Genome mining for haemolysin biosynthetic gene clusters in Brachyspira species
By genome mining a biosynthetic gene cluster encoding for a haemolysin precursor and its modifying enzymes and exporters belonging to the class of linear azol(in)e peptides was identified in different Brachyspira species isolated from pig faeces. Differences in the predicted consensus amino acid sequence of this haemolysin between Brachyspira species with a strongly and a weakly haemolytic phenotype could be accountable for these different phenotypes.