Spatio-Temporal Study of the Eastern Oyster Microbiome
Seasonality of the Eastern Oyster Microbiome. This study aims to investigate the microbial community structure and functional potential associated with oysters, with a focus on nitrogen cycling and environmental influences, using 16S, ITS, and nosZ gene sequencing. The gut and gill microbial communities of the Eastern Oyster were assessed throughout a one-year growing cycle using the 16S V4-5 region, amplified with NosZ1577F_A(SSCTSAACAAGCTSTCSCA) and NosZ1974R_A(TCSGTSGTCTGCTCGATGT); NosZ1374F_G(CGCTCCACASSCARTTCGA) and NosZ1577R_G(CGGTCCTTSGASAGCTTGT); nosZ1F(WCSYTGTTCMTCGACAGCCAG) and nosZ1R (ATGTCGATCARCTGVKCRTTYTC) primers.