Single-cell dissection of the genotype-immunophenotype relationship in glioblastoma
We induced de novo brain tumor in immunocompetent genetically engineered mouse models EGFRvIII, Nf1 and PDGFB KO mice of both genders. We isolated brain tumor cells at the end stage in these mice and performed scRNA seq on these cells to compare their functional differences. scRNA demonstrated the distinct Tumor, Myeloid and Lymphoid cell population. PDGFB sample shows the higher proliferation and Wnt5a ligand expression and Nf1 showed the more mesenchymal type. Overall design: Using immunocompetent de novo mouse models of GBM , we generated tumors in the right-frontal striatumto represent major subtypes of GBM including (Proneural-PDGFB driven), (mesenchymal-NF1 driven) and (classical-EGFRvIII driven). Each tumor subtype contains the relevant driver mutations found in their human counterparts. Brain tumor cells were isolated at the humane endpoint of these mice, and sc-RNA seq was conducted to compare their functional differences.