GSE168965
Defining the transcriptional regulon of ChvI in Caulobacter crescentus
We sought to define the transcriptional regulon of the ChvI response regulator in Caulobacter crescentus. This study compares gene expression between ?chvI cells and cells overexpressing the constitutively-active chvI(D52E) mutant. Our work provides a global view of the genes directly and indirectly regulated by the ChvGI two-component system in C. crescentus. Overall design: 6 replicates of Caulobacter cresentus ?chvI EV and ?chvI chvI(D52E)++ cultures were grown in PYE + 0.15% xylose before total RNA extraction using TRIzol. RNA-seq reads were mapped to the NA1000 reference genome (CP001340).