Plasma extracellular vesicle miRNA sequencing in cervical cancer patients under sevoflurane versus propofol anesthesia
This study investigates whether plasma extracellular vesicles (EVs) collected during sevoflurane versus propofol anesthesia carry distinct microRNA (miRNA) profiles that may influence cervical cancer cell behavior. Paired plasma samples were collected immediately before anesthesia induction and before wound closure from 53 patients with stage II cervical cancer undergoing radical surgery (sevoflurane, n=28; propofol, n=25). EV-enriched preparations were isolated by differential ultracentrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and immunoblotting. For small-RNA sequencing, a representative subset of 12 EV samples was selected: three randomly chosen patients from the sevoflurane cohort and three from the propofol cohort, each providing paired pre- and post-anesthesia EV samples. Libraries were prepared and sequenced on an Illumina platform. The raw sequencing data (FASTQ files) are deposited in the SRA under this BioProject.