Ent-kaurane diterpenoids isolated from Siegesbeckia orientalis L. suppress methicillin-resistant Staphylococcus aureus (PRJCA039185)
The present study investigates the anti-MRSA activity of four ent-kaurane-type diterpenoids isolated from the ethyl acetate extract of Siegesbeckia orientalis L.: 16ß-hydro-ent-kauran-17,19-dioic acid (1), 16a,17-dihydroxy-ent-kauran-19-oic acid (2), 16ß,17,18-trihydroxy-ent-kauran-19-oic acid (3), and 17,18-dihydroxy-ent-kauran-19-oic acid (4). Transcriptomic analyses indicated that Compound 1 disrupts the integrity of the cell wall and cytoplasmic membrane by impairing peptidoglycan synthesis, altering the metabolism of cell wall precursors, and modulating the expression of genes associated with ion transport and membrane-related metabolic enzymes.