ena-STUDY-UNIVERSITY OF BRITISH COLUMBIA-08-10-2017-00:15:11:663-60
16S rRNA gene sequencing (Primers 518F and 806R, Illumina MiSeq 150pb-PE) of SIP fractions following glucose amendment in Organic vs Conventional management systems and annual vs perennial crops.
Long-term contrasts in agricultural management can shift soil resource availability with potential consequences to microbial carbon (C) use efficiency (CUE) and the fate of C in soils. Isothermal microcalorimetry was combined with 13C-labelled glucose stable isotope probing (SIP) of 16S rRNA genes to test the hypothesis that organically managed soils would support microbial communities with greater CUE compared to conventional soils due to a legacy of lower resource availability and a resultant shift towards communities supportive of more oligotrophic taxa.
Long-term contrasts in agricultural management can shift soil resource availability with potential consequences to microbial carbon (C) use efficiency (CUE) and the fate of C in soils. Isothermal microcalorimetry was combined with 13C-labelled glucose stable isotope probing (SIP) of 16S rRNA genes to test the hypothesis that organically managed soils would support microbial communities with greater CUE compared to conventional soils due to a legacy of lower resource availability and a resultant shift towards communities supportive of more oligotrophic taxa.