PRJNA270670
Arabidopsis thaliana cultivar:Col-0 Transcriptome or Gene expression
Using an adapted version of nanoCAGE (nanoCAGE-2000) for sequencing on the Illumina HiSeq-2000, we sequenced the 5'' ends of gene transcripts in Arabidopsis thaliana. The protocol targets the 5'' end of transcripts by utilizing the cap-dependent template switching properties of reverse transcriptase, thereby capturing the transcriptional start sites (TSS) of RNA polymerase II genes. The end-goal being improved coverage of the TSSs while leveraging the lower RNA input requirements afforded by the nanoCAGE protocol.