Mastitis Metagenome
Milk microbiome signatures of subclinical mastitis-affected cattle
The goals of the present study was to undertake a comparative evaluation of microbial community and the metabolic profiling among the milk samples from three breeds of cattle harbouring subclinical mastitis. Studying the DNA and metabolic profiling of microbial communities could help to establish more effective prevention and treatment against potential microbial agents.   Subclinical mastitis-affected milk samples of Kankrej and Gir cattle (Bos indicus) were collected from Livestock Research Station (LRS) and samples of crossbred cattle (Bos taurus X Bos indicus) were collected from Livestock Production and Management (LPM) cattle farms in Anand town of Gujarat State, India. Quarter milk samples were collected aseptically in sterile wide mouth glass stopper bottles on three consecutive days. The udder was washed thoroughly with potassium permanganate solution (1:1000) and the teats were wiped with 70% ethyl alcohol prior to sampling.