Uncovering the potential molecular mechanism of liraglutide to alleviate the effects of high glucose on myoblasts based on high-throughput transcriptome sequencing technique
Myoblasts play an important role in muscle growth and repair, but the high glucose environment severely affects the function of myoblasts. The purpose of this study is to explore the potential molecular mechanism of liraglutide in alleviating the effects of high glucose environments on myoblasts.High-throughput transcriptome sequencing technique was used to sequence C2C12 myoblast from different treated groups. Overall design: The sequencing samples in this study included C2C12 cell control group (NC group), C2C12 cell high glucose (25 mmol/L) treated group (H group) and C2C12 cell high glucose (25 mmol/L) + liraglutide (200 nmol/L) treated group (H+L group). RNA sequencing was performed to obtain the differentially expressed mRNAs (DEmRNAs).