GSE119869
RNA-seq expression profiling of ductal macrophages and antigen presenting cells from virgin mouse mammary glands
Macrophages are diverse immune cells that reside in all tissues to regulate development, function and homeostasis.  In the mammary gland, a highly dynamic organ that undergoes dramatic remodeling throughout life, macrophages have been implicated in development and breast tumorigenesis. Ductal macrophages, as opposed to their stromal counterparts, were found to promote ductal morphogenesis during puberty and efficiently phagocytose alveolar cells post-lactation. Within mammary tumors, only ductal macrophages expanded to form a network throughout the tumor, where they also contact intra-tumoral T cells. Furthermore, ductal macrophages accelerated the growth of tumor organoids. Thus, pro-remodeling mammary ductal macrophages represent the normal tissue counterpart of tumor macrophages in an organ that is highly susceptible to oncogenesis. To gain insight into ductal macrophage function, we sorted all antigen presenting cell populations from mouse mammary glands and undertook RNA-seq expression profiling. This showed that ductal macrophages have a distinct expression profile from those of stromal macrophages or dendritic cells from the mammary gland. Results from this data series are presented in Figure 2 of Dawson et al (2020). Overall design: Two biological replicates were prepared of each of ductal macrophages, stromal macrophages, CD11b-CD103+ dendritic cells, CD11b-CD103- dendritic cells and CD11b+ dendritic cells from the mammary glands of adult, female, virgin mice. Paired-end 80bp RNA-seq reads were generated on an Illumina NextSeq 500 sequencer.