ena-STUDY-ENS Lyon-13-03-2017-10:42:36:570-325
Mapping-by-sequencing data for mutant allele mf53 (Oscheius tipulae)
The Oscheius tipulae strain JU71 carries the recessive mutation mf53 in the CEW1 background (reference genome). mf53 is an allele of the locus cov-1, published in Louvet-Vallee S. et al., 2003 (PMID:12586702)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 128 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.
The Oscheius tipulae strain JU71 carries the recessive mutation mf53 in the CEW1 background (reference genome). mf53 is an allele of the locus cov-1, published in Louvet-Vallee S. et al., 2003 (PMID:12586702)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 128 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.