Repressive chromatin regions of Osimertinib-treated PC9 cells with H3K27me3 or H3K9me3 modifications through H3K27me3 ChIP-seq or H3K9me3 ChIP-seq
Drug tolorent persister cells induced by target therapy are marked by elevated level of compressed chromatin structure. We use ChIP seq against H3K27me3 or H3K9me3 antibody to reveal the repressive chromatin regions of osimertinib-treated PC9 cells. Overall design: PC9 cells transfected with non-target sgRNAs were treated with 150nM Osimertinib for 4 days to generate the drug tolorent persister cells. Cell samples were collected and undergo ChIP seq using H3K27me3 or H3K9me3 antibody respectively. *************************************************************** Raw files for human/patient samples are available in dbGaP (https://www.ncbi.nlm.nih.gov/gap/) phs004458.v1.p1 for controlled access to the personally identifiable sequence data. ***************************************************************