Effect of global translation initiation inhibition associated to glucose starvation on cytoplasmic lncRNAs levels
To analyze the effect of global translation initiation inhibition associated to glucose deprivation on cytoplasmic lncRNAs levels, we performed RNA-Seq using WT, xrn1-delta and upf1-delta cells grown in glucose-containing complete synthetic medium (CSM) and then shifted for 16 min in glycerol- and ethanol-containing medium. In parallel, control cells were maintained for the same time in glucose-containing CSM. For the WT strains, we also included a treatment of 15 min with CHX (100 µg/ml final concentration) or an equal volume of DMSO (Mock). Overall design: Strand-specific total RNA-seq (2 biological replicates per condition).