Bifidobacterium infantis intervention modulates gut microbiota in HDM-induced chronic asthma mice
This project investigates the effects of Bifidobacterium infantis supplementation on gut microbiota composition in a house dust mite (HDM)-induced chronic asthma mouse model. Twenty-four 3-week-old female BALB/c mice were randomly assigned to control, HDM, and HDM+B. infantis groups (n=8/group). Fecal samples were collected for 16S rRNA gene sequencing targeting the V3-V4 hypervariable region, performed on the Illumina MiSeq PE300 platform. Raw sequences were quality-filtered and clustered into operational taxonomic units (OTUs) at 97% similarity using UPARSE v11. This study aims to characterize gut microbiota alterations associated with B. infantis intervention and their potential role in the gut-lung axis in asthma.