ena-STUDY-ENS Lyon-13-03-2017-10:56:10:009-347
Mapping-by-sequencing data for mutant allele sy447 (Oscheius tipulae)
The Oscheius tipulae strain PS2373 carries the recessive mutation sy447 in the CEW1 background (reference genome). sy447 is an allele of the locus iov-3, published in Dichtel-Danjoy et al., 2004 (PMID:14697357)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 42 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.
The Oscheius tipulae strain PS2373 carries the recessive mutation sy447 in the CEW1 background (reference genome). sy447 is an allele of the locus iov-3, published in Dichtel-Danjoy et al., 2004 (PMID:14697357)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 42 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.