Changes in gene Expression in different tissues before and after mouse embryonic diapause
To elucidate the characteristics of mural TE and, polar TE, epiblast, and PrE during diapause, we performed laser microdissection followed by RNA-seq analysis. Gene ontology (GO) analysis revealed increased expression of p53- and apoptosis-related genes. p21 was upregulated in diapause embryos, while Txnip was specifically elevated in mural TE cells. Neither gene was expressed in pre-diapause embryos, but both were induced in a diapause-specific manner. Overall design: For tissue-specific RNA-seq analysis, tissues from diapause embryos were separated using the HTlasr system.