PRJNA760795
Establishment of trophoblast stem cells from conventional (primed) human pluripotent stem cells
Human pluripotent stem cells (hPSC) cultured in conventional conditions involving the growth factor FGF2 and Activin/NODAL signaling are generally considered to correspond developmentally to post-implantation epiblast ("primed" for differentiation into the three somatic lineages endoderm, mesoderm and exctoderm) and therefore incapable of differentiating along the trophectoderm/trophoblast lineage. We developed a protocol using chemically defined culture conditions that leads to differentiation of hPSC into trophoectoderm, establishment of trophoblast stem cells capable of terminal differentiation into extravillous trophoblast and syncytiotrophoblast. This project contains multiple datasets - bulk, single-cell RNA-seq, miRNA-seq and MeDIP-seq. Bulk RNA-seq datasets include: 1. time-course of induction of hPSC into trophectoderm, 2. comparison of hPSC-derived trophectoderm with somatic lineages (ectoderm, mesoderm, endoderm) and choriocarcinoma cell lines, 3. transcriptomic analysis of trophoblast stem cells. Single-cell RNA-seq datasets include: 1. time-course of induction of hPSC into trophectoderm, 2. single-cell analysis of trophoblast stem cells. miRNA-seq and MeDIP-seq datasets contain miRNA expression profiles of hPSC, trophectoderm and trophoblast stem cells.