Carbon source diversity shapes DOM chemodiversity and antibiotic resistome in earthworm casts
A validation experiment was conducted using a gut-derived Klebsiella pneumoniae strain to assess the effects of substrate diversity on antibiotic resistance features. Monoculture experiments were performed under different substrate diversity treatments. Whole-genome sequencing was applied to generate genome-resolved datasets for each treatment, enabling the analysis of treatment-associated variation in resistance-related genes. These data were used to validate the variation of ARGs in response to substrate diversity.