Single-cell RNA sequencing reveals the impact of CD34+ cells during heart failure in the hypertrophic heart with hyperlipidemia
Hyperlipidemia and hypertension might play a role in cardiac fibrosis, in which a heterogeneous population of fibroblasts seems important. However, it is unknown whether CD34+ cells are involved in the pathogenesis of cardiac fibrosis. This study aimed to investigate the mechanism of CD34+ cell differentiation in cardiac fibrosis during hyperlipidemia. By analyzing the transcriptomes of single cells from the Cd34-CreERT2;R26-tdTomato&ApoE-/- heart, we also demonstrated the dynamics of cell landscape during cardiac fibrosis in the hypertrophic heart with hyperlipidemia. Overall design: scRNA-seq for total non-cardiomyocytes and CD34+ cells from Cd34-CreERT2;R26-tdTomato&ApoE-/- mice hearts