A single-cell transcriptomic dataset profiling traumatic brain injury and NeuroD1-based gene therapy in mice (PRJCA048209)
To elucidate the molecular and cellular mechanisms involved, we conducted single-cell RNA sequencing using controlled cortical impact mice of TBI to compare the effects of vehicle treatment with those of NeuroD1-based gene therapy. Single-cell RNA sequencing reveals significant shifts in cellular composition and astrocyte subtypes. These changes involve upregulated synaptic assembly and myelination pathways alongside downregulated mitochondrial and metabolic functions in TBI mice.
To elucidate the molecular and cellular mechanisms involved, we conducted single-cell RNA sequencing using controlled cortical impact mice of TBI to compare the effects of vehicle treatment with those of NeuroD1-based gene therapy. Single-cell RNA sequencing reveals significant shifts in cellular composition and astrocyte subtypes. These changes involve upregulated synaptic assembly and myelination pathways alongside downregulated mitochondrial and metabolic functions in TBI mice.