ena-PROJECT-TUM-29-05-2012-10:57:13:086-6
Genome sequence of the Lactobacillus sanfranciscensis phage EV3
Phage  EV3 was isolated from sourdough fermentations and was demonstrated to be active on Lactobacillus sanfranciscensis. EV3 belongs to the Siphoviridae family (Caudovirales)  (morphotype B1). Its life cycle at 25C° lasts 3 h with a burst size of about 30 viral particles per infected cell. The genome is a double-stranded linear DNA molecule with a pac-type system. Phage spreading into sourdough did not adversely affect acidification and volume increase of the dough neither lactobacilli counts. Phage EV3 encodes a dextransase located in the phage lysis module that is supposed to be a lysogenic conversion gene. Sequence analysis of the prophage sourounding regions revealed that the EV3 integration site is in the tRNALeu gene locus of the L. sanfrancicensis genome.
Phage  EV3 was isolated from sourdough fermentations and was demonstrated to be active on Lactobacillus sanfranciscensis. EV3 belongs to the Siphoviridae family (Caudovirales)  (morphotype B1). Its life cycle at 25C° lasts 3 h with a burst size of about 30 viral particles per infected cell. The genome is a double-stranded linear DNA molecule with a pac-type system. Phage spreading into sourdough did not adversely affect acidification and volume increase of the dough neither lactobacilli counts. Phage EV3 encodes a dextransase located in the phage lysis module that is supposed to be a lysogenic conversion gene. Sequence analysis of the prophage sourounding regions revealed that the EV3 integration site is in the tRNALeu gene locus of the L. sanfrancicensis genome.