Global gene expression (transcriptome) analysis by mRNAseq on iPSC-derived MSCs
Global gene expression (transcriptome) analysis was performed by messenger RNA sequencing (mRNAseq) on iPSC-derived MSCs produced in three separate lots to determine consistency in gene expression between lots. Overall design: Total RNA was extracted from iPSC-derived MSC samples, from which mRNA was then isolated, and analysed using the Illumina HiSeq 2500 platform. Normalised Transcripts Per Kilobase Million (TPM) counts for each sample were then calculated and plotted against one another, and the Pearson correlation was computed to determine the degree of consistency of gene expression and isoforms between samples. contributor: The University of Wisconsin Bioinformatics Resource Center