GSE125458
A TRIM71 binding long noncoding RNA Trincr1 represses FGF/ERK signaling in embryonic stem cells
Long noncoding RNAs (lncRNAs) have emerged as important components of gene regulatory network in embryonic stem cells (ESCs). However, the function and molecular mechanism of lncRNAs are still largely unknown. Here we identified Trincr1 (TRIM71 interacting long noncoding RNA 1) lncRNA that regulates the FGF/ERK signaling and self-renewal of ESCs. Trincr1 is exported by THOC complex to cytoplasm where it binds and represses TRIM71, leading to the downregulation of SHCBP1 protein. Knocking out Trincr1 led to the upregulation of phosphorylated ERK and ERK pathway target genes and the decrease of ESC self-renewal, while knocking down Trim71 completely rescued the defects of Trincr1 knockout. Furthermore, ectopic expression of Trincr1 repressed FGF/ERK signaling and the self-renewal of neural progenitor cells. Together, this study reveals novel regulators in FGF/ERK signaling pathway and highlights lncRNA as an important player in cell signaling network to coordinate cell fate specification. Overall design: Examination of differential expressed genes between WT and Trincr1-ko mouse embryonic stem cells grown in 2iL and PD+LIF media by poly(A)+ RNA-Seq. Two biological replicates were completed for each groups.Identification of Trim71 binding RNA were investigated by RIP-seq. Empty cells were used as negative control. Two biological replicates were completed for each groups.