Analysis of ovarian genes in pubertal female goats
Background: Age at puberty is an important factor affecting goat fertility, with genetic factors playing a crucial role in the onset of puberty. To better understand the mechanisms underlying puberty onset in goats, RNA sequencing was performed to analyze ovarian genes.      Results: A total of 18,139 genes were identified in cDNA libraries, and 75 differentially expressed genes (DEGs) were identified, of which 32 were significantly up- and 43 were down-regulated in pubertal goats. Gene ontology enrichment analyses indicated that DEGs were mainly involved in "metabolic process", "signaling", "reproduction" and "growth". Further, DEGs were significantly enriched in 91 Kyoto Encyclopedia of Genes and Genomes pathways, including estrogen signaling pathway, steroid hormone biosynthesis, and cAMP signaling pathway. Bioinformatics analysis showed that PRLR and THBS1 were highly expressed in pubertal ovaries, and ZP3, ZP4, and ASTL showed low expression, suggesting their involvement in follicular development and luteinization.      Conclusions: To summarize, ovarian DEGs expression were investigated in our study. Further studies are warranted to comprehensively explore the functions of DEGs in goat puberty.