RNA-seq analysis reveals host transcriptional responses to WSSV infection under WSV249 knockdown in Penaeus vannamei
This project investigates host transcriptomic responses to White Spot Syndrome Virus (WSSV) infection in Penaeus vannamei under RNA interference (RNAi)-mediated silencing of the viral gene wsv249. Shrimp were injected with dsRNA targeting wsv249 (dsRNA-WSV249), dsRNA-EGFP (control), or PBS, followed by WSSV challenge. Gill tissues were collected at multiple time points post-infection (0, 6, 12, 24, and 48 hours post-infection), and total RNA was extracted for high-throughput sequencing.RNA-seq libraries were prepared using a stranded total RNA protocol with ribosomal RNA depletion and sequenced on an Illumina platform in paired-end mode. The generated data enable characterization of host gene expression dynamics, identification of differentially expressed genes, and functional pathway analysis associated with antiviral responses and viral replication.This dataset supports investigation of the role of the WSSV wsv249 gene, a putative E3 ubiquitin ligase, in modulating host-virus interactions and contributing to viral pathogenicity.