E-MTAB-5252
RNA-Seq of Drosophlia adult mid-gut with the induction of RNAi against the largest subunit of RNA polymerase III.
TIGS>dC160RNAi females were fed RU486 from day 2 of adulthood to induce the RNAi expression, 10 guts were dissected per sample (the dissections were batched), RNA extracted with TRIZOL, cleaned up with Quiagen RNEasy with on-column DNase treatment, rRNA depleted (Ribo Zero Gold) and sequenced on the Illumina platform
TIGS>dC160RNAi females were fed RU486 from day 2 of adulthood to induce the RNAi expression, 10 guts were dissected per sample (the dissections were batched), RNA extracted with TRIZOL, cleaned up with Quiagen RNEasy with on-column DNase treatment, rRNA depleted (Ribo Zero Gold) and sequenced on the Illumina platform