E-MTAB-2950
The first wave of the zygotic transcription is highly promiscuous and uncoupled from splicing and 3 prime end processing.
Initiation of zygotic transcription in mammals is poorly understood. In mice, zygotic transcription is first detected shortly after pronucleus formation in 1-cell embryos but the identity of the transcribed loci and mechanisms regulating their expression are not known. Using total RNA-seq we find that transcription in 1-cell embryos is highly promiscuous such that intergenic regions are extensively expressed and thousands of genes are transcribed at relatively low levels.
Initiation of zygotic transcription in mammals is poorly understood. In mice, zygotic transcription is first detected shortly after pronucleus formation in 1-cell embryos but the identity of the transcribed loci and mechanisms regulating their expression are not known. Using total RNA-seq we find that transcription in 1-cell embryos is highly promiscuous such that intergenic regions are extensively expressed and thousands of genes are transcribed at relatively low levels.