Microbial diversity on AGS
The inoculated activated sludge, and the sludge at the end of each phase were collected and analyzed for microbial community composition using 16S rDNA gene sequencing technology. The analysis process includes DNA extraction, PCR amplification, and high-throughput sequencing. The results include OTU clustering, species community analysis, and alpha diversity analysis, which were completed using the Ovisen cloud platform.