Spatial transcriptomics of 3-month-old mouse ovaries
We used Visium technology (10X Genomics) to infer cell-to-cell communication in ovaries based on spatial proximity. Ovaries from a 3-month old mouse in diestrus were collected and frozen in OCT. 10 µm thick tissue slices were placed on Visium Spatial Gene Expression Slides (10X Genomics) and stained with Hematoxylin and Eosin (H&E). Libraries were prepared by manufacturer's recommendations and sequenced on NovaSeq6000.