Histone modification of melanoma cell lines and cohesin level after knockdown of NIPBL
Histone mark for active enhancer H3K27ac was studied using various melanoma cell lines. SMC1A level on chromatin was studied after knockdown of NIPBL in 501mel Overall design: ChIP-seq was performed using H3K27ac antibody on SKMEL-23, SKMEL-1128, 501mel, COLO800 and A375. SMC1A ChIP-seq was done using 501mel cells with shLuc or shNIPBL