Medicago sativa strain:Golden queen Transcriptome or Gene expression
In the present study, more than 28 million sequencing reads (5.6 Gb of clean nucleotides) were generated by Illumina paired-end sequencing technology using from 15 different alfalfa tissue samples. In total, 40,433 unigenes with an average length of 803 bp were obtained by de novo assembly. Based on a sequence similarity search of known proteins, a total of 36,684 (90.73%) unigenes were annotated. Among the annotated unigenes, 14,415 (35.66%) and 8,522 (21.08%) unigenes were assigned to Gene Ontology (GO) and Clusters of Orthologous Group (COG) categories, respectively. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG) database indicated that 5,723 (14.15%) unigeneswere mapped to 261 pathways.