Genome wide CRISPR screen in AML chemotherapy resistance.
Chemoresistance is the leading cause of acute myeloid leukemia (AML)-related deaths, and elucidation of the mechanisms of AML chemoresistance is necessary to effectively target this process. Here, we performed genome wide CRISPR-Cas9 screening to identify key molecules regulating AML chemoresistance. Overall design: sgRNA counts from THP-1 hCas9 cells infected with two lentiviral gRNA libraries challenged with IDA treatment (1.5 ng/mL) or kept in regular medium.