Identifying new markers of angiogenic sprouting using transcriptomics: New role for RND3
To determine, using HUVEC in a 3D collagen model, gene expression in invading endothelial compared to non-invading at the single-cell level. Overall design: 2 individual HUVEC donors were collected for each experimental condition.  Total, non-invading and invading HUVEC were collected from a 3D collagen invasion assay for single-cell sequencing.