Time-series spatial transcriptomic analysis of murine small intestine (SI) undergoing irradiation induced injury-repair with and without LXR activation
To unbiasedly characterize the spatial transcriptomics landscape of murine small intestine damage and repair, we performed a time-series spatial transcriptional analysis of total small intestinal tissue during the course of irradiation damage from mice receiving standard diet. Additionally, to understand the role of LXR activation during damage and repair, we also collected small intestine tissues from mice fed with GW3965 diet. Overall design: Wild type C57BL/6J male mice were fed with either a control (standard) diet or a diet with LXR agonist GW3965. Ten days after diet, mice were exposed to 10Gy total body irradiation (TBI) and SI Swiss rolls (starting from distal SI) were collected on day3 after irradiation (3 dpi), processed and submitted for sequencing. SI Swiss rolls from mice fed with either standard or GW3965 diet but no irradiation (0 dpi) were used as control.