ena-STUDY-Institute for Bioengeneering and Biosciences-18-03-2017-20:10:11:489-286
Burkholderia isolates collected from a chronically infected cystic fibrosis patient over a period of 3.5 years, until the patient's death
Eleven Burkholderia cenocepacia clonal variants were collected from a single chronically infected cystic fibrosis patient, from the beginning of the infection until the patient's death with cepacia syndrome 3.5 years later. One Burkholderia multivorans co-infecting isolate from the same patient was also added to the study. The isolates were sequenced using Illumina, with a higher depth of coverage for 4 isolates. These longitudinal isolates have been thoroughly characterised phenotypically and at the level of the proteome, transcriptome and virulence. Coupled with detailed information on the patient's condition, disease progression and treatment management, the genomic analysis is aimed at identifying potentially adaptive mutations during chronic infection. This work is the result of a collaboration between the Institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, Portugal, and the Cystic Fibrosis Centre of Hospital Santa Maria in Lisbon, Portugal.
Eleven Burkholderia cenocepacia clonal variants were collected from a single chronically infected cystic fibrosis patient, from the beginning of the infection until the patient's death with cepacia syndrome 3.5 years later. One Burkholderia multivorans co-infecting isolate from the same patient was also added to the study. The isolates were sequenced using Illumina, with a higher depth of coverage for 4 isolates. These longitudinal isolates have been thoroughly characterised phenotypically and at the level of the proteome, transcriptome and virulence. Coupled with detailed information on the patient's condition, disease progression and treatment management, the genomic analysis is aimed at identifying potentially adaptive mutations during chronic infection. This work is the result of a collaboration between the Institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, Portugal, and the Cystic Fibrosis Centre of Hospital Santa Maria in Lisbon, Portugal.