GSE138994
A genome-wide approach to delineate novel MLL4 targets involved in skeletal muscle metabolism
This experiment was conducted to identify novel MLL4 targets in skeletal muscle Enhancers play central role in controlling gene expression in time and space. Primed enhancers are marked by histone H3 lysine 4 (H3K4) mono/di-methylation (H3K4me1/2). Mixed-lineage leukemia 4 (MLL4/KMT2D) is an evolutionarily conserved H3K4me1/2 methyltransferase that is required for enhancer activation. Here, we identified the genome-wide MLL4 occupancy in mouse skeletal muscle by ChIP-seq coupled with RNA-seq analysis. Gene ontology analysis revealed that MLL4 controls muscle fiber-type switching. We thus have revealed novel MLL4 targets involved in muscle metabolism. Overall design: Using genome-wide approach, ChIPseq,  to delineate novel MLL4 targets involved in skeletal muscle metabolism