Tracing colonic embryonic transcriptional programs and their reactivation in inflammatory disease at single cell level
Purpose: determine transcriptional programs active during hindgut development and their reactivation in inflammatory disease; Summary: Our analyses reveal that most adult cell lineages are established by the late embryonic stage. In addition we find reactivation of embryonic features in inflammatory disease. Overall design: All embryonic (E14.5, E15.5, E18.5) and adult (DSS treatment) cells were sorted using FACS (using EpCAM and CD45 antibodies). Single cell RNA sequencing was perfomed (for details see Fazilaty et al, in prep)