Transcriptome analysis during the growth and development of asparagus stems
Asparagus is a nutritionally dense stem vegetable whose growth and development are correlated with its quality and yield. For height of 25 cm asparagus , 17-20 cm is still viable, in which the cells can continue to elongate. When 40 cm, the growth in the 17-20 cm range basically stops. Therefore, we picked periods with significant growth differences for transcriptome comparison to find the dynamic changes and underlying mechanisms during the elongation and growth process of asparagus stems. The results showed that the differentially expressed genes were enriched in many aspects, including the metabolism of plant hormones and carbohydrates. Overall design: To investigate the dynamic changes and underlying mechanisms during the elongation and growth process of asparagus stems, we sampled asparagus stems at different ages. We selected only  asparagus stems of different plant heights with a stem diameter of 13-15 mm, including 10, 25, 40, and 60 cm, with three replicates for each treatment. For different growth and development stages of asparagus, 3-6 cm stem segments from the surface were collected from 10 cm, while 17-20 cm of stem segments from the surface were collected from 25, 40, and 60 cm as the test materials.