DNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
Whole genome sequencing was performed on E. coli BL21 (DE3) evolved at 25°C in pH 9 terrific broth media buffered with Tris-HCl (pH 9). The evolved E. coli was characterized and compared to the parent strain.