Single-cell RNAseq analysis of wild type and Socs3 cKO retinal immune cells
Myeloid SOCS3 deficiency leads to increased retinal neovascularization in an oxygen-induced retinopathy mouse model. To investigate the role of SOCS3 in the function of myeloid lineage cells, particular microglia during retinopathy, total immune cells were sorted from wildtype and myeloid Socs3 knockout mice with retinopathy applied for single-cell RNAseq analysis by 10X Genomics technology. Overall design: scRNA-seq was performed for immune cells isolated from retinas of 6 KO and 6 sex-matched WT mice with or without oxygen-induced retinopathy (at postnatal day 13), using FACS. Total 1,200~2,500 cells were sequenced to an average of 65,00-118,000 reads/cell.