ena-STUDY-ETH ZURICH-11-06-2018-12:31:49:783-3822
Soil prokaryotic communities (16S rRNA gene) in the mine waste contaminated Ogosta floodplain.
Soil prokaryotic communities were studied along a 700 m long transect exhibiting a mine waste contamination gradient associated with extreme levels of arsenic, lead, copper, and zinc. Therefore, 16S rRNA gene amplicon sequencing was performed on the Illumina MiSeq. The amplicons were obtained with primers based on the sequences of the primer U519F (Wang and Qian, 2009) and R806 (Caporaso et al., 2011).
Soil prokaryotic communities were studied along a 700 m long transect exhibiting a mine waste contamination gradient associated with extreme levels of arsenic, lead, copper, and zinc. Therefore, 16S rRNA gene amplicon sequencing was performed on the Illumina MiSeq. The amplicons were obtained with primers based on the sequences of the primer U519F (Wang and Qian, 2009) and R806 (Caporaso et al., 2011).