Transcriptome regulated by mitochondrial uncouplers in prostate cancer cells
We discovered that mitochondrial uncouplers represented by nitazoxanide and its active metabolite tizoxanide inhibited prostate cancer growth. This RNA-seq study aims to elucidate the mechanism by which mitochondrial uncouplers inhibit prostate cancer growth. The results indicate that mitochondrial uncouplers predominantly downregulated E2F1 target genes and thus likely inhibited prostate cancer growth through impeding E2F1-mediated transcription. Overall design: The prostate cancer cell line C4-2 was treated with DMSO or 20 uM of tizoxanide (TIZ) for 16 h. Total RNAs were prepared from the cells and subjected to RNA-seq. C42_DMSO_1, C42_DMSO_2, and C42_DMSO_3 are the samples treated with DMSO, while C42_Tiz_1, C42_Tiz_2, and C42_Tiz_3 are the samples treated with TIZ.