Flora analysis (methanogenic fermenters fed rumen fluid-treated rapeseed )
A pretreatment of lignocellulosic substrates with cattle rumen fluid was successfully developed to improve methane production (https://doi.org/10.1016/j.jbiosc.2016.11.008 ). In the current study, a 16S rRNA gene-targeted amplicon sequencing approach using the MiSeq platform was applied to elucidate the effect of rumen fluid treatment on the microbial community structure in laboratory-scale batch methane fermenters.