TET2-mediated mRNA in leukemia stemness maintanence and homing [RNA-Seq]
To assess the potential effect of Tet2 on RNA stability, mRNA half-life profiling was performed on samples enriched from AML cells with Tet2+/+ or Tet2-/- genotypes, following treatment with actinomycin D at different time points. Overall design: WT and Tet2-null NRAS/AE9a cells were treated with 5 µg/ml actinomycin D for 0h, 4 h, and 8 h. At each time point, cells were rapidly harvested and total RNA was extracted using TRIzol reagent. The RNA samples were subsequently subjected to sequencing on Illumina NovaSeq 6000 platform with a paired-end 150-base pair (bp) read length.