Transcriptome sequencing of mice brian
Sleep disturbance is very common in patients with diabetes while the molecular mechanism is poorly understanded. We use db/db mice to demonstrate a sleep fragmentation phenotype. To explore the neuronal and molecular mechanisms basis of age-related sleep fragmentation in db/db mice, we conducted a transcriptome sequencing in different region of brain in db/db mice with different ages.cortical (containing the cortex and hippocampus) and subcortical (containing the thalamus, striatum, hypothalamus, cerebellum and brain stem) brain regions were isolated from three pairs of db/db and WT mice at 2 and 3 months of age during the light phase of the diurnal cycle.