Drosophila head and body expression
RNA was extracted from groups of 30–120 heads and 8–80 bodies of 3–6-day-old flies for each sex for 6 Drosophila species (5–8 strains per species; collected from diverse geographic locations). RNA-seq (with poly-A selection) was performed using the Illumina NextSeq 550 platform  with 150-bp paired-end reads.