Effect of blocking A2aR in sepsis on the Treg transcriptome
In our study, blocking A2aR with ZM241385 was found to inhibit Foxp3 expression in Treg cells of septic mice. Therefore, we used flow cytometry to sort mouse spleen CD4+CD25+ cells for transcriptome sequencing in an attempt to discover the mechanism by which blocking A2aR affects Foxp3 expression. The specific groupings were as follows: naive groups (N1-N5), CLP groups (C1-C5), CLP+A2aR antagonist groups (Z1-Z6). Overall design: This part of the data consists of the following three sections: splenic Treg cells from normal mice, splenic Treg cells from mice with sepsis CLP model, and splenic Treg cells from mice with sepsis CLP model treated with ZM241385.The Treg cells were labeled with CD4+CD25+ antibody and sorted using flow cytometry.