Full transcriptome analysis of the mechanism of tuberculosis ESAT6-Fc2a in alleviating allergic asthma in mice
In the early stage, an OVA-induced mouse allergic asthma model was constructed and treated with ESAT6-Fc2a fusion protein, which proved that ESAT6-Fc2a fusion protein could reduce the infiltration of inflammatory cells in the lungs and had a therapeutic effect on asthma. In order to further explore the mechanism of action, we carried out transcriptomics sequencing, screened out related genes, enriched them, and focused on pathways with significant differences. In the later stage, q-PCR experiments will be performed for verification. Overall design: There were three groups in this experiment, namely the normal group, the model group, and the treatment group (ESAT6-Fc2a). In order to make the study statistically significant, three samples in each group were labeled with N, M and E in the transcriptome.