Identification of 5-methylcytosine associated genes in osteoarthritis
Our study aimed to identify common m5C features in OA. We identified we found 35828 m5C peaks and mapped up to 11720 annotated genes in OA samples, and we found 16259 m5C peaks and mapped up to 7504 annotated genes in normal samples, indicating an overall hypermethylated level in the OA cartilage. Overall design: We collected three OA knee cartilage tissues and three normal knee cartilage tissues to obtain the transcriptome-wide m5C modification profile of genes in OA by methylated RNA immunoprecipitation next-generation sequencing (MeRIP-seq).