scRNAseq of subcutaneous MC38 tumors 14 days post injection CD64DTR WT/WT or KI/WT treated with diphteria toxin from d9 to d14
8 to 14-week-old CD64 WT (CD64WT) and CD64-hDTR (CD64DTR) mice were injected with 0,5x106 MC38 cells in 100 µL in Phosphate Buffer Saline (PBS) subcutaneously. Macrophage depletion started by intraperitoneal (i.p.) injection of 50 µg/kg of diphteria toxin (Merck, 322326-1MG) and maintained every 48h with 17,5 µg/kg of DT in PBS.  Mice were euthanized 24h after the last DT injection. Late depletion: DT at D9-11-13, analysis at D14. MC38 tumors from CD64DTR and CD64WT were harvested at day 14 d.p.i., and single cell RNA sequencing was performed with 10X technology (more details are provided in Supplementary material).