PD-1 regulates ILC3-driven intestinal immunity and homeostasis
PD-1 is constitutively expressed on a fraction of ILC3 in the gut. To identify the transcriptional differences between PD1+ and PD1- ILC3, we have sorted these subsets and analysed their transcriptional regulation by RNA-Seq. Overall design: PD1 expressing and PD1 negative ILC3 from the small intestine of adult female rorgt-reporter mice. ILC3 NCR+ were excluding, ILC3 were sorted as CD45+Lineage- (CD3, CD11b, B220, TER119, TCRb, CD19, NK1.1, F4/80) CD90.2+ROR?t+ NCR-