ChIP-seq identification of HelD (MSMEG_2174) binding sites in Mycobacterium smegmatis
HelD (MSMEG_2174) associates with RNA polymerase in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strain with HelD-FLAG (LK2589), where an additional copy of HelD under anhydrotetracycline inducible promoter was stably inserted into M. smegmatis genome. HelD-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.