ena-STUDY-Centre for Marker Discovery and Validation (CMDV)-29-04-2019-14:39:04:398-468
Whole genome resequencing of MR297 and MRQ76 rice varieties using Illumina HiSeq 4000 sequencing technology.
The genomes of MR297 and MRQ76 were sequenced using Illumina HiSeq 4000 to gain insight into their genetic basis. Bioinformatics analysis was carried out to identify potential single nucleotide polymorphisms (SNPs) in both varieties. Total DNA of each variety was extracted from leaves of two weeks old germinated seedlings using Murray & Motou protocol (Murray & Thompson 1980), 27 and Sigma DNA extraction kit. The DNA samples were sent to the sequencing service provider and sequencing was performed using Illumina HiSeq4000 sequencing (Illumina, Inc. Sand Diego, CA, USA). The sequencing procedure was performed by following the standard Illumina protocol. The average sequencing depth was approximately 30X coverage.
The genomes of MR297 and MRQ76 were sequenced using Illumina HiSeq 4000 to gain insight into their genetic basis. Bioinformatics analysis was carried out to identify potential single nucleotide polymorphisms (SNPs) in both varieties. Total DNA of each variety was extracted from leaves of two weeks old germinated seedlings using Murray & Motou protocol (Murray & Thompson 1980), 27 and Sigma DNA extraction kit. The DNA samples were sent to the sequencing service provider and sequencing was performed using Illumina HiSeq4000 sequencing (Illumina, Inc. Sand Diego, CA, USA). The sequencing procedure was performed by following the standard Illumina protocol. The average sequencing depth was approximately 30X coverage.