RNA-seq of CYR61-treated THP1 human monocytes
We assess the effects of CYR61 treatment on the transcriptome of human THP1 monocytes. Overall design: We used THP1 monocytes (ATCC TIB-202). These cells were maintained in RPMI 1640 media containing 1% L-glutamine, 1% penicillin/streptomycin, and 10% FBS. We treated THP1 cells with recombinant human CYR61 (PeproTech 120-25): 0.00, 0.05, 0.50, and 5.00 µg/ml for 24 hr. After incubation, we washed cells with PBS, isolated total RNA, and prepared samples for RNA-sequencing.