La Selva Biological Station (Costa Rica) Neotropical lowland rainforest soil DNA metabarcoding of the 18S V4 rRNA
A total of 94 soil samples were collected in the lowland rainforest of the La Selva Biological Station (Costa Rica) in October 2015. All samples were collected and prepared following the protocol of Mahé et al. (2017). General primers (Stoeck et al., 2010) were used to amplify the V4 region of the 18S rRNA. The amplified fragments were sequenced on an Illumina MiSeq v3.Mahé, F., de Vargas, C., Bass, D., Czech, L., Stamatakis, A., Lara, E., … Dunthorn, M. (2017). Parasites dominate hyperdiverse soil protist communities in Neotropical rainforests. Nature Ecology & Evolution, 1, 0091.Stoeck, T., Bass, D., Nebel, M., Christen, R., Jones, M. D. M., Breiner, H.-W., & Richards, T. A. (2010). Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. Molecular Ecology, 19, 21–31.