GSE202712
Meg-01 cell line expression data after propionate stimulation
Platelets are produced by megakaryocytes, deriving from megakaryocyte erythrocyte progenitors (MEP) in the bone marrow. Increased megakaryocyte expansion across common autoimmune diseases was shown for rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and primary Sjögren's syndrome (pSS). In this context, we evaluated the role of the microbial-derived short chain fatty acid (SCFA) propionate on megakayriocytes in vitro. Using RNA sequencing we characterized the consequences of propionate exposure on the megakaryoblast cell line Meg-01. Overall design: The megakaryoblast cell line Meg-01 was stimulated with 500 µM sodium propionate for 48 hours and untreated cells served as controls. RNA was extracted using a RNeasy Mini kit (qiagen, USA) according to the manufacturer's instructions. The Illumina RNA sequencing (RNAseq) analysis was performed by Novogene Sequencing – Europe (UK, Cambridge Sequencing Center). Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on an Illumina platform.