GSE111944
Characterization of functional relationships of R-loops with gene transcription and epigenetic modifications in rice
We conducted genome-wide identification of R-loops followed by integrative analyses of R-loops with relation to gene expressionand epigenetic signatures in the rice genome. We found that the correlation between gene expression levels and profiled R-looppeak levels was dependent on the positions of R-loops within gene structures (hereafter named 'genic position'). Both antisenseonly (ASO)-R-loops and sense/antisense(S/AS)-R-loops sharply peaked around transcription start sites (TSSs), and these peaklevels corresponded positively with transcript levels of overlapping genes. In contrast, sense only (SO)-R-loops were generallyspread over the coding regions, and their peak levels corresponded inversely to transcript levels of overlapping genes. Inaddition, integrative analyses of R-loop data with existing RNA-seq, chromatin-immunoprecipitation (ChIP-seq), deoxyribonucleaseI-seq (DNase-seq) and whole-genome bisulfite sequencing (BS-seq) data revealed interrelationships and intricate connectionsamong R-loops, gene expression and epigenetic signatures. Experimental validation provided evidence that the demethylationof both DNA and histone marks can influence R-loop peak levels on a genome-wide scale. This is the first study in plants thatreveals novel functional aspects of R-loops, their interrelations with epigenetic methylation, and roles in transcriptionalregulation. Overall design: Examination of  two tissues in rice including seedlings and calli