StrainCascade benchmarking isolates
Whole genome sequencing of 17 phylogenetically diverse bacterial isolates cultured from human gut samples, including stoma effluent, feces, and gut tissue, collected in Switzerland. Isolates span 17 species across five phyla (Bacillota, Actinomycetota, Bacteroidota, Pseudomonadota, Fusobacteriota) and were sequenced on the PacBio Revio platform using the SMRTbell HiFi plex prep kit 96 with 30-hour movie collection times. These data were generated as part of the benchmarking dataset for StrainCascade, an automated modular pipeline for high-throughput long-read bacterial genome reconstruction and characterization. The complete benchmarking dataset comprises 152 genomes including 135 publicly available sequences from NCBI SRA.