ena-STUDY-NATIONAL HEART AND LUNG INSTITUTE, IMPERIAL COLLEGE LONDON-10-07-2019-10:06:13:451-1399
Characterizing the Fungal Airway Microbiome in Cystic Fibrosis and Bronchiectasis by Next Generation Sequencing
The prevalence of fungal airways disease is increasing yet the lack of standardised diagnostic criteria make interpretation of culture results challenging. Development of effective management strategies requires a more accurate and comprehensive understanding of the fungal airway microbiome.Through the use of next generation sequencing of the ITS2 region we aimed to assess the load and diversity of fungi in patients with chronic bronchial infection. Sputum samples from 87 adults with cystic fibrosis and 24 with bronchiectasis were used to investigate patterns in fungal community composition between those with recognized fungal disease and disease controls. Only 49% of the samples were positive by fungal culture while all samples produced high quality fungal sequencing profiles. Patients with Cystic fibrosis were found to have a higher fungal biomass with lower fungal diversity than those with bronchiectasis. Candida was a common OTU is all individuals, however, Candida parapsilosis was found only in cystic fibrosis sputum while Candida albicans was most common in those with bronchiectasis. This study provides a more accurate framework to characterize the fungal airways disease-phenotypes in adult suppurative lung diseases.
The prevalence of fungal airways disease is increasing yet the lack of standardised diagnostic criteria make interpretation of culture results challenging. Development of effective management strategies requires a more accurate and comprehensive understanding of the fungal airway microbiome.Through the use of next generation sequencing of the ITS2 region we aimed to assess the load and diversity of fungi in patients with chronic bronchial infection. Sputum samples from 87 adults with cystic fibrosis and 24 with bronchiectasis were used to investigate patterns in fungal community composition between those with recognized fungal disease and disease controls. Only 49% of the samples were positive by fungal culture while all samples produced high quality fungal sequencing profiles. Patients with Cystic fibrosis were found to have a higher fungal biomass with lower fungal diversity than those with bronchiectasis. Candida was a common OTU is all individuals, however, Candida parapsilosis was found only in cystic fibrosis sputum while Candida albicans was most common in those with bronchiectasis. This study provides a more accurate framework to characterize the fungal airways disease-phenotypes in adult suppurative lung diseases.