GSE193912
Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events
The goal is to compare role of Not1,4,5 in co-translational decay and solubility of RNAs from soluble and total RNA pools using degron strains Overall design: The cells are grown exponentially and total RNA is prepared by acid phenol extraction from cell pellets or from cell lysates prepared as for polysome profiling