Transcriptomics of PLXDC2 knockdown BMDM treated with PTEN
To evaluate whether PLXDC2 mediated the role of PTEN on macrophages, PLXDC2 was knocked down in M2-polarized BMDMs, and the resultant PLXDC2-knocked down cells together with control cells were treated with or without PTEN, before subjected to global transcriptome profiling. Overall design: Bone-marrow (BM) cells were harvested from femurs and tibia of C57BL/6J mice. Differentiation was induced by recombinant mouse M-CSF in DMEM medium containing 1% penicillin/streptomycin and 10% FBS for 7 days to obtain the bone marrow-derived macrophages (BMDMs).To induce an M2-like phenotype, we treated BMDMs with IL-4 and IL-13 for 48 h.M2 BMDMs transfected with siNC or siPLXDC2#1,2 were treated with and without 100 ng/mL PTEN for 24 h, followed by RNA-seq analysis.