ena-STUDY-WAGENINGEN UR-08-02-2021-10:24:27:801-665
Wageningen University Common Carp Genome Assembly
In this study an improved genome of Common Carp (Cyprinus carpio) is created. Oxford Nanopore long read sequence data and Illumina data was used for primary assembly. Subsequently, sequence errors were removed as much as possible using Illumina short reads for polishing. Finally, a chromosome level assembly was achieved by including HiC data per Dovetail Genomics procedures. The final genome assembly had a scaffold N50 of 28.5 Mbp and was 97% BUSCO-complete (Actinopterygii gene set). Further validation was done by applying linkage map information, which also allowed confirmation that the 50 largest scaffolds are mostly consistent with the 50 chromosomes usually found in the species.
In this study an improved genome of Common Carp (Cyprinus carpio) is created. Oxford Nanopore long read sequence data and Illumina data was used for primary assembly. Subsequently, sequence errors were removed as much as possible using Illumina short reads for polishing. Finally, a chromosome level assembly was achieved by including HiC data per Dovetail Genomics procedures. The final genome assembly had a scaffold N50 of 28.5 Mbp and was 97% BUSCO-complete (Actinopterygii gene set). Further validation was done by applying linkage map information, which also allowed confirmation that the 50 largest scaffolds are mostly consistent with the 50 chromosomes usually found in the species.