Macrophage recipient WGBS data: Donors infected with Porphyromonas gingivali (WDPg) or sham-infected (WD) and fed a western diet (WD)
Our understanding of how atherosclerosisr risk factors persist after risk factor mitigation is incomplete. Here, we use oral infection with Porphyromonas gingivalis (Pg) as a model risk factor. Atherosclerosis-prone LDLR Komice were infected with Pg or sham infected, and fed a high fat diet for 16 weeks. Their bone marrow was then injected into lethally irradiated naive LDLR KO mice. After 16 weeks of high fat diet feeding, macrophages were elicited into the peritoneal cavity with thioglycollate. DNA from these macrophages was isolated for WGBS sequencing. We hypothesize that oral infection with Pg caused methylation differences in genes that contribute to increased atherosclerosis. Overall design: DNA from elicited macrophages. 5 mice were pooled/group. WD=sham infected mice. WD+Pg=mice orally infected with Pg.