Root-associated fungal community of oat (Avena sativa) (QVIDJA organic soil amendment experiment)
In this two-year study we tested how four different organic soil amendments affected root-associated fungal communities of oat (Avena sativa). The study consisted of three replicates of each following treatments: fertilized control (80N), composted wood fiber (CWF), lime-stabilized wood fiber (LWF), spruce biochar (SB) and willow biochar (WB). All the treatments were fertilized similarly with 80N treatment (80 kg of N / ha). Additionally, similar samples were collected from the plots, where humus water treated oat seeds were grown and from unfertilized (0N) plots, but sequence data from these samples were not analyzed with other samples. The experiment was set up in August 2016, when organic soil amendments were applied to the field. Three oat samples per each treatment plot were collected at each sampling time (June, July, and August 2018). Root samples were frozen with surrounding soil and cleaned later with tap water. After cleaning, roots were freeze-dried and pulverized. Root-associated fungal community composition was characterized using high-throughput sequencing with two primer pairs (fITS7 and ITS4, and WANDA and AML2) of rDNA region. Each sample were characterized separately in the case of WANDA-AML2, whereas in the case ITS2 region, three samples were combined within a plot after DNA extractions.