GSE127207
Transcriptomes of pssu(z)12 mutant from phytophthora sojae P6497 at mycelium stage
Purpose and methods:Transcriptome profiling of Phytophthora sojae pssu(z)12 mutant T34 (lost 561bp by CRISPR/Cas9) mycelium (3-days old) were generated to find out the relationship between H3K27me3 and gene expression on Avr1b locus. Wild-type P. sojae P6497 was took as a comparative control. RNA-seq data was mapped using Tophat2, and gene expression data was generated by Cufflinks. Transcriptome profiles were displayed using IGV browser. Overall design: mRNA profiles of Phytophthora sojae pssu(z)12 mycelium(mu,3-days old)was generated by deep sequencing, in two replicates. Wild-type P.sojae P6497 RNA-seq data was accessed from NCBI (GSE116089).