Effect of metformin in the mouse ileum gene expression
Metformin is first line treatment for type 2 diabetes, however its exact mechanisms remain incompletely understood. Furthermore, the role of glucose transporter in intestine are highlighted to investigate metabolic change. We here report RNA change after metformin treatment in ileum tissue. We are going to focus on change of glucose transporter and relate factor of those changes. In previous study metformin increases expression of GLUT1/GLUT2 in ileum and metformin plays role as complex1 inhibitor so we are going to find target of metformin-induced glucose metabolic increasment Overall design: To investigate metformin-induced changes in ileum, we prepare 2 group that orally gaveaged vehicle or metformin 300mg/kg daily/6days after 6days we gave 600mg/kg orally treatment lastly before sacrifice. 2hours after last treatment gavaged mouse sacrificed and ileum tissue collected. Each group has 3 animals and total RNA has extracted by each tissue.