GSE191294
mRNA-seq of worms who raised on control RNAi or prp-19 RNAi were untreated or treated with atp-2 RNAi
Mitochondria are the central hub of energy and metabolism of the cell,acting as one of the most important organelles of living cells.Under mitochondrial stresses, cells will trigger mitochondrial unfolded protein response (UPRmt) to maintain mitochondrial homeostasis.We have verified that splicing facotrs are required for the activation of UPRmt.Knocking down of splicing fator prp-19 could   supresse UPRmt activation.We analysis the differencial alternative splicing of worms on L4440 RNAi or prp-19 RNAi and untreated or treated with atp-2 RNAi, we found that inhibition of prp-19 interrupts splicing homeostasis of c.elegans. Overall design: Examination of differiencial alternative splicing and mRNA profile of worms. Worms grown on L4440 RNAi or prp-19 RNAi were untreated or treated with atp-2 RNAi.