RNA-Seq Transcriptome Sequencing Data of THP-1 Cells Infected with the Rv3425 Knockout Strain (PRJCA048172)
To elucidate the mechanisms by which Rv3425 promotes the intracellular survival of M.tuberculosis, we utilized transcriptome to analyze the gene expression profiles of macrophages infected with M.tuberculosis. The RNA-seq results revealed a total of 1370 differential genes, with 685 genes being up-regulated and 685 genes being down-regulated (Fig. 4A). The top 50 differential genes indicated that genes related to immune responses, inflammation, and cell proliferation and differentiation were up-regulated, while genes related to neural functions were down-regulated. The results of Gene Ontology Biological Process enrichment analysis indicated that the differential genes (DEGs) of the knockout strain in infected macrophages were predominantly involved in immune-related functions, including promoting cytokine production, positive regulation of response to external stimulus, and cytokine-mediated signaling pathways. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEGs were involved in cytokine-cytokine receptor interactions and PI3K-Akt signaling pathway.