Early/late Repli-seq for measurement of replication timing in mouse Hep-74.3a cells
Replication time profile of mouse hepatoma derived Hep-74.3a cell line. Two ENCODE-style biological replicates where cells were separately grown, labelled with BrdU and FAC sorted into early and late S-phase populations. Sorted cells were then subject to the E/L Repli-seq protocol Illumina sequencing library preparation and sequenced on an Illumina NextSeq550.