ena-STUDY-INSTITUT JACQUES MONOD-08-09-2020-22:04:45:517-719
Paramecium tetraurelia PacBio RSII sequencing T50 stage
Aims: Characterizing the distribution of 6mA (6-methyladenine) in Paramecium tetraurelia.  Materials & methods: Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument. 6mA were detected and mapped using the ipdSummary.py script from SMRT-v2.3.0.  Results: Our results revealed enrichment of 6mA into clusters separated by a periodic distance downstream Transcription Start Sites in the somatic genome.
Aims: Characterizing the distribution of 6mA (6-methyladenine) in Paramecium tetraurelia.  Materials & methods: Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument. 6mA were detected and mapped using the ipdSummary.py script from SMRT-v2.3.0.  Results: Our results revealed enrichment of 6mA into clusters separated by a periodic distance downstream Transcription Start Sites in the somatic genome.