GSE129984
IKK in mesothelioma
perform gene expression silencing for both IKKs in AE17, AB2 and KPM1 cells using lentiviral shRNA pools. After validating gene silencing, RNA was extracted from both parental (shC) and daughter cells (shChuk, shIkbkb) and was send for RNAsequencing in order to define possible target genes, up or down regulated upon gene expression manipulation with lentiviral shRNA. These cell lines (parental and daughter) were evaluated in vitro for the role of IKKs in cell proliferation, survival and clonogenicity and in vivo for their impact in MPM development upon intrapleural injection in mice Overall design: The purpose of this study was to functionally examine the main NF-?B activating kinases IKKa and ???ß in mouse models of human and murine malignant pleural mesothelioma (MPM). First, we induced MPM in C57BL/6 and Balb/c mice by intrapleural injections of syngeneic AE17 and AB2 MPM cells respectively