Mycobacterium tuberculosis Genome sequencing
The application of pan-genomics in understanding Mycobacterium tuberculosis complex (MTBC) transmission remains understudied, particularly in high-burden settings such as China. We constructed an MTBC pan-genome (MTB_pan) using 307 complete genomes representing all eight lineages. Core and accessory genomes were analyzed, lineage-associated genes were identified, and functional annotations were assessed using Pfam domains. Transmission dynamics were evaluated by comparing pan-genome- and H37Rv-based approaches for isolates collected from China, focusing on alignment rates, clustering efficiency, and single-nucleotide polymorphism (SNP) distances.