E-MTAB-10301
RNA-seq of murine ES cells with Lima1 knockout, murine EpiS cells with Lima1 overexpression and human iPS cells with LIMA1 overexpression
In chimera assays, murine naïve embryonic stem (ES) cells usually produce better chimeras than the more primed epiblast-derived stem (EpiS) cells. Overexpression of the cytoskeleton-associated protein LIMA1/EPLIN in EpiS cells improves the rate of successful chimeras, while the knockout of LIMA1 in ES cells results in a metabolic state reminiscent of EpiS cells. To investigate the effects of LIMA1 we performed RNA-seq in Lima1 loss-of-function murine ES cells and in Lima1 gain-of-function murine EpiS cells and human induced pluripotent stem cells.
In chimera assays, murine naïve embryonic stem (ES) cells usually produce better chimeras than the more primed epiblast-derived stem (EpiS) cells. Overexpression of the cytoskeleton-associated protein LIMA1/EPLIN in EpiS cells improves the rate of successful chimeras, while the knockout of LIMA1 in ES cells results in a metabolic state reminiscent of EpiS cells. To investigate the effects of LIMA1 we performed RNA-seq in Lima1 loss-of-function murine ES cells and in Lima1 gain-of-function murine EpiS cells and human induced pluripotent stem cells.