Multiplex_Plasmodium_transgenesis__Sequence_based__quantitative_detection_of_pooled_P__berghei_transfectants-sc-2012-01-18T12:57:41Z-2086
Multiplex_Plasmodium_transgenesis__Sequence_based__quantitative_detection_of_pooled_P__berghei_transfectants
Data obtained from the sequencing of pools of barcoded P. berghei transgenics is predicted to allow for qualitative and quantitative measurements of individual mutant progeny generated during multiplex transfections. This type of analysis is expected to take P. berghei reverse genetics beyond that of the single-gene level. It aims to explore genetic interactions by measuring the effect on growth rates caused by simultaneous disruption of different genes in diverse genetic backgrounds, as well as potentially becoming a tool to identify essential genes to be prioritised as e.g. potential drug targets, or conversely to be excluded from future gene disruption studies. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
This project comprises the sequencing (Illumina MiSeq) of 100 bp PCR products amplified from pools of  P. berghei transfectants, derived from multiplex transfections performed with gene targeting vectors carrying unique, gene-specific 11 bp molecular barcodes.