GSE185095
Transcriptome profiling of H3K27me3 demethylase deficient B cells
To assess how H3K27me3 demethylases, UTX and JMJD3, regulate B cell and plasma cell trasncriptomes. RNA-seq was performed on the following populations from control (CreCtrl) and double knockout (dKO) mice: 1) naïve marginal zone B (MZB) cells and follicular B (FOB) cells from control (CreCtrl) and double knockout (dKO) mice, 2) PC generated after three days of ex vivo culture of MZB or FOB cells, 3) PC generated at three days post in vivo stimulation with LPS Overall design: RNAseq on margnizal zone B cells, follicular B cells and plamsa cells, from CreCtrl and UTX- and JMJD3-deficient dKO mice