RNAseq_of_mouse_olfactory_sensory_neurons_populations-sc-3453
RNAseq_of_mouse_olfactory_sensory_neurons_populations
Transcriptomic analysis of two cellular populations of the mouse olfactory mucosa. 6 x 10,000 olfactory sensory neurons (OSNs) were captured by FACS. Three samples from a high OMP-expressing population and three from a low OMP-expressing population. The RNA from each sample was sequenced on the Illumina Hiseq platform.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The mouse olfactory mucosa (OM) is a complex tissue, composed by anatomically and molecularly distinct cell types. Of particular interest are the olfactory sensory neurons (OSNs), as they constitute the functional unit of theolfactory system. In preliminary FACS sorting experiments using OMP-GFP mice (which label OSNs) we observed the existence of 2 OSN populations, as defined by fluorescence intensity. To explore the molecular heterogeneity and define the molecular barcodes of these 2 populations, we aim to sequence pools of 10,000 cells originated from each of these populations. This experiment is the final one to complete a paper on single cell analyses of OSNs.