Genomic analysis of cefiderocol resistance in Klebsiella oxytoca
This BioProject comprises hybrid whole-genome sequencing of a clinical Klebsiella oxytoca isolate and two derived mutants exhibiting altered susceptibility to cefiderocol. A spontaneous cefiderocol-resistant mutant and a CRISPR-Cas9-engineered mutant carrying the mscM A3023T substitution were sequenced using Illumina MiSeq and Oxford Nanopore technologies. Hybrid assemblies were generated with Unicycler to investigate the genetic basis of cefiderocol resistance.