Novel species Tolumonas sp. WCU1
A stream water sample was collected from Dicks Creek on the campus of Western Carolina University, Cullowhee, North Carolina, USA on 22 August 2024. It was stored for one week at 4?C then spread-plated onto an R2A media plate. A small white colony from the R2A plate was selected for isolation after a week of incubation at room temperature (~25?C). The colony was streaked onto fresh R2A media until a pure culture was obtained. Sanger sequencing of its 16S rRNA identified it as belonging to the genus Tolumonas. No exact species match was obtained from GenBank and it matched poorly (<96%) to two species in the genus, including T. osonensis (NR 108829.1) and T. auensis (NR 074805.1). The isolate was sent to Plasmidsaurus, Inc. to have its whole genome sequenced using a hybrid approach of Oxford Nanopore Technology and Illumina Miniseq methods and data generated from that project are included here.