GSE194254
ScRNAseq analysis of EC sensitized mouse skin
We performed single-cell RNA-seq to transcriptionally characterize the populations and gene expression in the skin of mice epicutaneously sensitized Overall design: Balb/c mice were epicutaneously sensitized for 10 days. 2 days later the mouse skin was collected and enzymatically digested to generate a sjingle cell suspension. Barcoding and library preparation were done using  the 10x Genomics Chromium platform, with the Chromium Single Cell 3' Library & Gel Bead Kit v2 and Chromium Single Cell 3' Chip kit v2 according to the manufacturer's instructions.