Bacteria isolated from Antarctic ice-free soils (Psychrobacillus sp. L3, Psychrobacillus sp. L4, Sporosarcina sp. A2) as well as their bacteriophages Spoks, Perkons, and Lietuvens. Whole genome sequencing and assembly.
Sequencing data relevant to the project "Structural and functional studies of proteome encoded by three novel temperate DNA bacteriophages isolated from Antarctic soil" (lzp-2022/1-0111). The sequencing data generated and analyzed within the scope of the project is expected to expand the current knowledge on the diversity of culturable bacteria from Antarctic soils and their phages.Previously, we retrieved three novel temperate bacteriophages and their host bacteria from the Antarctic soil samples collected during the first Latvian Antarctic Expedition. The current project mainly focuses on the elucidation of functions for evolutionarily conserved proteins of unknown function found in the proteome of the Psychrobacillus phage Perkons, Psychrobacillus phage Spoks, and Sporosarcina phage Lietuvens, which are the first cultured phages known to infect the representatives of the aforementioned bacterial genera. The novelty of phages made it interesting to elucidate the genomes of their hosts to enable phage-host interaction studies at a finer scale, as well as to expand the knowledge on the culturable Antarctic bacteria genomic diversity.Within the project, we have planned to perform the RNA sequencing of the phage+host systems at different time points after the infection to temporally resolve the transcriptome of the phages and see how the host responds to the infection. For this, we needed to obtain not only the complete annotated phage genomes (which were already publicly released by us through GenBank several years ago) but also sequence, assemble, and annotate the complete genomes of the host strains for the aforementioned phages. During the course of the project, we will release complete annotated genomes of the aforementioned novel bacterial isolates alongside the relevant Illumina (250 bp PE) and long-read (ONT) datasets that allowed us to assemble these complete genomes that will be annotated using PGAP.This BioProject will be used to make all the relevant sequencing data and assemblies related to this project publicly available without any restriction to reinforce the results and conclusions drawn from the sequencing part of the research performed within the lzp-2022/1-0111 project.