GSE183222
Transcriptional response of human enteroids/colonoids on transwells to infection
The goal of this study was to evaluate the transcriptional response of human enteroids/colonoids on transwells to infections (bacterial and rotavirus). Enteroids/colonoids lines C103, C109, D103, D109, I103, I109, J2 and J11 were plated on transwells coated with Matrigel, differentiated, and inoculated (rotavirus (Ito), bacteria or mock) for 6 or 24 hours. Subsequently, total RNA was isolated and paired-end sequencing was performed. Overall design: Enteroid/colonoid mRNA profiles from 48 samples (no technical replicates) were generated by paired-end sequencing.