Eukaryotic Initiation factor 4B is a multi-functional RNA binding protein that regulates histone mRNAs
In diffuse large B-cell lymphoma (DLBCL) poor outcome is associated with eIF4B-dependent stimulation of the DEAD box helicase eIF4A that unwinds RNA structures in 5' untranslated regions (UTRs) in mRNAs that encode proteins with a role in cell proliferation. We carried out iCLIP to examine differences in RNAs bound by eIF4B in DLBCL-derived versus -control B-cells. Overall design: iCLIP analysis of eIF4B binding in DLBCL (DoHH2) (n=4) and non-cancerous B-cells (GM01953) (n=2)