DRP005436
SCFAs supplementation exerts metabolic benefits
The 7-week-old C57BL/6J mice were fed on a HFD diet or HFD containing 5% cellulose, acetate, propionate, butyrate for 4 weeks in a factorial design. Feces were collected at after treatments. Fecal DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'.