Transcriptional regulator SATB1 limits CD8 T cell population expansion and effector differentiation in chronic infection and cancer [RNA-Seq]
Gene expression profile of exhausted CD8+ T cell populations upon deletion of SATB1 in in chronic infection Overall design: Naïve CD45.2+ SATB1-deficient P14 cells (Satb1flex/flex/Cd8Cre, SATB1-KO) or control cells (Satb1flex/flex) P14 cells were adoptively transferred into naïve wildtype CD45.1+ mice and subsequently infected with LCMV-Docile. On day 21 of infection, spleens were harvested. Single cell suspensions were prepared and cells enriched for CD8 T cells. The different populations were sorted. DNA libraries were constructed and sequenced