GSE104962
RNA-Sequencing for pancreatic cancer cells with scramble-shRNA and GPx1-shRNA
We reported glutathione peroxidase-1 (GPx1) was negatively associated with overall survival in pancreatic ductal adenocarcinoma patients. Silencing GPx1 in pancreatic cancer cells showed epithelial–mesenchymal transition phenotype and increased chemoresistance to gemcitabine in vitro and in vivo. Next, to search for a putative molecular mechanism, we used a high-throughput gene expression profiling array in scramble-shRNA and GPx1-shRNA pancreatic cancer cells (MiaPaCa-2). This study provides the differentially expressed genes and altered signaling pathways towards characterization of gemcitabine resistance cell populations. Overall design: The mRNA profiles of scramble-shRNA and GPx1-shRNA pancreatic cancer cells were generated by RNA-sequencing, in triplicate, using Illumina HiSeq 4000 Sequencing System.