Erasure of DNA methylation in rat fetal germ cells is sex-specific and sensitive to maternal high-fat diet.
we tested the impact of a maternal HFD on embryonic germline DNAme erasure using a rat strain that expresses green fluorescent protein specifically in germ cells. DNAme was analysed by methyl-seq capture in germ cells collected from male and female F1 gonads at gestational day 16. Overall design: After running the classic pipeline of DNA methylation data processing, DNA methylation was extracted for each samples and each mapped locus, then this data were segmented into segments with at least 50bp and finally we performed DMR search and explored the segments. Re-analysed Samples PRJNA909769: GSM6801700 SAMN32093467 SRX18528715 GSM6801701 SAMN32093466 SRX18528716 GSM6801702 SAMN32093465 SRX18528717 GSM6801703 SAMN32093464 SRX18528718