scRNAseq of Cd45+ cells from brains of ApoE-variant Alzheimer's mice sorted by ex-vivo amyloid beta uptake
Here we perform single cell sequencing of immune cells of aged AD mice bearing knock-in of human APOE variants in the murine APOE locus, sorted into high-uptake and low-uptake pools after incubation with fluorescently labeled amyloid beta. Overall design: We produced single cell suspensions from the hippocampal and cortical regions of 60-week-old 5XFAD-hemizygous mice bearing homozygous knock-in of APOE2, APOE3, or APOE4 in the murine Apoe allele. Cells were incubated with fluorescently labeled amyloid beta before being sorted for Cd45+ and split into a high- and low-uptake pool. Cells were hashed post-sort and sequenced as a single library by 10X 5'v2 single cell sequencing.