Propionate resistance in Y. enterocolitica
The goal of this study was to understand mechanisms of propionate toxicity in Y. enterocolitica. Y. enterocolitica was grown in LB broth statically overnight at room temperature in increasing concentrations of propionate, starting at 5 mM propionate, and ending with 20 mM. Single colonies of eight evolved cultures were isolated and purified. WGS was performed to detect SNPs that allow for growth in the presence of otherwise lethal concentrations of propionate. As a control, the wild-type strain (starting strain) was sequenced as well.