PRJNA380492
Halomonas alkalicola  Genome sequencing and assembly
A Gram-stain-negative, alkaliphilic and moderately halophilic bacterium, designated as 56-L4-10aEnT, was isolated from a household product plant in China. Cells of the novel isolate were rod-shaped, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belongs to the genus Halomonas, to which six closest neighbors being Halomonas mongoliensis Z-7009T (97.59%), Halomonas ventosae Al12T (97.35%), Halomonas campaniensis 5AGT (97.22%), Halomonas alimentaria YKJ-16T (97.22%), Halomonas shengliensis SL014B-85T (97.12%) and Halomonas fontilapidosi 5CRT (97.09%). The main polar lipids of strain 56-L4-10aEnT contained diphosphatidyl glycerol, phosphatidyl glycerol and phosphatidyl ethanolamine. The predominant respiratory quinone was Q-9, with Q-8 as a minor component. The major fatty acids were C18:1 ?7c/C18:1 ?6c and C16:0. Strain 56-L4-10aEnT was clearly distinguished from those type strains mentioned above through phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data analysis and a range of physiological and biochemical characteristics comparisons. It is evident from the genotypic and phenotypic data that strain 56-L4-10aEnT could be classified as a novel species of the genus Halomonas, for which the name Halomonas alkalicola sp. nov. is proposed. The type strain is 56-L4-10aEnT (= CICC 11012sT= DSM 103354T).