Nudt21 knockdown induces transcriptomic reprogramming in mouse hepatocytes
A total of 6 samples were subjected to Nanopore Tail ISO-seq sequencing in this study, including 3 negative control groups (control-1 to control-3) and 3 Nudt21 knockdown groups (KD1 to KD3). Each sample produced approximately 5.5 million raw reads on average. After full-length identification and filtering, approximately 4.8 million high-quality full-length reads were obtained on average, with an alignment rate ranging from 87.64% to 96.34%. Principal component analysis (PCA) showed that the control groups and the knockdown groups were significantly separated along the PC1 axis, explaining 51.53% of the total variation.