GSE116900
RNA Seq of wild-type and VPS35-D620N-expressing SH-SY5Y cells
SH-SY5Y were created to stably express wild-type or VPS35-D620N (CMV-promoter driven) using a lentiviral vector (Vira power). Non-transduced cells were eliminated with blasticidin resistance gene included in viral cassette Overall design: Wild-type VPS35 and VPS35 D620N-expressing SH-SY5Y cells were differentiated with 10uM all-trans retinoic acid for 7 days and RNA extracted using Qiagen RNEasy kit. There are 3 independent experiments (replicates 1-3)