Spatial analysis of the effects of L-DOPA on gene expression in the prefrontal cortex in a mouse model of Parkinson's disease
The goal of the assess the effects of L-DOPA on gene expression ingene expression in the coronal section of the mouse brain and model with progressive loss of dopaminergic neurons, the TIFI-A(DATCreERT2) strain. Induction of the mutation with tamoxifen causes progressive loss of neurons expressing the dopamine transporter in the ventral midbrain. Mutation was induced by tamoxifen treatment at the age of 12-14 weeks, and 14 weeks later animals received a 14-days treatment of i.p. injection of L-DOPA at 18 mg/kg. Mice were killed 1 hour after the last injection, coronal sections of the brain 1,18-1,98 mm from bregma were mounted on Visium slides, stained and processed for construction of sequencing libraries.