PRJNA497277
RNAseq over the course of T. gondii infection
Samples from uninfected or T. gondii infected 60 mm dishes of HFFs paired to infected metabolomics dishes were resuspended in TRIzol. RNA was extracted per the manufacturer's protocol, assayed for quality with an Agilent Bioanalyzer and Nano-6000 RNA chip, and synthesized into libraries with a TruSeq RNA Library Preparation Kit v2. Sequencing was performed through the UW Biotechnology Sequencing Center, using Illumina HiSeq2000.