Lotus japonicus synthetic community family drop out
This project contains sequencing data generated from the Lotus japonicus synthetic community LjSC family dropout experiment. The dataset includes microbial inocula and plant-associated samples collected after exposure to modified versions of the L. japonicus synthetic community.Five reduced LjSC inocula were created by removing one bacterial family group at a time. The family groups removed were: Burkholderiaceae (LDT1), Caulobacteraceae (LDT2), Pseudomonadaceae (LDT3), Rhizobiaceae (LDT4) and a combined group representing the remaining families (LDT5). An additional treatment consisted of the complete LjSC inoculum (LDT6). A non-inoculated control was included (LDT7) consisting of L. japonicus plants grown in soil with 1N1P and without microbial addition.Plants were maintained for 21 days post-inoculation before sample collection. DNA was extracted from the Input inoculum (initial SynCom mixture), Roots, and Nodules. All treatments except LDT4 (no nodules) and LDT7 (only roots) include these sample categories.