PRJNA558213
Bombyx mori Raw sequence reads
DNA methylation is an important epigenetic modification in eukaryotes. 5-methylcytosine (5-mC) is the most methylation modification in genomic DNA. In mammals, 5mC mainly presents in the promoters of genes, functioning the inhibition of gene expression. While in insects, 5mC mainly presents in genebody. Although 5mC was demonstrated affecting Oncopeltus fasciatus ovarian development by methyltransferase 1 (Dnmt1) RNAi, why insects remain intragenic 5mC and how it regulates gene expression to affect insect reproduction are unknown. To investigate whether 5mCs in ovarian gene bodies regulate gene expression, the relationship between gene 5mC levels in the ovarian genome and gene expression in ovarian transcriptome was analyzed