GSE48717
GSE48717: Identification of Transcription Factor LSY-2::GFP Binding Regions in Starved
Summary: modENCODE_submission_3846 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals.  Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: DevStageWorm:starved L1:MS:1(official name : starved L1 ); Developmental Stage: L1 larva; Genotype: unc-119(ed3); wgIs367(lsy-2::TY1 EGFP FLAG; unc-119); Sex: Hermaphrodite;  EXPERIMENTAL FACTORS: Developmental Stage L1 larva; Target gene lsy-2; Strain DevStageWorm:starved L1:MS:1(official name : starved L1 ); temp (temperature) 20 degree celsius