P. persimilis transcriptome
Transcriptome of the predatory mite, Phytoseiulus persimilis
These are obtained sequences of expressed genes in the different immature and adult stages of the predatory mite, Phytoseiulus persimilis. Messenger RNA was extracted from 7mg of mites and used to produce a cDNA library. This library was submitted to the Genome Science Laboratory at NCSU for pyrosequencing with the 454 technology.
Much can be learned from the study of transcriptomes of mites, which can generate DNA-sequence information of genes to study different physiological processes. The predatory mite, Phytoseiulus persimilis, was selected as a model organism to conduct a transcriptome analysis with the 454 pyrosequencing technology. The objective of this project was to obtain DNA-sequence information of expressed genes from P. persimilis, with special interest in sequences corresponding to vitellogenin (Vg) and vitellogenin receptor (VgR) genes.