Alternative polyadenylation contributes to the Resistance of the Jiangxi hybrid rice to blast rice using transcriptomics and metabolomics
Rice (Oryza sativa) is one of the world's major rations. The widely planted hybrid rice in Jiangxi Province is seriously affected by Magnaporthe oryzae, which seriously restricts the rice yield in Jiangxi Province. In recent years, studies have found that alternative polyadenylation (APA) is an important post-transcriptional regulation in eukaryotes. The genetic law and function of APA in the occurrence of hybrid rice blast in Jiangxi are not clear. In this study, Wufengyou T025 (WFY) and its parents (male parent Changhui T025(CH), and female parent Wufeng B(WFB)) were used as the research objects. Transcriptome and metabolome sequencing, combined with a variety of bioinformatics analysis methods and modern molecular biology experimental techniques, revealed the APA expression profile during the occurrence of Jiangxi hybrid rice blast. To explore the regulatory mechanism of APA core factors during the occurrence of rice blast. This study not only provides a new understanding of the occurrence of Jiangxi hybrid rice blast, but also provides more abundant resources for molecular breeding. Overall design: Rice (Oryza sativa L.) varieties were the hybrid Wufengyou T025 (WFY) along with its male line Changhui T025 (CH) and female line Wufeng B (WFB). The transgenic lines of wd-1 were generated by wild type (WT) Nipponbare plants. Appropriate amount of rice leaves was taken at 0 hours, 12 hours, 24 hours, 36 hours, and 48 hours, respectively.