lncRNA study in TNBC Cell lines
The genetically modified cell lines Hs578T CTR and Hs578T KO LINC01133 were derived from the Hs578T WT cell line. The Hs578T CTR cell line was transfected using a lentiviral system with a closed and empty plasmid vector pL.EF-GFP (AddGene, #57818), serving as a biomolecular control. In contrast, the Hs578T KO LINC01133 cell line was transfected via a lentiviral system with a plasmids vectors pL-CRISPR.EF.GFP and p-lentiCRISPRV2 (AddGene, #57818 and #52961) containing gRNA sets and a CRISPR/Cas9 sequence for knockout of the LINC01133 gene.