PLETHORA transcription factors promote early embryo development through induction of meristematic potential
Given that PLTs are key transcription factors in meristems, we hypothesized that loss of activity fails to confer meristematic properties or 'stemness' to the first embryonic cells, leading to developmental arrest. We asked to what degree downstream PLT targets in meristems overlap with those in early embryos. We selected direct PLT target genes by overlaying existing PLT ChIP-seq and DAP-seq datasets. We extended this set with an additional PLT3-DAP experiment on young roots, because previous PLT-DAP data have only low read counts and used leaf material in which PLTs are typically not strongly expressed. Overall design: DAP-seq was performed on gDNA extracted from 5 dpg Col-0 roots. Two DAP experiments were performed on the same gDNA sample: a 3xFLAG-PLT3 DAP and a 3xFLAG-EGFP DAP (negative control).