Genetic Dissection of Photosynthetic Organs Contribution to Seed Oil Accumulation in Arabidopsis seeds
We report the application of RNA-seq technology for highthroughput profiling of photosynthetic and non-photosynthetic seeds of Arabidopsis chlorophyll synthase mutant seeds. By generating over 21 GB of sequence data from these seeds, we showed that genes involved in oil accumulation in non-photosynthetic seeds were significantly induced compared to photosynthtic seeds. Additionally we found that genes involved in the plastidal oxidative pentos phosphate pathway were significantly upregulated in the non-photosynthetic seed opposite to photosynthetic seeds. Overall our RNA-seq analysis revealled the genes and pathway interaction underpinining oil accumulation in non-photosynthetic seeds. Overall design: Examination of segregating green (photosynthetically-competent) and non-green (photosynthetically-non-competent) seeds in siliques of an Arabidopsis chlorophyll synthase mutant.