GSE193248
Liver colonisation by colorectal cancer metastases requires YAP-controlled plasticity at the micrometastatic stage
Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Successfully growing organoids display heterogeneous STAR expression and grow into big structures, while growth-compromised organoids stay small and are either entirely STAR-pos or STAR-neg. Overall design: Organoids were grown from single cells (APCKO/KO; KRASG12D/-; TP53KO/KO; SMAD4 KO/KO) for 12 days. Then, big and small organoids were size-separated from this culture, subcultures were dissociated to single cells, and STAR-high, STAR-mid, and STAR-neg cells were collected by FACS. Samples were processed for bulk RNA-seq.