Transcriptome analysis of Ngb-Knockout in Zebrafish (Danio rerio)
To investigate the function of vertebrate neuroglobin (Ngb) in vivo we generated a ngb-deficient zebrafish using CRISPR/Cas9 methodology. Hypoxia and temperature were employed to investigate the in vivo function of neuroglobin. Neuroglobin deficient animals exhibited reduced hypoxia tolerance and increased expression of known oxygen-binding and glycolysis-related transcripts. RNA-Seq analysis revealed that a moderate temperature-drop activated a distinct molecular response.