16S rRNA gene amplicon sequencing of Capsicum annuum L. rhizosphere soil under Scotellopsis reticulata treatment
This study evaluated the effect of the soil-borne microalga Scotellopsis reticulata on the rhizosphere bacterial community of Capsicum annuum L. using 16S rRNA gene amplicon sequencing. Rhizosphere soil samples were collected from Capsicum annuum L. grown in pots under controlled conditions. Two treatments were included: control (no microalgae) and S. reticulata treatment (applied at 1x10^7 cells/mL on days 1, 15, and 30 after transplantation). After 45 days of growth, rhizosphere soil was collected from five biological replicates per treatment (each replicate pooled from two pots). Total DNA was extracted and the V3-V4 region of the 16S rRNA gene was amplified using primers 27F-1492R. Amplicons were sequenced on the PacBio Sequel II platform, generating high-fidelity reads. Raw sequence data are provided in FASTQ format. These data are intended for analysis of bacterial community composition, diversity, and co-occurrence networks in response to microalgal inoculation.