Genome Wide Impact oh human DBR1 depletion on RNA processing
The RNA lariat debranching enzyme DBR1 is essential for intron turnover and RNA metabolism, yet itsbroader impact on transcriptome regulation remains incompletely defined. To elucidate the consequencesof DBR1 depletion, we performed transcriptome-wide RNA sequencing of DBR1 knockdown and wildtype HEK293 cells. Differential expression analysis revealed widespread perturbations in pathways linkedto RNA splicing, mRNA surveillance, translational control, and stress-granule biology. Many of the mostsignificantly altered transcripts encode splicing factors and RNA quality-control components,underscoring DBR1s influence on post-transcriptional regulation.