GSE208254
Transcriptional profiling of IRF8 KO B cell differentiation
To understand the gene expression programs regulated by IRF8 in B cells responding to LPS stimulation we generated IRF8 deficient B cells by CRISPR/Cas9.  IRF8 knockout and wild-type CD43- splenic B cells were isolated and directly used for RNA-seq (naive B cells) or simulated to differentiate with LPS, IL2, and IL5 for 3 days.  At this time activated Bcells and plasmablasts were sorted for RNA-seq. Overall design: IRF8 knockout B cells and WT B cells representing naïve, activated B cells, and plasmablasts were isolated in triplicate for RNA-seq