Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [breaktag 2]
Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Characterization of the scissile profile of 4 target regions with Cas12