GSE172496
Specific endothelical cells govern nanoparticle entry into solid tumours
We sequenced the transcroptome of isolated nanoparticle positive and nanoparticle negative tumour endothelial cells from mouse 4T1 mammary tumours Overall design: Tumour endothelial cells were isolated from 2 weeks old 4T1 tumours in female Balb/c mice using immunomagnetic isolation for CD45-, and CD31+ cells. Nanoparticle positive and negative tumour endothelial cells were then sorted by FACS to generate a nanoparticle positive endothelial cell group and a nanoaprticle negative endothelial cell group. This was done in 3 different tumours. RNA was isolated from the cells and processed for sequencing.