PRJNA435943
Lateolabrax japonicus Transcriptome or Gene expression
Lateolabrax japonicus is one of the main marine aquatic fish species, and is popularly cultured in East Asia due to its highly commercial value. In this research, RNA-Seq analysis of the L. japonicus were carried out to identify reproduction and growth related genes at L. japonicus ovary of different tree ages by Illumina sequencing technology. In total, 334,388,688 high quality reads were obtained in four libraries, i.e., four-year-old vary (4th_Ovary), three-year-old vary (3rd_Ovary), two-year-old vary (2rd_Ovary) and one-year-old vary (1st_Ovary), and de novo assembled into 101,860 Unigenes with an average unigene length of 879 bp. A total of 30,142 unigenes (29.59%) were annotated in the public databases: Nr database (Nr), Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Cluster of Orthologous Groups of proteins (COG), based on sequence similarity. Comparative analysis revealed, 35,749, 43,657 and 36,819 differentially expressed genes (DEGs) were identified in three comparisons (4th_Ovary VS 3rd_Ovary, 4th_Ovary VS 2rd_Ovary and 4th_Ovary VS 1st_Ovary), in total 24,295 DEGs were different expressed in 4th_Ovary. Enrichment and pathway analysis of the DEGs was also carried out to excavate the candidate genes related to reproduction and growth. The present study enriches the genomic resources of L. japonicus and provides fundamental information for further studies.