GSE185099
Rapid and efficient adaptation of the dTAG system in mammalian development reveals stage specific requirements of NELF
mRNA expression profile of late 2-cell mouse embryos cultured +/- dTAG-13 to degrade NELFB from the zygote stage. Overall design: Zygotes were retrieved from homozygouse Nelfb-dTAG females post natural mating with homozygous males. Zygotes were culture for 30 hours +/- 500nM dTAG-13 in KSOM medium, then collected for SMART-seq