Nuclear translocation of PTH receptor hijacks thyroid hormone signaling for bone mass regulation [ChIP-Seq]
After preliminary verification of physical interaction between PTH1R and THR, we next performed chromatin co-immunoprecipitation sequencing (ChIP -seq) to detect the overlapping downstream promoters genome-widely between PTH1R and THR. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for PTH1R and THR in MC3T3-E1 cells.