A framework to identify functional interactors that contribute to disrupted early retinal development in Vsx2 mutant mice II
These datasets contain the transcriptomes from E12.5 orJ-homozygous (orJ mutant) retinal tissues after organotypic culture of retina and lens for 24 hours in the presence or absence of the Retinoid-X-Receptor (RXR) antagonist HX531. The Retinoid X receptor gamma (Rxrg) gene was identified as a highly upregulated gene from the retinal RNA-Seq profile of the E12.5 orJ mutant compared to the orJ-heterozygous (control) (add in GEO accession numbers for that data). The goal of this analysis was to determine if blocking RXR activity would restore gene expression to wild type levels by comparing the retinal gene expression profiles of biological replicates from 100 nM HX531-treated and vehicle-treated (0.1% DMSO) cultures. Overall design: Comparative gene expression profiling analysis of RNA-seq data for biological replicates of E12.5 Vsx2<orJ/orj> mutant retinas treated with the Retinoid X Receptor antagonist HX531 compared to vehicle control (Dimethyl Sulfoxide).