Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: RNA-seq
This study investigates transcriptional regulation by TES (TEAD1 Epigenetic Silencer, an engineered epigenetic silencer factor targeting the YAP/TAZ-TEAD axis) in SNB19 glioblastoma cells using RNA-seq. TES was engineered by fusing the KRAB domain, the catalytic domain of DNMT3A together with DNMT3L, and the N-terminal 166 amino acids of TEAD1. RNA-seq was performed 48 hours after lentiviral transduction to identify differentially expressed genes upon TES expression compared to GFP control in three biological replicates per condition. TES expression induced widespread transcriptional repression of YAP/TAZ-dependent gene programs, with downregulated genes enriched for cell-cycle regulation and TEAD family transcription factor binding sites. Overall design: RNA-seq profiling of SNB19 glioblastoma cells expressing TES (a synthetic TEAD1-targeting transcriptional silencer) versus GFP control. Three biological replicates per condition (6 samples total).