Effect of thrombopoietin mimetic on the bone marrow stromal cell populations after radiation exposure
Thrombopoietin mimetic (TPOm) or JNJ-26366821 can promote the regeneration of hematopoietic stem and progenitor, endothelial progenitor, endothelial, and mesenchymal stromal cells in the bone marrow, resulting in an increase of survival of mice exposed to total body irradiation (TBI), a model of hematopoietic acute radiation syndrome. To further understand the activity of TPOm in regulating the phenotypic changes of bone marrow stromal cells after radiation exposure, a single-cell RNA sequencing (scRNA-seq) analysis was performed. C57BL/6J male and female mice were subjected to TBI at 6 Gy by X-ray irradiator. TPOm (1 mg/kg) or vehicle (PBS) was injected subcutaneously 24 hours after TBI. Normal mice and ones treated with TPOm were used as baseline controls. Ten days after TPOm injection, bone marrow cells were sorted and collected CD45-TER-119-CD31- (triple negative) cells for scRNA-seq. One male and one female per group were pooled for the sequencing.