Bacterial Protoplast-Derived Nanovesicles Carrying CRISPR Cas9 Tools Efficiently Re-educate Tumor-Associated Macrophages for Cancer Immunotherapy
It demonstrated the obvious cleavage of Pik3cg after T7E1 digestion and the indel rate was about 28.5%.NGS were also performed to evaluate the in vivo gene-editing efficiency of sgPik3cg DHP/DGA-NVs and confirmed successful Pik3cg gene-editing in TAMs by sgPik3cg-DHP/DGA-NVs.