Analysis of Gene Expression in Human Colorectal Cancer HCT116 Cells Treated with Nitidine Chloride
This study aims to analyze the gene expression changes in HCT116 human colorectal cancer cells treated with Nitidine chloride (NC), a compound with potential anticancer effects. The experiment is divided into a control group (0.1% DMSO) and an NC treatment group (6 umol/L), with three replicates per group. Cells in the logarithmic growth phase are collected and seeded in 6-well plates. When the cell confluence reaches around 60%, the medium is replaced with drug-containing medium, and the cells are incubated at 37C with 5% CO2 for 48 hours. Total RNA is extracted using the Trizol method, and samples are sent to Haiyi Times Genetics for whole-genome sequencing. The study aims to investigate the molecular mechanisms underlying the effects of Nitidine chloride on cancer cells, offering insights into its potential as a therapeutic agent for colorectal cancer.