A novel CRISPR/Cas9 screening platform identifies an IRF1-SOCS1-mediated negative feedback loop that limits CXCL9 expression and anti-tumor immunity (CRISPR screen)
Investigation and validation of genes that regulate CXCL9 expression through a genome-wide in vitro CRISPR/Cas9 screen. Overall design: AT-3 cells containing a GFP reporter for CXCL9 were transduced with a genome wide CRISPR library and stimulated with IFNg and TNF.