GSE119800
RNA binding of CBFB and hnRNPK
RNA immunoprecipitation assay followed by next generation sequencing (RIPseq) to identify RNAs bound by CBFB and hnRNPK in breast cells. Overall design: One repeat for RIPseq of CBFB and two repeats for hnRNPK. A stable cell line derived from MCF10A cells (ATCC, Cat: CRL-10317) expressing FLAG tagged CBFB was used for CBFB RIP using the FLAG antibody (Sigma, Cat: A2220). Parental MCF10A cells were used for hnRNPK RIP using hnRNPK antibody (Bethyl Laboratory, Cat: A300-674A).