GSE175563
RNA-seq analysis of mouse pluripotent stem cells derived cells
To detect the role of OP9 stromal cells in our optimized 3D self-assembling peptide induction system followed by the OP9 coculture system. First, we used RNA-seq to analyze mouse pluripotent stem cells derived total cells at day5 between the 3D+OP9 and 3D+0.1% group in our hematopoietic differentiation system.  In addition, to further evaluate hematopoietic transcriptome differences between Lin-Sca-1+c-kit+CD201+ cells and  Lin-Sca-1+c-kit+CD201- cells, we used RNA-seq to analyze  mouse pluripotent stem cells derived Lin-Sca-1+c-kit+CD201+ and Lin-Sca-1+c-kit+CD201- cells  at day5 in our 3D+OP9 hematopoietic differentiation system.Meanwhile,we used the mouse embryonic stem cell(mESC) as negative control and bone marrow derived Lin-Sca-1+c-kit+CD201+ and fetal liver derived Lin-Sca-1+c-kit+CD201+ as positive control. Overall design: two groups together:  3 sample of 3D+OP9 and 3 sample of 3D+0.1%gel;  five groups together :  3 sample of D5-LSKCD201+     3 sample of D5-LSKCD201- 3 sample of mESC    3 sample of  BM-LSKCD201+    3 sample of FL-LSKCD201+