Bar-seq
This study aims to optimize and expand the application of Bar-seq (barcode sequencing), a high-throughput method for profiling gene function and chemical-genetic interactions using pooled mutant libraries. The study introduces a refined Bar-seq workflow compatible with multiple sequencing platforms (Illumina, MGI, Element, Oxford Nanopore), improving barcode amplification, sequencing, and analysis. By enhancing accuracy and scalability, this work enables broader applications of barcode-based counting in contexts such as drug response profiling, environmental perturbation studies, and retrotransposon tracking, providing a versatile tool for functional genomics research.