PRJNA269041
Integration of Developmental and Environmental Signals via a Polyadenylation Factor in Arabidopsis
The integration of environmental and developmental signals with physiological responses is critical for plant survival. One level of integration involves posttranscriptional controls, such as RNA processing. Previously, it was found that the 30 kD subunit of Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF30) is a calmodulin-regulated RNA-binding protein. In this study, genome-wide poly(A) site choice in wild-type plants, a mutant (oxt6) deficient in CPSF30 expression, a line in which CPSF30 (C30G) is expressed in the oxt6 background, and a line in which a calmodulin-binding mutant of CPSF30 (C30GM) is expressed in the oxt6 background, was determined. In addition, a number of oxt6-associated phenotypes were evaluated in the complemented lines. The phenotypic analyses show that the interaction of CPSF30 with calmodulin is important some, but not all, of the functions of the protein in the plant. The global poly(A) site analysis show that the C30G and C30GM proteins can restore wild-type poly(A) site choice to the oxt6 mutant. These results suggest that CPSF30 is a regulatory hub where different signaling cues are transduced into specified phenotypic outcomes.