PRJNA558177
Fosmid clone with cellulose degrading gene prepared from metagenomic DNA of degrading wood samples from Sambhar Lake
To identify novel endoglucanases and beta glucosidase gene(s) from extreme halophiles of Sambhar Lake in Rajasthan a fosmid library of ~40000 clones was prepared. Selected positive clones were sequenced either by sub-cloning (EG14) followed by end sequencing using primer walking approach (EG14); or direct sequencing of fosmid DNA (EG3, EG35, EG78, and BG1) on NGS platform (ION S5).