Gene expression profile at single cell level from Glycine max nodules and roots.
The nuclei of Glycine max root seedlings (6 days-old) and nodules (28 days-old) were isolated and the library consstruction was performed applying 10x Genomics tecnology. Overall design: The nuclei of the 6 days-old roots of Glycine max seedlings grown on B&D-N media and growth chambers in dark. For nodule sample, the 3-day-old seedlings were germinated between moist Whatman filter paper and were inoculated with 1 mL of B. japonicum suspension (OD600=0.1) on transferred 3-day-old seedlings. The nodules were collected after 28 days. For the nuclei isolation was used mechanical and chemical steps and the libraries were made following 10X genomics recommendations.  The pre-processing and processing of the datasets were performed with Cell Ranger, and Seurat.