Immune-related ceRNA regulatory network of radiation-induced esophageal injury in rats
We randomly assigned 27 rats to three groups (0, 25 or 35 Gy; n = 9). For stable irradiation, rats were anesthetized with intraperitoneal injections of 10% chloral hydrate, and the fur on the cervicothoracic was shaved off. Rats were sacrificed 7 days after radiation and esophageal tissues were collected.Esophageal tissues collected from three rats were combined yielding one pool sample. Each group (0, 25 or 35 Gy) contained three pool samples.Each pool sample is used for lncRNA, mRNA, miRNA, cirRNA sequencing.