GSE211339
Effect of CuCl2 on genomic ssDNA regions in A549 cells
To investigate the genomic effects of CuCl2 on A549 cells in vitro, we treated cells with sDMEM (DMEM + 10% FBS + 1% Pen/Strep cocktail) or sDMEM + CuCl2 for various timepoints at various concentrations (or untreated cell control) followed by kethoxal labeling of single stranded DNA (ssDNA) We then performed differential analysis of ssDNA levels following the KAS-pipe pipeline Overall design: Comparative ssDNA profiling analysis of KAS-seq data for human A549 cells treated with CuCl2 vs. untreated cells.