Studying sequence specificity of bottromycin A2 action by Ribo-seq
Bottromycin A2 is an antibiotic inhibiting bacterial translation through the interaction with EF-Tu translation factor and aminoacyl-tRNA. To determine, if the drug displays sequence-specificity of action we performed a ribosome profiling (Ribo-seq) experiment in Bacillus subtilis and found that BOT preferentially stalls the ribosomes having a glycine codon in the A-site.