GSE126837
A signature protein of Mycobacterium tuberculosis (Rv000) modulates the transcriptome of Mycobacterium smegmatis.
The world's second deadliest disease tuberculosis is caused by the organism Mycobacterium tuberculosis. Mtb has 4173 genes (Mycobrowser) and some of these genes are essential for its survival. There are many uncharacterized genes in Mtb which play an important role in its survival strategies to combat the immune system of the host. In order to identify vital genes required for its survival were characterized by in silico approach. Based on computational analysis, we have identified 2 proteins which are only present in Mtb complex and named as Signature Protein 1 & 2. The Signature protein 2 (Rv000) has DNA methyltransferase activity and also protects the DNA from DNase-I digestion. Knock in of this gene in Mycobacterium smegmatis modulates its transcriptome and thereby enhances the survival. RNA seq analysis of M. smegmatis showed that more than 3000 genes were up regulated at the transcriptional level. These results suggested that the gene encoding signature protein 2 (Rv000) is acting as a global transcriptional regulators. These genes encode proteins that belong to different categories of biological and molecular function i.e. transcriptional regulators, regulatory proteins, conserved hypothetical proteins, transporter proteins (ABC transporters, antiporter, sugar transporter and Major facilitator superfamily genes) and TonB dependent receptors (required for iron scavenging) were upregulated. The proteins encoded by these genes may be targeted for drug development against Mtb.. Overall design: 2 Wild Mycobacterium smegmatis, 2 recombinant mycobacterium smegmatis grown under normal conditions