GSE200940
Effect of cardiac specific depletion of Mrps5 on gene expression in heart at 12 weeks after gene knockout
To understand the molecular mechanism underlying Mrps5 function in the heart, we performed RNA sequencing of Mrps5cKO and Mrps5fl/fl hearts 12 weeks after tamoxifen induction. We chose this stage because the mutant hearts have just started to exhibit detectable defects and the changes in the transcriptome state would more accurately correlate with the onset of the phenotype. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Mrps5 fl/fl;MCM mouse hearts and Mrps5 fl/fl (control) hearts at 12 weeks after tamoxifen induced Mrps5 gene deletion. Each group has 3 biological replicates, the control group indicates as Ctrl_1, Ctrl_2, Ctrl_3,  and the cardiac specific Mrps5  knockout group indicates as cKO_1, cKO_2, cKO_3.