Altered Cell Clusters and Upregulated Aqp1 in Connexin 50 Knockout Lens Epithemium
To characterize the heterogeneity and cell clusters of postnatal lens epithelial cellsLens epithelial cells exhibit an intrinsic heterogeneity of different cell clusters in regulating lens homeostasis and lens growth. Upregulated Aqp1 in Cx50KO lens epithelial cells suggests that both connexin 50 and AQP1 likely play important roles in regulating water homeostasis in lens epithelial cells. (LECs) and to investigate the downstream targets of connexin 50 (Cx50) in the regulation of lens homeostasis and lens growth. To determine differentially expressed genes (DEGs) in the connexin 50 knockout (Cx50KO) lens epithelial cells that shed light on novel mechanism underlying the cataract and small size of the Cx50KO lenses. Overall design: Single-cell RNA sequencing (scRNA-seq) of lens epithelial cells isolated from one-month-old Cx50KO and wild-type (WT) mice were performed. Please note that R2 mate file of the 'lens epithelium,cx50ko,biorep1' sample (paired-end) raw data files is corrupted and thus only R1 file is provided as raw data.