P14 T cells from C57BL6J draining lymph node after vaccinia-GP33
CD90.1 T cells were isolated from draining lymph nodes of vaccinia-GP33 infected ears 45 days post infection Overall design: P14 T cells from draining lymph nodes were MACS enriched for CD90.1, then flow sorted on CD90.1+, CD8+, CD44+, Live (DAPI-), CD45 IV- (extravascular). Enriched cells were then submitted to NYU genomics center for single cell RNA sequencing