GSE128929
Transcriptome analysis of PRRSV Nsp1 overexpressed 3D4/31 cells
This study investigated the immunological function of PRRSV Nsp1 by ectopic expression of PRRSV Nsp1 in 3D4/31 cell line. Identifying the functional role of PRRSV Nsp1 associated with host cell modulation may provide better knowledge about the pathogenesis of PRRS (Porcine reproductive and respiratory syndrome). Overall design: A macrophage cell line, 3D4/31, was transfected with pBabepuro (Mock), PRRSV NSP1-pBabepuro (PRRSV NSP1), PRRSV NSP1 alpha-pBabepuro (PRRSV NSP1a), or PRRSV NSP1 beta-pBabepuro (PRRSV NSP1b). Cloned PRRSV NSP1, NSP1 alpha, and NSP1 beta is derived from PRRSV VR2332 (GenBank accession U87392) (Cheng Song et al, Virology, 2010). To identify differentially mRNAs in the PRRSV NSP1, PRRSV NSP1 alpha, PRRSV NSP1 beta transfectants and mock, three cDNA libraries from each transfectant group were generated and sequenced using the Illumina NovaSeq 6000 sequencer.