RNA-sequencing of Arabidopsis rrp1-3 mutants, RRP1-overexpressing line and wild type plants
Abscisic acid (ABA) plays important roles in many aspects of plant growth and development, as well as various stress adaptation. Although much progress has been made toward understanding the molecular mechanisms for ABA homoeostasis and signaling, how plant cells integrate ABA trafficking and signaling to regulate plant developmental processes is poorly understood. Manipulation of AtRRP1 expression affects ABA sensitivity in seed germination and seedling growth, as well as several ABA transporter gene transcripts and ABA contents. To overall understand Arabidopsis RRP1-regulated genes in the transgenic plants, three groups including the rrp1-3 mutants (homozygote gained by CRISPR) and the RRP1-overexpressing line (Super: RRP1-3) and the wild type plants (WT) was used for RNA-sequencing (RNA-seq).