PRJNA472613
Enhanced bacterial immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand DNA breaks
Fastq files associated with Figure 1 from the manuscript "Enhanced bacterial immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand DNA breaks". The fastq files are raw sequence reads generated from NGS on 2 pooled samples (each for a biological replicate). Each pooled sample contains 40 distinct PCR amplicons generated after genome editing. Editing frequencies can be obtained from the pooled samples through demultiplexing, using the reference information of sgRNA sequences and primer sequences in Supplmentary Information.