Human airway eptithelial cell subsets exhibit distinct transcriptional profiles in response to IFN-gamma derived from influenza specific CD8 T cells.
To evaluate the impact of IFN? secretion by influenza-specific CD8? T cells on human lung epithelial cells, we stimulated total primary human lung cells with influenza peptides in the presence or absence of an IFN?-blocking antibody. Following stimulation, epithelial cells (CD45?, CD31?, CD326?) were isolated by FACS and analyzed by single-cell RNA sequencing. Three stimulation conditions were compared including unstimulated, stimulated with influenza peptides plus isotype control antibody, and stimulated with influenza peptides plus anti-IFNg antibody. Overall design: scRNAseq of airway epithelial cells comparing three stimulation conditions. Total epithelial cells were divided into subsets based on key transcription factors post sequencing.