ena-STUDY-JIC-13-12-2013-15:49:36:222-76
: Resistance gene enrichment (RenSeq) on gDNA and cDNA of Solanum lycopersicum Heinz 1706 and Solanum pimpinellifolium LA1589
RenSeq was developed to reannotate the full NB-LRR gene complement in Solanaceae and to identify novel sequences that were not picked up by the automated gene prediction software. Using 250-bp MiSeq reads after RenSeq on genomic DNA of Heinz 1706, we identified 105 novel NB-LRR sequences. Reannotation included the splitting of gene models, combination of partial genes to a longer sequence and closing of assembly gaps. Within the draft S. pimpinellifolium LA1589 genome, RenSeq enabled the annotation of 355 NB-LRR genes. The majority of these are however fragmented, with 5’- and 3’-end located on the edges of separate contigs. Use of RenSeq on cDNA from uninfected and late blight-infected tomato leaves allows the avoidance of sequence analysis of non-expressed paralogues.
RenSeq was developed to reannotate the full NB-LRR gene complement in Solanaceae and to identify novel sequences that were not picked up by the automated gene prediction software. Using 250-bp MiSeq reads after RenSeq on genomic DNA of Heinz 1706, we identified 105 novel NB-LRR sequences. Reannotation included the splitting of gene models, combination of partial genes to a longer sequence and closing of assembly gaps. Within the draft S. pimpinellifolium LA1589 genome, RenSeq enabled the annotation of 355 NB-LRR genes. The majority of these are however fragmented, with 5’- and 3’-end located on the edges of separate contigs. Use of RenSeq on cDNA from uninfected and late blight-infected tomato leaves allows the avoidance of sequence analysis of non-expressed paralogues.