E-MTAB-10688
Analysis of HIF dependent transcriptional regulation resolved by transcriptional start site, using 5' end-Seq technology
Changes in mRNA abundance upon HIF activation and/or hypoxia in VHL-defective kidney cancer cell were analysed. Sequencing of the 5' ends of mRNAs (5' end-Seq) was applied to VHL-defective kidney cancer cell lines with or without reintroduction of VHL and/or with hypoxic incubation (1% O2 for 24h) to analyse the abundance of mRNAs resolved by their transcription start sites. In addition, the contribution of HIF pathway was confirmed using 786-O cells in which HIF1B was inactivated.
Changes in mRNA abundance upon HIF activation and/or hypoxia in VHL-defective kidney cancer cell were analysed. Sequencing of the 5' ends of mRNAs (5' end-Seq) was applied to VHL-defective kidney cancer cell lines with or without reintroduction of VHL and/or with hypoxic incubation (1% O2 for 24h) to analyse the abundance of mRNAs resolved by their transcription start sites. In addition, the contribution of HIF pathway was confirmed using 786-O cells in which HIF1B was inactivated.