ena-STUDY-INSTITUTO DE BIOTECNOLOGIA UNIVERSIDAD NACIONAL DE-24-07-2018-16:05:49:762-880
Transcriptome analysis of the venom gland of Serradigitus gertschi by RNA-seq
The telsons of eight specimens of the Mexican scorpion Serradigitus gertschi were dissected, from which total RNA was extracted. A cDNA library was constructed that was further subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 17,363,454 reads were obtained, which assembled into 84,835 putative transcripts. From them, 21,410 where identified matching gene sequences in databases. A total of 119 putative transcripts with sequence similarity to venom components were identified.
The telsons of eight specimens of the Mexican scorpion Serradigitus gertschi were dissected, from which total RNA was extracted. A cDNA library was constructed that was further subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 17,363,454 reads were obtained, which assembled into 84,835 putative transcripts. From them, 21,410 where identified matching gene sequences in databases. A total of 119 putative transcripts with sequence similarity to venom components were identified.