GSE184560
Adaptation of Geobacter sulfurreducens to grow with fumarate only and its impact on the transcriptome
The expression profile of adapted G. sulfurreducens strain T9-1 grown with 40 mM fumarate without any other substrate versus the expression profile of G. sulfurreducens PCA (wild type) grown with 10 mM acetate and 40 mM fumarate Overall design: Illumina RNA-seq of total RNA extracted from G. sulfurreducens wild type grown anaerobically in NB medium with 10 mM acetate and 40 mM fumarate. Illumina RNA-seq of total RNA extracted from G. sulfurreducens strain T9-1 evolved by adaptive laboratory evolution to grow anaerobically in NB medium with only 40 mM fumarate. The adapted strain was transferred nine times in the fumarate-only medium and Total RNA was extracted from T9-1 performing fumarate disproportionation