ciona
High-throughput sequence analysis of the SL trans-spliced mRNA population of tailbud embryos of the ascidian tunicate chordate Ciona intestinalis
The goal of the study was to globally identify the SL trans-spliced mRNAs of Ciona intestinalis tailbud embryos, and to map trans-splice acceptor sites onto the genome.  5'-segments of SL trans-spliced mRNAs were amplified by SL-PCR following random-primed reverse transcription.  SL-PCR products were sequenced using 454 technology on the Roche 454 GS 20 intrument.