Resequencing_of_a_2_7Mb_region_of_chromosome_5_of_a_deaf_mouse_mutant_strain_-sc-126
Resequencing_of_a_2_7Mb_region_of_chromosome_5_of_a_deaf_mouse_mutant_strain_
Illumina paired-end sequencing of genomic DNA from a 2.7Mb region of mouse chromosome 5, using DNA from two mice from a deaf mutant strain. Libraries were made from mouse genomic DNA that had been hybridised to a pulldown microarray designed against the region (Nimblegen). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Deafness is the most common sensory deficit in the human population, but the genetic basis for nonsyndromic hearing loss is still largely unknown. Deaf mouse mutants are a powerful tool for discovering genes which affect hearing and characterising the pathways which, when perturbed, result in dysfunction of the ear.  Bronx waltzer is a spontaneous autosomal recessive mutant which has been previously described (Deol and Gluecksohn-Waelsch, 1979). Homozygotes demonstrate rapid degeneration of vestibular hair cells and cochlear inner hair cells at a late embryonic stage, resulting in deafness and vestibular dysfunction, but outer hair cells remain intact (Whitlon et al, 1996, Cheong and Steel, 2002). The mutation has been mapped to a 2.7Mb region on chromosome 5, but exon resequencing has failed to find a potential causative mutation (Bussoli et al, 1997, Taylor, 2005). We have carried out Illumina paired-end sequencing of the entire region, both coding and non-coding sequences, in order to locate candidates.