16S rRNA sequencing of human fecal samples during a resistant starch dietary intervention study
16S rRNA amplicon sequencing of human fecal samples before and during a dietary intervention study to predict gut microbial response to resistant starch consumption. We amplified the V4 region of the 16S rRNA gene using 515F and barcoded 806R primers and performed MiSeq 2x250bp paired end sequencing.