Overexpression of cytidine deaminase genes in human HEK-293T, or mouse C2C12 or NIH-3T3 cells
To identify transcriptome-wide effects of cytidine deaminases, human HEK-293T, or mouse C2C12 or NIH-3T3 cells were transiently transfected with a either an empty-vector control construct or an expression construct for mouse APOBEC3, human APOBEC4, or mouse APOBEC4 cytidine deaminase gene. Comparisons of transcriptome sequences did not reveal any hitherto unknown RNA editing activity of these APOBEC4 enzymes or of mouse APOBEC3 (unpublished work, Santosh Patnaik, Roswell Park Comprehensive Cancer Center, Buffalo, USA).