GSE162314
shRNA cassette sequencing from MycER MEF cells, treated with 2mM GLN or no GLN for ~35 days
Genome-wide shRNA-based screen to identify resistance genes during GLN starvation Overall design: 6x10^6 cells transduced with the mouse TRC2 library were starved of GLN for 4-5 weeks to screen for genes affecting survival and proliferation under GLN deprivation.