PRJNA738960
RNA  sequencing
Next-generation sequencing(NGS) has revolutionized systems-based analysis of celluar pathways. The goals of this study are to compare NGS-derived heart tissues of Stat5flox/flox and Stat5-cKO mice with or without electroacupuncture followed by myocardial I/R injury transcriptome profilling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT-PCR) methods and to evaluate protocols for optimal high-throughput data analysis