PRJNA385555
Elucidating adaptive evolution of Thermoanaerobacterium  aotearoense SCUT27/?ldh-G58 to high-sugar medium
In this work, we first report the successful isolation of a commercial Thermoanaerobacterium aotearoense SCUT27/?ldh-G58 via sequential batch fermentation with step-increased carbon concentration. The evolved isolate rapidly catabolized sugars under high concentrations and subsequently produced ethanol with good yield. Transcriptome sequencing was employed to elucidate the regulatory mechanisms involved in the tolerance of SCUT27/?ldh-G58 to high-substrate medium.