GSE162858
Extensive epigenetic priming in multipotent progenitors precedes lineage specification in B-lymphocyte development.
B-lymphocyte development is dependent on the interplay between the chromatin landscape and lineage specific transcription factors. It has been suggested that B-lineage commitment is associated with major changes in the nuclear chromatin environment proposing a critical role for lineage specific transcription factors in the formation of the epigenetic landscape. In this report we have used chromosome conformation capture in combination with ATAC-seq analysis to enable highly efficient annotation of both proximal and distal transcriptional control elements to genes activated in B-lineage specification. A large majority of these genes were annotated to at least one regulatory element with an accessible chromatin configuration in multipotent progenitors. Furthermore, the majority of binding sites for the key regulators of B-lineage specification, EBF1 and PAX5,  occurred in already accessible regions. EBF1 did, however, cause a dynamic change in ATAC-accessibility and was critical for an increase in distal promoter-enhancer interactions as well as high deposition of chromatin modulating co-factors associated with the establishment of the B-lineage program. Our data unravel an unappreciated level of epigenetic priming at regulatory elements annotated to lineage restricted genes and provide insight into the mechanisms by which lineage specific transcription factors act via already accessible elements in lineage specification. Overall design: Murine B-cell progenitor cells of Wt, Ebf1-KO, E2A-KO or Pax5-KO genotype or 230-238 B-cell progenitor cell line were  were subject to ATAC-seq, ChIP-seq and PLAC -seq analysis. ChIP-seq was performed as single or duplicate experiments, ATAC- and PLAC-seq as duplicates.