Transcriptional and Metabolic Adaptations of Bifidobacterium breve GZX43 Under Nutrient Limitation Conditions
Bifidobacteria are important members of the gut microbiota, widely studied for their probiotic properties and applications in the food industry. The metabolic characteristics of bifidobacteria may be influenced by environmental changes, particularly variations in nutrient availability. To better understand the metabolic adaptation mechanisms of Bifidobacterium breve under nutrient-limited conditions, we developed a chemically defined medium (CDM) suitable for the cultivation of multiple bifidobacterial strains. Using RNA-seq analysis and metabolite validation, we explored the metabolic changes of B. breve GZX43 under 1% glucose CDM nutrient limitation, covering the logarithmic, stationary, and death phases.