GSE201984
Analysis of the Role of NuMA (TDIF) in DNA repair Through Next Generation Sequencing Techniques [NuMA (TDIF) and TDP1 CUT&RUN RPE1]
Purpose: To assess the role of NuMA (TDIF) in DNA repair  Methods: CUT&RUN was carried out in untreated/hydrogen peroxide treated RPE1 cells in duplicate. The data was mapped using bwa mem and subsequently used to generate bigwigs showing fold-change relative to inputs using MACS2 and various meta-feature profiles using the CGAT suite of bioinformatics tools  Results: The data revealed increased occupancy of NuMA (TDIF) at promoters and enhancers, which increases upon hydrogen peroxide treatment  Conclusions: Our study indicates that NuMA (TDIF) plays a role in the protection from and response to oxidative damage. This protective effect appears to be particularly strong at promoter and enhancer regions, and may differ between distinct subsets of gene categories. Overall design: Assessment of NuMA (TDIF) and TDP1 binding within the genome through CUT&RUN in RPE1 cells under untreated and hydrogen peroxide treated conditions in duplicate. MH = Untreated/H2O2-, 90R = H2O2 treated 90 minute recovery, I = IgG, N = NuMA (TDIF), T = TDP1