Rhopalosiphum padi Whole Body Diurnal and Circadian Transcriptome on Wheat and Artificial Diet
Two separate experiments were conducted on wheat and artificial diet. In each experiment, aphids were exposed to a 48-h pre-entrainment period of either light:dark (LD) or continuous darkness conditions (DD). In both experiments aphids were collected at 4-h intervals over a 48-h period. In total, 13 timepoints were sampled. At each timepoint, 4 biological replicates containing 5 apterous whole body adults were collected. Sampling began at the onset of the first dark period following light treatment entrainment, termed -8 zeitgeber time (ZT) for the LD treatment or circadian time (CT) for the DD treatment. RNA was extracted using the Quick-RNA Miniprep Kit (Zymo Research, Irvine, CA) and sent to Novogene Corporation Inc. (Sacramento, CA) for mRNA enriched library preparation and paired-end RNA sequencing (2x150 bp) on the NovaSeq X Plus platform (Illumina Inc., CA, USA). Please see associated manuscript for more detailed information on methodologies used to generate this dataset.