Mechanotransductive Immune Modulation by Remote Ischemic Postconditioning in Spinal Cord Injury
This project investigates the therapeutic mechanism of remote limb ischemic postconditioning (RLPostC) in spinal cord injury (SCI). Using integrated approaches including behavioral assessments, proteomics, transcriptomics, and single-cell RNA sequencing, the study identifies Piezo1, a mechanosensitive ion channel, as a key mediator in RLPostC-induced neuroprotection. RLPostC significantly suppresses Piezo1 expression in pro-inflammatory microglia and macrophages, reshaping the immune landscape and promoting neural repair. The dataset supports mechanistic insights into immune remodeling in SCI and provides a resource for understanding Piezo1-targeted interventions.