Whole body Knockout of PC-TP (Pctp-/-), Hepatic whole RNAseq comparing Wild Type (WT) and knock out mic (KO) as well as Chow Fed and Methionine and choline depleted (MCD) fed mice.
Previous studies have used this model to show that PC-TP has a protective effect when challenged with a methionine and choline depleted diet, known to induce NAFLD. We performed transcription factor binding experiments and show that PPARs are dysregulated in the contect of PC-TP depletion We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 Mice for WT and KO fed either normal chow or methionine and choline depleted chow. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT and KO Livers fed MCD or Chow diet