ETEC_Carriage_in_Bangladesh-sc-4847
ETEC_Carriage_in_Bangladesh
O139 serogroup of Vibrio cholerae appeared in early 1990s and then disappeared by 2006. While it wa circulating, it dominated the surveillance charts but its sudden extinction surprised everyone. This study will look at the genomic changes that led to its success and decline over time. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Infection with the same gut pathogen results in symptomatic diarrhoeal disease in some individuals while others carry the pathogen asymptomatically. While this could be due to genetic differences at the host level, the role of microbiota and interaction of the pathogen with its microbiome in the gut cannot be ignored. In collaboration with icddr,b in Dhaka, Bangladesh we have extracted metagenomic DNA from the faeces of asymptomatic and symptomatic carriers of Enterotoxigenic E coli (ETEC). Asymptomatic and symptomatic ETEC infected subjects have been sampled and DNA from their faeces will be subjected to shotgun metagenomic sequencing to identify the difference in their gut microbiomes. This will help us establish symptomatic disease-microbiota association, if any. We expect the results to show clear diversity patterns linked to either asymtpmatic and symptomatic carriage of ETEC.