Single-cell RNA sequencing of TDLNs in 4T1 mouse model treated with tumor resection or MWA (PRJCA044947)
Tumor draining lymph nodes (TDLNs) are central to antitumor immunity but their role in local tumor therapy is unclear. This study used scRNA seq to profile TDLNs from 4T1 mouse models on day 6 after surgical resection or microwave ablation (MWA). Single cell suspensions were processed with the GEXSCOPE platform and sequenced on Illumina NovaSeq 6000. The dataset enables analysis of immune cell heterogeneity and communication in TDLNs.