ena-STUDY-AALBORG UNIVERSITY-21-05-2019-10:09:14:129-239
Enabling high-accuracy long-read amplicon sequences using unique molecular identifers and Nanopore sequencing
High-throughput amplicon sequencing of large genomic regions represents a challenge for existing short-read technologies. Long-read technologies can, in theory, sequence large genomic regions, but they currently suffer from high error rates. Here, we report a high-throughput amplicon sequencing approach that combines unique molecular identifiers (UMIs) with Oxford Nanopore sequencing to generate single-molecule consensus sequences of large genomic regions. We demonstrate the approach by generating nearly 10,000 full-length ribosomal RNA (rRNA) operons of roughly 4,400 bp in length from a mock microbial community consisting of 8 bacterial species using a single Oxford Nanopore MinION flowcell. The mean error rate of the consensus sequences was 0.05%, with no detectable chimeras due to a rigorous UMI-barcode filtering strategy. The simplicity and accessibility of this method paves way for widespread use of high-accuracy amplicon sequencing in a variety of genomic applications.
High-throughput amplicon sequencing of large genomic regions represents a challenge for existing short-read technologies. Long-read technologies can, in theory, sequence large genomic regions, but they currently suffer from high error rates. Here, we report a high-throughput amplicon sequencing approach that combines unique molecular identifiers (UMIs) with Oxford Nanopore sequencing to generate single-molecule consensus sequences of large genomic regions. We demonstrate the approach by generating nearly 10,000 full-length ribosomal RNA (rRNA) operons of roughly 4,400 bp in length from a mock microbial community consisting of 8 bacterial species using a single Oxford Nanopore MinION flowcell. The mean error rate of the consensus sequences was 0.05%, with no detectable chimeras due to a rigorous UMI-barcode filtering strategy. The simplicity and accessibility of this method paves way for widespread use of high-accuracy amplicon sequencing in a variety of genomic applications.