Gardenia jasminoides Raw sequence reads
Gardenia jasminoides are extensively grown in worldwide with large harvest, and its major medicinal ingredients are geniposide and crocins. Nowadays, researches on their accumulation and biosysnthsis-related enzymes to excavate remain unknown. In this study, the geniposide and crocin accumulation of G. jasminoides fruit at different developmental stages were clarified by HPLC. The highest cumulative amount of geniposide is 2.035 % in unripe fruit period, and the highest content of crocin with 1.098% in matured fruit period. Furthermore, transcriptome sequencing was performed. A total of 50 unigenes encoding 4 key enzymes related in geniposide biosysnthsis pathways was screened, and 41 unigenes encoding 7 key enzymes in the pathways of crocin was elucidated. It was found that the expression levels of differentially expressed genes of DN67890_c0_g1_i2 encoding GGPS highly related to geniposide biosynthesis, and DN81253_c0_g1_i1 encoding lcyB, DN79477_c0_g1_i2 encoding lcyE, DN84975_c1_g7_i11 encoding CCD highly related to crocin biosynthesis, which were consistent with the accumulation of geniposide and crocins content, respectively. The qRT-PCR results showed that the trends of relative expression were consistent with transcribed genes. This study gives insights for understanding the geniposide and crocin accumulation and biosysnthsis during fruit development in G. jasminoides.