PRJNA268374
Home sapiens HEK293 NCSTN knockout by Cas9
We sought to recapitulate the haploinsufficiency see in hidradenitis suppurativa famlies with cell culture. Cas9 was used to target NCSTN for knockout, and then an overlapping amplicon was amplified out and sequenced to show what deletion haplotypes were visible from the single-cell clones.