Transcriptome-wide mapping of RNA:protein interactions of GFP-RS2Z35 and GFP-RS2Z36 in tomato (Solanum lycopersicum) leaves.
We performed iCLIP2 in tomato leaves stably expressing GFP-tagged RS2Z35 or RS2Z36. Overall design: 3 biological replicates were subjected to the iCLIP2 procedure with UV-crosslinking at 254 nm at 750 mJ/cm2. For IPs GFPTrap magnetic agarose used. No UV treatment served as negative control.