ena-STUDY-UNIVERSITY OF BRISTOL-08-07-2020-13:28:28:643-1430
Comparison of Blood-borne bacterial population from orally healthy subjects and those with severe periodontitis
The use of Next Generation Sequencing (NGS) techniques has generated a wide  variety of blood microbiome data.  Due to the large variation in bacterial DNA profiles between studies and the likely high concentrations of cell-free bacterial DNA in the blood, it is still not clear how such microbiome data relates to viable microbiota.  For these reasons much remains to be understood about the true nature of any possible healthy blood microbiota and of bacteraemic events associated with disease. The gut, reproductive tracts, skin and oral cavity are all likely sources of blood-borne bacteria. Oral bacteria, especially those associated with periodontal diseases, are also commonly associated with cardiovascular diseases such as infective endocarditis and also have been linked to rheumatoid arthritis and Alzheimer's disease. Periodontal treatment, dental probing and toothbrushing have been shown to cause transient bacteraemia and oral bacteria from the Firmicutes  (e.g.Streptococci) and Bacteroidetes (e.g. Porphyromonas) are found in cardiovascular lesions (CVD).  Many other studies find Proteobacteria DNA to be the dominant blood microbiome component, suggesting a gut origin. Most studies of this type use total DNA extracted from either whole blood or blood fractions, such as buffy coat. Here, using a method that purifies DNA from intact bacterial cells only, we examined blood donated by those with unstable, severe periodontitis and periodontally healthy controls and show that 52% of bacterial species in blood are classified as oral. Firmicutes, consisting largely of members of the Streptococcus mitis group and Staphylococcus epidermis, were predominant at 63.5%  of all bacterial sequences detected in periodontal health and, little changed at 66.7% in periodontitis. Compared to studies that use total DNA extracted from blood fractions Proteobacteria were found at relatively low levels at 13.3% in periodontitis and 17.6% in health. This study also reveals significant phylogenetic differences in blood bacterial population profiles between periodontally healthy and unstable severe periodontitis cohorts.
The use of Next Generation Sequencing (NGS) techniques has generated a wide  variety of blood microbiome data.  Due to the large variation in bacterial DNA profiles between studies and the likely high concentrations of cell-free bacterial DNA in the blood, it is still not clear how such microbiome data relates to viable microbiota.  For these reasons much remains to be understood about the true nature of any possible healthy blood microbiota and of bacteraemic events associated with disease. The gut, reproductive tracts, skin and oral cavity are all likely sources of blood-borne bacteria. Oral bacteria, especially those associated with periodontal diseases, are also commonly associated with cardiovascular diseases such as infective endocarditis and also have been linked to rheumatoid arthritis and Alzheimer's disease. Periodontal treatment, dental probing and toothbrushing have been shown to cause transient bacteraemia and oral bacteria from the Firmicutes  (e.g.Streptococci) and Bacteroidetes (e.g. Porphyromonas) are found in cardiovascular lesions (CVD).  Many other studies find Proteobacteria DNA to be the dominant blood microbiome component, suggesting a gut origin. Most studies of this type use total DNA extracted from either whole blood or blood fractions, such as buffy coat. Here, using a method that purifies DNA from intact bacterial cells only, we examined blood donated by those with unstable, severe periodontitis and periodontally healthy controls and show that 52% of bacterial species in blood are classified as oral. Firmicutes, consisting largely of members of the Streptococcus mitis group and Staphylococcus epidermis, were predominant at 63.5%  of all bacterial sequences detected in periodontal health and, little changed at 66.7% in periodontitis. Compared to studies that use total DNA extracted from blood fractions Proteobacteria were found at relatively low levels at 13.3% in periodontitis and 17.6% in health. This study also reveals significant phylogenetic differences in blood bacterial population profiles between periodontally healthy and unstable severe periodontitis cohorts.