GSE192797
Thyroid hormone receptor agonist promotes hair growth in mice
We report the application of RNA-sequencing technology for topical application of the thyroid hormone receptor agonist (TDM10842) in murine skin tissue. Twenty-nine 8-week-old C3H mice were obtained, and randomly divided into 3 groups, after depilation, with topical use of TDM, Vehicle and Blank respectively. We found 857, 782, and 276 differentially expressed genes were identified from the skin tissue between 3 groups. Weighted correlation network analysis (WGCNA) was used to find clusters of highly correlated genes from the 3 groups. From which, we found one gene, Pclaf, as a differentially expressed gene compared with the other two groups. We scored their pathway activities. We found that signaling pathway of E2F TARGETS, G2M CHECKPOINT, MYC TARGET V1, HEDGEHOG SIGNALING, MYC TARGET V2, WNT BETA CATENIN SIGNALING, EPITHELIAL MESENCHYMAL TRANSITION, MITOTIC SPINDLE, UNFOLDED PROTEIN RESPONSE and DNA REPAIR were up regulated in the TDM group compared with the other 2 control groups. Overall design: RNA-seq in mice skin tissue after topical application of the thyroid hormone receptor agonist.