GSE129997
Re-configuration of Chromatin Structure During the Mitosis-G1 Phase Progression
Chromatin architecture is temporarily disrupted during mitosis. Yet, how chromatin architecture is reconfigured during M-G1 phase transition is unknown. To examine this, we performed Hi-C experiments on cell populations at defined cell cycle stages between mitosis and G1 phase. Overall design: Uninduced G1E-ER4 (-GATA1) cells were synchronized to pro-metaphase with nocodazole treatment and was released from nocodazole for a variety of durations. Cells at different cell cycle stages were purified with FACS. Hi-C experiments were performed on purified populations to examine chromatin structure at designated cell cycle stage.