b2e7c267-8bf0-4c74-8e26-6c85535b3e88
De Novo Structural Elucidation of Enzymatically Produced Mannosides
Carbohydrates are complex structures that still challenge analysts today because of their different levels of isomerism, notably the anomerism of the glycosidic bond. We have used high-resolution IMS on a cyclic device to characterize the reaction products of Uhgb_MS, a novel mannoside synthase of the GH130 family. We performed IMSn analyses of enzymatically produced mannosides and,  we succeeded in determining the intrachain anomerism of a a1,2-mannotriose and a mix-linked ß/a1,2-mannotetraose. This is the first evidence that a single carbohydrate active enzyme can synthesize both a- and ß-glycosidic linkages.
Carbohydrates are complex structures that still challenge analysts today because of their different levels of isomerism, notably the anomerism of the glycosidic bond. We have used high-resolution IMS on a cyclic device to characterize the reaction products of Uhgb_MS, a novel mannoside synthase of the GH130 family. We performed IMSn analyses of enzymatically produced mannosides and,  we succeeded in determining the intrachain anomerism of a a1,2-mannotriose and a mix-linked ß/a1,2-mannotetraose. This is the first evidence that a single carbohydrate active enzyme can synthesize both a- and ß-glycosidic linkages.