Duodenal mucosal electroporation ablation in type 2 diabetic mice: transcriptomic and metagenomic profiling
Irreversible electroporation (IRE)-based duodenal mucosal electroporation ablation (DMEA) is an emerging endoscopic intervention for type 2 diabetes mellitus (T2DM). To investigate its underlying mechanisms, we performed DMEA or sham surgery in male db/db mice. This BioProject includes two complementary sequencing datasets: 1. Bulk RNA-seq of duodenal mucosal tissues (n=9 per group, Sham vs. DMEA) to identify differentially expressed genes and enriched pathways. 2. Metagenomic shotgun sequencing of cecal content (n=9 per group, Sham vs. DMEA) to characterize changes in gut microbial composition, diversity, and functional potential. All mice were maintained under specific pathogen-free conditions. Tissues were collected 6 weeks post-intervention. RNA-seq libraries were prepared using poly-A enrichment and sequenced on an Illumina NovaSeq 6000 platform (paired-end 150 bp). Metagenomic libraries were prepared using the NEBNext Ultra DNA Library Prep Kit and sequenced on the same platform (paired-end 150 bp, target depth 5 Gb per sample).