GSE118000
Transcriptomic analysis of isolated zebrafish podocytes and endothelial cells exposed to human APOL1 mRNA
We injected zebrafish embryos with human APOL1 mRNA, dissociated embryos at 4 days of age, and purified podocytes and endothelial cells with fluorescence activated cell-sorting. After sorting, mRNA was extracted from purified cells and used to generate cDNA libraries, which were sequenced on an Illumina HiSeq 2500. Overall design: Examination of two cell types exposed to one of 3 mRNA treatments (sham, APOL1 G0, APOL1 G2)