GSE106193
Chem-seq data from 5'-biotin-DRB-labelled human U2OS cells
Confluent U2OS cells were released to serum-free medium, and continued to grow for 36 h (Per2-dLuc peak), then added biotin-DRB (40uM) treatment for 0.5, then fixed the cells, harvested genomic DNA by Strepavidin agarose beads, and conducted hi-seq sequencing Overall design: To overcome the poor quality of commercial Abs against CDK9 for the ChIP assay, we apply the small molecule-bound hi-seq assay to evaluate the global CDK9 association on the chromatin