Bulk microglia RNA-sequencing after salt diet administration and repetitive close head injury
The associations between human concussions and subsequent sequelae of chronic neuropsychiatric and cardiovascular such as hypertension have been reported; however, little is known about the underlying biological processes. We hypothesized that dietary changes, including a high salt diet (HSD), disrupt the bidirectional gut-brain axis, resulting in worsening neuroinflammation and emergence of cardiovascular and behavioral phenotypes in the chronic period after repetitive closed head injury (rCHI) in adolescent mice. Adolescent mice were subjected to three daily closed head injuries, recovered for 12 weeks, and then maintained on HSD or normal diet (ND) for an additional 12 weeks. Here, we have obtained bulk microglia RNA-sequencing of four sample groups: Sham ND, Sham HSD, rCHI ND, and rCHI HSD.