E-MTAB-6809
RNA-Seq of sorted and unsorted prophage-induced subpopulations in Corynebacterium glutamicum
Cells were treated as described in the material and methods section of the manuscript. Briefly, RNA-seq experiments were performed with sorted populations with an size of about 10_E6 Corynebacterium glutamicum cells. As a reference data set RNA-Seq. analysis of unsorted cells were conducted (n=3, induced vs uninduced =2, 2 x 3 = 6 data sets). For the sorting and the counter-silencer based prophage induction, RNAprotect and RNAlater were used and for each agent data sets with two biological relicates were generated (n = 2, RNAprotect + RNAlater = 2, induced vs uninduced  = 2, 2 x 2 x 2 = 8 data sets). One data set was generated using 10_E5 cells (n = 1, induced vs uninduced =2, 2 x 1 = 2  data sets) For the subpopulation caused by iron-shift experiments RNAprotect was used (n = 3, positive vs negative, 3 x 2 = 6 data sets). In sum 26 data sets are uploaded. Each consist of two fastq files (due to paired-end sequencing).
Cells were treated as described in the material and methods section of the manuscript. Briefly, RNA-seq experiments were performed with sorted populations with an size of about 10_E6 Corynebacterium glutamicum cells. As a reference data set RNA-Seq. analysis of unsorted cells were conducted (n=3, induced vs uninduced =2, 2 x 3 = 6 data sets). For the sorting and the counter-silencer based prophage induction, RNAprotect and RNAlater were used and for each agent data sets with two biological relicates were generated (n = 2, RNAprotect + RNAlater = 2, induced vs uninduced  = 2, 2 x 2 x 2 = 8 data sets). One data set was generated using 10_E5 cells (n = 1, induced vs uninduced =2, 2 x 1 = 2  data sets) For the subpopulation caused by iron-shift experiments RNAprotect was used (n = 3, positive vs negative, 3 x 2 = 6 data sets). In sum 26 data sets are uploaded. Each consist of two fastq files (due to paired-end sequencing).