The maize (Zea mays L.) inbred lines Chang7-2 and Mo17 were grown in growth chambers (28 oC, 16 h day and 22 oC, 8 h night cycles).
The crude extracts of maize leaves tissue infected with SCMV were prepared by mixing 0.01M phosphate buffer solution (0.01 M KH2PO4: 0.01 M Na2HPO4 = 49: 51 (V/V), pH 7.0) at a ratio of 1:10 (W/V). The crude extracts were inoculated on the second leaves of six-day-old Chang7-2 and Mo17 maize seedlings, and buffer-inoculated plants were used as control.