Genome-wide mapping of NRF1 (NFE2L1) binding in HCT116 cells upon proteasome inhibition.
The maintenance of protein homeostasis is an essential characteristic of life. Transcription factor NRF1 (NFE2L1) has been reported to be activated by proteasome dysfunction, although the genome-wide target genes are poorly understood. Using ChIP-seq analysis, we found a potential association between NRF1 and autophagy. Our findings highlight the new activation mechanism of autophagy through gene regulation under proteasome dysfunction. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for transcription factor NRF1 in HCT116 cells treated with 1 µM MG132 for 16 h.