E-MTAB-10996
RNA-seq of control and CHH (cartilage-hair hypoplasia) fibroblasts transdifferentiated into chondrocytes-like cells
We used primary human CHH (cartilage-hair hypoplasia) and control fibroblasts in a chondrogenic transdifferentiation model (FDC; fibroblast-derived chondrocytes) to determine the chondrogenic capacity and differential pathway regulation of CHH cells. For the sequencing experiment, dermal fibroblasts from control donors (n=4) and CHH patients (n=4) were isolated from skin biopsies, plated at high density into wells coated with aggrecan, and cultured for three days in an FDC transdifferentiation medium. Total RNA was isolated at three different time points (Day 0, 1, and 3; in total 24 RNA samples), and sequenced using the NextSeq platform (Illumina).
We used primary human CHH (cartilage-hair hypoplasia) and control fibroblasts in a chondrogenic transdifferentiation model (FDC; fibroblast-derived chondrocytes) to determine the chondrogenic capacity and differential pathway regulation of CHH cells. For the sequencing experiment, dermal fibroblasts from control donors (n=4) and CHH patients (n=4) were isolated from skin biopsies, plated at high density into wells coated with aggrecan, and cultured for three days in an FDC transdifferentiation medium. Total RNA was isolated at three different time points (Day 0, 1, and 3; in total 24 RNA samples), and sequenced using the NextSeq platform (Illumina).