Effect of Ginsdenoside Rb2 to brown adipose tissue
Ginsenoside Rb2 is one of components in Panax ginseng, which has been traditionally used to treat metabolic diseases. In this study, we investigated the function of Rb2 in BAT by intraperitoneally injecting the Rb2 into mouse. To examine the systematic changes by Rb2 in BAT, we performed RNA sequencing, and analyzed DEG and pathways. Our analysis identified 727 genes significantly altered by Rb2 treatment. These genes were linked with BAT activation pathway such as adrenergic signaling, cAMP signaling, PKA signaling and lipolysis. Interestingly, adrb1 and adrb2 were upregulated by Rb2 treatment, while adrb3 was not, suggesting that Rb2 modulates BAT activation via specific adrenergic receptors. To confirm these findings, we conducted molecular experiment and found that Rb2 activates lipolysis via ß adrenergic receptor – cAMP – PKA signaling. Specifically, Rb2 enhanced the phosphorylation of HSL and PLIN, which are well known target of PKA. Our study provides a better understanding of the molecular mechanisms underlying the effects of Rb2 on BAT and may have implications for the development of novel therapeutics for metabolic disorders Overall design: To investigate the function of Rb2 in brown adipose tissue, we intraperitoneally injected the PBS or Rb2 into male wild-type C57Bl6/J mice for two weeks. We harvested brown adipose tissues from injected mice and extracted RNA from three mice per each condition. We performed RNA-seq and analyzed differentially expressed genes and related pathways between PBS and Rb2