GSE129384
Analysis of gene expression in colonic epithelial cells following DSS treatment
Mice with and without IFNAR expression were treated with broad-spectrum antibiotics for more than 10 days, either infected with MNV or left uninfected and then treated with 3% DSS in drinking water for 6 days. Intestinal epithelial cells were isolated from the colon and sorted and RNA extracted for RNASeq analysis. We found that mice with IFNAR expression on IEC and infected with MNV had a gene expression signature of DNA repair similar that induced by IL-22 signaling. Overall design: 3 mice in each group (IFNARf/f ABX, IFNARf/fxVillin-cre ABX, IFNARf/f ABX+CR6 and IFNARf/fxVillin-cre ABX+CR6)