2 -step metabarcoding of soil samples methodology
We developed a novel approach to obtain more accurate and detailed data on soil microbiome structure and biodiversity. The first step involves sequencing of amplicons generated using universal 16S primers. This method provides an initial overview of the microbial community, and allows the identification of key taxonomical groups. In the second step, we employ sequencing of amplicons generated with taxa- specific primers designed for the most abundant phyla in the community.