GSE196615
Transcriptomic changes in Sin3A deficient  CD4 T cells
Highly purified CD4+ T cells from Rosa26-CreERT2 +/- Sin3AF/F micewere then cultured in interleukin 7 (IL-7) in the absence or the presence of 4-hydroxytamoxifen (TAM) for 6 days  to allow Cre expression in the absence of cell division or differentiation . Next, we compared the behavior of Sin3A sufficient and deficient T cells in Th17 skewing conditions. RNA was then isolated and sequenced for the analysis of differential expression of mRNA transcripts Overall design: Sin3A deficient and Sin3A suficient CD4 T cells were cultured  for 3  days in Th17 polarizing conditions. mRNA was then purified and analyzed by RNA-sec