PRJNA732384
Phosphothreonine Dependent High Throughput Interactome of Phosphoproteins
Using phosphothreonine as a non-standard amino acid, we characterize the interactions of the phospho-binding domain, 14-3-3 beta with the human phosphothreonine proteome utilizing HI-P. Using HI-P+ we determine the multi-level binding interactions of 14-3-3 beta with CHK2 kinase-dependent substrates.