A novel CRISPR/Cas9 screening platform identifies an IRF1-SOCS1-mediated negative feedback loop that limits CXCL9 expression and anti-tumor immunity (Human RNA-Seq)
Investigation and validation of genes that regulate CXCL9 expression by RNA sequencing Overall design: Human monocytes were gene-edited and then derived into macrophages using M-CSF. Macrophages were stimulated with 1ng/mL IFNg for 17 hours.