Gene expression of sphere-derived prostate cancer stem cells
We performed RNA-seq analysis of human prostate cancer cells and sphere-derived cancer stem cells (PCSCs) to analyze the differentially expressed genes between PCSCs and differentiated cells, and find the potential key factors in PCSCs Overall design: sphere-derived PCSC cells were cultured with or without serum for 7 days, and then were collected for TRIzol lysed for RNA extraction. 6 RNAseq samples across 2 conditions: sphere, without serum (n=3) and Adh (adherent, with serum) (n=3)