Circular RNA expression profiling in UVA-treated HaCaT cells
This RNA sequencing dataset analyzes circRNA expression changes in HaCaT keratinocytes after UVA irradiation. Cells were exposed to 5 J/cm2 UVA and profiled using ribosomal RNA-depleted RNA sequencing on an Illumina platform. Differentially expressed circRNAs were identified by computational pipelines. The data provide insights into circRNA regulation during photo-oxidative stress. Overall design: HaCaT cells were cultured in DMEM with 10% FBS and antibiotics. For UVA treatment, cells at 70% confluence were exposed to 5 J/cm2 UVA irradiation using a UV lamp. Control cells were sham irradiated. After 6 hours, total RNA was isolated using TRIzol reagent. Ribosomal RNA was removed and sequencing libraries constructed for Illumina sequencing. Raw reads were aligned to the hg38 human reference genome. Back-spliced junction reads were used to identify and quantify circRNA expression.