Effect of resistant-derived extracellular vesicles on breast cancer cells acquired drug resistance process
The acquired resistance that tumors develop during the treatment is still a central cause of cancer-associated deaths. Our study investigates the transcription changes modulated by EVs from tamoxifen- and doxorubicin-resistant breast cancer cells in sensitive cells and how these changes may induce acquired drug resistance, inhibit apoptosis, and increase survivability in the sensitive cells. Overall design: To better understand the influence of drug-resistant EVs on the sensitive MCF-7 and MDA-MB-231 transcriptomes, we performed RNA sequencing (RNA-Seq) analysis in these two cell lines after exposure to tamoxifen-resistant and doxorubicin-resistant EVs for 24 h. The transcriptomic profilling was performed using 3 biological replicates of RNA from sensitive MCF-7 and MDA-MB-231 cells in comparisson with RNA from tamoxifen and doxorubicin-resistant counterparts.