E-MTAB-11206
RNA-seq of four-week-old shoot tissues of wild type (Col-0) and dph1 mutants (in Col-0 background) growing in soil under normal growth conditions
This is comparative transcriptomics by using the wild type and dph1 mutants to see the change of dph1 mutants at transcript levels compared with wild type. Rosette tissues of four-week-old plants grown in soil were harvested for total RNA isolation by RNeasy Plant Mini Kit (Qiagen). Libraries were prepared and sequenced using the Illumina platform by Novogene (Hongkong, CN). For RNA-seq data analysis, raw reads were first filtered by CutAdapt 2.1 to remove adaptors and low quality reads. The filtered reads were mapped to Arabidopsis thaliana Col-0 reference genome (Tair 10). Read counts were determined by Qualimap2. Differentially expressed genes were identified using the R package DESeq2.
This is comparative transcriptomics by using the wild type and dph1 mutants to see the change of dph1 mutants at transcript levels compared with wild type. Rosette tissues of four-week-old plants grown in soil were harvested for total RNA isolation by RNeasy Plant Mini Kit (Qiagen). Libraries were prepared and sequenced using the Illumina platform by Novogene (Hongkong, CN). For RNA-seq data analysis, raw reads were first filtered by CutAdapt 2.1 to remove adaptors and low quality reads. The filtered reads were mapped to Arabidopsis thaliana Col-0 reference genome (Tair 10). Read counts were determined by Qualimap2. Differentially expressed genes were identified using the R package DESeq2.