Transcriptomics was used to reveal the effect of exogenous nitric nitrogen on cucumber roots under low temperature stress (PRJCA042602)
This study aimed to elucidate the molecular mechanisms by which exogenous nitrate nitrogen modulates low-temperature tolerance in cucumber (Cucumis sativus L. Xintaimici) roots. Hydroponically grown seedlings were subjected to low-temperature stress (10/15 deg C) and three NO3--N treatments: 0 mM (Group A), 12 mM (Group B), and 24 mM (Group C). Root samples were collected at 4 and 12 days post-treatment for transcriptomic (RNA-Seq and qRT-PCR) and physiological analyses.