Single-cell copy number analysis in melanoma
We performed shallow single-cell copy number analysis across 1,475 cells from a melanoma COLO829 line, to resolve overall tumor complexity and clonality. We identified at least four major sub-clones by discriminant analysis of principal components (DAPC). Re-analysis of previous spectral karyotyping data recapitulated these sub-clone hallmark features and explains why previous experiments generated conflicting results. Overall, our results demonstrate how single cell sequencing can uncover significant hidden insights in a melanoma sample. Overall design: Copy number of analysis of 1,475 cells from COLO829 melanoma