IKAROS modulates CD19 surface expression and CD19-directed CAR-T response [RNA-seq]
To elucidate how IKAROS modulates CD19 surface expression in B cell acute lymphoblastic leukemic cells, we analyzed gene expression profile from isogenic IKAROS wild type (WT) or knock down (KD) B-ALL cell lines. Overall design: Human B-ALL cell lines (NALM6, REH and SUP-B15) were transduced with IKZF1-specific or scramble shRNA expressing lentivirus. 72 hours post transduction, we compared their gene expression profiles through RNA-seq.