GSE105815
Nascent-seq data from DRB-treated/untreated human U2OS cell line
Confluent U2OS cells were released to serum-free medium, and continued to grow for 36 h (Per2-dLuc peak) or 48 h (Per2-dLuc trough), then added DRB (10uM) treatment for 0.5, 1.5, 3.5 h, then added BrU (250uM) for 0.5 h, harvested total RNA, captured with anti-BrU antibody, and conduced hi-seq. Overall design: To determine the global effect of low-dose DRB on gene transcription in human cells