A comparative Genomic Architecture and Functional Potential of Prophages in Clinical Granulicatella adiacens: From Prophage Cargo to Impact
Background: Granulicatella adiacens, an oral commensal and an opportunistic pathogen, with mobilome of limited genomic insights. Objectives: to characterize mobilome of the study isolate in relation to genomic architecture, evolutionary signals and potential clinical cargo. Methods: In silico analysis of prophages for further prediction and detection of gene content, prophage lifestyle, integration-associated characteristics by Python script with Biopython. Results and Discussion: mosaic assembly driven by horizontal gene transfer was evidential by GC content and deviation. Mobility and inedibility was indicated associated with poor inducibility but without ruling out possible induction especially under stress conditions of antimicrobial use and limited nutrition with the competition of polymicrobial communities in niches. Further evidence of niche adaptation as commensal of oral cavity promoted by phage-inducible islands. Conclusion: Surveillance of prophages as markers could aid in profiling and screening modules of integrases and resolve strain relatedness and provide understanding of unjustified phenotypic differences between isolates.