GSE173938
RNA sequencing of liver tissues from mice treated with or without ischemia
RIPK1 is a critical regulator of cell death and inflammation implicated in a multitude of human inflammatory and degenerative diseases. However, it remains elusive mechanistically whether and how RIPK1 may be activated under ischemia conditions.   Methods: Total RNA from the total livers of mice ( WT, Ripk1D138N, TNFR1/2 KO)  was purified using Qiagen RNeasy mini (Qiagen) according to the manufacturer's instructions. Library preparation and sequenceing analysis were performed by Quintara Biosciences.   Conclusions: Our study indicates that ischemia can induce RIPK1 kinase-dependent cell death and inflammation. Overall design: Total RNA was extracted from liver tissues from the following genotypes:  WT, Ripk1D138N, TNFR1/2 KO.  Each group has three repeats.