Spatially-restricted and ontogenically-distinct hepatic macrophages are required for tissue repair.
Analysis of single cell RNA-seq, CITE-seq data and bulk RNA-seq data on cells isolated from the livers of mice following PBS and APAP (paracetamol/acetaminophen) administration. Spatial Transcriptomics analysis was performed using 10x Visium platform on mouse livers 48hrs post PBS or APAP administration. We also analysed single cell RNA-seq data on CD64+F4/80+ macrophages isolated from the livers of Trem2 fl/fl mice and CD64CRE Trem2 fl/fl mice. Overall design: Single cell RNA Seq = Total liver cells derived from mice following PBS and APAP administration and CD64+F4/80+ macrophages from Trem2 fl/fl mice and CD64CRE Trem2 fl/fl mice. Bulk RNA seq on KCs and LAM-like KCs isolated from livers 48hr post PBS or APAP administration 10 Visium Spatial Seq = mouse liver 48h post PBS or APAP administration.