Recapitulation of regional patterning using human induced pluripotent stem cell (iPSC)-derived mandibular prominence ectomesenchyme (mdEM)
To ascertain the induction of developmentally accurate mdEM, we sampled cell aggregates during regional patterning induction and compared gene expression patterns. Overall design: RNA sequencing profiles of 1231A3 iPSC-derived neural crest cell (NCC) aggregates (day 5), mdEM (day 9), distal cap (day 13), proximal-oral domain (day 13), and dental mesenchyme (day 17).