PRJNA508748
T cell acute lymphoblastic leukemia evolves and thymus autonomy
Thymus autonomy is the capacity of the thymus to maintain T lymphocyte development and export independently of bone marrow contribution. Prolonging thymus autonomy was shown to be permissive to the development of T cell acute lymphoblastic leukemia (T-ALL), similar to the human disease. Here, performing thymus transplantation experiments in mice we report that thymus autonomy can occur in several experimental conditions, and all are permissive to T-ALL. We show that wild type thymi maintain their function of T lymphocyte production upon transplantation into recipients with several genotypes (and corresponding phenotypic differences), i.e. Rag2-/-?c-/-, ?c-/-, Rag2-/-IL-7r?-/-, and IL-7r?-/-. We found that the cellularity of the thymus grafts is influenced exclusively by the genotype of the host, i.e. IL-7r?-/- versus ?c-/-. Nonetheless, the difference in cellularity detected in thymus autonomy bore no impact on onset, incidence, immunophenotype or pathology of T-ALL. In all tested conditions, T-ALL reached an incidence of 80%, demonstrating that thymus autonomy bears a high risk of leukemia. We also analyzed the microbiota composition of the recipients and their genetic background but none of the differences found influenced the development of T-ALL. Taken together, our data support that interleukin 7 (IL-7) drives cellular turnover non-cell autonomously, which is required for prevention of T-ALL. We found no influence for T-ALL in the specific combination of the genotypic mutations tested (including the developmental block caused by Rag deficiency), in microbiota composition, or minor differences in the genetic background of the strains.