Regulons of VPA1682 and VP2427 in Vibrio parahaemolyticus RIMD2210633
Vibrio parahaemolyticus strains were grown in LB broth at 37 centigrade with shaking at 200 rpm. Bacetial cells were harvested at the mid-log phase gowth (OD600 value of about 1.5). Total RNAs was extracted. mRNA enrichment was performed using an Illumina/Ribo-ZeroTM rRNA Removal Kit (bacteria). Construction and sequencing of cDNA library was performed on an Illumina Hiseq platform.