GSE138224
Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy
High-throughput RNA-seq analysis was performed using fresh residual CT26 tumors 3 days after incomplete radiofrequency abaltion (iRFA) as well as control tumors.   We ound genes encoding damage-associated molecular patterns molecules (DAMPs), proinflammatory cytokines, chemokines, and genes associated with immunosuppression were overexpressed in the iRFA-treated tumor, indicating iRFA  elicits inflammation and induces immunosuppression in residual tumors. Overall design: Tumor mRNA profiles of CT26 colon cancer in Balc/c mice in two different groups (control and iRFA ) were generated by deep sequencing, in triplicate.