GSAR RNA-Seq Raw sequence reads
In this study, adaptive laboratory evolution (ALE) was performed on the wild-type Pichia pastoris GS115 strain, yielding the acid-tolerant strain GSAR capable of withstanding pH 2.6. The key genetic elements responsible for the acid tolerance of GSAR were identified, and the GSAR strain was further applied to the neutralizer-free fermentation for the production of organic acids and full-length recombinant proteins.