Determination of transcriptomic changes associated with heat stress in ethanol treated vs non-treated control tomato plants II
We report the gene expression profiles of tomato seedlings and fruits treated with or without ethanol under heat stress conditions. Overall design: Seeds of a tomato (Solanum lycopersicum L.) cultivar, “Micro-Tom” were grown in pots containing horticultural cray granule soil at 22 °C with a 16 h light (110 to 140 µmol m-2 s-1 photon flux density) / 8 h dark cycle. Sixteen-day-old plants grown in pots were used for ethanol pretreatment. The bottoms of the pots were dipped into 20 mM ethanol solution and kept for 3 days. After ethanol pretreatment, the pots were placed in an air incubator set at 50 °C and maintained for the indicated time periods as heat stress treatment. During the heat stress treatment, the pots were electrically rotated in the air incubator to avoid the local effect. After the heat stress treatment, the pots were returned to the growth room and maintained for the indicated time periods.