Single-cell RNA sequencing of PBMC from Scleroderma patients
We performed single-cell RNA sequencing of 63 cryopreserved PBMC samples from SSc and SSc-ILD patients in 6 batches, with 2 batches processed using a Chromium Next GEM Single Cell 3' Kit (10X Genomics) and 4 batches processed using a Chromium Next GEM Single Cell 5' Kit (10X Genomics) for data generation. Whole genome sequencing was performed for genotyping/demultiplexing.