DRP006679
Restriction-site associated DNA sequencing of Nagoya and White Leghorn chickens and their F1 and F2 populations
By crosses between a male of Nagoya and two females of White Leghorn, an F1 population of 13 chickens were obtained. An F2 population of 252 chickens were produced from crosses between the F1 chickens obtained. Restriction-site associated DNA sequencing of genomic DNAs extracted from blood cells of all individuals was performed using two restriction enzymes of EcoRI and BglII to develop a high-density SNP linkage map in chickens.