GSE111732
Comparative Analysis Of miRNA Profile In Human Dendritic Cells Infected With Respiratory Syncytial Virus And Human Metapneumovirus
Background. Human Metapneumovirus (HMPV) and Respiratory Syncytial Virus (RSV), are responsible for respiratory diseases mostly in children. In spite of the resemblance between these two pneumoviruses, they elicit a different extent of immune response. miRNAs are small non coding RNAs that regulate gene expression and are involved in numerous cellular processes including the immune system.  Methodology. Human monocyte-derived dendritic cells (moDC) were differentiated from peripheral blood mononuclear cells and infected at an MOI of 3 for 24h. RNA was isolated to analyze the miRNAs transcription by high throughput sequencing using illumina technology.  Principal findings. Infection with HMPV up-regulated the expression of hsa-miR-4448, while RSV infection induced significant expression of hsa-miR-30a-5p, hsa-miR-182-5p, hsa-miR-1913, hsa37, miR-4448 and hsa-miR-4634.  Conclusions/Significance. In human monocyte derived dendritic cells (moDC), RSV and HMPV induced different profiles of miRNA expression. Understanding the changes of miRNA expression profiles by RSV and HMPV in immune cells will further our understanding of the differential immune response induced by these respiratory viruses Overall design: miRNA Profile of non-infected and RSV- or HMPV-infected monocyte derived-dendritic cells (moDC)