Targeted DNA and surface protein sequencing at single cell level of BM MNCs and PB MNCs from CCUS patients
We performed high-throughput single-cell targeted DNA and surface protein sequencing (scDNA + protein-seq) using the Mission Bio Tapestri Platform to analyze NK cells isolated from five patients with CCUS exhibiting different genetic alterations. Our analysis revealed that the NK cells were part of the mutant clone population, displaying a clonal burden comparable to that observed in the myeloid cells of the same patients. This dataset provides valuable insights into the clonal architecture of NK cells in the context of CCUS. Overall design: BM and PB MNCs were isolated by Fluorescence-activated cell sorting and analyzed using scDNA + protein-seq