HyperSTAMP: identifying targets of RNA binding proteins in Drosophila melanogaster with C-to-U edit
Surveying Targets by APOBEC-Mediated Profiling (STAMP) was one of the main RNA binding protein (RBP)-RNA modification enzymes (RME) methodologies which expressed fusing protein of RBP and wild type APOBEC1. Here we introduced H122L/D124N mutations to Rattus norvegicus APOBEC1 and named HyperSTAMP, which can monitor RBP-RNA interaction in Drosophila melanogaster. We utilized HyperSTAMP to reveal RNA targets of Heterogeneous nuclear ribonucleoprotein 48 (Hrp48), a part of ribonucleoprotein complex. HyperSTAMP filled in gaps for C-to-U marker in fly and provided possibilities for double edit experiment in RBP-RNA interaction research.