GSE182754
Transcriptional effects of HSV-1-ICP0 and Ad5-E4ORF3 expression in BLaER1 monocytes [Virulence factors]
We have identified that the viral virulence factors ICP0 from HSV-1 and E4ORF3 from Adenovirus5 can activate the MORC3 pathway of innate immune sensing. To study the global transcriptional effects of virulence factor sensing, we overexpressed mCherry or these virulence factors from a doxycycline-inducible lentiviral construct in BLaER1 monocytes of various genotypes and performed RNA-seq. Overall design: Monoclonal gene-deficient BLaER1 expressing mCherry or viral virulence factors in three independent experiments.