ChIP-seq analysis of DNA binding profile for the DAP5(amino acids 1-451) in HEK293T cells.
This submission contains ChIP-seq data generated to map the genome-wide DNA binding sites of a truncated form of DAP5. HEK293T cells were cultured and transfected with the Flag-DAP5(1-451) plasmid. A control group was transfected with the DAP5(1-451) plasmid. Cross-linking and immunoprecipitation using anti-Flag affinity gel were performed according to the manufacturer's protocols of the ChIP Assay Kit (Beyotime). The DNA was extracted, sequenced, and subjected to RT-PCR with indicated primers. For ChIP-seq analysis, quality-controlled FASTQ files were aligned to the human reference genome (hg19). After removing duplicate reads, significant protein-binding regions in the ChIP-seq data were identified using MACS2. The BAM files were converted into BigWig format for visualization using the bamCoverage tool from deepTools. Peak annotation was performed using Homer, aligning the peaks to the human reference genome.