PRJNA515982
Whole genome Sequencing of Cas9-mediated corrected iPSCs.
Confirmation of the nuclease acivities at the On- and Off-target sites. Genomic DNA was extracted using G-DEXTM IIc genomic DNA extraction kit (iNtRON Biotech) according to manufacturer's protocols from each clone. To construct sequencing library, purified DNA was randomly fragmented, followed by 5' and 3' adapter ligation. Adapter-ligated libraries were then PCR amplified and gel purified. Libraries were subjected to sequencing using Illumina NovaSeq6000 at Macrogen (South Korea). The sequencing depth was 40X. Sequencing data was converted into raw data using illumine package bcl2fastq (ver. 2.20.0). Adapters are not trimmed away from the reads.