YAP-Driven Malignant Reprogramming of Oral Epithelial Stem Cells, ATAC sequencing
Assay for Transposase Accessible Chromatin (ATAC) sequencing of tongue epithelia-derived, transgene-selected primarily cultured cells from control mice or transgenic mice expressing HPV16E6-E7 and YAP1S127A Overall design: Fifteen days after transgene induction, cells were isolated from whole tongue epithelia, sorted for GFP expression, cultured, then subjected to ATACseq, library preparation, and sequencing on Illumina NovaSeq6000.