ena-STUDY-UNIVERSITY OF OXFORD-04-10-2021-10:25:40:421-2086
Characterisation of Airway Metagenome in Severe Asthma
Asthma is the commonest chronic lung disease. Corticosteroid-resistant severe asthma carries greatest morbidity and lacks targeted therapies; emerging evidence implicates airways infection in stable disease (particularly H. influenzae). Previous asthma metagenomic studies lack adequate phenotyping and sequencing depth for bacterial species-level identification, in this study we address this through metagenomic sequencing using Oxford Nanopore to analyse airway samples collected from highly phenotyped severe asthmatics during stable disease.
Asthma is the commonest chronic lung disease. Corticosteroid-resistant severe asthma carries greatest morbidity and lacks targeted therapies; emerging evidence implicates airways infection in stable disease (particularly H. influenzae). Previous asthma metagenomic studies lack adequate phenotyping and sequencing depth for bacterial species-level identification, in this study we address this through metagenomic sequencing using Oxford Nanopore to analyse airway samples collected from highly phenotyped severe asthmatics during stable disease.