E-MTAB-8199
scRNA-seq of CD45+ cells taken from the ear epidermis from K14E7 transgenic and wildype C57/BL6 mice
Langerhans cells (LC) in skin help initiate the immune response to locally presented antigens. We performed high-resolution  single-cell RNA-sequencing (scRNAseq) analysis for antigen presenting cells including LC in normal mouse skin, and in mouse skin expressing the  human papillomavirus (HPV) 16 E7 oncogene. Ear skin was collected from normal and trangenic mice. Dissociated CD45+ cells were processed for scRNA-seq using the 10X Genomics Chromium 3' gene expression kit (v2).
Langerhans cells (LC) in skin help initiate the immune response to locally presented antigens. We performed high-resolution  single-cell RNA-sequencing (scRNAseq) analysis for antigen presenting cells including LC in normal mouse skin, and in mouse skin expressing the  human papillomavirus (HPV) 16 E7 oncogene. Ear skin was collected from normal and trangenic mice. Dissociated CD45+ cells were processed for scRNA-seq using the 10X Genomics Chromium 3' gene expression kit (v2).