GSE125855
Mapping polymerase usage by tracking ribonucleotide incorporation (HydEn-seq) during DNA replication
We devised and improved on our hydrolytic end sequencing (HydEn-seq) that mapping ribonucleotide incorporation in genome and used this method to track DNA replicative polymerase usage. We uncovered striking exceptions to canonical polymerase division of labor in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Overall design: Examination of location of ribonucleotides in multiple yeast strains bearing different DNA polymerase variants, deficient in rnh201.