E-MTAB-7260
RNA sequencing of Mus musculus T helper cell type 2 with retroviral overexpression of different genes
T cells were extracted from spleens of CBLA mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses overexpressing a gene of interest on day 1. On day 4, BFP-positive (infected) cells were FACS sorted and measured by a variant of SMART-seq2
T cells were extracted from spleens of CBLA mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses overexpressing a gene of interest on day 1. On day 4, BFP-positive (infected) cells were FACS sorted and measured by a variant of SMART-seq2