ena-STUDY-THE CHINESE UNIVERSITY OF HONG KONG-19-01-2018-07:32:34:887-963
In this study, tumor xenografts, cancer cell lines and their corresponding normal samples were fully characterized using whole-genome sequencing.
The lack of representative nasopharyngeal carcinoma (NPC) models has seriously hampered research on EBV carcinogenesis and preclinical studies in NPC. Fifty-eight NPC specimens were transplanted in immunodeficient mice to initiate growth of patient-derived xenografts (PDXs). Five NPC PDXs were successfully generated. The take rates for primary and recurrent NPC were 4.9% and 17.6%, respectively. The suppression of lytic reactivation of EBV by the Rho-associated coiled-coil containing kinases inhibitor (Y27632) in vitro resulted in the successful establishment of a new EBV-positive NPC cell line (NPC43). Spontaneous lytic reactivation of EBV and production of infectious EBV were observed in NPC43 upon withdrawal of Y27632. Whole-exome sequencing (WES) revealed similar mutational profiles of these PDXs with their corresponding patient NPC. Whole-genome sequencing (WGS) delineated the genomic landscape and sequences of EBV genomes in these NPC models. The genomic profiles of these models support their uses for future studies in NPC.
The lack of representative nasopharyngeal carcinoma (NPC) models has seriously hampered research on EBV carcinogenesis and preclinical studies in NPC. Fifty-eight NPC specimens were transplanted in immunodeficient mice to initiate growth of patient-derived xenografts (PDXs). Five NPC PDXs were successfully generated. The take rates for primary and recurrent NPC were 4.9% and 17.6%, respectively. The suppression of lytic reactivation of EBV by the Rho-associated coiled-coil containing kinases inhibitor (Y27632) in vitro resulted in the successful establishment of a new EBV-positive NPC cell line (NPC43). Spontaneous lytic reactivation of EBV and production of infectious EBV were observed in NPC43 upon withdrawal of Y27632. Whole-exome sequencing (WES) revealed similar mutational profiles of these PDXs with their corresponding patient NPC. Whole-genome sequencing (WGS) delineated the genomic landscape and sequences of EBV genomes in these NPC models. The genomic profiles of these models support their uses for future studies in NPC.