scRNA-seq of mouse periodontal ligament labeled with LepR-cre; R26-tdTomato; Runx2-GFP
We employed a cell sequencing approach using 10x Genomics scRNA-seq to study the genetic profiling of LepR+ cells in the periodontal ligament (PDL). It has been suggested that PDL-derived LepR+ cells maintain hard tissue homeostasis by differentiating into hard tissue-forming cells such as osteoblasts and cementoblasts. In this study, we generated LepR-cre; R26-tdTomato; Runx2-GFP mice, and performed scRNAseq using their PDL cells. This study is the first genetic profiling of a LepR-cre-labeled population in PDL. Overall design: PDL cells of LepR-cre; R26-tdTomato; Runx2-GFP mice were collected by Fluorescence-activated cell sorting (FACS) and analyzed using scRNA-seq