RNA-seq of H929 with RAD23A knockdown
This study contains RNA-seq data generated from H929 cells under shNC and RAD23A knockdown conditions. The dataset was produced to investigate transcriptional changes associated with RAD23A loss, including pathways related to DNA damage response, apoptosis, and cellular metabolism. Libraries were prepared using poly(A) mRNA enrichment with oligo(dT) magnetic beads, random-primed cDNA synthesis, and Illumina RNA-seq library preparation (insert size approximately 380 bp; paired-end). Sequencing was performed on an Illumina NovaSeq platform (paired-end 2x150 bp).