Yeast_KO_Seq_Strategy_1___PILOT_FOR_STRATEGY_2_COMPARISON__Mutational_signatures_of_DNA_damage_and_repair_processes-sc-3401
Yeast_KO_Seq_Strategy_1___PILOT_FOR_STRATEGY_2_COMPARISON__Mutational_signatures_of_DNA_damage_and_repair_processes
Part of a pilot study to compare 2 sequencing strategies. This will inform sequencing strategy for further work on the mutational signatures project, which seeks to identify mutational signatures attributable to all viable S.cerevisiae gene knockouts (approx. 4000). In this study, 8 samples from each knockout will be grown from single propagation line with a ~10,000 cell bottleneck. These will then be sequenced 96 samples per lane (12 strains/8 colonies per strain).  Data will then be compared against the same knockout strains sequenced as per SScape Study 3318.
This work is part of the broader WTSA investigating mutational signatures of DNA damage and repair processes.  Mutational signatures attributable to all viable S.cerevisiae gene knockouts will be determined.