soil metagenome
This project aims to investigate the effects of arbuscular mycorrhizal fungi (AMF, specifically Funneliformis mosseae) on the rhizosphere microbial communities of tobacco (Nicotiana tabacum L.) under continuous cropping conditions. A controlled pot experiment was conducted using red soil collected from agricultural fields in Luoping County, Yunnan Province, China. The experiment included two soil types: 5-year continuous tobacco cropping soil and non-continuous cropping soil, both with and without AMF inoculation. High-throughput sequencing of the bacterial 16S rRNA gene (V3-V4 region) and the fungal ITS rRNA gene (ITS2 region) was performed on the rhizosphere soil samples to understand how AMF alleviates continuous cropping obstacles by reshaping the soil microbiome.