ChIP-seq of the new histone H4 variant of Trypanosoma cruzi
In this study, we identified a H4.V in T. cruzi that is encoded by a single-copy gene located apart from the typical tandem arrays of canonical histone H4. Functional characterization using ChIP-seq assays revealed that H4.V is located at subtelomeric regions, demarcates convergent strand-switch regions (cSSRs), and determines potential new transcription termination sites at codirectional PTUs interrupted by tDNA loci.