ena-STUDY-VETMEDUNI-04-05-2016-12:20:41:702-2
Transcriptome sequencing reveals novel candidate genes for Cardinium hertigii-caused cytoplasmic incompatibility
Endosymbionts that infect arthropods and cause cytoplasmic incompatibility (CI) manipulate the reproduction of their hosts through “modification” of sperm and “rescue” of affected sperm in symbiont-infected eggs. The molecular mechanisms responsible for CI are still unknown. To find candidate symbiont genes involved in CI, we analyzed gene expression by transcriptome sequencing of CI-inducing Cardinium hertigii in male and female host parasitoid wasps, Encarsia suzannae. Three biological replicates of male and female pools (~ 400 wasps) were used for RNA extraction. Libraries were sequenced using Illumina HiSeq technology.
Endosymbionts that infect arthropods and cause cytoplasmic incompatibility (CI) manipulate the reproduction of their hosts through “modification” of sperm and “rescue” of affected sperm in symbiont-infected eggs. The molecular mechanisms responsible for CI are still unknown. To find candidate symbiont genes involved in CI, we analyzed gene expression by transcriptome sequencing of CI-inducing Cardinium hertigii in male and female host parasitoid wasps, Encarsia suzannae. Three biological replicates of male and female pools (~ 400 wasps) were used for RNA extraction. Libraries were sequenced using Illumina HiSeq technology.