ena-STUDY-UNIVERSITY HOHENHEIM-20-05-2015-07:31:39:251-97
Evaluation of commercial DNA extraction kits for analysing microbial diversity in the pig gastrointestinal tract
15 manual and four automated procedures of commercial DNA extraction kits were evaluated for their efficiency to extract DNA from pig feces and ileal digesta samples. Illumina amplicon sequencing, spanning V1-2 and V5-6 regions of 16S rRNA gene,  was used to characterise the diversity and composition of the microbial community.
15 manual and four automated procedures of commercial DNA extraction kits were evaluated for their efficiency to extract DNA from pig feces and ileal digesta samples. Illumina amplicon sequencing, spanning V1-2 and V5-6 regions of 16S rRNA gene,  was used to characterise the diversity and composition of the microbial community.