GSE210669
Impact of aging on intestinal epithelial cells and reversible aspects by metformin or rapamycin treatment
Aging is a risk factor that initiates pathogenesis in the intestinal mucosa. We used single cell RNA sequencing to dissect the aging process and how effectively geroprotectors delays the process. Overall design: Intestinal epithelial cells from CB6F1 male mice were isolated by FACS (Epcam+, CD45 neg) at either 5-month-old (Young), or 24-month-old (Old). For treatment group, metformin or rapamycin treatment for old mice began at 21-month-old and sacrificed at 24-month-old.