Transcriptomic analysis of pulmonary infiltrated immune cells from wild-type, Hhip+/- and Cd101-/- mice
Pulmonary infiltrated immune cells from wild-type, Hhip+/- and Cd101-/- mice lung tissues were isolated to check the transcriptional profiling in lung immuno-microenvironment regulation. To isolate immune cells from lung tissues, lungs excised from mice were cut into small pieces using scissors and enymatically digested with RPMI contained Collagenase/Dispase and DNase I. Lymphocytic cells were enriched by Percoll density centrifugation.