PRJNA761880
ChIP-seq data of sigma factor SigE and SigA in Synechocystis sp. PCC6803
Sigma factors are the subunit of bacterial RNA polymerase, which determines expressed genes by recognition of the promoter sequence. Housekeeping genes are transcribed by the primary sigma factor. Cyanobacteria, the oxygenic phototrophic eubacteria, have multiple alternative sigma factors to cope with various environmental changes other than the primary sigma factor (SigA). Non-essential variants highly homologous to the primary sigma factor are called group2 sigma factors. Model cyanobacteria Synechocystis sp. PCC6803 has multiple group2 sigma factors (SigB, C, D, E) other than SigA. Among cyanobacterial group2 sigma factors, SigE is unique because it alters carbon metabolism by expressing genes related to glycolysis, oxidative pentose phosphate pathway, and glycogen catabolism. Due to the high homology of the primary and group2 sigma factors, the consensus motif of SigE regulon remains elusive. Here we identified the direct target of SigA and SigE by chromatin immunoprecipitation sequencing (ChIP-seq) and revealed that SigE and SigA binding sites are substantially overlapping, but SigE also localizes on SigE dependent promoters with the unique consensus sequence.