Therapeutic effects of medicinal and food-based traditional herbal couples on type 2 diabetes mellitus based on pharmacodynamics and pharmacokinetics
16s rDNA sequencing was used to detect changes in the gut flora of rats. The 16s rDNA gene is the DNA sequence corresponding to the ribosomal RNA encoded by bacteria, which contains 9 highly variable regions and 10 conserved regions. 16s rDNA reflects species differences and conserved regions reflect species affinity. The highly variable regions reflect interspecies differences and the conserved regions reflect interspecies affinities, and are found in the genomes of prokaryotes. 16s rDNA has a high degree of specificity, conservatism and stability. The mutation rate is low, which is suitable for microbial phylogeny and identification of indicators. 16s rRNA coding sequence is 16s rDNA, but in the experiment RNA extraction is relatively difficult, while DNA extraction is relatively easy, so this experiment on the 16s rDNA sequencing analysis. The basic principle of 16s sequencing is PCR amplification. 16s rDNA gene detection technology has become a powerful tool for microbial detection and identification.