GSE160125
I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells I
The adult Drosophila intestinal epithelium is a model system for stem cell biology, but its utility is limited by current biochemical methods that lack cell type resolution. We describe a new proximity-based profiling method that relies upon a GAL4 driver, termed intestinal-kickout-GAL4 (I-KCKT-GAL4), exclusively expressed in intestinal progenitor cells. This method used UV cross-linked whole animal frozen powder as its starting material to immunoprecipitate the RNA cargoes of transgenic epitope-tagged RNA binding proteins driven by I-KCKT-GAL4. Using I-KCKT-GAL4 based enhanced CLIP datasets provided here we mapped the RNA targets of a selective RNA binding protein, Fragile Mental Retardation Protein (FMRP). Overall design: Identification of RNA targets of FMRP using enhanced CLIP.