Metagenomic Sequencing of Tobacco Root-Zone Soil from Healthy and Bacterial Wilt-Diseased Fields
This study aims to investigate the differences in microbial community composition and functional potential in tobacco rhizosphere soil between healthy fields and fields severely affected by bacterial wilt (caused by Ralstonia solanacearum). Soil samples were collected from the root zone of tobacco plants in Guizhou, China. Metagenomic DNA was extracted and subjected to shotgun metagenomic sequencing. The resulting sequences will be used for taxonomic profiling, functional annotation, and comparative analysis to explore the microbial mechanisms associated with disease suppression or susceptibility.