PRJNA766155
To test indel efficiency of Engineered FnCas9  in HEK293T cell line by high throughput sequencing
Datasets of experiments performed on Engineered Cas9 to test improved editing efficiency in the HEK293T cell line. Plasmid vector expressing xxxCas9-T2A-GFP and gRNA for different locus was transfected in HEK293T cell and targeted amplicon sequencing was performed on genomic DNA isolated from GFP positive cells 48hrs after transfection.