scRNA seq of Pdgfra+ cells from the mouse intestine
Sorted PDGFRA+cells  that were lineage-negative for other major intestinal cell type markers (EPCAM1, TER119, CD45, PECAM1) were obtained from the colon of 5 mice. Briefly, mice were given 2.0% (weight/volume) DSS (Thermo Fisher Scientific, #J14489-22) dissolved in water for 7 days for the acute model of DSS. In the chronic model, DSS-administration was followed by a water recovery phase lasting for another 7 days. This cycle was repeated 2 more times, with mice weights being recorded daily. Overall design: 3 Acute DSS treated vs. 2 Chronic DSS treated  samples from Il11mNG reporter mice