Biological stress profiling of Japanese medaka (Oryzias latipes) using eRNA
We investigated the potential of environmental RNA (eRNA) to assess the health status of Japanese medaka fish (Oryzias latipes) exposed to linear alkenyl sulfate (LAS). Analyzing eRNA and organismal RNA (oRNA) in aquarium water for a short period (~12 h) after the onset of exposure. Conclusively, eRNA-seq using aquarium water emerges as a valuable non-invasive tool for analyzing physiological stress. The findings of this study lay the foundation for the further development of eRNA-seq technologies.