Effect of knocking down PIEZO1 in gastric cancer cells
In gastric cancer (GC), PIEZO1 was suggested to promote cell migration by interacting with Trefoil factor family 1 (TFF1) and serve as a therapeutic target against invasion and metastasis. In addition, PIEZO1 demonstrates abundant expression in most GC cell lines and primary samples and highly-expressed PIEZO1 is associated with poor disease-specific survival. Thus, we try to explore the PIEZO1 function in GC by knocking down assay. Overall design: The potent target genes of PIEZO1 were identified by deep sequencing, using Illumina NovaSeq 6000.