Bulk RNA sequencing of the differentiated bone marrow cells of Gpr137b-KO and -WT mice.
This study was performed to elucidate the possible functions of Gpr137b, an orphan GPCR, in the differentiation of bone marrow cells (BMCs) into macrophages/monocytes and osteoclasts. BMCs isolated from the C57BL/6 mice in which the Gpr137b gene was knocked out by Crispr/Cas9-based genome editing were subjected to hemolysis to remove erythrocytes and cultured in the presence of M-CSF only or M-CSF plus RANKL to induce their differentiation into macrophages and osteoclasts, respectively.