DRP004021
Identification of nitrogen-fixing Bradyrhizobium associated with roots of field-grown sorghum by metagenome analyses
Sorghum (Sorghum bicolor) grown worldwide. Although nitrogen fixation in sorghum has been studied since the 1970s, many of the N2-fixing bacteria in field-grown sorghum plants have not been identified because efforts were culture-dependent. The aim of this study was to identify functional N2-fixing bacteria associated with field-grown sorghum by omics approaches. To avoid contamination of plant DNA, we applied the combination of metagenome analyses based on extracted bacterial cells isolated from plant tissues. Sorghum plants were grown in a field in Fukushima, Japan, and bacterial cells was extracted from roots which was harvested at almost 100 days after transplanting.