ena-STUDY-SIB-24-06-2021-15:53:48:971-1153
Loss of TNIP1 leads to changes in expression of inflammatory genes
HeLa TNIP1 KO cells were generated by CRISPR-Cas9. Four replicates of each Hela wildtype (WT) and TNIP1 KO cells (clone1 and clone2) were cultured in 10 cm dishes. The total RNA was extracted using RNeasy Mini Kit (Qiagen) according to manufacturer`s instructions. The quality of RNA samples (RINe) was analyzed by RNA screen tape (Agilent). Complementary DNA (cDNA) libraries were barcoded using Illumina primers and sequenced on one lane of an Illumina Novaseq 6000 instrument with 2X50 bp paired-end sequencing cycles (NGS Sequencing Platform, Bern; 12 samples in total).
HeLa TNIP1 KO cells were generated by CRISPR-Cas9. Four replicates of each Hela wildtype (WT) and TNIP1 KO cells (clone1 and clone2) were cultured in 10 cm dishes. The total RNA was extracted using RNeasy Mini Kit (Qiagen) according to manufacturer`s instructions. The quality of RNA samples (RINe) was analyzed by RNA screen tape (Agilent). Complementary DNA (cDNA) libraries were barcoded using Illumina primers and sequenced on one lane of an Illumina Novaseq 6000 instrument with 2X50 bp paired-end sequencing cycles (NGS Sequencing Platform, Bern; 12 samples in total).