PRJNA573070
Different peanut genotypes with oleic acid at four different stages
Four genotypes (AABB, aaBB, AAbb, and aabb) of eight F8 breeding lines were grown in the greenhouse at the temperature of 25 to 30 °C, and 60 to 90% relative humidity. Seed samples of each genotype from four seed developmental stages (yellow, orange, brown, and black) were flash frozen using liquid nitrogen and stored at -80? for RNA extraction. Total RNA were extracted using RNeasy Plant Mini Kit (Qiagen, CA, USA). The quality and quantity of total RNA were determined with gel electrophoresis and Nanodrop 2000 (Thermo Scientific). High quality RNA were used for transcriptome sequencing. Libraries were constructed and subsequently sequenced using Illumina HiSeq 4000 at the Beijing Genomics Institute (BGI).