ena-PROJECT-carbonara-01-04-2012-20:39:52:024-1
Isolation of a new lysine producing C. glutamicum mutant
The wild type of C. glutamicum carrying the L-lysine sensor was treated with mutagen. Out of 6.5x106 cells 270 were selected using FACS within 30 minutes, of which 225 accumulated 3-38 mM L-lysine. Targeted sequencing identified 13 new chromosomal mutations in the known targets lysC and hom. From 10 mutants with no mutation in known targets the entire genome was sequenced using Illumina HiSeq 2000 technology. In strain K051 a murE mutation was identified which when introduced into existing L-lysine producers improved the L-lysine titers significantly.
The wild type of C. glutamicum carrying the L-lysine sensor was treated with mutagen. Out of 6.5x106 cells 270 were selected using FACS within 30 minutes, of which 225 accumulated 3-38 mM L-lysine. Targeted sequencing identified 13 new chromosomal mutations in the known targets lysC and hom. From 10 mutants with no mutation in known targets the entire genome was sequenced using Illumina HiSeq 2000 technology. In strain K051 a murE mutation was identified which when introduced into existing L-lysine producers improved the L-lysine titers significantly.