Effect of depletion or overexpression of EGR1 on gene expression in HCC cells (deletion in MHCC97H cells, overexpression in PLC/PRF5 cells)
To investigate the function of EGR1 in HCC growth, we established EGR1 knock out MHCC97H cells by CRISPR/Cas9 system and EGR1-overexpressing PLC/PRF5 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of EGR1 knock out MHCC97H cells and parental MHCC97H cells, EGR1-overexpressing PLC/PRF5 cells and control PLC/PRF5 cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for EGR1 knock out MHCC97H cells and parental MHCC97H cells (EGR1_KO, Mock), EGR1-overexpressing PLC/PRF5 cells and control PLC/PRF5 cells (EGR1, Vector).