Analysis_of_drug_resistant_parasites_generated_by_drug_pressure_or_CRISPR_Cas9_editing_-sc-5401
Analysis_of_drug_resistant_parasites_generated_by_drug_pressure_or_CRISPR_Cas9_editing_
To investigate mechanisms of drug resistance, we use either drug-selections or CRISPR/Cas9 editing to generate Plasmodium falciparum parasites that possess one or more mutations in their genome. In the case of drug selections, parasites grown in vitro are exposed to increasing concentrations of compound until a shift in drug susceptibility is observed. In the case of CRISPR-edited lines, the parasite is transfected with the Cas9 plasmid along with an appropriate donor template to engineer specific changes in the genome.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The purpose of the study is to identify causal mechanisms of resistance. This information will point to potential drug targets that have mutated to decrease drug susceptibility, or to more general resistance mechanisms (e.g. transporters) that are protective to the parasite. Candidate genes are validated by CRISPR/Cas9 editing to either introduce or remove mutations of interest.