Phenotypic heterogeneity follows an evolutionary growth-lifespan tradeoff in suboptimal conditions
S. cerevisiae cells growing exponentially in rich media (YPD) were washed twice in PBS and resuspended in nitrogen limitation media containing 0.8 mM Proline as nitrogen source. This shift induced cellular differentiation into two subpopulations of difference cell size and phenotypes. Here, we study these differences using transcriptomics (RNAseq) of each subpopulation (5 biological replicates for each subpopulation), 1 hour into the shift.