GSE192420
High throughput gene expression data from MDA-MB-231 breast cancer cell lines with DAXX Knockdown, DAXX WT overexpression (OE), and DAXX SUMO interacting motif mutant (DSM) overexpression (DSM OW)
DAXX, originally discovered as a context-dependent regulator of cell death or survival. Emerging evidence suggests that DAXX has an oncogenic role in diverse cancer types. However, the molecular mechanisms underlying DAXX's oncogenic function remain to be defined. We used high throughput Illumina sequencing to analyze genes that were differentially expressed upon DAXX knockdown or DAXX OE or DSM OE. Overall design: Stable expression of cDNA was established through lentiviral transduction of MDA-MB-231 cell line for WT OE or DSM OE and puromycin (2 µg/mL) selection. Scrambled control shRNA-transfected MDA-MB-231 cells were applying as control. Control MDA-MB-231 cells, Stable DAXX knockdown, DAXX OE and DSM OE cell lines were seeded with 2 x 10^5 cells at 10 cm culture plate. Duplicate cell cultures were conducted.