To find the differentially regulated genes between WT and E81Q, WT and Q62L group [RNA-seq]
The androgen receptor (AR) plays an important role in male-dominant hepatocellular carcinoma, and specific acquired somatic mutations of AR have been observed in HCC patients. Our previous research have established the role of AR wild type as one of the key oncogenes in hepatocarcinogenesis. However, the role of hepatic acquired somatic mutations of AR remains unknown. In this study, we identify two crucial acquired somatic mutations, Q62L and E81Q, contributing to tumorigenesis. By RNA-sequencing, we found the differential genes in the mutant group compared with the WT group. Overall design: We constructed an animal model of hydrodynamic transgenic mice containing AR mutations gene. The mice liver tumor tissue was subjected to CHIP-seq and RNA-seq,separately