GLTSCR1-KO samples of HCT116 cells
Here, we identify GLTSCR1 as a regulator of NHEJ pathway. Deficient of GLTSCR1 causes resistance to DNA damage in colorectal cancer (CRC) cells through promoting NHEJ repair efficiency. Mechanistically, GLTSCR1 inhibits ubiquitination degradation of BRD9 by combining with BRD9 to constitute two unique subunits of non-canonical BAF complex (GBAF). Thus, GLTSCR1 deficiency aberrant opening the promoter region of NHEJ repair associated genes. This study highlights the molecular mechanism of the GLTSCR1 - BRD9 interaction in chromatin remodeling regulation, which provides a potential therapeutic target for CRC.