Genome_wide_CRISPR_gRNA_screens_with_Mouse_v2_library-sc-3272
Genome_wide_CRISPR_gRNA_screens_with_Mouse_v2_library
PCR products were generated using genome-wide CRISPR libraries as template DNA. Subsequently, PCR products are pooled and subjected to Illumina library preparation.  The library will be sequenced by HiSeq2500 RapidRun.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ submitted for publication. For information on the proper use of data shared by the Wellcome Trust Sanger Institute (including information on acknowledgement), please see http://www.sanger.ac.uk/datasharing/
The CRISPR-Cas system is a new DNA-editing technology and promise potential in many applications such as genome engineering and genetic screening. We are performing genome-wide CRISPR guide RNA screening in multiple cell lines to identify genes that are essentail for cell survival. We would like to sequence guide RNAs to idnetify such genes.