PRJNA528505
16S gut microbiota from nociceptor-ablated mice
The goal of this 16S microbiota study was to analyze gut bacterial community structures in nociceptor-ablated mice in different contexts. To ablate nociceptors, three strategies were used. First, Nav1.8-Cre mice were bred with Rosa26-Diptheria toxin (DTA-floxed) mice to generate Nav1.8-Cre/DTA reporter progeny, where Nav1.8-lineage sensory neurons are ablated during development (Cre+ mice) compared with nociceptor-sufficient control littermates (Cre- mice). Second, TRPV1-expressing neurons were chemically ablated by injecting wildtype mice with resiniferatoxin (RTX), a potent TRPV1 ligand that leads to neuronal denervation. Control wildtype mice were injected with vehicle (Veh). Third, TRPV1-Cre mice were bred with Rosa26-Diptheria toxin (DTA-floxed) mice to generate TRPV1-Cre/DTA reporter progeny, where TRPV1-lineage sensory neurons are ablated during development (Cre+ mice) compared with nociceptor-sufficient control littermates (Cre- mice). This dataset contains 288 samples spread across 5 different experiments described below.Experiment 1 (barcodes 0-89): Does TRPV1 neuron ablation influence the gut microbiota during Salmonella typhimurium infection?-Feces were collected at day 0 (before infection), and day 1 and day 5 (post-infection) from 13 vehicle-treated and 12 RTX-treated mice-Ileum mucosa was collected at day 5 post-infection from 8 vehicle-treated and 7 RTX-treated miceExperiment 2 (barcodes 240-279): Does Nav1.8 neuron ablation influence the gut microbiota during Salmonella typhimurium infection?-Feces were collected at day 0 (before infection), and day 1 and day 5 (post-infection) from 5 Nav1.8-Cre-/DTA and 5 Nav1.8-Cre+/DTA mice-Ileum mucosa was collected at day 5 post-infection from 5 Nav1.8-Cre-/DTA and 5 Nav1.8-Cre+/DTA miceExperiment 3 (barcodes 101-196): Does Nav1.8 neuron ablation influence the gut microbiota in different gut segments at homeostasis?-Feces were collected at 4 and 10 weeks of age of single-housed mice from 6 Nav1.8-Cre-/DTA and 6 Nav1.8-Cre+/DTA mice-Duodenum lumen and duodenum mucosa was collected from 6 Nav1.8-Cre-/DTA and 6 Nav1.8-Cre+/DTA mice at 10 weeks of age-Ileum lumen and ileum mucosa was collected from 6 Nav1.8-Cre-/DTA and 6 Nav1.8-Cre+/DTA mice-Colon lumen and colon mucosa was collected from 6 Nav1.8-Cre-/DTA and 6 Nav1.8-Cre+/DTA miceExperiment 4 (barcodes 200-239): Does TRPV1 nociceptor activation by capsaicin-diet influence the gut microbiota?-Feces were collected before the diet regimen from 10 control chow-fed and 10 capsaicin chow-fed wildtype mice-Feces were collected after 4 weeks of feeding from 10 control chow-fed and 10 capsaicin chow-fed wildtype miceExperiment 5 (barcodes 280-295, and barcodes 90-93): Does TRPV1 neuron ablation influence the gut microbiota at homeostasis?-Feces were collected at 7 weeks of age of single-housed mice from 10 TRPV1-Cre-/DTA and 10 TRPV1-Cre+/DTA mice