GSE158094
Trajectory analysis quantifies transcriptional plasticity during macrophage polarization
Ctyokine-stimulated polarization and repolarization of murine bone marrow-derived macrophages (LPS + IFN?, IL-4); bulk RNA-seq timecourse and bulk ATAC-seq for selected timepoints and conditions Overall design: Bulk RNA-seq and bulk ATAC-seq was performed on mouse bone marrow-derived macrophages treated with either LPS + IFN? or IL-4 at multiple timepoints after cytokine-induced polarization and repolarization. Unpolarized macrophages were used as a control. Three biological replicates were performed for all bulk experiments; one biological replicate was performed for the 10X experiment.