Phoronopsis viridis Transcriptome
Phoronopsis viridis Transcriptome
We developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.
Phoronids ('Phoronida'), commonly known as horseshoe worms, are a relatively small animal phylum: twenty species are known, in two genera, Phoronis and Phoronopsis. Phoronids are worm-shaped. They are found in all oceans and seas including the Pacific Ocean, North Atlantic Ocean, and western Mediterranean Sea. Here, using pyrosequencing approach we initiated transcriptome analysis of this organism. Specifically, we developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.