Capturing totipotency in human cells through spliceosomal repression [ChIP-seq]
Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for h3k4me3 and h3k27me3 in hESC sand hTBLCs Overall design: Comparative epigenetic profiling analysis of h3k4me3 and h3k27me3 data for hESCs and hTBLCs