Transcriptome sequencing of liver injury models
The liver is the largest solid organ and digestive gland in the human body. It plays a key role in the metabolism, transformation and detoxification of the three major organic substances. Therefore, the liver occupies an important place in the body's metabolism of substances. Crizotinib is a new type of drug with high efficiency for the treatment of lung cancer, but it has a large toxic effect, so this paper studies the acute effect of crizotinib on the liver, and studies its key targets for toxic effects through high-throughput transcriptome sequencing, which is divided into normal group and administration group, each group n=3, and the samples are mouse tissue samples. Overall design: CITE-seg: ICR mice were randomly divided into dosing and normal groups. After 7 days of adaptation, the normal group was given 300mg/kg of crizotinib based on the same dose of normal saline, and three mice from each group were randomly selected for high-throughput transcriptome sequencing based on the same dose of normal saline, and samples were collected one week after administration.