Microbial Communities in Leachate Contaminated Soil
This study analyzes the microbial communities in leachate-contaminated soil in Jalan Lipis Sanitary Landfill. The study also investigated how the heavy metal (Mn, Ni, As, Pb) concentration and pH influences the diversity and distribution of the microbial communities. This sanitary landfill received domestic waste from all over Lipis. The estimated area is about 10.22 ha and receives roughly about 51.45 tonnes of waste daily. The extraction of gDNA was done by using a GF-1 Soil sample DNA extraction kit (Vivantis Technologies, Malaysia). The bacterial 16S V3 hypervariable region was amplified using the universal bacterial primers (341F and 518R) with a partial Illumina adapter.