GSE126575
VenomSeq - 25 venoms vs. IMR-32
We report the perturbational effects of 25 crude venoms on human IMR-32 (neuroblastoma) cells. These data are used to evaluate the VenomSeq transcriptomic data analysis workflow, and to provide the first dataset generated by this workflow. By comparison with data published by the Connectivity Map team, and other free sources of genetic perturbation in the context of disease or drug exposure, we use these data to discover several novel associations between venoms and specific therapeutic actions. Overall design: 192 samples total, corresponding to 2 96-well plates of samples. These 192 samples are treated with each of 25 venoms, where extraction takes place at each of 3 time points with 2 replicates per time point (6 samples per venom). 24  samples were treated with water only, and 18 were left untreated. Dosages per venom were determined experimentally as the concentration at which cell growth was inhibited by 20%.