PRJNA416145
paper pulp sludge enviromental metagenome
This Bioproject describes sampling and consequent metagenomic analysis of the microbial community inhabiting primary wastewater sludge of a coastal BC paper mill. The metagenomic DNA was used for creating a fosmid library that was mined for carbohydrate active enzymes (cAZymes) using a high throughput functional screen and selected clones applied for consolidated bioprocess development. In-silico analysis of the metagenomic data using several bioinformatics pipelines, most importantly Metapathways, was also conducted to complement experimental findings. The metagenome was taxonomically binned and functionally compared to data from other biomass waste environments. This project establishes proof of concept of application of metagenomics to circular bioeconomy development.