Transcription factor induction of vascular blood stem cell niches in vivo [scRNA-Seq.Adult-Kidney]
We report single cell gene expression data for FACS-isolated zebrafish kdrl(flk1):mCherry or kdrl(flk1):GFP positive cells collected from adult kidneys. Overall design: kdrl:mCherry or kdrl:GFP positive transgenic adult kidneys were dissected, homogenized, filtered, and then 25,000 mCherry+ or GFP+ cells were FACS sorted into PBS. 2,000-3,000 cells were then encapsulated using the inDrops method and libraries were prepared for sequencing.