Effect of depletion of RNH1 on gene expression in KYSE510 cell
Accumulation of R-loops causes DNA breaks and genome instability, as well as regulates tumorigenesis in a mechanism that remains poorly understood. We knockdown the ribonuclease RNase H1 (RNH1) to generate a cell model of induced R-loop accumulation. We showed that the expression of a large number of genes was disturbed after RNH1 knockdown, including 961 genes with more than twofold differences . The KEGG pathway indicates that the target genes are enriched in the Hippo signaling pathway, Wnt signaling pathway, and other tumor-associated biological processes . Overall design: To establish a novel induced R-loop model, we knocked down the ribonuclease RNH1 in KYSE510 cells with shRNA.