GSE182843
sub-PEN-seq for identifying novel non-coding RNAs and their expression in cultured cells II
Small RNAs between 20-500nt were least explored. Here we used RNase H-based rRNA depletion and a serial of strategies to developed sub-PEN-seq method for identifying novel non-coding RNAs in Subcellular RNAs. Overall design: Subcellular RNA were ligated to  RNA adaptors, followed by rRNA and snRNA depletion. After reverse transcription and library amplification, inserts between 20-500bp were size selected and subjected to Illumina sequencing.