Gene expression of normal airway epithelium and squamous in homeostasis.
Bulk RNA sequencing analysis was performed to investigate transcriptional profiles in normal airway epithelium and squamous. Overall design: For squamous cells differentiation, we treat the basal cells with 2 uM CHIR99021  during ALI differentiation and valiated with KRT13 staining. Then we collect the RNA to perform bulk-RNA sequencing.