GSE175805
Single cell sequencing to investigate the alteration of urea cycle in DFMO treated mouse
Single cell sequencing to investigate the alteration of urea cycle in DFMO treated mouse Overall design: We incubated 2*10^5 MC38 cells into the back of C57BL/6 mouse. And 7 days after incubation, we started gavage of DFMO or water(NC) daily for 14 days. Xenograft tumors were collected and digested into single cells, followed by Ficoll seperation to obtain immune cells. We mix cells from xenografts of 4 mice per group  for single cell sequence.