ena-STUDY-NETHERLANDS CANCER INSTITUTE-30-11-2017-14:13:37:383-108
WXS of mouse DNA using a human enrichment kit
Sequence data (RNA/DNA) of patient derived Xenografts (PDX)not only contains reads of human (tumor) origin but also of mouse (host) origin. We developed XenofilteR, a tool to computationally dissect human and mouse sequence reads from xenograft sequence data. One of the validation sets for the tool includes sequence data of mouse DNA enriched with a human enrichment kit. This to show that also in WXS data murine sequence reads are present and hybridize with the baits designed based on the human genome.
Sequence data (RNA/DNA) of patient derived Xenografts (PDX)not only contains reads of human (tumor) origin but also of mouse (host) origin. We developed XenofilteR, a tool to computationally dissect human and mouse sequence reads from xenograft sequence data. One of the validation sets for the tool includes sequence data of mouse DNA enriched with a human enrichment kit. This to show that also in WXS data murine sequence reads are present and hybridize with the baits designed based on the human genome.