Transcriptomic profiling of pancreatic stellate cells and pancreatic cancer cells in a gemcitabine-driven tumor-stroma supernatant transfer model
Pancreatic ductal adenocarcinoma (PDAC) is characterized by extensive tumor-stroma interactions that influence disease progression and therapy response. To investigate transcriptional changes associated with tumor-stroma communication following chemotherapy, we performed transcriptomic profiling in a supernatant transfer system.Murine pancreatic cancer cells (KPC) were treated with the chemotherapeutic agent gemcitabine, and supernatants were collected from treated or control tumor cells. Pancreatic stellate cells (PSCs) were exposed to these tumor cell-derived supernatants. Subsequently, supernatants from PSCs previously exposed to tumor cell supernatants were transferred to pancreatic cancer cells. RNA sequencing was performed to characterize gene expression changes associated with chemotherapy-induced tumor-stroma signaling.