Exploring the Cellular and Molecular Basis of Murine Cardiac Development through Spatiotemporal Transcriptome Sequencing
Spatial transcriptomics is a powerful tool that combines molecular data with spatial information, enabling a deeper understanding of tissue morphology and cellular interactions. In this study, we employed state-of-the-art spatial transcriptome sequencing technology to investigate the development of the mouse heart and establish a comprehensive spatiotemporal cell atlas of early murine cardiac development. Through the analysis of this atlas, we elucidated the spatial organization of cardiac cellular lineages and their interactions during development. Notably, we observed dynamic changes in gene expression within major cell lineages, including fibroblasts and cardiomyocytes. Furthermore, we identified critical genes such as Igf2, H19, and Tcap that may be associated with the loss of regeneration ability during development. Additionally, we predicted potential transcription factors, including Tcf12 and Plagl1, that are likely involved in losing regeneration during the early heart developing stage. Moreover, we successfully identified marker genes, such as Adamts8 and Bmp10 , that can distinguish between the left and right atria. Our study provides novel insights into murine cardiac development and offers a valuable resource for future investigations in the field of heart research.