GSE168637
Joint single-cell multiomic analysis identifies Aebp2 as a key regulator in Wnt3a induced asymmetric stem cell division
Wnt signal usually functions as a spatial gradient. A localized Wnt3a can induce asymmetric division of mouse embryonic stem cells, whereas proximal daughter cells maintain self-renew and distal daughter cells acquire hallmarks of differentiation. Here, we develop an approach, SET-seq (same cell epigenome and transcriptome sequencing), to jointly profile epigenome and transcriptome in the same single cell. By utilizing SET-seq, we profile H3K27me3 and H3K4me3 with gene expression in Wnt3a induced asymmetric cell division. H3K27me3, but not H3K4me3, is correlatedly changed with gene expression. Single cells clustered by H3K27me3 recapitulate the clusters defined by gene expression. Furthermore, Aebp2, which is the regulator of H3K27me3, is important for the cell-fate decision with localized Wnt signal. Our results provide a convenient way to jointly profile epigenome and transcriptome in the same cell, and uncover the mechanistic insights into the gene regulatory programs for maintaining and resetting stem cell fate during differentiation. Overall design: Single-cell level epigenomic and transcriptional profile in asymmetric division mESCs