GSE109315
Gene expression analysis of mouse brain regions after chronic social defeat stress (CSDS)
Chronic psychosocial stress is a risk factor for psychiatric disorders, and genetic factors interact in conferring susceptibility. The chronic social defeat stress (CSDS) mouse model allows identifying factors underlying resilience and susceptibility to chronic psychosocial stress. We used RNA-sequencing to identify genes and pathways affected by CSDS in three brain regions: medial prefrontal cortex (mPFC), ventral hippocampus (vHPC), and bed nucleus of the stria terminalis (BNST), of male mice from strains C57BL/6Crl and DBA/2Crl. Overall design: Ribo-depleted RNA was sequenced with Illumina High-Seq 2000 and Next-seq 500 from mPFC (C57BL/6Crl: control n = 6, resilient n = 6, susceptible n = 6; DBA/2Crl: control n = 6, susceptible n = 8), BNST (C57BL/6Crl: control n = 5, resilient n = 5, susceptible n = 5; DBA/2Crl: control n = 5, resilient n = 3, susceptible n = 5), and vHPC (C57BL/6Crl: control n = 6, resilient n = 8, susceptible n = 3; DBA/2Crl: control n = 6, susceptible n = 5) 6-8 days after the last CSDS.