PRJNA707266
L-DNA barcoding of microbial DNA extracted from the Lotus Pond at Tsinghua University, Beijing
Spiking information-storing L-DNA barcode into the microbial (D-) DNA extracted from the Lotus Pond was bioorthogonal in that it was specifically amplifiable by the mirror-image Pfu DNA polymerase with L-DNA primers, and did not affect the (D-DNA) metagenomic sequencing results.