GSE162437
Gene expression profile of EphB6-depleted D2.0R-EGFP cells in vivo
We analysed gene expression profiles of D2.OR-EGFP-shControl and D2.OR-EGFP-shEpB6 cells isolated from lungs Overall design: D2.0R-EGFP-shControl (1x106 cells/mouse) or a mix of D2.0R-EGFP-shEphB6 cells (shEphB6#31, #34, #35) were injected in the tail vein of nude athymic BALB/c mice. After two weeks, lungs were harvested and digested into a single-cell suspension as described above. CD45-/EGFP+ cells were sorted directly into lysis buffer and total RNA was extracted with RNeasy Plus Micro Kit (Qiagen) following the manufacturer's instructions.