IL-22 resolves MASLD via enterocyte STAT3 restoration of diet perturbed intestinal homeostasis [16s rDNA-seq]
IL22 induces antimicrobial peptides which influnce microbiota. We used 16s rRNA gene sequencing (16s DNA-seq) to analyze the microbiota with Fc or IL-22Fc treatment. Overall design: Male Il22ra1f/f, Il22ra1?Hep, Il22ra1?IEC and C57BL/6 mice with Abx (10 weeks old) were fed with HFD+30% fructose water for 16 weeks, and were intraperitoneally (i.p.) injected with 10µg mFc or rmIL-22-Fc /mouse every 3 days for last 4 weeks. We collected the colon feces from above mice were subjected to 16s DNA sequencing.