Gene expression effects of donepezil in human primary cells
The goal of this project is to evaluate the acetylcholinesterase inhibitor donepezil as a topical treatment for inflammatory skin disorders. Primary adult human epidermal keratinocytes (Gibco/Thermo Fisher Scientific) were treated for 1 day with 100 uM donepezil followed by extraction of total RNA with the RNeasy Plus mini kit (QIAGEN). In a second experiment, human primary CD4+ T cells obtained by leukapheresis from 3 donors was treated for 3 days with 50 uM donepezil followed by RNA extraction.