Landscape transcriptomic analysis of bovine follicular cells during key phases of ovarian follicular development
Our study investigated the transcriptional signature of follicular fluid cell populations during ovarian follicular development in dairy cows. We controlled follicular development in vivo and collected follicular cells at specific phases (pre-deviation, deviation, post-deviation, and preovulatory) to observe gene expression patterns. Our findings revealed an upregulation of genes associated with mitochondrial viability, cell proliferation, apoptosis prevention, and steroidogenesis stimulation in the future dominant follicle, contrasting with the upregulation of genes related to reduced viability and cell death in the non-selected follicle.