New HLA-DRB1*04 allele
UsiHere we describe a novel DRB1*04 allele, identified in a a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following: A*01:01 ; 31:01 B*37:01 ; 51:01 C*06:02 ; 14:02 DRB1*04:NVL ; *13:01 DRB3*01:01 DRB4*01:03/124/134/174 DQA1*01:03 ; *03:03 DQB1*03:01 ; *06:03 DPB1*04:01 DPB1*06:01. HLA-DRB1*04 : New allele differs from DRB1*04 :01 by one nucleotide substitution at position 167 of exon 2, where  base C is replaced by T  without amino acid change. In fact, the  60th codon of HLA-DRB1*04:01 (TAC) becomes TAT in this DRB1*04:New, both of them code for Tyrosin.