Dual_guide_CRISPR_Cas9_library_to_identify_novel_synthetic_lethal_gene_pairs
The aim of the study is to identify novel synthetically lethal gene pairs within cancer cells that could potentially be therapeutically targeted in the future. To achieve this, we designed a bespoke dual guide CRISPR library targeting our gene pairs of interest and used this library to screen a panel of cell lines from a background of melanoma, NSCLC and pancreatic cancer.