GSE119872
Identification of regulators of vascular proliferation
Vascular tissue transports water and nutrients throughout the plant body and is consequently essential for growth and development. In Arabidopsis, the vascular tissue is initiated at the centre of the embryo.  During embryogenesis it is found in a characteristic diarch pattern with a central file of xylem cells with an adjacent layer of procambium on either side. Phloem poles are present on the outside of the procambium layers. Following germination a series of cell divisions results in the reorganisation of the vascular tissue which transitions to a radially symmetric structure. The RNA-seq data associated with this submission captures those early events. In 7-day old seedlings, wild type hypocotyl s have begun the process of change towards radial symmetry. In pxy mutants this process is stalled, and in 35S::CLE41 lines, radial expansion occurs prematurely. In root tissue of this age there is little evidence of the transition to radial symmetry in any of the genotypes tested. The RNA-seq dataset submitted will thus provide insight into the changes required to reorganise Arabidopsis vascular tissue as it transitions from primary to secondary growth. Overall design: There are 6 groups made up of 4 biological replicates each (24 Samples in total). Each sample has 2 technical replicates. Samples are taken from either the root or the shoot and there is a control group for both root and shoot groups (designated WT)