GSE159978
RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out
We recently identified variants in PRDM15 in affected individuals with proteinuric kidney disease. To determine the downstream pathways mediated by PRDM15 knock-down, PRDM15 knock-out immortalized podocyte cell lines were generated using CRISPR/Cas genome editing. Two different guide RNAs were utilized to generate two different cell lines, while a scramble guide RNA was utilized as control. We performed RNAseq and identified 151 differentially regulated genes, which coalesced upon pathways involved in renal development. Overall design: RNAseq in control and PRDM15 knock-out immortalized human podocyte cell lines