RNA-Seq of PARPi-resistant ovarian cancer cells
We have previously established an in vitro model of PARPi-resistant ovarian cancer by long-term exposure of UWB1.289 ovarian cancer cells (and their isogenic derivatives UWB1.289+BRCA1) to incrementally ascending olaparib concentrations. After finalizing this model, we performed RNA-seq, in order to identify differentially expressed transcript in the PARPi-resistant cells, with a focus on genes related to DNA-repair, multi-drug resistance and EMT. As a result, we show that the phenotype of PARPi resistance is associated with EMT-like traits and up-regulation of selective multi-drug related transcripts. Overall design: UWB1.289  / UWB.1289+BRCA1 ovarian cancer cells and their isgenic PARPi-resistant derivatives (Olres-UWB1.289  / Olres-UWB.1289+BRCA1) were culturred under drug-free conditions and subjected to RNA-seq