PRJNA563043
Arabidopsis thaliana strain:Columbia-0 Raw sequence reads
Total RNA was extracted from inflorescence tissues of different genotypes. Sequencing libraries were constructed using KAPA mRNA HyperPrep kit with a modified protocol. In brief, mRNA was captured by magnetic oligo-dT beads (Kapa Biosystems) and fragmented into short sizes by incubating at 94 °C for 15min (KAPA mRNA HyperPrep Kit, Cat. KR1352). After second strand synthesis and A-tailing, cDNA fragments larger than 150 bp were discarded by right side selection (i.e. larger fragments bound to beads are discarded and smaller fragments in the supernatant are kept.) using 1x volume of Ampure beads (Beckman Coulter). Two additional right side selections using 1.8x volume of Ampure beads were conducted after adapter ligation and library amplification, respectively. Library DNAs ranging from 160 to 220 bp (corresponding to insert size 40~100 bp) were recovered from polyacrylamide gel and use the illumina Hiseq X-Ten platform for sequencing (Genenergy Inc. Shanghai, China).