RNAseq of malignant peripheral nerve sheath tumors from Nf1 flox/flox Arf flox/flox PostnCre+ mice
Malignant peripheral nerve sheath tumors (MPNSTs) are the leading cause of premature death for patients with Neurofibromatosis type 1 and no approved targeted therapies are available. Transformation from Nf1-null benign plexiform neurofibromas is driven by the loss of the Cdkn2a (Arf) locus. Here, genetically engineered mouse models with combined Nf1 flox/flox and Arf flox/flox alleles were used (crossed with Postn-Cre+ mice). Tissue from MPNSTs that form in the Nf1-/-;Arf-/- setting were used for mRNA sequencing and compared to benign plexiform neurofibroma tissue (Nf1-/- from Nf1 flox/flox; Postn-Cre+ mice, GSE213789) to identify transcriptome signatures from MPNST and compare them to benign plexiform neurofibroma. Overall design: 6 experimental samples (malignant peripheral nerve sheath tumors from 6 Nf1 flox/flox Arf flox/flox, Postn-Cre+ mice, used to compare to plexiform neurofibromas from GSE213789)