GSE194274
Single cell RNA-seq data for mouse fallopian tube and ovary
Epithelial ovarian cancer (EOC) is the most lethal malignancy of the female reproductive system. In order to improve EOC patient outcomes, it is crucial to have a better understanding of how EOC develops from its cellular origin. EOCs can originate from either fallopian tube epithelial (FTE) cells or ovarian surface epithelial (OSE) cells, but with different courses of development. The basis for this difference is unclear. To address this, we performed single cell RNA-sequencing of mouse cells isolated from the distal portion of fallopian tubes (i.e., oviducts) and surface layer of ovaries. Analysis of this single cell dataset revealed distinct niche organizations for murine FTE cells and OSE cells. Overall design: Fallopian tube and ovarian tissues from two wild-type adult FVB female mice were isolated. Single cells were prepared from the distal half (i.e., the portion close to ovary, thus including the fimbrial region) of fallopian tubes and from the stripped surface layer of each ovary. The resulting single cells were subjected to barcoding using the inDrop platform. The resulting expression libraries were then subjected to high throughput sequencing.