ena-STUDY-VIRGINIA TECH-22-08-2017-00:10:28:226-320
Hot water plumbing microbiome
Heat shock is a potential control strategy for Legionella pneumophila in hot water plumbing systems.  However, its effectiveness, especially its influence on the broader plumbing microbiome remains largely unknown.  This study compares the pre- and post- heat shock microbiota via the application of a lab-scale recirculating hot water plumbing rig.  A second parallel rig was employed to simulate a thermal disruption scenario.  To probe the microbial composition, 16S rRNA gene amplicon sequencing was applied on Illumina MiSeq platform (2 * 300 cycles), targeting V4 region (barcoded 515F/ 926R).
Heat shock is a potential control strategy for Legionella pneumophila in hot water plumbing systems.  However, its effectiveness, especially its influence on the broader plumbing microbiome remains largely unknown.  This study compares the pre- and post- heat shock microbiota via the application of a lab-scale recirculating hot water plumbing rig.  A second parallel rig was employed to simulate a thermal disruption scenario.  To probe the microbial composition, 16S rRNA gene amplicon sequencing was applied on Illumina MiSeq platform (2 * 300 cycles), targeting V4 region (barcoded 515F/ 926R).