Characterization of culprit-coronary artery miRNA expression in acute myocardial infarction male patients
Coronary artery disease (CAD) is the leading cause of mortality worldwide. We aimed to compare expression of miRNA in the affected artery of acute myocardial infarction (ST-elevation myocardial infarction) male patients versus healthy individuals (control). Blood samples were collected during coronary catheterization from proximal culprit coronary arteries aimed for the interventions or from a random artery in control samples. RNA isolated from serum was used for miRNA high throughput sequencing. Overall design: miRNA libraries were prepared from RNA samples of 16 control and 18 STEMI patients, using the QIAseq miRNA Library Kit (Qiagen).  Library concentrations were measured with Qubit dsDNA HS Assay Kit (Molecular Probes Life Technology) and the quality was assessed by Bioanalyzer and TapeStation systems (Agilent).  All miRNA libraries were sequenced using Nextseq 550 (Illumina) with a read length of 75bp. Differential expression analysis was performed using the Qiagen platform.