p53-dependent transcription in hTERT-RPE1 cells
We investigated the genes that were most strongly activated by p53 in this immortalized human cell line following treatment with the MDM2 inhibitor nutlin-3a. To identify genes that were activated by p53, we comparatively analyzed parental cells and isogenic derivatives. Wild type, p53-knockout and cells harboring a heterozygous p53 A276P mutation were assessed. Overall design: Comparative gene expression profiling analysis of RNA-seq data for hTERT-RPE1 cells and its derivatives (p53-/- and p53+/A276P).