The effect on gene expression in filamentous haploid of sugarcane smut fungus Sporisorium scitamineum
There are two mating types, “+” and “-” strains in S. scitamineum. Generally, dikaryote cells are formed after fussion of the germ turbs from both mating type strains and filamentation (filamentous growth) with infection potential occur afterward. By introducing bE2 gene and/or orther related genes, derived from the opposite mating type strain JG36, haploid mutants with filamentation characteristics were generated,  including M6,M8,M9 and M10. Among these are 446 genes that were found in both filamentous haploid strains, including 237 genes up-regulated and 209 genes down-regulated. The up-regulated genes were mainly enriched in amino acid biosynthesis, starch and sucrose metabolism, secondary metabolite biosynthesis, and pentose phosphate pathways. Down-regulated expression genes are mainly enriched in fatty acid synthesis and metabolism, homologous recombination, peroxides, biosynthesis of unsaturated fatty acids, ubiquitin-mediated protein hydrolysis and other metabolic pathways. Several MAP kinases and protein kinases were up- or down-regulated in the filamentous haploid. Overall design: We generated filamentous haploids by ATMT. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild type strain and filamentous haploids. Comparative gene expression profiling analysis RNA-seq data for wild type strain and filamentous haploids.