GSE123921
Developmental arrest in response to reduced protein synthesis in C. elegans
Reducing overall biosynthetic capacity is one avenue for increasing health and lifespan; however, animals must be post-developmental in order to reap these benefits. When protein synthesis is reduced in newly hatched C. elegans, worms enter a temporary arrest state that promotes stress resistance and increases lipid stores. In order to determine the genetic underpinnings to this developmental arrest, we used RNA-seq to determine the transcriptional profile of animals arrested for 48hrs and 120hrs as L2 animals (via egl-45/EIF3A and rps-11/RPS11 RNAi) versus 24hr Control RNAi non-arrested animals. The gene expression differences provide insights into the arrested state and allow for comparison to gene expression changes in adult animals in order to determine if they are activating similar pathways. Better understanding these gene expression changes will allow future research into activating the positive benefits of these pathways independently from any negative consequences of reducing biosynthetic capacity. Overall design: Transcriptional profiles of animals arrested for 48hrs and 120hrs as L2 animals (via egl-45/EIF3A and rps-11/RPS11 RNAi) versus 24hr Control RNAi non-arrested animals.