identifying all AR binding regions in PC346C cell line
The Androgen Receptor plays in general an important role in Prostate Cancer biology. The Androgen Receptor orchestrates as transcription factor the activity of AR-regulated genes at destinct genomic regions by binding to specific sequences (known as Androgen Responsive Elements - ARE). However, the biophysical nature of the Androgen Receptor at these binding sites on the DNA are not well characterized. In order to investigate the biophysical properties of these molecules at AR binding sites by super resolution microscopy in living cells, we performed a AR-ChIP sequencing for the PC346C target cell line. Based on the sequencing results, we selected certain target regions based on relative AR-enrichment for further studying the local AR dynamics in these target regions. Overall design: Chromatin immunoprecipitation followed by DNA sequencing for the wild type Androgen Receptor