lung cancer patients tumor tissues and 3D cancer cell RNA sequence
To investigate the impact of co-culturing CAFs and HUVECs on the transcriptomic characteristics of lung cancer cells, we processed primary lung cancer cells from seven paired patients (LC-11, LC-13, LC-14, LC-18, LC-19, LC-21, and LC-22) and categorized them into five groups: monoculture (2D-LC, Mono-LC) and co-culture (3D-2-LC_1, 3D-2-LC_2, and 3D-3-LC).After seven days of incubation, the microbeads were dissociated, and the lower chamber cells were harvested for RNA extraction and transcriptome sequencing.