Targeted DamID of selected chromatin proteins in quiescent and proliferating neural stem cells
Targeted DamID of embryonic proliferating (epNSCs), quiescent (qNSCs) and larval proliferating neural stem cells (lpNSCs) was performed using worniu-GAL4, tubGAL80ts (for qNSCs and lpNSCs condition) or worniu-GAL4 (for epNSCs condition) as a driver to express nine different Dam-fusions and Dam on its own (Dam-Only) as control. The following Dam fusions were profiled: Dam-Brm, Dam-RNA Pol II, Dam-TAF3-phd (H3K4me3), Dam-Dnmt3a-pwwp (H3K36me3), Dam-15f11 (H4K20me1), Dam-19e5 (H3K9ac), Dam-HP1a, Dam-Pc, Dam-H1.