GSE193444
Intermediate filaments are regulators organismal lifespan in C. elegans
Purpose: Try to find out whether germline loss retards the normal somatic aging Methods: Employed transcriptome analysis in both young and aged germline-deficient animals and identified genes that are normally decreased during aging in wild-type (WT) worms but are well maintained in aged germline-deficient animals Results: Compared with D1, there were 1834 genes downregulated at D6, while only 441 genes showed increased expression Overall design: Wild type Caenorhabditis elegans and glp-1 mutants are 25°C-cultivated  to the D1 adult , and keep at 20°C  to D6. Then  total mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000