GSE171768
Single nucleus RNA sequence (snRNAseq) analysis of the spectrum of trophoblast lineages generated from human pluripotent stem cells in vitro
We report the single nuclear RNA sequencing analysis for the profiling of trophoblast lineages generated from human embryoinc stem cells (hESCs) in vitro under 20 % and 5 % O2 concentration conditions. The hESCs were exposed to bone morphogenetic protein 4 (BMP4) in presence of inhibitors of  ACTIVIN/TGFB (A83–01) and FGF2 (PD1730) signaling formerly called BAP treatment for 8 days after passaging, which  generated a mixture of cytotrophoblast, syncytiotrophoblast, and HLA-G positive cells with similarities to extravillous trophoblast under both high and low O2 conditions (20 % and 5 %, respectively). A single nuclei RNA sequence approach from both O2 conditions determined two major groupings of cell clusters, one comprised of five and the second of four subcluster cell populations respectively. Of these, two  subclusters resembled extravillous trophoblast, two carried the hallmark transcripts of syncytiotrophoblast, while the remaining five were likely different kinds of mononucleated cytotrophoblast. Overall design: Single nucleus RNA sequence (snRNAseq) analysis on Human embryonic stem cells (hESCs) converted trophoblast that are cultured under 20 % and 5 % O2 concentration conditions