MNase-seq data in 4 modified strains of Saccharomyces cerevisiae, treated or not with 10-Phenanthroline
We showed that the introduction of a 81bp DNA segment in phase genes leads to the generation of fuzzier nucleosome arrays, but neither to a significant change in the placement of the +1 and –last nucleosomes, nor to a coordinate sliding of nucleosomes. This suggests that irrespectively of other nucleosomes, the +1 and –last nucleosomes are placed in well-defined regions marked by sequence-dependent intrinsic and extrinsic factors, while the rest of the nucleosomes are placed based on periodicity considerations. Overall design: In order to test the effect of gene-length modification on Nucleosome periodicity in G1 synchronized cells, we performed MNase-seq in 4 modified strains of Saccharomyces cerevisiae containing 81bp (5'-GCGTGTTGTGTTTTCTCCGAGGAGAAACATTCAAATCTTGTGCTATGGCTTTGCCTACCGTCTGCGCCATCCATCTTTCGC-3') inserted in the middle of 2 selected genes in each strain. The 4 strains were then  treated or not with 10-Phenanthroline to test the effect of transcription on nucleosome periodicity. The exact position of the 81bp insertion can be found in the publication : An integrated machine-learning model to predict nucleosome architecture, Sala et al, Submited to NAR