Effect of K14Q MOTS-c treatment on cast immobilization-induced skeletal muscle atrophy
To understand the effect of K14Q MOTS-c treatment on cast immobilization-induced skeletal muscle atrophy, we injected K14Q MOTS-c into casted mice. Overall design: Male C57BL/6J mice at 10 wk of age were obtained and assigned to one of the immobilization and K14Q MOTS-c treated group (15 mg/kg/day MOTS-c injection for 8 days, twice a day intraperitoneal injection, n = 8). Hindlimb immobilization was performed by using casting and the casting material was attached to both hinds. After eight days of hindlimb immobilization, mice were euthanized and gastrocnemius muscle was collected under anesthesia with isoflurane for RNA-seq.We then performed gene expression profiling analysis using data obtained from RNA-seq. The RNA-seq data in non-iimobilization control, immobilization control, and immobilization and WT MOTS-c treatment are depositted in GSE212709.