Heterogeneity and Homogeneity in Treg responses to Interleukin-2  [scATAC-seq1: IL2 2h and PBS Ctl]
We investigated early effects of IL2 treatment on mouse Treg chromatin accessibility by performing scATAC-seq on splenic Tregs isolated from mice 2h after treatment with recombinant IL2 or PBS vehicle control. Overall design: Mouse CD4+ regulatory Tregs were isolated from Foxp3-IRES-GFP reporter mice exactly 2h after treatment with 10 µg of recombinant mouse IL2 (PeproTech, 212-12) or PBS vehicle control (in 110 µl volume). Tregs were isolated from the spleen by FACS for scATAC-seq.