ena-STUDY-JIC-21-10-2019-09:42:50:656-72
Sequencing of purified isolates of Albugo laibachii with Illumina GAII
To quantify the level of diversity, and to identify genes undergoing adaptive selection, four isolates of Albugo laibachii were found in the field. The isolates were: Ash4 (Ashbrittle, Somerset, UK, collected with assistance of Nicola Perera), Abo1 (Ashbourne, Derbyshire, UK, collected by Eric Kemen and Ariane Kemen), Sua1 (Stratford upon Avon, Warwickshire, UK, collected by Ian Crute) and Went1 (Tu¨bingen, Baden Wu¨rttemberg, Germany collected by Eric Kemen). Isolates were purified by three rounds of single spore/single pustule propagation. DNA was prepared from conidiospores vacuumed from sporulating plants. Libraries were generated using Illumina TruSeq protocol and were sequenced on the Illumina GAII at The Sainsbury Laboratory. Additionally, a further lane of sequencing of isolate Em1 (first sequenced by Kemen et al, 2011 PLoS Biology) was performed to take advantage of advances in sequencing quality and efficiency.
To quantify the level of diversity, and to identify genes undergoing adaptive selection, four isolates of Albugo laibachii were found in the field. The isolates were: Ash4 (Ashbrittle, Somerset, UK, collected with assistance of Nicola Perera), Abo1 (Ashbourne, Derbyshire, UK, collected by Eric Kemen and Ariane Kemen), Sua1 (Stratford upon Avon, Warwickshire, UK, collected by Ian Crute) and Went1 (Tu¨bingen, Baden Wu¨rttemberg, Germany collected by Eric Kemen). Isolates were purified by three rounds of single spore/single pustule propagation. DNA was prepared from conidiospores vacuumed from sporulating plants. Libraries were generated using Illumina TruSeq protocol and were sequenced on the Illumina GAII at The Sainsbury Laboratory. Additionally, a further lane of sequencing of isolate Em1 (first sequenced by Kemen et al, 2011 PLoS Biology) was performed to take advantage of advances in sequencing quality and efficiency.