PRJNA827854
Impacts of low temperature on the transcriptome of two invasive fruit fly species, Bactrocera dorsalis (Hendel) and Bactrocera correcta (Bezzi) (Diptera: Tephritidae)
Temperature is one of the basic conditions of insect life activities, many insects have the ability to improve their resistance to low temperatures by undergoing cold hardening at nonlethal temperatures. Both Bactrocera dorsalis and B. correcta are important invasive fruit flies. Predecessors found that the two fruit flies can also adapt to a certain range of low temperature, which increases the potential diffusion risk of the two fruit flies and the possibility of establishing a stable population. However, the molecular mechanism of its low temperature response is not clear. Therefore, we explored the molecular mechanism of low temperature adaptation of these two fruit flies. We conducted transcriptome analysis between pupae of B. dorsalis and B. correcta using RNA-Seq. The results showed that 152 DEGs were up-regulated in B. dorsalis and 525 differentially expressed genes in B. correcta under low temperature . Go enrichment analysis suggested that the positive regulation of leukocyte and cell-cell adhesion, immune system process, extracellular matrix, chitin-based cuticle, heat shock protein and ribosome were significantly enriched under low temperature. Among the DEGs, we found that some heat shock protein (HSP) genes and cuticular protein (CP) genes respond positively to low temperature, which may be related to low temperature adaptability. It is noteworthy that a candidate gene Hsp70 gene is significantly up-regulated in both B. dorsalis and B. correcta, and Hsp 23 , HSPsp90ABab1, CPRpr1 waswere also found to be sensitive to low temperature in B. dorsalis. Similarly, some other key genes have been found in guava fruit flies, which are Hsp68, Lcp65Ag1, Acp1, etc. The results provide basic data for the follow-up bioinformatics analysis, the discovery of key genes of low temperature tolerance of these two Bactrocera species and the disclosure of low temperature adaptation mechanism.