GSE124578
RNA-seq on Fixed and Permeabilized Plasma cells
Here we developed a protocol to extract total RNA from fixed and permeabilized cells that were intracellularly stained and FACS isolated.  We applied these techniques to derive the transcriptome of influenza- and BCR isotype-specific antibody secreting cells or plasma cells.  These data reveal that ASC isotypes have more distinct transcriptional signatures than previously appreciated. Overall design: RNAseq on fixed, permeabilized, and sorted antibody secreting cells