Mus musculus Metagenome ABX
The objective of this study is to verify whether the functional realization of the improvement in physiological phenotype by medium-chain inulin depends on its interaction with the intestinal microbiota. Specifically, it aims to explore whether the treatment with inulin of a specific concentration and structure can stably enrich the core functional microbiota. The specific research contents are as follows: 1) Using 5% medium-chain inulin as a positive control, while re-validating its phenotypic regulatory effect, to determine whether the changes in the intestinal microbiota and metabolite profile caused by it are consistent; 2) On this basis, by using broad - spectrum antibiotics to block the process of medium-chain inulin regulating the intestinal microbiota, specifically to eliminate potential core strains responding to medium - chain inulin, to explore the necessity of the core microbiota and its metabolites driven by it in achieving biological functions. Subsequently, this study aiming to clarify the causal links in the mechanism of medium-chain inulin effects and to determine whether key microbial groups and their metabolites constitute the necessary or sufficient conditions for improving physiological phenotypes.