Whole Transcriptome Sequencing in the blood from Indian Women with and without MDD
The study included six female patients diagnosed with Major Depressive Disorder (MDD) and four healthy female controls. Peripheral whole blood (2.5 ml) was collected in PAXgene Blood RNA Tubes containing RNA stabilization reagent and stored at -20 C after incubation at room temperature for a minimum of 2 hours. High quality total RNA (A260/A280 ratio: 2.10-2.19) with high integrity (RIN > 7.0), as assessed using an Agilent 2200 TapeStation system, was isolated from whole blood samples. cDNA libraries were prepared from total RNA using a modified NEBNext RNA Ultra protocol. The prepared libraries were sequenced on an Illumina HiSeq 2500 platform to generate 2x100 bp paired end reads, producing approximately 50-100 million reads per sample for downstream transcriptomic analysis.