Proliferation response to insulin, selenium and transferrin in Golden Tank Goby muscle stem cells
We established a robust protocol for long-term in vitro expansion of muscle stem cells (MuSCs) from Golden Tank Goby (Glossogobius aureus). MuSCs were maintained undifferentiated for up to 80 passages using an insulin-transferrin-selenium cocktail, confirmed by Pax7 staining and myogenic differentiation. Selenium, alone or with insulin, significantly promoted cell growth, while transferrin had no effect. With 10% FBS plus selenium, cells expanded ~500-fold in 25 days. This work highlights a scalable strategy for fish MuSC culture, offering a foundation for cultured fish meat development.