Exosomal SNHG26 derived from drug-resistant tongue squamous cell carcinoma cells inhibits the anticancer effect of NK cells in the microenvironment
Cisplatin resistance is a common occurrence among patients diagnosed with tongue squamous cell carcinoma (TSCC), resulting in a bleak prognosis. The immune microenvironment, specifically the involvement of Natural killer (NK) cells, plays a significant role in the development of drug-resistant tumor cells and subsequently impacts patient prognosis. However, the precise interaction mode and the underlying mechanisms governing this process remain elusive. Overall design: In this study, a variety of research subjects were utilized, including clinical samples, patient-derived organoids (PDO), TSCC/NK cell co-culture system, patient-derived tumor xenograft (PDX) models, and humanized mice. Various methods were employed, such as drug sensitivity testing, single cell and transcriptome sequencing analysis, gene regulation, DNA/RNA/protein interaction experiments, to investigate and confirm the cascade reaction of TSCC cisplatin resistance-induced NK-related immunosuppression.