Transcriptomics integrated with targeted metabolomics reveals endogenous hormone changes in tuberous root expansion of Pueraria
The results of endogenous hormone assay of "Gange No.5" showed that the contents of growth hormones, cytokinins, jasmonic acid, salicylic acid and melatonin differed significantly in different periods. Differential metabolites were the most diverse and enriched in the biosynthetic pathway, metabolic pathway pathway, and tryptophan metabolic pathway of secondary metabolites in all three period comparison combinations (S1 vs S4, S1 vs S6, and S4 vs S6). Among the 42 endogenous plant hormones detected, SAs were the most abundant in the pre-expansion stage (S1), Auxins were the most abundant in the mid- to late-expansion stage (S4), JAs and CKs were the most abundant in the pre-expansion stage (S1), ABAs and ETHs were the most abundant in the mid- to late-expansion stage (S4), MLTs had a small amount of distribution in S1 and S6, and GAs only had a small amount of distribution in S6.