PRJNA542607
freshwater sediment metagenome Raw sequence reads
The field survey was conducted at Xinxue River mCW in February 2017. In this field survey, surface sediments and overlying water samples were collected from 6 sites (C0, C1, C2, C3, C4, C5) at retention pond export (117°9'8''E,34°45'59''N), primary wetland export (117°9'8''E,34°45'59''N), secondary wetland export (117°8'56''E,34°46'7''N), tertiary wetland export (117°8'44''E,34°45'40''N), quartus wetland export (117°8'23''E,34°45'1''N) and fifth wetland export (117°9'9''E,34°44'17''N). At each site, the overlying water samples were gathered with Niskin bottles and filtered through 0.45µm syringe filters. The surface sediment samples were collected with a Peterson mud sampler by taking 0-10 cm depth and put in a sterile polyethylene plastic bag under 4 o C. The samples were transported to the laboratory for the follow-up experiments. Then, sediment samples were freeze-dried and sieved for further analysis.