GSE131305
Single-cell protein-expression map of bone marrow reveals stromal cell subset selection with stress
We identified CD73+ bone marrow stromal cells as hematopoietic niche candidates. To further validate our finding, we tested the functional role of CD73 during bone marrow transplantation using CD73-/- mice. We evaluated the transcriptome of transplanted WT hematopoietic stem and progenitor cells from WT or CD73-/- recipient mice and found changes in metabolic, cell cycle and signaling genes. Overall design: We isolated LKS (Lin-Kit+Sca1+) cells from bone marrow of WT and CD73-/- recipient mice by flow cytometry, collected RNA, and ran RNA-seq.