PRJNA803387
ESBL-producing Enterobacteria isolated from gut microbiota of hospitalized patients and pOXA-48 transconjugants
This BioProject includes the following strain collections: (i) PF collection: 50 representative ESBL-producing enterobacteria wild type clones isolates selected from the R-GNOSIS collection. This collection was constructed in the Ramon y Cajal University Hospital as part of an active surveillance-screening programme for detecting patients colonised by ESBL/carbapenemase-producing enterobacteria, from March 4th, 2014 to March 31st, 2016 (R-GNOSIS-FP7-HEALTH-F3-2011-282512, https://cordis.europa.eu/project/id/282512/reporting/es, approved by the Ramon y Cajal University Hospital Ethics Committee, Reference 251/13). Samples were isolated and characterized as described in Hernandez-Garcia, et al. 2018 (10.1093/jac/dky284). These samples include 25 Escherichia coli and 25 Klebsiella spp. (20 K. pneumoniae, 4 K. quasipneumoniae and 1 K. variicola) wild type isolates representative of these species diversity in the R-GNOSIS collection. They are not carbapenem-resistant. (ii) TC collection: 45 pOXA-48 transconjugants derived from the PF collection (21 E. coli, 19 K. pneumoniae 4 K. quasipneumoniae and 1 K. variicola). Transconjugants were obtained following the protocol described in Alonso-del Valle et al. 2021 (https://doi.org/10.1038/s41467-021-22849-y).