Chromatin Immunoprecipitation Sequencing of histone modifications (H3K4me3 and H3K27ac) in mouse macrophages (RAW264.7) infected with Mycobacterium tuberculosis
Histone modifications plays vital role in the regulation of immune response following Mycobacterium tuberculosis infection. Here, mouse macrophages (RAW264.7) were infected with M. tuberculosis (H37Rv) or left uninfected, followed by Chromatin immunoprecipitation with anti-H3K4me3 or anti-H3K27ac antibody and high throughput sequencing. Overall design: Chromatin Immunoprecipitation Sequencing of H3K4me3 or H3K27ac modification in M. tuberculosis (H37Rv) infected (6 hrs and 20 hrs) and uninfected mouse macrophages (RAW264.7).