Short-term effects of overexpression of GK and/or GKRP on the transcriptome in mouse primary hepatocytes
Short-term effects of overexpression of GK and/or GKRP on the transcriptome in mouse primary hepatocytes Overall design: GKRP-deficient hepatocytes from female GCKR-P446L-EMI-B6N mice (n=3: LL; LL; LP) were cultured in monolayer and treated with adenoviral vectors for GK or GKRP(446P or 446L) expression Sequences for GKRP vectors were Human: BC130481; Mouse: XM_006503883; GK PMID: 8550593. After 24h-culture in MEM with 5mM glucose, they were incubated for 4h in medium containing 25mM glucose and then harvested for RNA extraction and sequencing