Transcriptome analysis of pepper under salt treatment (PRJCA049200)
Salt stress was applied by adding sodium chloride (NaCl) to the nutrient solution to reach a final concentration of 200 mM, while control plants received only the nutrient solution. Root tissues from both salt-treated and control plants were collected at 0, 0.5, 1, 3, 6, 12, and 24 hours after treatment. To minimize circadian rhythm interference on gene expression, the sampling schedule was carefully arranged as follows: 8:00, 8:30, 9:00, 11:00, 14:00, and 20:00 on the first day, and 8:00 on the following day for both treated and control plants.