BLCLs_3999_TSS
Massive transcriptional start site mapping of BLCLs_3999 cells.
Although recent studies have revealed that the majority of human genes             are subjected to regulation of alternative promoters (APs), the biological relevance of             this phenomenon remains unclear. To enable more comprehensive TSS analysis in the             respective cell types, we recently developed a method, combining oligo-capping with the             massively paralleled sequencing technology, Illumina GA. In this method, which we named             TSS Seq, sequence adaptor which is necessary for Illumina GA sequencing is directly             introduced to the cap site of the mRNA. By sequencing 36-48 sequence immediately             downstream of the TSSs (TSS tags), it is possible to obtain precise positional             information of transcriptional start sites (TSSs). In this paper, we used the TSS tag             data accumulated from twelve different cell types and normal tissues in humans for the             identification and characterization of the APs in human genes.
Comprehensive identification and characterization of the transcriptional             start sites of human genes were carried out. For this purpose, we used our TSS-Seq             method, in which next gene sequencing technology and our full-length cDNA library             technology, oligo-capping were combined.