Complete chloroplast genome sequence and phylogenetic analysis of Chrysosplenium insularis J.E.Jang, K.H.Lee & H.Y.Gil (Saxifragaceae), an endemic Korean species
Chrysosplenium insularis J.E.Jang, K.H.Lee &amp; H.Y.Gil, belonging to the family Saxifragaceae, is a perennial herb. C. insularis is an endemic species of Korea, distributed in shaded habitats such as forests, forest wetlands, and riversides in the southern coastal regions of Korea, including Jeju-do and Gageo-do. However, its chloroplast genome structure and complete sequence have not yet been reported. Therefore, we determined the complete chloroplast genome sequence of C. insularis using genome sequencing, assembly, and annotation. The total length of the chloroplast genome was 152,689 bp with a GC content of 37.5%. It featured a quadripartite structure (a large single-copy region, 83,587 bp; a small single-copy region, 17,098 bp; and two inverted repeat regions, 26,002 bp each). It contains 131 genes, including 86 coding sequences (CDSs), 37 tRNA genes, 8 rRNA genes. Phylogenetic analysis of 79 CDSs revealed that C. insularis is closely related to C. alternifolium Linnaeus and C. japonicum (Maxim.) Makino. These results provide a molecular basis for phylogenetic and evolutionary studies of the genus Chrysosplenium and offer fundamental genetic data on the chloroplast genome of C. insularis, which will contribute to applied research in areas such as phylogenetic relationships and conservation.