Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.
In order to comprehensively characterize bone marrow mesenchymal cells after myeloablation, single-nuclei RNA sequencing was performed on bone marrow adipocytes and bone marrow stromal cells isolated from sublethally-irradiated mice. Overall design: Collected bone marrow adipocytes and sorted CD45/Ter119/CD31- cells from the bone marrow of Plin1-creER; tdTomato mice 5 weeks after sublethal irradiation were kept frozen until nuclei isolation. Cells were resuspended and extracted nuclei for single-nuclei RNA-seq (snRNA-seq).