PRJNA578479
RNA-seq raw data of five developmental pod stages in two accessions of common bean differing in several relevant agronomic traits
With the aim to identify genes involved specifically in common bean pod maturation process, an RNA-seq analysis has been carried out in five developmental pod stages, from fruit setting to maturation, and in two accessions of common bean differing in several relevant agronomic traits. Results of this RNA-Seq analysis provide valuable information for understanding the transcriptional regulation of pod maturation in common bean.Overall design: Three biological replicates for each genotype and developmental stage were sequenced, each one with six uniformly sized pods. Common bean plants were grown in a growth chamber (20-25 °C, relative humidity 70-90%, 8/16 h light/dark regime). Standard management practices were used including regular addition of fertilizers. Total RNA was isolated using TriFast Reagent (Peqlab, Erlangen, Germany) following the manufacturer's instructions. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The cDNA library insert sizes ranged from 250 to 300 bp. The complete libraries were purified with AMPure XP system (Beckman Coulter, Beverly, MA, USA) and qualified by Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Finally, libraries were sequenced on an Illumina Hiseq 2500 platform (Illumina Inc., San Diego, CA, USA), and 150 bp paired-end reads were generated.