BulkRNA sequence for human breast cancer FFPT tissue; TLS-poor and TLS-rich
The formation of tertiary lymphoid structures (TLSs) in tumors is significantly correlated with prolonged patient survival, better prognosis, and improved response to immunotherapy. To assess the tumor microenvironment associated with TLS formation, we performed bulk RNA sequencing using archived FFPE human breast cancer tissues. Overall design: Patient samples of FFPE tissues were categorized as TLS-poor or TLS-rich based on the histology of H&E staining. RNA was isolated from sections of these tissues and used for RNA sequencing