Identification of a new DRB1*14:54 in a french  voluntary bone marrow donor
Here we describe a novel DRB1*06 allele, identified in a french  voluntary bone marrow donor. The same Seqence was reproduced twice on two different PCR  products  by using next-generation sequencing methode (Holotype kit, Omixon, Budapest, HU) and Oxford Nanopore sequencing technology.  Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California) or by P2 Solo sequnecer (Oxford Nanopore).  Raw sequencing HLA data was analysed by Twin software (Omixon) or by NanoTYPER (Omixon) connected to the IPD-IMGT/HLA Databas.  Donor HLA genotyping was the following:   A*02:01, *33:01 B*44:03, *49:01 C*02:02, *07:01 DRB1*03:01, *14:54new DRB3*01:01,*02 :02 DQA1*01:05, *05:01 DPA1*01:03, *01:03 DPB1*02:01, *04:01 HLA-DRB1*04 :54 New allele differs from DRB1*14 :54 :01 :03  by one nucleotide substitution at position  6 of exon 4 (position  658 of cDNA), where  base C is replaced by T , inducing R instead W (CGG>TGG).