Super-GBS sequencing of hybrid abalone (Haliotis discus hannai x H. fulgens) under Vibrio parahaemolyticus stress
Super-GBS sequencing was performed on hybrid abalone (Haliotis discus hannai x Haliotis fulgens) under Vibrio parahaemolyticus challenge. Genomic DNA was extracted from abalone individuals subjected to bacterial stress, including both mortality (72 h post-infection) and survival (10 days post-infection) groups. A Super-GBS library was constructed using restriction enzyme digestion and sequenced on an Illumina platform to identify genome-wide single nucleotide polymorphisms (SNPs) associated with stress response and survival.