GSE184665
Global mapping of the macrophage-HIV-1 transcriptome reveals that productive infection induces remodeling of the host cell DNA and chromatin [miRNA-seq]
This data corresponds to the microRNA expression profiles for the exact same samples from GSE180273. Overall design: Human monocytes from healthy donors were differentiated in MØ by adherence for 3 days in the presence of RPMI-1640 culture medium supplemented with M-CSF (25 ng/mL) and 10% human AB serum. Cells were maintained in culture medium in absence of M-CSF for an additional 3 days, treated with Accutase™ (Stem Cell Technologies) for 60 to 90 min, and detached with a soft cell scraper (Sarstedt). Cells were plated at 2.5 x 10exp5 cells/mL for 24 (cell-culture coated dishes) or 72 h (Ultra-Low attachment dishes). MØ were infected with replication-competent NL/Bal-HSA (see A. Deshiere et al., Scientific Reports 2017) with multiplicities of infection (MOI) ranging from 0.004 to 2.5. HSApos and HSAneg MØ were isolated using biotinylated anti-HSA (clone M1/69; eBiosciences) and anti-Biotin Magnetic Beads (Miltenyi) in a MS separation column (Miltenyi). Purified MØ populations (HSAneg and HSApos) were resuspended and frozen in QIAzol lysis buffer. Total RNA was isolated from sorted cell populations using the miRNeasy Kit from QIAGEN (Valencia, CA).