GSE43109
The role of HIF-1a and HIF-2a in hypoxic and M2-polarized macrophages
Macrophages (M?) skewn towards a regulatory-like phenotype are known to promote tumor progression. They tend to accumulate in hypoxic tumor areas and activate hypoxia-inducible factors (HIF-1 and HIF-2). HIFs are known to alter the gene expression profile of M?. To define direct HIF-1 and HIF-2 target genes in hypoxic and IL-10 treated human M? we used chromatin immuno-precipitation-sequencing (ChIP-seq) and microarray analysis on a genome-wide scale. Overall design: ChIP-seq of HIF1a and HIF2a in human macrophages under hypoxic and normoxic conditions with and without Interleukin-10 treatment