A comprehensive analysis of microRNA alteration in ApoE(-/-) mice model of white adipose tissue injury induced by chronic intermittent hypoxia
In the current study, we first used miRNA-seq technology to identify the miRNA expression profile of CIH-induced WAT dysfunction in a mouse model. Then, Gene Ontology (GO) and KEGG analyses were utilized to analyze the differential expression of mRNA targeting function, followed by RT-qPCR validation. Finally, we constructed a regulatory network for miRNA–mRNA based on the above results. This study contributes to a more detailed description of the WAT dysfunction mechanism associated to OSA. Overall design: we established an apolipoprotein-deficient(ApoE-/-) CIH mouse model and identifed  differentially expressed miRNAs (DEmiRs) using miRNA_x0002_seq technology. With the help of Gene Ontology (GO) functional enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, we determined the biological functions of these DEmiRs. In addition, RT-qPCR was performed for further evaluation of the sequencing data. Finally, we constructed a conserved negative correlation (CNC) network to expound the relationship between miRNA and target genes.