RNA-Seq study of small follicles and large follicles in Indian Ghoongroo pigs
To gain insight into the differentially expressed genes (DEGs) and signalling pathways regulating development of small follicles (SF diameter =3 mm to <6 mm) into large follicles (LF diameter =6 mm) in Ghoongroo pigs, a comprehensive transcriptome profiling of porcine follicles was conducted using NovaSeq600 sequencing platform. The DEGs were identified using DESeq2 with threshold of padj <0.05 and log2 Fold Change cut off 0.58. Functional annotations and bioinformatics analysis of the DEGs were performed to find out biological functions, signalling pathways and hub genes regulating follicular development and function. Overall design: Differential  gene expression profiling analysis of RNA-seq data for Large follicle (3) and Small follicle (2)