Small RNA Library Sequencing of IL4/IL13 Treated Prostate Fibroblasts
Interleukin IL-4 and IL-13 JAK/STAT signaling increases fibrosis-related extracellular membrane (ECM) protein production in prostate fibroblasts, which contributes to aging-associated lower urinary tract dysfunction (LUTD). IL-4 and IL-13 also differentially regulate several species of regulatory RNAs, including microRNA (miRNA) and circular RNA (circRNA). In the studies described here, complementary nucleotide binding interactions between these RNA species were used to develop a competitive endogenous RNA (ceRNA) network to elucidate interactions that could potentially increase ECM protein production during fibrotic development. To accomplish this, small RNA was extracted from prostate stromal fibroblasts induced by IL-4 or IL-13 and subjected to sequencing analysis to identify differentially expressed miRNAs and circRNAs