GSE130477
Examination of ATF1 transcription factor binding peaks in colorectal cancer HCT116 cells
ATF1 encodes a sequence-specific activating TF containing a bZIP DNA binding domain, which is overexpressed in CRC tumor tissues compared with their paired normal tissues in two independent cohorts and that ATF1 amplification also frequently occurs across multiple cancer types. Moreover, ATF1 is more highly expressed in advanced stages of CRC. Mechanistically, ATF1 overexpression could provoke cell proliferation and xenograft growth in vitro and in vivo by affecting cell apoptosis, However, the exact mechanism and downstream transcriptional programs by which ATF1 provokes tumor activity are not well understood. We performed ChIP-seq in HCT116cells to investigate the regulatory mechamisms of ATF1. Overall design: HCT116 cells treated with 3xFlag-ATF1 and then performed a falg-ATF1_ChIP-Seq.