Whole-genome sequencing of outbred Sprague-Dawley rats tested for addiction-relevant behavioral traits
This BioProject contains whole-genome sequencing data from commercially outbred Charles River Sprague-Dawley rats used to study common genetic variation associated with addiction-relevant behavioral traits. Rats were phenotyped for locomotor response to a novel environment, elevated plus maze behavior, and tail flick thermal nociception. High molecular weight genomic DNA was isolated from spleen samples at the University of Michigan and transferred to the University of California San Diego for sequencing and genetic analysis. Low-coverage whole-genome sequencing was performed for 654 rats to enable genotype inference and genome-wide association studies. Eight of these rats were also deeply sequenced to approximately 19x as a truth set to assess genotype accuracy.