OT-I T cell activation in Lrrc8af/f and Lrrc8af/f Cd4-Cre;OT-I mice
Splenocytes were separated from Lrrc8af/f and Lrrc8af/f Cd4-Cre;OT-I mice. The splenocytes were stimulated with indicated peptides (unstime/ SIINFEKL-10pM(NL)/ SIINFEKL-1000pM(NH)/ SIIGFEKL-1000nM(G4)) for 6 hours. The OT-I CD8+ T cells were labeled with Va2-PE/CD8-A700 and sorted for the sequencing. Overall design: OT-I CD8+ T cells stimulated with different peptides (unstim. /NH/NL/G4) were subjected to bulk RNA-Seq.