Genome-wide CRISPRi profiling reveals the essentiality of the PTS Lactose operon for Human milk oligosaccharide metabolization in Streptococcus pneumoniae
In this study, we validated and further characterized the conditions and the type of human milk oligosaccharides (HMOs) that allow the growth of S. pneumoniae. Using RNA-seq, we confirmed and refined the identification of genes involved in Lactose (Lac) and HMO metabolization.