RNA-seq experiment to investigate the impact of sulforaphane metabolism in HepG2 cells. Cells with NRF2 knockdown treated with sulforaphane were compared to untreated cells with NRF2 knock-down. Similarly, wild type cells treated with sulforaphane were compared to untreated cells.
This study aims to assess whether genes involved in central metabolism induced by Sulforaphane, SF, are mediated through the transcription factor NRF2. A time course RNA-seq experiment was performed, where RNA is extracted after 3, 9, and 24 hours SF treatment. This experiment tests the following hypotheses:  1) The transcriptional changes induced by SF at early time points will affect genes involved in the antioxidant response machinery of the cell.  2) The transcriptional changes induced by SF at 24 h will affect genes involved in central metabolism.  This is a follow up from a previous study of the effect of SF on HepG2 cells under different glucose environment (see E-MTAB-12851).