RNA-seq and Ribo-seq of PHYHD1-WT,PHYHD1?107-130(MT) and EV ccRCC cell line(7860)
We used RNA seq and Ribo-seq to evaluate the PHYHD1 gene expression changes among different groups (EV, MT, WT) to investigate the potential mechanisms by which PHYHD1 exerts its negative regulatory role in inhibiting excessive protein synthesis. Overall design: The 7860 ccRCC kidney cancer cell lines, carrying empty vector, overexpressing PHYHD1 (MT), and the untreated wild-type group, were cultured in vitro in three separate 10 cm dishes for each condition, and the samples were subsequently collected for experiments.