PRJDB8491
Transcriptome analysis to reveal roles of candidate genes responsible for the retinal development
The retina is composed of six types of neuron and one type of glial cell. The retinal cells are developed from common retinal progenitors in a chronologically defined order. Although key transcription factors are known to regulate the retinal development, the epigenetic molecular mechanisms underlying the retinal development remain unclear. Based on our previous RNA-sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, we selected several candidate genes for the retinal development. In this project, to reveal the roles of the candidate genes, we performed loss-of-function and gain-of-function analyses, and investigated the comprehensive gene expression profile by RNA-seq after purification of a certain type of cell.