DRP002604
MiSeq analysis on the RT-PCR amplicon of HCV from human sera.
The study focused on the question of whether HCV/HIV coinfected hemophiliacs, who are suspected of being repeatedly exposed to unheated blood-related products, differ in the composition of hepatitis C viral quasispecies (genotypes and resistance mutations), from  HCV  monoinfected  non-hemophiliacs  with  or  without  a  history  of  whole  blood transfusion.  Twenty one  clinical  serum  samples were included in this study for the analysis on  the  HCV  core  and  NS3 protease  region  for  genotyping  and  the  NS3  protease  region  for  naturally  occurring resistance-associated  variants,  by  Illumina  MiSeq  deep  sequencing  .