GSE96070
ChIP-seq for Foxg1 in E14-15 cortex
The goal of the study was to identify the binding site of Foxg1 in wild-type cortex by performing ChIP-seq using a Foxg1 antibody. E14-15 cortical tissues were dissected, lysed and fixed. Chromatin was prepared by sonication. Sequences bound by Foxg1 protein was precipitated using a Foxg1 antibody. Sequencing was performed on Illumina Genome Analyzer II. Foxg1 binding peaks were called using MACS. Overall design: ChIP for Foxg1, followed by sequencing on Illumina Genome Analyzer II platform