ena-STUDY-TEXAS A AND M UNIVERSITY, Kingsville-05-03-2019-02:41:05:144-102
Seq [organism=Pectobacterium carotovorum subsp. carotovorum] [clone=cel12B] gene, Complete cds
The bacterium Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) is notorious for its maceration of the plant cell wall causing soft rot. The ability to destroy plants is due to the expression and secretion of a wide range of hydrolytic enzymes that include cellulases and polygalacturonases. P. carotovorum ATCC™ no. 15359 was used as a source of DNA for the amplification of cel12B, a gene encodes  for a cellulase. Primers were designed based on published gene sequences and used to amplify the open reading frames from the genomic DNA of P. carotovorum. The individual PCR products were cloned into the pTAC-MAT-2 expression vector and transformed into Escherichia coli. Molecular and biochemical studies were conducted for the assimilated clone product alongside three other clones' isolated using the same source, which could provide evidence for successful transformation of a lignocellulosic biomass oligomers into biofuel related precursors. *the clone's DNA was sequenced via Molecular Cloning Laboratories ex-facility (MCLAB, San Francisco, CA, USA).>Seq ATGCTTACAGTGAATAAGAAGCCACACCGTATCTTCCACACATTATTTCCCATATTATTTTCTGCCTTGTTACTCTCCCCATTCACCGCTTCTGCGGTCAGTTCCTCCAAAGACGCCGACAAACTCTATTTTGAGAATAATAAATATTATGTATTCAATAACGTCTGGGGAAAAGATGAAGTAAAAGGATGGCAGCAAACGGTTTTCTATAACAGCCCAACCAGCATGGGGTGGAACTGGCACTGGCCAAGTAACTCCTCCAGCGTTAAAGCCTATCCATCACTGGTGAGCGGTTGGCACTGGACGGCGGGCTATACGGCAAATAGCGGCTTGCCGATAAAATTGTCCAGTAATAAAAGTATTACCAGTAACGTCACCTATTCCATCAAATCCACTGGCACTTACAACGCGGCTTATGACATTTGGTTCCACACCACCGACAAAGCCAGTTGGGATTCTACCCCTACCGATGAATTAATGATCTGGCTAAATAATACCAATGCAGGCCCGGCGGGTGATTATATTGAAACAGTTTTCCTCGGTGGCAGCAGTTGGAACGTATTCAAAGGCTGGATCAACGCCGGTAATGGCAAAGGGTGGAATGTCTTTTCCTTTGTTCGTACCTCCAATACCAACAACGCATCGCTCAATATTCGCCATTTCACCAACTATCTGGTGGGAACCAAGAAATGGATGAGCAATACAAAATATATCAGCAGCGTTGAGTTTGGCACCGAGATCTTTGGCGGTGATGGGCAGATTGATATCACCAAGTGGAGCGTAGACGTAAAATAA
The bacterium Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) is notorious for its maceration of the plant cell wall causing soft rot. The ability to destroy plants is due to the expression and secretion of a wide range of hydrolytic enzymes that include cellulases and polygalacturonases. P. carotovorum ATCC™ no. 15359 was used as a source of DNA for the amplification of cel12B, a gene encodes  for a cellulase. Primers were designed based on published gene sequences and used to amplify the open reading frames from the genomic DNA of P. carotovorum. The individual PCR products were cloned into the pTAC-MAT-2 expression vector and transformed into Escherichia coli. Molecular and biochemical studies were conducted for the assimilated clone product alongside three other clones' isolated using the same source, which could provide evidence for successful transformation of a lignocellulosic biomass oligomers into biofuel related precursors. *the clone's DNA was sequenced via Molecular Cloning Laboratories ex-facility (MCLAB, San Francisco, CA, USA).>Seq ATGCTTACAGTGAATAAGAAGCCACACCGTATCTTCCACACATTATTTCCCATATTATTTTCTGCCTTGTTACTCTCCCCATTCACCGCTTCTGCGGTCAGTTCCTCCAAAGACGCCGACAAACTCTATTTTGAGAATAATAAATATTATGTATTCAATAACGTCTGGGGAAAAGATGAAGTAAAAGGATGGCAGCAAACGGTTTTCTATAACAGCCCAACCAGCATGGGGTGGAACTGGCACTGGCCAAGTAACTCCTCCAGCGTTAAAGCCTATCCATCACTGGTGAGCGGTTGGCACTGGACGGCGGGCTATACGGCAAATAGCGGCTTGCCGATAAAATTGTCCAGTAATAAAAGTATTACCAGTAACGTCACCTATTCCATCAAATCCACTGGCACTTACAACGCGGCTTATGACATTTGGTTCCACACCACCGACAAAGCCAGTTGGGATTCTACCCCTACCGATGAATTAATGATCTGGCTAAATAATACCAATGCAGGCCCGGCGGGTGATTATATTGAAACAGTTTTCCTCGGTGGCAGCAGTTGGAACGTATTCAAAGGCTGGATCAACGCCGGTAATGGCAAAGGGTGGAATGTCTTTTCCTTTGTTCGTACCTCCAATACCAACAACGCATCGCTCAATATTCGCCATTTCACCAACTATCTGGTGGGAACCAAGAAATGGATGAGCAATACAAAATATATCAGCAGCGTTGAGTTTGGCACCGAGATCTTTGGCGGTGATGGGCAGATTGATATCACCAAGTGGAGCGTAGACGTAAAATAA