Construction of MDBK Genome-Wide Mutant Library and Screening of BPIV-3 Susceptible Genes (PRJCA048757)
In this study, a genome-wide mutant library was constructed using CRISPR/Cas9 gene editing technology based on bovine kidney cells. Using this library, we performed a BPIV-3 challenge screen, followed by sequencing analysis of the surviving cells to identify BPIV-3-susceptible genes.