Pleiotropic CAR-M for the Treatment of Myocardial Infarction
Description: Myocardial infarction (MI) triggers extensive cardiac remodeling characterized by inflammatory and fibrotic responses. To explore immune-modulatory strategies that can influence post-infarction remodeling, we employed pleiotropic chimeric antigen receptor–modified cells (CAR-M) designed for targeted immune intervention within the injured heart. Single-cell RNA sequencing (scRNA-seq) was performed to characterize the cellular composition and transcriptional changes in cardiac tissues from mice subjected to experimental MI, either untreated or treated with CAR-M. This dataset enables the investigation of how pleiotropic CAR-M influence immune and stromal cell populations in the infarcted microenvironment. Overall design: Myocardial infarction was induced in 6-8-week-old C57BL/6J mice by permanent ligation of the left anterior descending (LAD) coronary artery. Mice were divided into two groups: (1) MI (vehicle-treated) and (2) MI + CAR-M (treated with CAR-M via systemic infusion after MI induction). Cardiac tissues (left ventricle) were collected at 7 days post-treatment, enzymatically dissociated into single-cell suspensions, and subjected to single-cell RNA sequencing using the 10x Genomics Chromium platform. The dataset provides a resource for studying cellular heterogeneity and immune modulation associated with pleiotropic CAR-M treatment following MI.