Interaction between MDA-MB-231 cells and M2 macrophages and drug intervention
MDA-MB-231 cells were co-cultured with M2 macrophages induced by THP-1 cells by exchanging culture supernatants. In addition, for MDA-MB-231 cells cultured alone, Toll-like receptor 4 (TLR4) inhibitor TAK-242, baicalin-loaded nanoparticles (BANP), and lipopolysaccharide (LPS) were used for intervention alone or in combination; for M2 macrophages cultured alone, BANP were used for intervention, and induced M0 macrophages were used as phenotypic controls. The two cell samples were collected and the mRNA transcriptome expression profiles in the cells were detected. Research objectives: (1) Analyze the secretion-mediated interaction between MDA-MB-231 cells and M2 macrophages; (2) Analyze the effect of LPS on MDA-MB-231 cells; (3) Screen the regulatory mechanism of BANP on the two cells.