Idnetification of a new DRB1*16:01 allele in a french  voluntary bone marrow donor
Here we describe a novel DRB1*16 :01 allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following:  HLA-A*01:01, *68:01  B*39:01, *51:01 C*12:03,* 15:02 DRB1*11:01 DRB1*16:01New HLA-DRB3*02:02 DRB5*02:02 DQA1*01:02, *05:05  DQB1*03:01, *05:02 DPA1*01:03,*- DPB1*04:01,*04 :02  HLA-DRB1*16 : 01 allele differs from DRB1*16:01 by one nucleotide substitution at position 48 of exon 4 (position 700 of cDNA), where  base G is replaced by A , inducing Ser instead Gly (GGC>AGC).