RNA sequencing of Polygonatum cyrtonema seeds at three different  germination stages
We used three different stages of the Polygonatum cyrtonema seeds (the fresh harvested seed, named S1, the emergence of the radicle, named S2 and the phase between primary rhizome and cotyledon emergence, named S3) to study the mechanism seed germination. Transcriptome analysis showed that there were 11,565 differentially expressed genes (DEGs) between S1 and S2, 10,196 between S1 and S3, 7,696 between S2 and S3. Additionally, DEGs were mainly enriched in metabolism and signal transduction pathways.