Transcriptome and HIF1a/CBP genomic distribution change in LUCAT1 knockdown in GSCs under hypoxia
Hypoxia is associated with poor prognosis in many cancers including glioblastoma (GBM). Glioma stem-like cells (GSCs) often reside in hypoxic regions and serve as reservoirs for disease progression. Long non-coding RNAs (lncRNAs) have been implicated in GBM. However, the lncRNAs that modulate GSC adaptations to hypoxia are poorly understood. Here, we identified a lncRNA LUCAT1 induced by hypoxia in GSCs. We performed RNA-seq to examine the gene expression change in GSCs under hypoxia after LUCAT1 knockdown. We demonstrated that LUCAT1 regulated HIF1a pathway and perform ChIP-seq to study CBP and HIF1a genomic binding sites in LUCAT1 knockdown GSCs under hypoxia.