Analysis of LPS-induced gene expression of OTULIN-deficient murine macrophages by RNA sequencing
In order to unravel the role of the linear deubiquitinase OTULIN in LPS-induced gene expression in macrophages, we performed RNA-sequencing on primary bone marrow-derived macrophages of mice lacking OTULIN selectively in myeloid cells. Myeloid cell-specific OTULIN knock-out mice develop an auto-inflammatory phenotype and macrophages derived from these mice display increased LPS-induced inflammasome activation and cell death. Through analysing LPS-induced gene expression analyses on OTULIN-deficient macrophages we aimed to identify signalling pathways and genes that regulate inflammasome activation and cell death in these cells, in order to get new insights on the molecular events that regulate the development of autoinflammation in myeloid cell specific OTULIN-deficient mice. Overall design: RNA was sequenced from BMDMs from Otulin floxed mice (WT) and from Otulin floxed mice carrying a Cre recombinase under the LysM-promotor (KO) at 0, 3, 6, 12 and 24 hrs post LPS treatment. Four biological replicates were included per genotype.