Prostaglandin E2-driven dedifferentiation of Schwann cells leads to perineural infiltration in pancreatic ductal adenocarcinoma (PRJCA049289)
This project uses the spontaneously immortalized rat Schwann cell line (RSC96 cells, species: Rattus norvegicus) as the research model. Through transcriptome profiling (e.g., RNA sequencing) and functional experiments, it explores key scientific issues in the context of perineural invasion (PNI) in pancreatic ductal adenocarcinoma (PDAC), including: the expression changes of dedifferentiation markers (c-Jun, Sox2, GDNF) and functional molecules (LIF, ADAMTS-1) when RSC96 cells interact with PDAC cells (PANC-1, BxPC-3); the impact of prostaglandin E2 (PGE2) stimulation on the morphology and migration dynamics of RSC96 cells in the pancreatic cancer microenvironment; and the role of RSC96 cells in extracellular matrix (ECM) degradation and neural remodeling during PDAC-PNI. Ultimately, the project provides comprehensive data resources for studying the interaction between Schwann cells and tumor cells, identifies potential therapeutic targets for PDAC-PNI (such as PTGES and PGE2), and lays a foundation for the development of therapies related to pancreatic cancer perineural invasion.