Predicting gene level sensitivity to JAK-STAT signaling perturbation using a mechanistic-to-machine learning framework I
To link upstream IL-6 and IL-10-induced JAK-STAT signaling to downstream gene expression in bone marrow derived macrophages (BMDMs). Bulk RNAseq was done on cytokine-stimulated BMDM in order to profile cytokine-induced response. Overall design: We stimulated BMDM with six stimulation conditions (IL-6, IL-10 and in combination at 1 ng/ml and 10 ng/ml) at 1, 2 and 4 hours. Expression was compared to unstimulated BMDM.