Capture-Hi-C targeting the H2Aub regions in Arabidopsis (PRJCA036720)
The Polycomb group complexes are well known for gene repression through the incorporation of H2Aub and H3K27me3, as well as organizing the chromatin 3D structure. However, in Arabidopsis, H2Aub and H3K27me3 do not overlap so well, and only H2Aub regions exist. H3K27me3 is confirmed to contribute to establishing repressive chromatin loops and other repressive chromatin 3D structures. It is unclear whether only H2Aub regions are also separate from H3K27me3 in space. In addition, comparing with PRC2/H3K27me3, little is known about PRC1/H2Aub on chromatin folding and its role in transcription regulation. Here, we conduct Capture-Hi-C to specifically enrich the interactions related to H2Aub enriched regions (H2Aub loops).