Sequential emergence and contraction of epithelial subtypes in the prenatal human choroid plexus revealed by a stem cell model
Despite the major roles of choroid plexus epithelial cells (CPECs) in brain homeostasis and repair, their developmental lineage and diversity remain undefined. In simplified differentiations from human pluripotent stem cells, derived CPECs (dCPECs) display canonical properties and dynamic motile multiciliated phenotypes that interacted with Aß uptake. Single dCPEC transcriptomes over time correlates well with human organoid and fetal CPECs, while pseudotemporal and cell cycle analyses highlight the direct CPEC origin from neuroepithelial cells. In addition, time series analyses define metabolic (type 1) and ciliogenic dCPECs (type 2) at early timepoints, followed by type 1 diversification into anabolic-secretory (type 1a) and catabolic-absorptive subtypes (type 1b) as type 2 cells contract. These temporal patterns were confirmed in independent derivations and mapped to prenatal stages using human tissues. In addition to defining the prenatal lineage of human CPECs, these findings suggest new dynamic models of ChP support for the developing human brain. Overall design: Four time points and two replicates per time point were harvested. Following single cell dissociation, derived CPEC cultures were FACS sorted for live single cells and subjected to 10x Chromium capture for ~10,000 cell per sample and Illumina sequencing for ~50,000 reads/cell. Datasets were processed with SoptSC.