PRJNA725951
Intestinal epithelial MPST controls AKT-dependent apoptosis to regulate intestinal inflammation in UC
MPST expression was knocked down inHT29 cells by siRNAs for 24 h, and then the cells were stimulated with or without 100 ng/ml of TNF for 24 h.Total cellular RNA was extracted using TRIzolfrom 1x106 cells per duplicate and genomic DNA was removed using a DNA-free kit . Quality control and sequencing was done by Novogene Bioinformatics Technology Co.Ltd.