CRISPR Cas9 based analysis of novel virulence genes in Mycobacterium avium subspecies paratuberculosis
To establish effective control strategies for Paratuberculosis, a chronic wasting disease of cattle, this study aims to generate CRISPR Cas9 based mutants of Mycobacterium avium subspecies paratuberculosis (MAP) and to identify novel virulence factors by characterizing their roles in infection, intracellular survival, and disease development.