Sertoli cells of Yak and Cattle-yak Genome sequencing and assembly
Total RNA from sertoli cells for each individual (a total of 5 samples) were extracted with the trizol (Ambion/Invitrogen, USA) following the manufacturer's instructions. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). All libraries were sequenced by the Illumina NovaSeq 6000. The clean reads were obtained after the filtration of low-quality reads using standard quality control by Fast P (version 0.19.7) software.