d2de8141-1650-4e09-b194-381f75b3cef0
ARHGAP11B off-target integration analysis
We have previously generated two ARHGAP11A and ARHGAP11B double knockout iPSC clones by CRISPR/Cas9 and homology-directed repair. In this study, we would like to analyze, if these clones have any off-target integrations. For this purpose, we have performed whole genome sequencing of the two knockout clones and the respective source iPSC lines.
We have previously generated two ARHGAP11A and ARHGAP11B double knockout iPSC clones by CRISPR/Cas9 and homology-directed repair. In this study, we would like to analyze, if these clones have any off-target integrations. For this purpose, we have performed whole genome sequencing of the two knockout clones and the respective source iPSC lines.