GSE184077
Effect of V. cholerae biofilm formation and hemolysin production on human macrophages
In this study we determined the gene expression of THP-1 derived macrophages during the interaction with V. cholerae wildtype cells, biofilm-deficient or hemolysin-deficient mutants. Overall design: V. cholerae wildtype, ?mshA?tcpA, ?hlyA or ?mshA?tcpA?hlyA was exposed to THP-1 derived macrophages in 12-well plates and RNA-samples were taken at different time points after start of the V.cholerae-macrophage interaction. Three independent biological replicates for each sampled time point are provided. As a control, samples containing only macrophages were taken.