DREB-DAP-SEQ
C. indicum genomic DNA was fragmented into 100-400 bp using the Bioruptor Plus. The fragmented DNA was end-repaired, followed by the addition of a 3 A-tail, and adaptor ligation. Recombinant CmDREBa was expressed as a fusion protein containing the HaloTag sequence and subsequently purified using HaloTag ligand-conjugated magnetic beads (Promega, USA). The genomic DNA library was incubated with the Halo-CmDREBa protein at room temperature for 1 h. DNA specifically bound to the Halo-CmDREBa protein was then eluted, recovered, and amplified by PCR.