GSE160134
Transcriptomic analysis of young roots of spruce tianshan treated with 1mMDHAP (3,4 dihydroxyacetophenone)
In past studies the hypothesis of "self-toxicity of natural regeneration barriers of Spruce tianshan" were proposed, and verified that 3, 4-dihydroxyacetophenone (DHAP) contained in litter of Spruce Tianshan was the direct cause of population regeneration barriers.The molecular basis of DHAP-induced autotoxicity was explored through transcriptomic analysis of young roots of spruce tianshan treated with DHAP (3,4 dihydroxyacetophenone). Overall design: The overall experiment included the construction of four libraries: 19E15R-79, 19E15R-80, 19E15R-81, 19E15R-82;12 samples and biological replicates were analyzed: (1) Water treated roots: W_root-1, W_root-2, W_root-3;(2) Suppressed seedling roots were grown with high concentrations of DHAP: HD_Iroot-1, HD_Iroot-2, HD_Iroot -3 (3) Roots of high seedlings treated with high concentrations of DHAP: HD_Hroot-1 HD_Hroot-2 HD_Hroot-3 (4) Roots of dwarf seedlings treated with high concentration DHAP: HD_Sroot-1 HD_Sroot-2 HD_Sroot-3