GSE135864
Transcriptomic analyssis following EHMT1/2 inhibition
RNA was isolated from PEO1 olaparib resistant (PEO1-OR) cells grown in complete growth media or PEO1-OR clone 4 cells grown in complete growth media with 500 nanomolar of UNC0642 for 72 hrs. RNA-sequencing was performed to evaluate UNC0642-dependent transcriptional reprogramming, which contributes to resensitization of PEO1-OR to olaparib. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G). Overall design: PEO1-OR cells grown in complete growth media (four clones) compared to PEO1-OR clone 4 cells grown in complete growth media with 500 nanomolar UNC0642 for 72 hours (A1/A2 and B1/B2). PEO1-OR clone 4 cells with UNC0642 A1 and A2 were sequenced from a single RNA extraction thus these samples were merged into UNC_A.  PEO1-OR clone 4 cells with UNC0642 B1 and B2 were sequenced from a single RNA extraction thus these samples were merged into UNC_B. Please note that the four PEO1_Clone sample records (GSM4036839-GSM4036842) are duplicated sample records of the previous GSE117765 samples (GSM3308401-GSM3308404) for the convenient retrieval of the complete raw data from SRA.