ena-STUDY-NORWICH RESEARCH PARK-30-03-2020-13:41:28:644-10
Integrated long- and short-read single-cell RNA-sequencing
Single-cell RNA sequencing analysis has recently provided snapshots of gene expression of specific cell typesand enabled cell types classification within an heterogenous population. As well as transcriptional changes, alternative splicing events and modifications of components of splicing machinery actively contributes in shaping cellular phenotype as well as ageing process and diseases occurence.Current high-throughput single-cell RNA sequencing methods may lack  information on cell-specific isoform expression, missing key aspects of cell biology. In the present work we introduce a novel approach using the 10X Genomics Chromium to generate short-read (Illumina) and long-read (Pacific Biosciences Sequel II) RNA-sequencing libraries from the same single cells. This approach produced single cell parallel transcriptional and splicing profiling that demonstrates for the first time age, cell-type specific isoform expression and splicing alterations associated with ageing in haematopoietic stem and progenitor cells
Single-cell RNA sequencing analysis has recently provided snapshots of gene expression of specific cell typesand enabled cell types classification within an heterogenous population. As well as transcriptional changes, alternative splicing events and modifications of components of splicing machinery actively contributes in shaping cellular phenotype as well as ageing process and diseases occurence.Current high-throughput single-cell RNA sequencing methods may lack  information on cell-specific isoform expression, missing key aspects of cell biology. In the present work we introduce a novel approach using the 10X Genomics Chromium to generate short-read (Illumina) and long-read (Pacific Biosciences Sequel II) RNA-sequencing libraries from the same single cells. This approach produced single cell parallel transcriptional and splicing profiling that demonstrates for the first time age, cell-type specific isoform expression and splicing alterations associated with ageing in haematopoietic stem and progenitor cells