RNA-seq of Bordetella pertussis WT BPSM and crpH knockout strains cultivated in high and low aeration
This experiment was designed to compare the transcriptome of a KO mutant of Bordetella pertussis (inactivation of bp2921,that we have called crpH) with that of the wt strain, BPSM. Because we observed that growth of the mutant was affected in low-aeration conditions, we grew wt and mutant bacteria with or without agitation (200 rpm) in SS medium supplemented with 20 mM MgSO4 (the latter was added to maximize expression of the operon containing the gene of interest).