Bulk RNA sequencing of bovine (cow) lung cells from uninfected, and infected cells (BCG and M. tuberculosis).
Whole RNA transcriptome were performed on bovine lung cell 3D pulmosphere cultures with or without infection with M. bovis BCG and M. tuberculosis H37Rv strain. Overall design: Pulmospheres were cultured in U-bottom 96 well plates with or without infection. 24 hours post infection pulmospheres were harvested, total RNA were extracted and subjected to paired-end RNA sequencing.