GSE169264
Identification of mRNA clients of SYNJ2BP by RIP-seq
We discovered SYNJ2BP is an OMM-localized RNA binding protein. We identified the potential mRNA clients of SYNJ2BP by RIP-seq and validated by CLIP-qPCR. Overall design: The SYNJ2BP RIP was performed using the GenSeqTM RIP Kit and a SYNJ2BP antibody (HPA000866-100UL, Sigma-Aldrich) along with an IgG negative control. RNA-seq libraries were generated using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturer's instructions and the library quality was evaluated with BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). Library sequencing was performed on an illumina Hiseq instrument with 150 bp paired-end reads.