GSE161650
PolyA-sequencing in Jurkat cells upon CDK12 depletion.
We analyzed the 3' end of polyadenylated transcripts in Jurkat cells treated with either THZ531 (vs. DMSO) or the CDK12 degrader BSJ-4-116 (vs. BSJ-4-116NC). Overall design: Expression of polyA transcripts in Jurkat cells treated with 250 nM THZ531 (vs. DMSO control) or 50 nM BSJ-4-116 (vs. BSJ-4-116NC) for 8h using the RNA-seq library kit (QuantSeq 3' mRNA Sequencing REV, Lexogen) in triplicate, for a total of 12 individual samples.