ena-STUDY-CFB-Yeast metabolic engineering-11-06-2019-07:43:01:885-143
Genome resequencing of a engineered Saccharomyces cerevisiae strain ST8424 and its UV mutant for the beneficial mutations for improved muconic acid production.
Genome resequencing of a engineered Saccharomyces cerevisiae strain ST8424 and its UV mutant for the beneficial mutations for improved muconic acid production.Details regarding library preparation and sequencing:• Sequencing facility:o NGS Lab at the Novo Nordisk Foundation Center for Biosustainability• Library preparation kit:o KAPA HyperPlus Library Prep Kit (ROCHE)• Indexing kit:o Dual Indexed PentAdapters, Illumina compatible (PentaBase)• Quality control:o Qubit dsDNA HS Assay Kit (Invitrogen); used for quantification of input DNA and sequencing librarieso DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp - 6000 bp); (Agilent); used to assess the average size of the sequencing libraries• Average sequencing library size:o 405 bp• Sequencing instrument:o NextSeq 500 (Illumina)• Sequencing kit(s):o NextSeq Mid Output v2 Kit (300 Cycles) (Illumina)• Sequencing reads:o 150 base pair paired-end (2x150 bp)• Index (i7 and i5) reads:o 8 bp, dual indexed• Flow cell loading:o 1.05 pM• Sequencing chemistry:o 2-channel sequencing-by-synthesis (SBS) technology (NS)• Use of PhiX control v3:o Yes; added at 2.5 %
Genome resequencing of a engineered Saccharomyces cerevisiae strain ST8424 and its UV mutant for the beneficial mutations for improved muconic acid production.Details regarding library preparation and sequencing:• Sequencing facility:o NGS Lab at the Novo Nordisk Foundation Center for Biosustainability• Library preparation kit:o KAPA HyperPlus Library Prep Kit (ROCHE)• Indexing kit:o Dual Indexed PentAdapters, Illumina compatible (PentaBase)• Quality control:o Qubit dsDNA HS Assay Kit (Invitrogen); used for quantification of input DNA and sequencing librarieso DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp - 6000 bp); (Agilent); used to assess the average size of the sequencing libraries• Average sequencing library size:o 405 bp• Sequencing instrument:o NextSeq 500 (Illumina)• Sequencing kit(s):o NextSeq Mid Output v2 Kit (300 Cycles) (Illumina)• Sequencing reads:o 150 base pair paired-end (2x150 bp)• Index (i7 and i5) reads:o 8 bp, dual indexed• Flow cell loading:o 1.05 pM• Sequencing chemistry:o 2-channel sequencing-by-synthesis (SBS) technology (NS)• Use of PhiX control v3:o Yes; added at 2.5 %