The Expression profile of splenic/hepatic macrophages in RNaseT2 deficient mice
RNA stress caused by the loss of RNaseT2, which is localized in lysosomes, leads to macrophage accumulation in the spleen and liver. However, the underlying mechanism remained unclear. Here, we demonstrated that cell proliferation in Rnaset2 -/- mice is dependent on TLR13, an RNA sensor. In both organs, TLR13 was found to induce cell proliferation and survival signals. Notably, in the liver, the most accumulated Ly6Clow macrophages were found to resemble wild-type Kupffer cells. These cells were shown to exert hepatoprotective effects through the LXR-CD5L axis. Overall design: To examine the characteristics of splenic macrophages that accumulate in Rnaset2–/– mice, we isolated Ly6C low or Ly6C high splenic macrophages from wild-type and Rnaset2–/– mice using FACS sorting. Similarly, to examine the characteristics of hepatic macrophages that accumulate in Rnaset2–/– mice, we isolated Kupffer cells, Ly6C low or Ly6C high splenic macrophages from wild-type and Rnaset2–/– mice using FACS sorting. Subsequently, we performed gene expression profiling analysis by employing RNA-seq data obtained from macrophage samples.