ena-STUDY-Institute of Cytology & Genetics of SB RAS-28-10-2021-09:34:16:124-450
SCA and SAA are short, highly hydrophylic proteins accumulated in the cotyledons and seed axis, respectively, of a dry pea (Pisum sativum L.) seed. Using high throughput sequencing data available in public databases we nominated coding sequences residing in the relevant region of the pea genome as candidates for the SCA and SAA genes, and sequenced them in a number of pea accessions using original primers.
SCA and SAA are short, highly hydrophylic proteins accumulated in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. It was used as a useful genetic and phylogeographical marker still requiring electrophoretic analysis while the DNA sequence of corresponding SCA gene remained unknown. Using high throughput sequencing data available in public databases searched for candidates for the SCA and SAA genes among coding sequences residing in the relevant region of the pea genome, and sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (both latter albumins being electrophoretically monomorphic). DNA amplification using primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease Hind II made SCA a convenient CAPS marker in peas.
SCA and SAA are short, highly hydrophylic proteins accumulated in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. It was used as a useful genetic and phylogeographical marker still requiring electrophoretic analysis while the DNA sequence of corresponding SCA gene remained unknown. Using high throughput sequencing data available in public databases searched for candidates for the SCA and SAA genes among coding sequences residing in the relevant region of the pea genome, and sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (both latter albumins being electrophoretically monomorphic). DNA amplification using primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease Hind II made SCA a convenient CAPS marker in peas.