Interaction of the RNA helicase Ighmbp2 with ribosomes
Ighmbp2 localizes to the cytoplasm where it associates with ribosomes and polysomes. Here we mapped the interaction site of Ighmbp2 on the ribosome. To detect potential RNA binding sites, recombinant GST-Ighmbp2-His6 was bound to isolated ribosomes in vitro and irradiated by UV-light. After partial RNase digestion, the cross-linked rRNA fragments were identified by Illumina sequencing