GSE136108
16S rDNA  Amplicon Sequencing Analysis of Polystyrene Microplastic Exposed Zebrafish Intestine
Microplastics (MPs) as widespread contamination pose high risk for aquatic organisms.Intestinal microbiotahas have high interaction with immune system of host body. In this study, intestinal microbiota of zebrafish after  Polystyrene (PS-MPs) exposure were characterized by 16S rDNA amplicon sequencing. We found that 100nm and 200µm PS-MPs exposure significantly increased diversity of intestinal microbiota and all the three sizes of PS-MPs increased abundance of pathogenic bacteria. Overall design: Detection of intestine microbes were conducted in zebrafish followed treatment of MPs with different size (100nm, 5µm and 200µm). For each assay, three individual trials were taken.