ena-STUDY-Jeju National Univeristy-18-01-2017-02:30:43:560-258
Phreatobacter yongcheonensis sp. nov., isolated from lava tube and emended description of the genus Phreatobacter
An aerobic, Gram-negative, catalase-positive, oxidase-positive bacterium, designated strain YC6-17T, was isolated from pieces of wood collected at the bottom of Youngcheon Cave in Jeju, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YC6-17T formed a tight cluster with Phreatobacter oligotrophus DSM 25521T (98.07% sequence similarity) and distinctly separated from other taxa of the order Rhizobiales. Cells were motile rods and showed growth at 10-30 °C, pH 6-8 and 2% NaCl. Colonies were smooth, circular, convex and pale yellow in colour. The predominant ubiquinone was Q-10. The predominant fatty acids were C18:1?7c, C16:0 and C18:0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unknown glycolipids and an unknown lipid. The DNA G+C content was 64.4mol%. DNA-DNA relatedness between strain YC6-17T and the closest relative was 15.6±7.9%. Based on the combined data from phenotypic characterization and DNA-DNA hybridization as well as phylogenetic analysis, it was suggested that the isolate represents a novel species of the genus Phreatobacter, for which Phreatobacter yongcheonensis sp. nov. sp. nov. is proposed. The type strain is strain YC6-17T (= KCTC 52518T = DSM 103734T).
An aerobic, Gram-negative, catalase-positive, oxidase-positive bacterium, designated strain YC6-17T, was isolated from pieces of wood collected at the bottom of Youngcheon Cave in Jeju, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YC6-17T formed a tight cluster with Phreatobacter oligotrophus DSM 25521T (98.07% sequence similarity) and distinctly separated from other taxa of the order Rhizobiales. Cells were motile rods and showed growth at 10-30 °C, pH 6-8 and 2% NaCl. Colonies were smooth, circular, convex and pale yellow in colour. The predominant ubiquinone was Q-10. The predominant fatty acids were C18:1?7c, C16:0 and C18:0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unknown glycolipids and an unknown lipid. The DNA G+C content was 64.4mol%. DNA-DNA relatedness between strain YC6-17T and the closest relative was 15.6±7.9%. Based on the combined data from phenotypic characterization and DNA-DNA hybridization as well as phylogenetic analysis, it was suggested that the isolate represents a novel species of the genus Phreatobacter, for which Phreatobacter yongcheonensis sp. nov. sp. nov. is proposed. The type strain is strain YC6-17T (= KCTC 52518T = DSM 103734T).