PRJNA526508
N. crassa H3K27me3 mutant hunt sequencing
A strain of N. crassa bearing two, silenced antibiotic resistance markers (NCU05173::hph and NCU07152::nat-1) in H3K27 methylated regions of the genome were exposed to UV mutagenesis and mutants defective in H3K27me-mediated silencing were selected on antibiotic containing media. The resulting mutants, which were in a Oakridge genetic background, were crossed to a genetically polymorhpic Mauriceville strain to allow for SNP mapping of the causative mutation. Progeny of these crosses that were resistant to hygromycin and/or nourseothricin (and therefore contained the resistance marker and the causative mutation to allow for expression) were pooled and sequenced. This project contains sequencing reads for the mutant progeny as well as the parental strains that were used to subtract any SNPs that were present in the parents.