Timecourse analysis of human colorectal cancer organoids under FOLFIRI chemotherapy
Two human colorectal cancer organoids derived from liver metastasis of the same patient were grown for seven days, then treated with FOLFIRI chemotherapy in vitro and sampled at different timepoints for single-cell RNA-sequencing. Time points include 0 h, 12 h, 24 h, 48 h, 72 h and 120 h on-treatment as well as 12 h, 24 h, 48 h and 72 h washout after a 72 h treatment period. Samples were multiplexed using hashtag oligos prior to sequencing.