Single cell RNA-Seq result of  spinal cord of AR-97Q mice and wild-type mice at four disease stages
To understand the early pathogenesis of SBMA and to systematically assess the role of different cells in the spinal cord of SBMA, we conducted snRNA-seq on the spinal cord of AR-97Q mice at different stages of the disease. The genes related to ion channel and synapse function were up-regulated in oligodendrocytes in early stages of SBMA although they were down-regulated at the advanced stage. Overall design: Single-nucleus RNA sequencing (snRNA-seq) was conducted on spinal cord samples from AR-97Q mice and wild-type mice at four stages: pre-pubertal (3 weeks of age), pre-onset (6 weeks of age), early symptomatic (9 weeks of age) and advanced (13 weeks of age). Spinal cords from 4 mice per a sample were pooled for homogenization and nuclear isolation.