Multi-omics analysis of ruminal microbiota and transcriptome related to milk pentadecanoic acid (C15:0) synthesis in Chinese Holstein dairy cows (PRJCA049216)
This project investigates the regulatory mechanisms of milk pentadecanoic acid (C15:0) synthesis in Chinese Holstein dairy cows using microbiome and transcriptome approaches. Ruminal microbiota were profiled by 16S rRNA sequencing of ruminal fluid samples, and bovine mammary epithelial cells (BMECs) were subjected to RNA-seq after treatment with propionylcarnitine (PLC). The datasets include raw sequencing data generated on the Illumina NovaSeq 6000 platform.