iCLIP of Tra2b, Son-GFP and Srrm2-GFP in mESCs
In order to investigate the role of SR protein assemblies on GA-multivalent mRNA regions and their relationship to the nuclear speckle. We performed iCLIP of Tra2b, Son-GFP and Srrm2-GFP in mESCs. The GFP-tagged proteins are endogenously tagged and immunoprecipitated using an anti-GFP antibody, while Tra2b was immunoprecipitated using an antibody against the endogenous protein. Srrm2 and Son are very large proteins, but signal was observed at a variety of molecular weights along the membrane, suggesting indirect interactions. We isolated two fractions for each Srrm2 or Son sample: one 'high MW', cut at ~300kDa upwards, and one 'low MW' cut from 40 kDa to 300 kDa. These samples were prepared and sequenced separately.  The data provided here has been demultiplexed and trimmed, and therefore should not contain adapter sequences or barcodes. UMI sequences have been moved to the fastq header. Also included are transcriptomic coordinates of deduplicated crosslinks for each sample, using one transcript per gene, as used to plot transcriptomic metaprofiles around multivalent sites.