ena-STUDY-University of Zagreb, Faculty of Food Technology and Biotechnology-12-10-2018-12:36:42:786-1168
Prokaryotic community structure during a holomictic event in marine meromictic lake
Rogoznica Lake (Croatia) is a typical example of marine meromictic  lake. Holomictic events are rare and happen after long periods of  stratification durin which oxygen consumption results in occurrence of  anoxic holomictic conditions in the whole water column. fast intrusion  of the nutrient rich bottom waters to the surface decreases the N/P  ratio because of P release from the bottom waters. In contrast, there  is P limitation in the surface water during spring and summer  stratification. During and after the appearance of anoxic holomixis,  the whole water column appears as N limited. This highly influences  biology of the lake. However, little is known about prokaryotic  communities under these conditions. Therefore, the aim of this study  was to determine the community structructure and the abundance of  prokaryotic organisms by quantitative PCR and high-throughput 16S rRNA  gene sequencing of DNA.
Rogoznica Lake (Croatia) is a typical example of marine meromictic  lake. Holomictic events are rare and happen after long periods of  stratification durin which oxygen consumption results in occurrence of  anoxic holomictic conditions in the whole water column. fast intrusion  of the nutrient rich bottom waters to the surface decreases the N/P  ratio because of P release from the bottom waters. In contrast, there  is P limitation in the surface water during spring and summer  stratification. During and after the appearance of anoxic holomixis,  the whole water column appears as N limited. This highly influences  biology of the lake. However, little is known about prokaryotic  communities under these conditions. Therefore, the aim of this study  was to determine the community structructure and the abundance of  prokaryotic organisms by quantitative PCR and high-throughput 16S rRNA  gene sequencing of DNA.