GSE119070
Loss of maternal and zygotic ezh2 reveals the function of Polycomb repression in maintenance of gene expression domain after the body plan is established in zebrafish
The goal of the ChIP-sequencing experiments was to assess the differences in occupancy of a selection of chromatin marks and chromatin associated proteins in absence of both maternal and zygotic Ezh2 in zebrafish embryos at 24 hours post-fertilization (hpf). The goal of RNA-sequencing experiments was to ass the effect of the absence of both maternal and zygotic Ezh2 on gene expression in zebrafish embryos at 0, 3.3, and 24 hpf and link these differences with the changes in epigenetic mark occupancy. Overall design: RNAseq of 0 hph, 3.3hpf, and 24 hpf Danio rerio wildtype and maternal zygotic ezh2 (MZezh2) mutant whole embyos (5 to 7 replicates); ChIPseq for Ezh2 in 24 hpf Danio rerio wildtype (2 replicates) and MZezh2 mutant (2 sample) whole embryos (both second replicates, Drosophila spike in chromatin was used to assess ChIP and library quality);  ChIPseq for H3K27me3 in 24 hpf Danio rerio wildtype (2 replicates) and MZezh2 mutant (2 sample) whole embryos (both second replicates, Drosophila spike in chromatin was used to assess ChIP and library quality);  ChIPseq for Rnf2 in 24 hpf Danio rerio wildtype (1 replicates) and MZezh2 mutant (2 sample) whole embryos;  ChIPseq for H3K4me3 in 24 hpf Danio rerio wildtype (2 replicates) and MZezh2 mutant (2 sample) whole embryos;  ChIPseq for H3K27ac in 24 hpf Danio rerio wildtype (3 replicates) and MZezh2 mutant (3 sample) whole embryos;