GSE132361
miRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME)
We report the miRNA profiles of  macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LPS/pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling either 72 h (RAW264.7) or 144 h (TIME). Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of either LPS (1 µg/mL; from E. coli serotype 0111:B4) for cell line RAW264.7 or the cytokines IL-1ß, TNF-a, and IFN-? each in a concentration of 5 ng/ml for cell line TIME. Overall design: miRNA profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) supplemented with unsaturated fatty acids and exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina HiScanSQ.