Transposon_directed_insertion_site_sequencing_of_ISS1_libraries_of_Streptococcus_equi_recovered_from_ponies_with_strangles-sc-4525
Transposon_directed_insertion_site_sequencing_of_ISS1_libraries_of_Streptococcus_equi_recovered_from_ponies_with_strangles
This project exploits TraDIS (transposon-directed insertion site sequencing) to identify genes required by Streptococcus equi (S. equi) to cause strangles in a susceptible natural host. The results of this project will provide new insights into how S. equi causes disease, highlighting novel targets with which to design better vaccines.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We hypothesise that surface proteins of S. equi, which can be targeted for vaccine design, play an important role in its ability to cause strangles in horses. Thus, our aim is to identify S. equi genes that are required for the infection of Welsh mountain ponies, greatly increasing our knowledge of this important equine pathogen.  Objectives are to infect 15 Welsh mountain ponies with three tagged ISS1 mutant library of S. equi and recover abscess material for sequencing; to identify ISS1 mutants in the challenge inoculum and abscess material recovered from ponies post-challenge; to determine the fitness of individual ISS1 mutants by comparing the population of mutants sequenced pre- and post-challenge.