RNA-seq of Arcobacter butzleri RM4018 wild-type and Abu0127 deletion mutant under microaerophilic and paraquat stress conditions
We performed transcriptome (RNA-seq) analyses for Arcobacter butzleri RM4018 wild-type (WT) and Abu0127 deletion mutant (?Abu0127::aphA-3) under oxidative stress and optimal microaerobic growth conditions.  The expression of 290 genes was affected by oxidative stress in stressed wild-type cells (WTS) compared to non-stressed cells (WT). A comparison of genes expressed in the ?Abu0127 and WT strains under optimal growth conditions revealed 779 differently expressed genes. Moreover, transcriptional changes and overall final protein levels correlated across multiple genes.  The data were validated through RT-qPCR and phenotype experiments for selected processes.