Whole-genome sequencing of Rhodeus flaviventris for mitochondrial genome assembly
This project presents whole-genome sequencing data of the bitterling fish Rhodeus flaviventris (Cyprinidae: Rhodeus), obtained using the Illumina NovaSeq 6000 platform (150 bp paired-end reads). The primary objective of this sequencing is to assemble and characterize the mitochondrial genome of this species.Raw reads were quality-filtered, and high-quality reads were used for mitochondrial genome assembly. The assembled mitochondrial genome contains all 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. A small AT-rich gap is present in the ND3-ND4L intergenic region (the tRNA-Arg locus), which contains tandem repeats that are difficult to resolve using short-read sequencing. The redundant spurious sequences and duplicated tRNA-Arg annotations generated by assembly errors were manually corrected, with only the conserved single tRNA-Arg gene retained. The genomic data will support phylogenetic analyses, species identification, and population genetics studies of R. flaviventris and related Rhodeus species.Li, F., R. Arai and T.-Y. Liao 2020. Rhodeus flaviventris, a new bitterling (Teleostei: Cyprinidae: Acheilognathinae) from China. Zootaxa 4790 (no. 2): 329-340.