Whole exome sequencing of 4-NQO-induced Oral Cancer line 2 (NOOC2)
We treated C57BL/6J mice with 50 mg/mL 4-NQO-containing drinking water for 16 weeks. Then, we switched mouse cages to regular water and monitored the development of HNSCC. A histology-verified tongue squamous cell carcinoma was processed to produce single-cell clones. Each single cell-derived line was expanded and screened in C57BL6/J mice for tumor establishment. Most clones were spontaneously rejected except for two 4-NQO-induced Oral Cancer cell line 2 (NOOC2)