GSE210221
Single cell evaluation of endocardial HAND2 gene regulatory networks reveals critical HAND2 dependent pathways impacting cardiac morphogenesis.
The basic-helix-loop-helix transcription factor HAND2 plays a critical role in endocardium to myocardium signaling during cardiac morphogenesis through its role within the NOTCH signaling pathway. Hand2 endocardial deletion (H2CKOs) results in embryonic lethality by E13.5 due to tricuspid atresia (TA) or double inlet left ventricle (DILV) with accompanying intraventricular septum defects, multiple septa, and hypo-trabeculated ventricles. In addition, H2CKOs also exhibit an increased density of coronary lumens. In order to understand the underlying regulatory mechanisms that cause these phenotypes, we undertook a single cell transcriptome analysis of E11.5 H2CKOs hearts. Overall design: Hand2fx/+Nfatc1Cre males were crossed with Hand2fx/fxR262RmTmG/mTmG females to generate embryos at E11.5. Cre status determined by presence or absence of GFP fluorescence. Rapid genotyping used to adetermine the status of Hand2 conditional allele. Whole hearts from control and H2CKO embryos were dissociated for single cell sequencing.