Methodological test of MIG-seq analysis from noninvasive DNA obtained mammal feces
Multiplexed inter-simple sequence repeat genotyping by sequencing (MIG-seq) is accepted as a powerful tool for clarifying the genetic diversity, and genetic population structure of wildlife because low quality DNA and any species can be used. However, to the best of our knowledge, no study of MIG-seq analysis using fecal samples has been conducted to date.  Thus, we tested a method for MIG-seq analysis using fecal DNA of wild boars Sus scrofa (rectal and trap feces) by obtaining and securing a sufficient amount of DNA for MIG-seq and then extracting only varied SNPs by comparing with the host DNA as a reference.