PRJNA491730
Prunella vulgaris nanopore root transcriptome
Total RNA was extracted from roots of Prunella vulgaris using a CTAB based method. mRNA was selected from 16 µg total RNA using Dynabeads Oligo (dT)25 (ThermoFisher Scientific) yielding 119 ng mRNA. The mRNA was converted to cDNA using the Oxford Nanopore cDNA-PCR Sequencing Kit (SQK-PCS108), and sequenced on the GridIon.