Enzymatic DNA Synthesis and Rolling-Circle Amplification for DNA Vaccine Production
This study introduces Enzymatic DNA Synthesis and Rolling-Circle Amplification (EDS-RCA), a cell-free method for producing DNA vaccines from text files. We used PacBio sequencing to assess the fidelity of influenza hemagglutinin (HA) constructs made with EDS-RCA and compared their immunogenicity in mice to standard plasmid DNA vaccines.