ena-STUDY-HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ.-08-08-2017-12:52:16:805-1895
Protein-SIP experiment with a bacterial community enriched from a sulfidic and benzene-contaminated aquifer
A benzene-degrading, sulfate-reducing enrichment culture originating from a benzene-contaminated aquifer was investigated by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). The time-resolved, quantitative analysis of carbon fluxes within the community supplied with either 13C-labeled benzene or 13C-labeled carbonate yielded different functional groups of organisms, with their peptides showing specific time dependencies of 13C relative isotope abundance indicating different carbon utilization.
A benzene-degrading, sulfate-reducing enrichment culture originating from a benzene-contaminated aquifer was investigated by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). The time-resolved, quantitative analysis of carbon fluxes within the community supplied with either 13C-labeled benzene or 13C-labeled carbonate yielded different functional groups of organisms, with their peptides showing specific time dependencies of 13C relative isotope abundance indicating different carbon utilization.