16S rRNA gene sequencing from CLP and Sham model mice
Sepsis is a prevalent and life-threatening critical condition in clinical practice. The pathogenesis of sepsis is complex, and it carries a high mortality rate. Recently, the regulatory role of the gut microbiota in the pathophysiology of sepsis has garnered increasing attention. To investigate this role in depth, we established a murine model of sepsis induced by cecal ligation and puncture (CLP). Fecal samples were collected and subjected to high-throughput 16S rDNA sequencing analysis. Our findings demonstrate that the gut commensal bacterium, Limosilactobacillus reuteri, and its metabolite, indole-3-carboxaldehyde (IAld), play a crucial beneficial role in the progression of sepsis. This study provides novel experimental evidence for elucidating the pathogenesis of sepsis and suggests that targeting the gut microbiota may represent a potential therapeutic strategy for this condition.