Focal epilepsy human surgical samples
We identified diffuse lesions made of BRAF V600E-mutant CD34-immunopositive stellar cells in human samples resected to cure drug-resistant focal epilepsy. We performed single-nuclei RNAseq 5' 10X on three human brain samples (two BRAF mutant samples and one BRAF wildtype sample as control) in order to identify the molecular phenotype of CD34+ cells. Overall design: The three samples are: (i) as control, one BRAF wildtype medial temporal lobe epilepsy associated  hippocampal sclerosis (MTLE-HS), (ii) one BRAF mutant medial temporal lobe epilepsy associated  hippocampal lesion, (iii) one BRAF mutant epilepsy-associated  neocortical lesion