Distinctive Biological Properties Between Mesenchymal Stem Cells Spheroids and Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes in 3D Culture Systems.
mRNA sequencing of mesenchymal stem cells in 2D culture systems, mesenchymal stem cells spheroids and mesenchymal stem cells/extracellular matrix in 3D culture systems to profile gene expressions Overall design: Total RNA was extracted from three distinct cell culture groups: (1) 2D cultures in 24-well plates seeded at 2.0×105 cells/well (n=3), (2) a set of eight MSC spheroids, each consisting of 2.5×104 cells (n=3), and (3) a single C-MSCs formed from 2.0×105 cells (n=3). RNA extraction was carried out using RNA-iso (Takara, Otsu, Japan) and the concentration of the extracted RNA was quantified spectrophotometrically at wavelengths of 260 and 280 nm. The construction and sequencing of the cDNA library were carried out by the Beijing Genomics Institute using the DNBSEQ platform. This project contains 9 samples of (3 from MSCs in 2D culture systems, 3 from MSCs spheroids and 3 from C-MSCs) RNA-seq data.