Analysis_of_bacteriophage_resistance_arising_among_a_population_of_Klebsiella_pneumoniae_after_phage_infection-sc-5177
Analysis_of_bacteriophage_resistance_arising_among_a_population_of_Klebsiella_pneumoniae_after_phage_infection
We observed on a number of occasions a number of colonies growing on a lawn of Klebsiella pneumoniae B5055 after bacteriophage infection. Characterisation of these phage resistant colonies would aid in identifying 'escape' routes for bacteria during phage infection. It would also allow us to complement and compare data we will generate in future upon infection of a TraDIS library of K. pneumoniae ATCC43816 mutants. We may expect point mutations as well as possible fragment loss or combination of both. Illumina Sequencing will thus help to pin down these DNA changes. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We observed on a number of occasions a number of colonies growing on a lawn of Klebsiella pneumoniae B5055 after bacteriophage infection. Characterisation of these phage resistant colonies would aid in identifying 'escape' routes for bacteria during phage infection. It would also allow us to complement and compare data we will generate in future upon infection of a TraDIS library of K. pneumoniae ATCC43816 mutants. We may expect point mutations as well as possible fragment loss or combination of both. Illumina Sequencing will thus help to pin down these DNA changes.