Targeted Bisulfite Sequencing of Select Human tRNA genes in pooled DNA Samples From Peripheral Blood
Targeted Bisulfite sequencing of 8 pools of DNA from 4 time points for a total 190 individuals.Samples were from two cohorts, MAVIDOS: http://dx.doi.org/10.1016/S2213-8587(16)00044-9 (~4 and ~28 year timepoints) and Hertfordshire: https://doi.org/10.1093/ije/dyi127 (~63 and ~78 year timepoints)tRNA Loci targeted, primers and individual ages are listed in additional metadata on github: https://github.com/RichardJActon/tRNA_paper_code/tree/master/herts-mavidos_targeted_BiS-seqPCR was carried out using a fluidigm 48.48 array.Two batches of Illumina sequencing were carried out for different tRNA genes with some overlap for sites which did not get good coverage in the first round.Primers designed with MethPrimer: http://www.ncbi.nlm.nih.gov/pubmed/12424112