FoxP3 ChipSeq
ChipSeq of FoxP3 bound regions and mRNAseq data of human Treg and CD4+ Th cells
The study contains two datasets. One represents a ChipSeq experiment for FoxP3 bound genes in human Treg and CD4+ Th cells after activation. Each cell type was sequenced in duplicates (two healthy donors). The second dataset contains mRNAseq data from Treg and CD4+ Th cells in resting and activated states.
Regulatory T-cells (Treg) play an essential role in the negative regulation of immune answers by developing an attenuated cytokine response that allows suppressing proliferation and effector function of T-cells (CD4+ Th). The transcription factor FoxP3 is responsible for the regulation of many genes involved in the Treg gene signature. Its ablation leads to severe immune deficiencies in human and mice. Recent developments in sequencing technologies have revolutionized the possibilities to gain insights into transcription factor binding by ChiP-Seq and into transcriptome analysis by mRNA-Seq. We combine FoxP3 ChiP-Seq and mRNA-Seq in order to understand the transcriptional differences between primary human CD4+ T helper and regulatory T-cells, as well as to study the role of FoxP3 in generating those differences. We show, that mRNA-Seq allows analyzing the transcriptomal landscape of T-cells including the expression of specific splice variants at much greater depth than previous approaches, whereas 50% of transcriptional regulation events have not been described before by using diverse array technologies.