GSE112069
Function of Epithelium BRG1 in colitis and CAC
Here we show that epithelial BRG1, the catalytic subunit of SWI/SNF complex is critical for colitis and colitis-associated-cancer. Depletion of BRG1 in colonrectal epithelial cells make mice develop Spontaneous colitis. To dissect underlying mechanism, we conducted gene expression profile analysis (RNA-Seq) by using primary colonrectal epithelial cells isolated from BRG1CTRL and BRG1IEC-KO(Tamoxifen induced BRG1 knock-out) mice to gain molecular insights into the affected biological processes. To this end, colorectal epithelial cells were isolated after 14 days of tamoxifen treatment, on which day BRG1-depleted mice showed little morphological defects in colon in comparison with control littermates. IECs were isolated by EDTA isolation buffer. Each sample contains pooled mRNA from 3 mice, and was subjected to Hiseq RNA-Seq, performed by BGI Tech Solutions Co., Ltd. Overall design: Genome-wide expression profiling of primary colon epethelial cells isolated form BRG1CTRL and BRG1IEC-KO(Tamoxifen induced BRG1 knock-out) mice,2 weeks prior to cell collection BRG1F/F and BRG1ERT2-F/F mine were treated with tamoxifen.