Aplysia californica transcriptome (heart and salivary gland)
Aplysia californica transcriptome (heart and salivary gland)
We developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.
Aplysia californica is one of key models to study cellular bases of behavior and plasticity. For a comparative approach we performed transcriptome analysis of heart and salivary glands of Aplysia using pyrosequencing approach. Specifically, we developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly. This data was used to compare to the Aplysia neuronal transcriptome