GSE174315
Comparative profiling of microRNA transcriptome in different developmental phases  and analysis of microRNAs associated with reproductive development in the Brown Planthopper, Nilaparvata lugens
In the present study, small RNA libraries derived from three developmental phases (1st day after eclosion, 3rd day after eclosion and 5th day after eclosion) of short-winged morphs female adults were constructed and sequenced. A total of 15,571,298, 16,362,568 and 15,147,276 raw reads were obtained from three BPH small RNA libraries, respectively. Moreover, we identified 905 miRNAs, including 263 conserved (belonging to 37 families) and 642 novel miRNAs. In addition, the expression profiles of these miRNAs were assessed across different developmental stages Specifically, 22, 19, and 12 miRNAs were differentially expressed between3d and 1d, 5d and 1d, and 5d and 3d, respectively. A total of 43 miRNAs were differentially expressed in the three developmental phases, and 7 miRNAs were validated using quantitative RT-PCR. Target Scan and miRanda predicted14568 putative targets for 43 differentially expressed miRNAs within the 3'UTRs sequences of BPH transcriptome. Moreover, GO and KEGG pathway analysis of the predicted miRNA targets further illustrate the likely roles for these differentially expressed miRNAs in reproduction. Overall design: Small RNA libraires from three developmental stages (1st day after eclosion, 3rd day after eclosion and 5th day after eclosion)