Transcriptome data from U2OS cells and hTERT CRISPR clones
To assess the biological significances of p-hTERT in ALT cells, we used the CRISPR-Cas9 system targeting the hTERT gene and performed RNA-seq to analyze the profile of gene expression in hTERT-CRISPR clones (clone#1 and #2). The subsequent gene ontology (GO) analysis showed enrichment of genes involved in cell cycle process and DNA replication within genes down-regulated in hTERT-CRISPR clones. Overall design: We established two U2OS cell clones with heterozygous mutations in the hTERT gene locus by CRISPR-Cas9 system. Transcriptome data of wild-type U2OS cells and the hTERT CRISPR clones (clone#1 and #2) were obtained by the RNA-seq analysis. Two replicates for each cell.