GSE184627
T-bet Suppresses TGF-b-independent Gut-resident Memory CD8+ T Cell Differentiation [RNA-seq]
TGF-beta signaling is required for the differentiation of gut-resident memory CD8 T cells. Here, we showed that the deficiency of transcription factor T-bet partially rescued the differentiation of TGF-beta receptor deficient gut-resident memory CD8 T cells. Overall design: Congenically marked naïve P14 TCR transgenic CD8 T cells were adoptively transferred into sex-matched C57BL/6 recipients followed by LCMV Armstrong infection. P14 T cells with different phenotypes were FACS sorted from both spleen and small intestine intraepithelial lymphocyte compartment.