Gut microbiota analysis of Rat gut metagenome exposed to sodium fluoride via 16S rRNA sequencing
Sodium fluoride (NaF) is widely used in dental care products and water fluoridation, but excessive exposure may cause damage to the liver and intestines. This study investigates the effects of chronic NaF exposure on the gut microbiota and its potential role in NaF-induced gut-liver injury. Fecal samples were collected from a rat model (Sprague-Dawley rats) exposed to NaF at concentrations of 25, 50, and 100 mg/L for 12 weeks. Full-length 16S rDNA was amplified and sequenced using the PromethION P48 sequencer (Nanopore). This dataset aims to characterize the alterations in gut microbial composition following NaF exposure, including changes in the relative abundance of specific bacterial taxa (e.g., Firmicutes, Romboutsia, Collinsella, and Bacteroides). The data are associated with a study that integrates network toxicology and in vivo experiments to explore the gut-liver axis mechanism of NaF-induced hepatointestinal injury, with a focus on inflammatory mediators such as IL-6, IL-1beta, and TNF-alpha. This dataset provides valuable resources for understanding fluoride toxicity and developing potential interventions targeting the gut microbiota.