Leaf transcriptome sequencing of several wild barley accessions
RNAseq was performed on several wild barley accessions to understand the gene content at Mla (Mildew locus a). Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters.