Detection of ToLCNDV resistance in cucumber through agroinoculation under controlled condition
In this study, Agrobacterium tumefaciens containing recombinant plasmids were used to agroinoculate cucumber seedlings with tandem repeats of DNA-A genome of Tomato leaf curl New Delhi virus (ToLCNDV). The inoculated seedlings, representing both resistant and susceptible accessions, were monitored for symptoms over a period of four weeks. Symptom development was observed in susceptible genotypes, while the resistant genotype remained symptomless. Leaf samples were collected from both agroinoculated and control plants for further analysis. This research sheds light on the pathogenicity of ToLCNDV in cucumber and highlights the potential resistance mechanisms in certain accessions. Overall design: Uppermost leaves of each cucumber seedlings were agroinoculated with 20 µl of Agrobacterium culture mixture followed by pricking with needle. Plants uninoclated with ToLCNDV was assumed as control plants. P1C - Resistant Control P1V - Resistant Virus inoculated P2C - Susceptible Control P2V -Susceptible Virus inoculated