GSE184581
The oxygen sensor Prolyl hydroxylase domain 2 regulates the in vivo suppressive capacity of regulatory T cells
The oxygen sensor PHD2 (prolyl hydroxylase domain 2) plays an important role in cell hypoxia adaptation by regulating the stability of HIF proteins (HIF1a and HIF2a) in numerous cell types including T lymphocytes. The role of oxygen sensor on immune cells, in particular on regulatory T cell (Treg) function, has not been fully elucidated. The purpose of our study was to evaluate the role of PHD2 in the regulation of Treg phenotype and function. We demonstrate herein that selective ablation of PHD2 expression in Treg (PHD2?Treg mice) leads to a spontaneous systemic inflammatory syndrome, as evidenced by weight loss, development of a rectal prolapse, splenomegaly, shortening of the colon and elevated expression of IFN-? in the mesenteric lymph nodes, intestine and spleen. PHD2 deficiency in Tregs led to an increased number of activated CD4 conventional T cells expressing an effector/Th1-like phenotype. Concomitantly, the expression of innate-type cytokines such as IL1-ß, IL-12p40, IL-12p35 and TNF-a was found to be elevated in peripheral (gut) tissues and spleen. PHD2?Treg mice also displayed an enhanced sensitivity to DSS-induced colitis and to toxoplasmosis, suggesting that PHD2-deficient Tregs do not efficiently control inflammatory response in vivo, in particular immune responses characterized by IFN-? production. Further analysis revealed that Treg dysregulation is largely prevented in PHD2-HIF2a (PHD2-HIF2a?Treg mice), but not in PHD2-HIF1a (PHD2-HIF1a?Treg mice) double KOs, suggesting an important and possibly selective role of the PHD2-HIF2a axis in the control of Treg function. Finally, the transcriptomic analysis of PHD2-deficient Tregs revealed an altered expression of several chemokine receptors including CXCR3, a finding corroborated by the altered in vivo localization of PHD2-deficient Tregs in splenic tissues. Collectively, these findings uncover an important role of the PHD2-HIF2a axis in regulatory T cell positioning and trafficking. Overall design: The goal of this study was to compare transcriptome of Treg cells from different mouse strain (Foxp3cre, PHD2?Treg, PHD2-HIF1a?Treg, PHD2-HIF2a?Treg and PHD2-HIF1a-HIF2a?Treg mice) to evaluate the role of PHD2-HIF2a axis on Treg phenotype. Splenic Treg cells were purified with anti-CD4 antibody and expression of YFP using Cell Sorter. The sample purity was assessed by Foxp3 staining (purity > 97%) .Total RNA was extracted using the TRIzol method and subsequently used to prepare RNA-Seq library by using TruSeq RNA sample prep kit (Illumina).