GSE158785
Mechanical Regulation of Stem Cell Proliferation and Fate Decisions by their Differentiated Daughters
RNAseq analysis of suprabasal cells in the epidermis of control and K10rtTA;TRESpastin (which depolymerize microtubules) at E16.5 were performed. We observed the premature differentiation of the barrier and increased actomyosin contractility in suprabasal differentiated cells upon microtubule disruption compared to control, and proved the increased actomyosin contractility caused by microtubule depolymerization leads to basal stem cell hyperproliferation during epidermal development. Overall design: mRNA profiles of suprabasal differentiated cells in the epidermis of control and K10rtTA;TREspastin at E16.5