GSE116728
Contribution of SRF and Nkx2-5 to androgen-dependent gene expression in prostate cancer
To study and compare the effect of siRNA-mediated SRF or Nkx2-5 silencing on androgen-responsive genes in LNCaP cells. Overall design: Human LNCaP prostate cancer cells were transfected with siRNA SmartPools targeting SRF or Nkx2-5 or a non-targeting siRNA SmartPool.  Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle.  Three biological replicates were generated per treatment group.  Forty-eight hours later, total RNA was isolated and processed for RNA-Seq analysis.