16S seq SPRUCE Aug16 Raw sequence reads
The SPRUCE project involves an ecosystem-scale climate manipulation in S1 Bog. Intact soil core samples were collected from 11 depth intervals (0-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-125, 125-150, 150-175 and 175-200 cm) at each of the 10 SPRUCE experimental plots in June 2014 and June 2015. The diversity and composition of prokaryotic communities was determined by applying a high-throughput sequencing-based protocol that targets PCR-generated amplicons from V4 variable regions of the 16S rRNA gene using the bacterial primer set 515F and 806R