GSE98296
RNA-Seq Analysis of Spleen from Chicken and Japanese Quail infected with Lentogenic and Velogenic Newcastle Disease Virus
Newcastle disease (ND) affects a few hundred avian species including chicken, and the clinical outcome of Newcastle disease virus (NDV) infection ranges from mild to severe fatal disease depending on the NDV pathotype and the host species involved. Japanese quails serve as natural reservoirs of NDV and play important role in NDV epidemiology. While infection of chicken with velogenic NDV results in severe often fatal illness, the same infection in Japanese quails is results in in apparent infection. The molecular basis of this contrasting clinical outcomes of NDV infection is not yet known. We compared global gene expression in spleens of chicken and Japanese quails infected with a lentogenic or velogenic NDVs. We found contrasting regulation of key genes associated with NF-?B pathway and T-cell activation between chicken and Japanese quails. Our data suggests association of NDV resistance in Japanese quails to activation of NF-?B pathway and T cell proliferation. Overall design: RNA extracted from pools of spleens (n=6) from chickens or Japanese quail infected with Newcastle disease virus (LaSota or 2K3 strain) or mock-infected were analyzed by Illumina RNA-Seq and mapped to the Gallus gallus genome (Galgal4).