GSE174324
Single-cell transcriptome sequencing of macrophages in acute kidney injury
We uesd single-cell transcriptome sequencing technology to sequence the mononuclear phagocytes in the mice kidney, blood and spleen after acute kidney injury, and comprehensively describe characteristics of mononuclear phagocytes. Overall design: We used a unilateral ischemia-reperfusion renal injury (uIRI) 45minAKI model. The mouse kidney, spleen, and peripheral blood were taken 1 and 3 days after surgery, and the kidney, spleen and peripheral blood of normal mice were used as controls.  Mononuclear phagocytic cells were sorted by  FACS  and then performed single cell  transcriptome sequencing. 6 mice at each time point.