WGS of Eimeria tenella Houghton strain-sc-2010-08-13
WGS of Eimeria tenella Houghton strain
Coccidiosis, caused by species of intracellular protozoan parasites belonging to the genus Eimeria (phylum Apicomplexa), remains one of the economically most important diseases in modern poultry production. The disease is caused by the replication within the intestine of the asexual and/or sexual stages of seven species of Eimeria (usually several species occur concurrently). The Houghton strain of Eimeria tenella was selected for WGS capillary sequencing to 8X genome coverage. This produced a highly fragmented assembly with the genome being assembled to ‘repeat-rich’ and ‘repeat poor’ segmented regions. To improve the assembly and increase the accuracy of base calling, we have employed second generation sequencing technologies. PCR-free Illumina sequencing reads are being generated for improving the assembly and for accurate base calling. . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Illumina sequencing of E. tenella Houghton to aid de novo assembly and also to improve base accuracy.