GSE135718
Multiomic Fermentation Using Chemically Defined Synthetic Hydrolysates Revealed Multiple Effects Of Lignocellulose-Derived Inhibitors On Cell Physiology and Xylose Utilization In Zymomonas mobilis
The conversion of sugars in lignocellulosic hydrolysates to bioethanol represents an industrially relevant system for understanding microbial physiology associated with production of bio-based fuels and chemicals. To this end we have developed a new version of synthetic hydrolysate (SynH) modeled on highly concentrated 9% AFEX-pretreated cornstove hydrolysate (ACSH) with and without lignocellulose-derived inhibitors (LDIs) added, termed SynH3 and SynH3- respectively. We profiled the cellular responses of xylose-utilizing Z. mobilis 2032 grown in both SynH3- and SynH3 via collection and analysis of multiple omics-based data (multiomics) including transcriptomics, proteomics, and metabolomics. Our study was focused on answering the following two questions. First, how does Z. mobilis respond to LDIs in SynH3 and how does this compare to our previous studies with E. coli in 6% ACSH SynH? Second, what is the potential cause for the poor xylose conversion in the presence of LDIs? Addressing these questions will provide critical information for engineering of Z. mobilis strains with improved productivities in lignocellulosic hydrolysates. Overall design: Samples consist of four time points with three biological replicates each of cells either grown in control medium SynHv3.8 (aka SynH3- which does not contain lignocellulose-derived inhibitors) or SynHv3.11 (aka SynH3 which does include lignocellulose-derived inhibitors)