Spatial Transcriptomic profiling of day2 wounds and intact tissues of Hgfac WT and KO mice
Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. Our scRNA-seq analysis of Hgfac WT and KO wounds reveals that Aldh1a3 is downstream of the HGFAC system and Aldh1a3 is highly upregulated in wound-asociated epithelial (WAE) cells in responce to endoscopic wounding, suggesting WAE cells can potentially secrete retinoic acid into wound bed. To dissect the healing responce in the intestinal mucosa, we peformed spatial transcriptomics analysis. Overall design: Day2 colonic wounds and intact colon samples from Hgfac WT and KO mice embedded into O.C.T. were used for Visium spatial transcriptomics.