Host SYISL regulates offspring embryonic muscle formation by affecting maternal gut microbiota composition and butyrate levels
we collected female mouse fecal samples at 13.5, 15.5, and 18.5 d of pregnancy from WT and KO mice, and the composition of the fecal microbiota was analyzed by sequencing the V3~V4 hypervariable region of the 16S rRNA gene.