E-MTAB-7105
RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with BC094916 against vector control
We found that a novel gene BC094916 mRNA significantly decreased in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of BC094916 overexpression on plasmablast/plasma cells. BC094916 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV122 or LV201 (Fugene Corp., Guangzhou, China) to generate BC094916 and BC094916-EGFP fusion protein, respectively. BC094916-expressing LV122 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 µg/ml). BC094916 overexpression suppressed SP 2/0 cell proliferation by inducing cell apoptosis. Importantly, BC094916 overexpression effectively suppressed tumor progression in the SP 2/0 xenograft mouse model. In addition, we found that BC094916 is a suppressive transcriptional factor. To verify its target genes, we determined mRNA profiles in BC094916-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.
We found that a novel gene BC094916 mRNA significantly decreased in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of BC094916 overexpression on plasmablast/plasma cells. BC094916 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV122 or LV201 (Fugene Corp., Guangzhou, China) to generate BC094916 and BC094916-EGFP fusion protein, respectively. BC094916-expressing LV122 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 µg/ml). BC094916 overexpression suppressed SP 2/0 cell proliferation by inducing cell apoptosis. Importantly, BC094916 overexpression effectively suppressed tumor progression in the SP 2/0 xenograft mouse model. In addition, we found that BC094916 is a suppressive transcriptional factor. To verify its target genes, we determined mRNA profiles in BC094916-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.