ChIP-seq identification of RbpA (MSMEG_3858) binding sites in Mycobacterium smegmatis
RbpA is an essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with RbpA-FLAG (LK2541), where an additional copy of RbpA under anhydrotetracycline inducible promoter was stably inserted into the genome. RbpA-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.