RNA-seq of mice islets from wt and miR-29a/b-1 pancreatic beta cell specific overexpression transgenic mice
We investigated the mechanism associated with miR-29 effects by comparing gene expression of whole primary islets isolated from miR-29a/b-1 pancreatic beta cell specific overexpression transgenic mice (ßTG mice) and their control littermates by RNA sequencing. Ten-week-old mice were chosen to avoid secondarily affected genes. We identified 346 genes that showed significant differences (p-value < 0.05 and >2-fold change) between the two groups. We then used gene ontology (GO) algorithms to perform functional annotations of these differential genes. The top enriched pathways were linked primarily to inflammatory response, secretory vesicle, and ion channel activity. Islets from the ßTG mice showed an enhanced inflammatory response based on an assessment of both the upregulated and the downregulated genes , which suggested a likely positive regulation of phagocytosis (Ahsg), regulation of blood vessel diameter (P2rx1/Kng1/Nos2), and monocyte chemotaxis (Cxcl10/Cxcl17). Overall design: islets mRNA of wt and miR-29a/b-1 pancreatic beta cell specific overexpression transgenic mice