PRJNA276392
Drosophila melanogaster Targeted Locus (Loci)
We report the m6dA modification on the Drosophila genome. We collected ovary genomic DNA from 2-day wild-type and DMAD mutant files and performed DNA-immunoprecipitation(DNA-IP) experiments using anti-m6dA antibody, and the generated DNA library, which was subjected to a high-throughput sequencing analysis. In this assay, the IgG-immunoprecipited DNA from the same amount of wild-type ovaries was used as the control, and the high-throughput sequencing resulted in a range of approximately 4.2 to 5.5 million reads. In sum, we identified 161 and 491 peaks from wild-type and DMAD mutant samples. Importantly, m6dA is mainly utilized to modify the transposon sequence on the chromosomes. To link the m6dA modification with transposon expression, we employed wild-type and DMAD mutant samples to perform RNA-seq analysis.