Identification of the Regulatory Network Governing Cold Tolerance During Soybean Germination through the Transcriptomic Characterization of a Chromosome Segment Substitution Line
Low temperature is a key factor affecting the geographical distribution, growth, development and yield of soybean. Low temperature stress can lead to a large amount of productivity loss during seed germination. Soybean seed vitality is a key trait affecting planting and germination. In this study, three germination characters of 205 soybean materials were evaluated, and a soybean chromosome fragment substitute strain was identified, which had high seed viability at low temperature. A total of 5 quantitative trait loci (QTLS) on 5 chromosomes were associated with seed viability at low temperature. Integration of fragment import and transcriptome analysis revealed 6 candidate genes. qRT-PCR verification was performed, which showed the same trend as the transcriptome. In combination with KEGG enrichment analysis, transcription inhibitor KAN1 Glyma.20G108600 affected the expression of several hormone-related pathway genes in seed germination through its effect on target gene auxin.