Expression data from cultured mouse spermatogonia cryopreserved on the International Space Station (ISS) or on Earth
Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes by supplementing GDNF and FGF2 (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and their gene expression profiles were analysed. Overall design: GS cells were established from testes of 2-3 day old mice of the DBA/2 strain as previously described (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). Cells were cryopreserved for 6 months in ISS or on Earch for 6 months. Total RNA samples were extracted with TRIzol reagent (Invitrogen) and purified using the RNeasy cleanup system (QIAGEN, Valencia, CA). The RNAseq libraries were generated using the  Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA). Sequencing was performed on an Illumina NextSeq 2000 platform with the paired-end mode. In this dataset, we include the expression data obtained from cultured spermatogonia (GS cells) derived from transgenic mouse line C57BL6/Tg14(act-EGFP-OsbY01) that was bred into DBA/2 background. Each group contains 3 biological replicates.