PRJNA60763
Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Protocol Development - Clinical
The HMP early clinical pilot represents the dideoxy sequencing of 16S rRNA genes amplified from anonymized clinical stool samples, distributed to each of the four HMP sequencing centers. This pilot was conducted using a variety of pre-production versions of the sanger sequencing protocol to test primers, buffers, and PCR conditions.