GSE171935
GUIDE-Seq analysis of the designed gRNAs (SG1, SG2, SG3, SG4, SG7 and SG8)
Off-target (OT) analysis of different guide RNAs targeting PKLR gene. GUIDE-Seq analyses were done in HEK293 cells stably expressing Cas9 transfected with gRNA complexes, generated using Alt-R® CRISPR-Cas9 crRNA XT and Alt-R®CRISPR-Cas9 tracrRNA, and transfected with a dsODN tag Overall design: OT analysis of different guide RNAs targeting PKLR gene by GUIDE-Seq