PRJNA542992
Intestinal microbiota of grass carp
320 grass carp (Ctenopharyngodon idellus; initial weight 8.18 ± 0.81 g) were fed four diets for eight weeks. Two were practical diets: FM diet , containing 10% fishmeal without rapeseed meal, RM diet containing 50% rapeseed meal without fishmeal; the other two were semi-purified diets without (T0) or with 1.25% (T1) of supplemental hydrolysable tannin respectively. The approximate content of tannin in the RM diet was 1.31%, which was roughly close to T1, and FM diet close to T0. Following the feeding period, three fish were removed from each cage (12 fish/ group, collected 5-6 h after the last feeding), anesthetized and killed as described above. The entire intestine tract was removed aseptically and digesta samples (allochthonous bacteria) were collected by carefully scraping with a sterile spatula into sterile containers and storage at -80 °C prior to DNA extraction.