micro-RNA sequencing of Dendritic cell derived exosomal (PRJCA035314)
Glioma is the most aggressive and malignant primary brain tumor with poor prognosis. The antitumor immunity induced by Photodynamic therapy (PDT) is one of the important mechanisms of its efficacy. Dendritic cell (DC) derived exosomes (Dexs) have been proven to play an important role in tumor antigen presentation and activation of killer T cells. The identification of the role and regulatory mechanism of Dexs in photodynamically induced anti-tumor immunity is expected to further enhance the efficacy of PDT. GL261 cells were irradiated by PDT first, and after co-culture with DCs, exosomes (PDT-Dexs) of DC culture supernatant were finally obtained. The effects of PDT-Dexs on DC maturity and T cell activation were measured by flow cytometry and Elisa. MicroRNA sequencing was performed on PDT-Dexs and Control groups. Finally, the effects of PDT-Dexs and combined PDT treatment on tumor growth and survival of glioma model mice were observed using immune healthy mice and immune deficient mice. PDT-Dexs extracted was co-incubated with DCs, and the maturity of DCs was significantly increased. The co-incubation of PDT-Dexs with T cells can significantly promote the activation of T cells. The microRNA sequencing results showed that 253 micrornas were significantly up-regulated in PDT-Dexs compared with the control group. KEGG and GO analysis showed that the differential genes were mainly concentrated in the PI3K-Akt signaling pathway and cytokine receptor interaction pathways.