RNA-seq analysis of HEK293 and synphilin-1 overexpressing HEK293 cell line
We report the gene expression profile of HEK293 cells and FLAG-tagged synphilin-1 overexpressing HEK293 cells (Synph-293 cells). Overall design: RNA-sequencing was conducted to compare the gene expression in HEK293 and synphilin-1 overexpressing HEK293 cells (Synph-293 cell). 1 µg of total RNA of each sample was used for preparing the libraries using TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Then, RNAs were fragmented and synthesized as double-stranded cDNA through random hexamer priming. Following the end repair, A-tailing, and adapter ligation, cDNA libraries were amplified with PCR. The quality of cDNA libraries was evaluated with Agilent 2100 BioAnalyzer (Agilent Technologies, CA, USA). The quantification of cDNA libraries was conducted using KAPA library quantification kit (Kapa Biosystems, MA, USA). Following cluster amplification, sequencing progressed as paired-end (2×150bp) using Illumina Novaseq6000 (Illumina).