PRJNA835242
Anser cygnoides domesticus strain:Chinese local goose | breed:Sichuan White Goose Raw sequence reads
Goose liver has the particularity of lipid deposition, short-term feeding produces fatty liver, and the applicant found that the embryo Lipid droplet deposition in the normal liver during pregnancy. Given the special biological phenomenon of goose liver lipid deposition, The applicant focuses on the scientific proposition of "transcriptional regulation mediated by spatial conformational changes of chromatin", to be exhibited do in-depth research. Select embryonic stages (embryo stages 9, 13, 17, and 21) were used as experimental materials.Multi-omics technologies such as Hi-C, ATAC-seq, and RNA-seq to analyze chromatin conformation, chromatin Continuous changes in openness and gene expression during the above process, identification, and clarification of three-dimensional genes Groups of hierarchical topological structural units (chromatin domains, compartments, topological association domains, and chromatin loops) Mediated patterns of long-range interactions between transcripts and regulatory elements (enhancers and insulators); in Transcriptional regulators identified in primary goose hepatocytes using co-immunoprecipitation and luciferase reporter assays Control elements and their target genes to clarify specific biological effects.