Soil and plant fungal metagenome of Thinopyrum intermedium and Triticum aestivum Targeted loci environmental
Fungal metagenome of Thinopyrum intermedium (intermediate wheatgrass, kernza) and wheat (Triticum aestivum) was determined in plant tissues and rhizosphere soil. Samples were collected at experimental fields in Belgium, France, and Sweden and ITS1 sequence region was amplified on metagenomic DNA using ITS1 and ITS2 primers to asses total fungal metagenome. Arbuscular mycorrhizal fungi (AMF) were studied by sequencing 18S rRNA fragment amplified in two PCR rounds: first with AML2 - NS31 primers and second with AMV4.5NF - AMDGR.