Effect of TGFb on HASMC phenotype after 7 days of treatment
We find that TGFß signaling in SMCs induced ACSS2-dependent production of Ac-CoA in a PDK4-dependent manner. TGFb induces SMC contractile phenotype and glycolysis. Overall design: Human aortic primary smooth muscle cells (HASMCs) were treated with 10 ng/mL of TGFb for 7 daysm with medium changes every other day.