Nitrogen fixing microorganisms in rice fields
Both straw addition and water management affect biological nitrogen fixation (BNF), but it is unclear how straw addition impact BNF under continuous or intermittent flooding irrigation in a rice cropping system. A 15N2-labelling chamber system placed in the rice field was used to determine BNF under straw addition with continuous or intermittent flooding irrigation for 90 days. The nitrogenase nifH DNA gene from the soils were amplified by qPCR, the community structure of diazotroph was studied by high-throughput sequencing of nifH DNA gene.