The molecular mechanism of endogenous ROS and metabolic disturbance in individual rice plants induced by GCR heavy ions in space environment (PRJCA021425)
In order to obtain the individuals hit by heavy ions with different LET values, Chinese Shijian-10 recoverable satellite (SJ-10) was utilized to fly the sandwich biostack for detecting heavy ion radiation, which was formed by plastic nuclear track detectors (CR-39) and rice seeds, in a low Earth orbit (LEO) of 252 km for 12.5 days. By detecting the tracks on the CR39 in close contact with the embryos of the rice seeds, the LET values were obtained. The botanical traits of rice plants at different development stages when exposed to heavy ions with different LET values were analyzed, and the ROS species in the root tip tissues were detected. Transcriptome sequencing were conducted on seven LET group (Table 1) samples of tillering and heading stages, with each LET group containing three biological replicates (with similar LET values). Moreover, to study the relationship between the individual methylation and the expression of methyltransferase after the heavy ion hit its seed embryo, methylation sequencing was also performed on CR-39 penetrated plants (these individual plants were broken down in the plateau region of the heavy ion energy release) : two plants from the R60 group (R60-2, R60-3), two plants from the R100 group (R100-2, R100-3), one plant from the R150 group (R150-1), and two plants from the R200 group (R200-1, R200-2) separately. Three from the CK group (CK-1, CK-2, CK-3). And both the transcriptome sequencing and the methylation sequencing were performed on the same third tillering leaf of the same plant.