GSE129938
Transcriptome analysis of stomatal lineage ground cells (SLGCs)
Purpose: Identify SLGC cell fate by transcriptomes Methods: Cells expressing SLGC markers (BASLp:BRX-YFP, BASLp:myrBRX-YFP and myrBRXp:myrBRX-YFP) and stomatal commitment stage marker (MUTEp:MUTE-YFP) were collected by FACS. 9dpg plants were used for protoplasting and subjected to cell sorting. Around 20,000/sample were cellected and used for library preparation. Results: SLGCs are renriched in mitotic genes. In addition, top candidates, DEK and MYB16 proteins function in SLGC cell divisions. Conclusions: SLGCs are division competent cells and the coordination of cell cycle and transcriptional regulation though DEK is important for maintaining the transition (SLGC) state during stomatal development. Overall design: SLGC mRNA profiles of 9dpg seedlings were generated by deep sequencing, in duplicates, using Illumina HiSeq.