Amplicon sequencing of phytoplankton assemblages in a sediment core in Onagawa Bay
The aim of this study is to use NGS to analyze the long-term change of the phytoplankton assemblages in a sediment core. A sediment core was collected in Onagawa Bay, Japan. After DNA was extracted from all samples, the PsbA gene were amplified with primers (PsbAyo1F-PsbAyo1R) using PCR. The PCR product mixtures were analyzed for pair-end DNA sequencing of 300 bp × 2 using MiSeq desktop sequencer (Illumina). The sequenced data were analyzed in the Qiime bioinformatics pipeline.