RNA-Seq and Ribo-Seq analyses of elo3-6 (lacking functional Elongator complex) and urm11urm12 seedlings  (lacking functional URM1 like proteins 11 and 12)
The goal of this project is to study RNA expression (RNA-Seq) and translation efficiency (Ribo-seq) in 4-day-old Elongator mutant (elo3-6), URM1-like proteins 11 and 12 double mutant (urm11urm12), and wild-type (Col-0) Arabidopsis seedlings during photomorphogenesis. elo3-6 plants carry a mutation in the ELP3 gene encoding the catalytic subunit of the Elongator complex. This complex regulates gene expression during transcription and translation via epigenetic and tRNA wobble uridine modifications, respectively. Seedlings of elo3-6 show photomorphogenesis defect known as light hyposensitivity (elongated hypocotyls, reduced cotyledon opening angle and hyponastic growth of the cotyledons). URM1-like proteins 11 and 12 are involved in the thiolation of tRNA wobble uridine. Modifications around the anticodon loop affect decoding accuracy and codon-anticodon pairing fidelity, thereby fine-tuning protein translation.