Transcriptomic analysis of methyl jasmonate-elicited flavonoid biosynthesis in Alpinia purpurata callus cultures: a de novo RNA-Seq investigation
Callus cultures of Alpinia purpurata (Vieill.) K.Schum. (Zingiberaceae) were established from plant material sourced from Rajadhani Agro Farms, Hyderabad, India, and elicited with methyl jasmonate to enhance flavonoid biosynthesis. De novo RNA-Seq transcriptomic profiling was performed on control and methyl jasmonate-treated callus samples to identify differentially expressed genes, flavonoid biosynthetic pathway enzymes, and regulatory transcription factors governing secondary metabolite accumulation. Alpinia purpurata is an ethnomedicinally important tropical monocot known to accumulate bioactive flavonoids including quercetin, myricetin, and kaempferol with established anti-inflammatory, antioxidant, and antiproliferative activities. This dataset represents the first de novo transcriptome assembly for A. purpurata and provides a foundational genomic resource for understanding methyl jasmonate-responsive flavonoid regulation in Zingiberaceae.