ena-STUDY-INRA UMR 1319-30-08-2018-15:30:22:066-350
Yarrowia lipolytica H222 draft genome
The genome of Yarrowia lipolytica strain H222 has been sequenced using Illumina Solexa HiSeq2000 technology with a paired-end library (275X, average insert of 400 bp.) and a mate-pair library (47X, average insert of 8 kb.). The raw reads were trimmed with Trimmomatic and cutadapt. The assembly was done using SOAPdenovo2 with a kmer of 77.The current draft genome sequence is composed of 17 scaffolds larger than 5 kb (all nuclear), for a total size of the nuclear DNA of 20,519,037 bp with an N50 of 3.9 Mb (3 scaffolds).Annotation of protein-coding genes was performed using RATT, with Yarrowia lipolytica genome as reference. Other genetic elements (tRNA and transposable elements) were expertly added.
The genome of Yarrowia lipolytica strain H222 has been sequenced using Illumina Solexa HiSeq2000 technology with a paired-end library (275X, average insert of 400 bp.) and a mate-pair library (47X, average insert of 8 kb.). The raw reads were trimmed with Trimmomatic and cutadapt. The assembly was done using SOAPdenovo2 with a kmer of 77.The current draft genome sequence is composed of 17 scaffolds larger than 5 kb (all nuclear), for a total size of the nuclear DNA of 20,519,037 bp with an N50 of 3.9 Mb (3 scaffolds).Annotation of protein-coding genes was performed using RATT, with Yarrowia lipolytica genome as reference. Other genetic elements (tRNA and transposable elements) were expertly added.