Precise, Specific Gene Editing via a Compact GoCas12m-FokI Chimeric Nuclease
This project contains targeted amplicon deep sequencing data from CRISPR genome editing experiments using a novel compact GoCas12m-FokI chimeric nuclease. Amplicon sequencing was performed on the Illumina MiSeq platform to evaluate on-target editing efficiency and off-target activity at multiple endogenous human genomic loci (CLTA1, HBB, AIFM1, and ABL) and computationally predicted off-target sites. Experiments were conducted in HEK293T, and K562 cell lines. Data includes samples from single-target, multiplex, and AAV-delivered editing experiments with corresponding non-transfected controls.