WNT_Destruction_complex Base editing Screens
We performed a base editing screen targeting key components of the WNT destruction complex, including CTNNB1, AXIN1, GSK3B, and APC, in eHAP1 cells to investigate mechanistic insights into WNT signaling. WNT pathway activity was monitored using a reporter system as the readout. Samples were sequenced on the Illumina NextSeq platform.