Effect of androgen; gonad- and androgen receptor on gene expression in mouse kidney and liver
To investigate mechanisms underlying renal sex differences, we performed whole kidney RNA-seq on wild type C57BL/6J mice (Jackson Laboratory) at postnatal 0, 2, 4, 8 and 79 weeks old. We also profiled wild type adult C57BL/6NCrl (Charles River Laboratory) and adult castrated, ovariectomized mice when the surgeries for gonadal removal were performed at the age of around 3 weeks old. Tissue specific Ar (androgen receptor), Tbx10 and Neurog2 knockout mice were maintained at F1 cross Arc/c floxed allele to Six2TGC/+ CRE strain. To examine the acute response of testosterone, we injected testosterone to adult castrated and ovariectomized mice and collected kidneys 24 hours later for bulk RNA-seq. To compare the mechanisms of Ar in regulating sex differences systemetically or locally in kidney and liver, we obtained bulk RNA-seq for kidneys and livers from ubiquitous, nephron-specific and hepatocyte-specific knockout of Ar by Sox2CRE/+, Six2TGC/+ and Albumin-CRE mouse strain respectively. Overall design: Total RNA were collected for RNA-seq from minced 1) kidneys of C57BL/6J (Jackson Laboratory) at postnatal age 0, 2, 4, 8 and 79 weeks old; 2) kidneys of wild type C57BL/6NCrl mice (Charles River Laboratory), and those underwent castration or ovariectomy at around 3 weeks old that were injected with testosterone or oil control at 8~11 weeks old; 3) kidneys of adult conditional knockout by nephron-specific CRE strain Six2TGC/+ for Ar, Tbx10 and Neurog2 ; 4) kidneys and livers from ubiquitous removal of Ar by Sox2-CRE strain; 5) livers of hepatocyte-specific removal of Ar by Albumin CRE for both sexes.