Brassica napus cultivar:Westar Raw sequence reads
Canola (Brassica napus L.) is a commercially important oilseed crop worldwide. However, several devastating diseases such as clubroot, sclerotinia stem rot, and blackleg threatens the canola industry. The use of resistant germplasm is the best strategy to stop pathogen spreading and the economic loses linked to those diseases. To date, most resistance genes encode intracellular nucleotide binding leucine-rich repeat (NLR) immune receptor proteins (Barragan et al., 2021). Genome-wide identification of the NLR gene family in B. napus can help breeders and researchers accelerate the search for resistance genes. The B. napus cultivar "Westar" is a spring-type, low erucic acid cultivar that is widely used in genetic mapping and transformation studies. It also serves as a universal susceptible host standard for studying canola disease resistance to pathogens. The genome of "Westar" was released in 2020 and is predicted to carry 97,514 annotated genes (Song et al., 2020). Scanning those 97,514 genes for the presence of NLR-associated domains revealed a significantly lower number of NLRs compared to other annotated B. napus genomes (Alamery et al., 2018; Chen et al., 2021). This made us wonder if the "Westar" canola genome was properly annotated. In this study, we produced the first canola NLRome using the "Westar" genome and resistance gene enrichment sequencing (RenSeq) approach. This SRA repository contains the Illumina raw reads for the RenSeq experiment. The availability of the high-quality "Westar" NLRome is not only a significant advancement in understanding canola genetics but also offers practical applications for breeding programs aimed at improving disease resistance.