GSE168292
Androgen Deprivation Alters the Transcriptome of Endothelial Cells More Dramatically in Human Prostate Cancer Tissue than in Benign Prostate Tissue
Purpose: Examine the cell type specific (Epithelial, Endothelial, and Stromal) transcriptome profile between prostate primary tissue xenografts of prostate tissue tumor tissue and uninvolved tissue (named non-tumor hereafter) in response testosterone (T) deprivation.  Additional cell type specific untreated fresh tissue from both tumor and non-tumor samples is included for additional validation.  Related samples previously uploaded to GEO are found under accession number GSE145641. Method: Nude-Fox1nu mice were castrated and silastic tubing containing dry T was inserted providing “humanized” levels of circulating T.  5-8 pieces of fresh human prostate tissue was then inserted subcutaneously along the flank of the animal.  For samples not subjected to T-deprivation, after 30 days the animal was euthanized, and tissue was harvested.  For samples undergoing T-deprivation, after 30 days with humanized circulating T levels, T-deprivation was performed by surgical removal of silastic tubing after which serum T levels dropped below the level of detection within 24 hours.  Samples were then harvested at Pre T-deprivation time point, an early T-deprivation time point (3-days) or late T-deprivation timepoint (15-17 days) post-surgery.  Tissue from fresh human prostate or tissue from xenograft and was then enriched into endothelial, epithelial and the remaining stromal compartments using magnetic bead enrichment.  RNA-Seq was then preformed on cell types from both origins and the results compared to determine the cell type specific effects of T-deprivation on the human prostate transcriptome. Overall design: This data set contains 127 samples from 24 individuals across three cell types (Endothelial, Epithelial, and Stroma) across two tissue types (tumor and non-tumor) and in both Fresh prostate Xenografts and fresh prostate tissue.  Samples from fresh porstate xenografts were subjected to T-deprivation and harvested at one of three timepoints (Pre T-deprivation, Early T-deprivation (3 days), or late T-deprivation (15-17 days))