GSE162382
The chromatin regulator JMJD3 partially mediates the polyamine-dependent effect on Th17 cells
We studied the effects of polyamine pathway inhibitors on differentiation of Th17 cells in vitro. Here, we used difluoromethylornithine (DFMO), an irreversible inhibitor of ODC1, the enzyme that catalyzes the conversion of ornithine to putrescine, against a genetic background of WT or Jmjd3-deficient cells. Overall design: We analyzed the effect of DFMO on Th17 cells differentiated from naïve CD4 T cells isolated from control or JMJD3fl/flCD4cre mice