PRJNA514379
16S rRNA sequencing of IgG1-bound and unbound commensal bacteria (IgG1-seq)
Identification of mouse commensal bacteria that are recognized by serum IgG1. Mouse fecal commensal microbiota was stained with serum and secondary antibodies specific for mouse IgG1. IgG1-bound and unbound commensals were sorted by FACS and 16S rRNA sequencing was performed on the resulting fractions.