ena-STUDY-ETH-11-12-2018-13:26:28:777-63
Analysis of the genes transcriptionally regulated in the course of the general stress response in Sphingomonas melonis Fr1
We analyzed the transcriptomes of different general stress response key regulator knockout mutants after stress exposure and compared them to the wild-type strain. Thereby we aimed to identify the regulon of the alternative sigma factor EcfG and to find out how the regulators PhyR, SdrG and the seven GSR-activating histidine kinases (Paks) influence transcription. The RNA for transcriptome analyses was prepared from the respective Sphingomonas melonis Fr1 cultures in mid-exponential phase. The cultures were split and were either treated with the appropriate stress stimuli or were left untreated. The cells were harvested 1 h after the beginning of the treatment.
We analyzed the transcriptomes of different general stress response key regulator knockout mutants after stress exposure and compared them to the wild-type strain. Thereby we aimed to identify the regulon of the alternative sigma factor EcfG and to find out how the regulators PhyR, SdrG and the seven GSR-activating histidine kinases (Paks) influence transcription. The RNA for transcriptome analyses was prepared from the respective Sphingomonas melonis Fr1 cultures in mid-exponential phase. The cultures were split and were either treated with the appropriate stress stimuli or were left untreated. The cells were harvested 1 h after the beginning of the treatment.