Transcriptomic effects of complete RB1 loss in human mesenchymal stem cells during osteogenic differentiation
We investigated the impact of RB1 allele dosage on mesenchymal stem cell differentiation. Human AMSCs with heterozygous RB1 mutation were subjected to CRISPR/Cas9-mediated knockout of the second allele. RNA-seq analysis was performed during osteogenic differentiation to identify gene expression changes specific to biallelic RB1 loss.