GSE111574
H2A.Z is not involved in transcriptional regulation of MEF cells [RNA-Seq - MEFs]
We have studied how the absence of H2A.Z affects gene transcription in cell culture. For this purpose we have derived MEF cells from our mouse H2A.Z-1flox/flox x H2A.Z-2flox/flox lines and we have grown them until confluency to reach the G0 phase of the cell cycle. This allows to overcome the potential H2A.Z function during replication and cell division. Overall design: We study how the absence of H2A.Z affects gene transcription in cells in culture by using confluent, not dividing MEF cells. RNA-seq analysis was carried out from 3 biological replicates isolated from MEFs WT or KO for H2A.Z.