effect of CD44 overexpression on luminal type breast cancer cell MCF7
To further identify the downstream target of CD44-dependent lamellipodia formation during luminal type BrCa progression, we firstly established a stable cell line by transfection of CD44 cDNA into MCF7 cells which expresses low level of CD44. Then we used RNA-sequencing (RNA-seq) to compare mRNA expression profiles between the MCF7vector and MCF7CD44 cells. Overall design: We firstly established a stable cell line MCF7CD44 , and then we used RNA-sequencing (RNA-seq) to compare mRNA expression profiles between the MCF7vector and MCF7CD44 cells. There are 3 replications for each group.