GSE131124
iCLIP analysis of SRSF4 binding profile in neonatal cardiomyocytes
The goal of this study is to analyse binding preferences of SRSF4 in cardiac myocytes. Overall design: Neonatal cardiomyocytes were extracted from C57BL/6 mice at P0 or P1.23 A total of 8 million cells were plated in 10 cm2 plates. The following day, cells were transfected with 15 µg of SRSF4-GFP modRNA using 21 µL Lipofectamine 2000. At 48 h post-transfection, cells were irradiated once with 100 mJ UV light (254 nm), and iCLIP was performed