snRNA-seq of tumor-induced lymph nodes
Lymph nodes (LNs) are peripheral immune organs innervated by sympathetic and sensory nerves. In this study, whole-mount 3D imaging revealed that sympathetic nerve fibers, rather than sensory nerves, exhibited significant elongation and increased branching over time during tumor-induced lymph node enlargement (TLNE). Single-nucleus RNA sequencing (snRNA-seq) analysis demonstrated that throughout TLNE, fibroblastic reticular cells (FRCs) are activated and engage in neuro-related signaling pathways while secreting substantial amounts of hepatocyte growth factor (HGF).