Genome-wide mapping of RNA polymerase active sites in DOT1L-depleted HCT116 cells by PRO-seq analysis
Precision nuclear run-on sequencing (PRO-seq) approach was adopted to evaluate the effect of DOT1L in regulating transcription and RNA polymerase active sites in HCT116 cells Overall design: Control HCT116 cells (shNC) and DOT1L-knockdown HCT116 cells (shDOT1L) were used in this experiment