Activity of Cas9-sg05 RNPs at human IgG genes
Primary human B cells were electroporated with Cas9-sg05 RNPs. Genomic DNA was extracted 5 days later, and targeted amplicon sequencing of the on-target IGHG1 locus, as well as off targets IGHG2, IGHG3, IGHG4, and IGHGP was performed to assess the outcomes of DNA cleavage.