DHFR deep mutant library
This project contains deep mutant libraries of the E. coli folA gene (encoding dihydrofolate reductase). These libraries were created to study the adaptive fitness landscape using mass selection experiment. The experiment contained 6 replicated samples which were sequenced in pools: Sample_F01, Sample_F02, Sample_F03, Sample_F04, Sample_F05, Sample_F06. The reads from replicate samples can be de-multiplexed using the following barcodes >Sample_F01 XAGTCATTGAG >Sample_F02 XGATCTCATTC >Sample_F03 XCGCTTATCCT >Sample_F04 XTATCATGCAG >Sample_F05 XATCTGCGTAC >Sample_F06 XGATTGCACGC