RNA-Seq analysis of whole cell lysates, cytoplasmic and nuclear fractions from LENG8 depleted cells and control cells.
RNA-Seq was performed to identify cellular mRNAs that are dependent on the LENG8 protein for their nuclear export. The expression of endogenous LENG8 protein was knocked down by transfection of siRNAs that target LENG8. Control cells were transfected with control siRNAs. RNA-Seq was performed using RNA from whole cell lysates, cytoplasmic and nuclear fractions from LENG8 depleted cells or control cells.