Comprehensive transcriptional analysis of dorsal skin development in pigs
The skin is the body's first line of defense and has multiple functions; its development is a complex, multifactorial regulatory process. Porcine skin is physiologically, anatomically and histologically more similar to human skin, and is often used as a model animal for human skin research. There are fewer studies on the transcriptome aspects of the skin during the porcine embryonic period, and the data obtained in this study may help to explain the age-related changes in transcriptional patterns during skin development and provide further reference for understanding human skin development at the molecular level. In this study, RNA sequencing was performed on the dorsal skin of Chenghua sows at embryonic day 56 (E56), embryonic day 76 (E76), embryonic day 105 (E105), and 3 days after birth (D3). Exploring RNA changes in porcine dorsal skin at four ages. Expression profiles of messenger RNAs, long-stranded noncoding RNAs, microRNAs, and cyclic RNAs were analyzed. The biological functions of their differential genes were investigated by gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A number of skin-related differential genes were identified by intercomparison between RNAs at four time points, and KEGG functional analysis showed that these differential genes were mainly enriched in metabolic and developmental, immune, and disease pathways,and the pathways enriched in GO analysis were highly overlapping. Collagen is an important part of the skin, with type I collagen making up the largest portion. In this study, collagen, type I, alpha 1 (COL1A1) and collagen typeI alpha2 (COL1A2) was significantly upregulated at four time points. In addition, lncRNA-miRNA-mRNA and miRNA-circRNA co-expression networks were constructed. Overall design: RNA sequencing was performed on the dorsal skin of Chenghua sows at embryonic day 56 (E56), embryonic day 76 (E76), embryonic day 105 (E105), and 3 days after birth (D3). Exploring RNA changes in porcine dorsal skin at four ages. Expression profiles of messenger RNAs, long-stranded noncoding RNAs, microRNAs, and circular RNAs were analyzed. The biological functions of their differential genes were investigated by gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.