ena-STUDY-UNIVERSITY OF WARMIA AND MAZURY-27-05-2019-13:38:27:432-496
The porcine adrenal cortex transcriptome reads obtained from eight RNA-seq libraries: 4 untreated samples (CTR) and 4 samples administrated by LPS from Salmonella Enteritidis (LPS)
Lipopolysaccharide (LPS) is a bacterial endotoxin which can be engaged in induction of inflammatory responses. LPS may also play a significant role in some neurodegenerative, oncological and metabolic disorders. The aim of the current study was to determine the effect of a low single dose of LPS from Salmonella Enteritidis, which does not result in any clinical symptoms of intoxication (subclinical lipopolysaccharide), on the transcriptome of porcine adrenal cortex cells. Hypothetically, comparable subclinical amounts of Salmonella spp. LPS may be found in the plasma of healthy humans and in Salmonella asymptomatic carrier state. The study was designed to examine subclinical LPS influence on gene expression level, long non-coding RNA profiles and alternative transcription control pathways, such as alternative splicing events and RNA editing sites. After 7 days of LPS in vivo administration at a dose of 5 µg/kg body weight, the adrenal cortex tissues were collected from LPS-treated and control 8-9 week old female pigs and stored until total RNA extraction. The used RNA-seq technology allowed to explore deep transcriptomic changes in spliceosome and editome, and to compare expression profiles of mRNAs and noncoding RNAs in control and LPS groups.
Lipopolysaccharide (LPS) is a bacterial endotoxin which can be engaged in induction of inflammatory responses. LPS may also play a significant role in some neurodegenerative, oncological and metabolic disorders. The aim of the current study was to determine the effect of a low single dose of LPS from Salmonella Enteritidis, which does not result in any clinical symptoms of intoxication (subclinical lipopolysaccharide), on the transcriptome of porcine adrenal cortex cells. Hypothetically, comparable subclinical amounts of Salmonella spp. LPS may be found in the plasma of healthy humans and in Salmonella asymptomatic carrier state. The study was designed to examine subclinical LPS influence on gene expression level, long non-coding RNA profiles and alternative transcription control pathways, such as alternative splicing events and RNA editing sites. After 7 days of LPS in vivo administration at a dose of 5 µg/kg body weight, the adrenal cortex tissues were collected from LPS-treated and control 8-9 week old female pigs and stored until total RNA extraction. The used RNA-seq technology allowed to explore deep transcriptomic changes in spliceosome and editome, and to compare expression profiles of mRNAs and noncoding RNAs in control and LPS groups.