GSE97697
RNA seq analysis of pathogen-induced transcriptome changes in tomato plants overexpressing the Arabidopsis ELP4 gene
The goal of the RNA seq was to investigate the transcriptome changes induced by Pseudmonas syringae pv. tomato J4 in wild-type tomato 'Moneymaker' and transgenic 'Moneymaker' overexpressing the Arabidopsis ELP4 (AtELP4) gene. Results showed that P. syringae pv. tomato J4 induced dramatic transcriptional changes in both the wild-type and transgenic tomato plants.  Interestingly, a group of defense genes including PR-5x, Pti5, PR1b1, and CHI3/9/14/17, which are associated with resistance to the hemibiotrophic bacterial pathogen Ralstonia solanacearum, were induced to higher levels in the AtELP4 transgenic tomato than in the wild type at 8 and 24 hr after P. syringae pv. tomato J4 infection.  These results indicate that overexpression of AtELP4 in tomato leads to faster and/or stronger induction of some defense genes. Overall design: Three replicates with leaves from 6 plants per genotype were collected at 0, 8, and 24 hr after P. syringae pv. tomato J4 infection. Leaf tissues were collected and subjected to total RNA extraction.  After extraction, RNA concentration was determined on a NanoDrop Spectrophotometer (Thermofisher Scientific, Waltham, MA) and sample quality was assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).  The RNA samples from the three biological replicates were pooled and equal amounts of RNA from the pooled samples were used for RNA seq analysis.