WGBS data from a DNA dilution series (K562 in NA12878 background) with pUC19 and Lambda spike-in controls.
The primary objective of this study is to evaluate the performance of whole-genome bisulfite sequencing (WGBS) data, particularly for fusion detection, using a controlled mixture of cell line genomic DNA. A dilution series was prepared by spiking K562 cell line DNA into a background of NA12878 reference DNA at varying known concentrations (2% to 100%) to create a well-defined standard for benchmarking. Additionally, pUC19 (0.1%) and Lambda DNA (0.1%) were included as spike-in controls to monitor bisulfite conversion efficiency and sequencing performance.