Native Index Ligation-Based Targeted Degradome Sequencing (NIL-TDS) for High Precision Quantification of small RNA Slicing Activity
RNA interference (RNAi) is an effective natural process in which small RNAs mediate endonucleolytic slicing of complementary target mRNAs. Despite the potential of RNAi in human therapeutics and crop bio-protection, analytical platforms to quantitatively validate the slicing activities of small RNAs are lacking. Here we present NIL-TDS, a method that combines RNA-ligase mediated PCR with Nanopore Sequencing for rapid and highly accurate identification of sRNA-mediated slicing.