Low-cost and high efficient generation of near-complete bacterial genomes through TELL-Seq
Increasing evidences have shown that de novo genome assembly could bring additional insights into genomic variation landscapes beyond traditional NGS analyses. Albeit advent of long read sequencing technologies, it's still expensive and DNA-quality/quantity demanding to generate high quality bacterial genome assemblies. Although it has been shown that TELL-Seq, as one of the emerging synthetic long reads technologies, could generate near-complete bactieral genome assembly, there's not yet any third-party systematic assessment of it in applicational settings. In this study, we showed that TELL-Seq could low-costly and high effectively generate near-complete bacterial genome de novo assembles across CG-content, genome-size and genome-complexity spectrum, in both standard and clinically-isolated bactieral strains.