PRJNA841051
Bulk RNA-seq in NF2 Wildtype and knockout H1-hESCs across cardiomyocyte differentiation timepoints
To investigate the role of NF2 during cardiomyocyte cell-fate specification, we harvested RNA lysates across key cardiac differentiation timepoints from wild-type and NF2 mutant H1-hESCs, to profile gene expression changes across cardiomyocyte differentiation. By performing differential gene expression analysis across time-series RNA-seq data, our analyses unveiled global loss of cardiogenic identity in NF2 mutant cells, and radical shift towards epithelial cell-fate. Here we constructed differentiation trajectory using Trade-seq and we revealed an important role for NF2 in removing early regulatory roadblocks as cells transit from pluripotency to cardiomyocyte-lineage. These datasets detects the transitioning phase from pluripotent stem cells to mesendoderm to terminally differentiated cardiomyocytes by Day 14. Total RNA library was prepared using illumina TruSeq Stranded Total RNA Library Prep HMR kit (illumina, Cat#: 20020596) and sequenced in paired-end reads using HiSeq4000 (Total 41 Samples).