ChIP-seq data from induction experiments with pZEVi-LYS2::boxB yeast
Paired-end sequencing of sheared genomic DNA fragments originating from the sonicated chromatin of formalin-crosslinked yeast samples, purified from each sample after chromatin immunoprecipitation (IP) and in parallel without pull-down (input). IPs were carried out with anti-HA antibody for HA-tagged Cas9, Cas9-2xN, dCas9, or dCas9-2xN variants of SpCas9-NG. Yeast cultures were treated with Beta-Estradiol (15nM or 1000nM) to induce pZEVi or left untreated (0nM) during growth at 30C in SC-Leu-Ura media that maintains Cas9/sgRNA episomes. The Cas9(-2xN) strains had a non-targeting sgRNA and the dCas9(-2xN) strains had a targeting sgRNA.