ena-STUDY-UNIVERSITY OF BRITISH COLUMBIA-30-08-2017-22:55:33:461-260
In the first part of the experiment, naphthalene contaminated surface sediments, where effluent from the coal-tar contaminated groundwater surfaces, were incubated in situ with either 13C-labeled or natural abundance 13C-naphthalene at various dosing regimes. The dosing regimes correspond with 3 doses in a 24 hr timespan; 5 doses in a 72 hr timespan and 8 doses in 8 days. Shotgun metagenome libraries were prepared in triplicate from all 13C-labeled sediments following the enrichment for 13C-labeled DNA. Sediments fed natural abundance 12C-naphthalene were not replicated, but where exposed to the identical handling as DNA extracted from 13C-labeled sediment. In the second part of the experiment, groundwater was sampled from pristine ('upstream') and contaminated monitoring wells ('shallow','mid' and 'deep'). DNA was extracted directly from filters (5L of groundwater per well) and shotgun metagenomes were sequenced. This data corresponds with metaproteomic data from Hanson et al., 2015.
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