ena-STUDY-UNIVERSITY OF BRITISH COLUMBIA-26-11-2018-15:08:25:735-1175
Animal host associated microeukaryotes
Unlike the study of bacterial microbiomes, the study of the microeukaryotes associated with animals has largely been restricted to visual identification or molecular targeting of particular groups. The application of high-throughput sequencing (HTS) approaches, such as those used to look at bacteria, has been restricted because the barcoding gene traditionally used to study microeukaryotic ecology and distribution in the environment, the Small Subunit of the Ribosomal RNA gene (18S rRNA), is also present in the animal host. As a result, when host-associated microbial eukaryotes are analyzed by HTS, the obtained reads tend to be dominated by host sequences. We have validated an easy, inexpensive, and near-universal method for the study of microeukaryotes associated with animal hosts using 18S rRNA Illumina metabarcoding. This method will contribute to a better understanding of microbial communities, as they related to the wellbeing of animals and humans.
Unlike the study of bacterial microbiomes, the study of the microeukaryotes associated with animals has largely been restricted to visual identification or molecular targeting of particular groups. The application of high-throughput sequencing (HTS) approaches, such as those used to look at bacteria, has been restricted because the barcoding gene traditionally used to study microeukaryotic ecology and distribution in the environment, the Small Subunit of the Ribosomal RNA gene (18S rRNA), is also present in the animal host. As a result, when host-associated microbial eukaryotes are analyzed by HTS, the obtained reads tend to be dominated by host sequences. We have validated an easy, inexpensive, and near-universal method for the study of microeukaryotes associated with animal hosts using 18S rRNA Illumina metabarcoding. This method will contribute to a better understanding of microbial communities, as they related to the wellbeing of animals and humans.