db906173-9790-4284-abca-dbbbac01ddbe
Genomic Explorations into an active Rift System
Sediment and rock samples from the unique, tectonically active Eger Rift (CZ) subsurface were recovered as part pf a 2019 scientific drilling campaign, with the goals to investigate the effect of mantle derived CO2 degassing and seismic activity on terrestrial subsurface ecosystems.  Analysis of 26 samples, covering depths between 17 m and 230 m revealed overall low biomass, but also demonstrated microbial life to persist in the terrestrial subsurface below 200m. Genomic explorations provided novel insights into a fascinating, potentially CO2 adapted, microbial community, dominated by rhizome and soil associated Proteobacteria and highlighted by an unexcepted archaeal diversity. The identification of acidophilic and methanogenic taxa suggests CO2 and potentially the periodically, through seismic activity released H2 to be important driving forces in this unique ecosystem. Reconstruction and annotation of several MAGs, from both core material and enrichments, provided first insights into microbial processes driven by CO2.
Sediment and rock samples from the unique, tectonically active Eger Rift (CZ) subsurface were recovered as part pf a 2019 scientific drilling campaign, with the goals to investigate the effect of mantle derived CO2 degassing and seismic activity on terrestrial subsurface ecosystems.  Analysis of 26 samples, covering depths between 17 m and 230 m revealed overall low biomass, but also demonstrated microbial life to persist in the terrestrial subsurface below 200m. Genomic explorations provided novel insights into a fascinating, potentially CO2 adapted, microbial community, dominated by rhizome and soil associated Proteobacteria and highlighted by an unexcepted archaeal diversity. The identification of acidophilic and methanogenic taxa suggests CO2 and potentially the periodically, through seismic activity released H2 to be important driving forces in this unique ecosystem. Reconstruction and annotation of several MAGs, from both core material and enrichments, provided first insights into microbial processes driven by CO2.