PRJNA453758
Optimizing exosomal RNA isolation for RNA-Seq  analyses of archival sera specimens
RNA-Seq libraries were prepared with exosomal RNAs extracted from human cell-free serum, cataloguing both coding and non-coding exosomal transcripts. This method provides researchers with strategic options to prepare RNA-Seq libraries and compare RNA-Seq data quantitatively from minimal volumes of fresh and archival human cell-free serum for disease biomarker discovery.