Glucocorticoid Receptor cistromic reprogramming by caloric restriction and corticosterone treatments.
In order to examine the impact of two separate corticosterone surges on liver physiology, we conducted genome-wide mapping of GR binding profiles at 6 pm (ZT12) through ChIP-sequencing. This was done using liver samples from mice subjected to either short-term caloric restriction or corticosterone injections. Our findings reveal that both interventions yield unique binding profiles. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Glucocorticoid Receptor (GR) in mouse liver in caloric restriction. In the case of GR, we have ChIP-seq between three different conditions (night-restricted feeding/NR, caloric restriction/CR, and cort-supplementation + NR/CORT+NR).