Comprehensive Transcriptome Profiling Unveils the Oncolytic Mechanisms of Newcastle Disease Virus on Colonic Carcinoma
Viral oncolysis is supposed to be a promising cancer treatment method because of its good tolerability and durable anti-tumor effects. Compared with other oncolytic viruses, newcastle disease virus (NDV) has some distinct advantages, namely, NDV is a RNA virus which can not recombine with the host genome, making it safer compared to DNA viruses and retroviruses; NDV can induce syncytium formation, allowing the virus to spread between cells without exposure to host neutralizing antibodies; because of hexamer genetic code rule, NDV genome has an lower rate of gene recombination and higher genetic stability. This study systematically investigates the oncolytic mechanism of NDV against colonic carcinoma on transcriptional level and provides theoretical foundations for developing NDV-based therapies targeting colon cancer. Overall design: Human colonic carcinoma cell line HCT116 cells are infected with oncolytic NDV Lasota strain at a MOI of 5. The transcription profils of infected and uninfected cells were generated and compared.