16S rRNA gene sequencing of gut microbiota in cisplatin-induced acute kidney injury mice treated with combined rhubarb and danshen
This study characterizes the gut microbiota composition in a mouse model of cisplatin-induced acute kidney injury (AKI) and evaluates the impact of a combined treatment with two traditional Chinese medicines, rhubarb (Rhei Radix et Rhizoma) and danshen (Salviae Miltiorrhizae Radix et Rhizoma).Male mice were divided into three groups:Normal control (vehicle-treated)Cisplatin-induced AKI model (cisplatin alone)Cisplatin + combined rhubarb and danshen treatmentFecal samples were collected, and the V3-V4 hypervariable region of the 16S rRNA gene was amplified and sequenced using an Illumina platform. Raw sequencing data (FASTQ files) are provided here.These data will be used to assess changes in microbial diversity, community structure, and differential abundance of taxa among groups. The results may reveal potential links between gut dysbiosis and cisplatin nephrotoxicity, as well as the modulatory effects of rhubarb and danshen combination on the gut-kidney axis.