PRJNA735599
Overexpression of actin induced transcriptional changes of genes from BmN cells infected with BmNPV Raw sequence reads
To gain insight into how actin regulated BmN cells genes transcription, BmN cells were transfected with pIZ-actin and pIZ/V5-His (as control), then were infected with BmNPV at 12 hour post transfection (h p.t.) Total RNA from cells transfected with pIZ/V5-His and pIZ -actin were isolated and purified using TRIzo reagent at 48 hour post infection(Invitrogen).