RNA-seq of Arabidopsis thaliana in cold condition by why1
This project focuses on the molecular mechanism of RAF3/6 in regulating plant cold stress responses. Under low-temperature stress, RAF3/6 activates nuclear cold-responsive pathways through the transcription factor WHY1. Chloroplast photosynthetic disturbance modulates nuclear gene expression via retrograde signaling. To investigate the effect of photosynthesis inhibition on nuclear gene expression, RNA-seq was performed in wild-type, raf3-1, and raf6-1 mutant plants under control and cold treatment conditions. Thousands of differentially expressed genes were identified, and functional enrichment analysis showed that these genes are mainly involved in cold response, stress adaptation, and signal transduction. This study aims to reveal the regulatory module of RAF3/6-WHY1 in retrograde signaling and cold stress responses, providing a theoretical basis for understanding plant stress tolerance and molecular breeding.