ena-STUDY-CNAG-26-03-2015-09:51:08:935-153
Exome sequencing of C3HeB/FeJ mouse lines after ENU mutagenesis
ENU mutagenesis in mice is applied in order to generate phenotypically interesting mouse lines that may serve as animal models for similar human phenotypes. Exome sequencing has become a popular method to evaluate these undirected mutagenesis experiments.  In this experiment, 8 mouse lines selected for their phenotypes after ENU mutagenesis were submitted to exome sequencing. The genetic background of these mice is C3HeB/FeJ, a suitable strain for ENU mutagenesis, which is relatively distant from the standard mouse reference genome. For pinpointing pehnotypically relevant mutagenesis-induced variants, sequences obtained from the mutant mouse lines were aligned against an alternative murine reference assembly, C3H/HeJ. Under this accession number, raw sequencing data in fastq format are made available for the 8 mutant strains used.
ENU mutagenesis in mice is applied in order to generate phenotypically interesting mouse lines that may serve as animal models for similar human phenotypes. Exome sequencing has become a popular method to evaluate these undirected mutagenesis experiments.  In this experiment, 8 mouse lines selected for their phenotypes after ENU mutagenesis were submitted to exome sequencing. The genetic background of these mice is C3HeB/FeJ, a suitable strain for ENU mutagenesis, which is relatively distant from the standard mouse reference genome. For pinpointing pehnotypically relevant mutagenesis-induced variants, sequences obtained from the mutant mouse lines were aligned against an alternative murine reference assembly, C3H/HeJ. Under this accession number, raw sequencing data in fastq format are made available for the 8 mutant strains used.