PRJNA520252
Viral DNA-capture-seq in HTLV-1 infected individuals
The retrovirus HTLV-1 inserts its viral genome into the host cellular DNA, achieves persistent infection, and induces human diseases, such as leukemia of the infected cells and chronic inflammation. Viral DNA-capture-seq is useful to characterize HTLV-1 provirus in naturally virus-infected individuals. The protocol provides precise and comprehensive information about the flanking host genome as well as the integrated viral sequence at single nucleotide resolution, which makes it possible to determine the proviral structure and the viral integration site.