Effect of TUC-seq protocol versus SLAM-seq on quantification bias in nucleotide conversion RNA-seq data
To investigate the effect of TUC-seq versus SLAM-seq on quantification bias in nucleotide conversion RNA-seq, we performed SLAM-seq and TUC-seq on NIH cells. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples. Overall design: Control sample, Uridine treatment (no conversion), 4sU treatment (no conversion), SLAM-seq and TUC-seq on NIH cells in two replicates.