Identification of a new nul DPB1 allele a french  voluntary bone marrow donor
Here we describe a novel nul DPB1*02  allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*24:02, *32:01 B*07:02, *14:01 C*07:02, *08:02 DRB1*07:01, *13:01 DRB3*01:01 HLA-DRB4*01:01 DQA1*01:03, *02:01 DQB1*02:02, *06:03 DPA1*01:03,*-  DPB1*23:01, DPB1*02 :New HLA-DPB1*02 : New allele differs from DPB1*02 :01 :02 :01  by one nucleotide deletion at position 1 of exon 3 (position 365 of cDNA), where  base T  is deleted , inducing a frame reading shift with creation of STOP codon.