N2420119_80-1608405256_Delivery_20241031092910
First, the raw data is processed by removing adapters, filtering low-quality reads, and then assembling. Subsequently, chimeric sequences are removed to obtain valid sequences. Clustering analysis or denoising analysis is then performed on these valid sequences to obtain OTUs (Operational Taxonomic Units) / ASVs (Amplicon Sequence Variants). In fact, both are artificially defined operational taxonomic units. Taxonomic analysis is conducted on the representative sequences of OTUs/ASVs to obtain information on the species distribution of each sample.