MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer [RNA-seq]
The effect of gene perturbation on the transcriptome was evaluated in human Cas9 expressing cancer cell lines Overall design: Human pancreatic cancer cell lines were cultured in DMEM,RPMI or HITES  supplemented with 5 or 10% fetal bovine serum (FBS). RNA-extraction (Triazol) and Poly-A followed by library preparation was performed in accordance to manufacturer's instructions (Illumina Truseq Kit).