In vivo CRISPR screens for Toxoplasma using endomembrane-nucleus sublibrary in WT and Ifngr1-deficient  mice
We performed in vivo CRISPR screen of Toxoplasma targeting endomembrane and nucleus-related genes to identify in vivo fitness genes in WT and Ifngr1-deficient mice. Overall design: This sublibrary targeting 395 genes which consisted 3945 gRNAs targeting endomembrane and nucleus-related genes together with dispensable and essential control genes. We performed in vivo CRISPR screens to identify novel virulence factors of Toxoplasma.