RNA-seq of whole ileums from wildtype mice that were either exposed or not with diets with different protein concentrations for 4 weeks
We found that low protein diet consumption for 3 weeks resulted in reduction in the Paneth cells and goblet cells and villous height in wild type mice, indicating that low protein diet could negatively affect gut pathology. We performed transcriptional profiling to identify dysregulated molecular pathways in wild type mice exposed to low diet. Male mice were used at 4-5 weeks of age. The full-thickness ileum of the mice were harvested for transcriptomic analysis after completing 4 weeks of low protein diet treatment.