mRNA-Seq of Danio rerio exposed to different concentrations of fungicide epoxiconazole against untreated control groups
In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to epoxiconazole (CAS 133855-98-8). Zebrafish embryos were exposed to epoxiconazole according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 system System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential substance specific biomarker candidates were selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.