Sequencing results for mt-optoIDR constructs
This study designed mt-optoIDR constructs which can undergo liquid-liquid phase separation within mitochondria in live human cells. Each construct contains a mitochondrial targeting sequence (MTS), an intrinsically disordered sequence (IDR) fused to a fluorescent reporter (mCherry) and a light-sensitive protein (CRY2olig). IDR sequences were derived from mitochondrial proteins and a control sequence from the protein FUS. We confirmed the sequences of our constructs using nanopore long-read sequencing.