RNA-seq analysis of F-53B induced toxicity in human embryonic neural stem cells
This project investigates the developmental neurotoxicity of F-53B (6:2 Cl-PFESA) using a human embryonic neural stem cell (hNSC) model. Transcriptomic profiling (RNA-seq) was performed on hNSCs following a 24-hour exposure to graded concentrations of F-53B (0, 25, 50, 75, and 100 uM). The analysis identifies differentially expressed genes and enriched pathways involved in oxidative stress, mitochondrial dysfunction, and impaired neurogenesis. These data provide a mechanistic basis for quantitative Adverse Outcome Pathway (AOP) development and risk assessment of emerging per- and polyfluoroalkyl substances (PFAS).