single-cell RNA sequencing dataset of intestine tissue from weaning and suckling piglet samples
We selected three litters with 6 piglets in each litter. After 21 days of lactation, the piglets from same litter were divided into two groups , the PWP was transferred to a nursery house while getting isolated from the mother sow, whereas NSPs were still nursed by mother sow. The PWP group was provided with ad libitum access to solid food pellets. At an age of 26 days, piglets were euthanized with an intravenous injection of sodium pentobarbital solution. Mid segments of the ileum were obtained immediately and processed for single-cell RNA sequencing. Libraries were subjected to the Illumina NovaSeq 6000 sequencing platform to achieve 52311 reads per cell.