MiRNA expression in a patient-derived CRC model after treatment with TGFB1
We determined the miRNA expression changes upon activation and inactivation of the TGFB signaling cascade Overall design: We treated a patient-derived CRC spheroid model with recombinant TGFB1 or a vehicle control for 7 days, and let them recover for additional 7 days in the absence of TGFB1.  Here, we analyzed the miRNA expression pattern by small RNA sequencing and determined miRNAs expression in all conditions.