denitrification and elevated temperature
Our study reveals for the first time that the absolute copies of denitrifying genes, the denitrifying community abundance, and denitrifying community diversity decrease with increasing temperature in water contaminated by cadaver decay, which may bring some pressure on the environmental N cycle. Denitrification community structures were most affected by cadaver decomposition, but less affected by temperature. Tepidimonas, Bosea, Hydrogenophaga abundances and narG, nirS, norB, nosZ gene copies may predict changes in denitrification intensity by more than 70%. Temperature can indirectly change the denitrification intensity by regulating the abundance of denitrifying microbial communities. However, the laboratory environment cannot fully represent the changing field environment, so our control experiments had some limitations in the prediction of the elevated temperature by the denitrification process and denitrification intensity. Our subsequent studies will focus on the process of nitrogen and carbon cycling produced from animal cadavers under global warming by using metagenomics.