RNA-seq of primary islets in WT and Brsk2 transgenic mice
We investigated the underlying molecular mechanism of Brsk2's effects by comparing gene expression of whole primary islets isolated from Brsk2 TG mice and their control littermates by RNA sequencing.We chosed a four-day Dox administration and studied gene-expression differences resulting in hyperglycemia. A total of 23318 gene transcripts was captured,and 1020 genes showed significant expression differences (p-value<0.05 and >2-fold change) between the two group.Gene set-enrichment ananlysis was performed using Kyoto Encyclopedia of Genomes algorithms and MSigDB software.The most enriched pathways were linked to pathyways associated with olfactory tranduction, ribosomes, and pancreatic secretion. We focused on olfactory transduction because 1122 dysregulated genes are involved in that process. Overall design: Islets mRNA of WT and Brsk2 over-expression transgenic mice