Identification of Or5v1/Olfr110 as an oxylipin receptor and anti-obesity target (PRJCA048617)
The hepatocytes were isolated from WT and Olfr110-/- mice that were administrated with control vehicle or 12(S)-HEPE (0.1 mg/g, i.p.) every day for one week. Total RNA of hepatocytes was extracted using TRIzol reagent (Invitrogen), according to manufacturer' s procedure, quantified and quality controlled using a 2100 Bioanalyzer (Agilent). RNA samples with RNA Integrity Number (RIN) above 7 were depleted for ribosomal RNA and converted into cDNA library.