single cell RNA-seq of samples from Cdh5-creERt2/Shb flox/flox mice
E0771.lmb tumors were grown after tamoxifen injection as previously described in wild-type and Cdh5-creERt2/Shb flox/flox mice. Mice were sacrificed on day 14-16 after tumor cell injection. The tumors were digested, CD45+-cells isolated by MACS microbeads (Miltenyi, Bergich Gladbach, Germany) Overall design: single cell RNAseq was performed on 6 wild type and 6 KO samples in three separate experiments.