154ae791-4266-462e-87bc-440970785dfa
Bioprospecting for novel Coleopteran luciferase in unidentified museum specimens
DNA was non-destructively extracted from five unidentified dry-preserved Lampyridae from the National Museum Cardiff. The DNA was prepared for Illumina sequencing using the NEXTFLEX? Rapid DNA-Seq Kit for Illumina? Platforms (PerkinElmer, MA, USA), with the omission of sample sonication due to the degraded nature of the DNA extracts. The constructed libraries were enriched for luciferase gene sequences utilizing a cross-species affinity enrichment method which used biotin probes constructed from the Photinus pyralis luciferase gene cDNA. The enriched libraries were subsequently sequenced as 150 bp paired-end reads on the Illumina NextSeq 500 Sequencer (Illumina, Inc., CA, USA) in the Cardiff University BIOSI Genomics Research Hub.
DNA was non-destructively extracted from five unidentified dry-preserved Lampyridae from the National Museum Cardiff. The DNA was prepared for Illumina sequencing using the NEXTFLEX? Rapid DNA-Seq Kit for Illumina? Platforms (PerkinElmer, MA, USA), with the omission of sample sonication due to the degraded nature of the DNA extracts. The constructed libraries were enriched for luciferase gene sequences utilizing a cross-species affinity enrichment method which used biotin probes constructed from the Photinus pyralis luciferase gene cDNA. The enriched libraries were subsequently sequenced as 150 bp paired-end reads on the Illumina NextSeq 500 Sequencer (Illumina, Inc., CA, USA) in the Cardiff University BIOSI Genomics Research Hub.