GSE133077
Enforcement of stem-cell dormancy by NPMc+ is a critical determinant of unrestricted self-renewal during myeloid leukemogenesis
Purpose: Analysis of downstream pathways activated by NPMc+ and Flt3-ITD oncogenes in preleukemic hematopoietic stem cells (HSCs). Methods: RNA extracted and processed from MxCRE, Flt3-ITD and NPMc+/Flt3-ITD FACS sorted HSCs Results: These data demonstrate that NPMc+ expression in Flt3-ITD HSCs promote a transcriptional program that supports both self-renewal and quiescence. Overall design: 3 LT-HSC samples have been sequenced (Mx, Flt3-ITD, NPMc+/Flt3-ITD). Biological replicates is provided. Mx samples should be considered the reference control.