The Alteration of Subgingival Microbiome in Clear Aligner Therapy: A Pilot Study in Thai Patients
This study investigates the early-stage impact of Clear Aligner Treatment (CAT) on the subgingival microbiome in Thai patients. The primary objective is to characterize the taxonomic shifts and ecological dynamics associated with the mechanical presence of aligners using 16S rRNA sequencing. By comparing CAT users with a control group, this research aims to evaluate whether aligner therapy compromises subgingival homeostasis. The findings are expected to clarify the periodontal implications of CAT, providing foundational data to support evidence-based oral hygiene protocols and ensure long-term periodontal safety for patients undergoing clear aligner therapy.