16S metagenomics analysis of fecal matter of commercial poultry farms of Faisalabad, Pakistan
Fecal samples from 18 poultry farms (twelve layers and six broilers) in Faisalabad, Pakistan were analyzed using 16S rRNA gene sequencing of the V3-V4 region to evaluate bacterial composition. Fecal samples were collected by sterile forceps from 18 different commercial farms: broilers (n = 6) and layer (n = 12) across the district of Faisalabad, Pakistan. Three fecal samples per farm were collected. DNA was extracted with the QIAamp Fast DNA Stool Mini Kit.