GSE119512
Effects of Bloom RNAi on Transcriptomes in A549 Cells
We profiled transcriptomes in human lung cancer cell line A549 when the expression of Bloom was knockdown by the siRNA specific to Bloom. Overall design: A549 cells were cultured in DMEM.  Bloom-specific and control siRNAs were transfected into A549 cells using RNAiMAX. Total cell RNA was extracted at 48 hrs after transfection. Three independent transfections were carried out for each siRNA. The extracted RNA was processed for RNA-SEQ on Illumina HIGHSEQ 2500.