E-MTAB-10573
Mouse white adipose tissue HaloREVERBa ChIP-seq
The circadian clock component REVERBa is considered a dominant regulator of lipid metabolism, with global Reverba deletion driving dysregulation of white adipose tissue (WAT) lipogenesis and obesity. However, a similar phenotype is not observed under adipocyte-selective deletion (ReverbaFlox2-6AdipoCre), and transcriptional profiling demonstrates that, under basal conditions, direct targets of REVERBa regulation are limited, and include the circadian clock and collagen dynamics. Under high-fat diet (HFD) feeding, ReverbaFlox2-6AdipoCre mice do manifest profound obesity, yet without the accompanying WAT inflammation and fibrosis exhibited by controls. Integration of the WAT REVERBa cistrome with differential gene expression reveals broad control of metabolic processes by REVERBa which is unmasked in the obese state.
The circadian clock component REVERBa is considered a dominant regulator of lipid metabolism, with global Reverba deletion driving dysregulation of white adipose tissue (WAT) lipogenesis and obesity. However, a similar phenotype is not observed under adipocyte-selective deletion (ReverbaFlox2-6AdipoCre), and transcriptional profiling demonstrates that, under basal conditions, direct targets of REVERBa regulation are limited, and include the circadian clock and collagen dynamics. Under high-fat diet (HFD) feeding, ReverbaFlox2-6AdipoCre mice do manifest profound obesity, yet without the accompanying WAT inflammation and fibrosis exhibited by controls. Integration of the WAT REVERBa cistrome with differential gene expression reveals broad control of metabolic processes by REVERBa which is unmasked in the obese state.