Luymes Bank Sinkholes  Filtered (0.22 µm) Shotgun Metagenomes
The Luymes Sinkholes, located on the Luymes Bank northwest of Saba, represent a unique marine environment characterized by distinct biogeochemical gradients. Most noticeable is an acidic (pH ˜ 6.25) and suboxic (O2 < 60 µmol/kg) submerged 'lake' at ~220 m depth exhibiting extreme deviations from standard oceanographic patterns, including elevated inorganic carbon, nitrate, and phosphate, and a reduced temperature (20.3 ºC vs 26.4 ºC). To determine how the microbiome within the submerged acid lake adapted to these unique conditions, we collected 59 coupled samples of size-filtered planktonic microbes for shotgun metagenomics and solid-phase extracted dissolved organic matter for untargeted metabolomics from 12 different sites at various depths during a 10-day research cruise in December 2019.