Transcriptome profiling of Cannabis sativa seedlings under cold, salt, and combined abiotic stress during early seed germination
Strand-specific RNA-seq data from Cannabis sativa L. var. Qingdama 5 seedlings harvested at 48 hours post-imbibition under four treatment conditions: control (25 degrees C, distilled water), cold stress (4 degrees C for 12h then 25 degrees C), salt stress (200 mM NaCl, 72h), and combined cold and salt stress. Three independent biological replicates per treatment (12 samples total). Sequenced on Illumina NovaSeq 6000, paired-end 150 bp, approximately 40 million reads per sample.