DRP007046
Generation of de novo telomere end by CRISPR-based large-scale genome editing
We developed the novel CRISPR/Cas9 system in the rodent malaria parasites Plasmodium berghei. We conducted the large-scale genome editing to delete the subtelomeric regions using a donor template DNA with telomere sequences. The combination of Illumina sequencing and Nanopore-based long read sequencing revealed the deletion of subtelomeric regions and extension of telomere seeding sequences.