Transcriptomic profile of blood cells exposed to particulate matter
Here we used next generation sequencing (NGS), to determine the transcriptional profile of blood cells exposed to particulate matter to contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the inflammation. For this, blood cells from six adult healthy donors were treated with particulate matter. Overall design: Samples we in vitro exposed to particulate matter for 48 hours. RNA was extracted from each sample and forming two groups (treated vs untreated control). Each sample was used to build the sequencing libraries. The cDNA libraries were built with the Kapa Stranded mRNA Sample Preparation Kit and sequencing took place on the NextSeq 500 System platform (Illumina, USA)