GSE172393
Gene expression profiles of HSCs from P0 livers.
Purpose: The study was designed to identify transcriptional differences of P0 liver HSCs with different genotypes or different cell size. Method: For RNA-seq, libraries were prepared according to the Smart-seq2 protocol from 100-200 sorted HSCs. Samples were sequenced by NextSeq500 (Illumina) with single-end 75-bp read length using the NextSeq 500/550 High Output v2 Kit (75 cycles, Illumina). The RNA-seq pipeline from Basepair (www.basepairtech.com) was used for the analysis. Expression count was analyzed by STAR and differential expression by DESeq2 (P < 0.05 and fold-change > 2). Overall design: HSC mRNA profiles from P0 livers.