GSE131525
A composite immune signature parallels disease progression across T1D subjects (RNA-Seq Cohort 0 Cell Sorted)
Technical replicate testing was performed to determine the measurement precision of each analyte. Peripheral blood mononuculear cells were processed and frozen down from blood samples collected from  healthy control subjects (n=3) or subjects with Systemic Lupus Erythematosus (n=3). Three separate PBMC aliquots per subject were then independently thawed and sorted by flow into B cell, CD4+ T cell, CD8+ T cell, and monocyte populations.  Total RNA was isolated from the sorted cell populations and then RNAseq libraries were prepared. Overall design: The replicate testing cohort was used to determine the technical precision of each analyte by calculating the intra-subject coefficient of variation (CV) for three replicate aliquots (n=3) per blood draw from 6 subjects. For each analyte, the mean CV across subjects was then calculated.  Three independent PBMC aliquots per subject were thawed and sorted by flow cytometry into B cell, CD4+ and CD8+ T cell, and monocyte subsets before RNA sequencing.