Canine high caloric diet akkermansia intervention
Obesity and excess body weight in companion animals represent significant and growing health concerns, with substantial pathophysiological overlap with human obesity. Growing evidence indicates that the health burden of obesity in companion animals may ultimately surpass that observed in humans, as dogs and cats not only share common obesity-associated disorders but also continue to a widening range of species-specific comorbidities linked to chronic inflammation and metabolic dysfunction. The potential benefits of modulating microbiota and host functions have been identified as an attractive dietary approach to counteract the metabolic and inflammatory sequela of excess adiposity. In this diet-controlled study, we investigated the effects of oral administration of pasteurized Akkermansia muciniphila MucT in dogs subjected to a high-caloric diet. Supplementation in healthy beagle dogs reduced weight gain and suppressed the high-caloric diet-induced outgrowth of Peptacetobacter hiranonis and Collinsella spp. compared to controls. Pasteurized A.muciniphila-treated animals exhibited reduced circulating pro-inflammatory cytokines and a marked decrease in fecal calprotectin levels, indicating resolution of mucosal inflammation. These changes were accompanied by normalization of circulating bile acid profiles, more specifically a reduction in microbiome-derived secondary bile acids and oxo-derivatives, both of which are commonly elevated in metabolic dysregulation. Integrative multi-omics factor analysis identified a coordinated host-response signature encompassing inflammatory markers, bile acid metabolism, and metabolic parameters that was strongly induced by high-caloric feeding and significantly attenuated in the pasteurized A. muciniphila-supplemented group. Together, these findings provide integrative insight into the immune-metabolic effects of  pasteurized A. muciniphila MucT in canines and support its translational potential as a dietary intervention to mitigate obesity-related dysfunction in companion animals.