Macrobrachium rosenbergii Ovarian Redevelopment
Transcriptome profiling of the hepatopancreas and ovarian tissues across key developmental stages of Macrobrachium rosenbergii ovarian redevelopment was performed to elucidate the molecular regulatory mechanisms and tissue-specific interaction between hepatopancreas and ovary during this process. Ovarian tissues (OV I, OV III, OV V ) and matched hepatopancreas tissues (HP I, HP III, HP V) of healthy female Macrobrachium rosenbergii at different ovarian redevelopment stages were collected with three biological replicates. Total RNA was extracted, and strand-specific cDNA libraries were constructed and sequenced on the Illumina NovaSeq 6000 platform with paired-end 150 bp reads. Raw and processed transcriptome data were generated, including differential expression analysis and functional enrichment analysis. Key pathways associated with ovarian redevelopment such as ribosome biogenesis and RNA polymerase, and key genes including Vg, Vgr and Cdc42 were identified. This dataset provides fundamental molecular resources for investigating crustacean ovarian development and reproductive regulation.