Single cell profiling shows that murine forebrain neural stem cells reacquire a developing precursor state when activated to make adult-born neurons
In this study, we have utilized a single-cell RNA-Sequencing platform coupled with transgenic lineage tracing in order to follow the progression of different embryonic neural stem cell populations as they progress into adult dormant neural stem cells. We show that adult dormant V-SVZ neural stem cells of different embryonic origins share a common molecular signature and reacquire an embryonic precursor-like state when activated to make new neurons in the adult brain. Overall design: High-throughput single cell transcriptomic profiles of the E14 cortex + GE tissue (3 replicates), E17 V-SVZ, P2 V-SVZ, P6 V-SVZ (3 replicates), P7 V-SVZ, P20 V-SVZ, P34 V-SVZ and P61 V-SVZ using either Emx1Cre or Nkx2.1Cre transgenic C57BL/6J mice were generated by single cell RNA sequencing, using the 10X Genomics platform. Processed and raw data for 12 datasets are included as indicated.