scRNA-seq of Mycobacterium tuberculosis-specific CD4 T cells from Kenyan women with and wihtout HIV and before and after ART initiation following stimulation with Mtb WCL
To understand how HIV and timing of ART initiation affect the transcriptional profile of Mtb-specific CD4 T cells, we performed 10X single-cell RNA sequencing comparing Mtb-specific CD4 T cell transcriptomes from people without HIV, people with HIV prior to ART, and people with HIV after ART from the Mombasa Cohort in Mombasa, Kenya. PBMCs were stimulated overnight with Mycobacterium tuberculosis whole cell lysate and CD40L+CD69+ Mtb-specifc CD4 T cells were sorted for sequencing. Overall design: scRNA-seq of Mtb-specific CD4 T cells after overnight stimulation with Mycobacterium tuberculosis whole cells lysate