ena-STUDY-SLU-27-09-2016-13:12:20:975-508
Sequencing and annotation of equine MHC class II region
In this study we have sequenced, assembled and annotated equine MHC class II region. Eight bacterial artificial chromosome clones (CHORI-241 library, Thoroughbred horse Bravo) spanning the minimum tilling-path of  the equine MHC class II region and two long-range PCR products were sequenced with long-read PacBio technology. Obtained sequences were assembled in a single Bravo MHC class II sequence and 34 genes we located on this sequence.
In this study we have sequenced, assembled and annotated equine MHC class II region. Eight bacterial artificial chromosome clones (CHORI-241 library, Thoroughbred horse Bravo) spanning the minimum tilling-path of  the equine MHC class II region and two long-range PCR products were sequenced with long-read PacBio technology. Obtained sequences were assembled in a single Bravo MHC class II sequence and 34 genes we located on this sequence.