E-MTAB-10018
Single cell RNA sequencing of naive and primed human pluripotent stem cells treated with SB431542
The aim of this experiment was to investigate the role of TGFß signalling in human pluripotency, looking at the effect of SB431542 (SB), a potent and selective SMAD inhibitor that blocks TGFß/Activin receptors ALK5, ALK4, and ALK7, on gene expression. We performed 10X sequencing in naïve and primed human pluripotent stem cells (hPSCs) during a time-course of SB treatment.
The aim of this experiment was to investigate the role of TGFß signalling in human pluripotency, looking at the effect of SB431542 (SB), a potent and selective SMAD inhibitor that blocks TGFß/Activin receptors ALK5, ALK4, and ALK7, on gene expression. We performed 10X sequencing in naïve and primed human pluripotent stem cells (hPSCs) during a time-course of SB treatment.