Gut Microbiota Alterations Mediated by L. plantarum HYY-DB36 in DSS-Colitis Model
This project provides gut microbiota sequencing data from mouse fecal samples, focusing on the analysis of bacterial community composition. Total genomic DNA was extracted from mouse fecal samples using the Omega Soil DNA Kit. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified by PCR with universal primers 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3').The obtained amplicons were sequenced, and the sequencing data were clustered into operational taxonomic units (OTUs). Representative sequences of each OTU were taxonomically annotated using the Ribosomal Database Project and SILVA database to determine the microbial community composition. The uploaded data include raw sequencing reads, OTU clustering results, and taxonomic annotation information. These data are publicly shared to support further research on gut microbial diversity and community structure, and to facilitate relevant comparative and verification studies.