nirS gene-based profiling of denitrifying microbial communities on different MBBR carriers in biological ponds
This project investigated the functional composition of denitrifying microbial communities colonizing three different MBBR carriers, with each carrier type installed in an indentical biological pond. The carriers were a conventional MBBR, an MBBR carrier amended with an iron-based substrate (MBBR+VIA), and an MBBR carrier amended with a sulfur-iron-based substrate (MBBR+SIA). Three replicates were collected from each type of carrier, designated as B1-B3, C1-C3, and D1-D3, respectively. The nirS gene was amplified using primers Cd3aF/R3cd, followed by high-throughput sequencing of the amplicons.