MAF Amplification licenses Estrogen Receptor a to Drive Breast Cancer Metastasis.[ChIP-Seq]
We performed genome-wide mapping of H3k27ac and H3K4me3 sites in control and MAF-overexpressing MCF7 cells to assess the consequences of estrogen (E2) stimulation and MAF overexpression recruitment to chromatin. To this end, we cultured MCF7 cells in hormone-deprived (HD) medium for 72 h and then E2 or vehicle was added for 1h prior to chromatin immunoprecipitation (ChIP). Samples were generated in triplicate. Overall design: Examination of H3K27ac and H3K4me3 sites in control and MAF-overexpressing MCF7 cells before and after E2 stimulation.