Identification of a new HLA-C*16 allele Iin a french  voluntary bone marrow donor
Here we describe a novel C*16 allele, identified in  a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology. Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:  A*29:02, *68:01 B*44:0244:03 C*07:04, *16 :New DRB1*07:01, *10:01 HLA-DRB4*01:01 DQA1*01:05, *02:01 DPA1*02:01,*- DPB1*11:01, *13:01 HLA-C*16 : 01 allele differs from C*16:01 :01 :01 by one nucleotide substitution at position 235 of exon 3 (position 578 of cDNA), where  base G is replaced by A , inducing Arg instead  His (169CGS>CAC).