HeLa-H3.3-tetramer-split
Global identification of (H3.3-H4)2 tetramer split positions by sequential ChIP-Seq in HeLa cells
Our previous study revealed that nucleosomal H3.3-H4 tetramers split at a ratio of about 8%-10% during each cell cycle. However, the genomic distribution pattern of H3.3 splitting events is unknown. Here we used an unbiased approach based on sequential ChIP and high throughput sequencing to investigate the splitting distribution in a dual inducible HeLa cell line.