In vivo CRISPR screens for Toxoplasma hyperLOPIT-unassigened proteins_1
We performed in vivo CRISPR screens of Toxoplasma targeting genes with hyperLOPIT-unassigened subcellular localisation during mouse infection. Overall design: This Unassigned_1 library targeting 295 genes which consisted 2944 gRNAs targeting hyperLOPIT-unassigened genes together with dispensable and essential control genes.