GSE106929
Next Generation Sequencing Facilitates Comparative Transcriptomes Analysis for different Yarrowia lipolytica strains synthesizing different levels of ß-carotene as end products.
Purpose: According to comparative transcriptomes data, the differentially expressed genes within these selected Y. lipolytica strains can be digged out and further analyzed, seeking potential targets that were tuned drastically and might improve ß-carotene production when modified. Methods: mRNA profiles of three Y. lipolytica strains ctrl, Y5_2, Y5_3 (each named as Yl_5_1, Yl_5_2, Yl_5_3 in related manuscript) were generated in triplicate using illumina. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) and index codes were added to each sample. The library preparations were sequenced on an Illumina Hiseq 4000 platform. 150 bp paired-end reads were generated as raw data. The read counts for each gene in the genome were summarized as processed data. Results: In all strain samples, the transcriptome data for all 6145 genes throughout genome were detected. The results showed that the expression levels of nearly half of the total genes were tuned to a higher or a lower value. Conclusions: There were 2848 differentially expressed genes between strain ctrl and Y5-2 and this number was 2889 for ctrl vs. Y5_3. The GO and KEGG enrichment of these differentially expressed genes would be further operated for exploring drastically regulated functions and pathways. Overall design: The transcirptomes of different Yarrowia lipolytica strains were sequenced by Illumina, giving both unchanged genes and differentially expressed genes that would affect ß-carotene production.