GSE119511
Effects of HOTAIR RNAi on Transcriptomes in T47D Cells
We profiled transcriptomes in human breast cancer cell line T47D when the expression of HOTAIR was knockdown by the siRNA specific to an HOTAIR isoform HOTAIR-N (NR_047517). Overall design: T47D cells were cultured in RPMI1640.  HOTAIR-N-specific and control siRNAs were transfected into T47D cells using RNAiMAX. Total cell RNA was extracted at 48 hrs after transfection. Three independent transfections were carried out for each siRNA. The extracted RNA was processed for RNA-SEQ on Illumina HIGHSEQ 2500.