E_multilocularis_transcriptome-sc-2010-10-19
E_multilocularis_transcriptome
Total RNA was subjected to depletion of mitochondrial transcripts using RNase H and 5'-phosphate dependent exonuclease, followed by in vitro amplification of mRNA. After fragmentation, the sequencing libraries of metacestode, cultured cells (aggregates), non-acitivated and activated protoscoleces of E. multilocularis were constructed. 54bp short paired-end reads was generated using Illumina Genome Analyzer.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
mRNA and miRNA transcriptome data sets have been produced using in vitro amplified RNA from tissues and different stages of Echinococcus multilocularis with Illumina sequencing platform. The data have been used for genomic annotation, comparative expession analysis of genes and discovery of miRNAs.