ena-STUDY-Key Laboratory of Environmental and Applied Microbiology, CAS-31-05-2021-09:14:06:277-534
the nrfA-type community structures were significantly influenced by corpse degradation and time, and were gradually differential with succession
As a high quality external nutrient input, corpse decay may pose threats (e.g. cadaverine, putrescine and ammonia) to environment, leading to nitrogen pollution. Nitrate or nitrite can be converted into nitrogen by denitrifying microbial community, and then reduce nitrogen contamination. And this process can influence temporal succession of the microbial community, and improve self-purification ability of ecosystem. However, there is few research to investigate how nrfA-encoding denitrifiers respond to carcass decomposition. Here, high-sequencing and physicochemical analysis were applied to describe the temporal turnover and succession of nrfA denitrifying microbial communities on soil. Our data showed nitrate nitrogen (NO3-N), ammonia nitrogen (NH4-N) and total phosphorus (TP) were increased significantly by cadaver degradation, compared with CG. The nrfA-type denitrifying microbial communities were also significantly shifted by corpse decay and time, and the decomposition process also accelerated the temporal turnover. Additionally, the nrfA-type community structures were significantly influenced by corpse degradation and time, and were gradually differential with succession. Network analysis revealed that most environmental factors were significantly related to dominant microorganism. Particularly, the primary impacting factors of nrfA denitrifiers were treatment (i.e. corpse decay), time, TP, and NO3-N, which indicated corpse and physicochemical properties together regulated the shape of these microbial communities. It also gives new sight to explore the effect and assess risk on soil microbial community caused by corpse decay.
As a high quality external nutrient input, corpse decay may pose threats (e.g. cadaverine, putrescine and ammonia) to environment, leading to nitrogen pollution. Nitrate or nitrite can be converted into nitrogen by denitrifying microbial community, and then reduce nitrogen contamination. And this process can influence temporal succession of the microbial community, and improve self-purification ability of ecosystem. However, there is few research to investigate how nrfA-encoding denitrifiers respond to carcass decomposition. Here, high-sequencing and physicochemical analysis were applied to describe the temporal turnover and succession of nrfA denitrifying microbial communities on soil. Our data showed nitrate nitrogen (NO3-N), ammonia nitrogen (NH4-N) and total phosphorus (TP) were increased significantly by cadaver degradation, compared with CG. The nrfA-type denitrifying microbial communities were also significantly shifted by corpse decay and time, and the decomposition process also accelerated the temporal turnover. Additionally, the nrfA-type community structures were significantly influenced by corpse degradation and time, and were gradually differential with succession. Network analysis revealed that most environmental factors were significantly related to dominant microorganism. Particularly, the primary impacting factors of nrfA denitrifiers were treatment (i.e. corpse decay), time, TP, and NO3-N, which indicated corpse and physicochemical properties together regulated the shape of these microbial communities. It also gives new sight to explore the effect and assess risk on soil microbial community caused by corpse decay.