Fermentation metagenome
Microbial samples were collected from the Control and Aeration-0.2 fermentation broths on days 0, 6, and 8. Total genomic DNA was subjected to amplicon sequencing targeting the bacterial 16S rRNA gene and the fungal Internal Transcribed Spacer (ITS) region. Based on the microbial community dynamics observed, samples from day 8 of the Control and Aeration-0.2 groups were selected for shotgun metagenomic sequencing to further elucidate their functional potential. Sequencing was performed on an Illumina NovaSeq platform.