Rattus norvegicus kidney transcriptome after renal ischemia-reperfusion injury and rapamycin treatment
This project aims to investigate the protective mechanism of rapamycin against renal ischemia-reperfusion injury (IRI) using RNA-seq transcriptomic analysis. We used male Sprague-Dawley rats to establish a renal IRI model (45 min ischemia followed by 3 or 7 days of reperfusion), with sham-operated rats as controls. Rapamycin (1 mg/kg/d or 2 mg/kg/d) was administered by gavage for 3 or 7 consecutive days to evaluate its protective effect. Renal cortex tissues were collected from all groups for total RNA extraction. Sequencing was performed on the Illumina NovaSeq 6000 platform, generating paired-end 150 bp reads. The raw sequencing data will be used to identify differentially expressed genes, explore the regulatory role of rapamycin on autophagy and inflammation pathways, and investigate the involvement of COL6A6 and related signaling axes in renal IRI, providing potential therapeutic targets for clinical renal IRI.