PRJNA261741
Whole transcriptome sequencing of transient HEK 293T transfectants of human APOBEC 3A, 3G or 2 cDNAs
To examine the effect of APOBEC 3A, 3G or 2 on gene expression, human HEK 293T cells were transiently transfected for two days with empty (vector alone) plasmids or mammalian expression plasmids bearing ORFs of the genes. Cells were transfected in triplicates. RNA was extracted and analyzed using the Illumina HiSeq 2000 platform and TruSeq Stranded Total RNA with Ribo-Zero sequencing library preparation method