AoMedA has complex regulatory relationship with AoBrlA, AoAbaA and AoWetA in conidiation, trap formation, and secondary metabolism in nematode-trapping fungus Arthrobotrys oligospora
Transcriptomic analysis revealed that differentially expressed genes in the absence of AomedA and AoWetA were involved in mycelial growth, development process,conidiation, cell nucleus, trap formation, peroxisome, endocytosis and lipid metabolism. Overall design: WT (represented by AO and AOB) and ?AomedA (represented by MedA) and  ?AowetA (represented by WetA) strains grown on cellophane-overlaid CMY plates at 28°C for 5  days, three replicates were used for each sample. After collecting the samples, RNA was extracted with the AxyPrep Multisource RNA Miniprep Kit (Axygen, Jiangsu, China), and then sent to Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) to construct the transcriptome of WT and ?AomedA and ?AowetA strains. The final cDNA library was sequenced on an Illumina Novaseq 4000 platform.