CB4856 resequencing
C. elegans Hawaiian strain CB4856 genomic sequencing
Strain CB4856 was obtained from the Caenorhabditis Genetics Center, and maintained at 20*C using standard culture methods (Brenner 1974). Genomic DNA was extracted by proteinase K digestion followed by two rounds of phenol-chloroform extraction, with an intermediate step of RNase A digestion in TE. Genomic DNA libraries were built using Illumina's standard paired-end protocol and 36x2 bp were sequenced on the Illumina Genome Analyzer II following the manufacturer's recommendations. The number of detected paired-end reads amounted to 25,443,382 with an 8.2x coverage over the sequenced N2 genome.
We used Illumina resequencing of the CB4856 (Hawaiian) strain of C. elegans for the genome-wide discovery of single-nucleotide polymorphisms (SNP) relative to N2 (Bristol) strain.