GSE65105
Effect of 4hr cortistatin A treatment on gene expression in SET2 cells.
We characterized the marine natural product cortistatin A (CA) as an inhibitor of CDK8 to determine whether pharmacologic inhibition of CDK8 regulates super-enhancer function and inhibits AML proliferation. In this series, we examine the transcriptional effect on SET2 cells of 4hrs exposure to CA to identify immediate effects. Overall design: SET2 cells were treated in triplicate with either DMSO or CA for 4hrs after which RNA was harvested and prepared for RNA sequencing to assess transcriptional changes.