Acclimation of Chlamydomonas to light stress is mediated through multiple transcription factors
RNA-sequencing of the CLIP-mutants:LMJ_RY402_130655 Cre06.g275350 ROC40LMJ_RY402_056442 Cre02.g094150 PPR9LMJ_RY402_143080 Cre05.g238250 ABI5LMJ_RY402_077262 Cre03.g153050 RKD1LMJ_RY402_162524 Cre06.g266850 GATA19LMJ_RY402_103642 Cre08.g383000 TOE2LMJ_RY402_151120 Cre16.g675100 COL2LMJ_RY402_073370 Cre03.g149400 RKD3CLIP wildtype CC-4533And the HY5 CAS9 mutantcrhy5 (CC-4349 background) Cre06.g310500 crHY5Wildtpye CC-4349And their respective wild-type.All microalgal strains were grown in three replicates photoautotrophically in high salt medium HSM (Harris, 1989) under light high CO2 conditions 70 - 80 micromol photons s-1 m-2 and air enriched with CO2 (5 CO2 v/v) , and then transfered to high light, low CO2 conditions (350 - 400 micromol photons s-1 m-2 and regular atmospheric air (0.04 CO2 v/v)).All mutants show a low NPQmax phenotype.