Single cell sequencing of mouse gammadelta T cells
Mouse gammadelta T cells were sorted from adult C57Bl/6 mouse spleen and lymph nodes and cells were subjected to scRNA-seq Overall design: Single cell suspensions from pooled spleens and lymph nodes were stained with viability dye, CD45, CD3 and gdTCR. Cells were gated on viable, CD45+CD3+gdTCR+ cells.