genome sequencing of T. reesei transformed strains with an egp under the control of tef1 promoter
Independent transformants obtained by a random integration into the genome of a green fluorescence expression cassette with the constitutive tef1 promoter and the cbh1 terminator circular plasmid containing the expression cassette showed a wide range of fluorescence levels. Whole genome sequencing was conducted on eight of the transformed strains using two NGS platforms : Illumina paired-end sequencing and Oxford Nanopore to identify the integration sites of the plasmid