Ostrinia scapulalis 454 transcriptome
The aim of this study was to create transcriptome resources for the lepidopter sibling species Ostrinia scapulalis (adzuki bean borer) and O. nubilalis (European corn borer). This BioProject sample entry holds the raw and assembled data of the O. scapulalis transcriptome. O. scapulalis samples were collected on mugwort in northern France. RNA was extracted from a pool of heads and thorax of four adult moths (two males and two females) and postprocessed. The synthesized cDNA was normalized by one cycle of denaturation and reassociation. After separation of reassociated ds-cDNA the remaining ss-cDNA was amplified with 13 PCR cycles. The normalized cDNA library (range of 500-700bp) was sequenced in one GS FLX Titanium (Roche 454) standard chemistry run (1/4 plate). The raw sequences were cleaned (adapter sequences, species barcodes, ribosomal sequences and too short sequences were removed) and then assembled with Newbler (v. 2.5.3).