Differential expression of genes affected by CRY1 or CRY2 in the absence of PER and NR1D proteins
The transcription-translation feedback loop, the core clock mechanism, is required for circadian rhythm. CRY protein, including CRY1 and CRY2, plays an important repressor role in the regulation of clock genes. However, other proteins, like PER1, PER2, NR1D1 and NR1D2, in the loop mask the transcriptional effects of CRY. This study provides data to find candidate genes specifically affected by CRY1 or CRY2 in mouse embryonic fibroblast (MEF) cells. Overall design: This study contains three types of samples: 1. MEF cells lacking CRY1, CRY2, PER1, PER2, NR1D1 and NR1D2 proteins (CPN-/-), 2. Exogenous CRY1 expression in the CPN-/- cells , 3.Exogenous CRY2 expression in the CPN-/- cells