Citrus x aurantiifolia cultivar:Colimex Genome sequencing
Mexican lime (Citrus aurantifolia (Christm.) Swingle) is one of the most economically and culturally important citrus crops in Mexico, where it is widely cultivated and highly demanded due to its characteristic acidic flavor and extensive use in traditional cuisine. Here, we present a high-quality genome assembly of C. aurantifolia var. Colimex, a clonal selection widely cultivated in Mexico. The assembly spans 353 Mb distributed across 24 scaffolds and shows an average completeness according to BUSCO of 99.2%. Comparative genomics showed a significant expansion of immune-related genes in Colimex, including 341 NLR. In addition to having a large number of NLR genes, Colimex also has a large number of RLK and RLP receptors. Several biosynthetic gene clusters (BGCs) associated with antimicrobial or specialized metabolites were identified as well. Together, these results provide insights into the genomic basis of defense mechanisms in Mexican lime and establish a foundation for functional genomics studies and breeding strategies aimed at improving disease resistance and productivity in citrus crops.