Transcriptomic Profiling of BV2 Cells with IRF5 Knockdown (PRJCA052545)
This study aims to elucidate the genome-wide role of the transcription factor IRF5 in regulating microglial gene expression and function within the context of amyotrophic lateral sclerosis (ALS). Microglia, the resident immune cells of the central nervous system, play critical yet complex roles in neuroinflammation and neurodegenerative diseases. To specifically investigate IRF5-dependent transcriptional programs under a disease-relevant stress, we performed RNA-seq on BV2 microglial cells transfected with the hSOD1-G93A mutant to model ALS, followed by siRNA-mediated knockdown of IRF5. By comparing the transcriptomes of IRF5-silenced cells to control ALS-model cells, we sought to identify key genes and signaling pathways downstream of IRF5 in this pathological setting.