Single-cell RNA-seq of colonic tumors from epithelial HO-1 knockout and control mice in a colitis-associated cancer model
This dataset contains single-cell RNA sequencing profiles of colonic tumor cells isolated from mice with intestinal epithelial-specific deletion of Hmox1 and littermate controls. Tumors were generated using the azoxymethane-dextran sodium sulfate (AOM-DSS) model of colitis-associated cancer. Following enzymatic dissociation and live cell sorting, single-cell libraries were prepared using the 10x Genomics Chromium platform and sequenced to characterize transcriptional programs within the tumor microenvironment. The dataset was designed to examine genotype-dependent shifts in epithelial transcriptional programs within the tumor microenvironment, with a focus on stress-adaptive responses, iron metabolism, and oxidative stress regulation. Overall design: Colonic tumors were harvested from Hmox1fl/fl (control) and Hmox1?IEC (epithelial HO-1 knockout) mice following AOM-DSS treatment (day 80). Tumors >2 mm were pooled by genotype, dissociated, and sorted for live cells (7AAD). Single-cell libraries were generated using the 10x Genomics Chromium platform and sequenced. Data were processed using Seurat with standard filtering, normalization, and clustering workflows.