GUIDE-seq profiling of the Cas9 off-targets for PRF1, HBB, UNC13D gene editing
Primary T cells of FHL2 and FHL3 patients were treated with Cas9 nuclease mixed with a single (UNC13D) or two sgRNAs (PRF1, HBB), and profiled with GUIDE-seq according to the protocol published in Tsai et al. Nat Biotech 2015.