Transcriptional changes in lox9 mutants and WT plants under heat stress (PRJCA049348)
The wild type (WT) XiuShui 11 rice cultivar and lox9 mutant plants were used in this study. Seeds were surface-sown on Petri dishes containing distilled water and placed in a climate-controlled incubator at 26-28 degrees Celsius under a 16-hour photoperiod with LED illumination at an intensity of 100 micromoles per square meter per second. After germination, the seedlings were transferred to a hydroponic system and grown in an environmentally controlled chamber under a 13-hour light and 11-hour dark cycle with identical light intensity. When the seedlings reached 12 days of age, both the wild-type and mutant plants were exposed to a heat stress treatment at 42 degrees Celsius within the growth chamber. Tissue samples were systematically collected at multiple time points, specifically at 1 hour, 4 hours, 12 hours, and 24 hours after the initiation of the heat stress conditions.