Absolute Photo-Formatotrophy in Purple Non-Sulfur Bacteria Enables High-Efficiency One-Carbon Bioconversion
Genome-wide transposon mutagenesis coupled with Tn-seq was performed to identify genes essential for photoformatotrophic growth in Rhodopseudomonas pseudopalustris DSM 123. A pooled library of barcoded transposon insertion mutants was constructed and cultivated under photoformatotrophic conditions using formate as the sole carbon source.