ena-STUDY-Lubec-30-11-2014-16:28:04:307-28
De novo assembly and functional annotation of Arion vulgaris trancriptome using RNA-seq
Arion vulgaris, commonly known as Spanish Slug is a slug pest belonging to the family Arionidae. It was ranked among the hundred worst invasive species in Europe that has rapidly spread through many parts of the continent mainly due to lack of biological limiting factors. In this study, we report the transcriptome data that represent the first investigation of gene expression profile in Arion vulgaris on genome-scale level. RNA sequencing and de novo transcriptome assembly of Arion vulgaris transcriptome were performed to provide expression dataset for functional annotation. Approximate number of 339 millions of reads were generated by Ilumina HiSeq 2000 technology that were processed by Trinity pipeline resulting in 136,406 unigenes with average length of 671.04 bp and N50 of 971 bp. Functional annotation using web platform Fastannotator assigned 36,948 genes to the records present in NCBI non-redundant database and 22,868 fo them to Gene Othology terms. Searching against Pfam database identified 21,651 of entries to have at least one domain and 2,336 of genes were assigned with EC numbers. We expect that our study will provide a dataset for further trancriptome and proteome analyses of Arion vulgaris as well as studying the factors underlying its environmental resistance.
Arion vulgaris, commonly known as Spanish Slug is a slug pest belonging to the family Arionidae. It was ranked among the hundred worst invasive species in Europe that has rapidly spread through many parts of the continent mainly due to lack of biological limiting factors. In this study, we report the transcriptome data that represent the first investigation of gene expression profile in Arion vulgaris on genome-scale level. RNA sequencing and de novo transcriptome assembly of Arion vulgaris transcriptome were performed to provide expression dataset for functional annotation. Approximate number of 339 millions of reads were generated by Ilumina HiSeq 2000 technology that were processed by Trinity pipeline resulting in 136,406 unigenes with average length of 671.04 bp and N50 of 971 bp. Functional annotation using web platform Fastannotator assigned 36,948 genes to the records present in NCBI non-redundant database and 22,868 fo them to Gene Othology terms. Searching against Pfam database identified 21,651 of entries to have at least one domain and 2,336 of genes were assigned with EC numbers. We expect that our study will provide a dataset for further trancriptome and proteome analyses of Arion vulgaris as well as studying the factors underlying its environmental resistance.