Saccharomyces cerevisiae strain:Evol-control, Evol-GATE Raw sequence reads
This BioProject contains whole-genome sequencing (WGS) and transcriptome (RNA-seq) data generated to characterize an adaptive evolution strategy for increasing intracellular heme content in *Saccharomyces cerevisiae*. In this strategy, a plasmid carrying a heme-responsive promoter fused to a growth-promoting gene was introduced into a laboratory yeast strain. The resulting positive feedback between intracellular heme levels and cell growth was used as a selection pressure during adaptive evolution. After evolution, the plasmid was removed to obtain a mutant strain with enhanced heme production but without residual foreign DNA.The project includes WGS raw sequence reads for the parental strain and evolved derivatives (including a control evolution line and a line subjected to heme-linked selection). These data are intended to identify genomic mutations associated with increased heme biosynthesis and to confirm the absence of plasmid integration or other unintended foreign sequences. In parallel, RNA-seq raw reads were generated from the parental and evolved strains cultivated under heme-producing conditions. The transcriptome data provide a genome-wide view of changes in gene expression, metabolic pathway reprogramming, and stress responses associated with heme overproduction.Together, these datasets support the genomic and functional characterization of a non-GMO, heme-enriched *S. cerevisiae* strain that may be used as a single-cell protein or heme-rich food ingredient. All raw sequencing data (WGS and RNA-seq) are deposited in the NCBI Sequence Read Archive (SRA) under this BioProject so that other researchers can reproducibly analyze the evolutionary trajectory, mutation spectrum, and transcriptional responses associated with heme-targeted adaptive evolution in yeast.