CUT&Tag of oxygen-induced retinopathy mouse retina
c-Fos and c-Jun form activator protein 1 (AP-1) transcription factors that regulate gene expression by binding to specific motifs on the genome. To investigate c-Fos regulating genes in OIR rod photoreceptor cells, we performed CUT&Tag subjecting normoxia P14 and OIR P14 retina using c-Fos, H3K27ac (enhancer maker), H3K4me3 (active promoter maker), and H3K27me3 ( gene repression maker) antibodies. Overall design: CUT&Tag DNA-sequencing (CUT&Tag) for transcription factor c-Fos, and Control IgG as well as Histone modification H3K4me3, H3K27me3, and H3K27ac.