Total Ribo depleted RNA-Seq Metavac®-RSV
Six serial passages of Metavac®-RSV (P+1 to P+6) were realized by infection of Vero cells monolayers in 24-well plates at low MOI (10-3-10-4) in fresh infection medium. Initial viral seed batch of Metavac®-RSV was used for infection of the first passage and supernatants harvested after 7 days of production were then used for following successive passages. Viral RNA sequencing was made from samples of supernatant of selected passages P+1, P+4 and P+6. Library preparation was performed from the RNA extracted from samples using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit, following the manufacturer's recommendations. The prepared libraries were sequenced on an Illumina NovaSeq platform, using a 2x150 bp paired-end configuration.