GSE119369
Inhibition of cancer cell self-renewal through an epigenetic HDACi-histone H1.0 axis.
Identification of HDACi-responsive, H1.0-dependent genes inhibiting self-renewal of HCC1569 breast cancer cells. Overall design: RNA-seq analysis of HCC1569 breast cancer cells containing an inducible H1.0-targeting shRNA, treated with HDACi (JNJ-26481585). RNA was collected at various time points after addition of HDACi (or DMSO as a control). Two conditions were compared: cells treated with doxycycline to induce expression of the shRNA (DOX), and uninduced cells (NT).