ena-STUDY-ENS Lyon-13-03-2017-09:58:10:809-295
Mapping-by-sequencing data for mutant allele mf35 (Oscheius tipulae)
The Oscheius tipulae strain JU54 carries the recessive mutation mf35 in the CEW1 background (reference genome). mf34 is an allele of the locus cov-3, published in Louvet-Vallee S. et al., 2003 (PMID:12586702).In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 21 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.
The Oscheius tipulae strain JU54 carries the recessive mutation mf35 in the CEW1 background (reference genome). mf34 is an allele of the locus cov-3, published in Louvet-Vallee S. et al., 2003 (PMID:12586702).In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 21 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.