B_1_B_cells___a_new_source_of_macrophages_in_homeostasis_and_during_inflammation-sc-2013-08-29T13:31:10Z-2753
B_1_B_cells___a_new_source_of_macrophages_in_homeostasis_and_during_inflammation
To discern the origin of YFP+ M?s we performed next-generation sequencing of genomic DNA locus encoding immunoglobulin heavy chain (IgH) genes, which is solely re-arranged in B cell lineage. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The ‘classical’ model of haematopoiesis postulates that haematopoietic stem cells make an early and irrevocable choice between lymphoid and myeloid cell fates. However, recent data suggest a close developmental relationship between myeloid and lymphoid lineages. Here we first demonstrate evidence of lineage switch from B-1 B lymphocyte to a macrophage (M?), or so-called transdifferentiation, occurring in mature cells in vivo. Specifically, using transgenic B6.mb1.iCre::Rosa26-YFP mice, in which YFP expression is restricted exclusively to B cell lineage, we discovered that a significant portion (3-12 %) of tissue-resident M?s in peritoneum, pleural cavity and intestine in steady state are positive for YFP.