Effect of lactic acid bacterium 2004 on transcription profile in wildtype animals and daf-16 mutants.
Examine the effect of lactic bacterium 2004 supplementation on expression profile in wld type animals. Overall design: Total RNA was isolated using TRIzol reagent (Invitrogen) from C. elegans Bristol strain N2 cultured in S basal medium with E. coli OP50 or L. paracasei subsp. paracasei 2004 for three days from three days after hatching. RNA-seq library preparation and RNA sequencing were performed by Macrogen Inc.