PRJNA869679
Single cell sequencing after sonodynamic therapy
Low tumor mutational burden and absence of T cells within the tumor sites are typical characteristics of "cold immune tumors" that paralyze the immune system. The strategy of reversing "cold tumors" to "hot tumors" infiltrated a high degree of T cells to activate antitumor immunity has attracted lots of attention. Herein, we encapsulated mesoporous dopamine with M1 macrophage-derived vesicles and loaded them with the fluorescent dye Indocyanine Green (ICG) core-shell MPDA /ICG@M1NVs NPs to realize nanoparticles-mediated local photothermal-triggered immunotherapy. As expected, MPDA/ICG core integrates dual Phototherapy of photodynamic therapy and photothermal therapy, thus enhancing cancer cell apoptosis by producing higher hyperthermia. The results of in vitro and vivo experiments demonstrate that the synergy between M1NVs mediated immunotherapy and MPDA/ICG -induced Phototherapy generated a localized antitumor-immune response with an excellent cancer-killing effect under the presence of tumor-associated antigens. At the same time, the addition of ICG brings dual-mode imaging of tumor fluorescence imaging and photoacoustic imaging to realize the early detection and diagnosis of liver cancer cells.