16S rRNA gene sequencing of human gut microbiota modulated by Millettia speciosa by-product proteins covalently bound with polyphenols
This study performed high-throughput 16S rRNA gene sequencing to investigate the modulatory effects of Millettia speciosa by-product proteins covalently bound with polyphenols (tannic acid, quercetin, resveratrol) on human gut microbiota during in vitro fermentation. The goal was to reveal changes in microbial community diversity, composition, and function, and to evaluate the prebiotic potential of modified proteins. Raw sequencing data were generated using Illumina platform and deposited to support the reproducibility of related research on gut microbiota regulation and functional food development.