Trypanosoma_brucei_TAR_clone_sequencing-sc-2011-08-02T12:36:25Z-1925
Trypanosoma_brucei_TAR_clone_sequencing
The telomeres of Trypanosoma brucei are underrepresented in standard libraries so a strategy for isolating T. brucei BES-containing telomeres in Saccaromyces cerevisiae by using transformation-associated recombination (TAR) was devised (Becker M., Aitcheson N., Byles E., Wickstead, B., Louis E. and Rudenko G. (2004) Isolation of the repertoire of VSG expression site containing telomeres of Trypanosoma brucei 427 using Transformation Associated Recombination in yeast. Genome Research 14: 2319-2329.). In this approach, rather than just sequencing the TAR clone, we are sequencing the yeast genome plus telomeric YACs from Trypanosoma brucei 927 to capture missing telomeric sequence. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Illumina sequencing of  Saccharomyces cerevisiae plus telomeric YACs from Trypanosoma brucei 927.