Transcriptomic profiling of microglial polarization modulated by Delphinidin-3-O-glucoside in a hypoxic-ischemic encephalopathy model (PRJCA051706)
This project presents transcriptomic data from BV2 microglial cells subjected to oxygen-glucose deprivation (OGD) to mimic hypoxic-ischemic injury, with or without treatment with Delphinidin-3-O-glucoside (Dp3G), a bioactive anthocyanin. The study aims to elucidate the molecular mechanisms by which Dp3G reprograms microglial polarization from a pro-inflammatory M1 phenotype to an anti-inflammatory M2 phenotype, thereby exerting neuroprotective effects in hypoxic-ischemic encephalopathy (HIE).RNA sequencing was performed on three experimental groups: control, OGD, and OGD + Dp3G. Differential expression analysis revealed key pathways involved in immune regulation and metabolic reprogramming, including oxidative phosphorylation and NF-?B signaling. Key upregulated genes such as NLRC3 and ACOD1 were identified, suggesting their roles in mediating the anti-inflammatory and metabolic effects of Dp3G.These data provide a resource for understanding how natural compounds like Dp3G modulate microglial function and offer potential therapeutic targets for neonatal HIE and other neuroinflammatory disorders.