PRJNA873258
Macrogene sequencing of rice soil in southern China supplement
The aim of this research was to determine the abundances of heavy metal resistance genes and antibiotics resistance genes in soil.The qualified DNA samples were split, and double-end libraries were constructed using Covaris M220 (China Genetics Ltd). Double-end sequencing was performed on Illumina Hiseq Xten (Illumina USA) at Majorbio Biomedical Technology Co. Paired Illumina reads were trimmed on the adapter and low-quality reads (length < 50 bp or quality value < 20 or N bases) were removed by fastp. Macrogenomic data were assembled using MEGAHIT (https: // github.com / voutcn / megahit), and the MetaGene was selected to predict open reading frames (ORFs). Representative sequences from the non-redundant gene catalog were aligned with the NCBI NR database. Taxonomic annotation was performed using Diamond (http://www.diamondsearch.org/index.php). The ARDB database (http://ardb.cbcb.umd.edu/) was selected to annotate antibiotic resistance genes.