Loss of function of chromatin remodeler OsCLSY4 led to RdDM-mediated mis-expression of endosperm-specific genes effecting grain qualities [CLSY4_bisulfite_PCR]
Knock down of CLSY4 in rice caused endosperm development defects. We investigated the molecular effects of loss of CLSY4 in rice  endosperm by profiling sRNA, targeted bisulfite PCR,whole genome bisulfite sequencing and mRNA transcriptomes .These analyses revealed the roles of CLSY4 in Endosperm Overall design: We have performed targeted bisulfite sequencing to understand the methylation status of leaf upon CLSY4 and CLSY3 knockdown