Single cell transcriptome analysis of RUNX3-primed embryonic stem cell derived mesodermal and blood cells
We examined the effects of Runx3 induction in murine embryonic stem cell derived differentiated cells using a doxycycline-inducible transgenic cell line. Embryonic stem cells were differentiated for 8 or 12 days in the presence or absence of transgenic RUNX3. A genome-scale gene expression analysis was employed with single cell RNA sequencing with 10x Genomics technology.