GSE70066
Transcriptome response of Chlamydomonas reinhardtii exposed to methylmercury (MeHg)
This study aims at investigating the effects of methylmercury on a microalga using a transcriptomic approach. Algal cells were exposed two hours in a simplified artificial medium spiked with 0.05 or 0.5 nM MeHg. Total RNA was extracted using TRI Reagent®. Libraries were prepared with the Illumina TruSeq Stranded mRNA kit and sequenced on an Illumina HiSeq 2500 System. Overall design: Three biological replicates were assessed for each of the 3 tested conditions: Control, 0.05 nM MeHg and 0.5 nM MeHg.