GSE210700
Metabolomic and transcriptomic profiling of adult mice and larval zebrafish leptin mutants reveal a common pattern of changes in metabolites and signaling pathways [Dataset 2]
Leptin plays a critical role in the regulation of metabolic homeostasis. However, the molecular mechanism and cross talks between leptin and metabolic pathways leading to metabolic homeostasis across different species is not clear. This study aims to explore the effects of leptin in mice and zebrafish larvae by integration of metabolomics and transcriptomics. We performed gene expression profiling analysis using data obtained from RNA-seq. . Deep sequencing showed that many genes involved in proteolysis and arachidonic acid metabolism were dysregulated in ob/ob mice heads and lepb mutant zebrafish larvae compared to their wild type counterparts, respectively. Overall design: Male ob/ob mice and lean C57BL/6 wild type mice were obtained from Charles River Laboratories at 6 weeks of age (n=8 per group) and maintained for 8 weeks under specific pathogen-free conditions in the animal facility of the Leiden University Medical Center (LUMC). Heads were used to exctract RNA for RNAseq.