PRJNA513496
Whole genome shotgun sequencing of Pseudomonas spp. isolated by its ability to degrade furfural
Seven strains of Pseudomonas spp. were isolated by its ability to use furfural as a sole carbon and energy source. Two of them were previously isolated from wastewater treatment facilities (Lee et al. 2016. Appl Biochem Biotechnol 178:76–90) and the remaining five were obtained by enrichment cultures of sludge samples from pulp mill wastewater. In order to elucidate the molecular basis of furfural biodegradation, we obtained the draft genome sequences of these strains. Briefly, genomic DNA from Pseudomonas spp. isolates named ALS1131, ALS1279, FFL24, FFL34, FF4, VN230, and VN231 were subjected to paired-end sequencing in a MiSeq platform with at least a 30X coverage. Then, several assemblers (including Spades, Megahit, IDBA-UD among others) were used for genome reconstruction and the best assemblies were subjected to reconciliation tools (such as Metassembler) along with an in-house guided-assembly strategy. The final assemblies were checked with QUAST and annotated with the DDBJ Fast Annotation Tool using the Cluster of Orthologous Groups approach. These genomes represent a novel genetic resource for improved tolerance and conversion of furan aldehydes suitable for biotechnological applications.