Cloning of two NLR genes at YRTD121 locus from wild emmer wheat Raw sequence reads
we use map-based cloning and long-read sequencing to isolate two nucleotide-binding and leucine-rich repeat (NLR) receptors from wild emmer wheat. By mutagenesis, gene silencing, and genetic transformation, we show that genes TdNLR1 and TdNLR2 oriented head-to-head are both required for YrTD121-mediated stripe rust resistance.