scRNA sequencing of the small intestine lamina propria of laboratory and rewilded mice
In rewilded mice, we observed B cells in circulation with increased signs of maturation, alongside heightened germinal center responses within secondary lymphoid organs. Rewilding also expanded B cells in the gut, which was accompanied by elevated systemic levels of IgG and IgM antibodies reactive to the microbiota. Our findings indicate that exposing laboratory mice to a more natural environment enhances B cell development to better reflect the immune system of free-living mammals. Overall design: Laboratory and rewilded mice (C57BL/6J) were compared after 6-7 weeks. Small intestine lamina propria cells were harvested and 4 mice per condition were pooled and then used on individual lanes for sequenceing.