GSE182759
Gene expression and intron retention analysis of 15.5K-knockdown human cells
15.5K is a RNA binding protein involved in the Minor Spliceosome. Here, we used shRNAs to knock down 15.5K in HEK293T cells to analysis the function of 15.5K in gene expression and intron retention. Overall design: We analysed RNA from negative control HEK293T (shNC) cells and two 15.5K-knockdown  (sh15.5K-1 and sh15.5K-2) cells. For each condition, mRNA-seq analysis was conducted in triplicate from three independent expriments. We compare 15.5K-knockdown cells vs WT cells.