GSE110729
Long non-coding RNAs associated with metabolic traits in human white adipose tissue
Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules  proposed to regulate various cellular processes. Here, we systematically analyzed their  expression in human subcutaneous white adipose tissue (WAT) and found that a  limited set was differentially expressed in either obesity or the insulin resistance that  usually follows obesity. Two lncRNAs herein termed adipocyte-specific metabolic  related lncRNAs 1(ASMER-1) and ASMER-2 were enriched in adipocytes and  regulated by both obesity and insulin resistance. Knockdown of either ASMER-1or  ASMER-2 by antisense oligonucleotides using in vitro differentiated human adipocytes  altered lipolysis and adiponectin secretion, respectively. The observed effects could be  attributed to changes in the expression of genes controlling inflammation and lipid  metabolism. Our data demonstrate that lncRNAs are modulators of the metabolic and  endocrine functions in fat cells and also provide an emerging link between WAT and  common metabolic conditions. Overall design: Human adipose tissues mRNA profiles of obese (13) and lean (15) subjects were generated by deep sequencing using Illumina 2000.