Identification of a new DPB1 in a french  voluntary bone marrow donor
Here we describe a novel HLA-DPB1  allele, identified in a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*26:01 , *31:01 B*27:05, *40:02 C*01:02, *02:02 DRB1*01:01, *14:54 DRB3*02:02 DQA1*01:01, *01:04 DQB1*05:01, *05:01 DPB1*new, *01:01  HLA-DPB1 New allele differs from DPB1*04 :02 :01 :01 by one nucleotide substitution at position  149 of exon 2 (position  248 of cDNA), where  base C is replaced by T , inducing Leu instead Pro (54CCT>CTT, Pro>Leu)