Whole-exome sequencing of PaTu-8988T and KP4 KRAS KO and intact clones
Whole-exome sequencing was performed on pancreatic ductal adenocarcinoma (PDAC) cell lines and derived clonal populations with CRISPR-mediated KRAS knockout to characterize coding-region variation and assess genomic differences between edited clones and intact controls. Sequencing of parental cell lines and multiple clonal derivatives was conducted to evaluate clonal heterogeneity and identify potential background mutations that may influence downstream functional studies.These data provide a resource for understanding genomic variation in PDAC cell line models with KRAS loss and may support studies investigating oncogenic KRAS signaling, clonal heterogeneity, and mechanisms of therapeutic response to KRAS inhibition in pancreatic cancer.