GSE106566
RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes after PTBP1 knockdown in human erythroid cells
Purpose: The goals of this study are to determine possible  downstream targets of PTBP1 on day8 of  erythroid differentiation with or without PTBP1 knockdown. Methods:gene expression profiling with or without PTBP1 knockdown in differentiated erythroblasts at day8  were generated by deep sequencing, induplicate, using Illumina GAIIx.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: heme metabolism genes were down-regulated after PTBP1 knockdown during erythroid differentiation Overall design: mRNA profiles of day8 erythroid cells with or without PTBP1knockdown were generated by deep sequencing, in duplicate, using Illumina GAIIx.