ena-STUDY-THE UNIVERSITY OF QUEENSLAND-28-12-2017-04:48:47:821-446
Short read coffee bean ripening transcriptome of the upper canopy
This study includes high depth Illumina short reads generated from three development stages of coffee bean transcriptome of the upper canopy, including green (immature), yellow (intermediate) and red (mature) stages. Three biological replicates were conducted for each stage. Illumina Hiseq 4000 platform was applied. A standard 18 x Truseq total RNA library preparation and Ribo-Zero kit was used. Finally, 2×150bp paired-end reads were generated. Barcode and adapter were trimmed. Quality trimming was also processed with PHRED score 20 and 0.01 and length cut from 40bp.
This study includes high depth Illumina short reads generated from three development stages of coffee bean transcriptome of the upper canopy, including green (immature), yellow (intermediate) and red (mature) stages. Three biological replicates were conducted for each stage. Illumina Hiseq 4000 platform was applied. A standard 18 x Truseq total RNA library preparation and Ribo-Zero kit was used. Finally, 2×150bp paired-end reads were generated. Barcode and adapter were trimmed. Quality trimming was also processed with PHRED score 20 and 0.01 and length cut from 40bp.