Plasmodium falciparum Raw sequence reads
This study aimed to investigate the genetic landscape of antimalarial drug resistance in Plasmodium falciparum across two ecologically distinct districts in northwest Ethiopia, where concerning resistance patterns have recently emerged. By applying molecular inversion probe (MIP) targeted sequencing to 903 infections collected over one year, the research focused on tracking the prevalence and spread of key K13 mutations associated with artemisinin partial resistance (ART-R), including the rapidly emerging R622I and the globally significant C580Y mutation. In addition to K13 markers, the study examined partner drug resistance mutations and diagnostic escape phenotypes, such as HRP2-based rapid diagnostic test (RDT) negativity. These findings provide critical insight into the evolving drug resistance landscape in the Horn of Africa and underscore the urgent need for enhanced genomic surveillance to inform malaria treatment, control, and elimination strategies.