High-throughput SNP discovery in the rabbit (Oryctolagus cuniculus) genome by next-generation semiconductor-based sequencing
In this study we applied a next generation sequencing technology (Ion Torrent PGM) to identify single nucleotide polymorphisms (SNPs) in the rabbit genome. Equimolar genomic DNA from different rabbit breeds was pooled and digested with two restriction fragments. Two reduced representation libraries were produced and sequenced on two different Ion Torrent 318 chips. A total of 6343257 reads were aligned to the rabbit genome (oryCun2.0).