GSE118595
The transcription factor Foxp1 preserves integrity of an active Foxp3 locus in extrathymic Treg cells
In order to identify if there is any role of Foxp1 in the DNA demethylation of Foxp3 enhancer regions of induced Treg (iTreg) cells we performed bisulfite sequence analysis of Foxp1 deficient in vitro generated iTreg cells and compared their methylation status after 7 days of iTreg induction, at which point Foxp1 is deleted in the iTreg cells derived from Foxp3YFPCreFoxp1f/f  (KO) mice. Overall design: Examination of methylation in 3 loci on the X chromosome by bisulfite sequencing protocol using in vitro generated itreg cells.