Single cell RNA sequencing of Nasopharyngeal Carcinoma Cell lines infected with Epstein-Barr virus (EBV) subjected to air-liquid interface (ALI)-induced reactivation
The HK1-EBV cell line is a human nasopharyngeal carcinoma cell line (RRID:CVCL_7084) infected in vitro with recombinant Epstein-Barr virus (EBV) Akata strain expressing EGFP and neoR inserted into the BXLF1 locus (PMIDs: 16611410). This cell line has been authenticated to be free of HeLa contaminants (PMIDs: 18196576; 24991015). We used bulk RNA sequencing techniques to analyze these cell lines reactivated at the air liquid interface (ALI) in a 3D cell culture model for ALI-induced EBV reactivation. Overall design: Cells were cultured at the air-liquid interface (ALI) to induce EBV reactivation. N-terminally tagged LMP1 China 1 strain (HA Ch1) and IRES-EGFP (control) expressed from pBabe-puro vectors were stably introduced by retrovirus transduction. ALI cultures were collected on Day 0,2, and 4 post-lifting.