Global iCLIP in naive and primed mouse embryonic stem cells to analyse RNA-binding protein occupancy on RNA
Global iCLIP was performed on UV-crosslinked mouse naive embryonic pluripotent stem cells (nPSCs) and primed pluripotent stem cells (pPSCs) (150 mJ/cm² at 254 nm) using the iiCLIP protocol (https://doi.org/10.1101/2021.08.27.457890 ), omitting the immunoprecipitation step to capture all crosslinking events.