Ileal content 16S rRNA sequencing in high- and low-laying hens
This project focuses on high-laying and low-laying hens. Ileal content samples will be collected for 16S rRNA sequencing analysis to compare the composition characteristics, community diversity, and differential distribution of intestinal microbiota between the two groups. By identifying dominant bacterial taxa and potential functional changes associated with laying performance, the study aims to screen key microbial groups closely related to the high- and low-laying phenotypes. Furthermore, it seeks to clarify the relationship between intestinal microecology and laying performance in hens, thereby providing a theoretical basis for elucidating the mechanisms by which gut microbiota regulate production performance and for developing microecological intervention strategies.