GSE124000
Transcriptome analysis of electroporated/GFP+ cells from embryonic mouse cortex E15.5 (E13.5 + 48 h), shRNA against circSlc45a4, mRNA Slc45a4 and scramble KD
We perturbed the expression levels of circSlc45a4 and mRNA Slc45a4 in developing mouse cortex (E15.5) by RNAi/shRNAs. 48 h post-electroporation the cells were isolated by FACS (GFP+), RNA was extracted with Trizol and polyA+ libraries were generated with Illumina TruSeq Stranded mRNA LT Sample Prep Kit. We find many significant changes in the transcriptomic profile after knockdown of circSlc45a4 and mRNA Slc45a4, that are distinct from each other. Overall design: Examination of 3 different shRNAs against circSLC45A4 and 2 different shRNAs against mRNA Slc45a4 vs. 1 scrambled siRNAs, 3 biological replicates per shRNA mix.