Rapid systemic responses of Arabidopsis to waterlogging stress are mediated by the ROS wave
We show that an abrupt waterlogging treatment of Arabidopsis thaliana plants triggers a systemic ROS and calcium wave response, and that the waterlogging-triggered ROS wave response is dependent on RBOHD, calcium-permeable channels GLR3.3 and GLR3.6, and aquaporin PIP2;1 proteins. We further show that waterlogging stress is accompanied by a rapid systemic transcriptomic response that is evident as early as 10 min following waterlogging initiation and is partially dependent on RBOHD. Interestingly, the abrupt waterlogging stress resulted in the triggering of a rapid hydraulic wave response and the transient opening of stomata on leaves. Taken together, our findings reveal that the initiation of waterlogging stress in plants is accompanied by rapid systemic transcriptomic and physiological responses that involve the ROS, calcium, and hydraulic waves. These findings reveal that systemic plant responses to waterlogging stress are rapid and at least partially dependent on cell-to-cell signaling mechanisms. Overall design: 30 samples were analyzed; included two genotypes (Col-0 and rbohD); 4 time points for Col-0 (0,10,30,60 min) and 2 time points for rbohD (0 and 60 min); systemic leaves; 3 repeats composed from 15 plants for each genotype in each time point; Col-0 and 0min were control/reference samples