Low-abundance mRNA-sequencing of DN1 phase T cells from 6-month-old thymic Bmi-1 conditional knockout mice and control littermates
We used low-abundance mRNA amplification transcriptome sequencing to analyze the expression changes of different genes in DN1 T cells in the thymus tissue of 6-month-old mice with thymocyte-specific condition knockout of Bmi-1 (Lck cre+ Bmi-1f/f) and control (Bmi-1f/f) littermates. Due to the low proportion of DN1 phase cells in thymic T cells, each group of cells was taken from thymus of 5 mice. Thymic CD4-CD8-double negative cells were separated by magnetic beads, and then CD44+CD25- DN1 phase T cells were separated by flow cytometry. Differentially expressed genes (DEGs) were screened by "FindAllMarkers" analysis (| log2FC |>1, p value<0.05). Use the "ClusterProfiler" to perform GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis on differential genes, and visualize the KEGG and GO pathway enrichment analysis results using the "enrichplot" package.