14295:257ee9eb-5c4b-4ffa-ab5f-38f8faf8e71d
Metabarcoding profiles of eukaryotic phytoplankton within coastal water off West Greenland and West Iceland
Water samples had been taken at 25 stations during MSM21-3 cruise at depths of about 10 m with a rosette sampler at the coasts of West Greenland (Uummannaq Fjord, Vaigat Strait, Disko Bay) and West Iceland (Arnafjördur, Breidafjördur, Faxaflóe) between 2012-07-27 and 2012-08-08. At 16 stations also deeper water samples had been taken at depths between 40m and 625m. All samples were size fractionated to enrich Micro- (20-50 µm), Nano- (3-20 µm) and Picoplankton (0.2-3 µm). Of selected fractions DNA was extracted and the rDNA of the D1/D2 region of the 28S rDNA (primer D1R-F and D2C-R) was amplified and resulting amplicons were sequenced on a 454 GS FLX sequencer. In total 80 metabarcoding samples were obtained.