ena-STUDY-UFRJ-12-04-2018-15:45:05:575-43
We are studying the expression of several hydrolases and oxidases of digestive system of Cornitermes cumulans
Nest fragments of three C. cumulans mounds were collected from Pinheiral grasslands, Rio de Janeiro, Brazil (GPS data: -22.5212761 and -43.9990613) and kept in polypropylene containers in a dark laboratory, at 23 ± 2°C and 60 ± 10% relative humidity. The experiments were performed using worker caste termites of undetermined age and with full guts.Total RNA was extracted using Trizol® reagent (Ambion™) following the manufacturer's standard protocol, precipitated with 1.4 M NaCl and re-purified using the RNeasy Mini kit (Qiagen) according to the manufacturer's recommendations. RNA was subjected to RNase-free DNase I (Invitrogen™) treatment to remove any potential DNA contamination, and complementary DNA (cDNA) was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).
Nest fragments of three C. cumulans mounds were collected from Pinheiral grasslands, Rio de Janeiro, Brazil (GPS data: -22.5212761 and -43.9990613) and kept in polypropylene containers in a dark laboratory, at 23 ± 2°C and 60 ± 10% relative humidity. The experiments were performed using worker caste termites of undetermined age and with full guts.Total RNA was extracted using Trizol® reagent (Ambion™) following the manufacturer's standard protocol, precipitated with 1.4 M NaCl and re-purified using the RNeasy Mini kit (Qiagen) according to the manufacturer's recommendations. RNA was subjected to RNase-free DNase I (Invitrogen™) treatment to remove any potential DNA contamination, and complementary DNA (cDNA) was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).