Enrichment of mixed anaerobic cultures to improve beneficial interactions with Methanosarcina.
Mixed cultures already enriched for DIET (Giangeri et al., 2025, Chemical Engineering Journal) were used to enrich beneficial interactions with the main methanogen, Methanosarcina. Based on the auxotrophies, vitamins, amino acids and carbon sources were supplemented to enrich 5 MAGs of interest. Their abundance was followed by qPCR and 16S rRNA gene sequencing every 15 days for three months. Shotgun metagenomics was also done on the initial inoculum and on the main samples of interest at the end of the three months.