Genome sequencing of CRISPR-edited cells using lyophilized nanoparticle delivery
This study aimed to develop a stable and scalable delivery system for CRISPR-Cas9 ribonucleoprotein (RNP) complexes using freeze-dried inorganic nanoparticles. By embedding RNPs into nanoporous silica-based carriers and lyophilizing the formulation, the platform provides enhanced protection and delivery efficiency of gene-editing cargo. DNA sequencing was performed to validate genome editing outcomes in treated cells, confirming the efficacy of the nanoparticle-mediated delivery. This system presents a promising approach for gene therapy applications by improving the stability and usability of CRISPR-based therapeutics.