GSE107519
Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2 II
Previous studies suggested that MeCP2 binds to linker DNA and competes with linker histone H1 to regulate chromatin structure, but this hypothesis has never been tested in vivo. Here, we expressed Flag-tagged H1.0 in forebrain excitatory neurons in mice and performed ChIP-Seq to reveal H1.0 distribution and its relationship with MeCP2. Unexpectedly, we detected no major change in H1.0 upon MeCP2 depletion, revealing that MeCP2 functions independent of linker H1.0. Overall design: ChIP-seq of MeCP2 from the mouse frontal cortices, which were obtained from the wild-type (WT) and Cam-tTA:Fl-H1.0 mice expressing Flag-tagged H1.0 in the Camk2a+ excitatory cells (three biological replicates). MeCP2 ChIP fh-Mecp2+/y peaks are included in the file Mecp2.FH.MACS_peaks.bed available on the SuperSeries record.