Metagenomic sequencing of microbial diversity
Under greenhouse conditions, resistant clubroot variety HYZ5R (Huayouza 5R) and susceptible variety HYZ62 (Huayouza 62) were subjected to inoculated and uninoculated treatments with Plasmodiophora brassicae. Rhizosphere soils were collected 4 weeks post-inoculation for high-throughput sequencing of bacteria (using primers 338F/806R) and fungi (using primers ITS1F/ITS2R). The experiment comprised four treatments: uninoculated HYZ62, inoculated HYZ62, uninoculated HYZ5R, and inoculated HYZ5R, with six replicates per treatment.