GSE128265
mRNA regions where 80S ribosomes pause during translation elongation in vivo interact with protein uS19, a component of the decoding site
80S-ribosome bound uS19 translatome-wide crosslinking sites in human mRNAs revealed by PAR-CLIP Overall design: We focused on determining the uS19 contacts with mRNAs in vivo by applying the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method to a stable HEK293 cell line inducibly producing FLAG-tagged uS19 and grown on a medium containing 4-thiouridine.