E-MTAB-9445
RNA-seq of a time series experiment of leaf 3 tissue (primordia / leaf blade) of Arabidopsis plants grown under EoD FR since day 6, since day 18 or under WL conditions.
This RNA-seq was designed to help gain understanding on the genetic program behind phytochrome developmental time dependent control of leaf 3 (L3) cell proliferation and expansion phases, to ultimately regulate organ growth. Briefly, Arabidopsis thaliana Col-0 plants were grown under a light : dark (LD) 12 h : 12 h photoperiod, at a 100 µmoles/m2/s fluence rate and 21 °C of constant temperature. Plants were then exposed to either daily End of Day (EoD) far-red (FR, 40µmoles/m2/s of FR light (730nm) for 10 minutes) from day 6 and harvested L3 primordia or blade tissue at days 13, 16 and 20, or EoD FR from day 18, sampling at day 20. Plant kept under white light (WL) conditions were used as controls.
This RNA-seq was designed to help gain understanding on the genetic program behind phytochrome developmental time dependent control of leaf 3 (L3) cell proliferation and expansion phases, to ultimately regulate organ growth. Briefly, Arabidopsis thaliana Col-0 plants were grown under a light : dark (LD) 12 h : 12 h photoperiod, at a 100 µmoles/m2/s fluence rate and 21 °C of constant temperature. Plants were then exposed to either daily End of Day (EoD) far-red (FR, 40µmoles/m2/s of FR light (730nm) for 10 minutes) from day 6 and harvested L3 primordia or blade tissue at days 13, 16 and 20, or EoD FR from day 18, sampling at day 20. Plant kept under white light (WL) conditions were used as controls.