cDNA sequencing from mRNA-display based selection of random sequence libraries, studies for in vitro selection of translational arrest sequence
Translational arrest is pausing or slowdown of translation elongation on ribosome caused by nascent peptide itself. The goal of this project is to establish novel method for large-scale in vitro selection of translational arrest peptides from DNA libraries by combining a modified mRNA display method and deep sequencing with eukaryotic and bacterial translation system.