Promoter and NLS comparisons for Cas9 in Arabidopsis thaliana
We have compared the efficiency of several promoters to express Cas9 and several NLS configurations for Cas9 at several targets in simplex and multiplex. To do this we transformed Arabidopsis thaliana (Col-0) plants with our different CRISPR/Cas9 constructs. In the T2 generation, we genotyped thousands of seedlings at up to seven target sites using Illumina amplicon sequencing.