PRJNA488909
mutation site of Arabidopsis thaliana mutant crc1-1
F2 plants generated from backcrossing crc1-1 with Col-0 plants were inoculated with CMV-2aT?2b at the concentration of 50 µg/mL. Genomic DNA were extracted from about 100 susceptible F2 plants using CTAB reagent. DNA libraries were constructed according to standard Illumina protocols and used for SNP analysis by next-generation sequencing with illumina HiSeq2000 platform. The wild type genomic DNA was sequenced as a reference.