Genome resequencing of adaptive laboratory evolved Bacillus subtilis ATCC 6633 strain for heat-shock resistance
Each sample was prepared from adaptive laboratory evolution of ATCC 6633 strain for heat-shock resistance. Evolution was conducted with iterative cycles of sporulation in DSM medium and heat-shock using boiling water. Genomic DNAs were prepared by using Mag-Bind Bacterial DNA purification kit from Omega Bio-Tek (Norcross, GA, USA) and converted into a sequencing library by using NEBNext Ultra II DNA Library Prep Kit (Ipswich, MA, USA). Sequencing was performed using Illumina Novaseq 6000 (San Diego, CA, USA) at UC San Diego IGM Genomics Center.