Imputation of historical horse genomes
Historical genomes can provide important insights into recent genomic changes in horses, especially the development of modern breeds. In this study, we characterised 8.7 million genomic variants from a panel of 430 horses from 73 breeds, including newly sequenced genomes from 20 Clydesdales and 10 Shire horses. We used this modern genomic variation to impute the genomes of four historically important horses, consisting of publicly available genomes from 2 Przewalski's Horses, 1 Thoroughbred, and a newly sequenced Clydesdale. Using these historical genomes, we identified modern horses with higher genetic similarity to those in the past and unveiled increased inbreeding in recent times. We genotyped variants associated with appearance and behaviour to uncover previously unknown characteristics of these important historical horses. Overall, we provide new insights into the history of Thoroughbred and Clydesdale breeds and highlight genomic changes in the endangered Przewalski's Horse following a century of captive breeding.