GSE95706
mRNA expression data from ESCs derived by polar body transfer reconstructed embryos (PBTESCs)
Here we report the derivation of human PBTESCs from polar body transfer resconstructed embryos. We used RNA-seq to compare the gene expression levels among human parthenogenetic haploid ESCs (hPGES)?normal human ESCs (H9) and human forskin fibroblasts and identified that these cells express conventional ESCs pluripotent markers and most maternally imprinted genes were down-regulated. Overall design: We collected fresh ESCs samples of 3 cell lines from PB1 transfer and 3 cell lines from PB2 transfer each at around 10^6 cells in similar passage. All the sample was lysised by 1 mL Trizol directly and stored at -80?. DNA libraries building and sequencing was performed at the same time to reduce systematic error. Gene expression profiles of all the cell lines were analysed on illumina Hiseq 2500.