A new DPB1*03:01 allele in a french  voluntary bone marrow donor
Here we describe a novel DPB1*03 :01 allele, identified in a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*02:01, *29:02 B*07:02, *44:03 C*07:02, *16:01 DRB1*04:04, *07:01 DRB4*01:01, *01:03 DQA1*02:01, *03:01 DQB1*02:02, *03:02 DPA1*01:03, *02:01 DPB1*11:01, *03:01new HLA-DpB1*03 :01 New allele differs from DPB1*03 :01 by one nucleotide substitution at position  56 of exon 4 (position  702 of cDNA), where  base G is replaced by T , without amino acid change  (GTG>GTT).