Toxin Repertoire of the Deep-Sea Zoantharian Zibrowius primnoidus (Cnidaria: Zoantharia)
This BioProject presents the transcriptomic analysis of the deep-sea zoantharian Zibrowius primnoidus, collected at 255 m depth in the Sicily Channel, Italy, to characterize its toxin repertoire using a bulk RNA-seq approach. Twelve polyps were preserved in RNA later, washed in PBS, and processed using a TRIzol-based RNA extraction with magnetic beads. After purification (Mag-Bind Total Pure NGS kit), RNA integrity was assessed via TapeStation (High Sensitivity RNA Screen Tape Assay). Library preparation used the Zymo-Seq RiboFree Total RNA Kit for rRNA depletion, followed by paired-end 150 bp sequencing on an Illumina NovaSeq 6000, generating 90,741,564 raw reads. Primary bioinformatics included base calling, demultiplexing, and adapter masking with Illumina BCL Convert v3.9.31. This dataset provides insights into the molecular adaptations and venom composition of Zibrowius primnoidus, contributing to the study of deep-sea cnidarian toxins.