Single-cell RNAseq of control and COMT-KO glioma tumors in mice.
Next-generation sequencing (NGS)-derived transcriptomic profiling was carried out to compare the transcriptomic changes mediated by COMT knockout CT2A glioblastoma tumors using an orthotopic mouse model. Overall design: Here, we collected 7 control glioma tumor samples on Day 12 and 3 COMT-KO samples on Day 12 and 3 samples on Day 25 from mouse brains. CD45 positive immune cells were sorted and subjected for single-cell RNAseq. Individual samples were labeled with single-cell mutiplexing anitbodies and pooled for sequencing.