Knocking down the eIF4E gene in HaCaT cells, which M5 stimulates to establish the in vitro psoriasis cell model.
We transfected keratinocytes cell line (HaCaT cells) with si-NC and si-eIF4E, and added M5 into the cell culture medium to reveal the function of eIF4E in the in vitro psoriasis cell model. Overall design: We set up si-NC+M5 (n=3) and si-eIF4E+M5 groups (n=3) in HaCaT cells, extracting total RNA after 48 hours, and sent the obtained samples for transcriptome sequencing to reveal the function of eIF4E in psoriatic keratinocytes.