ena-STUDY-HENAN UNIVERCITY-22-09-2020-14:07:45:277-164
Oryza sativa LIGULELESS 2s determine lamina joint positioning and differentiation by inhibiting auxin signaling
Leaf angle affects architecture and grain yield, especially in cereal crops. The lamina joint (LJ) is a grass-specific organ that determines leaf angle; however, the mechanism that underlies LJ organogenesis in rice is unknown. Here, we identified two LIGULELESS 2 (LG2) genes in rice, OsLG2 and OsLG2L, and demonstrated that they were essential to determine LJ positioning; and the positioning determination-induced OsLG1 initiated the subsequent LJ tissue differentiation. Using OsLG2-cri OsLG2L-cri and OsLG1-cri lines for transcriptome analysis, we found that OsLG2/2L and OsLG1 initiated LJ through inhibiting auxin signaling. In addition, OsLG2/2L and OsLG1 function in a dose-dependent manner in LJ organogenesis and leaf angle formation. This study elucidates the mechanism of LJ organogenesis by LG genes in rice and provides a foundation for crop improvement.
Leaf angle affects architecture and grain yield, especially in cereal crops. The lamina joint (LJ) is a grass-specific organ that determines leaf angle; however, the mechanism that underlies LJ organogenesis in rice is unknown. Here, we identified two LIGULELESS 2 (LG2) genes in rice, OsLG2 and OsLG2L, and demonstrated that they were essential to determine LJ positioning; and the positioning determination-induced OsLG1 initiated the subsequent LJ tissue differentiation. Using OsLG2-cri OsLG2L-cri and OsLG1-cri lines for transcriptome analysis, we found that OsLG2/2L and OsLG1 initiated LJ through inhibiting auxin signaling. In addition, OsLG2/2L and OsLG1 function in a dose-dependent manner in LJ organogenesis and leaf angle formation. This study elucidates the mechanism of LJ organogenesis by LG genes in rice and provides a foundation for crop improvement.