PFOS induced methylome alterations in human breast cell line
The dataset is composed Perflourooctanesulfonic acid (PFOS) exposed normal human breast cells (MCF-10A). Cells were exposed for 72h to 1µM PFOS, control were exposed to 0.1% DMSO, 6 samples per group. DNA methylation levels were assessed with Ezymatic Methyl sequencing (EM-seq), pipeline similar to BS-seq. The aim of the study is to elucidate methylation alteration induced by PFOS exposure. Differentially methylated regions (DMRs) are sought after, overlapped with genomic features (promoters, exon, CpG-islands) and the identified genes are gene ontology analysed. From previous studies, PFOS has shown to induce malignant phenotypes to MCF-10A, such as increased growth and locomotion. These phenotypes are also sought after.