de-novo sequencing of Lilium longiflorum pollen transcriptome
To identify proteins and enzymes in lily pollen, total RNA was isolted from pollen of Lilium longiflorum Thunb. at various states during germination ranging from 0 to 120 min in germination medium. mRNA was purified from the pooled RNA preparations, transcribed to cDNA and a normalized cDNA library was sequenced (Illumina HiSeq2000, BGI Genomics)