8633:52931ebc-8309-42c6-ab81-1f66bb8d12c3
Pelagic eukaryotic communities across Fram Strait
Water samples were collected in Fram Strait during the Polarstern cruise PS85 (June 6th 2014                 until July 3rd 2014) from eastern Greenland shelf to west coast of Spitsbergen. For assessing eukaryotic                 community composition 2 L of water were filtered using vacuum filtration (at 200 mbar) through                 successive membrane filters of 10 µm , 3 µm and 0.4 µm. The extracted genomic eukaryotic DNA was pulled                 together from the different fractions and the hypervariable V4 (528iF - 964iR) region of the 18S rRNA                 was amplified and sequenced using Illumina MiSeq platform in a 2 × 300 bp paired-end run.