Pyrosequencing Cotton fiber ESTs
Comparative transcriptional profiling of cotton fibers in Gossypium hirsutum and Gossypium barbadense using EST pyrosequencing and microarray hybridization
In this study we characterized the fiber transcriptomes of the two cultivated cotton species, Gossypium hirsutum and Gossypium barbadense that were parental genotypes of a RIL mapping population used previously for phenotypic QTL and expression QTL mapping., We used 454 deep pyrosequencing to characterize cDNAs from developing fibers of the 2 species at two key developmental time-points; 10 and 22 days post anthesis, dpa. One run (4 times a quarter of a run) of 454 pyrosequencing (GS FLEX Ti technology) generated 690,000 reads (617,000 high quality reads) belonging to 4 different fiber cDNA libraries, G hirsutum, Gh, (var 'Guazuncho 2'), G barbadense, Gb, (var 'VH8-4602') at 10 and 22 dpa. A unigene set was assembled and annotated, and differential digital gene expression was assessed.
Comparative transcriptional profiling of cotton fibers in Gossypium hirsutum and Gossypium barbadense using EST pyrosequencing and microarray hybridization