GSE125347
RNA sequencing of 32D clone 3 cells transfected with CXCR2 (pEGFP-N1-CXCR2) or an empty vector(pEGFP-N1)
Purpose: CXCR2 was transfected into 32D clone 3 cells simulating the granulocyte and macrophage progenitor cells(GMPs) to confirm the role of CXCR2 in the differentiation of GMPs to macrophage and dendritic cell progenitor cells Overall design: RNA of 32D clone 3 cells transfected with CXCR2 or an empty vector (both incubated with CXC1 and CXCL2 for 4 h) were generated by deep sequencing, using Illumina HiSeq 2500 instrument