Synthetic community sequencing for SaxA public good study
We investigated the role of the isothiocyanate (ITC)-degrading bacterial enzyme SaxA in a microbial community context. We used five ITC-sensitive bacterial commensals combined with either the opportunistic pathogen Pseudomonas viridiflava 3D9 (PS) or a 3D9 dSaxA knockout mutant with and without ITC. The in-vitro culture was grown and samples taken over time, using an internal microbial standard to quantify microbial growth. This setup allows us to investigate the role of SaxA on ITC-sensitive bacterial growth in a microbial community context.