PRJNA282369
Macrotyloma uniflorum cultivar:PI277736 Transcriptome or Gene expression
15 days old seeds Macrotyloma uniflorum were subjected to 150mM salt and 15% PEG induced drought stress for 12h. RNA was isolated from leaf and root tissues.cDNA libraries were constructed and sequenced using Illumina HiSeq 2500 Platform to generate 100 bp PE reads.Quality of reads were assessed and de novo transcriptome assembly was performed using Trinity. The reads were annotated and statistical analysis including count normalization and differential expression was performed with edgeR. Differentially expressed genes under stress conditions were analyzed further.