PRJNA842514
Pancreatic and Ileal bacterial dysbiosis of acute pancreatitis in three mouse models
Acute pancreatitis (AP) is accompanied by gut microbiota dysbiosis, however, the composition of pancreatic microbiota associated with AP is still unknown. Methods: Caerulein (CAE), caerulein+lipopolysaccharide (CAE+LPS) and L-arginine (ARG) were used to induce AP in mice. Pancreas and ileum were collected for histological study and bacterial 16S rRNA gene sequencing. Blood were for analysis of amylase, lipase and inflammatory markers. Results: All the three modeling methods caused AP and ileal injury and inflammation. Rarefaction and Shannon indexes showed that AP mice had a more abundant microbial diversity in both pancreas and ileum. Principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis revealed microbial structural segregation between the AP and control groups, and between ARG and the two CAE groups (CAE, CAE+LPS) in the pancreas and ileum. Taxonomic analysis at the genus level and linear discriminant analysis effect size (LEfSe) at the operational taxonomic units (OTUs) level illustrated AP mice exhibited a marked increase in the relative abundance of Muribaculaceae and decrease in that of Dietzia both in pancreas and ileum, and reduce in Bifidobacterium only in the ileum; in addition, Roseburia was enriched in the two CAE groups in pancreas and/or ileum, while Escherichia-Shigella expanded in the pancreas of the ARG group. Spearman correlation analysis between pancreatic and ileal microbiota revealed that the abundance of Muribaculaceae, Dietzia and Roseburia in the pancreas were related to that in the ileum. Conclusions: These findings demonstrated that caerulein and L-arginine differentially disturbed the pancreatic and ileal microbiota when inducing AP.