zebrafish single-cell monoallelic expression (PRJCA044959)
Monoallelic expression (MAE), the preferential transcription of one allele in diploid organisms, plays a crucial role in generating cellular heterogeneity and phenotypic diversity. While MAE has been extensively characterized in humans and mice, previous studies have been limited by clonal analyses or insufficient cell-type resolution, leaving its dynamics during development largely unexplored. In this study, we systematically investigate allele-specific expression in zebrafish by integrating whole-genome sequencing and single-cell RNA sequencing (scRNA-seq) across six individuals to characterize allele specific expression at single-cell resolution.