Homo sapiens Raw sequence reads
High-throughput sequencing of human epithelial cells or B cells.RNA molecules can undergo modification by N-glycans and be displayed on the cell surface. However, recent studies have focused primarily on N-glycan modifications on small RNAs less than 200 nt in length; the transcriptome-wide subtypes of glycosylated RNAs (glycoRNAs) remain poorly characterized. Since glycoRNAs account for only a fraction of the total transcriptome, a validation system for their accurateanalysis has not yet been established. In this study, we aimed to comprehensively characterize transcriptome-wide global glycoRNAs and novel glycoRNA subtypes in both Epstein-Barr virus (EBV)-positive epithelial cells and B cells. Using metabolic labeling and density gradient centrifugation methods, we identified glycoRNAs predominantly below 2,000 nt in both epithelial cells and B cells. Furthermore, we discovered several novel glycosylated long noncoding RNAs ranging from 200 to 400 nt in length. In addition, we discovered a novel EBV encoded transcript, which was named LMP2A-IT1.