GSE112036
Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta.
Purpose: Understanding the Mechanism of Action of the Anti-Dandruff Agent Zinc Pyrithione against Malassezia restricta. Methods: The transcriptome profile of the ZPT-treated M. restricta cells compared to that of untreated cells were generated by RNA-Seq using Illumina HiSeq. Generated raw reads that passed quality filters were mapped to the reference genome. Mapped reads were counted by featureCounts in Subread package v1.4.3 and the relative transcript abundance was TPM-normalized. Results: A number of genes were differentially expressed in the ZPT-treated cells, which include genes involved in zinc transporter, mitochondirial function, TCA cycle, electron transport chain and lipase. Overall design: Transcriptome of zinc pyrithione (ZPT)-treated M. restricta cells (5, 10, 20 uM) vs. Transcriptome of nntreated M. restricta cells