Identifying and understanding drivers of chemoresistance in small cell lung cancer
Using a patient derived model of small cell lung cancer, the KEAP1 gene was deleted using lentiviral expression of a vector expressing CAS9 and single guide RNA (sgRNA) targeting KEAP1. A control vector expressing model was also generated. RNA-Seq analysis was performed to compare gene expression in tumors growing in vivo, comparing KEAP1 deleted to control tumors under saline-treated and cisplatin/etoposide-treated conditions.