RNASeq of Vitis vinifera cv. Chardonnay silenced for VvPLL3
This work is detailed in the manuscript, Down-regulation of VvPLL3 enhances grapevine resistance to Erysiphe necator via defense activation and cell wall remodeling, submitted to the journal Plant Physiology. Artificial microRNA mediated down-regulation of the VvPLL3 gene in Vitis vinifera cv. Chardonnay resulted in pleiotropic effects in one, but not all, of the silenced lines showed a consistent reduction in colony success, ranging from 11 percent to 26 percent. The reduction in colony success was mainly associated with a 34-59 percent decrease in tertiary hyphal branching at 48 hours post-inoculation. These samples were collected to characterize global gene expression in the silenced and control lines.