CAGE-Seq of human Monkeypox virus (hMPXV) isolate's transcriptome
The virus was isolated from index case (MPXV_NRL 4279/2022), during an active surveillance in response to outbreak in 2022 of Monkeypox infection in Prague. The isolate was propgataed on CV-1 cell line. The RNA-samples derived from a mixed time points of MPOX viral infection. We applied Cap Analysis of Gene Expression (CAGE) with cap- trapping method  to uncover the TSS distribution patterns at the examined genomic regions in MPOX using three biological replicates. The CAGE™ Preparation Kit (DNAFORM, Japan) was applied. The CAGE libraries were sequenced on a MiSeq instrument with v3 (150 cycles) and v2 (300 cycles) chemistries (Illumina). After mapping the reads with STAR to the ON563414.3 reference genome, the TSSr software was used to determine the 5' ends of mRNAs.