Effect of EPCR knockdown by siRNA in human umbilical vein endothelial cells
Endothelial protein C receptor (EPCR, PROCR) is a single-pass transmembrane glycoprotein that is detected on the surface of vascular endothelial cells (ECs) and stem cells. Besides the anti-coagulation role, EPCR can mediate intracellular signaling of activated protein C (aPC)[7]. The activation of downstream protein kinase B (AKT) promotes cell growth, improves cardiac function, and favors tumor progression. However, the role of EPCR in pathological retinal neovascularization remains unclear.To investigate the role of EPCR in endothelial cell function, we knockdown EPCR with siRNA and compared with siCtrl. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different samples each condition. Overall design: 8 samples were analyzed: siCrt (4 replicates), siEPCR (4 replicates)