Next-generation RNA sequencing of human astrocyte cultures to study the comparative levels of transmembrane proteins and their transcriptional variants
The aim of this study was to perform Next-generation RNA sequencing to analyse the expression of transmembrane proteins in human astrocytes and compare the prevalance of transcriptional variants thereof. Overall design: Human astrocyte cell cultures were allowed to grow to 70% confluence in DMEM supplemented with 10% FBS and 1% antibiotics. RNA was isolated from the cultured cells and processed to prepare cDNA libraries compatible with Illumina HiSeq NEXT Generation high-throughput sequencer. After the 2 x 100bp Illumina HiSeq paired-end sequencing run was complete, data in the form of BCL files was procured and processed to obtain relevant findings.