PRJNA450930
Chromatin-seq and RNA-seq of Drosophila melanogaster primary spermatocytes mutant for TMAC and tTFIID components and S2R+ cells
Primary spermatocytes from wild type flies (2 biological replicates), and flies mutant for comr, mip40, achi/vis, and nht, and Drosophila sperm were hand dissected followed by chromatin digestion by Micrococcal nuclease, or for total RNA purification. In addition, early spermatocytes, late spermatocytes and spermatids were seperated by hand, followed by RNA purification and sequencing, and S2R+ cells (3 biological replicates) were harvested for chromatin digestion and RNA purification. Purified DNA or RNA was then sequenced using an Illumina HiSeq 2000 machine.