Amplicon sequencing of nitrogen-fixation gene (nifH) from soil samples in Horqin Sandy Land
These sequences represent the nifH gene (encoding nitrogenase iron protein) amplified from soil samples collected under sand-fixation plantations in the Horqin Sandy Land, Inner Mongolia, China. Samples were taken along a herbaceous vegetation successional gradient (shifting sand, early, middle, and late successional stages). The nifH gene fragments (approximately 370 bp) were amplified using primers PolF and PolR and sequenced on the Illumina MiSeq platform. This study aimed to investigate the response of diazotrophic community abundance, diversity, and structure to vegetation succession. The sequences provide insight into the diversity of nitrogen-fixing bacteria during ecological restoration of sandy soils.