The sequencing of 16S rRNA genes of bacteria from soil samples of pseudo-rhizosphere device.
Three different field soils were used to analyze the bacterial communities of tomato roots and bulk soils.  Field soils were collected from three sites in Japan: Kyoto University (Kyoto), Tokyo University of Agriculture and Technology (Fuchu), and Kyoto University of Advanced Sciences (Kameoka). Soil from each field was mixed with the sterilized Toyoura sand in a volume ratio of 1:9. Tomatoes were cultivated for 3 weeks. Tomato roots and bulk soils were sampled, and DNA was extracted. The V4 region of 16S rRNA genes was amplified using PCR.