Whole-genome resequencing of natural populations (ecotypes) of Arabidopsis from east Africa
Total genomic DNA of 16 newly collected accessions was extracted from young leaves. DNA was extracted using the PTB protocol (Wales and Kislter, 2019). Modifications were made to the length of the digestion (24 hours and the amount of the elution buffer (25 µl). At least 4 µg of genomic DNA was used to construct pair-end sequencing libraries (PerkinElmer NEXTFLEX Rapid XP DNA-Seq Kit HT) which were sequenced on a NovaSeq 6000 S4 X platform (Illumina, CA, USA). On average, a total of 1.22 Gb data for each accession was used for further analysis.