Single cell sequencing of CD45+ PBMCs isolated from mice w/ or w/o H.felis infection
Single cell suspension of PBMCs was isolated from mice w/ or w/o H.felis infection and then flow-sorted to collect CD45+ immune cells. There were 4 mice in each library. Each cell suspension was subjected to 3' single-cell sequencing using 10x Chromium Controller with accompanying Chromium 3' CellPlex Kit.Libraries were sequenced on Illumina NovaSeq 6000 platform.