Comparative bulk RNAseq analysis of Control and Ptch1-deficient PTHrP+ dental follicle cells.
We set out to reveal the transcriptome change induced by Ptch1 deficiency in PTHrP+ dental follicle cells Overall design: tdTomato+ cells were FACS-isoalted from postnatal day 8 (P8) dental follicles of Ptch1 conditional Het and conditional KO mandibular molars (tamoxifen pulse at P3). mRNA were amplifed by SMARTer mRNA-seq kit (Clonetech/Takara) before deep-sequenced by Illumina HiSeq4000.