Dynamics and regulatory roles of RNA m6A methylation in unbalanced genomes
N6-methyladenosine (m6A) in eukaryotic RNA is critical for RNA metabolism, gene expression regulation, and the development of organisms. Aberrant expression of m6A components appears in a variety of human diseases. RNA m6A modification in Drosophila has proven to be involved in sex determination regulated by Sxl and may affect X chromosome expression through the MSL complex. The dosage-related effects under the condition of genomic imbalance (i.e., aneuploidy) are related to various epigenetic regulatory mechanisms. We investigated the roles of RNA m6A modification in unbalanced genomes using aneuploid Drosophila. Overall design: m6A methylated RNA immunoprecipitation sequencing (MeRIP-Seq) for wildtype female, wildtype male, trisomy 2L female, trisomy 2L male, and metafemale Drosophila.