Transcriptomic comparison of hepatocytes derived from a panel of human induced pluripotent stem cells with different ethnicities
In the current study, we attempted to establish an in vitro model for assessing population variability in hepatotoxicity testing using a panel of hepatocytes derived from nine human induced pluripotent stem cell (iPSC) lines belonging to three different ethnic groups, Black or African American (BAA), Latino or Hispanic (LOH), and Non-Hispanic White (NHW). The panel of iPSC-derived hepatocytes were exposed to diglycolic acid (DGA) and usnic acid (UA), and transcriptomic changes were characterized and compared. Overall design: Hepatocytes were differentiated from nine iPSC lines belonging to three ethnicities. Cells were exposed to 3.0 mM DGA or 10.0 µM UA for 24 h. Transcriptomic gene expression was characterizecd using RNA-seq. Two replicates (n = 2) were included for each treatment group including the controls.