Morphological and gene expression changes in Nematostella vectensis embryos induced by hypoxia
We examined three developmental stages of Nematostella vectensis embryos. Embryos at each stage were exposed to normoxic or hypoxic conditions for a total of 12 hours. The treatment schemes were as follows: 18 hpf embryos exposed to hypoxia for 12 hours starting at 6 hpf (6N+12H), 24 hpf embryos exposed to hypoxia for 12 hours starting at 12 hpf (12N+12H), and 36 hpf embryos exposed to hypoxia for 12 hours starting at 24 hpf (24N+12H). Total RNA was extracted from three biological replicates per condition (hypoxia and normoxia) at each developmental stage. Library preparation was performed using Illumina poly(A) RNA purification, and sequencing was conducted on the NovaSeq 6000 platform.