GSE159121
Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing
Background: Osteoporosis (OP) is a systemic disease with bone loss and microstructural deterioration. Numerous noncoding RNAs have been proved to participate in various diseases, especially for circular RNAs (circRNAs). However, the expression profile and hidden mechanisms of circRNAs in male osteoporosis have not been explored yet. Methods: Here we investigated the whole transcriptome expression profile and differences of mRNAs, circRNAs and miRNAs in peripheral blood samples of patients with osteoporosis and healthy controls of male with age larger than 60 years old. Results: A total of 398 circRNAs, 51 miRNAs and 642 mRNAs were identified that were significant differentially expressed in osteoporosis compared with healthy controls. GO and KEGG enrichment analysis showed the host genes of significantly differentially expressed circRNAs were mainly enriched in regulation of cell cycle process BP, organelle part CC, protein binding MF and Toll-like receptor signaling pathway, TNF signaling pathway and Thyroid hormone signaling pathway. circRNA-miRNA-mRNA regulatory network was constructed using the differentialy expressed RNAs. Moreover, key circRNAs in osteoporosis were discovered further and validated by qPCR, such as hsa_circ_0042409. Those key cicrRNAs may play an important role in the pathogenesis of osteoporosis and could be used as potential biomarkers or targets in the diagnosis and treatment of osteoporosis. Overall design: A total of 6 peripheral blood samples were collected, including 3 healthy controls (NORM1, NORM2, NORM3) and 3 patients with osteoporosis diagnosed (OP1, OP2, OP3)