ena-STUDY-CHINESE ACADEMY OF AGRICULTURAL SCIENCES-09-05-2017-16:48:18:284-516
S. italica LP1 resequencing
In order to identified the candidate gene, we used whole genome resequencing and MutMap [30] analysis. Equal amounts of DNA samples from 30 recessive individuals of the lp1 × Yugu1 BC1F2 population were mixed and used for DNA library construction. Whole genome resequencing was carried out on Illumina Hiseq 2500 platform using 150bp paired-end strategy.
In order to identified the candidate gene, we used whole genome resequencing and MutMap [30] analysis. Equal amounts of DNA samples from 30 recessive individuals of the lp1 × Yugu1 BC1F2 population were mixed and used for DNA library construction. Whole genome resequencing was carried out on Illumina Hiseq 2500 platform using 150bp paired-end strategy.