GSE112880
Analysis of gene expression heteroegeneity in adult undifferentiated spermatogonia [single-cell RNA-Seq]
The undifferentiated spermatogonial population of mouse testis is functionally heterogeneous and contains stem cells and committed progenitor cells. However, gene expression patterns marking these distinct cell fractions are poorly defined. Therefore, our aim was to profile gene expression of undifferentiated cells by RNA-Seq at the single cell level. Undifferentiated cells were therefore isolated from adult mouse testes based on expression of a Plzf/Zbtb16 gene reporter, which marks both stem and progenitor spermatogonia. Isolated cells were then processed using the 10X Chromium system and analysed by RNA-Seq. Our goal was to characterize distinct subsets of undifferentiated spermatogonia based on gene expression patterns. Overall design: Undifferentiated spermatogonia were independently isolated from 2 adult Plzf-mCherry/CreER reporter adult mice and samples processed independently.