool samples dependent on the objectives of specific project. The study was approved by the Ethics Committee of the Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). All participants signed written informed consent at the first prenatal visit.