Short-term depletion of acetylcholine synthesis in Drosophila enteroendocrine cells
Bulk RNAseq from whole adult Drosophila melanogaster guts expressing either mCherry-RNAi (control) or RNAi against ChAT (Choline acteyltransferase) under control of a enteroendocrine-specific driver to assess the role of epithelial acetylcholine production in intestinal barrier function. Overall design: UAS-GFP, tubGal80ts, propero-Gal4 was used as a driver to induce mCherry (Bloomington line BL 35785) or ChAT (VDRC line v20183) RNAi-mediated knockdown in enteroendocrine cells. Knockdown was induced for 3 days by shifting adult mated female flies (5 days old) to the permissive temperature (29ºC). 4 replicates were included for each genotype, consisting of 25 dissected whole midguts guts each (crop, malpighian tubules and hindgut were removed).