Amplicon sequencing data of microorganisms present in the rumen and hindgut of Charolais cattle fed rapeseed oil or unsupplemented cattle
The aim of the study was to carryout a characterisation of the rumen/faecal microbiota of cattle fed rapeseed oil compared to unsupplemented cattle. This was to identify potential modes of action of rapeseed oil that led to reduced enteric methane emissions in the rumen and the overall impact of rapeseed oil on gut microbial health. The groups of interest included bacteria, archaea, fungi and ciliated protozoa. For identification of bacterial populations the 16S rRNA gene was targeted. For Archaea, the V3 to V4 hypervariable regions of the 16S rRNA gene were targeted. The 18S rRNA gene region of protozoa was targeted, and for fungi the D1to D2 regions of the 28S rRNA gene.