Integrative Analysis of Transcriptome and ChIP-seq Reveals the Function of DMRT2 in Chicken Gonadal Development
This project aims to elucidate the molecular mechanisms by which DMRT2 regulates gonadal development and sexual differentiation in the chicken (Gallus gallus). The study comprises two complementary high-throughput sequencing datasets: 1. Transcriptome Analysis (RNA-seq): RNA sequencing was performed to investigate the transcriptional landscape following DMRT2 overexpression in embryonic female chicken gonadal cells at embryonic day 12 (E12). This dataset enables the identification of downstream target genes and signaling pathways modulated by DMRT2. 2. Chromatin immunoprecipitation sequencing(ChIP-seq): ChIP-seq was conducted on both male and female chicken embryonic gonads at E18 to map genome-wide DMRT2 binding sites. This analysis serves to identify direct transcriptional targets of DMRT2 in vivo. All data were generated from Gallus gallus embryonic gonad samples. This comprehensive genomic resource is publicly released to support and advance research in avian sex determination, reproductive biology, and comparative genomics.