Transcriptome and proteome of yak oocytes in vitro maturation
The aim of this study was to investigate the molecular regulation mechanism of yak oocytes during in vitro maturation. Oocytes at the GV and MII stages were collected for RNA-seq and iTRAQ sequencing. The analysis identified 7999 differentially expressed genes and 353 differentially expressed proteins,including 4706 and 219 up-regulated genes and proteins, 3293 and 134 down-regulated genes and proteins. Transcriptomics and proteomics analysis showed that GO functional classification annotated biological processes such as ' cellular processes ' were closely related, and molecular events such as ' binding ' molecular function-related categories were active. KOG analysis indicated that oocytes had complex needs for material metabolism and genetic material processing during maturation. KEGG pathway analysis showed enrichment of pathways such as MAPK signaling, Rap1 signaling, Regulation of actin cytoskeleton, Progesterone-mediated oocyte maturation, Ferroptosis, TGF-beta signaling, and PPAR signaling during oocyte maturation. This study provides abundant data for further analysis of the molecular regulation mechanism of yak oocyte maturation and the specificity of yak reproduction.