Genome-wide RNA-Seq analysis of mRNAs level under the action of Pyr-Arg-Pro peptide in the norm and acute stress conditions
White 2-month-old male rats of the Wistar line (weight, 200-250 g) were used. The animals were divided into the "health after water administration" (CW), "health after Pyr-Arg-Pro administration" (CY), "stress after water administration" (SW), and "stress after Pyr-Arg-Pro administration" (SY). Each group contained 3 animals. To rats of the CY and SY groups, peptide Pyr-Arg-Pro was administered intraperitoneally at a dose of 150 microg/kg. To rats of the CW and SW groups, sterile water was administered intraperitoneally at a dose of 1 ml/kg. Acute stress was caused by the immobilization of animals, as well as light and acoustic exposure for 1 h at 30 min after injection (SW and SY groups). Also, all animals were tested using Elevated Plus-maze (within 5 min at 3 h after injection) and Elevated Zero-maze (within 5 min at 5.5 h after stress). Rats were withdrawn from the experiment at 6 h after injection. mRNA fraction from total RNA of rat hippocampus was used for high-throughput RNA-sequencing (RNA-Seq). Sequencing was carried out using an Illumina HiSeq 1500 instrument. At least 10 million reads (1/50 nt) were generated.