PRJNA479582
Necrotizing soft tissue infection tissue biopsy metagenome and metatranscriptome raw sequence reads
The aim of the study was to investigate the interactions of mono- and polymicrobial communities and the human host in necrotizing soft tissue infections (NSTI). Nested within the INFECT study we combined 16S rDNA and metatranscriptomic sequencing of NSTI tissue biopsies. DNA and RNA were extracted from tissue biopsy samples. Amplicon libraries of the 16S rRNA gene were generated and sequenced on the Illumina MiSeq platform. Metatranscriptome libraries were prepared from DNase-treated and rRNA-depleted RNA and sequenced on the Illumina HiSeq2500 platform.