ena-STUDY-Teagasc Crop Science-18-02-2019-11:21:04:146-651
Whole genome resequencing of five pools of 15 tetraploid potato cultivars
In this study, we present an approach to consolidate various types of molecular markers to a single SNP-based platform using whole genome sequencing of carefully constructed pools of DNA using, as examples, molecular markers for the H1 nematode and R2 blight resistance genes. The approach led to the identification of a number of candidate nucleotide polymorphisms which allowed successful design and deployment of Kompetitive Allele Specific PCR (KASP) assays. We further show how the sequence data generated can be used to aid design of KASP assays for diagnostic SNPs for other traits mined from the literature.
In this study, we present an approach to consolidate various types of molecular markers to a single SNP-based platform using whole genome sequencing of carefully constructed pools of DNA using, as examples, molecular markers for the H1 nematode and R2 blight resistance genes. The approach led to the identification of a number of candidate nucleotide polymorphisms which allowed successful design and deployment of Kompetitive Allele Specific PCR (KASP) assays. We further show how the sequence data generated can be used to aid design of KASP assays for diagnostic SNPs for other traits mined from the literature.