Iso-Seq for the barley pan-transcriptome
This project contains PacBio Iso-Seq data provided by the Internal Barley Pan-Transcriptome Consortium. The 20 accessions sequenced for the first barley pan-genome project (Jayakodi et al., 2020, https://doi.org/10.1038/s41586-020-2947-8) were used for transcriptome sequencing. Five separate tissues were sampled for each genotype, which encompassed embryo (including mesocotyl and seminal roots), seedling shoot, seedling root, inflorescence and caryopsis. Three biological replicates were sampled from each tissue type. For the Iso-Seq sample, a single sample from each genotype was obtained by pooling equal amounts of RNA from a single replicate from all five tissues. Each sample was sequenced on an individual SMRT cell to maximise representation of alternative transcripts. Sequencing was carried out using a Sequel II sequencer at IPK Gatersleben. Corresponding Illumina data from each of the biological replicates has been submitted as a separate bioproject, PRJEB64639.