chrna5 regulates nicotine and ethanol self-administration and addiction-related behaviors in zebrafish (PRJCA049607)
chrna5 mutant zebrafish generated using CRISPR-Cas9 showed increased acute preference for nicotine and alcohol. Mutants had greater behavioral but reduced transcriptomic changes after drug exposure compared to wild type, indicating impaired homeostasis. They also showed altered appetite and circadian rhythms, suggesting links to comorbid disorders.