Development of a transformed Pseudomonas aeruginosa PA-pUCP24-PhzM as a cell factory for the enhanced production of Pyocyanin, a drug of choice in aquaculture
Pyocyanin, a secondary metabolite produced by Pseudomonas aeruginosa is a drug of choice in aquaculture in lieu of anibiotics. Pyocyanin is normally produced in smaller quantities by P.aeruginosa. Considering the possibilities of its large scale application in the control of fish and shrimp diseases such as vibriosis enhanced production of pyocyanin is crucial. Here, an attempt has been made to develop a transformed strain of P.aeruginosa, PA-pUCP24-PhzM for enhanced production of pyocyanin. The new strain PA-pUCP24-PhzM was obtained by transforming the wild P.aeruginosa MCCB 117 using a Gibson construct of pyocyanin biosynthetic gene PhzM in pUCP24 vector.