GSE99228
Early events during the interaction between wheat and cereal cyst nematode as revealed by transcriptomic analysis
Cereal cyst nematode (Heterodera avenae) can be attracted by wheat roots before infestation, while largely is unknown underlying this phenomenon. Here, we examined the transcriptional responses of both wheat roots and nematodes during the attraction stage by mRNA sequencing analysis (with and without reference genome, respectively). We found that consistent with their respective mobility, the immobile host wheat root only had 93 DEGs (27 up-regulated and 66 down-regulated), while the mobile plant parasitic nematode H. avenae reacted much more actively with 879 DEGs (867 up-regulated and 12 down-regulated). Among the DEGs, a number of wheat DEGs (most down-regulated) were involved in biotic stress pathways, while several putative effector genes (up-regulated) were found in the nematode DEGs. Results of the experiments demonstrated that nematode responds more actively than wheat during the attraction stage of parasitism, and the parasite responses mainly involved up-regulation whereas the host responses mainly involved down-regulation. Overall design: mRNA profiles of wheat roots gathered aroud by cereal cyst nematodes (treated wheat roots), wheat roots untreated with nematodes (control of wheat roots), nematodes gathering around wheat roots (treated nematodes) and nematodes untreated with wheat roots (control of nematodes); five to six wheat seedlings in each pool of wheat treatment or wheat control and 25,000~30,000 nematodes in each pool of nematode treatment and nematode control; in triplicate for each treatment or control.