Saccharum Liguleless1 Base Editing Line Sequencing
The study aimed to introduce multiallelic CRISPR/Cas9-mediated base edits at the LIGULELESS1 loci in sugarcane (40+ copies present in var. CP88-1762) to highlight the potential of the A3A-Y130F cytosine base editor alongside both an SpRYCas9 nickase and a traditional SpCas9 nickase.