N6 methyladenoside sequencing of T cells
N6 methyladenoside (m6A) sequencing was performed on induced Treg cells, Tnaive cells, CD4+ T cells from germ-free mice and CD4+ T cells from specific pathogen free mice. We found that there are 465 genes with increased m6A levels and 4083 genes with decreased m6A levels in iTregs compared to Tnaïve cells. We found that there are 291 genes with increased m6A levels and 344 genes with decreased m6A levels in germ-free CD4+ T-cells compared to T cells from specific pathogen free mice Overall design: mRNA profiles of induced Treg, Tnaive, and CD4 T cells from germ-free mice and specific-pathogen-free mice.