GSE67960
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells [seq]
Using an unbiased chemical biology approach, we discover harmine as a novel regulator of Treg/Th17 differentiation. Harmine enhances Treg differentiation (working in conjunction with low levels of exogenous TGFb) and inhibits Th17 differentiation. Analysis of global gene expression of Tregs generated using low TGFb + harmine reveals significant similarity to Tregs generated using high TGFb only and suggests relevance of harmine-engaged mechanisms to IBD. Overall design: Naïve murine CD4+CD62L+ T cells were stimulated with anti-CD3/28, transduced with control or Dyrk1a shRNA, and treated with harmine or control.