DRP000222
Escherichia coli K-12 Transcriptome project
First, Escherichia coli cells were grown to late exponential phase (O.D.600 = 0.76) in modified M63 medium. Then, low molecular weight RNAs (<200 nt) were isolated from the cultured cells using mirVana miRNA Isolation Kit (Ambion). cDNA library was built from the RNA sample ligated with both 5' and 3' end specific RNA adapters for determining direction of each transcript. The cDNA library was deep sequenced by Illumina/Solexa 1G Genome Analyzer system.