PC-9 WES after EGFR-TKI and pemetrexed treatments
Genomic DNA was extracted from the parental PC-9 and PC-9-derived cells after exposed to EGFR-TKI with or without pemetrexed in long-term periods. Agilent SureSelect Human All Exon V6 exon capture kit was used for library construction to generate 180-220 bp fragments. Paired-end sequencing was performed using Illumina Novaseq PE150.