GSE99627
The functional enhancer repertoire of human embryonic stem cells [ChIP-seq]
We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the ChIP-seq and BAC component. Overall design: ChIP-seq for OCT4 (POU5F1), NANOG, H3K27ac, H3K4me1 + Input control in naive and primed hESCs = 14 datasets total + 1 BAC library