CLIP-seq analysis for glucocorticoid receptor-mediated mRNA decay
The goal is to identify endogenous GR-mediated mRNA decay (GMD) target transcripts and its biological relevance. Cross-linking and immunoprecipitation coupled with high-throughput sequencing (CLIP-seq) analysis for GMD factors (GR, YBX1, and HRSP12/UK114) were conducted for identification of endogenous GMD substrates.