SHAPE-MaP of SARS-CoV-2 5'-stuctural elements (SL1-8) and SL1-4 variants
SHAPE-MaP was performed on in vitro transcribed RNAs: the first 8 stem-loops of the 5'end of SARS-CoV-2 and SL1-4b RNA variants. This was done to confirm folding of the RNA and determine secondary structure of RNAs with deletions of certain stem-loops. Structure probing was also done on the 5'SE, SL1-4b, and SL1-4 in the presence of purified OAS1 to compare differences in reactivity when protein is present.