GSE134172
RNAseq of mouse colon epithelial cell fraction in Lingo3, and WT mice
The purpose of this experiment is to deep sequence transcripts in WT mice and mice lacking Lingo3, and compare gene expression in the epithelial cells of the colon between wild type and mutant mice. Colon tissue was isolated from each mouse, and epithelial cells were removed from the colon by washing the tissue with 5 mM EDTA + 2.5 mM DTT solution. Overall design: wild type and Lingo3 mutant mice, 3 replicates for each condition, 6 samples total