Effect of obesity and metformin on cancer cells of subcutaneous Lewis lung carcinoma cell-line tumors in mice
Male, 2-3-month old, C57BL/6J mice were treated with high-fat diet for about 3 months to induce obesity. Age/sex-matched control mice were fed normal diet. Cohorts from obese and non-obese groups (continued on same diet) were injected subcutaneously (s.c.) with Lewis lung carcinoma (LLC) cells and subsequently monitored until established tumors were noted (~day 10), when mice were injected intra-peritoneally every two days with metformin (50 mg/kg in phosphate-buffered saline [PBS]) or PBS alone. Tumors of mice (n = 5 for each of 4 groups) were harvested after ~3 weeks of cell injection in mice. CD45- EpCAM+ cells were collected from the tumors after dissociating them in vitro. Total RNA isolated from the cells was subjected to RNA sequencing, with libraries were prepared using KAPA mRNA HyperPrep kit. Demultiplexed fastq data are provided here; the data are adapter-trimmed with bcl2fastq.