STOMP-seq: early multiplexing for high-throughput SMART RNA-sequencing
The transcriptomic data of this BioProject supports the development of STOMP-seq, a method that extends SMART RNA-seq protocols, like Smart-seq2 and Smart-seq3, to include sample-identifying barcodes on the 5' end of each amplified transcript. These barcodes allow samples to be pooled immediately after reverse transcription, enabling a 12-fold multiplexing strategy that reduces liquid handling complexity and enzyme costs several-fold. STOMP-seq reduces protocol execution times four-fold while improving library complexity and coverage. To demonstrate that STOMP-seq makes possible new large-scale experimental designs, in particular population-scale sequencing projects, this BioProject furthermore contains a multi-generational study of gene-expression heritability carried out using STOMP-seq.