Genome-wide mapping of DsbR-binding sites
We firstly found that DsbRS TCS was involved in counteracting the toxic effects of copper.The identification of two DsbR binding reigions in dsbD-dsbR intergenic region DNA led us to globally characterize all DsbR-binding loci on the chromosome of Pseudomonas aeruginosa.In order to express C-terminal flag-tagged DsbR, we first constructed the indicated strain.We next investigated DsbR-binding to the chromosome of this strain during exponential growth by ChIP-Seq, chromatin immunoprecipitation followed by ultra-high throughput DNA sequencing. Sequence reads obtained from three independent ChIP-Seq experiments using Flag-specific antibodies were mapped to the genome of the the indicated strain strain. Using MACS software, we identified 42 enriched loci (P-value < e-5) harboring DsbR-binding peaks. Collectively, this study thus uncover a new signal transduction pathway in P. aeruginosa, DsbS/DsbR/DsbDEG and DsbB, for the regulation of Dsb system in response to copper stress.