RNA-sequencing of mice 24 hours following acute p53 activation due to Mdm2-loss (Mdm2FM/- Cag-CreERTg (FMTg)) compared to mice that retain Mdm2 (Mdm2+/- Cag-CreERTg (Tg))
FMTg (Mdm2FM/- Cag-CreERTg) and Tg (Mdm2+/- Cag-CreERTg) mice were administered 6mg/40g of Tamoxifen dissolved in corn-oil and sacrificed 24 hours after injection. Tissues were flash frozen and RNA was isolated using TriZOL reagent as per manufacturers protocols. The goal of this study is to understand the differences and similarities between the p53 transcriptome in various murine tissues Overall design: 5 Biological replicates of FMTg (Mdm2FM/- Cag-CreERTg) Pancreas, Kidney, Heart, Ovary, and Small Intestine and 3 biological replicates of Tg (Mdm2+/- Cag-CreERTg) Pancreas, Kidney, Heart, Ovary, and Small Intestine were submitted for RNA-sequencing