ena-STUDY-GOTHENBURG UNIVERISITY-11-12-2014-15:14:28:007-352
Characterisation of the mouse gut microbiota using 16S rRNA gene 454 pyrosequencing data.
Two C57BL/6 mice colonies maintained in two rooms in the same specific pathogen free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus was similar in the two colonies. However, one colony had mucus that was impenetrable to bacteria or beads the size of bacteria, which is comparable to what we observed in free-living wild mice- whereas the other colony had an inner mucus layer penetrable to bacteria and beads. The different properties of the mucus in mice from the two rooms depended on the microbiota, as they were transmissible by transfer of caecal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, while mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus, our study shows that bacteria and their community structure affect mucus barrier properties in ways that can have implications for health and disease. It also highlights that genetically identical animals housed in the same facility can have rather distinct microbiotas and barrier structures.
Two C57BL/6 mice colonies maintained in two rooms in the same specific pathogen free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus was similar in the two colonies. However, one colony had mucus that was impenetrable to bacteria or beads the size of bacteria, which is comparable to what we observed in free-living wild mice- whereas the other colony had an inner mucus layer penetrable to bacteria and beads. The different properties of the mucus in mice from the two rooms depended on the microbiota, as they were transmissible by transfer of caecal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, while mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus, our study shows that bacteria and their community structure affect mucus barrier properties in ways that can have implications for health and disease. It also highlights that genetically identical animals housed in the same facility can have rather distinct microbiotas and barrier structures.