FOXQ1 Regulates Mitochondrial Cristae Formation and Energy Metabolism in Mouse Brain Endothelial Cells [RNA-seq]
Purpose: To gain  futher insight into how FOXQ1 regulates the brain endothelial cells ,RNA-seq was used to analyze the genome-wide changes resulting from the cerebral cortical endothelial cells  of E18 FOXQ1 conditional knock out mice and littermate wild-type. Methods: Total RNA from E18 Flow sorting of endothelial cells of wild-type(WT) and FOXQ1fl/fl;Tie2-Cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, total RNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 6000 platform in Annoroad Genomics Approximately  one thousand transcripts showed differential expression between the wild-type(WT) and FOXQ1fl/fl;Tie2-Cre mice cortical endothelial cells, with a fold change =1.5 and p value <0.05. These results reflected FOXQ1 plays a role in brain endothelial cells. Conclusions:  RNA-seq based transcriptome characterization would provide a overall understanding how FOXQ1 works in endothelial cells. Overall design: Total mRNA profiles of E18 wild-type(WT) and FOXQ1fl/fl;Tie2-Cre mice were generated by deep sequencing, using Illumina HiSeq 6000.