GSE122784
Single cell RNA sequencing of HSC and MPP in DNA damage tolerant deficient mutants
We performed scRNA-seq according to the CEL-seq2 protocol in order to define transcriptome changes in mouse hematopoietic stem cell containing LSKs in different mutants with defects in DNA damage tolerances. Overall design: Using FACS, we sorted 2-4 96 well plates for WT, Rev1-/-, PcnaK164R/K164R, and PcnaK164R/K164R;Rev1-/- LSK cells.