Mus musculus strain:C57BL/6J Raw sequence reads
Mutations in PAX5 account for 30% of B cell acute lymphoblastic leukemia (B-ALL) cases. Recently, two subtypes of PAX5-driven B-ALL have been defined. The first group is characterized by the prototypic pPro80Arg mutation while the other contains a broad range of PAX5 alterations (PAX5alt). Here we provide single cell RNA sequencing data the first mouse model for the PAX5alt subtype of B-ALL caused by a premature STOP mutation (Pax5Y351*).B cells isolated from the bone marrow and spleen of Pax5wt/wt and Pax5Y351*/Y351* mice From the bone marrow of Pax5Y351*/Y351* mice, we sorted both the unique CD19+B220- B cell population as well as the CD19+B220+ cells found in both wildtype and mutant mice. In the spleen, we sorted total CD19+ B cells and disregarded B220 expression, as B220 was heterogeneously expressed among the splenic B cell population. After sorting, single cell RNA seq libraries were prepared using the Single Cell 5' v3 V(D)J library preparation from 10X Genomics. Samples sequenced on the same were index using hashtag oligos. Data was processed using cell ranger with default settings. Processes data can be found in the supplementary section of the associated manuscript.