Whole Genome Sequencing and Sequence Analysis of lipase-producing Staphylococcus xylosus YCC3
For a better understanding of its metabolic mechanism and function during sausages fermentation, the whole genome of Staphylococcus xylosus YCC3, a strain with high lipase activity isolated from fermented meat products was sequenced using the PromethION and Illumina HiSeq sequencing platform. The results showed that the genome of YCC3 was a set of circular molecules containing 3 plasmids, and the total length of the genome sequence was 2,773,035 bp with a GC content of 32.88 %. It was predicted to contain 2540 protein-coding genes with a total length of 2,742,136 bp, an average length of 1079.58 bp, accounting for 83.24 % of the genome. The genome of YCC3 contained 22 rRNA operons and 59 tRNA, 16 fatty acid synthesis, 10 fatty acid degradation and 2 unsaturated fatty acid synthesis.genes being predicted from the genome. Moreover, functional annotation of the genome and metabolic pathway information annotation were accomplished by aligning the coding genes to the GO, COG, KEGG and PHI databases, which would provide a theoretical basis to explore the application of strain YCC3.