Gene expression profile at single cell level of bone marrow derived CD133+ endothelial like cells from young and aged mice bone marrow
bone marrow derived ELCs We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of CD133+ ELCs between young and old. Overall design: Intestinal CD133+ ELCs (approximately 8,000-10,000 cells) of the  young (3-month-old, n=3) and old (18.5-month-old, n=3) mice were isolated by Fluorescence-activated cell sorting (FACS)  and analyzed using scRNAseq.