Single-cell RNA sequencing of Versican (Vcan) knockout mice after cardiac ischemia.
The goal of this experiment was to profile the cellular response to myocardial infarction in Versican (Vcan) deficient mice. Cardiac interstitial cells were isolated from WT and Vcan-KO mice 7 days after cardiac ischemia with reperfusion. Single, living, CD31- CD45- cells were sorted by FACS and used as input for single-cell RNA sequencing using the 10X Genomics Chromium platform.
The goal of this experiment was to profile the cellular response to myocardial infarction in Versican (Vcan) deficient mice. Cardiac interstitial cells were isolated from WT and Vcan-KO mice 7 days after cardiac ischemia with reperfusion. Single, living, CD31- CD45- cells were sorted by FACS and used as input for single-cell RNA sequencing using the 10X Genomics Chromium platform.