16S rRNA sequencing of fecal microbiota in APC mice treated with aspartame
Fecal microbiota from APC mice treated with aspartame or vehicle control (4 per group) were analyzed by 16S rRNA gene amplicon sequencing. Bacterial genomic DNA was extracted from fecal samples, and the V3 -V4 hypervariable regions of the 16S rRNA gene were amplified by PCR. Sequencing libraries were prepared according to standard protocols and sequenced on the Illumina NextSeq 2000 platform. Raw sequencing reads were processed for quality control, taxonomic assignment, and microbial community profiling. This dataset enables comparative analysis of gut microbiota composition and diversity associated with aspartame exposure in a mouse model of spontaneous intestinal tumorigenesis.