E-MTAB-7203
RNA-seq of med14-3 and uvh6-3 mutants in heat stress (37°C) and control stress (23°C), with WT controls. RNA-seq of double mutants: med14-3 or uvh6-3 combined with either ddm1-2 or mom1-2 mutants. Bisulfite-seq of WT samples subjected to control or heat stress. Bisulfite-seq of med14-3 and WT samples in control stress.
Heterochromatin transcription is functionally important, for instance to establish heterochromatin silencing or condensation, but the factors allowing transcription in a repressive chromatin environment are little known. To gain insight in this process, we triggered heterochromatin transcription using heat stress or mutations for the silencing factors DDM1 or MOM1 and generated mRNA profiles of med14 and uvh6 mutants in these different contexts. We used rosette leaves incubated 24h in dH2O at 23°C for control stress and 37°C for heat stress. Our data and analysis suggest that UVH6/XPD/RAD3 is involved in a heat stress specific transcriptional process where it promotes transcription of most but not all genes. MED14 promotes transcription at a more reduced number of loci during heat stress, and preferentially targets heterochromatic loci. In addition, MED14 is required for heterochromatin transcription without heat stress, but this function is lost when heterochromatin properties are disrupted, suggesting it is specifically involved in transcription of heterochromatic sequences. In addition, bisulfite-seq of med14 mutants indicates that MED14 participates in RNA-directed DNA methylation, suggesting that MED14 is required both for heterochromatin transcription and formation.
Heterochromatin transcription is functionally important, for instance to establish heterochromatin silencing or condensation, but the factors allowing transcription in a repressive chromatin environment are little known. To gain insight in this process, we triggered heterochromatin transcription using heat stress or mutations for the silencing factors DDM1 or MOM1 and generated mRNA profiles of med14 and uvh6 mutants in these different contexts. We used rosette leaves incubated 24h in dH2O at 23°C for control stress and 37°C for heat stress. Our data and analysis suggest that UVH6/XPD/RAD3 is involved in a heat stress specific transcriptional process where it promotes transcription of most but not all genes. MED14 promotes transcription at a more reduced number of loci during heat stress, and preferentially targets heterochromatic loci. In addition, MED14 is required for heterochromatin transcription without heat stress, but this function is lost when heterochromatin properties are disrupted, suggesting it is specifically involved in transcription of heterochromatic sequences. In addition, bisulfite-seq of med14 mutants indicates that MED14 participates in RNA-directed DNA methylation, suggesting that MED14 is required both for heterochromatin transcription and formation.