Transcriptomic analysis of triterpenoid biosynthesis in Inonotus obliquus under rice solid-state cultivation
Triterpenoids are important bioactive compounds in Inonotus obliquus, with inotodiol being one of the most recognized for its diverse biological activities, including antitumor effects and potential applications in cancer treatment. However, its biosynthetic pathway remains unclear. In this study, we employed a rice based medium solid-state fermentation strategy to cultivate I. obliquus and obtained inotodiol and lanosterol with a yield of 10 mg/kg and 100 mg/kg. To investigate the genes involved in triterpenoid biosynthesis, we conducted transcriptomic analysis on mycelia collected at different cultivation time points. The expression levels of triterpenoid related genes exhibited an increasing trend, which was further validated by qPCR. Additionally, a comparative analysis of post modification genes identified several potential cytochrome P450 enzymes involved in triterpenoid biosynthesis.