Transcriptomic profile of monocytes/macrophages from mice with bone, muscle, skin or heart injuries that were treated with systemic delivery of Tregs
Mice with either a cranial bone defect, skin biopsy punch injury, quadriceps volumetric muscle loss defect or myocardial infarction (MI) were treated with Tregs, either via local hydrogel-mediated delivery (for bone, muscle and skin), or with systemic (intravenous) delivery (for heart). Control mice were treated with hydrogel alone (for bone, muscle and skin) or PBS (for heart). On day 4 and 7 post-MI, the injured tissues were harvested and the endogenous monocytes/macrophages were sorted and used for mini-bulk RNA sequencing, to compare the transcriptomic profiles of injured tissue monocytes/macrophages in Treg-treated and untreated mice post-injury. Overall design: Bone (cranial defect), muscle (volumetric muscle loss defect) or skin (full-thickness biopsy punch) injuries were induced in 10 week old male wild-type mice and treated with Tregs locally delivered via a hydrogel directly polymerised at the site of injury. Control mice were treated with hydrogel alone. Heart injury (myocardial infarction, MI) was induced in 10 week old male wild-type mice by left coronary artery ligation. One day post-MI, the mice were administered with either Tregs or PBS (control) via intravenous tail vein injection. Only 1 injury per mouse was performed. On day 4 and 7 post-injury (for all 4 tissues - bone, muscle, skin and heart), the injured tissues from Treg-treated and control mice were harvested, and the monocytes/macrophages accumulating in the injured tissues were sorted by fluorescence activated cell sorting (FACS), then used for mini-bulk RNA sequencing." ***Please note that the Series/Sample records have been updated on July 25, 2024***