SMARTA CD4 T cells from spleen and lamina propria 8 days after infection with LCMV clone-13 on SPF, OMM12 and GF mice [scRNA-Seq 10x]
Comparison of the impact of specific microbiota on mouse CD4+ T cell response during chronic infection using scRNA-Seq. Overall design: Specific pathogen free (SPF), oligo mouse microbiota12-colonized (OMM12), and germfree (GF) mice received 5000 adoptively transferred SMARTA T cells 5 days prior infection with LCMV Cl-13. SMARTA T cells (live/dead? CD4+CD45.1+) cells from small intestine lamina propria and spleen were re-isolated at 8 dpi and subjected to single-cell RNAseq analysis.