Repair_profiles_in_DNA_repair_mutant_mouse_ES_cells_-sc-6112
Repair_profiles_in_DNA_repair_mutant_mouse_ES_cells_
We have developed a CRISPR/Cas9 platform for the analysis of DNA repair outcomes in mouse ES cells. This is a pilot experiment aimed to verify that our current protocol can be scaled up effectively, for future upscaling. Mouse embryonic stem cells that express Cas9 and have a mutation in a DNA repair pathway gene are infected with lentivirus that encodes for both a guide RNA and a target DNA sequence for that guide. After screening the cells for 2 weeks the genomic DNA is extracted and the target DNA is amplified by PCR and sequenced.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We have developed a CRISPR/Cas9 platform for the analysis of DNA repair outcomes in mouse ES cells. This is a pilot experiment aimed to verify that our current protocol can be scaled up effectively, for future upscaling. It is important to know that protocol upscaling does not affect the quality of the results.