Metabarcoding with amplicon sequencing of microalgae in the gut and breeding sites of Aedes aegypti larvae in Thailand
The objectives of this study were (1) to survey microalgae in the gut and breeding sites of Ae. aegypti larvae in Nakhon Si Thammarat, Thailand, using light microscopy and metabarcoding with amplicon sequencing; (2) to investigate the species composition, relative abundance, and relationship of microalgae in the gut of Ae. aegypti larvae and in their breeding sites; and (3) to investigate the diversity of the microalgal communities living in the gut and breeding sites.