GSE114281
Growth enhancement of obligate anaerobic archaeon, Thermococcus onnurineus NA1 by the addition of oxygen
Hyperthermophilic archeaon, Thermococcus onnurineus NA1 has known as a strict anaerobe. To date, a few of studies have been reported that strict anaerobe can grow using oxygen. However, the research of the growth enhancement of strict anaerobic archaeon belonging to the order of Thermococcales using the oxygen, in which has never been reported so far. In this study, we showed that the growth of T. onnurineus NA1 strain increased under various oxygen concentrations and we observed that oxygen was decreased in the headspace during the growth of cell. Genome-wide transcriptomic analysis was carried out to evaluate alterations in gene expression induced by O2 and to explain the physiological effects of oxidative stress on the growth of T. onnurineus NA1. Overall design: RNA expreesion profile of T. onnurineus NA1 genes in the absence or presence of 20 % O2-containing media, using Illumina HiSeq 2500