Transcriptomics revealed sex differences in human pulmonary microvascular endothelial cells in normoxia and hypoxia
To investigate sex differences at the transcriptome level in human pulmonary microvascular endothelial cells (HPMECs) from healthy male and female donors basally (in normoxia) and in hypoxic conditions. RNA-seq was performed on male (n=3) and female (n=4) HPMECs that were cultured in conditions of physiological shear stress (PMID: 36730645) in normoxia (21% O2) or in hypoxia (1% O2) for either 24 or 48 hours. Overall design: RNAseq of normoxic and hypoxic (for 24 or 48 hours) male and female HPMECs