Transcriptome analysis of HeLa cells treated with the CDC7-specific inhibitor TAK-931 [RNA-seq]
The results showed that 48-h treatment upregulated 138 genes and 72-h treatment upregulated 504 genes. In the Metascape analyses, the TNF signaling pathway was a dominant network module in the 48-h treatment group, while the inflammatory-related modules were more intensively accumulated, and the signaling networks expanded to the surrounding various modules in the 72-h treatment. Our signaling module results indicate that the TAK-931-induced cellular senescence progressed in a time-dependent manner, and mTOR pathways plays important roles in viability for TAK-931-induced senescent cells. Overall design: Transcriptome analyses were also conducted to capture the signaling networks comprehensively in the TAK-931-induced senescent aneuploid cells. HeLa cells were treated with DMSO or TAK-931 (300 nM) for 24 h, 48 h, or 72 h and then subjected to RNA sequencing (RNA-seq) by NovaSeq 6000.