1.Nicotiana benthamiana Transcriptome sequencing  2.Tobacco rattle virus vector delivery of  CRISPR-Cas13d protein for precise gene interference and broad spectrum targeting of Potyvirus
Our data show that TRV-Cas13d mediated greater interference activities against GFP than pEarleyGate100-Cas13d in transient assays. Moreover, TRV-Cas13d also interfered the expression of NbPDS, and made the leaves of the plant white. Meanwhile, the efficiency of interference got lower as the number of sgRNA got more when TRV-Cas13d construct was used to interfere the expression of GFP. In addition, TRV-Cas13d mediated highly robust PVY interference in plant when interfered PVY and eFI4E at the same time