Four Hi-C datasets of whole zebrafish embryos at 24 and 48 hours post fertilization
In this experiment, we've examined chromatin conformation of zebrafish embryos at 24 and 48 hours post fertilization (hpf). Fish were reared as described in (Westerfield et al., 2009). We performed a modified in situ Hi-C protocol from (Rao et al., 2014) and that can be found in detail at (Díaz et al., 2017, under revision). Samples were digested with MboI restriction enzyme having as starting material 10 embryos with two biological replicates per sampling point. The aim of the experiment was to characterize the 3D structure of zebrafish chromatin and compare it with another vertebrate (H. sapiens and M. musculus) and invertebrate datasets (D. melanogaster and C. elegans).