DRP004006
Synthetic cvxIAA-ccvTIR1 pair
7-day-old seedlings grown in liquid MS media were treated with 0.1 ??M  IAA or cvxIAA. Total RNAs were extracted after 3 hours. Each RNA  sample was prepared from a pool of thirty whole seedlings. Three  independent RNA samples for each condition were used for RNA-seq  analysis. Library preparation were performed using Illumina TruSeq  Stranded mRNA LT Sample kit. The resulting barcoded libraries were  pooled and sequenced on Illumina NextSeq500 sequencing platform, and  75-bp single-end reads were obtained.