GSE113891
Transcriptomic profile of circulating CD4+ T cells from TCM and TEM  memory compartments from donors vaccinated at birth either with whole or acellular Pertussis vaccine
In the mid-1990s vaccine-related side effects prompted the replacement of the whole Pertussis (wP) by a new acellular Pertussis vaccine (aP). Unexpectedly, whooping cough cases have recently increased, particularly in teenagers. Here we compared individuals born before 1995 and vaccinated in infancy with wP, with individuals born in 1996 or later and vaccinated with aP in infancy. Remarkably, we detected differences in the two populations in response to a contemporary aP booster, despite the fact that the original aP or wP priming occurred more than 18 years previously. The nature of the differences in T cells between aP and wP original priming was further investigated by evaluation of memory subset composition in respect to function, activation and exhaustion expression protein markers and RNA transcriptomic profiles of the PT-specific T cells from aP vs. wP donors. Transcriptomic profile of circulating CD4+ T cells from TCM and TEM  memory compartments from donors vaccinated at birth either with whole or acellular Pertussis vaccine. All donors were boosted with the aP vaccine  1-3 months before the PBMCs were collected. Overall design: RNA-sequencing of sorted CD3+CD4+ cells from central (TCM) or effector (TEM) memory  compartments from cryopreserved PBMC of 8 subjects vaccinated at birth with whole Pertussis vaccine and  8 subjects vaccinated at birth with acellular Pertussis vaccine. All donors were boosted with the aP vaccine  1-3 months before the PBMCs were collected. Stimulation with either Pertussis (PT) or Tetanus (TT) petides with a 24h AIM assay were used to capture and sort the antigen-specific cells (average of 10,000 cells per sample).