Shotgun metagenomic analysis of soil microbial communities under nitrogen amendment and biological nitrification inhibition (BNI) conditions
This project investigates the impact of nitrogen amendment and biological nitrification inhibition (BNI) on soil microbial communities using shotgun metagenomic sequencing. Soil samples were collected from controlled bioreactor systems under three conditions: unamended control, ammonium (NH4+) amendment, and NH4+ amendment with the BNI-producing plant Brachiaria. The study aims to characterize shifts in microbial community composition, with a particular focus on methanotroph populations and functional genes associated with methane oxidation and nitrogen cycling. Results from this dataset provide insights into how BNI influences coupled carbon and nitrogen processes in soils, including potential tradeoffs between nitrification suppression and methane oxidation capacity. These data contribute to understanding the ecological impacts of BNI on greenhouse gas dynamics in agricultural systems