GSE130361
Induction of human hemogenesis in adult fibroblasts by defined factors and hematopoietic co-culture
Previous work demonstrates an induction of definitive hematopoiesis from human dermal fibroblasts (HDFs) after transduction with the transcription factors (TFs) GATA2, GFI1B, and FOS. To improve the yield and function of the derived hematopoietic cells, TF screening identified GFI1 as a causative factor in hematopoietic cell expansion with in vitro and in vivo functional capacity. Overall design: mRNAseq profiling of adult human dermal fibroblasts (HDFs) at the population level after transduction with GATA2, GFI1, GFI1B and FOS at days 15 and 25