Amplicon sequencing of replication fork collapse and repair products in Xenopus laevis egg extracts
Illumina amplicon sequencing of a 276 bp synthetic DNA template containing six tandem tetO repeats, amplified from plasmid DNA recovered after in vitro replication in Xenopus laevis egg extracts. Seventeen samples spanning six experimental conditions characterize mutations generated; in the presence or absence of replication (vehicle versus CDC7 inhibition) for collapse at single and converging forks; and in the presence or absence of MRE11 (mock versus MRE11 immunodepletion) for collapse at converging forks.