GSE115381
Transcriptome analysis of wt1b:eGFP positive macrophages in the regenerating zebrafish heart
Little is known about zebrafish macrophage subtypes and their contribution to organ regeneration. Using the transgenic line wt1b:eGFP we identified  a subtype of macrophages in the regenerating heart  that is transcriptionally different from the rest of macrophages. Overall design: Hearts from Tg(wt1b:eGFP; mpeg1:mCherry) zebrafish were cryoinjured and at 4 dpi mCherry and GFP/mCherry cells were FAC sorted. RNA was extracted from 4 pools of GFP/mCherry and 4 pools of mCherry single positive cells and  RNAseq was performed.