Single-cell RNA sequencing of Schizosaccharomyces pombe during nitrogen starvation
Nitrogen starvation triggers sexual differentiation and cell fusion in the fission yeast Schizosaccharomyces pombe. To characterize cell-type composition and transcriptional dynamics during this process, we performed single-cell RNA sequencing on a time course of nitrogen starvation (0 h, 2 h, 6 h, 8 h, and 10 h).In addition to the wild-type strain Y450, we analyzed the fusion-defective mutant prm1 delta (Y447) and an evolved strain (Y636) that restores fusion efficiency at 0 h, 2 h, 6 h and 10 h during nitrogen starvation. The dataset provides single-cell transcriptomic profiles for investigating gene expression dynamics, cell-type heterogeneity, and regulatory programs associated with mating and cell fusion in fission yeast.