ena-STUDY-Universidad de las Islas Baleares-22-10-2020-11:06:16:607-157
Pseudomonas   arcuscaelestis  from Rainbow Trout
Strains P66: group E; were Gram-stain-negative, short rods, motile by more than one polar flagellum and isolated from Onchorhynchus mykiss, rainbow trout and farm water. The 16S rRNA sequence similarity values grouped them in the P. putida phylogenetic group of species. The strains were characterized phenotypically by the API 20NE and BIOLOG GENIII tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and by their fatty acid contents. Multilocus sequence analysis with four housekeeping gene sequences (rpoD, rpoB, gyrB and 16S rRNA) together with the genome comparisons by ANIb and GGDC values indicated that these strains cluster together in the phylogenetic analysis and their similarity values were lower than the thresholds established for species in the genus Pseudomonas. These data permit us to conclude that strains P66, P67, V1 and W15Feb18 belong to a novel species in the genus Pseudomonas for which the name Pseudomonas arcuscaelestis sp. nov. are proposed. The type strains are P66T.
Strains P66: group E; were Gram-stain-negative, short rods, motile by more than one polar flagellum and isolated from Onchorhynchus mykiss, rainbow trout and farm water. The 16S rRNA sequence similarity values grouped them in the P. putida phylogenetic group of species. The strains were characterized phenotypically by the API 20NE and BIOLOG GENIII tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and by their fatty acid contents. Multilocus sequence analysis with four housekeeping gene sequences (rpoD, rpoB, gyrB and 16S rRNA) together with the genome comparisons by ANIb and GGDC values indicated that these strains cluster together in the phylogenetic analysis and their similarity values were lower than the thresholds established for species in the genus Pseudomonas. These data permit us to conclude that strains P66, P67, V1 and W15Feb18 belong to a novel species in the genus Pseudomonas for which the name Pseudomonas arcuscaelestis sp. nov. are proposed. The type strains are P66T.