miRNA Profiling of Extracellular Vesicles from Mouse Inner-Ear Organoids Across Proliferation and Differentiation Stages
This project provides small-RNA sequencing data that capture developmental changes in the miRNA cargo of extracellular vesicles (EVs) released by mouse inner-ear (cochlear) organoids. Organoids were generated from post-natal day-2 mouse cochlear duct cells, expanded for seven days in proliferation medium, then switched to a 20% Matrigel/differentiation protocol for an additional 14 days. Culture media from proliferating spheres (days 5 to 7) and differentiated organoids (days 19 to 21) were collected, and EVs were purified using a two-phase aqueous isolation kit. Total EV RNA was extracted, libraries were prepared with the SMARTer smRNA-Seq kit and sequenced on an Illumina NovaSeq platform (single-end 75 bp).