Comparison of miRNA profiles between young (6 months) and old (24 months) male mouse vertebral samples
To understand the effect of age on miRNA profiles in male mouse vertebrae, we isolated L4-5 vertebral samples and digested with endotoxin-free collagenase (Liberase) twice for 30 minutes. The resulting bone was confirmed to be largely osteocyte-enriched and RNA was isolated using QIAzol reagent. Overall design: We then performed miRNA gene profiling between the young (6 month) and old (24 month) vertebral samples.