Microbial diversity in a biotrickling filter performing the anoxic biodesulfurization.
The objective is to assess the effects of high inlet hydrogen sulfide loads followed by periods of hydrogen sulfide starvation on the biotrickling filter bacterial consortium.-NGS sequencing project started with quality control of the PCR products, where the DNA was analysed to ensure samples had sufficient integrity and quantity for optimal amplification.-Library construction was performed using the KAPA HyperPlus Library preparation protocol.-The generated DNA fragments (DNA libraries) were sequenced with MiSeq Reagent Kit v3 in the lllumina MiSeq platform, using 300bp paired-end sequencing reads.The analysis of the generated raw sequence data was carried out using QIIME2 v2019.7.0The reads were denoised using the DADA2 plugin [2]. The following processes were applied:-Trimming and truncating low quality regions-Dereplicating the reads-Filtering chimeras.