GSE135418
Epigenetic changes in human DIPG cells with histone H3K27M mutations [ATAC-seq]
We assessed changes in chromatin accessibility in H3.3K27M DIPG cell lines on knockdown of metabolic enzymes including HK2, GDH and IDH1 Overall design: Hexokinase-2 (HK2), Glutamate dehydrogenase (GLUD1) or insocitrate dehydrogenase (IDH1) were knocked down in H3.3K27M HSJD-DIPG007 cells. Genomic changes in chromatin accessibility were assessed between each shRNA and non-targeted NT controls.