16S rRNA gene sequencing of bacterial communities during soybean straw composting inoculated with Bacillus based synthetic microbial consortium YB1
This study performed high-throughput 16S rRNA gene sequencing (V3V4 region) to investigate the succession of bacterial communities during aerobic composting of soybean straw with the inoculation of a novel Bacillus based synthetic microbial consortium YB1. Three treatments were set: control (CK), inoculated (In), and inoculated with mulching (InC). Samples were collected at days 3, 21, and 35 of composting. Sequencing data were used to analyze bacterial a diversity, B diversity, community composition, key functional genera, and their correlations with physicochemical parameters such as temperature, pH, EC, CN, lignocellulose degradation, and nitrogen transformation. The dataset supports the reproducibility of microbial community analysis and provides a reference for the application of synthetic microbial communities in agricultural waste composting.