Integrated ATAC-seq and RNA-seq analyses of human HaCaT keratinocytes during chronic UVB-induced malignant transformation
This project reports genome-wide chromatin accessibility profiling by ATAC-seq and transcriptome profiling by RNA-seq in human immortalized keratinocytes (HaCaT) subjected to long-term, chronic UVB irradiation versus non-irradiated control cells. The study aims to identify dynamic changes in chromatin accessibility and gene expression during UVB-induced malignant transformation toward keratinocyte carcinoma. Key transcription factors and downstream target genes involved in this process were characterized. These data provide a comprehensive epigenetic and transcriptomic resource for understanding the molecular mechanisms underlying chronic UVB-driven skin carcinogenesis.