Transcriptome Sequencing of Nurr1 knockdown cells and the controls
Parkinsons disease (PD) is a progressive neurodegenerative disease characterized by the degeneration of dopaminergic neurons in the substantia nigra and the accumulation of misfolded proteins within susceptible neurons. Nuclear receptor related protein 1 (Nurr1), an orphan nuclear receptor, is widely involved in various signaling pathways associated with human diseases, particularly neurological and neurodegenerative diseases. As a transcription factor, Nurr1 regulates the expression of tyrosine hydroxylase (TH), aromatic amino acid decarboxylase (AADC) and the dopamine transporter (DAT) which are essential for the synthesis, storage, and release of dopamine, indicating that Nurr1 has a vital role in maintaining the differentiation and maturation of dopaminergic neurons. We genarated stable Nurr1 knockdown mouse neuroblastoma N2a cells and relevant control cells. Total RNA of Nurr1 knockdown and control cells was extracted and evaluated. The libraries were constructed by Truseq Stranded mRNA LTSample Prep Kit according to the manufacturers instruction and then sequenced on the Illumina sequencing platform. Advanced analysis of transcriptome sequencing with reference genes was conducted to see the alterations in the transcriptome of Nurr1 knockdown N2a cells. The results will throw light upon new pathogenesis of Parkinsons disease.