The Effects of MIZ1 Deficiency on Germinal Center B Cells in the Light Zone [RNA-seq]
The germinal center (GC) reaction is a critical process for the generation of high-affinity antibodies and the establishment of immunological memory. Within the GC, B cells undergo a series of tightly regulated molecular and cellular events. MIZ1, a transcription factor, has recently emerged as a key regulator of B cell fate during the GC response. This study aims to investigate the impact of MIZ1 deficiency specifically on B cells located in the light zone (LZ) of the germinal center. Overall design: Control and MIZ1KO mice were intravenously immunized with sheep red blood cells (SRBCs). Ten days post-immunization, spleens were collected from both groups. Light zone (LZ) germinal center (GC) B cells were sorted from the spleens of both control and MIZ1KO mice and subsequently subjected to bulk RNA sequencing (RNA-seq).