GSE134262
Natural killer cells suppress T cell tumor immune evasion [B16-F10 CRISPR/Cas9 NK cell screen]
Investigation of tumor genes which limit sensitivity to killing by NK cells through a genome-wide in vitro CRISPR/Cas9 screen Overall design: B16-F10 Cas9 expressing cells transduced with a genome-wide CRISPR library were successively co-cultured overnight with activated NK cells at an equal ratio. Experiment was performed in technical duplicate. An untreated control cultured alongside was used for comparison