Bulk RNA sequencing of a human iPSC-derived tri-culture
We used a human induced pluripotent stem cell (iPSC)-derived tri-cultures, comprised of neurons, astrocytes, and microglia, to determine whether Bruton's tyrosine kinase (BTK) inhibition could attenuate differential gene expression induced by Fc receptor stimulation. Overall design: Bulk RNA sequencing was performed with a human iPSC-derived tri-culture, consisting of neurons, microglia, and astrocytes. Tri-cultures had been treated with an IgG stimulus and/or an inhibitor of Bruton's tyrosine kinase (BTK).