Identification of novel human intermediate progenitor genes in cortical development
To identify the transcriptional signature of human IPCs (hIPCs), we isolated TBR2-positive IPCs from developing human neocortex. We utilized an antibody to mark intranuclear TBR2 in single-nucleus suspensions purified from developing human neocortex procured at the peak of neurogenesis at gestational weeks (GW)14-17, and sorted the TBR2+ nuclei (considered hIPC derived) from the TBR2- nuclei (considered non-hIPC derived). RNA-seq was performed to determine the expression profiles of hIPC-enriched genes.