16S rRNA and ITS amplicon sequencing of microbial communities in ecological restoration area of iron tailings
This BioProject contains 16S rRNA and ITS amplicon sequencing data of microbial communities in different ecological restoration areas of iron tailings. The main objectives are to explore the composition, diversity, and functional characteristics of bacterial and fungal communities during the restoration process, and to reveal the microbial ecological mechanisms involved in the reconstruction of tailings soil functions. Soil samples were collected from different restoration plots with different vegetation types and restoration ages. Genomic DNA was extracted from each sample, and the V3-V4 region of the 16S rRNA gene (for bacteria) and the ITS1 region (for fungi) were amplified and sequenced using the Illumina MiSeq platform. The resulting sequence data will be deposited in the NCBI Sequence Read Archive (SRA) to support further analysis of microbial community succession and ecological restoration assessment in iron tailings ecosystems.