Effect of treatment by BAY1217389 for 48 hr on H1944
We reported that MPS-1 inhibitors activate cGAS-STING pathway in tumor cells by forming micronuclei and inducing cytokine secretion such as type I interferon, CCL5, and CXCL10, thereby promoting immune cell migration and inducing tumor cell shrinkage. In the present study, we performed RNA -seq to search for negative regulators of STING pathway by forcibly activating it in H1944 cells with MPS-1 inhibitors, BAY1217389 and found that the administration of BAY1217389 for 48-hour treatment increased downstream factors of STING pathway and genes involved in antigen presentation, while we found elevation of negative regulators such as TREX1. Overall design: To investigate negative regulators of STING pathway, BAY1217389 or DMSO was administered to the lung cancer cell line H1944 for 48 hours, followed by medium exchange and RNA collection 24 hours later.