Reverse Transcription Enabled FLEX (REFLEX) Enables High-Throughput Multiomic scRNAseq with AIRR Detection
Comprehensive profiling of T cell repertoire at single cell resolution is challenging due to the scale and cost requirements of capturing the vast diversity that exists. Current technologies struggle to combine TCR capture with sufficient scale, or by contrast, achieve scale but not TCR compatibility. Here, we utilize both neat and virally stimulated PBMCs to showcase REFLEX, a technological approach enabling highly scalable, low cost per cell profiling of TCR repertoire in parallel with epitope and gene expression in fixed cells. This combination of capabilities allows for high throughput, multi-omic profiling of rare T cell subsets and longitudinal study formats at scales that were previously unattainable.