Cannabidiol restores hematopoietic stem cell stemness in mouse through Atf2-Lrp6 axis after acute irradiation
Single-cell RNA sequencing analysis demonstrated that the expression levels of genes associated with stemness along with Wnt and BMP signaling pathways were restored by cannabidiol treatment through the upregulation of Atf2, a transcription factor possessing multifunctional properties. Overall design: BMNCs from wildtype mice, mice treated with 6.0 Gy gamma-irradiation with or without CBD treatment were FACS-sorted and remixed for sc-RNA-seq