Sex Differences in BAT Thermogenesis in PGC1a Knockout Mice [ATAC-Seq]
To elucidate the sex-specific role of PGC1a in brown adipose tissue (BAT), we performed ATAC-seq analysis on BAT from male and female PGC1a knockout mice. Overall design: experiment 1: PGC-1a knockout experiment Tamoxifen-inducible adipocyte-specific PGC-1a knockout (KO) mice were generated by crossing Ppargc1a flox/flox mice with Adipoq-CreERT2 mice. Eight-week-old male and female KO and Ppargc1a flox/flox control mice were intraperitoneally injected with tamoxifen for 5 consecutive days. BAT samples were collected and analyzed at least 8 weeks after the final injection. The study included four groups based on sex and genotype: Male Control, Male KO (PGC-1a KO), Female Control, Female KO (PGC-1a KO)