Identification of the alternative sigma factor regulons of Chlamydia trachomatis using multiplexed CRISPR interference
RNAseq analysis was performed for C. trachomatis serovar L2 after knocking down or overexpressing different sigma factors. Overall design: RNA was collected from C. trachomatis L2 infected cells following 14 hours of sigma factor knockdown or 4 hours of sigma factor overexpression. Each strain was tested in triplicate, resulting in 48 total samples. Induced knockdown samples were compared to their respective uninduced samples as well as an induced non-targeting knockdown sample. Overexpression samples were compared to their respective uninduced samples.