ZNF292 genome-wide occupancy during cortical interneuron development
Human embryonic stem cell (hESC) models of ZNF292 deficiency were generated, including a KRAB-only control and two CRISPRi knockdown models (ZNF-G1 and ZNF-G2). These hESC models were used to derived MGE-like ventral telencephalic neural progenitors (day 15; D15) and cortical interneurons (day 30; D30). CUT&Tag was utilized to define ZNF292 genome-wide occupancy at D15 and D30 during this process. Overall design: For each time point, 4 biological replicates generated from independent differentiations of hESCs were used to assess differences in ZNF292 genome-wide occupancy between control (KRAB) and ZNF292 CRISPRi-mediated deficiency (ZNF-G1 and ZNF-G2) using CUT&Tag for ZNF292