RNA-seq analysis in human REH pre-B lymphoblastic cells in response to XIST-KD
We performed RNA-seq experiments to modulate levels of XIST RNA.  We inhibited XIST activity in REH lymphocytic cells via knockdown of XIST expression by short hairpin (sh) RNA. We confirmed that the RNA level of XIST was greatly reduced in shRNA-XIST cells by both RNA-seq and qPCR analyses, relative to cells transfected with a scrambled shRNA. Overall design: RNA-seq of control and shXIST REH cells