Compatibility of Calycosin-Tanshinone IIA improves Ang II-induced renal artery endothelial cell dysfunction through lncRNA-mRNA coexpression network
In the present investigation, an experimental model of endothelial cell injury was established by subjecting RRAECs to Ang II (5×10-7mol/L) for a duration of 24 h. Subsequently, this model was treated with a combination of Calycosin (3mg/L) and Tanshinone II (3mg/L). The changes in total ATP levels and autophagy function in RRAECs were evaluated using the ATP assay and dansylcadaverine (MDC) staining, respectively. Annexin V-FITC/PI staining and transwell assay were utilized to quantify the apoptosis rate and migration function of RRAECs. Moreover, the utilization of RNA-sequencing technology facilitated the identification of differentially expressed (DE) lncRNAs and mRNAs between various groups. Subsequently, a coexpression network between DE lncRNAs and mRNAs was constructed, followed by the implementation of GO and KEGG pathway enrichment analyses to elucidate the functional implications of the DE mRNAs interacting with lncRNAs within this network. Overall design: Full transcriptome sequencing of 1 cell type