Response_of_myeloid_lineage_cells_to_cytokines-sc-2013-03-04T13:45:38Z-2557
Response_of_myeloid_lineage_cells_to_cytokines
This study is focused to find new genes involved in Insulin signalling in bone marrow derived macrophages upon treatment with IL-4 and LPS. We will use primary macrophages differentiated from bone-marrow derived hematopoetic stem cells; our aim is to analyze the transcriptome upon anti-inflammatory (IL-4) and inflammatory (LPS) treatment.  Total RNA was extracted with RNAEasy kit form Qiagen using manufacturer reccomendations and RIN values for all samples were between 9 and 10.
Goals: Study the changes in gene expression in the presence and absence of Insulin signaling in BMDM in response to  inflammatory and anti-inflammatory signals.  Purpose and scope: Describe the implication of  genes in this processes to know which genes are under control of Insulin pathways under different stimuli.