GSE112117
Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe
We used CLIP-Seq to determine the RNAs bound specifically to RNA binding protein Mei2 in early meiosis in fission yeast. We added a TAP tag to the C-terminal ends of two meiotic RNA binding proteins, Mei2 and Msa1. We used an untagged fission yeast strain as a negative control. These strains were nitrogen starved and allowed to progress into meiosis, after which they were harvested, lyzed and crosslinking immunoprecipitaion was performed. The RNAs purified after CLIP were sequenced Overall design: We determined the RNAs bound to immunoprecipitated Mei2-TAP and Msa1-TAP