PRJNA516070
Comprehensive dynamic transcriptome analysis at two seed germination stages in maize (Zea mays L.)
Seed germination, as an integral stage of crop production, directly affects maize yield and grain quality. However, the molecular mechanisms of seed germination remain unclear in maize. We performed comparative transcriptome analysis of two maize inbred lines, Yu82 and Yu537A, at two stages of seed germination. Expression profile analysis during seed germination revealed that a total of 3381 and 4560 differentially expressed genes (DEGs) were identified in Yu82 and Yu537A at the two stages. Transcription factors were detected from several families, such as the bZIP, ERF, WRKY, MYB, and bHLH families, which indicated that these transcription factor families might be involved in driving seed germination in maize. Prominent DEGs were submitted for KEGG enrichment analysis, which included plant hormones, amino acid mechanism, nutrient reservoir, DNA replication and repair, metabolic pathways, and ribosome. Of these pathways, genes associated with plant hormones, especially gibberellins, abscisic acid, and auxin may be important for early germination in Yu82. In addition, DEGs involved in amino acid mechanism and DNA repair showed significantly higher expression levels in Yu82 than in Yu537A, which indicated that energy supply from soluble sugars, amino acid metabolism, and elevated efficiency in the repair of DNA damage may contribute to early germination in Yu82. The present results provide novel insight into transcriptional changes and gene interactions in maize during seed germination.