Gene expression profile of ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes from the brain IV
We used bulk RNA sequencing to investigate the astrocytic signature isolated from dead-neuron-injected 8-month-old APOE4-cKO mice compared with APOE4-KI mice Overall design: ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using Smartseq2 RNAseq.