GSE171467
Role of Cop1 on transcriptional output in 4T1 mouse triple negative breast cancer cells
Cop1 regulates stability of transcription factors. We determined the role of Cop1 in the regulation of Cebpd transciptional output. We generated 4T1breast cancer cells with CRISPR/Cas9 mediated Cop1 (gene symbol RFWD2) knockout. We treated control sgRosa26 and sgCop1 cells with vehicle or IFNgamma (20ng/ml) for 24 hours and isolated RNA for sequencing. Overall design: Examination of transcriptome in Cop1 intact and Cop1 loss 4T1 breast cancer cells in response to IFNgamma.