m6A-seq analysis of Pyrus fruit wounded and inoculated with P. expansum
This project investigates m6A RNA methylation in pear (Pyrus spp.) fruit under wound stress and inoculation with the fungal pathogen Penicillium expansum. Three uniform artificial wounds (5 mm diameter and depth) were inflicted along the equator of each fruit. The treatment group (T3) received 30 uL of P. expansum spore suspension per wound, while the control group (CK2) was mock-inoculated with sterile water. Samples were collected 36 hours post-inoculation, flash-frozen, and stored at -80 C prior to RNA extraction. Both IP-enriched and input control libraries were prepared for high-throughput m6A-seq on the Illumina platform to analyze transcriptome-wide RNA methylation patterns. Three independent biological replicates were included for each condition.