RNA-Seq of Arabidopsis Col-0 and Dexamethasone (DEX)-inducible HopF2 Pto seedlings
Plant pattern-recognition receptors (PRRs) serves as surveillance system detecting microbe/pathogen-associated-molecular patterns (PAMPs/MAMPs), damage-associated-molecular patterns (DAMPs), and phytocytokines to induce PRR-triggered immunity (PTI). Certain pathogenic effectors hijack the PTI during infection. Bacterial type III secretion effector HopF2 Pto, a mono-ADP ribosyltransferase, suppresses the PTI via several PTI regulators such as BAK1, MKK5, and RIN4. However, the detailed HopF2 Pto -mediated PTI suppression mechanism still needs to be clarified. To elucidate the regulatory dynamics of the HopF2 Pto -mediated PTI suppression, we conducted RNA-seq analysis after induction of HopF2 Pto under DEX inducible system in Arabidopsis. Intriguingly, transcriptome data shows that the induction of HopF2 Pto reduces the transcript levels of multiple PRRs and PTI regulators such as FLS2, EFR, LORE, CARD1, RDA2, WAK1, and WAK2. Furthermore, HopF2 Pto reduces transcript levels of the precursor of the SCOOP phytocytokines, PROSCOOPs, and their receptor MIK2. In summary, our results provide novel insights into the mechanism by which HopF2 Pto transcriptionally suppresses the expression of PRRs, PTI regulators, and their downstream-signaling genes to desensitize plants to pathogen attack.