ena-STUDY-IRB Barcelona-05-12-2014-11:25:36:889-246
Propagation of primary CRCs into organoids
We propagated in vitro primary CRCs as 3D primary cultures. CRC cells grew indefinitely as epithelial structures lacking stromal cells called tumor organoids. We expanded organoids from 8 primary CRCs. Exome sequencing analysis confirmed that these organoids displayed distinct combinations of mutations in the main driver pathways.
We propagated in vitro primary CRCs as 3D primary cultures. CRC cells grew indefinitely as epithelial structures lacking stromal cells called tumor organoids. We expanded organoids from 8 primary CRCs. Exome sequencing analysis confirmed that these organoids displayed distinct combinations of mutations in the main driver pathways.