PRJNA451248
Oryza sativa cultivar:Nipponbare Transcriptome or Gene expression
Seeds of Oryza sativa (Nipponbare) were surface-sterilized with 20% bleach and 0.05% Tween-20 before treatments. Seeds (~30-50 individuals) were placed on petri dishes with wet filter paper and then were transferred to air control or hypoxia conditions under complete darkness. The hypoxia treatment was carried out using the Whitley H35 Hypoxystation (Don Whitley Scientific Limited, UK) with 3% O2 level at 28°C. Seed samples were harvested at 6 h after treatments and used for further transcriptomic and proteomic analysis.