GeneBits: ultra-sensitive tumour-informed ctDNA monitoring of treatment response and relapse in cancer patients
GeneBits describes a worfklow for rapid tumour-informed monitoring of somatic variants in cell-free DNA (cfDNA) from liquid biopsies. For benchmarking of GeneBits and the bioinformatics pipeline umiVar, different commercial cfDNA reference standards with known variant allele frequencies were tested. Small enrichment panels were designed for each reference standard, and employedfor the targeted NGS workflows from either IDT or Twist.