Mouse_melanoma_cell_lines_transcriptome_sequencing-sc-2012-08-09T13:34:12Z-2326
Mouse_melanoma_cell_lines_transcriptome_sequencing
n this project we have sequenced the transcriptome of several comonly used mouse melanoma cell lines. Our aim is to understand what mutations and rearrangements are found in these cell lines to gain a better understanding of the genes that contribute to melanomagenesis. As these cell lines are used as models of melanoma these sequences represent an important resource for functional studies.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
In this project we have sequenced transcriptome of several comonly used mouse melanoma cell lines. Our aim is to understand what mutations and rearrangements are found in these cell lines to gain a better understanding of the genes that contribute to melanomagenesis. As these cell lines are used as models of melanoma these sequences represent an important resource for functional studies. As part of this project we have generated transcriptomes from 5 replicate cultures of each cell line. Our plan is to catologue the variants found in each line and to assess their importance using knockodown and overexpression approaches