miRNA-Seq analysis in cotyledons and hypocotyls of elo3-6 mutant (lacking functional Elongator complex)
The goal of this project is to study the miRNAs expression in cotyledons and hypocotyls of 4-day old Elongator mutant (elo3-6) and wild-type (Col-0) Arabidopsis seedlings during photomorphogenesis. elo3-6 plants carries a mutation in the ELP3 gene encoding catalytic subunit of the Elongator complex. This complex regulates gene expression during transcription and translation via epigenetic and tRNA modifications, respectively, and is associated with the post-transcriptional regulation of gene expression mediated by miRNA. Seedlings of elo3-6 show photomorphogenesis defect known as light hyposensitivity (elongated hypocotyls, reduced angle between cotyledons and hyponastic growth of the cotyledons).