Transcriptional consequences of lactate transporter inhibition during myofibroblast differentiation
Myofibroblast differentiation by TGFb is associated with increased glycolysis. Myofibroblasts play an important role in tissue fibrosis. In this study, we sought to understand the transcriptional consequences of lactate transport inhibition on myofibroblast differentiation. Commercially available human lung fibroblasts (Lonza) were serum starved and then treated with TGFb (2 ng/mL) in combination with MCT1 inhibitor AZD3965 (100 nM), MCT4 inhibitor VB124 (10 uM), or both inhibitors for 48 h. DMSO (0.1%) served as the vehicle control. RNA was harvested from four biological replicates and submitted for RNA sequencing.