16S rRNA gene amplicon sequencing of incubated dimictic lake water originating from Grosses Heiliges Meer near Obersteinbeck (Germany)
Investigation of iron cycling including phototrophic Fe(II) oxidation in a dimictic lake, 'Grosses Heiliges Meer' near Obersteinbeck (Germany). Here, the microbial community of lake water from different depths (5m,7m) incubated with Fe, DCMU, or organic matter was analysed over time. Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region, and subsequently library preparation with Nextera 2nd step PCR and sequencing on Illumina MiSeq, v2, 2x250 bp were performed.