GSE115337
NKX3.1 localization to mitochondria suppresses prostate cancer initiation  (Mouse 4M Prostate RNASeq)
The NKX3.1 homeobox gene functions in mitochondria to regulate oxidative stress To investigate the role of NKX3.1 in regulation of oxidative stress, we employed transcriptome analysis of mouse prostate ( from 4 month old Nkx3.1+/+ and Nkx3.1-/- mice treated with paraquat), and human prostate cells ( RWPE1 cells engineered with empty vector (altered pTRIPZ), NKX3.1 wild type over-expression, and NKX3.1 (R52C) over-expression treated with paraquat). Mouse tissue or human cells were snap frozen for subsequent molecular analysis. Overall design: Analysis of transcriptome of prostate tissue from 4 month old mouse tissue Prostate tissue from 4 month old Nkx3.1+/+ and Nkx3.1-/- mice  treated with paraquat was harvested, and snap frozen for subsequent molecular analysis Total RNA obtained from prostate tissues. Prostate tissues were harvested and processed for RNA isolation and transcriptome analysis using the MagMAX RNA isolation kit (Ambion). For queries, please contact Cory Abate-Shen at cabateshen@columbia.edu