Kangwha tidal flat populations regarding reductive dechlorination
Characterization of Microbial Community Structure and Population Dynamics of Tetrachloroethene-Dechlorinating Tidal Mudflat Communities using a Multipronged Approach
Microcosm studies and Titanium pyrosequencing characterized reductive dechlorinating activities and populations in tidal flat sediments collected from South Korea’s central west coast near Kangwha. In microcosms established with surface sediments, PCE dechlorination to TCE began within 10 days and 100% of the initial amount of PCE was converted to TCE after 37 days. cis-1,2-dichloroethene (cis-DCE) was observed as dechlorination end product in microcosms containing sediments collected from deeper zones (i.e., 35-40 cm below ground surface). Pyrosequencing of bacterial 16S rRNA genes revealed Desulfuromonas michiganensis-like populations predominanted in both TCE and cis-DCE producing microcosms. Other abundant groups included Desulfuromonas thiophila and Pelobacter acidigallici–like populations in the surface sediment microcosms, and Desulfovibrio dechloracetivorans and Fusibacter paucivorans–like populations in the deeper sediment microcosms. Dehalococcoides spp. populations were undetected in these sediments before and after incubation with PCE.
A multipronged approach comprising microcosm studies, Titanium pyrosequencing, 16S rRNA gene clone libraries, and dechlorinator-targeted quantitative real-time PCR (qPCR) characterized reductive dechlorinating activities and populations in tidal flat sediments collected from South Korea’s central west coast near Kangwha.