PRJNA545820
Barcoding and demultiplexing Oxford Nanopore direct RNA sequencing reads using deep learning
We describe a novel strategy to barcode and demultiplex direct RNA sequencing, involving custom DNA oligonucleotides ligated to RNA transcripts during library preparation. The raw signal associated with the DNA barcode is extracted, transformed to an array of pixels, and demultiplexed using a deep convolutional neural network classifier