GSE203457
Whole-genome CRISPR screening identifies N-glycosylation as an essential pathway and a potential novel therapeutic target in CALR-mutant MPN (RNA-Seq).
RNA-sequencing of megakaryocyte-erythroid progenitors of CALRdel52/+ MxCre mice treated with either vehicle or 2-deoxy-D-glucose for 21 days. Overall design: FACS sort of in vivo treated megakaryocyte-erythroid progenitors using the following markers: CD3- CD5- B220- Gr-1- CD11b- Ter119- Sca-1- c-Kit+ CD34low FcgRlow.