CAGE-seq of day 0 (D0) and day 7 (D7) 3T3-L1 cells before and after adipogenic treatment
3T3-L1 preadipocytes were differentiated into mature adipocytes using adipogenic differentiation media. Total RNA was isolated at D0 (two days post confluency) and D7 of adipocyte differentiation. nAnT-iCAGE sequencing was performed to analyse transcriptional start sites. Overall design: CAGE-seq analysis of D0 and D7 3T3-L1 cells before and after adipogenic differentiation was performed on 4 replicates each