Identification of a new HLA-B*51 allele in a french  volunteer bone marrow donor
Here we describe a novel HLA-B*51 allele, identified in a a french  volunteer bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following:  A*02:01 A*29:02 B*51:New ,*55:01 C*03:04 ,*14:02 DRB1*11:01 ,*14:54 DRB3*02:02 DRB3*02:02 DQA1*01:04 ,*05:05 DQB1*03:01,*05:03 DPA1*01:03 ,*01:03 DPB1*04:01 ,*105:01 HLA-B*51 New allele differs from B*51 :285  by one nucleotide substitution at position 113 of Exon5 (position 1008 of cDNA) without aminoacide change (312th AGC>AGT both of them code for Ser).