GSE125440
Loss of the nucleolar DUB USP36 impairs Parkin dependent mitophagy via downregulation of autophagic components
The Parkin dependent final elimination of defective mitochondria is halted by loss of the nucleolar DUB USP36. The authors suggest a general failure in autophagy induction due to a decline in Beclin-1 and ATG14 levels. Reduction of the epigenetic mark histone 2B lysine 120 ubiquitination possibly contributes to the observed phenotype. Overall design: comparison of mRNA expression levels from control siRNA and USP36 siRNA transient transfected HeLa cells of five independent experiments