TRPM1 APA-dependent transcript truncation affects mir211 action
Here we show that U1-dependent premature cleavage and polyadenylation by an RNA surveillance mechanism termed telescripting dysregulates miRNA biogenesis in melanoma. When U1 is reduced, miR-211 levels are decreased as a direct consequence of activation of a newly identified alternative intronic polyadenylation signal located upstream of miR-211 within its host gene, the cation channel TRPM1. Both, miR-211 and TRPM1 levels have previously been correlated with melanoma aggressiveness, but their co-regulation had not been understood.