Multi-kingdom rumen microbiome profiling in dairy calves at weaning and post-weaning
This project characterizes the rumen microbiome of dairy calves during the transition from weaning to post-weaning. Amplicon sequencing was performed to profile bacterial and archaeal communities using 16S rRNA gene primers targeting the V3-V4 region, anaerobic fungi using primers targeting the D2 region of the 28S rRNA gene, and protozoa using 18S rRNA gene primers targeting the V3-V4 region.Rumen samples were collected longitudinally at weaning and post-weaning timepoints. The study aims to investigate the establishment and dynamics of multi-kingdom microbial communities in the developing rumen and their potential implications for host digestion and performance.