TCF7L1 controls tuft cell differentiation
To study the functions of Tcf7l1 during gut development, we have ablated Tcf7l1 specifically in the intestinal epithelium using Shh-Cre. To determine transcriptional changes upon loss of Tcf7l1, we have isolated EpCAM-positive epithelial cells from mouse embryos using fluorescence activated cell sorting (FACS) and performed RNA-sequencing analysis. Overall design: Samples: Shhcre-Egfp:Tcf7l1lox/lox and wild type. Cell type: epithelial cells from the small intestine of mouse embryos. Stage of embryogenesis: embryonic day 13.5 (E13.5). Replicates: three biological replicates.