PRJNA674830
Genome resequencing of Cas9n generated mutants in Clostridioides difficile 630
We developed a CRISPR-Cas9 nickase genetic editing system for Clostridioides difficile in order to interrogate gene function of the accessory gene locus-1 (agr1). We generated gene deletion strains of agrB1, agrD1, and agrB1D1 in Clostridioides difficile 630 and re sequenced the genomes of each to confirm no off targets were created. We also sequenced our parental 630 strain for comparison.