GSE175505
Allele-specific RNA-seq of single pollen precursors
Single pollen precursors were isolated from maize anthers, covering 3 weeks of development from before meiosis through mature pollen. Starting material was staged by anther length and microscopy, and anther stages were densely sampled throughout pollen development. All samples were from an F1 hybrid between A188 and B73 inbred lines, and transcripts were associated with a specific parental allele when possible. In the "transcript_counts.csv" file, each sample is given 4 columns to separate the allele calls: '_g1' contains counts matching the B73 allele, '_g2' contains counts matching the A188 allele, 'ua' means unassigned (no SNPs were present in the transcript), and '_cf' means conflicting (the transcript had SNPs matching both alleles -- this is most likely sequencing error). Code to reproduce all figures from the main text of Nelms and Walbot (2022) can be found at https://doi.org/10.5281/zenodo.6620997 Overall design: Allele-specific RNA-seq of single pollen precursors from maize anthers. Libraries were constructed with a modified version of CelSeq2.