E-MTAB-5941
Transcriptome profiling of short-term response to chilling stress in resistant and susceptible rice seedlings
The aim of this study is to profile the short-term transcriptional response of rice to chilling at different timings, and to identify genes that are putatively responsible for stress-tolerance. Thaibonnet and Volano were selected as susceptible and resistant rice cultivars, respectively. According to bibliography and to our quantitative PCR experiment results, we inferred that 2 and 10 hours after the beginning of stress are the time-points in which short-term “early” and “late” transcriptional response to chilling is more evident.  RNA from Thaibonnet and Volano plants after 0 (not stressed), 2 and 10 hours at 10°C were extracted and sequenced. Differential short-term responses to low temperatures at two different timings (to which we refer as “early” and “late”) between those two cultivars were analysed by RNA-seq.  Our study may contribute to elucidate the molecular mechanisms involved in rice response to chilling and to identify novel genes putatively involved in low temperature tolerance.
The aim of this study is to profile the short-term transcriptional response of rice to chilling at different timings, and to identify genes that are putatively responsible for stress-tolerance. Thaibonnet and Volano were selected as susceptible and resistant rice cultivars, respectively. According to bibliography and to our quantitative PCR experiment results, we inferred that 2 and 10 hours after the beginning of stress are the time-points in which short-term “early” and “late” transcriptional response to chilling is more evident.  RNA from Thaibonnet and Volano plants after 0 (not stressed), 2 and 10 hours at 10°C were extracted and sequenced. Differential short-term responses to low temperatures at two different timings (to which we refer as “early” and “late”) between those two cultivars were analysed by RNA-seq.  Our study may contribute to elucidate the molecular mechanisms involved in rice response to chilling and to identify novel genes putatively involved in low temperature tolerance.