ena-PROJECT-UMCU-01-02-2013-07:33:48:219-17
Whole genome sequence of Staphylococcus aureus S0462
Background. The change of the bacteria from colonizers to pathogens is accompanied by a drastic change in expression profiles. These changes may be due to environmental signals or to mutational changes. We therefore compared the whole genome sequences of a set of S. aureus isolates from the same patient.   Methods. Two Staphylococcus aureus isolates (S0460 and S0462) from the farmer and a pig who were both carrier were obtained from the same farm at the same time. Sequencing was performed using 454 technology. The isolate’s SNPs in polyA/T tracts are excluded. Remaining SNPs are deemed true when supported by at least 5 reads; at least 90% SNP agreement between reads; SNP position in the contig is >50 bp from the border of the contig; quality score assigned by 454 sequencing.
Background. The change of the bacteria from colonizers to pathogens is accompanied by a drastic change in expression profiles. These changes may be due to environmental signals or to mutational changes. We therefore compared the whole genome sequences of a set of S. aureus isolates from the same patient.   Methods. Two Staphylococcus aureus isolates (S0460 and S0462) from the farmer and a pig who were both carrier were obtained from the same farm at the same time. Sequencing was performed using 454 technology. The isolate’s SNPs in polyA/T tracts are excluded. Remaining SNPs are deemed true when supported by at least 5 reads; at least 90% SNP agreement between reads; SNP position in the contig is >50 bp from the border of the contig; quality score assigned by 454 sequencing.