Pantoea ananatis genome sequencing
This project describes the genome sequencing and characterization of Pantoea ananatis strain SK-1, an environmental isolate recovered from the Shirakawa River in Japan. Although SK-1 has been previously shown to induce necrosis in onion tissues, its genome sequence and virulence determinants had not been characterized. A hybrid genome assembly using Oxford Nanopore and Illumina sequencing was performed to generate a high-quality genome. The study focuses on the identification and functional validation of virulence-associated gene clusters, including the HiVir cluster involved in onion tissue necrosis and the absence of the alt cluster associated with thiosulfinate tolerance. This work provides insight into the pathogenic potential of environmental P. ananatis isolates and their relevance to agricultural systems.