Genome-wide identification and annotation of multi-nucleotide variants in human reveal widespread functional differences compared to single nucleotide variants
To validate the impact of MNVs, we integrated miRNA expression from The Cancer Genome Atlas (TCGA) and focused on hsa-miR-585-3p in non-small cell lung cancer (NSCLC), as this miRNA exhibits relatively high expression and an MNV in its seed region caused a greater number of target gene changes. We set up four experimental groups (wild type (WT), SNV1, SNV2 and MNV) and a control group (NC).