ena-STUDY-QUEEN'S UNIVERSITY BELFAST-23-01-2020-12:25:48:086-92
Manure microbiome of dairy cows fed grass or grass silage diets
Manure samples were taken from 18 dairy cows on one farm.  Cows were Holstein-Friesian or Holstein-Friesian cross and all from the same herd but kept under two different systems.  Nine animals were from a group kept at pasture with a diet of grazed grass plus 5.5-7kg of concentrated per day.  The other nine were from a group kept in a cubicle house with a diet of ad libitum grass silage plus 10kg concentrates per day.  Samples were taken directly after defecation and transported to the laboratory on ice.  They were frozen at -20C until DNA extraction using Qiagen Powersoil Pro kit (according to manufacturer's instructions).  The v4 region of the 16s rRNA gene was sequenced on an Illumina Miseq (250bp paired end reads).
Manure samples were taken from 18 dairy cows on one farm.  Cows were Holstein-Friesian or Holstein-Friesian cross and all from the same herd but kept under two different systems.  Nine animals were from a group kept at pasture with a diet of grazed grass plus 5.5-7kg of concentrated per day.  The other nine were from a group kept in a cubicle house with a diet of ad libitum grass silage plus 10kg concentrates per day.  Samples were taken directly after defecation and transported to the laboratory on ice.  They were frozen at -20C until DNA extraction using Qiagen Powersoil Pro kit (according to manufacturer's instructions).  The v4 region of the 16s rRNA gene was sequenced on an Illumina Miseq (250bp paired end reads).