The miRNA + mRNA dataset from the intermittent fasting NAFLD model (PRJCA041477)
We investigated the therapeutic potential of intermittent fasting (IF) in non-alcoholic fatty liver disease (NAFLD) using mouse models and aimed to identify novel biomarkers. First, we established four groups of male C57BL/6J mice (8 weeks old, n=5 per group): chow ad libitum (CD-AL), chow with every-other-day fasting (CD-EODF), high-fat diet ad libitum (HFD-AL), and high-fat diet with every-other-day fasting (HFD-EODF). After 12 weeks on their respective diets, the EODF groups underwent 40 days of alternating 24-hour feeding and 24-hour fasting. Mice were anesthetized with isoflurane, and liver tissues were collected for mRNA and miRNA sequencing. In the second model, male C57BL/6J mice (8 weeks old, n=8 per group) were fed a high-fat diet for 12 weeks. Mice were injected via tail vein with either AAV-control or AAV-miR-532-5p sponge virus to knock down miR-532-5p specifically in the liver. After 12 weeks, mice were anesthetized and liver tissues were harvested for mRNA sequencing.