GSE184109
Genome-wide maps of chromatin state of H3K79me2 in embryonic and neonatal cardiomyocytes of XMlc2-Cre;Dot1L mutants and controls
Epigenetic enzymes play critical roles in embryogenesis by defining higher-order chromatin structures required for the establishment of organ-specific transcriptional networks. In this study we investigated the role of the histone 3 lysine 79 methyltrasferase Dot1L in cardiomyocytes during development. We conducted chromatin immunoprecipitation experiments for H3K79me2 in embryonic (E16.5) and neonatal (P1) cardiomyocytes from Dot1L cardiomyocyte-specific conditional knock out and control mice. Genes that bear this modification in DOT1L controls lose it in cKOs, consistent with the model of DOT1L as the sole histone K79 methyltransferase. Overall, we identified enrichment of methylation of H3K79 in gene bodies and in regulatory regions associated wih trascription factors essential for cardiac patterning at E16.5 and with genes important for cardiomyocyte proliferation and maturation in the postnatal period. Overall design: H3K79me2 ChIP-seq profiling were performed on FACS purified cardiomyocytes from XMlc2-Cre;Dot1L mutant and controls at E16.5 and P1.