STUDY_THE_EFFECT_OF_TGF_beta_ON_SCHISTOSOME_ADULT_WORMS-sc-2013-07-15T13:12:29Z-340
STUDY_THE_EFFECT_OF_TGF_beta_ON_SCHISTOSOME_ADULT_WORMS
STUDY THE EFFECT OF TGF beta ON SCHISTOSOME ADULT WORMS    Goal: Primary: Assess the effects of TGF beta on transcription of adult male and female schistosomes.             Secondary: Identify the pathways regulated by TGF beta in worm pairs, mature males, mature females, immature males and immature females.      METHODS:  Parasite: Schistosoma mansoni employed is the NMRI strain that is maintained in a Puerto Rican Biomphalaria glabrata and Golden Syrian hamsters. The life cycle is maintained in the LoVerde lab.    Cercariae obtained from infected snails were used to infect experimental animals. The cercariae wre shed from snails exposed to multiple miracidia for mixed infections or from snails exposed to a single miracidia for single-sex infections. Schistosome worms were recovered from hamsters by perfusion (Duvall and DeWitt, 1967) 45 days post infection.      Freshly perfused adult worms containing mixed-sex or single-sex infections were collected from infected golden hamsters, washed and incubated for overnight at 37Â°C, 5% CO2 in MEM medium supplemented with 1 mM sodium pyruvate, 1 X non-essential amino acid solution, 1 X amino acid solution, 100 U ml penicillin, 100 ug ml streptomycin 1 ug ml amphotericin B (antibiotic antimycotic solution) and 2 mM Glutamax-I. Recombinant human TGF- beta1 was added individually to about 25 pairs or 30 individual schistosomes to a final concentrations of 1.0 nM and incubation was continued for another 24 hr. The controls were treated as above except no TGF-beta1 was added. The worms were placed in RNAlater and sent to WTSI (Anna) for RNA extraction and sequencing.    Duvall RH, Dewitt WD. An improved perfusion technique for recovering adult schistosomes from laboratory animals. Am J Trop Med Hyg 1967;16:483-486.  . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
transcriptome of adult worms treated with TGF-beta in different conditions and the corresponding controls