Sequencing of Saccharomyces cerevisiae strains with tandem amplified GFP and Aeblue genes
We aimed to link cellular fitness to production of a desirable compound in model organism Saccharomyces cerevisiae. A tri-vector system was used to integrate a bacterial mRNA interferase toxin RelE, along with its orthogonal antidote RelB in sequence with the productive gene. Induction of the toxin increases the selection pressure for high antitoxin producing cells, one outcome of which is the recovery of a strain with a high copy number of tandem-repeated antitoxin-gene of interest arrays. The sequences contained within this submission were obtained from yeast produced by this method and were sequenced in the course of its characterisation.