Differential Notch target genes expression profile upon GSI washout.
The Notch signaling pathway regulates several processes including development and differentiation and its aberrant regulation leads to several diseases. Here, we analyzed the gene expression profile upon washout of the Notch inhibitor DAPT in mouse pre-T (Beko) cells. Overall design: Mouse pre-T cells (Beko) were treated with gamma-secretase inhibitor (GSI) DAPT for 48 hours. After washing out the inhibitor, cells were placed back in culture for 24 hours before analyzing the gene expression profile by RNA-seq.