Gene expression profile at single cell level of adipocyte progenitors from subcutaneous inguinal adipose depots
Stromal vascular fraction (SVF) derived from adipose tissue is enriched for adipocyte progenitor cells, it is composed of aheterogeneous populations of cells and cell types. We use single-cell RNA-Sequencing (scRNA-Seq) to deconvolution of cell types in adipocyte progenitors and a general hierarchical structure is emerging. Overall design: Adipocyte progenitors (CD31- CD45- SVF) of C57BL/6J mouse were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of CD31 and CD45 and analyzed using scRNAseq.