PRJNA504919
Mus musculus strain:C57BL/6 | isolate:S1 | breed:B6.129P2-Apoetm1Unc/J | cultivar:saline Raw sequence reads
Electronic cigarette or electronic nicotine delivery systems (ENDS) are becoming popular in the world as an alternative to conventional nicotine cigarettes, both in smokers and people who have never smoked. To study the cardiac effect of ENDS, we used ApoE knockout (ApoE-/-) mice, the most commonly used murine model to study the cardiovascular effects of conventional cigarettes. Mice were exposed to ENDS aerosol from bluCig PLUS Classic tobacco ENDS containing high (2.4% nicotine) and for controls, mice were exposed to saline aerosol for 12 weeks. After RNA isolation from left ventricle, RNA quality was assessed with an Agilent 2100 Bioanalyzer. RNA integrity number was >8.0 for all samples. Sequencing was performed on Illumina HiSeq 3000 for a single read 50 run. Data quality check was done on Illumina SAV. Demultiplexing was performed using Illumina Bcl2fastq2 v 2.17 program. Differentially expressed genes were identified using the edgeR program. Genes showing altered expression with p < 0.05 and more than 1.5 fold changes were considered differentially expressed. The analysis, with the ingenuity pathway analysis software, revealed that deregulation of inflammatory, circadian rhythm and leucocyte extravasation signaling. Therefore, RNA-seq data unveils the induction of an inflammatory phenotype by ENDS. This transcriptomic genes further supports a role for ENDS in the development of a reactive oxygen species-driven cardiac dysfunction that systolic function.