Spatial transcriptomic profiles of prostate tissue obtained from a patient diagnosed with benign prostatic hyperplasia (BPH).
ST profiles reveals the alterations in epithelial cells in Benign Prostatic Hyperplasia. Overall design: ST was conducted using the Visium platform (10x Genomics). Cryosection from an OCT-embedded BPH tissue sample was placed on Visium spatial slide. The mRNA molecules bound by printed capture oligos, which contained spatial barcodes on the slide, were converted into cDNA. Subsequently, the cDNA was transferred from the slide for library preparation. Spatial libraries were constructed using the Visium Spatial Library Construction Kit (10x Genomics, PN-1000184), following the manufacturer's instructions. Finally, sequencing was performed using HiSeq X10 (Illumina) with 150 bp paired-end reads.