GSE185299
Gene expression profile of  human leukemic cell line treated with TLR1/2 agonist.
Treatment of MV4;11 cells with CU-T12-9 increased ubiquitination of c-Myc compared to control and correspondingly resulted in reduced K148 acetylation.  Chromatin immunoprecipitation coupled to high-throughput sequencing (Chip-Seq) revealed a significant loss of c-Myc occupancy in the genome following TLR1/2 activation with CU-T12-9.  As expected, diminished c-Myc binding resulted in gene downregulation of well-establish c-Myc targets.  These findings suggest that TLR-mediated K63-linked ubiquitination of c-Myc at K148 inhibits activation of c-Myc by interfering with its acetylation at the same residue. Overall design: mRNA profiles of MV4;11 cells treated with CU-T12-9.