Escherichia coli Genome sequencing and assembly
This project performed high-quality whole genome sequencing and de novo assembly of tet(X4)-carrying Escherichia coli MG1655 strains, which were experimentally evolved under two conditions: without antibiotic stress and under sub-inhibitory concentration of doxycycline stress, using PacBio long-read sequencing technology. The complete finished genomes, including circular chromosomes and all associated plasmids (harboring the tet(X4) tigecycline resistance gene), were obtained to investigate the compensatory evolution mechanism of this multidrug-resistant E. coli strain under antimicrobial pressure. This reference-grade genomic data will support in-depth analysis of adaptive mutations, fitness cost compensation, plasmid stability, and the evolutionary trajectory of tet(X4)-positive E. coli MG1655 during long-term doxycycline exposure.