RNA sequencing of Pseudomonas fluorescens interacting with the roots of Arabidopsis thaliana
To elucidate the global regulatory network governed by RspL, a master regulator of the type III secretion system, in plant growth-promoting rhizobacteria, RNA-seq analysis was performed on Pseudomonas fluorescens 2P24 and its rspL deletion mutant (DrspL). Bacteria solution of strains 2P24 and DrspL were treated with Arabidopsis thaliana roots (R-2P24 and R-DrspL) or not (N-2P24 and N-DrspL) with shaking at 40 rpm at room temperature for 3 h.