GSE107762
Identification of PRMT5-dependent genes in ESA+CD24lowCD44+ MCF7 cells
PRMT5 has been associated with poor prognosis in breast cancer. Here, we identify genes which are dependent on PRMT5 expression in MCF7 ESA+CD24lowCD44+ breast cancer stem cells by RNA-seq Overall design: RNA-seq profiling of ESA+CD24lowCD44+ cells from MCF7 shCTRL or shPRMT5 cell lines. Three independent ESA+CD24lowCD44+ cell populations from MCF7 shCTRL or shPRMT5 cells were isolated by flow cytometry.