The profiling of methylation and acetylation modification of histone H3 in Intrahepatic cholangiocarcinoma cells
Abnormal distribution of histone modifications has been confirmed to influence the development and progression of neoplastic diseases. Methylation modifications of histone H3 include H3K9me3, H3K27me3 and H3K4me3. Acetylation modifications of histone H3 include H3K9ac and H3K27ac. Among these modifications in the promoter region, H3K9me3 and H3K27me3 serve as inhibiting factors for transcription, while H3K4me3, H3K9ac and H3K27ac are important promoting factors for transcription. To make sure the distribution of these histone modifications in intrahepatic cholangiocarcinoma (ICCA) cells, CUT & Tag was performed. We found that these modifications were located in the promoter or non-promoter regions (e.g., intergenic, exon and intron regions) of key tumor-associated genes, indicating the potential role of chromatin remodeling complexes in ICCA diagnosis and treatment. Overall design: This dataset includes six CUT & Tag samples. Immunoprecipitation was performed with antibodies for different histone modifications (H3K9me3, H3K9ac, H3K27me3, H3K27ac and H3K4me3). The immunoprecipitation of control group was performed by anti-IgG antibodies.