Cell lines treated with BrdU against DMSO controls (DNA damage-induced senescence)
Cells were treated chronically for 7 days with a sub-lethal dose (100uM) of 5-Bromodeoxyuridine (BrdU) to trigger DNA damage-induced cellular senescence. Senescence phenotype was confirmed with multiple cellular markers such as SA-b-Gal staining, lamin B1 downregulation, increased size of nucleus, metabolic shift, etc. Total RNA was isolated after day 7 of treatment (3 samples DMSO, 3 samples BrdU) and RNA-seq was performed. Cell lines used: HBEC5i: endothelial cells, HMC3: microglia, HOG: Oligodendrocytes, SKNMC: neuronal cells, SVGA: astrocytes