A TLR4/Traf6-dependent signaling pathway mediates NCoR co-activator complex formation for inflammatory gene activation [ChIP-seq]
To investigate the genomic localization of NCoR/HDAC3/PGC1ß complex and the enhancer/promoter activity in inflammatory genes, we used NCoR KO or PGC1ß KO bone marrow-derived macrophages and Traf6 siRNA knockdown bone marrow-derived macrophages. We then performed chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K27ac, NCoR, HDAC3, PGC1ß, ERK1, p65, Fosl2, PU.1 and p300. Overall design: Genomic localization of NCoR/HDAC3/PGC1ß and transcription factors with H3K27 acetylation depending on TLR4 signal