GSE200542
Ectopic expression of long noncoding RNA AI662270 led to differential gene expression in mouse mesangial cell line SV40 MES13
We generated the SV40 MES13 subline stably expressing long noncoding RNA AI662270 and performed transcriptome RNA sequence. Overall design: Total RNA was extracted from SV40 MES13 subline cells with TRIzol reagent. The RNA purity and integrity were analyzed with the Agilent Bioanalyzer 2100 (Agilent). The mRNA was purified from total RNA after removal of rRNA and sequenced on an Illumina Hiseq2000 system.