ena-STUDY-NATIONAL INSTITUTE OF GENETICS-24-06-2018-16:29:52:528-1061
Clonal expansion and diversification of cancer-associated mutations in endometriosis and normal endometrium
Endometriosis is characterized by ectopic endometrial-like epithelium and stroma, of which molecular characteristics remain to be fully elucidated. We sequenced 107 ovarian endometriotic and 82 normal uterine endometrial epithelium samples isolated by laser-microdissection. In both endometriotic and normal epithelium samples, numerous somatic mutations were identified within genes frequently mutated in endometriosis-associated ovarian cancers. KRAS was frequently mutated in endometriotic epithelium, with a higher mutant allele frequency (MAF) accompanied by arm-level allelic imbalances. Analyses of MAF, combined with multiregional sequencing, illuminated spatiotemporal evolutions of the endometriosis and uterine endometrium genomes. We sequenced 109 single endometrial glands and found that each gland carried distinct cancer-associated mutations, proving how heterogeneous the genomic architecture of endometrial epithelium is. Remarkable increases in MAF of mutations on cancer-associated genes in endometriotic epithelium suggests that retrograde flow of endometrial cells already harboring cancer-associated mutations have selective advantages at ectopic sites, leading to the development of endometriosis.
Endometriosis is characterized by ectopic endometrial-like epithelium and stroma, of which molecular characteristics remain to be fully elucidated. We sequenced 107 ovarian endometriotic and 82 normal uterine endometrial epithelium samples isolated by laser-microdissection. In both endometriotic and normal epithelium samples, numerous somatic mutations were identified within genes frequently mutated in endometriosis-associated ovarian cancers. KRAS was frequently mutated in endometriotic epithelium, with a higher mutant allele frequency (MAF) accompanied by arm-level allelic imbalances. Analyses of MAF, combined with multiregional sequencing, illuminated spatiotemporal evolutions of the endometriosis and uterine endometrium genomes. We sequenced 109 single endometrial glands and found that each gland carried distinct cancer-associated mutations, proving how heterogeneous the genomic architecture of endometrial epithelium is. Remarkable increases in MAF of mutations on cancer-associated genes in endometriotic epithelium suggests that retrograde flow of endometrial cells already harboring cancer-associated mutations have selective advantages at ectopic sites, leading to the development of endometriosis.