Thymocyte mRNA sequence from Eed conditional knockout mice
Histone H3 lysine 27 tri-methylation (H3K27me3) is associated with transcriptional repression by the enzymatic activity of Polycomb Repressive Complex 2 (PRC2), which consists of three core subunits, Eed, Suz12 and Ezh1/Ezh2, and maintains specific gene expression patterns during development. Eed was found to be required not only for initial activation but also for the amplification of PRC2 activity for H3K27me3. Therefore, to understand the underlying epigenetic mechanisms directing cells from DP thymocytes, we generated mice conditionally lacking Eed from the DP thymocyte stage. DP thymocytes were sorted from 3 pairs each of control or Eed cKO mice and RNA-seq was performed.