ena-STUDY-Genoscope CEA-26-06-2017-15:30:12:756-306
De novo and updated assembly and annotation of L. biglobosa brassicae G12-14 using Oxford Nanopore MinION sequencing
L. biglobosa  G12-14 assembly was generated de novo using Oxford Nanopore MinION reads. Their scaffold N50 was of 383 kb and its lengths of 43.42 Mb. Its genome annotation was supported by integrating conserved proteins and RNA sequencing from Leptosphaeria-infected cotyledons, leaf stem, stem and canker-affected lower stem from canola (Brassica napus cv. Darmor and Bristol). It resulted in 14026 predicted protein-coding genes for L. biglobosa  G12-14. Genome completeness was estimated using BUSCO and reached 96% and 96% in respect to the eukaryotic BUSCO database.The newly produced assemblies and annotations of Leptosphaeria biglobosa G12-14 genomes will allow further studies, notably focused on the interaction between Leptosphaeria species and canola. The discovery of as yet unknown effectors will notably allow progress in B. napus breeding towards L. maculans resistance.Keywords: Leptosphaeria, genomics, assembly, annotation, long reads, Nanopore
L. biglobosa  G12-14 assembly was generated de novo using Oxford Nanopore MinION reads. Their scaffold N50 was of 383 kb and its lengths of 43.42 Mb. Its genome annotation was supported by integrating conserved proteins and RNA sequencing from Leptosphaeria-infected cotyledons, leaf stem, stem and canker-affected lower stem from canola (Brassica napus cv. Darmor and Bristol). It resulted in 14026 predicted protein-coding genes for L. biglobosa  G12-14. Genome completeness was estimated using BUSCO and reached 96% and 96% in respect to the eukaryotic BUSCO database.The newly produced assemblies and annotations of Leptosphaeria biglobosa G12-14 genomes will allow further studies, notably focused on the interaction between Leptosphaeria species and canola. The discovery of as yet unknown effectors will notably allow progress in B. napus breeding towards L. maculans resistance.Keywords: Leptosphaeria, genomics, assembly, annotation, long reads, Nanopore