GSE104596
Transcriptome analysis of Gibberellin-response gene in the fast-growing species moso Bamboo
Moso bamboo is well known with its rapid growth rate. In order to shed light on the underlying mechanism of growth, we applied Gibberellin (GA) to mimic the fast development process in bamboo and found that exogenous GA can significantly increase the length of internode of moso bamboo. Subsequently, we sequenced the transcriptome by high-through sequencing and identified 5,148 different expressed genes (DEG) and find out 22 genes that take part in GA signaling pathway is significantly differently expressed, including GID1A, GID1B and GAI. From GO analysis, a group of genes related to response hormone stimulus are enriched in up-regulated DEGs and 178 GA-responded genes are involved in other plant hormone signaling pathway, of which genes MYB74 and NCED5 are belong to both two pathways. Besides, clusters of genes about photosynthesis are enriched in down-regulated DEGs. By looking into the content of chlorophyll, we find out that the concentrations of chlorophyll are obviously decreased which verify that photosynthesis do weakened after GA treatment. Through method of slicing by hand, it appears that an increased condensation of lignin after GA treatment. The results imply a functional role of GA in bamboo growth, and maybe major in cell wall formation, and photosynthesis. Differential AS alternative splicing (AS) events upons GA induce suggested that post-transcriptional regulation is involved in the GA response. Overall design: triplicates