RNAseq of iPSC derived pulmonary organoids by co-culturing endodermal and mesodermal progenitors
The mesoderm is important in driving pulmonary organogenesis. We described a new method for rapid producing pulmonary organoids from human induced pluripotent stem cells (iPSCs) by co-culturing anterior foregut endoderm (AFE) and mesoderm progenitors. The pulmonary organoids spontaneously formed within 14 days of the co-culturing. We measured gene expression on the pulmonary organoids and normal human iPSCs using RNAseq.