GSE173494
Enhanced lipogenesis through Ppar? helps cavefish adapt to food scarcity
To test wheather cavefish have higher lipogenesis capability than surface fish, we used liver sample from fasted and refed fish to do RNA-seq to compare their transcriptome responding to feeding in surface fish and Pachón cavefish. Moreover, we did the Ppar? ChIP-seq with liver from fed surface fish and Pachón cavefish. We amied to test whether Ppar? in Pachón cavefish have more biding sites and higher binding peaks than surface fish. Overall design: For RNA-seq study, we 2 feeding regimes(starved and refed) and 2 fish populations (surface and Pachón cavefish). We had 3 replicates for each fish group. For ChIP-seq, we had one input control and two replicates for each fish population (surface and Pachón cavefish).