16S rRNA gene sequencing data of soil bacterial communities from four agroecological cropping systems in the CA-SYS experiment (2018-2021)
This dataset comprises raw 16S rRNA gene amplicon sequencing reads (FASTQ files) generated from soil samples collected within the CA-SYS experimental platform (INRAE, France), a long-term field experiment designed to evaluate agroecological cropping systems under contrasting soil management practices without pesticide use. Samples were collected from 42 experimental plots over four sampling years (2018-2021). DNA was extracted from soil samples and the V3-V4 region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform (2 x 250 bp). The dataset includes sequencing reads corresponding to 640 samples for which sequence data were obtained. These data provide the raw basis for downstream microbial community analyses, including taxonomic assignment and diversity assessment.