Decoding the protein with CD44 of serum-starved and normal nucleus pulposus cells
In our experiment, we found that there was a nuclear translocation of CD44 in the nucleus pulposus cells after serum starvation. In order to further clarify the role of CD44 molecules after nuclear translocation, we analyzed the CD44 binding regions and related genes between serum starved nucleus pulposus cells and normal nucleus pulposus cells by chromatin immunoprecipitation technique. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for two groups : serum starvation group and control group cultured in a 10% serum medium.