GSE120848
Isolation and genome-wide characterization of cellular DNA:RNA triplex structures III
RNA can directly bind to purine-rich DNA via Hoogsteen base pairing, forming a DNA:RNA triple helical structure that anchors the RNA to specific sequences and allows guiding of transcription regulators to distinct genomic loci. To unravel the prevalence of DNA:RNA triplexes in living cells, we have established a fast and cost-effective method that allows genome-wide mapping of DNA:RNA triplex interactions. In contrast to previous approaches applied for the identification of chromatin-associated RNAs, this method uses protein-free nucleic acids isolated from chromatin. High-throughput sequencing and computational analysis of DNA-associated RNA revealed a large set of RNAs which originate from non-coding and coding loci, including repeat elements. Combined analysis of DNA-associated RNA and RNA-associated DNA identified genomic DNA:RNA triplex structures. The results suggest that triplex formation is a general mechanism of RNA-mediated target-site recognition, which has major impact on biological functions. Overall design: We have established a ligation-mediated RNA pulldown method (TriplexDNA-seq) to enrich DNA regions that are directly associated with RNA. DNA from biological quadruplicates was sequenced (rep1-4_plus_RNA_ligation), samples omitting ligation serving as control (rep1-4_minus_RNA_ligation).