Cucumber seed associated bacterial community
Colonization of cucumber seeds by bacteria during germination
DNA samples were obtaiend by extraction from cucumber (Cucumis sativus c.v "Kfir") seed samples after 24 to 48 hours of germination. Bacterial 16S rRNA gene fragments were amplified, direclty from total DNA extracts, and sequenced by the 454 pyrosequencing method (using primer pair 27f and 507r).
Mass sequencing of bacterial 16S rRNA gene fragments was performed in order to assess bacteiral composition and community structure on germinating seeds of cucumber (Cucumis sativus). Seeds were germinated for 24-48h in perlite, bio-soilds compost-amended perlite and in the presence of the oomycete plant-pathogen Pythium aphanidermatum. analysis was done on total DNA extracted form the seed and seedling samples.