GSE157837
Transcriptional profile of bone marrow-derived macrophages from Cd28 KO mice
Analysis of the transcriptome of bone marrow-derived macrophages from Cd28 KO mice.   Methods:  C57BL/6J (WT) and Cd28 KO (B6.129S2-Cd28tm1Mak/J) mice were used. Bone marrow-derived macrophages were obtained by flushing the femurs of 6- to 10-wk-old C57BL/6, and Cd28 KO mice, and culturing cells during 7 days in DMEM supplemented with 10% FCS and 50 mM 2-ME, containing human M-CSF (25 ng/ml), with cytokine addition every 2 days. Overall design: mRNA profiles of bone marrow-derived macrophages from Cd28 KO and wild type