Bulk RNA-seq of multi-tissue transcriptomes from control and DHEA-treated mice (ovary, uterus, thymus, brain, heart, intestine, kidney, liver and spleen)
To characterize systemic transcriptional changes induced by DHEA treatment, we performed bulk RNA-seq across a panel of tissues collected from control and DHEA-treated mice. Total RNA was extracted from each tissue using a kit-based workflow following mechanical homogenization in lysis buffer. RNA concentration/quality were assessed prior to library construction and high-throughput sequencing. Overall design: Control and DHEA-treated mice were included. For each group, tissues were collected for transcriptomic profiling, including ovary, uterus, thymus, brain, heart, intestine, kidney, liver and spleen. Immediately after dissection, tissues were placed into lysis buffer, homogenized using a tissue homogenizer, and total RNA was purified using a commercial RNA extraction kit. Purified RNA was quantified and quality-checked, then submitted for bulk RNA-seq library preparation and sequencing.