GSE158713
Gene expression profiling using RNA-Seq data of MMTV-Wnt1 tumor tissue from mice treated with E7386
We conducted an RNA-Seq analysis using total RNA extracted from MMTV-Wnt1 tumor tissues resected from mice orally administered E7386 at 25 or 50 mg/kg twice daily, or vehicle, for 3 days (Day 4) or 7 days (Day 8) and examined the differentially expressed gene levels compared with vehicle at days 4 and 8. Overall design: MMTV-Wnt1 transgenic mice (B6SJL-Tg(Wnt1)1Hev/J) were obtained from the Jackson Laboratory (Bar Harbor, ME). Spontaneously developed breast tumors were resected from the MMTV-Wnt1 transgenic mice, diced, and inoculated into C57BL/6J mice (Charles River Laboratories Japan). Tumors were allowed to grow to approximately 200 mm3 in size (designated Day 1); tumor-bearing mice were then randomly allocated to treatment groups and orally administered E7386 (25.0, or 50.0 mg/kg twice daily) for 3 days (Day 4) or 7 days (Day 8).