LIGHT induces psoriais and atopic dermatitis related genes in keratinocyte
LIGHT upregulated a varity of genes in the human keratinocytes that are found to be highly expressed in the patients with psoriais and atiopic dermatitis. RNA was isolated from 3 different replicates per group, using a Qiagen kit and subjected to RNA-seq analysis. Overall design: Human epidermal keratinocytes from neonates were purchased from ATCC (NHEK, PS-200-010, ATCC, Manassas, VA) and grown in Dermal Cell Basal Medium (PCS-200-030, ATCC). 3x105 cells/well were cultured in triplicates using a 6-well format plate and stimulated with predetermined optimal concentrations of human rLIGHT (100 ng/ml) for 48 hrs. Controls received PBS.