OsRqc1 regulates the CSIT in tms5-containing TGMS lines by recruiting OsVms1 to 60S ribosomal subunit (PRJCA035395)
Here, we characterized a CSIT related gene OsRqc1, which is a member of ribosome-associated quality control (RQC). We found OsRqc1 binds 60S ribosomal subunit and participates in RQC, regulates CSIT of tms5 lines by recruiting OsVms1 to 60S ribosomal subunit to generate cP RNAs. We further determined that the variation of 6-bp repeat within 5UTR of OsRqc1 among rice subspecies affects its transcriptional level, thereby regulating the CSIT of tms5 lines. These findings not only reveal the mechanism of OsRqc1 OsVms1 module regulating CSIT, but also provide valuable insight into the variation of OsRqc1 for molecular design breeding of two-line hybrid rice tms5 lines.