oxygen-independent pathway for synthesis of pyridine nucleotides
Evolution of anaerobic fungi involved acquisition of oxygen-independent pathways for synthesis of pyridine nucleotides and Coenzyme-AStrain IMX2300 (fms1delta NcpanD) reproducibly showed a lag phase of approximately 48 h upon its first transfer from SMD to SMDdeltapan, and grew exponentially thereafter at a rate of 0.34 h-1. To explore whether the lag phase of strain IMX2300 reflected selection of a spontaneous mutant, it was subjected to three sequential transfers in SMDdeltapan. A single-colony isolate, IMX2300-1 from the adapted population showed a specific growth rate of 0.34 h-1 in both SMD and SMDdeltapan (Table 1). Whole-genome sequencing did not reveal any differences with the non-adapted strain IMX2300