Single-cell RNA sequencing of developing human neural organoids exposed to an array of morphogen treatments varying morphogen concentration, timing and combinatorics
This experiment seeks to unravel the effects of concentration, timing and combinatorial variables on the response to morphogens during early human neural organoid development. Human neural organoids derived from three different hESC lines were cultured individually and exposed to systematic variations of morphogen treatments. At the end of the 21-day culture period, organoids exposed to the same treatment were pooled (this defines \"one sample\") and dissociated into single cells. Each sample was then labelled with hashing antibodies to enable multiplexing while running the Chromium?? Next?? GEM?? Single?? Cell?? 3'?? v3/3.1?? kit?? (10X?? Genomics)? workflow. Single-cell transcriptome libraries?? were?? then sequenced?? in?? SP/S1/S2?? flowcells?? with?? Illumina?? NovaSeq?? technology,?? using?? paired-end?? 28/10/10/90?? or?? 28/8/0/91?? configuration?? (Read1/IDX?? i7/IDX?? i5/Read2).