Tomato DRS for diurnal resrearch
This study aims to analyze the RNA modification levels and PolyA tail length differences in tomato (Solanum lycopersicum) leaves under different light treatment conditions. The experimental design includes two sample groups exposed to a 24-hour light/dark cycle: one group subjected to 12 hours of dark treatment (dark period, 12 hours) and another exposed to 8 hours of light treatment (light period, 20 hours). Direct RNA sequencing (DRS) technology was used to analyze the samples from both treatment conditions, aiming to identify differences in RNA modifications and PolyA tail length at different time points.Experimental Design:Treatment Conditions:Dark Treatment Group: Leaves exposed to 12 hours of darkness.Light Treatment Group: Leaves exposed to 8 hours of light.RNA Sequencing Type: Direct RNA Sequencing (DRS) to capture differences in RNA modifications and PolyA tail length.Study Goal: To compare the RNA modification levels and PolyA tail length differences in tomato leaves under different light treatment conditions, in order to understand the transcriptional regulation mechanisms of plants under light fluctuations.Data Analysis Objectives:RNA Modification Level Differences: To analyze the changes in RNA modifications under different time points and light conditions.PolyA Tail Length Differences: To assess the PolyA tail length differences in tomato leaves under different light treatments, aiming to explore the post-transcriptional modification mechanisms regulated by light.