PRJNA60769
Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Impact of Capillary Length on Sequence Quality
The impact of sequencing conditions on sequence quality was evaluated by sequencing a variety of samples, including HMP even Mock community and clinical samples. The following PCR parameters were tested in addition to the original protocol: lower cycle number (20x), and longer extension time (10 min.)