A study on gene expression responses in the common carp to cooling stress using RNA-seq.
Common carp (~0.5kg) previously held for over 3 months at 25°C were randomly placed into two large aquarium tanks, one which remained throughout at 25°C (control) and the other that was cooled over 3 days to 11°C and held for 23 days.  Replicate specimens from both tanks were then killed at up to 17 timepoints after cooling, kidney tissue was sampled, and transcript expression as a function of time after cooling determined by RNA-seq.