Effects of Estradiol and Fulvestrant on gene expression in steroid-deprived MCF-7 cells
We treated steroid-deprived MCF7 cells with DMSO (Vehicle),  1 nM 17b-estradiol (E2), 100 nM fulvestrant (Fulv), or a combination of fulvestrant and estradiol for 24 hours Overall design: We then performed gene expression profiling analysis using data obtained from mRNA-seq of 3 biological replicates.