PRJNA504533
Promoter-intrinsic and local chromatin features determine gene repression  in lamina-associated domains
In this study we compared the response of 7 different promoters (ADAMTS1, ARHGEF9, BRINP1, MED30, PGK, TMEM106B, ZNF300) to various chromatin contexts. For each promoter 2 separate pools were created with 2 sequencing replicates for cDNA reads, genomic DNA reads used for normalization and iPCR reads used to identify sites of integrations. In another experiment, plasmids of ~100 barcodes for each promoter, were transiently transfected together without integration to measure relative activity of the promoters. This was done 2 times, 2 replicates each for promoters other than PGK and 1 time, 2 replicates for all 7 promoters mixed together.