PRJNA721582
SARS-CoV-2/human/Marburg/PUM-D2417/2021
Patient isolate D2417 was cultivated and first passaged on VeroE6 TMPRSS2 cells, then on Clau3 cells and again on VeroE76 TMPRSS2 cells. In total 3 Passages. Virus was concentrated by ultracentrifugation, RNA was isolated using RNeasy (Qiagen) Kit.