Anser cygnoides Raw sequence reads
Normal RNA of goose. Sperm Mobility (SM) is an objective method for measuring sperm motility, and the genetic mechanism underlying this trait warrants further investigation. In this study, SM and other semen quality parameters were determined in 40 Zi geese (Anser cygnoides L.) ganders, which were divided into high or low sperm mobility rank (SMR) groups, with three ganders in each group. The ganders (HSM1, HSM2, HSM3, LSM1, LSM2, LSM3) and corresponding 15 females comprised a breeding flock to determine fertilization in each group. The ganders were then sacrificed by anesthesia, and the testes were harvested for tissue sectioning and structural observation. The total RNA was extracted from the testes, and mRNA, long non-coding RNA (lncRNA), and microRNA (miRNA) expressed in the testes were analyzed using high-throughput sequencing to identify genes and non-coding RNA and related signaling pathways.Total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies), and the concentration, quality, and integrity were determined using a NanoDrop spectrophotometer (Thermo Scientific). RNA sequencing libraries (mRNA + lncRNA) were prepared from 2 \u03bcg of total RNA with the following modification. Small-RNA libraries were constructed using the NEBNext Multiplex Small-RNA Library Prep Set for Illumina (New England Biolabs, Inc.) according to the manufacturer\u2019s instructions. Small-RNA libraries (miRNA) were analyzed for quality control, and the average size of the inserts was approximately 140-150 bp. The sequencing library was quantified using an Agilent high-sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent Technologies) and then sequenced on a NovaSeq 6000 platform (Illumina). The samples were sequenced on-line, and the image files were converted to Raw Data in FASTQ format using the software that comes with the Illumina HiSeq sequencing platform. Reference genome transcriptome sequencing was performed using paired-end sequencing, and small-RNA sequencing was performed using the HiSeq Single-End mode.