Genome_Wide_Subnuclear_Architecture_in_Antigenically_Selected_Lines_of_P_falciparum_-sc-2012-05-08T10:04:44Z-429
Genome_Wide_Subnuclear_Architecture_in_Antigenically_Selected_Lines_of_P_falciparum_
We have used chromatin conformation capture and its derivatives to  study the nuclear architecture in lines of P.falciparum that express distinct variant antigens. Chemical crosslinking of cells followed by proximity based ligation and the subsequent enrichment of ligated junctions allows us  to estimate contact probabilities for all genomic loci at a resolution  of 5Kb - 50Kb. We evaluate these contact probabilities in the  context of polymer folding models to deduce the scaling principles and  folding architecture of interphase P. falciparum chromosomes. We  consider evidence for spatial segregation of subtelomeric chromosomal  territories involved in var gene expression as well as the anchoring  of heterochromatic regions to the nuclear periphery. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Illumina sequencing of chromatin conformation capture and its derivatives is being carried out to study nuclear architecture in antigenically selected lines of Plasmodium falciparum.