ena-STUDY-Lorraine University-30-10-2018-16:19:39:960-501
Analysis of HIV-1 FTSJ3-dependent 2'-O-methylation
HIV-1 RNA was extracted from viral particles and analyzed by RiboMethSeq. Analysis was performed for WT HIV-1 RNA, corresponding in vitro transcripts, HIV-1 RNA produced in the cells with siRNA antiFTSJJ3  and anti-TRBP and for CRISPR/Cas9 deleted FTSJ3. Results show the existence of internal methylation sites in HIV-1 RNA, many of these sites are FTSJ3-dependent
HIV-1 RNA was extracted from viral particles and analyzed by RiboMethSeq. Analysis was performed for WT HIV-1 RNA, corresponding in vitro transcripts, HIV-1 RNA produced in the cells with siRNA antiFTSJJ3  and anti-TRBP and for CRISPR/Cas9 deleted FTSJ3. Results show the existence of internal methylation sites in HIV-1 RNA, many of these sites are FTSJ3-dependent