Human gut and salivary microbiota before, during, and after fructooligosaccharide supplementation
Feces and salivary samples were collected from 11 healthy Japanese adults. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R) and multiplexed amplicon pyrosequencing was carried out using Roche 454 GS FLX Titanium or 454 GS JUNIOR sequencing system. Whole metagenomic shotgun sequencing was performed for fecal samples using Illumina NovaSeq 6000 S4 flow cell (2 Ã? 150bp).