Effect of norepinephrine and UK 14,304 treatment on murine small intestinal organoids
Murine small intestinal organoids were stimulated with the general a and ß adrenergic receptor agonist norepinephrine and the complete potent and specific a2 adrenergic agonist UK 14,304 (both 1uM) or vehicle controls for 4 days. Afterwards, they were harvested for RNA isolation and proliferation and differentiation gene expression patterns were assessed. Overall design: Organoids treated with vehicle control or agonist: vehicle control HCl for norepinephrine (n=4), vehicle control DMSO for UK 14,304 (n=4), norepinephrine 1uM (n=5), UK 14,304 1uM (n=5)