16S rRNA and ITS amplicon sequencing of two popular Chinese milk kefir grains
Two commonly used Chinese milk kefir grains were submitted to Shengong (Shanghai) for 16S rRNA and ITS amplicon sequencing. Genomic DNA was extracted using the E.Z.N.A. MagBind Soil DNA Kit (Omega, M5635-02) and quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher, Q32854). Bacterial 16S rRNA genes were PCR amplified using universal primers 515F and 909R with Hieff Robust PCR Master Mix (Yeasen, 10105ES03). Prepared libraries were purified and size-selected with Hieff NGS DNA Selection Beads (Yeasen, 12601ES56) prior to sequencing.