Metabolic regulation of Th2 cells by L-phenylalanine
RNA-Seq of in vitro differentiated Th2 cells subjected to L-phenylalanine treatment Overall design: In vitro long differentiated Th2 cells from 5 healthy donors were incubated in RPMI+10%Fetal Calf Serum +100IU recombinant human Interleukin 2 with Vehicle or 1mM additional L-Phenylalanine with or without simultaneous activation with anti-CD2, anti-CD3 and anti-CD28 antibody beads (1:1 beads:cells).