GSE161872
Single-cell transcriptional profiles in the visceral adipose tissue from obese and lean C57BL6 mice
Cellular senescence has been reported to play an important role in insulin resistance. While the molecular mechanism remains unclear. Here we applied  the scRNA-seq analysis for the visceral fat from the obese mice with 2 month of high-fat diet. Visceral fat was digested by type II collagenase. Stromal Vascular  Fraction was collected, incubated with hashing antibody and then subjected to the 10X Chromium. 8180 cells were found from the obese mice and 14,502 cells were from the lean mice. 14 clusters and 9 cell types were identified. The key senescence marker  P21 were found  highly expressed in the obese group, when compared to the lean group. However, the other senescence marker P16 were unchanged. P21+ cells are mainly preadipocytes, macrophage and endothelial cells.  More than 300 different expressed genes were found among the P21+ cells. The NF-kB pathway was activate according to the GO analysis, which is strongly associated with insulin resistance and diabetes Overall design: Examnination of the senescence in the insulin resistance condition