Single Cell RNA sequencing of the Monocytes from controls and pulmonary arterial hypertension (PAH) patients
We report the application of single cell RNA sequencing on human Peripheral Blood Mononuclear cells. The 12 samples are sequenced as biological replications (6 control and 6 patients), and pooled at the analysis stage. The library was prepared using the Chromium Single Cell 3' v2 and v3 Reagent Kit, and sample processing was under bcl2fastq2/2.20.0 and cellranger/2.2.0. We report a decreased CD14 in classical monocytes among PAH patients, with greater potential of apoptosis for CD14+ monocytes population. This study provide a reference for studying the monocytes related abonormalties in PAH and other inflammatory diseases. Overall design: Six control samples and six patient samples are barcoded for sequencing in the same flowcell on Illumina HiSeq-4000 platform. For each sample, 5000 alive cells are used as input.