The role of Rv1476 in regulating stress response and intracellu-lar survival of Mycobacterium tuberculosis
The virulence of Mycobacterium tuberculosis (M.tb) is related to many factors, including intra-cellular survival, cell wall permeability, and cell envelope proteins. However, the biological function of M.tb membrane protein Rv1476 remains unclear. To investigate the potential role played by Rv1476, we constructed an Rv1476 overexpression strain and found overexpression of Rv1476 enhanced the intracellular survival of M.tb, while having no impact on the growth rate in vitro. Stress experiments demonstrated that the Rv1476 overexpression strain displayed increased susceptibility to different stresses compared to the wild type strain. Transcriptome analysis showed that Rv1476 overexpression causes changes in the transcriptome of THP-1 cells, and differential genes are mainly enriched in cell proliferation, fatty acid degradation, cyto-kine-cytokine receptor interaction, and immune response pathways. Rv1476 overexpression in-hibited the expression of some anti-tuberculosis-related genes, such as CCL1, IL15, IL16, ISG15, GBP5, IL23, ATG2A, IFNß, and CSF3. Altogether, we concluded that Rv1476 plays an important role in M.tb survival in macrophages. Overall design: Construct an overexpression strain of Mycobacterium tuberculosis Rv1476 and analyze the effect of the overexpression strain of Mycobacterium tuberculosis Rv1476 on the transcriptome of THP-1 cells.