Transcriptome defense response of orange fruit Induced with Rhodoturola mucilaginosa enhanced with salicylic acid
Purpose: The goal of this study is to looked at the expression levels of defense genes in orange fruit in response to the application of the antagonist R. mucilaginosa enhanced with SA Methods: Three identical and evenly distributed wounds (5 mm diameter and 3 mm deep) made at the equator of each fruit were inoculated with 30 µL cell suspension of R. mucilaginosa (1×108 cells/mL) + 0.2 mM SA, whiles cell suspension of R. mucilaginosa alone (1×108 cells/mL) was used as a control. Treated fruits were then kept in plastic baskets and covered with film wraps to maintain relative humidity of 95% at storage temperature of 20 ºC. After 72 h of storage the wound tissues were excise and immediately kept in liquid nitrogen and maintained at -80 ºC for the analysis of the RNA. There were three replications per each sample. Results: The results showed that 46 genes were identified as DEGs. Among these 34 were up-regulated and 12 were down regulated, 10 of the RNA-seq these were validated using qRT-PCR. RNA-seq data had a linear relationship withqRT_PCR with a corelation coefficent value of (R2) 0.8388. Conclusion: Our finding showed that the defense genes of orange fruit were up-regulated by the innoculation of R. mucilaginosa enhanced with 0.2 mM SA in the fruit. Overall design: Examine expression levels of defense genes in orange fruit in response to the application of the antagonist R. mucilaginosa enhanced with SA