16s rRNA sequencing of gut microbiota in Betta splendens
The gut microbiota can influence host behavior and physiology through the gut-brain axis; however, its relationship with aggression-associated hormones remains poorly characterized in fish. In this study, we investigated the interactive effects of gut microbiota and aggression-associated hormones in male Siamese fighting fish (Betta splendens) across different genetic backgrounds and social environments. Bacterial communities were profiled through 16S rRNA gene sequencing targeting the V3-V4 region.