silkworm larvae fed As-contaminated mulberry leaves from five cultivars (PRJCA046759)
The P50 silkworm strain used in this study was provided by the State Key Laboratory of Resource Insects at Southwest University (Chongqing, China). A total of 3,000 newly hatched silkworm larvae were randomly allocated into ten experimental groups (300 individuals per group). Each group was exclusively fed fresh leaves from one of five mulberry cultivars, which had been cultivated for six months in either As-contaminated tailings soils or uncontaminated control soils. The larvae were fed twice daily (08:00 and 18:00) under controlled laboratory conditions.