GSE108349
Derivation of kidney organoids from human pluripotent stem cells [RNA-Seq: Data Set 1]
We have developed a robust methodology for the derivation of kidney organoids from human pluripotent stem cells further vascularized taking advantage of several in vivo approaches. Our methodology suffices for the generation of kidney derivatives transcriptomically comparable with 22 weeks of gestation kidney tissues and highlight the use of longer periods of exposure to 3D in order to promote renal differentiation in a time frame of 15 days. Overall design: mRNA profiles of undifferentiated and differentiatig cells were generated by deep sequencing, in duplicate, using Illumina system.