The maternal X chromosome impairs cognition and accelerates brain aging through epigenetic modulation in female mice II
To compare gene expression between old C57BL6 female mice treated with either control lentivirus or CRISPRa+ lentivirus, we used a flurorescence-based cell sorting to collect neurons expressing GFP (from the sgRNA) from the hippocampus of the mice from each group.Then we performed gene expression analysis using data obtained from RNA-seq of the old neurons in the hippocampus of both groups. Overall design: Comparative gene expression profiling analysis for old C57BL6 mice treated with either control lentivirus or CRISPRa+ lentivirus. Each experimental group has 4 biological replicates