Single-cell transcriptomics of Nr4a3-Tocky T cells in B16 melanoma during immune checkpoint blockade across Tocky progression states
We analyzed CD4+ and CD8+ T cells from tumour-infiltrating lymphocytes (TILs) and tumour-draining lymph nodes (tdLNs) of B16-F1 melanoma-bearing mice treated with anti-PD-L1 and anti-CTLA-4 antibodies or IgG controls. By isolating discrete signalling states (New, Persistent, and Arrested) using flow cytometry, we established a transcriptomic reference for Fluorescent Timer protein kinetics. This dataset enables quantitative mapping of T-cell signalling history onto high-dimensional gene expression and provides a framework for evaluating responses to immune checkpoint blockade (ICB).