RNA-seq data for studying the influence of fallopian tube stromal cells and/or estrogen treatment on fallopian tube epithilial cells
This study aims to investigate the role of fallopian tube (FT) stromal cells (SCs) in supporting the growth, proliferation and differentiation of fallopian tube epithelial (FTE) cells in response to estrogen signaling. Estrogen receptor alpha (encoded by Esr1) positive cells are present in both FTE and FT stromal compartments. Thus, estrogen exposure can affect FTE cells either directly or indirectly via Esr1+ SCs. Here we used RNA-seq expression profiling of murine FTE cells under various treatment conditions to demonstrate how estradiol (E2) treatment affected their transcriptomics directly, and indirectly via the co-cultured FT SCs, utilizing an organoid culture/co-culture system. Overall design: Wild-type (WT) and p53/Brca1/Pten-null (PBP-null) FTE organoids were established via serial passages in BET (B27, EGF, A83-01) medium. They were then either cultured alone or co-cultured with MACS-sorted CD24- stromal cells from oviducts of WT donor mice, and treated with or without 1nM of E2. After 7 days, EpCAM+ FTE cells were sorted from each organoid samples before proceeding to bulk RNA-seq.