VISTA promotes the metabolism and differentiation of myeloid-derived suppressor cells by STAT3 and polyamine-dependent mechanisms
To investigate the function of VISTA in regulating the differentiation of BM-derived MDSCs under normoxia conditions Overall design: BM myeloid progenitor cells were cultured with GM-CSF and IL-6 under normoxia conditions for 4 days. 4 independent biological replicates of  WT vs VISTA  KO BM-derived MDSCs were purified and processed for RNA isolation and  bulk RNA- sequencing analysis.