Diurnal RNA-Seq for Petunia hybrida
To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, RNA extracted from corolla in the morning (7AM) and evening (7PM) was sequenced to determine genes differentially expressed at the two time points. Overall design: Three replicates of RNA were sequenced and aligned to the Petunia axillaris reference genome to determine transcript levels at 7AM and 7PM.