GSE127906
Jump-seq: Capture and amplify 5hmC Sites Whole Genome-Wide
We developed an efficient and precise method, which we name as Jump-seq, to capture and amplify 5hmC signal whole genome-wide, bridge the gap between base resolution (TAB-seq) and economic cost (hmC-seal). Overall design: The principle is to label 5hmC by 6-N3-Glucose moiety and a hairpin DNA oligonucleotide carrying an alkyne group can be covalently connected to the azide-modified 5hmC via Huisgen cycloaddition (click) chemistry. The primer extension from the hairpin motif extends to the modified 5hmC site and will continue to “land” on the genomic DNA. 5hmC sites could be inferred from the DNA polymerase “landing” pattern at the 5-prime juncture.