Transcriptome Analysis of MfERF053 Lines with Three Genetic Backgrounds (RNAi/WT/OE) in Roots and Leaves under Natural Drought Stress (PRJCA048240)
This project takes three lines of the MfERF053 gene, namely gene silencing (RNAi), wild type (WT), and overexpression (OE), as research materials, and conducts natural drought stress experiments after the lines grow uniformly. Samples are collected and named following the strategy of stress stage (before stress, the 4th day after natural drought stress) + line type + tissue part (root, leaf) (for example, RNAi-L refers to the leaf of the RNAi line before stress, RNAi-LD refers to the leaf of the RNAi line on the 4th day after stress; similarly, root tissues such as RNAi-R and RNAi-RD are included, with a total of 12 types of samples). Through transcriptome analysis of these samples, the study aims to identify functional pathways involved in the drought resistance process, reveal the gene regulatory networks and signaling pathways related to MfERF053, and explore downstream core target genes, thereby providing key evidence for elucidating the molecular mechanism of plant drought resistance mediated by MfERF053.