CUT&Tag profiling of JUND chromatin binding in human mast cell line LAD2 under SCF stimulation and imatinib perturbation
Mast cells respond to cytokine cues through rapid transcriptional rewiring. Here we generated CUT&Tag profiles of the transcription factor JUND in the human mast cell line LAD2 under three culture conditions: SCF withdrawal (EM), SCF supplementation (SCF), and SCF withdrawal plus imatinib treatment (IM). Two biological replicates were collected for each condition. Libraries were sequenced as paired-end reads and processed by adapter/quality filtering, alignment to the human reference genome (GRCh38/hg38), peak calling, and generation of normalized genome browser tracks. This dataset provides a resource to examine how SCF signaling and c-KIT pathway inhibition reshape JUND occupancy in mast cells. Overall design: CUT&Tag (TF binding) profiling in LAD2 cells targeting JUND (Active Motif 61403; RRID:AB_2793621). Three treatment conditions with two biological replicates each: SCF- (EM), SCF+ (SCF), and SCF- + imatinib (IM). Paired-end sequencing; genome: GRCh38/hg38. No IgG/input/no-antibody control library was generated.