Illumina_NA07340-X_chromosome
Sequencing flow-sorted X chromosomes from a human female sample (CEU:NA07340) using the Illumina Genome Analyzer
Chromosomes were prepared from a lymphoblastoid cell line (GM07340,      within the National Institute of General Medical Sciences Human Genetic       Cell Repository).       Approximately 1.3 million X chromosomes were flow-sorted to a purity      of ~90%. We generated an Illumina sequencing library with a              median insert size of ~160 bp following random fragmentation and             gel fractionation of purified chromosomal DNA. Paired-end sequencing       of this library was carried out using the Illumina Genome Analyzer.       We carried out purity-filtering (PF) to remove mixed reads, where two or       more different template molecules are close enough on the surface of       the flow-cell to form a mixed or overlapping cluster. No other filtering       of reads has been carried out prior to submission. The data set      contains approximately 278 million PF reads of 30-35 bp.
We have generated paired-end sequence data covering the X chromosomes      of a human female individual to a sequence depth of more than       40-fold using the Illumina Genome Analyzer. The DNA identifier for this       individual is CEU:NA07340.