Continuous in vitro culture of Cryptosporidium parvum
Cryptosporidium parvum (isolate BGF-T0) was cultured in a hollow fiber bioreactor as described in Morada et al., 2016, Int J Parasitol 46:21-29. At 20 months 108 oocysts were collected and assigned isolate BGF-T20HF. Genome sequences were generated at the Beijing Genomics Institute using 1 ug of purified DNA and compared with the parent BGF-T0.