Epimedium brevicornu Raw sequence reads
E. brevicornu is considered a traditional Chinese medicine with important commercial value, however, its seed dormancy release has been a limiting factor for large-scale artificial cultivation. In this study, we optimized the method of seed dormancy release in E. brevicornu, compared the performance of seeds with different stratification treatments in terms of embryo percentage and germination percentage, and explored the gene expression patterns under different stratification treatments using RNA-seq technology. We expect to reveal the relationship between stratification treatment and seed dormancy, and to explore the key genes regulating seed dormancy in E. brevicornu, so as to provide effective candidate genes and a theoretical basis for improving and creating non-dormant superior E. brevicornu germplasm.