Streptomyces sp. 11x1 Genome sequencing
Oomycetes are devastating soil-borne plant pathogens, and cause numerous losses on diverse crops worldwide. In this study, our aim was to identify Actinomycetes with antimicrobial activity towards oomycetes from the rhizosphere of soybean. Strong growth inhibition of P. myriotylum was found in dual culture with a strain called 11x1, and from using the 11x1 cell-free culture filtrate. The 11x1 strain was identified as a Streptomyces strain with the highest 16S rDNA sequence identity to S. scabiei. Applying the 11x1 culture filtrates to ginger and soybean leaves inhibited the necrosis caused by P. myriotylum and P. sojae, respectively. Pot assay experiments showed a strong control effect of the 11x1 culture filtrate against P. myriotylum-caused soft-rot disease of ginger, and against P. sojae caused root-rot of soybean. The 11x1 culture filtrate could also inhibit the growth of several other major Pythium and Phytophthora plant pathogens. Taken together, the culture filtrate of Streptomyces sp. strain 11x1 has potential for application to control ginger soft rot disease caused by P. myriotylum, as well as P. sojae caused root-rot of soybean. The genome of the 11x1 strain was used for the analysis of biosynthetic gene clusters (BGCs) and other functional categories predicted from the genome. The Streptomyces sp. strain 11x1 is an important new bioresource for the control of oomycete-caused diseases.