Transcriptome analysis by total RNA sequencing of genetically modified Rhodobacter capsulatus strains for inducible expression of cas13a
In this study we analyzed the effects of an altered Cas13a expression in vivo, in presence or absence of ectopically expressed protospacer RNA (plasmid pCV2_SB6), by RNA-sequencing. The genomic modification of the cas13a controlling promoter enables altered expression of cas13a by adding either subinhibitory crystal violet (CV) concentrations (for transcriptional repression of cas13a), or additionally IPTG (for transcriptional induction of cas13a), to the mutant cultures. Transcriptome profiles were compared to corresponding wild type controls. Overall design: Comparative gene expression profiling analysis of RNA-seq data of independent biological triplicates of Rhodobacter capsulatus strains, grown either under cas13a inducing or cas13a repressing conditions.