GSE113009
Next Generation Sequencing (NGS) of expression profile from unstimulated  and LPS stimulated bone marrow derived macrophages from control and BRD4 conditional KO mice
Method:  mRNA profiles were generated from pair-end sequencing of duplicate samples using Illumina  Hiseq 2000. Results: Genes with an expression change of more than 2 fold were  considered to be differentially expresed Overall design: Macrophages are cells belongs to innate immune system, which  response to pathogen by the production of inflammatory proteins those that are effective in both combating pathogen and wound healing. Using microarray approach with BET inhibitors  it was shown that many of the inflammatory response genes were under control of BET proteins.  Purpose of this study was  to assess the effect of BRD4 KO in NGS derived transcriptome profiles of both stimulated and unstimulated macrophages.