Dynamic analysis of mononuclear transcription profiles of fat in different parts of mice during aging
Adipose tissue shows significant changes during aging. Here, we used single-nucleus RNA-seq to map the single-nucleus transcription profiles of mouse subcutaneous adipose tissue ( SAT ) and visceral adipose tissue ( VAT ) at a single-nucleus resolution, showing the differences in visceral and subcutaneous adipose tissue changes with aging. The mononuclear sequencing method enabled us to restore all major cell types in mouse white adipose tissue, and we characterized adipocytes, immune cells, and preadipocytes. We demonstrated the existence of different adipocyte subsets and showed that aging leads to a decrease in adipogenic subsets. In addition, with aging, both subcutaneous adipose tissue and visceral adipose tissue showed a decrease in immune mechanisms. Overall design: Subcutaneous mouse adipose tissue depots were dissected from 2 months and 26 months female C57BL/6J mice into ice-cold PBS. diluted in diethyl pyrocarbonate (DEPC) water.White adipose tissue (SAT and VAT) was surgically removed and placed in 1x PBS on ice. Cells were then stained with desired antibodies, and subjected to FACS or snRNA-seq.