RNA sequencing of U937 cell clones carrying 0,1 or 2 copies of FURIN gene
Pro-monocytic U937 cells were subjected to FURIN gene editing via an optimized CRISPR technology, resulting in clones with 1 or both copies of FURIN deleted. Paired-end RNA sequencing was performed on these cells, in addition to wild-type cells, to determine the effects of FURIN depletion on the transcriptome. Overall design: 3 cell-types (WT, hemizygous (1 copy of FURIN present), and nullizygous (0 copies of FURIN present) x 3 replicates, subjected to paired-end RNA sequencing.