DNA sequencing of plasmid pools for CRISPR-Cas/CAST PAM analysis
To compare PAM requirements between canonical I-F P. aeruginosa PA14 CRISPR-Cas system and I-F3b Tn6900 CRISPR-asssociated transposon (CAST), plasmid pool with degenerate sequence at a PAM site was sequenced before and after selecting for either CRISPR-Cas interference or CAST transposition.