E-MTAB-9992
A long-term feeder-free culture method for human pancreatic progenitors on extra cellular matrix or matrix-free polymers
The dataset comprises RNA-seq information of human embryonic stem cell derived pancreatic progenitors and their proliferating cells cultured in defined condition. Total RNA of each sample was isolated, labelled, and purified by standard chemistry. Sequencing libraries were prepared from 250ng of purified total mRNA using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Single-read RNA-sequencing was performed using the NextSeq 500 (Illumina) with 75 High Output Kit (Illumina, FC-404-2005).
The dataset comprises RNA-seq information of human embryonic stem cell derived pancreatic progenitors and their proliferating cells cultured in defined condition. Total RNA of each sample was isolated, labelled, and purified by standard chemistry. Sequencing libraries were prepared from 250ng of purified total mRNA using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Single-read RNA-sequencing was performed using the NextSeq 500 (Illumina) with 75 High Output Kit (Illumina, FC-404-2005).