GSE123777
The mithralog EC-7072 is highly cytotoxic to chronic lymphocytic leukemia cells by targeting the B-cell receptor signaling pathway
EC-7072, an analogue of Mithramycin A, exerts a direct cytotoxic effect on primary leukemic cells from patients with chronic lymphocytic leukemia (CLL) in vitro. To elucidate the underlying mechanisms mediating this effect, RNA sequencing was carried out employing total RNA from primary leukemic cells exposed to EC-7072. Data analysis revealed a dramatic impact of the compound on the transcriptional profile of CLL cells, unraveling a modulation of key mediators associated to homeostasis and survival of CLL cells, including B-cell receptor signaling. Likewise, EC-7072 activated the expression of a pro-apoptotic network, while downregulating the expression of essential anti-apoptotic proteins such as BCL2. Overall design: mRNA profiles of leukemic cells from 8 patients with chronic lymphocytic leukemia exposed to EC-7072