Methylome_and_Hydroxymethylome_of_iPSC-sc-2013-09-18T12:13:20Z-2769
Methylome_and_Hydroxymethylome_of_iPSC
Induced Pluripotent Stem Cells (iPSC) were obtained from primary embryonic fibroblast form mice of different genetic backgrounds. DNA was obtained from cells with the Qiagen AllPrep kit. 0.25ug of DNA was sonicated with the Covaris Sonicator (300bp) and the Illumina libraries were prepared following the TrueMethyl 6 protocol and libraries amplified 10x.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The main goals of this project are to investigate the role of AID in reprogramming and characterize its effects in the reprogramming potential of induced pluripotent stem cells. This project aims to analyse genome-wide and locus-specific differences in methylation between iPSC of different genomic backgrounds.