Single-cell RNA sequencing of DJ-1 inhibition in tumor microenvironment
This dataset contains raw single-cell RNA sequencing data generated from tumor-infiltrating CD45? immune cells in murine cancer models to investigate the role of DJ-1 (PARK7) in modulating anti-tumor immunity. In the associated study, genetic ablation of DJ-1 enhanced the efficacy of immune checkpoint blockade by indirectly activating T cells through macrophage reprogramming. Loss of DJ-1 increased reactive oxygen species (ROS) levels in macrophages and promoting differentiation into Cxcl9? immune-stimulatory phenotypes while reducing Spp1? immune-suppressive subsets.  Here, we deposited additional dataset includes raw sequencing reads in FASTQ format from sorted CD45? cells (DJ-1 knockout and wild-type conditions).  A detailed description of experimental procedures and analysis workflows can be found in the associated publication deposited alongside this dataset.