Transcriptional response to interferon beta and effects of deletion of a predicted enhancer
We test the effect of CRISPR knockout of a predicted interferon inducible enhancer within the first intron of the IFNAR1 gene (IFNAR1.L1M2a) on the transcriptional response of GM12878 cells to treatment with interferon beta Overall design: Clonal wildtype GM12878 lines and clonal CRISPR knockout lines lacking a predicted enhancer within the first intron of the the IFNAR1 gene were either untreated or treated with 10 units per ml of human interferon beta over a time course of 4, 12, and 24 hours, and then profiled by RNA-seq