GSE114543
M6A IP from BrU-labeled and fragmented nascent RNA.
M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA. Overall design: HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse). Whole-cell total RNA was extracted with Trizol, fragmented (3min at 95°C, 10 mM ZnCl2) and nascent RNA was purified with anti-BrdU during BrU-IP. Fragmented nascent RNA was subsequently immunoprecipitated with anti-m6A.