Total RNA-seq of Day-21 WT and MTR4-null nucleus and cytoplasm
Mtr4 deletion caused unbalanced mRNA distribution between different subcellular components like nucleus and cytoplasm. In order to obtain a global view of the abnormally distributed transcripts, total RNA-seq of nucleus and cytoplasm seperated from Day-21 WT and MTR4-null oocytes were conducted in this study. Total RNA were extracted by TRIzol. rRNA were removed. 10 nuclei or cytoplasms were used per sample.