Histone lactylation is involved in mouse oocyte maturation and embryo development (PRJCA021062)
To investigate the influence of sodium lactate on enhancing histone lactylation during oocyte maturation, the base medium was Opti-MEM supplemented with 4 mg of BSA. Sodium lactate solution was added with various concentrations (0 mM and 10 mM,). To ensure adequate sodium lactate uptake, oocytes were initially cultured with 3-isobutyl-1-methylxanthine (IBMX, phosphodiesterase inhibitor) and sodium lactate for 24 hours, after which they were transferred to the same concentration medium without IBMX for continued culture.