RNA-seq analysis of SMYD2-silenced prostate cancer cells C4-2 and ChIP-seq analysis for both SMYD2-silenced and SMYD2-overexpressed C4-2 cells
Total RNA was obtained from C4-2 cells with or without SMYD2 silence to perform a high-throughput sequencing to explore the expression profile changes caused by SMYD2 in prostate cancer. AR chromatin immunoprecipitation was conducted using SMYD2-silenced, SMYD2-overexpressed, and parent C4-2 cells. Then, the chromatin immunoprecipitates were extracted to perform high-throughput sequencing to investigate the influence of SMYD2 on AR genomic binding profiles.