ChIP-seq analysis in HepG2-TAF2-V5 clone
Taf2 is a transcription co-factor and a member of TFIID complex. To better understand gene regulation by Taf2 we generated HepG2 cells stably overexpressing TAF2-V5. This clone was used for ChIP using control IgG or V5 antibody. We identified TAF2-V5 binding near the transcription start sites of 37 genes. Overall design: Cells were subjected to IP using IgG or V5 antibody following which DNA was isolated for library preparation and sequencing.