Assessment of In Vivo Chemical Mutagenesis by Long-Read Sequencing
In this study, we evaluated whether long-read PacBio-based HiFi sequencing (HiFi seq), which has integrated error-correction, can detect de novo mutations induced by mutagens in C57BL/6 mouse tissues. The mutagens used were 7,12-dimethylbenz[a]anthracene (DMBA), procarbazine (PCZ), and N-propyl-N-nitrosourea (PNU), and the tissues sampled were kidney, liver, lung, and spleen.