GAP Reference Genomes
29 Australian plant species were selected for genome sequencing and assembly that represented taxonomic clades where few or no representatives of reference genomes were available. Long read sequencing (PacBio-HiFi and Oxford Nanopore PromethION) was carried out and genomes assembled by HiFiasm or Canu. Genomes were annotated without RNA data by GeMoMA using NCBI RefSeq annotations and references (nuclear genomes) and by Chloe (plastids) and MFANNOT (mitochondria).  Acknowledgements We would like to acknowledge the contribution of the Genomics for Australian Plants Framework Initiative consortium  (https://www.genomicsforaustralianplants.com/consortium/) in the generation of data used in this publication. The Initiative is supported by funding from Bioplatforms Australia (enabled by NCRIS), the Ian Potter Foundation, Royal Botanic Gardens Foundation (Victoria), Royal Botanic Gardens Victoria, the Royal Botanic Gardens and Domain Trust, the Council of Heads of Australasian Herbaria, CSIRO, Centre for Australian National Biodiversity Research and the Department of Biodiversity, Conservation and Attractions, Western Australia. The authors acknowledge the provision of computing and data resources provided by the Australian BioCommons Leadership Share (ABLeS) program. This program is co-funded by Bioplatforms Australia (enabled by NCRIS), the National Computational Infrastructure and Pawsey Supercomputing Research Centre  Lan Li for lab work, Nigel England for caring for experimental plants (Leucochrysum).  We would like to thank the ACRF Biomolecular Resource Facility at the John Curtin School of Medical Research, ANU in Canberra, Australia, where PacBio Revio and Oxford Nanopore Technologies PromethION sequencing was conducted. This research acknowledges the support provided by NCRIS-enabled Bioplatforms Australia infrastructure.  We would like to thank Genomics WA in Perth, Australia, where PacBio Sequel II sequencing was conducted. This facility is supported by Bioplatforms Australia, State Government Western Australia, Australian Cancer Research Foundation, Cancer Research Trust, Harry Perkins Institute of Medical Research, Telethon Kids Institute and the University of Western Australia. We gratefully acknowledge the Marshall Centre and the Stan Perron Foundation for the generous donation of PacBio Sequel II Sequencer for the use of Genomics WA.  We would like to thank the Australian Genome Research Facility (AGRF, Melbourne) for completing the Illumina sequencing.  This research was undertaken with the assistance of resources from the National Computational Infrastructure (NCI Australia), an NCRIS enabled capability supported by the Australian Government.  We would like to acknowledge and thank Australia's First Nations Peoples, the Traditional Custodians of the land on which the native flora grows. We acknowledge and thank Elders past, present and emerging.   Acknowledgements We would like to acknowledge the contribution of the Genomics for Australian Plants Framework Initiative consortium  (https://www.genomicsforaustralianplants.com/consortium/) in the generation of data used in this publication. The Initiative is supported by funding from Bioplatforms Australia (enabled by NCRIS), the Ian Potter Foundation, Royal Botanic Gardens Foundation (Victoria), Royal Botanic Gardens Victoria, the Royal Botanic Gardens and Domain Trust, the Council of Heads of Australasian Herbaria, CSIRO, Centre for Australian National Biodiversity Research and the Department of Biodiversity, Conservation and Attractions, Western Australia. The authors acknowledge the provision of computing and data resources provided by the Australian BioCommons Leadership Share (ABLeS) program. This program is co-funded by Bioplatforms Australia (enabled by NCRIS), the National Computational Infrastructure and Pawsey Supercomputing Research Centre  Lan Li for lab work, Nigel England for caring for experimental plants (Leucochrysum).  We would like to thank the ACRF Biomolecular Resource Facility at the John Curtin School of Medical Research, ANU in Canberra, Australia, where PacBio Revio and Oxford Nanopore Technologies PromethION sequencing was conducted. This research acknowledges the support provided by NCRIS-enabled Bioplatforms Australia infrastructure.  We would like to thank Genomics WA in Perth, Australia, where PacBio Sequel II sequencing was conducted. This facility is supported by Bioplatforms Australia, State Government Western Australia, Australian Cancer Research Foundation, Cancer Research Trust, Harry Perkins Institute of Medical Research, Telethon Kids Institute and the University of Western Australia. We gratefully acknowledge the Marshall Centre and the Stan Perron Foundation for the generous donation of PacBio Sequel II Sequencer for the use of Genomics WA.  We would like to thank the Australian Genome Research Facility (AGRF, Melbourne) for completing the Illumina sequencing.  This research was undertaken with the assistance of resources from the National Computational Infrastructure (NCI Australia), an NCRIS enabled capability supported by the Australian Government.  We would like to acknowledge and thank Australia's First Nations Peoples, the Traditional Custodians of the land on which the native flora grows. We acknowledge and thank Elders past, present and emerging.