Autocrine ECM molecules establish MSC quiescence during incisor development by disrupting WNT ligand trafficking process
We used single-cell sequencing to understand the development of incisor tissues from mice. We utilized the single-cell sequencing platform from SeekGene Biotechnology to study the heterogeneity of mesenchyme in incisors at both embryonic and adult stages of mice, and discovered that Smoc2 is a marker gene for mesenchymal stem cells in mouse incisors. We employed the 10X platform to sequence the incisor tissues of smoc1/2 knockout (ko) and control mice during the embryonic stage to investigate the molecular mechanisms underlying the developmental abnormalities in smoc1/2 ko mice.Our study provides a molecular mechanism explaining how stem cells form during development. Overall design: Single-cell transcriptomes were obtained from the digestion of the incisor pulp at different stages (E13.5, E14.5, E15.5, E16.5, E18.5 and P56) and group( control and Smoc1/2 ko).