RNA-sequencing of CD8+T and CD4+T cells from E4bp4-/- and WT mice induced by cGVHD
Using the method of bm12-cGVHD, lupus-like cGVHD models were established in E4bp4-/- mice and C57 mice. In addition, duplicate E4bp4-/- cGVHD groups were set up, but the entire process was carried out using CD8 intracellular blocking antibodies or PBS.The model establishment was completed at week 10. The results showed that the E4BP4 deficiency could accelerate the onset of cGVHD by promoting the expansion of CD8+ and CD4+T cells. Furthermore, the depletion of CD8+T cells effectively reversed the lupus-like phenotype, and the expansion of CD4+T cells in CD8+T-depleted mice were reversed either. Then sorting the CD8+T and CD4+T cells from the splenic lymphocytes of the recipient mice at the endpoint, high-throughput transcriptome sequencing was performed to explore the molecular mechanism of E4bp4 regulating the development of CD8+ T cells and to search for therapeutic targets for lupus.