Developmental D. melanogaster poly(A)  RNA-Seq
Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
These data were produced for the modENCODE project (http://www.modencode.org/). For a description of the protocols and other experimental details please refer to the data distribution site http://www.modencode.org/Celniker.shtml. In accordance with NHGRI guidelines for community resource projects (http://www.genome.gov/), there is no restriction on the use or analysis of these data. However, the end-user is asked to respect a nine-month moratorium (from the public submission date) before submitting manuscripts describing analysis of these data. If the data producer has published any description of these data before the nine-month period, the moratorium ceases to apply and this accession page will be updated to note the appropriate citation.
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.