Reduced Representation Bisulfite Sequencing (RRBS) of hippocampus from Mtr-cKO and wild-type mice
This dataset reports Reduced Representation Bisulfite Sequencing (RRBS) of hippocampal tissue from 21-day-old Mtr-cKO and wild-type mice. Genomic DNA was extracted from hippocampus, quantified, and quality-checked prior to RRBS library preparation using the Diagenode Premium RRBS kit. Bisulfite conversion efficiency was evaluated using methylated and unmethylated spike-in controls. Single-end 75 bp sequencing was performed on an Illumina NextSeq 550 system. Raw FASTQ files and processed CpG methylation tables are provided. Differential methylation analysis was performed using methylKit (R), including filtering, normalization, CpG calling, DMR identification, and genome annotation. Overall design: Twelve hippocampal samples (6 Mtr-cKO and 6 wild-type controls) collected from 21-day-old mice were processed for RRBS. Genomic DNA was extracted and prepared following the Diagenode Premium RRBS kit protocol, bisulfite-converted, and amplified. Sequencing was carried out in single-end mode (75 bp) on an Illumina NextSeq 550. FASTQ files were trimmed, aligned, and used for CpG methylation quantification. Differentially methylated CpG sites and DMRs were detected using methylKit with FDR correction.