GSE168688
ChIP seq of mESC treated with doxorubicin
mESC were treated with doxorubicin and then histone modifications or p53 binding sites were analyzed Overall design: mESC were treated with doxorubicin 1uM or vehicle for 6 hours, washed out for 6 hours, then crosslinked with 1% formaldehyde at RT for 10 min, quenched with 0.125M glycine for 10 min, then snap frozen in liq N2. ChIP was performed as described in Rada-Iglesias, et al. Nature 2010.