Single-cell RNA sequencing (scRNA-seq) profiles of fetal human cortical neurons
The dataset consists of single-cell RNA sequencing (scRNA-seq) profiles of fetal human cortical neurons (10 weeks gestation) under six experimental conditions: Sample 1 – Control: Untreated cells. Sample 2 – PFF treated: Cells exposed to alpha-synuclein pre-formed fibrils (PFF) for 14 days. Sample 3 – PFF + B36D: Cells treated with PFF and the peptide inhibitor B36D. Sample 4 – B36D alone: Cells treated with B36D without PFF. Sample 5 – PFF + S62: Cells treated with PFF and the peptide inhibitor S62. Sample 6 – S62 alone: Cells treated with S62 only. Culture and treatment timeline: All cells were initially cultured for 7 days before treatment. Treatments were applied for 14 days. On day 21 in vitro, cells were dissociated for single-cell RNA sequencing. This dataset captures the gene expression changes associated with PFF-induced pathology and the potential rescue effects of peptide inhibitors B36D and S62 in human cortical neurons. It allows comparisons between control, disease-mimicking, and treatment conditions at single-cell resolution.