Microbial zonation in a bioreactor modeling a souring low temperature oil reservoir subjected to nitrate injection.
Microbial zonation in a bioreactor modeling a souring low temperature oil reservoir subjected to nitrate injection.
Microbial diversity analysis of fractions collected across the flow pack of a bioreactor injected with sulfate and nitrate continuously using next-generation 454. pyrosequencing.
Investigations of the microbial communities present in oil and gas reserves have traditionally relied on culture based techniques. Traditional culture based approaches to environmental microbiology can miss up to 90% of the indigenous community, only culture-independent approaches (metagenomics) can capture the  true indigenous microbial diversity. The objective of our study is to generate, develop, deploy and store metagenomic database of the microbial communities found in hydrocarbon resource environments. In order  to achieve this, next-generation pyrosequencing technologies were used for a 16S rRNA gene-based profiling of  microbial communities in a variety of hydrocarbon resource environments.