Lentiviral integration site sequencing (LIS-seq)
We propose a high-throughput technology, namely lentiviral integration site sequencing (LIS-seq), to map HIV provirus integration sites. This method has been tested on single provirus-integrated cellular clones established in this work. Single provirus-integrated cellular clones are cultured at 37 degrees under a 95 percent air and 5 percent CO2 atmosphere, in RPMI 1640 medium supplemented with 10 percent Fetal bovine serum, 1 percent penicillin-streptomycin and 1 percent GlutaMAX (100x), followed by isolation of genomic DNA that is used for the library preparation.