GSE120246
MYC Targeted Long Noncoding RNA DANCR Promotes Cancer in Part by Reducing p21 Levels.
p493 RNA-seq in the MYC on and MYC off conditions Overall design: For RNA-seq analysis of MYC-ON versus MYC-OFF states, the P493-6 cells, which were engineered to have tetracycline-repressible ectopic MYC, were supplemented with 0.1 µg/mL tetracycline (Sigma) for 48 hours before harvesting for the MYC-OFF state. The RNA from these cells were compared with MYC-ON cells that had continuously expressed MYC for 48 hours grown contemporaneously with the MYC-OFF cells.