GSE162430
D-xylose catabolic pathways expressed in Herbaspirillum seropedicae
Herbaspirillum seropedicae are ß-proteobacteria that establish as endophytes in various plants. They are able to consume diverse carbon sources, including hexoses and pentoses like D-xylose. D-xylose catabolism pathways have been described in some microorganisms, but databases of genes involved in these routes are limited. This is of special interest in biotechnology, considering that D-xylose is the second most abundant sugar in nature. Furthermore, it is found in some potential raw materials such as lignocellulosic biomass. In this work we present a study of D-xylose catabolism pathways in H. seropedicae strain Z69, using RNA-seq analysis and the subsequent study of phenotypes determined in targeted mutants in corresponding identified genes. G5B88_22805 gene, designated xylB, encodes a NAD+- dependent D-xylose dehydrogenase. Mutant Z69?xylB was still able to grow on D-xylose, although at a reduced rate. This is due to expression of an L-arabinose dehydrogenase encoded by G5B88_05250 gene, and was thus able to use D-xylose as substrate. According to our results, H. seropedicae Z69 uses non-phosphorylative pathways to catabolize D-xylose. The lower portion of metabolism involves co-expression of two routes: Weimberg pathway that produces a-ketoglutarate and a novel pathway recently described that produces pyruvate and glycolate. This novel pathway seems to be essential since a mutant in the last step of this pathway, Z69?G5B88_06410, was unable to grow on D-xylose. Overall design: mRNA profiles of 3 samples: Herbaspirillum seropedicae Z69 grown until mid-log phase in LGI medium supplemented with xylose 30g/l or glycerol 10g/l and a knock-out mutant strain in xylB gene (G5B88_22805) grown until mid-log phase in LGI medium supplemented with xylose 30g/l. The experiment was done in duplicate (biological replicates). Z69 grown on glycerol was used as control condition.