23614:01b2bc0f-0958-43a2-b70f-8290484e3d0c
Benthic microbial communities of Fram Strait
Using TV-guided multicorer, sediment samples were  collected in Fram Strait during the Polarstern cruise PS99.2 (June 24th – July 16th 2016) from Greenland shelf to west coast of Spitsbergen. For assessing bacterial and archaeal community composition, a slurry of subsampled uppermost centimeter of each core were filtered with peristaltic pump through 0.22 µm membrane. The extracted genomic DNA was isolated and the hypervariable V4-V5 (515F - 926R) region of the 16S rRNA was amplified and sequenced using Illumina MiSeq platform in a 2 × 300 bp paired-end run.