Bulk RNAseq of BMDM-T cells co-culture system with or without tumor antigen and IL-1beta or IL-18
To investigate the transcriptome changes of BMDM-T cell co-culture system, spleen lyphocytes were cultured with WT or Tmem175-/- BMDMs in 1640 supplied with FBS, m-CSF, and IL-2 for 48 hours. Tumor antigens, IL-1beta (500 pg/ml), or IL-18 (500 pg/ml) were added during the co-culture period. At the end of the culture, cells were harvested for RNA seq. Overall design: Spleen lymphocytes from B16-F10-OVA tumor bearing mice were cultured with WT or Tmem175-/- BMDMs, which were used as control groups. The experiment groups were treated with B16-F10-OVA tumor cell antigen. For further interventions, IL-1beta (500 pg/ml), or IL-18 (500 pg/ml) were added during the co-culture period.