DRP000160
Genome reconstruction of previously uncultivated lineages of thermophilic archaeotes
We selected putative archaeal genome fragments from the metagenomic library that was constructed from DNA assemblages isolated from a microbial mat at a geothermal water stream in a Japanese subsurface gold mine. Total of selected 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences.
A first composite genome sequence of "Caldiarchaeum subterraneus" that belongs to the previously uncultivated thermophilic creanrchaeotic lineage was successfully reconstructed.