A new DRB1*15:01 allele
Here we describe a novel DRB1*15 :01 allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following: A*11:01 A*24:02 B*07:02 B*18:01 C*07:01 C*07:02 DRB1*04:03 DRB1*15:01 New DRB4*01:03/01:134/01:124 DRB5*01:01 DQA1*01:02 DQA1*03:01 DQB1*03:02 DQB1*06:02 DPA1*01:03 DPA1*01:03 DPB1*04:01 ;*- HLA-DRB1*15 :01 : New allele differs from DRB1*15 :01 by one nucleotide substitution at position 254 of exon 3, where  base C is replaced by G  inducing amino acid change , so that  Ser is replaced by Arg. In fact, the  176th codon of HLA-DRB1*15:01 (AG coding for Ser ) becomes AGG coding for Arg. in this New DRB1*15 :01.