Infiltration of inflammatory macrophages and neutrophils and widespread pyroptosis in lung drive influenza lethality in nonhuman primates
We established a model by which precise doses of H5N1 influenza could be delivered via aerosol into the lower lungs of macaques. We then allowed the animals to progress to an endpoint, collected organs, sorted cells, and performed RNA seq specifically on whole lung, interstitial macrophages, alveolar macrophages or neutrophils. Overall design: Compared mock infected animals to H5N1 infected animals