Impact of USP28 monoallelic deletion in HG3 CLL-derived cells
To investigate the role of USP28 expression in chronic lymphocytic leukemia, we used the CRISPR/Cas9 system to engineer isogenic HG3 cell lines harboring a monoallelic USP28 loss. We then analyzed the transcriptomic profile of these CRISPR/Cas9-generated cell lines by RNA-seq Overall design: sgRNA designed to target USP28 exon 4 was used for CRISPR/Cas9 experiments, and a non-targeting sgRNA was used as a control. In total, 2 HG3 WT/WT and 2 USP28 WT/KO independent clones were subjected to RNA-seq and subsequently analyzed for differences in gene expression profile.