ena-STUDY-NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY-27-03-2019-09:37:29:315-69
Metagenomic analysis of skin-mucus bacterial microbiota from sea caged Atlantic salmon
The dense populations in Atlantic salmon (Salmo salar) aquaculture are beneficial for parasites, pathogenic bacteria and virus, and a rich environmental microbiota. While there are several metagenomic studies of the gut microbiota of the Atlantic salmon, few studies focus on the mucosal layer - an important defence against disease and illness. This study is an initial mapping of the bacterial microbiota of the skin-mucus of healthy Atlantic salmon in sea cages using a metagenomics approach covering seven variable regions of the 16s rRNA genomic region. Metagenomics analysis by Ion Torrent next generation sequencing enable detection of variation on species level, give fever slash calls and miscellaneous reads, and a good species richness. Samples were collected from healthy, sea caged Atlantic salmon to gather a general bacterial population to study the microbiome. Analysis have identified 246 OTUs at family level, as well as 10 unidentified OTUs. Flavobacteriaceae, Moraxellaceae and Colweilliaceae are generally the most abundant OUTs identified across all samples, but species richness varies amongst samples, especially occurrence of less abundant species.
The dense populations in Atlantic salmon (Salmo salar) aquaculture are beneficial for parasites, pathogenic bacteria and virus, and a rich environmental microbiota. While there are several metagenomic studies of the gut microbiota of the Atlantic salmon, few studies focus on the mucosal layer - an important defence against disease and illness. This study is an initial mapping of the bacterial microbiota of the skin-mucus of healthy Atlantic salmon in sea cages using a metagenomics approach covering seven variable regions of the 16s rRNA genomic region. Metagenomics analysis by Ion Torrent next generation sequencing enable detection of variation on species level, give fever slash calls and miscellaneous reads, and a good species richness. Samples were collected from healthy, sea caged Atlantic salmon to gather a general bacterial population to study the microbiome. Analysis have identified 246 OTUs at family level, as well as 10 unidentified OTUs. Flavobacteriaceae, Moraxellaceae and Colweilliaceae are generally the most abundant OUTs identified across all samples, but species richness varies amongst samples, especially occurrence of less abundant species.