Restriction-site associated DNA sequencing of the Oh-Shamo and White Plymouth Rock breeds and their F1 and F2 chickens
Eighteen F1 chickens were obtained by crossing a male of the Oh-Shamo breed with four females of the White Plymouth Rock breed. These F1 chickens were crossed to obtain 279 F2 chickens. Genomic DNA extracted from the blood cells of all individuals was subjected to restriction-site associated DNA sequencing using two restriction enzymes, EcoRI and MspI, to create a high-density SNP linkage map of the F2 population.