ena-STUDY-FISABIO- Public Health Research-09-09-2016-11:52:35:054-171
Effect of gut microbiota composition on precancerous anal lesions
We studied men were HIV-infected undergoing screening for HPV-related precancerous lesions. We analyzed the microbiota composition of biopsy and fecal samples. We obtained the total DNA from these samples and we used it as template to amplify by PCR the V3-V4 region of 16S rRNA gene.The PCR products were sequenced using  MiSeq V3 (2x300 cycles)(Illumina).
We studied men were HIV-infected undergoing screening for HPV-related precancerous lesions. We analyzed the microbiota composition of biopsy and fecal samples. We obtained the total DNA from these samples and we used it as template to amplify by PCR the V3-V4 region of 16S rRNA gene.The PCR products were sequenced using  MiSeq V3 (2x300 cycles)(Illumina).