16S absolute quantification project of microbial diversity
The Accu16STM bacterial absolute quantification method involves adding a known amount of spike-in standards with synthetic sequences to the sample DNA. This is followed by 16S amplicon library construction and sequencing. The absolute copy number of the 16S rRNA gene for species within the standard curve range is calculated based on the number of 16S amplicon reads corresponding to the spike-in standards and the known copy number. The Accu16STM bacterial absolute quantification high-throughput sequencing method effectively addresses the issue of absolute quantification of microorganisms in environmental samples, while also providing information on the species composition within those samples.