Metagenomics of Environmental Cyanobacterial Biofilms
Environmental cyanobacteria biofilms were collected across  distinct geographical areas, covering distinct habitats, such as marine, freshwater, soil, hot spring and subaerial. The environmental biofilms were collected into falcon tubes and a DNA/RNA Shield solution was added to help preserve the DNA integrity. Samples were stored at -80 °C and eDNA was extracted using PowerSoil® DNA Isolation Kit protocol. Shotgun metagenomics sequencing was performed using DNBseq sequencing technology (PE 150bp) at BGI genomics. For each sample a final 10 GB clean data file was obtained.