ena-STUDY-FISABIO-10-09-2019-14:57:41:009-341
The metagenomic analysis of formalin-fixed paraffin-embedded tumour and distal tissue from colorectal cancer patients reveals differences in the microbiome
A total of 98 samples from 49 patients diagnosed with Lynch syndrome and having developed colorectal cancer were collected for this study.From each patient, two formalin-fixed and paraffin embedded (FFPE) samples, collected from the surgically removed piece of the gut were obtained, one from the tumour itself and one from a distal section of non-affected tissue. For all samples, the metagenomic taxonomic and gene shot-gun analysis of the microbiota was carried out by a metagenomic approach, using a shotgun sequencing with the Illumina NextSeq with 150 bp x2 chemistry.After sequencing 94 samples were paired (tumour and distal tissue from the same subject) and two contained only the tumour sample, since the distal pieced failed in the sequencing, totalizing the 96 deposited samples.
A total of 98 samples from 49 patients diagnosed with Lynch syndrome and having developed colorectal cancer were collected for this study.From each patient, two formalin-fixed and paraffin embedded (FFPE) samples, collected from the surgically removed piece of the gut were obtained, one from the tumour itself and one from a distal section of non-affected tissue. For all samples, the metagenomic taxonomic and gene shot-gun analysis of the microbiota was carried out by a metagenomic approach, using a shotgun sequencing with the Illumina NextSeq with 150 bp x2 chemistry.After sequencing 94 samples were paired (tumour and distal tissue from the same subject) and two contained only the tumour sample, since the distal pieced failed in the sequencing, totalizing the 96 deposited samples.