E-MTAB-2488
4C analysis to capture chromatin conformations of the Tfap2c-Bmp7 locus upon different genomic rearrangements in mice
A genomic locus tends to form a particular conformational structure in the nucleus, but it is not known how a specific structure is dictated. To understand the mechanism, we made serial genomic rearrangements (deletion and inversion) in the Tfap2c-Bmp7 locus in mice, and analyzed the chromatin conformation in these alleles by 4C. For the wild type allele, we took not only the whole embryo but also different tissues such as the heart, forebrain (lateral and medial part separately), limb buds, and we used only the whole embryos for the mutant alleles.
A genomic locus tends to form a particular conformational structure in the nucleus, but it is not known how a specific structure is dictated. To understand the mechanism, we made serial genomic rearrangements (deletion and inversion) in the Tfap2c-Bmp7 locus in mice, and analyzed the chromatin conformation in these alleles by 4C. For the wild type allele, we took not only the whole embryo but also different tissues such as the heart, forebrain (lateral and medial part separately), limb buds, and we used only the whole embryos for the mutant alleles.