Genome-wide characterization of fragile and resistant nucleosomes in response to cold stress in maize [MNase-seq]
Distinct nucleosome architectures decode key regulatory information related to various cellular processes. However, how fragile (Fra-Nus) and resistant (Res-Nus) nucleosomes, with sensitivity and resistance to MNase cleavage, respectively, are reprogrammed and their potential biological implications under cold treatment is completely uncharacterized in plants. To this end, we conducted MNase-seq with light, medium and heavy MNase digestion in combination with multi-omics data (RNA-seq, ChIP-seq, MH-seq and resequencing data) between COLD and CK. Overall design: MNase-seq with light, medium and heavy MNase-seq between COLD and CK. Chip-seq of H3K27me3, H3k4me3, H3k9ac between COLD and CK. RNA-seq between COLD and CK. MH-seq between COLD and CK.