Transcriptome-wide m6A landscape across diverse porcine tissues revealed by Nanopore direct RNA sequencing (PRJCA048068)
In summary, we employed Nanopore DRS to conduct the first comprehensive epitranscriptomic profiling of m6A modifications across diverse porcine tissues. We identified 60,823 transcripts, including 27,823 novel isoforms, and detected 343,951 m6A sites with distinct abundance between adult (80,336 sites) and fetal (92,476 sites) stages. Our analysis revealed substantial m6A heterogeneity across tissues, with brain regions exhibiting particularly pronounced specificity, and revealed sig-nificant associations between m6A methylation and AS regulation. This work pro-vides new insights into the regulatory complexity of the porcine transcriptome and epitranscriptome, establishing a valuable resource for advancing genetic and disease modeling research in pigs
In summary, we employed Nanopore DRS to conduct the first comprehensive epitranscriptomic profiling of m6A modifications across diverse porcine tissues. We identified 60,823 transcripts, including 27,823 novel isoforms, and detected 343,951 m6A sites with distinct abundance between adult (80,336 sites) and fetal (92,476 sites) stages. Our analysis revealed substantial m6A heterogeneity across tissues, with brain regions exhibiting particularly pronounced specificity, and revealed sig-nificant associations between m6A methylation and AS regulation. This work pro-vides new insights into the regulatory complexity of the porcine transcriptome and epitranscriptome, establishing a valuable resource for advancing genetic and disease modeling research in pigs