GSE174451
Anti-aging effect of olmesartan on miRNA expression profile in smooth muscle cells
High-throughput sequencing technique was used to screen the miRNAs which has potential role in the aging delaying effect of olmesartan on human smooth muscle cells. We cultured human vascular smooth muscle cells and used the third to fifth generation cells as unaging cells (Zhengchang-1,-2,-3), natural culture to passage 10 to 15 as aging group samples (Bo-1,-2,-3), and cells treated with olmesartan for the same time as aging group cells as treatment group (bo-ao-1,-2,-3). After collecting cells of each group, RNA was extracted and reversed. After the quality inspection was qualified, miRNA sequencing was carried out. Finally, we confirmed that the miRNA expression profile of human vascular smooth muscle cells changed after olmesartan treatment. This study confirmed that miRNA plays a potential role in the aging process of human smooth muscle cells and that olmesartan may play a role in delaying aging by regulating miRNA expression profile. Overall design: Examination the miRNAs profiles in Olmesartan-treated human Senescent VSMCs