GSE163188
SUM159 cells treated with H3B-8800 or DMSO, J2 enrichment for dsRNA
The objective is to identify splicing and expression changes that occur after spliceosome inhibition in triple-negative breast cancer, specifically after the RNA has been enriched for double-stranded structure using the J2 antibody. Overall design: SUM159 cells treated with H3B-8800 (spliceosome inhibitor) or vehicle (DMSO) for 12 hours in biological duplicate. Then RNA was enriched for double-stranded structure using the J2 antibody prior to library prep.