RNA-seq of mouse fibroblast-like synoviocytes (FLS) isolated from mice with collagen-induced arthritis (CIA) and naive littermates
Fibroblast-like synoviocytes (FLS) were isolated from the inflamed joints of mice with collagen-induced arthritis (CIA) or the joints of naive littermates to characterise gene expression changes in response to chronic inflammation. Cells were isolated at two times of day (zeitgeber time, ZT4 and ZT16) to characterise diurnal variation in inflammatory symptoms and responses.  FLS cells were isolated from joint digests based on Podoplanin expression prior to RNA extraction and library preparation for sequencing.