air bacterial communities in building with expansive basement
This study analyzed airborne bacteria communities in expansive underground buildings via high-throughput sequencing of 16srDNA V4 fragment. In this study, we employ a meticulous approach to collect biological microbe samples using cotton swabs that have been pre-immersed in the 1-ml Liquid Amies preservation medium of Eswabs to ensure the maximum capture of biomass. The sampling process, conducted from September 2023 to August 2024 across 44 distinct sites and a total of 452 DNA samples and 36 RNA samples within the science buildings at XJTLU, includes a variety of environmental surfaces, such as vents.