PRJNA664583
Mapping regulatory enhancer RNAs and promoters with Low Quantity single strand CAGE (LQ-ssCAGE) method.
An experimental protocol for the Low Quantity (LQ) single strand (ss) CAGE "LQ-ssCAGE" method. The method enables cost-effective profiling of low quantity RNA samples especially those derived from cells cultured in small volumes, cellular compartments such as nuclear RNAs or for samples from developmental stages. High reproducibility and effectiveness of the LQ-ssCAGE method are demonstrated by constructing 240 libraries from 50 ng of THP-1 cell extracted RNAs and discovering lowly-expressed novel enhancer and promoter-derived lncRNAs.