GlycoRNA profiling in PK-15 cells during Pseudorabies virus infection (PRJCA050136)
This study investigates glycoRNA expression dynamics in PK-15 cells following Pseudorabies virus (PRV) infection using the rPAL (periodate oxidation and aldehyde labeling) method for glycoRNA-specific enrichment. The approach leverages periodate oxidation of cis-diol groups in glycoRNA sugar chains, followed by biotin conjugation and streptavidin-based purification. Comparative analysis of input and enriched libraries revealed significant alterations in host  glycoRNAs across infection, with notable enrichment of specific non-coding RNA species. This work provides comprehensive insights into viral-induced modifications of the glycoRNA landscape.