The global increase in DNA methylation during recalcitrant Panax notoginseng seeds after-ripening process (PRJCA017344)
To investigate the epigenetic regulation of recalcitrant seeds after-ripening and germination, we characterized the DNA methylomes, genome-wide siRNA profiles, and transcriptomes of embryo and endosperm in P. notoginseng seeds at different after-ripening stages. We observed a global DNA methylation increase in germinate seeds compared to dormant seeds, similar to that observed during sweet orange ripening. The application of a DNA methylation inhibitor caused hypomethylation and early germination, suggesting that the ripening-induced increase in DNA methylation is important for recalcitrant seeds after ripening.