GSE132588
Log-term effects of proton irradiation on DNA methylation and metabolomic pathways in the hippocampus and heart.
The goal of this experiment was to identify methylation and metabolomic pathways the respond to proton irradiation and examine whether these two pathways coregulate biological pathways. Overall design: DNA was isolated 20 weeks after 0 Gy (sham) or 1 Gy Proton irradiation.  1 ug of hippocamal or left ventricular DNA from 5 mice in each treatment group was pooled, resulting in two pooled biological replicates for each treatment condition. Pooled DNA was subjected to 5hmC DIP and Illumina TrueSeq libraries were constructed.