Cecal microbiota profiling by 16S rRNA sequencing in C57BL/6 mice fed extra virgin olive oil or coconut oil based high-fat diets
To investigate high-fat diet (HFD)-induced changes in the cecal microbiota as a potential mechanism underlying distinct metabolic effects, DNA was isolated from the cecal content of C57BL/6 mice fed either extra virgin olive oil (E-HFD) or coconut oil (C-HFD) diets for 1 week. 16S rRNA gene amplicon sequencing was performed to profile microbial composition and diversity. This study provides insights into diet-induced microbiome alterations in a short-term HFD intervention.