Metabarcoding in the Thau and Berre lagoons to detect Veneroidaee
Seawater was filtered in June-July 2023 in Thau and Berre lagoons. The filtering capsules (24) are Waterra systems using a customized pumping system commercialized by Argaly company. DNA was resuspended in the capsule vigorous agitation after the injection of Longmire buffer, and extracted at Argaly. Whole DNA was used to construct libraries of metabarcoding using 16S primers specific to Veneroidae.  After purification, 8 PCR replicates of each sample were submitted to NGS sequencing and subsequently merged. Besides an experiment was conducted in October 2022 in Ifremer Sete and 14 additionnal capsules were obtained from the filtering of water in aquariums filled with different ratio of two clam species.