Revealing the Immune Response of Sitona callosus to Beauveria bassiana Infection through Integrative Analyses of Transcriptomics and Metabolomics
The legume pest Sitona callosus poses a significant threat to alfalfa growth. Due to its unique life cycle, Beauveria bassiana has emerged as an effective fungus for controlling S. callosus . We conducted a comprehensive analysis of the transcriptome and metabolome of S. callosus infected by B. bassiana. Differential expression of antifungal genes, including heat shock genes, cytochrome P450 (CYP450) genes, cathepsin proteases, and C-type lectin were identified in S. callosus. Pathway analysis revealed associations between immune genes and metabolites involved in autophagy-animal, glucagon signaling pathway, and glycerophospholipid metabolism. These findings provide valuable insights for enhancing the control efficacy of Beauveria bassiana on Sitona callosus. Overall design: In order to determine the immune response of Sitona callosus infected by Beauveria bassiana. Transcriptomic tests were performed on Sitona callosus after infection with Beauversiana bassiana and the uninfected one.