Ta7BS_sequence_assembly
Delimiting a translocation between wheat chromosome arms 7BS and 4AL with second generation sequencing
This study used DNA isolated from cytogenetic stock with a deletion of the long arm of chromosome 7B by flow sorting of chromosomes.  Short-read data was generated on the Illumina Genome Analyzer II and Illumina HiSeq 2000 to a coverage depth of 30x, then assembled using Velvet and scaffolded based on synteny with Brachypodium distachyon using a genome-zipper approach.  Syntenic build is publicly available at www.wheatgenome.info.
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have previously described reduced genome sequence complexity by isolating chromosome arm 7DS followed by sequencing and assembly (SRA025100.1). This study is applying the same technique to chromosome arm 7BS, followed by an analysis to delimit a translocation on this chromosome arm.  The 7BS assembly represents approximately 49% of the chromosome arm.