Transcriptome_profiling_of_mutants_from_the_zebrafish_mutation_project-sc-3313
Transcriptome_profiling_of_mutants_from_the_zebrafish_mutation_project
Total RNA was extracted from zebrafish embryos collected for one or more alleles identified by the Zebrafish Mutation Project (http://www.sanger.ac.uk/Projects/D_rerio/zmp/). The mRNA was pulled down using polyT oligos attached to magnetic beads and Illumina libraries were prepared using TruSeq Stranded mRNA SamplePreparation Kits (RS-122-2101 and RS-122-2102) according to manufacturer's instructions. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Paired-end sequence data from the Illumina HiSeq was prepared for zebrafish embryos collected for one or more alleles identified by the Zebrafish Mutation Project for transcriptome profiling.