GSE138248
mRNA sequencing of highly and lowly metastatic human colorectal cancer PDXs
We performed in-vivo selection of human patient derived colorectal cancer xenografts. 4 independent highly-liver metastatic sub-lines (lvm PDXs) were generated. Those lvm PDXs were harvested with the corresponding parental PDX tumors from mice and then we performed MACS mice cell depletion followed by FACS sorting to obtain human EpCAM positive and mice MHC negatitve populations. total RNA was extracted from those ex-vivo tumors and high-throuput sequencing was performed Overall design: Differential gene expression study between 4 independent highly-liver metastatic PDXs and their corresponding parental PDXs