16S rDNA cerrado soil
The first comprehensive survey of Brazilian Cerrado Soil Bacterial communities
For each vegetation type two soil areas were collected 50 meters apart and considered as replicates. DNA extraction was carried out, amplicons were generated for the V5 to V9 16S rDNA region, and each of the 8 amplicons samples was sequenced on an individual lane of a 454 Titanium sequencer.
The Brazilian Cerrado (a savanna-like vegetation) is the second largest biome in Brazil and is considered a biodiversity hotspot. This biome is composed by different vegetation types: Cerrado denso (trees in the range of 5 to 8 m), Cerrado sensu stricto (trees in the range of 3 to 6 m), Campo sujo (shrubs, sparse small trees and grasses) and Mata de galeria (gallery forest formation).