Metagenome of the spleen of largemouth bass (Micropterus salmoides)
The sampling location was Wuxi, China. A total of 200 largemouth bass were randomly selected for a challenge experiment. Mortality occurred between 12 and 24 hours post infection, and the survival time of each fish was recorded. To minimize errors related to injection procedures, individuals that died within 48 hours post challenge were not sampled. Death was defined as no activity, no opercular movement, and no response to stimuli. During the peak mortality period between 48 and 72 hours, spleen tissues from moribund fish were collected to form the susceptible group SS, while spleen tissues from individuals that survived for more than 5 weeks without obvious clinical symptoms were assigned to the resistant group SR. On day 3 and day 35 post infection, six spleen tissue samples were aseptically collected from the SS group and the SR group, respectively. These samples were then pooled in pairs to obtain three pooled samples per group, which were used for ATAC assays.