GenomePAM directs PAM characterization and engineering of CRISPR-Cas nucleases using mammalian genome repeats
We developed a method named GenomePAM that allows for direct PAM characterization in mammalian cells.Beyond PAM characterization, GenomePAM allows for simultaneous comparison of activities and fidelities among different Cas nucleases on thousands of match and mismatch sites across the genome using a single gRNA, and provides insight into the genome-wide chromatin accessibility profiles in different cell types.