GSE175838
RNA sequencing comparing in-vitro lentiviral CRISPR-Cas9 knockdown of Non-Canonical Polycomb Repressive Complex 1.6 members from a Kras G12D Trp53 -/- background mouse lung cancer cell line
The goal of this study was to compare expression profiles of mouse Kras G12D Trp53 -/- lung cancer cells that have inactivated ncPRC1.6 components to a ncPRC1.6 wild type condition Overall design: Each condition (Empty vs sg_ncPRC1.6 member (either sgMga, sgL3mbtl2, sgPcgf6, or sgL3mbtl2+sgPcgf6 cotransduced) is represented in  1 control Empty line in biological triplicate, 1 sgMga line in biological triplicates, 1 sgL3mblt2 line in biological triplicate, 1sgPcgf6 in biological triplicate, and 1 sgL3mbtl2+sgPcg6 co-transduced line in biological triplicate. ChiP-Seq comparing binding of various Non-Canonical Polycomb Repressive Complex 1.6 members and Pol II between WT MGA and MGA KO in cells derived from Kras G12D + Trp53 -/- mouse tumours.