m6A sequences 2 WT_input vs 2 Ythdf1 KO_input
Methylated RNA immunoprecipitation(MeRIP) with specific m6A antibody and used for library construction and the next generation sequencing, to identify m6A modified transcripts in WT or Ythdf1 deficiency colon epithelial cells after DSS treatment Overall design: 2 WT_input vs 2 Ythdf1 KO_input