Pseudomonas sp. FA-1 Genome sequencing
Whole genome sequencing of a tick-associated Pseudomonas sp. isolated from Hyalomma dromedarii ticks collected from camels at Hail Province, Saudi Arabia. Average nucleotide identity analysis (87.5% to the closest relative, P. guariconensis type strain LMG 27394) and digital DNA-DNA hybridization (dDDH 32.9%) indicate this isolate represents a potentially novel genomospecies within the P. putida group. The genome was assembled using SPAdes from Illumina paired-end reads, yielding a 5.1 Mb draft genome (38 contigs at 500 bp or greater, N50 363 kb, GC 63.75%). Annotation by Prokka identified 4,541 protein-coding genes, 77 tRNAs, and 5 rRNAs. AMR screening detected only intrinsic efflux pump genes (MexAB-OprM, MexCD-OprJ, MexEF-OprN) with no acquired resistance determinants. This project supports characterization of tick-associated bacteria and antimicrobial resistance surveillance in arthropod vectors from Saudi Arabia.