ena-STUDY-EMBL-25-04-2018-18:56:19:387-11
EJC  ipaRt:  Transcriptome-wide  definition  of  stable  EJC  RNA  interactions  in  cytoplasm  of  adult  flies
The  eukaryotic  exon  junction  complex  (EJC)  is  essential  in  post-transcriptional  control  of  gene  expression.  EJC  is  deposited  at  mRNAs  during  nuclear  splicing  mainly  at  sites  located  -20  to  -24  nucleotides  upstream  of  de  novo  formed  exon-exon  junctions  which  have  been  defined  as  canonical  EJC  binding  sites.  We  present  here  a  novel  approach  for  clean  isolation  of  protein-complexes  associated  to  their  RNA  targets  (ipaRt),  a  prerequisite  to  investigate  cytoplasmic  EJC-mRNA  holo-complexes  in  adult  flies.
The  eukaryotic  exon  junction  complex  (EJC)  is  essential  in  post-transcriptional  control  of  gene  expression.  EJC  is  deposited  at  mRNAs  during  nuclear  splicing  mainly  at  sites  located  -20  to  -24  nucleotides  upstream  of  de  novo  formed  exon-exon  junctions  which  have  been  defined  as  canonical  EJC  binding  sites.  We  present  here  a  novel  approach  for  clean  isolation  of  protein-complexes  associated  to  their  RNA  targets  (ipaRt),  a  prerequisite  to  investigate  cytoplasmic  EJC-mRNA  holo-complexes  in  adult  flies.