Effect of small molecule inhibitors on transcriptome of B6.Sst1S bone marrow derived macrophages stimulated with TNF and infected with virulent Mycobacterium tuberculosis
We investigated  candidate host-directed therapies for tuberculosis in vitro. BMDMs from tuberculosis  susceptible mice B6.Sst1S were stimulated with TNF and infected with Mtb.  The infected macrophage were treated with Rocaglamide A, ISRIB and JNK inhibitor SP600125 alone or in combinations to asses the inhibitors' effectas on macrophage transcriptome. Overall design: B6.Ssts1S BMDMs were stimulated with 10 ng/ml of TNF for 16h and infected with Mtb H37Rv at MOI=1. At 72h post infection total RNA was isolated and processed for RNA-seq