Whole transcriptome sequencing using  Huh7 cells transfected with Dengue virus serotype  4 NS1 expressing plasmid.
To study the role of Dengue virus serotype 4  NS1 in modulation of host cell transcriptome we performed gene expression profiling using data obtained from  RNA- sequencing of  total RNA from 3  different samples: Huh7 cells (no transfection), Huh7 cells transfected with pTracer-SV40  empty vector and Huh7 cells transfected with pTracer-SV40 encoding dengue virus serotype 4 NS1  (pDENV4 NS1). Overall design: After 24 hours of transfection, cells were lysed and total RNA was purified. RNA samples were proccessed for whole transcriptome sequencing using   Illumina Platform, followed by comparative gene expression analysis.