GSE203167
Transcriptomic analysis of TCF-7 cKO CD8 T cells
T Cell Factor-1, encoded by TCF-7, is a transcription factor that plays an essential role during T cell development and differentiation. In this manuscript we utilized a pre-clinical model provided evidence that TCF-7 is dispensable for the anti-tumor response, and that TCF-7 suppresses key transcriptional factors Eomes and T-bet and molecules responsible for peripheral CD8 T cell cytolytic function. We discovered that TCF-7 regulates NKG2D expression on naïve and activated mouse CD8 T cells, and that peripheral CD8 T cells from TCF-7 cKO utilize NKG2D to clear tumor cells. We also provide evidence that TCF-7 regulates key signaling molecules, including LCK, LAT, ITK, PLC-y1, P65, ERKI/II, and JAK/STATs required for peripheral CD8 T cell persistent function.  Our data transcriptomic and protein data uncovered the mechanism of how TCF-7 impacting peripheral CD8 T cell inflammatory cytokine production, CD8 T cell activation, and apoptosis. Our pre-clinical model showed that CD8 T cells from TCF-7 cKO mice did not cause GVHD, but effectively cleared primary tumor cells. Overall design: We analyzed FACS purified CD8 T cells of 3 different  TCF-7 cKO and WT mouse labeled this group of samples as pre-transplanted samples. For post-transplanted samples 1 x10^6 CD3 T Cells from  TCF-7 cKO and WT mice were initially transplanted into lethally irradiated recipent Balb/c mouse and at day 7 post-transplant H2kb+ donor CD4 and CD8 T cells  FACS sorted  from the spleen of recipients into the Trizol.