PRJNA725093
Oncogenic patterns and cancer predisposition through the trascriptomic profile in a Mitchell Riley syndrome with heterotopic gastric mucosa and duodenal atresia
Homozygous mutations in the transcription factor RFX6 are the cause of the Mitchell-Riley syndrome (MRS) associating neonatal diabetes, congenital digestive system, such as biliary atresia, pancreatic hypoplasia, duodenal and or jejunal atresia, intestinal malrotation, gallbladder aplasia, cholestasis. A constitutive inactivation of RFX6 leads also to gastric heterotopia. Application of RNAseq in human diseases may help to better understand pathogenic mechanism of diseases and to predict the risk of developing chronic disorders and personalizing their prevention and treatment. We evaluated oncogenic patterns and cancer predisposition through the trascriptomic profile in a MRS with neonatal diabetes, duodenal atresia and heterotopic gastric mucosa extensive. We signalled the interactors of RFX6 with other up and downregulated genes, that may be interested in severity of diabetic condition, in multi-organs impairment and cancer predisposition. Furthermore, several dysregulated genes are involved in biological processes that can lead to promote cancer including Evading apoptosis (BAD, BBC3, EGF, FGFR2, FLT3LG, HMOX1, HRAS, IFNAR2, IGF1R, IL12RB1, IL13RA1, IL15, IL2RB, IL2RG, IL6R, KEAP1, MGST1, PDGFA, PDGFRB, PIK3R3, RALB, RALGDS, RASSF1, SOS1, TGFA, TXNRD3), Proliferation (APC, BRAF, CCND2, CCND3, CCNE2, FGFR2, FLT3LG, FZD1, FZD6, HMOX1, HRAS, IGF1R, KEAP1, LRP6, MAPK3, MGST1, PDGFA, PDGFB, PDGFRB, RB1, SOS1, TGFA, TXNRD3, WNT10B), Sustained angiogenesis (BRAF, FGFR2, FLT3LG, HRAS, IGF1R, JAG1, MAPK3, NOTCH2, PDGFA, PDGFB, PDGFRB, SOS1, TGFA, TGFB1), Genomic instability (BAD, BBC3) and Insensitivity to anti-growth signals (SMAD2, TGFB1). We also inspected the signalings and their related genes in cancer, such as PI3K signaling, ERK signaling, JAK-STAT signaling , Calcium signaling, Other RAS signaling, WNT signaling. In our MRS patient we signalled the interactors of RFX6 with other up and downregulated genes, that may be interested in severity of diabetic condition, in multi-organs impairment and cancer predisposition. Notably, a lot of dysregulated genes are able to trigger carcinogenesis. The possibility for the patient to develop cancer degeneration in heterotopic gastric mucosa and/or additional long term tumoral sequelae is not excluded. A personalized prevention and treatment strategies should be proposed.