RNA-seq for Global off-targets of various RNA base editor potentiated by 2xHRD
Various editors were co-expressed in HEK293T cells with the corresponding gRNAs (30/22 for CIRTS and bt1-RESCUE-S,30/26 for ceRBE and S32L14 for CURE, respectively) before on-target editing at the NRAS and off-target editing at the transcriptome quantified by NGS.