ena-STUDY-NATIONAL UNIVERSITY OF IRELAND GALWAY-20-06-2018-17:04:29:680-6180
Whole-genome sequencing to elucidate the possible mechanism of E. coli adaptation to a biocidal iodo-thyocyanate complex
Chemostat continuous culture systems were used to investigate adaptation of E. coli to a novel antimicrobial agent (ITC) and resistance to levofloxacin (LVX) under nutrient limiting environment. Three separate enrichment continuous cultures of E. coli ATCC 25922 were operated to select for ITC-adapted and LVX-resistant mutants, and compared with selection-free cultures. The derivative culture from the chemostat run under LVX-selection pressure showed resistance towards LVX, and yet, no tolerance was observed towards ITC. Chemostats operated with ITC-selection pressure yielded population with no altered susceptibility towards ITC, as well as LVX. These results revealed that ITC usage is not promoting tolerance to itself, and no cross-resistance to antibiotics. The aim of this study was to use whole-genome sequence analysis of polymorphic populations sampled over time and compare starter, intermediate and final populations to reveal possible mutational determinants of ITC and LVX resistance.
Chemostat continuous culture systems were used to investigate adaptation of E. coli to a novel antimicrobial agent (ITC) and resistance to levofloxacin (LVX) under nutrient limiting environment. Three separate enrichment continuous cultures of E. coli ATCC 25922 were operated to select for ITC-adapted and LVX-resistant mutants, and compared with selection-free cultures. The derivative culture from the chemostat run under LVX-selection pressure showed resistance towards LVX, and yet, no tolerance was observed towards ITC. Chemostats operated with ITC-selection pressure yielded population with no altered susceptibility towards ITC, as well as LVX. These results revealed that ITC usage is not promoting tolerance to itself, and no cross-resistance to antibiotics. The aim of this study was to use whole-genome sequence analysis of polymorphic populations sampled over time and compare starter, intermediate and final populations to reveal possible mutational determinants of ITC and LVX resistance.