Functional and Molecular Single-Cell Analyses Implicate PRDM14 in the Initiation of a Novel B-1 Cell Malignancy - Cut and Run experiments
Molecular mechanisms underlying high-risk subtypes of leukemia remain elusive. PR domain-containing 14 (Prdm14) is a potent oncogene implicated in the initiation of many cancers, including leukemia. Here, we interrogate the heterogeneity of Prdm14-expressing cell types in a mouse model of T-cell acute lymphoblastic leukemia (T-ALL). We identified an abnormal B-1 cell-like population in pre-leukemic bone marrow. B-1 cells are a self-renewing population of unconventional B cells established during embryonic development. This dataset contains genome-wide profiling of PRDM14, H3K4me1, and H3K4me3 to understand the genome-wide binding of the key TF in the abnormal B1 population and their B-cell conserved and B-1 cell-type specific enhancer and promoter regions. Cut-and-run experiments for each antibody were completed in triplicate, with IgG included as an extra experimental control.