PRJNA680435
Saccharomyces cerevisiae BY4741 Raw sequence reads
we developed a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produce carotene. These files are the sequences analysis of gRNA libraries in evolutionary strains.