Mouse bronchoalveolar lavage fluid metagenomes with fecal DNA contamination spike-in
Low-biomass respiratory microbiome samples are highly susceptible to exogenous DNA contamination, which can bias shotgun metagenomic profiling. This BioProject provides whole-genome shotgun metagenomic sequencing data from bronchoalveolar lavage fluid (BALF) collected from eight individual mice (Mus musculus). To experimentally model contamination, fecal DNA extracted from a single donor mouse was spiked into each BALF DNA sample prior to library preparation. The dataset is intended for evaluating and benchmarking contaminant detection/decontamination strategie in low-biomass metagenomes. Each sequencing run corresponds to one mouse BALF sample processed with the fecal DNA spike-in, with accompanying sample metadata.