GSE121086
Comparison of DNA methyltransferase-1 (Dnmt1) deleted urogenital sinus epithelium compared to wildtype urogenital sinus epithelium
Dnmt1 is an important regulator of tissue development and differentiation. To assess the effects of epithelium Dnmt1 deletion in the developing urogenital sinus (precursor of the urethra and prostate in males), we isolated urogenital sinus epithelial tissue from Dnmt1 deleted mouse embryos and wildtype mouse embryos. The transcriptomes were analyzed by RNA-seq Overall design: A Shh cre driver was used to achieve Dnmt1 deletion in the epithelium of the developing urogenital sinus. Epithelium of the urogenital sinus of embryonic day 15.5 mouse embryos were isolated by trypsin digestion. Tissues from Dnmt1 mutants (Dnmt1cKO) were compared to wildtype embryos (Control) in this experiment.