Transcriptomic Analysis of Air–Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation
We comprehensively analyzed differentiation effects of the air–liquid interface (ALI) culture method on adult tissue-derived human lung organoids and examined the similarity with in vivo human airway epithelium compared to submerged cultures using single-cell RNA sequencing. Overall design: 8 samples of epithelial cells differentiated from normal lung organoids derived from 2 patients were dissociated and analyzed using scRNAseq.