GSE168183
RNAseq analysis of BDAA treatment on YFV infected HEK293 cells
Purpose: to investigate the change in gene expression after BDAA treatment on YFV infected HEK293 cells. Methods: HEK293 cells were cultured; then cells were non-infected/infected with YFV, and then untreat/treat with BDAA. Total RNA were harvested and sent to RNA sequencing. Results: we demonstrated that BDAA treatment of YFV infected HEK293 cells at 18 hpi for 2 h increased expression of a total of 39 cellular genes, 33 of which are inflammatory cytokines, chemokines or ISGs, and the remaining are mostly genes related to NF?B, TNF-a and MAPK signal transduction. Conclusion: BDAA triggers an enhanced activation of RNA sensor-mediated inflammatory cytokine responses, most likely by YFV RNA. Overall design: There were four experimental groups: HEK293 cells were either uninfected or infected with YFV at MOI of 10 for 1 h. At 18 hours post infection, the cells were then either mock treated with DMSO or treated with 3µM of BDAA for 2 h. Total cellular RNA was extracted by TRIzol reagent, then sent to RNA sequencing.