Transcriptome of Anthurium andraeanum under cold stress
The transcriptome of leaves from two Anthurium cultivars, Oregon (Elegang, E, cold tolerant) and Fantasy love (Menghuan, MH, cold sensitive) under cold stress were sequenced by Illumina Novaseq™ 6000. Sequencing generated a total of 129.44 Gb of raw reads and an average of 7.19 Gb of reads for each sample. The analysis showed differences of transcriptomes between the control and the cold treatment samples. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles inAnthurium cold tolerance.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the cold stress response of A. andraeanum. Overall design: The full-flowering plants were grown in the growth chambers for three days with a 12/12 h day/night cycle at 25?. For cold treatments, the plants were grown at 15? for three days, then the temperature was decreased to 6? for three days and then 4? for three days. The control plants were grown at 25? throughout the experiment. Leaves were collected at 25?(control/CK), three days after the treatments at 6? and 4? for RNAseq. Three independent experimental replicates were performed, each consisting of three independent biological replicates.