GSE161707
Primary intestinal myofibroplast transcriptomic profiles upon the LPS stimulation vs PBS controls across embryonic development (E14.5) and adulthood.
The intestinal stroma provides a microenvironment for immune cells activation. Upregulated Toll-like receptor 4 (TLR4) in the intestinal epithelium is has key roles in necrotizing enterocolitis among premature infants. However, the involvement of stroma in excessive inflammation is yet to be investigated. To characterize the immature stroma, intestinal stromal cells were collected from the small intestines of embryonic day 14.5 (E14.5) and adult mice and treated with lipopolysaccharide and a global mRNA sequence analysis of differentially expressed transcripts was performed. TLR4 was expressed at lower levels in embryonic stromal cells than adult. CD14 expressed in flow CD11b+CD45+ stromal cells was identified as a key inducer for TLR mediated inflammatory signals. Embryonic stroma induced differentiation of monocytes into a higher proportion of CD11bhighCD11chigh mononuclear phagocytes (MPs), which have lower CD103 expression than adult stoma. Co-culture of inflamed embryonic stroma with LPS induced increased phagocytotic activity of MPs, which was markedly reduced following CD14 knock down. In summary, the immature intestinal stroma hyper activates inflammatory signals through CD14, producing a high proportion of CD11bhighCD11chigh MPs, which could lead to subsequent aberrant immune activation. Overall design: We compared primary intestinal myofibroplast transcriptomic profiles upon the LPS stimulation vs PBS controls across embryonic development (E14.5) and adulthood.