Sulfur autotrophic denitrification 16S rRNA high throughput sequencing
To monitor the microbial community, the mixed total sludge was regularly collected at the end of each Phase of operation (i.e., seed sludge, and day 15, 40, 89, and 104 with different S/N ratios) for 16S rRNA amplicon sequencing with an Illumina MiSeq platform.