Pan-cancer bioinformatics analysis of TNFRSF12A and functional study in colorectal cancer
Cancer has become a major public health problem in the world, however challenges such as drug resistance, metastasis and cancer recurrence continue to be unresolved issues. Therefore, it is important to find new cancer markers or therapeutic targets. Tn this study, we found TNF receptor superfamily member 12A (TNFRSF12A) upregulated in cancer and associated with a poor prognosis. TNFRSF12A knockdown inhibited growth, colony formation and migration of colorectal cancer cells, and overexpression TNFRSF12A promoted these behaviors of colorectal cancer cells. RNA-seq results showed that TNFRSF12A overexpression caused increased NF-?B signaling, transcriptional misregulation, and significant upregulation of BIRC3 gene, which may be the transcription target of NF-?B member RELA. Through BIRC3 knockdown in overexpressed TNFRSF12A cells, we proved BIRC3 is a key downstream of TNFRSF12A. Therefore, we speculated the existance of TNFRSF12A/RELA/BIRC3 regulatory axis in colorectal cancer. Overall design: Comparative gene expression profiling of RNA-seq data from doxycycline-induced TNFRSF12A overexpression DLD-1 cells (oeT) and corresponding control group (oeCtl).