A new B*13 allele
Here we describe a novel HLA-B*13  allele, identified in a a french  volunteer bone marrow donor. The same Seqence was reproduced twice on two different PCR  products by using two different next-generation sequencing (NGS) reagents (Holotype kit, Omixon, Budapest, HU and NG-MIX kit, UPR, RENNES , France) . Each  of the two ADN libraries was loaded onto the MiSeq platform (Illumina, San Diego, California). Raw sequencing HLA data was analysed by TypeStream™ Visual NGS Analysis Software (TSV EFS) provided by ONE LAMBDA or by Twin software (Omixon) connected to the IPD-IMGT/HLA Databas. Donor HLA genotyping was the following:  A*02:01 ,*24:02 B*13:New , B*15:01 C*03:03 ,*06:02 DRB1*01:01 , *07:01 DRB4*01:03  DQA1*01:01 , *02:01 DQB1*02:02 ,*05:01 DPA1*01:03 ,*01:03 DPB1*04:01 ,*- HLA-B*13 : New allele differs from B*13 :59  by one nucleotide substitution at position 69  of exon 3 (Posion 412 of cDNA) , where  base A  is replaced by inducing Asp instead Asn at codon 114th .