GSE127846
FMRP cTag CLIP from mouse CA1 pyramidal neurons
Loss of the neuronal RNA binding protein FMRP causes Fragile X Syndrome (FXS), the most common cause of inherited intellectual disability, yet it is unknown which brain regions and cell types within them contribute to disease pathophysiology. We used conditional tagging of FMRP and CLIP (cTag FMRP CLIP) to examine FMRP targets specifically in CA1 hippocampal neurons, a critical cell type for learning and memory known to have altered synaptic function in FXS. Integrating this data with analysis of ribosome-bound transcripts from the same neuronal population revealed CA1-enriched binding of autism-relevant mRNAs, and unexpected CA1-specific regulation of transcripts encoding circadian proteins. Overall design: 3 biological replicates of FMRP cTag CLIP in mouse CA1 pyramidal neurons. Each replicate includes a sample prepared from Fmr1-cTag/Camk2a-Cre hippocampal tissue and a sample prepared from animals without Cre expression as a negative control.