The transcriptome of the parapoxvirus Orf virus reveals novel promoters for heterologous gene expression by poxvirus vectors
RNA-sequencing (RNA-Seq) was used to analyze the transcriptome of parapoxvirus orf virus (ORFV) during infection in primary ovine turbinate cells. Based on the transcriptional profile of individual ORFV genes, we identified ORFV promoters with strong early and late activity and have shown that they can be used to express heterologous genes in ORFV vectors.