Fecal microbiota analysis of antibody mediated blocking of ß7-Integrin and WD-induced MetS in lymphopenic mice
WD-induced changes in the fecal microbiota of RAG-2-deficient mice given an IgG isotype or FIB 504 monoclonal antibody against ß7 integrin by 16S rRNA gene amplicon sequencing. RAG-2-deficient mice originating from five litters were randomly assigned to control or treatment  groups, placed in single-cages and fecal samples were collected at timepoint 0 prior to starting WD and antibody treatment and subsequently,  after 4 weeks of WD and antibody treatment