Effect of feeding on gene expression in WT, raga-1, and rsks-1 mutant animals
After subjecting WT, raga-1, and rsks-1 (S6K) animals to this growth arrest, we compared them by RNA sequencing (RNA-seq) to a group that was released from arrest by feeding for four hours Overall design: To investigate the nutrient response on gene expression, we treated WT, raga-1, and rsks-1 animals with 4 hours of feeding at the L1 larval stage.