PRJNA557435
RNA-seq of Danio rerio gonads exposed to elevated temperature during development
Zebrafish of the Nadia strain were raised at 28.5°C, 34°C, or 36°C from 18-30 days post-fertilization (dpf) to understand the developmental effects of high temperature on zebrafish that possess a genetic sex determinant. Fish were evaluated for genotypic and phenotypic sex and RNA-seq was performed on gonads collected at 90dpf. Strand-specific sequencing libraries were prepared with the BIOO NEXTflex Rapid Directional qRNA-Seq kit and sequenced on an Illumina HiSeq 4000.