CTCF distribution around AsisI sites in IDHwt and IDHR132H DIvA cells
IDH1 mutation impacts CTCF binding and function through methylating cytosine in the CTCF binding motif. We hypothesize that the impairment of CTCF-associated DNA repair process may be relevant to the loss of CTCF chromatin association and shifts in chromatin conformation. Chromatin immunoprecipitation-sequencing confirmed the loss of CTCF coverage in the proximity sites of AsisI sites in IDH1 mutant cells. Overall design: ChIP-seq for CTCF was performed in IDH1 wild-type and mutant DIvA cells with or without 4OHT treatment. 4C-seq with two DSB sites was performed in IDH1 wild-type and mutant DIvA cells with or without 4OHT treatment.