GSE118474
Insights into transcriptional characteristics and homoeolog expression bias of embryo and endosperm in developing grain through mRNA-Seq and Iso-Seq
To analyse the regulation of transcripts in grain embryo and endosperm during  development, we performed RNA-Seq for wheat from 14 and 25 day post anthesis (DPA). And long-read sequencing for mixed whole grains from 14 and 25 DPA was employed to obtain full-length transcripts. A series of differentially expressed genes and tissues-specific genes of embryo and endosperm were identified. Moreover, 4351, 4641, 4516 and 4453 genes with A, B and D homoeoloci were detected in the four tissues. These provide specific gene pools of embryo and endosperm and homoeolog expression bias model in a large scale, which provides new insights into the molecular physiology of wheat. Overall design: Wheat (Triticum aestivum L.) cultivar Zhou 8425B were field cultivated. Embryo and endosperm (including episperm and pericarp) at 14 and 25 day post anthesis (DPA) were collected respectively for three biological replicates and immediately frozen in liquid nitrogen. They were then stored at -80°C following RNA extraction. Totals RNAs were extracted from each tissue sample using Plant Total RNA Purification Kit (GMbiolab co., Ltd. Taichung, Taiwan) according to the manufacturer's protocol. Finally, the twelve RNA librarie sequencing based on Illumina HiSeq 4000 platform by paired-end experiments.