PRJNA532443
Corn Plants Root microbiome
Soil microbial community associated to Zea mays roots. 16S rRNA amplicon sequencing of corn root attached soil.Zea mays plants were grown in 2 L pots during 60 days, soil attached to the roots was processed in mortar for DNA extraction. DNA extraction were made with PowerSoil DNA isolation Kit (MoBio) following manufacturer's instructions.The V1-V3 hyper-variable region of the 16S rRNA gene was amplified by PCR using 27F (AGRGTTTGATCMTGGCTCAG) y 519R (GWATTACCGCGGCKGCTG) primers. Amplicon sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a Illumina MiSeq following the manufacturer's guidelines.