RNA sequencing of BV2 cells induced by ferroptosis. (PRJCA020474)
To  investigate the changes in gene expression of glial cells underferroptosis conditions and the regulation of LPC, we selected the microglial cell line BV2. The cells were induced to undergo ferroptosis using the inducer RSL3, and LPC was added to observe its protective effect on the cells. The experiment was divided into three groups: the control group, which was treated with 1% MDSO and 1% PBS; the iron death induction group, which was treated with 7 µM RSL3; and the LPC treatment group, which was treated with 7 µM RSL3 and 500 nM LPC.
To  investigate the changes in gene expression of glial cells underferroptosis conditions and the regulation of LPC, we selected the microglial cell line BV2. The cells were induced to undergo ferroptosis using the inducer RSL3, and LPC was added to observe its protective effect on the cells. The experiment was divided into three groups: the control group, which was treated with 1% MDSO and 1% PBS; the iron death induction group, which was treated with 7 µM RSL3; and the LPC treatment group, which was treated with 7 µM RSL3 and 500 nM LPC.