Spray-Induced Gene Silencing (SIGS) with dsRNA: Efficacy, stability, and processing in potato plant protection against Phytophthora infestans
The experiment aimed to determine if potato and P infestans effectively process exogenous dsRNA. Both whole potato plants and in vitro P infestans were treated with 10 µg of dsRNA. Total RNA was extracted with the mirVana™ miRNA Isolation Kit. Library preparation and sequencing was conducted by NGI Sweden, SciLifeLab using a NovaSeq 6000 SP-100 flowcell. sRNA sequencing analysis demonstrated that both plant and pathogen effectively process target dsRNA into sRNAs, producing distinct populations of sRNA.