Low_DEN_treatment
This work complements our whole genome crispr screen which has identified several genes that seem to have a positive effect on cellular fitness in vitro.  We wish to investigate if these genes are still under positive selection in vivo.   We have exposed bl6 mice to a low dose of diethyl-nitrosamine and then aged them.  2mm2 pieces of oesophageal epithelium will be sequenced at high depth using a targeted bait panel of predicted driver genes.