GSE134675
Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle [OCT4-AID ATAC-seq]
The pioneer activity of transcription factors allows for opening of inaccessible regulatory elements and has been extensively studied in the context of cellular differentiation and reprogramming. In contrast, the function of pioneer activity in self-renewing cell divisions and across the cell cycle is poorly understood. Here we assessed the interplay between OCT4 and SOX2 in controlling chromatin accessibility of mouse embryonic stem cells. We found that OCT4 and SOX2 operate in a largely independent manner even at co-occupied sites, and that their cooperative binding is mostly mediated indirectly through regulation of chromatin accessibility. Controlled protein degradation strategies revealed that the uninterrupted presence of OCT4 is required for post-mitotic re-establishment and interphase maintenance of chromatin accessibility, and that highly OCT4-bound enhancers are particularly vulnerable to transient loss of OCT4 expression. Our study sheds light on the constant pioneer activity required to maintain the dynamic pluripotency regulatory landscape in an accessible state. Overall design: ZHBTc4 cells expressing Ypet-MD and mCherry-OCT4-AID were treated with doxycycline for 24 hours before treatment with different IAA regimes, stained with Hoechst, sorted for different cell cycle phases, and subjected to ATAC-seq