Establishing murine trophoblast organoids as a platform for CRISPR-Cas9 screening [sgRNA]
Murine trophoblast organoids contain trophoblast cell types. We used CRISPR SCREENING to analyze the trophoblast organoids derived from TS (mTOs). Overall design: Trophoblast organoids in the maintenance condition and the differentiation condition were digested into single cells by accutase and papain, respectively.  NGS analysis.