ena-STUDY-ERA7-30-09-2014-16:16:34:385-776
Characterization of Ehrlichia mineirensis genome sequence: insights into Ehrlichia evolution.
Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. Ehrlichia mineirensis is a new Ehrlichia species isolated from Rhipicephalus (Boophilus) microplus ticks in Brazil that is closely related to E. canis. The phylogenetic analysis based on 16S rRNA , groEL, dsb  and gltA  showed that E. mineirensis falls in a clade separated from any previously reported Ehrlichia  spp. The molecular analysis of the ortholog of gp36,  the major immunoreactive glycoproteins in E. canis  and ortholog of the E. chaffeensis gp47,showed a unique tandem repeat of 9 amino acids (VPAASGDAQ) when compared with those reported for E. canis , E. chaffeensis  and the related mucin-like protein in E. ruminantium.   Genome sequence yielded a genome assembly of 1414910 bp and 187 contigs with a N50 of 32220. The assembly showed high overall similarity with the E. canis Jake genome while harboring many indels and substitutions sparse along the sequence.
Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. Ehrlichia mineirensis is a new Ehrlichia species isolated from Rhipicephalus (Boophilus) microplus ticks in Brazil that is closely related to E. canis. The phylogenetic analysis based on 16S rRNA , groEL, dsb  and gltA  showed that E. mineirensis falls in a clade separated from any previously reported Ehrlichia  spp. The molecular analysis of the ortholog of gp36,  the major immunoreactive glycoproteins in E. canis  and ortholog of the E. chaffeensis gp47,showed a unique tandem repeat of 9 amino acids (VPAASGDAQ) when compared with those reported for E. canis , E. chaffeensis  and the related mucin-like protein in E. ruminantium.   Genome sequence yielded a genome assembly of 1414910 bp and 187 contigs with a N50 of 32220. The assembly showed high overall similarity with the E. canis Jake genome while harboring many indels and substitutions sparse along the sequence.