dCAPS Marker Linked to Rf Gene in Pepper
This dataset originates from a study conducted to identify molecular markers linked to the restorer-of-fertility (Rf) gene in pepper (Capsicum annuum) associated with the cytoplasmic–genic male sterility (CMS) system. An F2 population was developed from a cross between the non-restorer line IHR4392 (rfrf) and restorer line IHR4597 (RfRf), and phenotypic evaluation confirmed monogenic dominant inheritance of fertility restoration. Genotyping-by-sequencing (GBS) generated 10,443 high-quality SNPs, enabling bulk segregant analysis (BSA). Three SNPs co-segregating with the Rf locus were identified on chromosome 6; one SNP at 2,343,509 bp was converted into a derived cleaved amplified polymorphic sequence (dCAPS) marker and mapped 7.90 cM from the Rf gene. Candidate genes identified in this region include two fertility restorer-like genes (CA06g01070, CA06g01090) and a pentatricopeptide repeat protein (CA06g01100). This submission supports marker-assisted selection for stable CMS-based hybrid breeding in pepper.