Transcriptome of 3D cultivated colon cancer cell lines (HCT116, SW620, DLD1) and HCEC-1CT
The study aimed to identify transcripts of the protein-coding genes that could have regulatory role potentially relevant for malignant transformation of the gut mucosa and colon cancer onset and progression. The set of colon cancer cell lines (HCT116, SW620 and DLD1) and immortalized cells derived from healthy gut mucosa (HCEC-1CT) were cultivated in 3D and their transcriptomes were analyzed by RNA sequencing to profile expression for both coding and non-coding transcripts. Overall design: We performed transcript expression profiling of 3 colorectal cancer cell lines and HCEC-1CT cell line cultivated in 3D using RNA sequencing.