GSE159143
Overexpression of NF110b and NF110b?DZF in Monocyte-Derived Dendritic Cells
We have found through previous experiments that ILF3 acts as a negative regulator of myeloid dendritic cell (DC) maturation. Mutagenesis experiments targeting various domains of both major isoforms of ILF3 (NF90b and NF110b) revealed that domain-associated with zinc finger (DZF) of NF110b was required for suppression of DC maturation. To determine which innate immune pathways may underlie the mechanism behind ILF3's suppression of DC maturation, we used lentiviral vectors expressing control (LKO), wildtpe NF110b, or  an NF110b DZF-deletion mutant in 4 individual donors. On day 5 post-transdiction, RNA was isolated and sent for total RNA sequencing. Differential gene expression between the wildtype and DZF mutant, relative to LKO controls, revealed enrichment for Cholesterol Homeostatis and Oxidative Phosphorylation pathways implicating involvement of these pathways in supression of DC maturaturation. Overall design: Examination of differentially expressed genes by wildetype NF110b and NF110b DZF deletion mutant (NF110b?DZF)