PRJNA558668
Global profile of miRNA in Rat HuR silencing intestinal epithelial cells
The IEC-6 cell line, derived from normal rat intestinal crypt cells were maintained in DMEM supplemented with 5% heat-inactivated FBS. The siRNA targeting HuR (siHuR) and non-targeting negative control siRNA (si-NC) were purchased from Santa Cruz Biotechnology. IEC-6 cells grown to 50% confluence were transfected with 20 nmol/L siHuR or si-NC using Lipofectamine3000 transfection reagent. At 72 h post-transfection, cells were harvested for the experiment. The small RNA sequencing was performed by BGI International (Wuhan, China) using the BGISEQ-500 sequencing system. Clean tags were mapped various sRNA databases, including miRBase, Rfam, siRNA, piRNA, snoRNA, and others. Based on the read number that mapped to the genome, the small RNA expression level was calculated as Transcripts Per Kilobase Million.