Single-cell transcriptomic analysis of plant quiescent center by third-generation sequencing reveals developmental trajectories (PRJCA045162)
We performed transcriptional profiling of the Arabidopsis thaliana quiescent center (QC) across two developmental stages and investigated its dynamic transcriptome remodeling by Third-Generation Sequencing (TGS). We revealed that genes involved in cell growth, auxin response, and cell division process regulating root development. QC marker genes TERMINAL EAR1-like (TEL1) participated in positive regulation of the sustain of root tip stem cells by WUSCHELRELATED HOMEOBOX 5 (WOX5). Finally, rice QC cells were isolated sequenced and found divergent in auxin biosynthesis, transport, and signaling pathways from Arabidopsis thaliana.