Differential gene expression in IFNg+ and IFNg- IL-15 primed NK cells after anti-NK1.1 stimulation [IFNgReporter_RNA-seq]
We isolated splenic NK cells and cultured them for 3 days in 100 ng/ml IL-15. Next, we stimulated the cells with plate-bound anti-NK1.1 for 6 hours and sorted YFP+ (IFNg+) and YFP- (IFNg-) populations. We then performed gene expression profiling analysis using data obtained from RNA-seq from unstimulated, YFP+ or YFP- NK cells. Overall design: RNA-seq