Spatio-temporal RNA sequencing (RNA-seq) was conducted on Anopheles funestus mosquitoes collected across Africa (Cameroon, Ghana, Uganda, and Malawi) between 2014 and 2021. Each pool comprised total RNA extracted from 10 mosquitoes.
In this study, we utilized a comprehensive RNA-seq framework to investigate spatio-temporal changes in gene expression linked to the escalation of insecticide resistance in Anopheles funestus populations across Africa.