GSE133273
Effects of transcription on genome - nuclear lamina interactions: RNA-seq data
In mammalian nuclei, transcriptionally active genomic regions tend to localize to the interior of the nucleus while inactive regions are often located at at the nuclear lamina.  In this study we activated specific genes in lamina-associated domains by TALE-VP64 or CRISPRa-mediated activation. We also reduced transcription of individual genes by knockout of promoter/enhancer regions, or by insertion of a transcription termination sequence. In each case we  generated  genome-wide DamID maps to determine changes in nuclear lamina interactions, and in selected cases we also generated Repli-seq maps and/or RNAseq data. Overall design: Transcription of individual genes was manipulated in human RPE-1 cells by  CRISPRa. Changes in gene expression were assayed by RNA-seq.