DRP002630
Rhizosphere microbial community manipulated by 2-year consecutive bio-fertilizer application exhibited excellent disease suppression to banana Fusarium wilt disease
2-year consecutive application of bio-fertilizer to a banana orchard with serious Fusarium wilt disease effectively controlled this soil-borne disease reported in our previous work. Deep pyrosequencing of 16S rRNA and ITS genes were performed to investigate how rhizosphere microbial community responded to application of bio-fertilizer (BIO), common pig manure compost (PM) and only chemical fertilizer (CF) and to explore the potential correlation between microbial community and Fusarium wilt disease suppression. In total 104,201 bacterial 16S and 154,953 fungal ITS sequence reads were obtained after basal quality control. Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Ascomycota were the most abundant bacterial and fungal phyla. The alpha diversity of bacteria was significantly increased while that of fungi significantly reduced after 2-year consecutive bio-fertilizer application. Acidobacteria and Firmucutes were significantly elevated while Proteobacteria and Ascomycota significantly depleted in the rhizosphere after bio-fertilizer application. Moreover, genera of Gp1, Gp3, Leptosphaeria and Phaeosphaeriopsis were also significantly enriched in BIO treatment compared to PM and CF treatments. Interestingly, Fusarium was also significantly reduced in BIO treatment compared to CF treatment and a slightly reduce without significance compared to PM treatment. Furthermore, the disease incidence was negatively correlated to the enrichment of Gp1, Gp3, Leptosphaeria and Phaeosphaeriopsis while positively correlated to depletion of Fusarium. In conclusion, the reduction of the Fusarium wilt disease incidence after a 2-year application of bio-fertilizer might be attributed to the fact that application of bio-fertilizer containing Bacillus sp. induced general suppression by modulating rhizosphere the microbial community.