GSE192808
RNA-Seq of CTRL and PGR overexpressing MCF7 intraductal xenografts
Using a large variety of methods we demostrate that PGR can be a driver of tumor proliferation even in absence of extensive E2. In this specific experiment we overexpress PGR in MCF7 cells intraductally xenografted into the ductal tree of a mouse mammary gland, which provides post-menopausal levels of E2. Despite these conditions the cells thrive and with the PGR overexpression the growth rate is double the control cells. PGR overexpressing cells exhibit consistently more invasive and metastatic properties. Overall design: MCF7 CTRL and PGR overexpressing cells were grown intraductally for 45 days. The mammary glands collected at endpoint were snap-frozen, RNA extracted and sequenced.