GSE129251
RNA sequencing analysis of ex vivo expanded CD27+CD70- and CD27-CD70+ human regulatory T cells
Our study shows that after extended stimulation of human CD4+CD25+CD127- Tregs in vitro, a stable cell subset that downregulates CD27 and upregulates CD70 develops. Expanded CD27+CD70- Tregs display a gene profile which closely relates to the previously described “Treg gene signature”, whereas CD27-CD70+ Tregs express some genes associated with Treg gene signature and some genes overexpressed in conventional T cells. Overall design: Comparison of the transcriptome profile of CD27+CD70- and CD27-CD70+ human regulatory T cells isolated from healthy individuals and after having been in vitro-expanded for 4 weeks using polyclonal stimulation