Single-cell RNA sequencing analysis of humanized liver
Single cell RNA sequencing of liver cells enriched for Non-Parenchymal cells (NPCs) to see the percentage of humanizaton, the gene signature and the heterogeneity of most types of human NPCs in a humanized liver. Overall design: Single cell RNA sequencing of the liver cells enriched in Non-Parenchymal cells (NPCs). Liver cells were isolated from median and left lobe of the mouse after enzymatic treatment with Collagenase II and DNAase. The vast majority of hepatocytes were removed by centrifugation in low spped  (80g).  Single cell RNA sequencing was performed with .......