Genome-wide microsatellite discovery and population genetic analysis of Korean mandarin fish (Siniperca scherzeri)
This project aims to investigate the genetic diversity, effective population size, and population structure of Korean mandarin fish (Siniperca scherzeri) in Korea. Whole-genome resequencing was performed using the MGISEQ-2000 platform (MGI), generating 150 bp paired-end reads from three genomic DNA libraries for genome-wide microsatellite marker discovery. High-quality reads were processed and assembled de novo, and microsatellite loci were identified using MISA-web and further filtered for primer design. The selected markers were applied to assess genetic diversity, heterozygosity, inbreeding coefficients, and population structure across multiple wild, broodstock, and released populations. This dataset provides important genomic resources for conservation genetics and supports broodstock management and stock enhancement programs.