GSE114058
Transcriptional response of Ostreococcus tauri to Cu challenge
We test the transcriptional response of O. tauri RCC745 cells to two different copper concentration (0,4 and 2 µM), after 1, 4 and 12 h of copper amendments. Overall design: RNAseq libraries were constructed using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) by the NGS Service FASTERIS (Switzerland). Libraries were multiplexed in one channel of 1 x 50 bp run on a MiSeq run for titration and quality control. Finally, the libraries were sequenced using an Illumina HiSeq 2000 sequencer. All sequences were searched for homologies against the KEGG database and against the most recent O. tauri genome annotation.