GSE119114
Chromatin-dependent cryptic promoters encode alternative  protein isoforms in yeast
Cryptic transcription is widespread and generates a heterogeneous group of RNA molecules of unknown function. To improve our understanding of cryptic transcription, we investigated their transcription start site usage, chromatin organization and post-transcriptional consequences in Saccharomyces cerevisiae. We show that  transcription start sites of chromatin-dependent internal cryptic transcripts resemble those of protein coding genes in terms of DNA sequence, directionality and chromatin accessibility. We define the 5' and 3' boundaries of cryptic transcripts and show that, contrary to RNA degradation-dependent ones , they often overlap with the end of the gene thereby using the canonical polyadenylation site and associate to polyribosomes. In fact, we show that chromatin-dependent cryptic transcripts can be recognized by ribosomes and may produce truncated polypeptides from downstream, in-frame start codons. Our work suggests that a significant fraction of chromatin-dependent internal cryptic promoters are in fact alternative truncated mRNA isoforms. The expression of these chromatin-dependent isoforms is conserved from yeast to human expanding the functional consequences of cryptic transcription and proteome complexity. Overall design: We identify 5' transcription start sites applying our 5'cap -sequencing approach as previously described (Pelechano et al. Nat Protocols. 2016  PMID: 26820793). To measue polyribosome association we applied the 5'cap -sequencing approach to either total RNA extracts or polyribosome-associated fraction after ultracentrifugation in sucrose gradient. We performed 2 biologically independent experiments.