ena-STUDY-NTNU-13-01-2015-09:47:06:067-306
The microbiota of developing cod larvae
The submitted dataset consists of pyrosequenced barcoded v4 16S rDNA amplicons representing bacterial communities associated with developing cod larvae, rearing water, and live feed samples. Cod larvae were reared with two different feeding regimes (copepods or rotifers).  Sampling of larvae and rearing water was carried out at 8, 17, 32, and 61 dph for two rearing tanks; one for each feeding regime. Each live feed culture was sampled once; the copepods at 8 dph, the rotifers at 17 dph, and Artemia culture at 32 dph. Total DNA from individual larvae, water and live feed samples were used as templates in PCR reactions. The V4 region of the 16S rRNA gene was amplified using a nested PCR protocol as described in Bakke et al. 2013, Environmental Microbiology Reports 5(4), 537-548. Two pooled amplicon libraries were generated (one for each rearing tank/feeding regime) and each of them was sequenced on one half of a 454 plate with a GS FLX instrument at the Norwegian Sequencing Centre.
The submitted dataset consists of pyrosequenced barcoded v4 16S rDNA amplicons representing bacterial communities associated with developing cod larvae, rearing water, and live feed samples. Cod larvae were reared with two different feeding regimes (copepods or rotifers).  Sampling of larvae and rearing water was carried out at 8, 17, 32, and 61 dph for two rearing tanks; one for each feeding regime. Each live feed culture was sampled once; the copepods at 8 dph, the rotifers at 17 dph, and Artemia culture at 32 dph. Total DNA from individual larvae, water and live feed samples were used as templates in PCR reactions. The V4 region of the 16S rRNA gene was amplified using a nested PCR protocol as described in Bakke et al. 2013, Environmental Microbiology Reports 5(4), 537-548. Two pooled amplicon libraries were generated (one for each rearing tank/feeding regime) and each of them was sequenced on one half of a 454 plate with a GS FLX instrument at the Norwegian Sequencing Centre.