Cover crops shape soil microbiome and ecological interactions in the soybean rhizosphere under long-term no-till
We analyzed a long-term field experiment (16 years), conducted during the 2021/2022 and 2022/2023 growing seasons, to evaluate soil fertility, enzymatic activity, and soybean yield, as well as the structure, diversity, composition, and networks of bacterial and fungal communities in a soybean-maize succession system. The study assessed the influence of different off-season cover crops, including a grass species (Ruzigrass), a legume (Sunn hemp), their combination (Mix), and a no-cover treatment (Fallow), using high-throughput sequencing techniques.