GSE208094
RNA-seq analysis of transcriptomes of WT and Foxp3?2 Treg cells in spleen and LNs
We performed RNA-seq analysis of Tregs isolated from Foxp3RFP/?2Foxp3?CNS1-cre-Thy1.1Rosa26LSL-YFP female mice. CD4+YFP+RFP+ (WT Tregs) and CD4+YFP+RFP- (Foxp3?2 Tregs) cells were sorted from lymph nodes and spleen to a typical purity of >95% We detected 638 differentially expressed genes (DEGs, FDR < 0.05) between Foxp3?2 and WT Tregs Overall design: Transcriptional profiles of WT and Foxp3?2 Treg cells in spleen and LNs