GSE176148
H3K27me3 Cut&Tag profiling of bone marrow-derived alveolar macrophages from wildtype and Suz12 knockout mice
Macrophages are at the forefront of immune responses and their transcriptional programs are modified by their tissue environment and in response to immunological challenge. Post-translational modifications of histones, such as histone H3 lysine 27 tri-methylation (H3K27me3) by the polycomb repressive complex 2 (PRC2), are tightly associated with epigenetic regulation of gene expression. To explore whether H3K27me3 is involved in either the establishment or function of the mononuclear phagocyte system, we selectively deleted the SUZ12 gene in mice, a core component of PRC2. Consistent with the the role of PRC2 in maintaining transcriptional repression, we found that the majority of top-ranked genes enriched for H3K27me3 in wild type alveolar macrophages were upregulated in SUZ12 knock-out alveolar macrophages. Overall design: C57BL/6-Ly5.1 mice were lethally irradiated and reconstituted with either a ratio of 90% wt Ly5.2: 10% wt Ly5.1, or 90% Suz12 cKO Ly5.2: 10% wt ly5.1. Libraries were generated from 100,000 alveolar macrophages sorted from the lung of wt (2 biological replicates) or Suz12cKO mice (2 biological replicates). Samples were sequenced with an Illumina NextSeq platform, producing between 5-8 million paired-end 36bp reads per sample.