Single-cell transcriptome profiling of immunized chicken reveals the cellular mechanism of B-cell activation and antibody secretion (PRJCA049782)
This study presents a single-cell transcriptomic atlas of chicken peripheral blood following immunization with heat-inactivated S. epidermidis. Using flow cytometry and 10x Genomics, we mapped the major peripheral immune cell types, including B cells, T cells, macrophages, and thrombocytes. We tracked B-cell maturation trajectories and dissected ligand-receptor signaling networks underlying IgY affinity maturation.