Human gut microbiota 16S rDNA sequencing following fecal filtration and centrifugation
This study systematically evaluates how different laboratory preparation methods affect the viability and taxonomic integrity of human fecal microbiota intended for fecal microbiota transplantation. Using 16S rDNA sequencing and LIVE/DEAD staining on stool samples, we compared filtration alone versus a range of centrifugation protocols. The findings provide evidence-based guidance for standardizing FMT preparation, aiming to maximize microbial survival and preserve community diversity while removing contaminants such as food debris and host cells.