Map-based cloning and characterization of clubroot resistance genes in Brassica nigra plants
To characterize the clubroot resistant (CR) genes identified in Brassica nigra materials and utilize for CR breeding in canola (B. napus), this project has three main steps. First, isolate candidates of CR genes through map-based cloning; second, transfer the CR genes into canola through Agrobacterium-mediated transformation; lastly, identify the protein repertoire of clubroot pathogen interacting with CR genes.