ena-STUDY-COMENIUS UNIVERSITY IN BRATISLAVA-07-01-2019-20:39:37:722-642
Characterization of Dev-CT57, new Autographivirinae bacteriophage infecting Cronobacter spp.
Cronobacter spp. are opportunistic pathogenic bacteria responsible for severe infections in neonates. Powdered infant formula was confirmed to be the source of infection in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study we characterized one closely related Cronobacter-specific bacteriophage from the GAP227virus group. The Dev-CT57 phage possessed broad host specificity as it infected 80% tested Cronobacter strains. According to the genome comparisons Dev-CT57 phage have hight level of similarity to the prototype GAP227 phage. A phylogenic tree of RNA polymerase (RNAP) gene from selected Autographivirinae representatives confirmed grouping of Dev-CD23 and GAP227 phages in one cluster, the other members of the cluster were the PP2, Phi80-18 and PhiR8-01 phages. Obtained results will contribute to our understanding biology and evolution of Autographivirinae phages.
Cronobacter spp. are opportunistic pathogenic bacteria responsible for severe infections in neonates. Powdered infant formula was confirmed to be the source of infection in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study we characterized one closely related Cronobacter-specific bacteriophage from the GAP227virus group. The Dev-CT57 phage possessed broad host specificity as it infected 80% tested Cronobacter strains. According to the genome comparisons Dev-CT57 phage have hight level of similarity to the prototype GAP227 phage. A phylogenic tree of RNA polymerase (RNAP) gene from selected Autographivirinae representatives confirmed grouping of Dev-CD23 and GAP227 phages in one cluster, the other members of the cluster were the PP2, Phi80-18 and PhiR8-01 phages. Obtained results will contribute to our understanding biology and evolution of Autographivirinae phages.