RNA sequencing analysis of gene expression in the mPFC of stressed DBA mice given vehicle of KDM5C inhibitor
We performed high-throughput profiling of gene expression in the mPFC of stress-vulnerable DBA2/J mice subjected to subchronic and mild social defeat stress.DBA mice were subjected to 5-day of social defeat stress and then injected with KDM5C inhibitor (KDM5Ci) or vehicle. After behavioral tests, mice were euthanized for tissue collection. We then conducted the transcriptomics analysis. Our study provided insights into the understanding of the molecular mechanisms underlying stress susceptibility and provide the role of KDM5C in stress-induced behavioral changes. Overall design: DBA mice were subjected to 5-day of social defeat stress (smSDS). Total 3 groups were set up: non-stressed (NS_Vehicle), SA:ANH subtype of smSDS-exposed mice given vehicle (SA:ANH-Vehicle), and SA:ANH subtype of smSDS-exposed mice given KDM5C inhibitor (SA:ANH-KDM5Ci. Three replicates were used for each group.