GSE201736
Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities
Here we aim to decipher the actions of Irx5 in the regulation of obesity and metabolic abnormalities. We employed a mouse model homozygous for an Irx5-knockout (Irx5KO) allele and conducted droplet based single-cell RNA sequencing (Drop-seq) in the hypothalamic arcuate-median eminence (ARC-ME), which is the main control center for sensing and integrating feeding regulatory signals. Overall design: Drop-seq was perfomed on WT and Irx5KO adult mouse hypothalamic arcuate nucleus-median eminence. Micro-dissected tissues from 5-7 mice per each group were pooled together for single cell dissociation, followed by Drop-seq sequencing.