GSE129402
Targeted deletion of Tcf7l2 in adipocytes promotes adipocyte hypertrophy and impaired gluose metabolism [HFD]
We generated a Tcf7l2 F/F mouse and harvested preadipocytes from these mice, immortalized them, and then transduced them with a retroviral vector containing CreERT2 and , differentiated them, performed ChIP-seq, and RNA-seq. We also generated an adipose specific knockout mouse where we crossed our Tcf7 F/Fmouse with an Adiponectin-Cre mice and performed RNA-seq on the inguinal white adipose tissue after 16 weeks of high fat diet, 65% Conclusions: Adipsose selective deletion of Tcf7l2 led to hypertrophic inguinal white adipose tissue and impaired glucose metabolsim with transcripts involved in lipid and glucose metabolsim being altered. Overall design: RNA-seq of WT or Tcf7l2 KO inguinal white adipose tissue from mice fed high fat diet for 16 weeks.