CRISPR_screening_to_interrogate_the_function_of_FAM50A_-sc-6557
CRISPR_screening_to_interrogate_the_function_of_FAM50A_
To interrogate the role of FAM50A in cancer cells we performed a genome wide CRISPR screen in a cell line with an inducible guide against FAM50A. Cells were treated +/- dox to allow comparison of hits between WT and FAM50A knockout cells.
A recent combinatorial screen within the Adams lab identified the paralogues FAM50A and FAM50B as potential synthetic lethal genes. These genes are poorly characterised with limited known function. To allow initial investigation into the function of these genes we have performed a genome wide CRISPR screen in a cancer cell line with and without FAM50A.