GSE137426
The fibronectin mediated endothelial rescue effect in CCM3-deficient cells is independent from acute mRNA changes
Using CRISPR/Cas9 genome editing and RNA sequencing, we demonstrate that fibronectin supplementation restores the impaired spheroid formation and attenuates actin stress fiber assembly in CCM3-/- endothelial cells independently of acute gene expression differences. In particular, there was no significant downregulation of well-known key players in CCM pathogenesis like KLF2 or KLF4. Overall design: Examination of the mRNA expression profile of 3 wild-type CI-huVEC lines and 3 clonal CCM3-knockout CI-huVEC lines with or without fibronectin treatment.