Effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression in HT29 cells
To investigate effect of  WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression and probe possible mechanism of cell viability effect, we overexpressed GFP/CTNNB1, or induce knockdown of APC by shNT1 (non-targeting) and shAPC Overall design: 4 experimental conditions (GFP overexpression, CTNNB1 overexpression, shNT1 ctr, shRNA-APC), in triplicate, of human HT29 tumor cells.