Phyllanthin Targets GBP5 to Suppress Macrophage M1 Polarization and Alleviate DSS-Induced Colitis in a Murine Model (PRJCA049425)
This research investigates Phyllanthin's anti-colitis mechanism through macrophage polarization regulation. Transcriptomic analysis compared M0, LPS/IFN-gamma-induced M1, and PHY-treated M1 RAW264.7 macrophages (n=5). Libraries from polyA-selected RNA were sequenced on Illumina NovaSeq 6000 (PE150), generating raw FASTQ data. Results demonstrate Phyllanthin reverses pro-inflammatory M1 transcriptional signatures by targeting GBP5, revealing its therapeutic mechanism against experimental colitis.