GLP-1 RAs or dietary fiber interventions study (PRJCA050830)
The GLP-1 receptor agonists or high dietary fiber interventions study is an open-label, multi-centered clinical trial in subjects with overweight or obese. FMT animal trial: The six-week-old germ-free male mice (C57BL/6J) were given oral gavage (3 times/week) with a 200-?l fecal slurry for 2 weeks. Mice were randomly divided into two groups: mW and mG groups (n=15 per group) inoculated with the fecal slurry of two donors who are from W (dietary fiber intervention) and G (semaglutide intervention) groups, respectively. Semaglutide and dietary fiber interventions animal trial: The eight-week-old SPF male mice (C57BL/6J) were fed western style diet (D12079B, SYSE Bio-tech. Co., Ltd., China) for 4 weeks to construct diet-induced obesity (DIO) model, then performed once every three days subcutaneous injection with semaglutide (0.1 mg/kg body weight, GWSD group) or semaglutide and oral gavage of dietary fiber (500 mg/kg bodyweight/day, WGWSD group) or normal saline as control (CWSD group) for 4 weeks. After the intervention, the mice of GWSD group and WGWSD group were fed normal chow diet (AIN-93G, SYSE Bio-tech. Co., Ltd., China) for 3 weeks as GNC group and WGNC group, respectively.