GSE171510
Pulmonary infection induces scarred lipidome influencing response to unrelated challenge
Resolution of infection results in development of trained innate immunity which is typically beneficial for the host in defense against unrelated secondary infection. Epigenetic changes underlie the establishment of trained innate immunity therefore host metabolism and this response are intimately linked. However, little is known regarding the influence of lipids on the development and function of trained immunity. Utilizing two models of pulmonary bacterial infection combined with multi-omic approaches, we identified persistent, pathogen-specific changes to the lung lipidome that correlated with differences in the trained immune response. Further, we establish the specific cellular populations in the lung that contribute to this altered lipidome. Together these results expand our understanding of the pulmonary trained innate immune response and the contributions of host lipids in informing that response. Overall design: Mice were inoculated intranasally with 1X10e5 CFU of Bordetella pertussis D420, 150 CFU of f. tularensis subspecies holarctica Live Vaccine Stratin (LVS), or PBS only. Whole lungs were harvested and digested into single cell suspensions for analysis using the 10X Genomics V.2 3' sequencing kit.