Idnetification of a new nul A*01 in a french  volunteer bone marrow donor
Here we describe a novel  nul A*01 allele, identified in a french  volunteer bone marrow donor. The same Sequence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:    HLA-A*01  : New allele differs from A*01 :420N by one nucleotide substitution at position 131 of exon 4 (position 750 of cDNA), where  base C is replaced by G  . Translated proteine will include Gln instead His at position 226 but as A*01 :420N , it will be trancated and can not be expressed at cell surface.