Germline inheritance of H3K9me3 and its function in constitutive heterochromatin establishment.[CUT&Tag]
To investigate the function of H3K9me3 in constitutive heterochromatin formation in early embryo we have created embryos that are devoid of H3K9me3 Overall design: different stages of embryos of control, single mutants and triple mutant were subjeted to Cut and tag for H3K9me2/3,  HP1a, and H3.