Transcriptomic profiling and Micro-C mapping of fine-scale chromatin interactions in 2La or 2L+ fixed Anopheles coluzzii hemocyte-like cell lines
Bulk RNA-seq and Micro-C were performed on basally growing SUA4.0 (2La) and Ag55 (2L+) Anopheles coluzzii hemocyte-like cell lines to identify differentially expressed genes and to characterize differential genome-wide chromatin interactions between the cell lines of alternate 2La inversion karyotype. RNA-seq libraries were generated for 4 biological replicates and sequenced on an Illumina NovaSeq platform to generate 2x150bp paired end reads. Micro-C libraries were generated for 3 biological replicates and sequenced on an Illumina NovaSeq platform to generate 2x150bp paired end reads