Quantitative mapping of pseudouridines in bacterial RNA (Raw sequence reads)
RNA pseudouridylation is one of the most prevalent post-transcriptional modifications, occurring universally across all organisms. Here, we used a bisulfite-based deep sequencing approach to provide a comprehensive and quantitative measurement of bacterial pseudouridines using E. coli, to provide proof of concept. Furthermore, we mapped pseudouridines in oral microbiome samples of human subjects, demonstrating the broad applicability of this approach in complex microbiomes.