GSE174435
Identifying METTL5-bound RNA by crosslinking-assisted immunoprecipitation
To identify the substrates of METTL5, we used crosslinking-assisted immunoprecipitation of overexpressed, FLAG-METTL5 followed by high throughput sequencing. Overall design: Comparing RNA isolated from input samples to RNA isolated from a FLAG immunoprecipitation of FLAG-METTL5 overexpressed in HeLa cells.