Mutational_burden_in_mouse_oesophagus__p53-sc-5199
Mutational_burden_in_mouse_oesophagus__p53
We wish to determine how the mutational burden in mouse oesophagus changes in transgenic mice induced to carry mutations known to cover significant areas of human oesophagus.  Oesophaguses from mice carrying either mutations in P53, Notch1 or with altered immune systems  will be collected and DNA extracted from 2mm2 biopsies.  These will then be sequenced at high depth to allow an assesssment of the mutational burden.  In some cases the mice will be further treated with DEN a carcinogen present in cigarette smoke.
We wish to determine how the mutational burden in mouse oesophagus changes in transgenic mice induced to carry mutations known to cover significant areas of human oesophagus.  In some cases the mice will be further treated with DEN a carcinogen present in cigarette smoke.  Ultilising targetted high-depth sequencing we will attempt to understand how the mutational landscape of the oesophagus changes with different background mutations.