In vivo CRISPR screens for Toxoplasma using metabolism sublibrary in WT and Ifngr1-deficient  mice
We performed in vivo CRISPR screen of Toxoplasma targeting metabolism-related genes to identify in vivo fitness genes in WT and Ifngr1-deficient mice. Overall design: This sublibrary targeting 361 genes which consisted 3610 gRNAs targeting metabolism-related genes together with dispensable and essential control genes. We performed in vivo CRISPR screens to identify novel virulence factors of Toxoplasma.