Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman mediated regulation of the highly conserved let-7 cluster during apoptotic processes
RNA extracted from Drosophila wandering L3 wing imaginal discs was 2S rRNA depleted and prepared for miRNA sequencing using the QIAseq miRNA Library kit. The experiment was performed on Pacman and Dis3L2 null mutants and their respective isogenic control lines with four replicates of each condition.