GSE135138
Preliminary RNA-seq data obtained from H1975 and H661 cells exposed to AAT protein
Although the classical function of AAT is to inhibit neutrophil proteases, there is evidence that the role of AAT in inflammation is multifaceted. AAT possesses anti-inflammatory activity against immune cells, affects leukotriene B4, TNF-a, IL-8, IFN-? and IL-1ß production and expresses potent anti-apoptotic properties. In addition, AAT has been shown to inhibit the activity of natural killer cells against tumor cells, which indicates that AAT may play multiple functions during cancer development. Therefore, in a situation of occurring cancer cells higher AAT levels may favor microenvironment towards malignant progression. High expression of SERPINA1 gene encoding acute phase protein, a1-antitrypsin (AAT), is associated with poor prognosis in various tumors. Here we examined the significance of SERPINA1 and AAT protein in non-small-cell lung cancer (NSCLC) patients and NSCLC cell lines. Overall design: The H1975 and H661 cells were grown in serum free medium without or with addition of 2 mg/ml AAT for 24 hours. Afterwards, the total RNA was isolated and quality was assessed using 1 % agarose gels as well as by Agilent 2100 Bioanalyzer using Agilent RNA 6000 Nano Kit. Libraries were prepared in duplicates by using 200 ng of each cell line.