Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [ampliseq 3]
Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Map the off-target landscape of 1 sgRNAs targeting VEGFAsite2 with Streptococcus pyogenes (Sp)Cas9 using TTISS-seq