Long-range genomic contacts and spatiotemporal chromatin landscape of human histone gene clusters at Histone Locus Bodies during the cell cycle in breast cancer [ChIP-seq]
Cell cycle controlled mechanistic remodeling of chromatin architecture at HLBs as non-membranous phase-separated nuclear domains represents a novel dimension to regulation of cell proliferation. Therefore, we sought to resolve a gap in our understanding of how 3D higher-order genomic organization supports the activation of multiple clustered histone genes. Our findings are consistent model that the HINFP/NPAT complex controls the formation and dynamic remodeling of higher-order genomic organization of histone gene clusters at HLBs in early to late G1 phase to support production of histone mRNAs in S phase. that the two principal histone gene regulators HINFP and NPAT are located at anchor-points for chromatin loops reflecting their obligatory functions in remodeling the spatiotemporal genomic organization at HLBs during the G1 phase to accommodate histone gene expression in S phase. Overall design: ChIP-seq for 5 histone modifications in 4 BRCA cell lines with 2+ replicates and matched inputs.