Mus musculus Transcriptome or Gene expression
This study aimed to investigate gene expression changes and intercellular communication between cumulus cells (CCs) and mural granulosa cells (MGCs) during ovulation in mice. RNA-sequencing was conducted at 0, 4, and 12 hours after hCG administration. In addition to differential gene expression analysis, we performed interactome analysis to infer signaling pathways and ligand-receptor interactions between CCs and MGCs. The results provide insights into the molecular and paracrine mechanisms regulating ovulation.