Transcriptional landscape of the developing human adipose tissue
We collected adipose tissue specimens during elective surgery (inguinal hernia, cryptorchism). Samples were proceesed for extraction of total RNA, using phenol:chlorophorm extraction. Next generation RNA sequencing were performed. Published article: https://www.mdpi.com/1422-0067/24/23/16706 Overall design: Samples are labeled as patient groups (UCP1 high, UCP1 low). The UCP1 high samples strongly expressed uncoupling protein 1 (UCP1) and were lacking the interferon regulatory factor 7 (IRF7) protein, while the UCP1 low samples were lacking UCP1 and had high IRF7 levels. We have shown previously that high IRF7 level is associated with obesity. Our aim was to identigy differentially expressed genes (DEGs), to understand molecular mechanisms and cellular processes that are affected by IRF7. Project number: F22FTSEUHT0137_HOMrgmzR