PacBio_sequencing_Escherichia_coli_sequence_type__ST_131_from_nursing_homes_in_Ireland-sc-4566
PacBio_sequencing_Escherichia_coli_sequence_type__ST_131_from_nursing_homes_in_Ireland
Escherichia coli sequence type (ST) 131 is globally disseminated and associated with antimicrobial resistance and with urinary tract and blood stream infections. From 2005 and 2011, we collected 69 ESBL E. coli ST131 isolates from 63 residents of a nursing home in Ireland. All isolates were sequenced using the Illumina Hiseq platform and compared to E. coli ST131 submitted to the referral hospital in 2010, from 8 hospitalised patients, 11 from other nursing homes and 2 from general practitioners. Genome-based comparison of 69 E. coli ST131 isolates from the individual nursing home demonstrated three distinct lineages. PacBio sequencing will be performed to compare the mobile elements that differ between the nursing home lineages.
Escherichia coli sequence type (ST) 131 is globally disseminated and associated with antimicrobial resistance and with urinary tract and blood stream infections. From 2005 and 2011, we collected 69 ESBL E. coli ST131 isolates from 63 residents of a nursing home in Ireland. All isolates were sequenced using the Illumina Hiseq platform and compared to E. coli ST131 submitted to the referral hospital in 2010, from 8 hospitalised patients, 11 from other nursing homes and 2 from general practitioners. Genome-based comparison of 69 E. coli ST131 isolates from the individual nursing home demonstrated three distinct lineages. PacBio sequencing will be performed to compare the mobile elements that differ between the nursing home lineages.