Bulk RNA-sequencing of human intestinal organoid cells
We focused on a rare cell population of human intestine, the M cells. Using human intestinal organoid as a model, the M cells could be differentiated, FACS-enriched and analyzed by RNA-seq. Overall design: Human intestinal organoids were cultured in M cell differentiation medium. Cell subpopulations derived from the differentiated M cell orgnaoids were FACS sorted and collected in TRIzol for bulk RNA-seq analysis.