Analysis of the phyllosphere bacterial community of Solanum undatum in Xishuangbanna based on 16S rRNA sequencingbanna based on ITS high-throughput sequencing
Solanum undatum, belonging to the genus Solanum within the Solanaceae family and the Solanum wild species complex, is a crucial wild Solanum germplasm resource distributed in tropical regions of China, with important research value in the fields of stress resistance genetic improvement of Solanaceae crops, mining of characteristic secondary metabolites, and biodiversity conservation.The phyllosphere bacterial community of plants is the core biological factor for the host to respond to environmental stress and mediate the expression of disease and stress resistance traits. However, the phyllosphere microbial diversity and community characteristics of wild Solanum undatum populations distributed in the tropical Xishuangbanna region of China have not been systematically analyzed at present, and the lack of relevant research restricts the in-depth development and utilization of this wild germplasm resource. In this study, bacterial infection leaves of 13 wild Solanum undatum plants naturally growing in Xishuangbanna, Yunnan Province were collected, and the species composition, diversity characteristics, community structure and core functional groups of the phyllosphere bacterial community of this species were systematically analyzed by 16S rRNA gene high-throughput sequencing technology. The results indicate: Bacterial communities were dominated by Proteobacteria (59.4%) at phylum level; Alphaproteobacteria (32.5%) at class level; Rhodobacterales (18.6%) at order level; Rhodobacteraceae (16.2%) at family level; and an unclassified genus within Rhodobacteraceae (12.5%) at genus level.