GSE211701
Developmental trajectories of thalamic nuclei revealed by single-cell transcriptome profiling and Shh perturbation
The thalamus is the principal information hub of the vertebrate brain, with essential roles in sensory and motor information processing, attention, and memory. The complex array of thalamic nuclei develops from a restricted pool of neural progenitors. We apply longitudinal single-cell RNA-sequencing and regional abrogation of Sonic hedgehog (Shh) to map the developmental trajectories of thalamic progenitors, intermediate progenitors, and post-mitotic neurons as they coalesce into distinct thalamic nuclei. These data reveal that the complex architecture of the thalamus is established early during embryonic brain development through the coordinated action of four cell differentiation lineages derived from Shh-dependent and independent progenitors. We systematically characterize the gene expression programs that define these thalamic lineages across time and demonstrate how their disruption upon Shh depletion causes pronounced locomotor impairment resembling infantile Parkinson's disease. These results reveal key principles of thalamic development and provide mechanistic insights into neurodevelopmental disorders resulting from thalamic dysfunction. Overall design: The thalamic primordia were micro-dissected from three embryos per control and SBE1/5 genotype according to anatomical landmarks at four developmental stages (E12.5, E14.5, E16.5, and E18.5) coinciding with peak periods of thalamic proliferation, neurogenesis, and differentiation (n=24 embryos in total).