The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between IAA or dmso treatment in the SMARCE1 - AID system
SMARCE1 binding on mitotic chromatin is required for the timely reinitiation of expression of SMARCE1-bookmarked genes and maintaining identity memory in cell division. Overall design: To examine the effect of mitosis-specific degradation of SMARCE1 on transcription reactivation following exit from mitosis, we used 5- ethynyluridine (EU) to pulse-label nascent transcripts in SMARCE1-AID IAA treated or dmso treated ES cells. Pulse-labeled transcripts were analyzed at 0, 30, 60, 90, 120,180 and 240 min after nocodazole washout as well as in asynchronous cells.