ena-STUDY-UNIVERSIDAD DE OVIEDO-02-05-2018-18:24:52:008-42
adpAa pleiotropic regulator S. argillaceus
AdpA (A-factor-dependent protein A) is a key pleiotropic regulator first discovered in S. griseus where it acts as a positive transcriptional regulator of cell cycle differentiation and antibiotic production. In the course of our studies on regulation of the mithramycin gene cluster in S. argillaceus, we found that inactivation of an adpA ortologue gene blocked mithramycin production. Noticeable, further analysis of those cultures extracted with different solvents revealed that this mutant strain accumulated two compounds with similar retention times, which were not detected in the wild type strain.
AdpA (A-factor-dependent protein A) is a key pleiotropic regulator first discovered in S. griseus where it acts as a positive transcriptional regulator of cell cycle differentiation and antibiotic production. In the course of our studies on regulation of the mithramycin gene cluster in S. argillaceus, we found that inactivation of an adpA ortologue gene blocked mithramycin production. Noticeable, further analysis of those cultures extracted with different solvents revealed that this mutant strain accumulated two compounds with similar retention times, which were not detected in the wild type strain.