Microalgal-bacterial co-culture transcriptomics
Three axenic host microalgae, Isochrysis galbana, Tetraselmis suecica, and Conticribra weissflogii, were co-cultured with two bacterial isolates, a Yoonia sp. and a Maribacter sp., to study their interactions. After 4 or 6 days, RNA was extracted from cultures using the RNeasy PwerPlant Kit (Qiagen), DNA removed with the Turbo DNA-free Kit (Invitrogen), rRNA depleted, and all mRNA was PE150 sequenced using Illumina Novaseq 6000.