GSE136817
PPARa - let-7 microRNA network in hepatic lipid metabolism [ChIP-seq]
Our quantitative RT-PCR analysis suggested that PPARa activation repressed let-7 miRNA expression in mouse liver. The mechanisms by which PPARa suppressed gene expression remains largely unknown. To determine the mechanism of target gene suppression, PPARa binding sites in the mouse liver chromatin was analyzed by ChIP-seq after PPARa agonist treatment. Overall design: Vehicle (Control) or PPARa agonist (Wy-14,643) treated mouse livers were removed from mice and subjected to Chromatin immunoprecipitation by Control (IgG) or PPARA antibody (PPARA). The purified ChIPed DNA was subjected to deep sequencing.