Genome-wide Mapping of GATA4 in Breast Cancer MCF-7 Cell Chromatin State.
To investigate the functional role of GATA4 in breast cancer cells, we employed ChIP-seq analysis to identify GATA4's genome-wide transcription targets. The ChIP experiment began with the use of anti-GATA4 antibodies in MCF-7 cells. Subsequently, GATA4-related DNA was unbiasedly amplified, labeled, and sequenced. Using Illumina Novaseq 6000, we identified numerous GATA4-specific binding peaks. We observed a strong enrichment of GATA4 on the promoters of specific genes involved in classical pathways. This study has provided us with new insights into the role of GATA4 in chromatin state and gene transcription in the context of breast cancer. Overall design: Examination of  GATA4 target genes in MCF-7 cell line