Globodera pallida genomic sequencing-sc-2010-08-18
Globodera pallida genomic sequencing
The aim of the project is to produce a reference genome for Globodera pallida at the level of an improved draft.  A mixture of sequencing technologies will be employed including capillary, Illumina and 454 sequencing.  To reduce polymorphism we will be using a combination of mixed libraries of large numbers of worms, as well as whole genome amplified libraries from smaller numbers.  The Potato Cyst Nematodes (PCN), Globodera pallida and Globodera rostochiensis are the most economically important nematode problems of the U.K. potato industry.  Breeding for resistance since 1950s has produced a few commercially acceptable varieties with resistance to G. pallida. Effective control of G. pallida is an essential requirement to maintain the competitiveness of U.K. potato production. The aim of this project is to obtain the full genome sequence of G. pallida (standard Pa2/3 pathotype, isolate Lindley) to aide in defining new control measures and pest management strategies.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Sequencing different worm populations of Globodera pallida for assembly and analysis.