Transcriptional profiling of mature murine adipocytes of the inguinal white adipose tissue depot following LPCAT3 deletion and HFD-induced adipose hypertrophy.
To analyze the contribution of dietary PUFAs and adipocyte LPCAT3 expression in modulating the transcriptome of murine inguinal white adipose tissue during adipocyte hypertrophy. We performed bulk RNA sequencing with a two-factor experimental design. WT and AdL3KO mice were fed an omega-6 enriched high-fat diet (60% Calories from fat) for 0 weeks (standard chow from weaning), 1 week, 5 weeks, or 10 weeks Overall design: Comparative gene expression profiling analysis of iWAT from WT and AdL3KO mice on an omega-6 enriched high-fat diet (60% Calories from fat) for 0 weeks (standard chow from weaning), 1 week, 5 weeks, or 10 weeks