Identification of naturally infected virus species in Solanum undatum from Xishuangbanna based on sRNA sequencing
Solanum undatum, a member of the Solanum wild species complex in the Solanaceae family, is a characteristic wild Solanum germplasm distributed in the tropical flora of Xishuangbanna, Yunnan, China, and a valuable gene donor for antiviral genetic improvement of Solanaceae crops. Virus disease is a major limiting disease in Solanaceae crop production. Identification of naturally infecting virus species in wild Solanum species is the key basis for dissecting host antiviral mechanisms and mining disease resistance resources, while there is still a gap in the virome research of Solanum undatum at present. In this study, 6 leaf samples of wild Solanum undatum plants with typical viral disease symptoms were collected from Xishuangbanna. Total RNA was extracted from the samples to construct a small RNA library, and single-end sequencing with 50 bp read length was performed on the HiSeq 2500 platform. The raw sequencing data were processed by ACGT101-miR software, and virus species identification was completed by alignment against the virus database. The results indicate: sRNA sequencing identified five viruses: TVCV (89.5%), PCV RNA 2 (68.1%), PVM (47.4%), WTMV (47.2%), and PVS (41.1%).