PRJNA721515
Additional HiC Data of TAD Shaking Induced by Master Regulator Controls Cell Fate
4D nucleome (4DN) plays important role in cell fate transition. However, the mechanism how master regulators control cell fate through 4DN, and what outcome can be obtained from the perspective of 4DN to guide cell fate transition remain unknown. To clarify them, we investigated the 4DN pattern, mechanism and outcome in somatic cell reprogramming. We observed TAD shaking plays more important role in activating pluripotency genes in a super-enhancers (SEs)-dependent manner than compartment switch. OCT4 physically interacts and co-enriches with structuring factors to form OCT4 loops. The dynamics of OCT4 loops induce TAD shaking by regulating the chromatin binding of structuring factors at TAD boundaries. Novel Reprogramming Regulators (NRRs) were precisely identified by integrating the TAD Shaking-based Multiomics Analysis (TADSMAN), and knockdown most of the NRRs reduces reprogramming efficiency. Artificial construction of chromatin loop between SE and Dppa5a, one representative NRR, in MEF influenced TAD structure and enhanced reprogramming efficiency, supporting the reliability of the TADSMAN model.