GSE160346
Genome-wide Transcriptional Profiling of Bacillus subtilis in response to valinomycin and CCCP
Valinomycin is a cyclic depsipeptide antibiotic produced by Streptomyces fulvissimus, and also known as K+ ion-selective ionophore, that has been studied and used in research for over half of century. The broad antimicrobial activity of valinomycin is reported as an effect of the disturbance of ionic equilibrium and transmembrane potential that may influence the whole organism and then cause fatal consequences. CCCP, carbonyl cyanide 3-chlorophenylhydrazone, is a member of the lipophilic weak acid class and acts essentially as an ionophore and can reduce the overall metabolic activity of the cell, causing severe defects of cell growth.  In this study, we investigated the global cellular response of Gram-positive model bacterium Bacillus subtilis upon exposure to valinomycin as well as CCCP by genome-wide transcriptional profiling using RNA sequencing technique, aimed to unveil the underlying regulatory network of the stress response. Overall design: Wild type Bacillus subtilis W168 cells at exponential phase of growth were treated with cell membrane-disturbing compounds, valinomycin and CCCP (carbonyl cyanide 3-chlorophenylhydrazone), respectively. With 10 min of induction, all samples were processed to undergo total RNA extraction, cDNA library preparation and then high-throughput RNA sequencing. The resulting sequencing raw data were analyzed in silico to reveal the differential gene expression in different treatments relative to non-treatment control.