Evaluation of the miRNA Expression Profile in Bovine Mammary Glands Artificially Infected as a Tool for Identifying Candidate Genes for Mastitis Resistance.
The objective of this project is to evaluate the set of microRNAs (miRNAs) that are differentially expressed following challenge with Streptococcus agalactiae (experimental infection model). This goal will be achieved using RNA-Seq methodology combined with gene network analysis. Based on these results, gene networks associated with the inflammatory response will be constructed, and the miRNAs that may serve as candidate resistance genes will be identified. Identifying genes expressed in response to mastitis, as well as understanding their regulatory interactions, will contribute to a better understanding of the pathophysiology of the disease. These findings may be used to support the development of more effective treatments and to identify markers associated with resistance to the pathogen.