Small intestine of a mouse Metagenome
This study demonstrates that camel milk alleviates chronic fatigue syndrome in mice through remodelling the small intestinal microbiota and restoring gut-brain axis homeostasis. Camel milk enriched beneficial Lactococcus lactis while suppressing pro-inflammatory Helicobacter.sp species. These compositional shifts, coupled with increased luminal lactate that positively correlated with enhanced intestinal barrier integrity (reduced plasma D-lactate and LPS, restored villus architecture) and attenuated local inflammation . Resolution of gut inflammation translated to reduced systemic inflammation, normalized HPA axis function (ACTH, CRH, CS), and improved behavioural outcomes. Our findings establish that camel milk acts through the microbiota-gut-brain axis to break the self-perpetuating cycle of inflammation and neuroendocrine dysregulation in CFS, supporting its potential as a microbiota-targeted functional food.