GSE130363
Transcriptional changes in the breast cancer cell line MCF7 rendered resistant to the cationic drug siramesine
Repurpusing cationic amphiphilic drugs (CADs) may be enhance the effect of chemotherapy in cancer treatment. In order to understand the molecular mechanism, we created cell lines resistant to the CAD siramesine by growing them for 6 months in increasing concentrations of siramesine. Parental and resistant cell lines were subjected to RNAseq and revealed diffentially expressed genes in ca+ and cAMP signalling pathways. Overall design: Parental and CAD resistand cell lines were profiled by RNAsequencing in dublicate and triplicate using the Illumina 2000 plarform