Microalgal VTC protein sequencing and modelling
Protein models were generated for the VTC4 protein of Chlamydomonas reinhardtii and putative VTC4 homologues in Chlorella vulgaris, Desmodesmus cf. armatus, and Gonium pectorale. RNA sequencing, transcriptome assembly, and protein prediction were used to identify the putative protein sequences of C. vulgaris and D. armatus, while the sequence for G. pectorale was found through a search of NCBI. Biochemical assays were used to compare polyphosphate synthesis kinetics between the algae.