Gametocytogenesis_In_Plasmodium_Berghei-sc-6317
Gametocytogenesis_In_Plasmodium_Berghei
This study contains two scRNA-seq datasets that were generated using Smart-seq2 and 10x Genomics 3' respectively. The Smart-seq2 dataset contains knockout mutant P. berghei parasites and matched wild-type controls. Knockout genes were selected using the results from a genome-scale reverse genetic screen  to identify candidate genes involved in sex determination and/or sex ratio decisions. The 10x Genomics 3' v2 chemistry dataset contains wild-type P. berghei single cells that cover all blood-stage cell types, including developing gametocytes. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Deciphering how malaria parasites switch from asexual proliferation to sexual development is of fundamental importance to eradicating malaria transmission. The initial commitment to sexual development is only partly understood, and how sex is determined has remained a complete mystery. To understand these events, we used the results from a parallel genome-scale reverse genetic screen covering hundreds of genes in rodent malaria parasites, to identify eleven uncharacterised candidate genes involved in sex determination and/or sex ratio decisions. We then applied Smart-seq2 and 10x Genomics 3' scRNA-seq to determine how the transcriptional landscape within these knockout mutant Plasmodium parasites varies, compared to wild-type parasites.