Bulk transcriptomic profiling of dermal macrophage subtypes
Viable mouse dermal macrophage (CD45+F4/80+SiglecF-) subtypes were identified by antigen expression combination of Ly6C, FRb and CD163 and sorted from dorsal skin of 7 week-old C57BL/6 female mice by FACS. Samples were paired within individual mice (5 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing. Overall design: Dermal macrophage subtypes were sorted by surface antigens by flow cytometry followed by transcriptomic profiling with bulk RNA-seq.