PRJNA479546
Base editing for soluble expression PACE (SE-PACE) evolved APOBEC
Soluble expression phage-assisted continuous evolution (SE-PACE) to improve expression of poorly expressed proteins. Rat APOBEC1 (rA1) has been utilized for site-specific base-editing. Utilization of SE-PACE yielded rA1 variants with better expression which also corresponded to improved activity when placed in a base editing context.