GSE106577
Nucleosome profiles for mammalian tissues [RNA-Seq]
Chromatin structure affects gene splicing and nucleosome as the basic packaged unit is associated with exon recognition. But little is known about the function of nucleosome occupancy in the process of exon origination. Here we performed MNase-seq to obtain genome-wide nucleosome profiles for several tissues of human, rhesus monkey, treeshrew, mouse and pig. At the first time we found conserved nucleosome profile for different tissues. By combining RNA-seq data of each species, we traced the nucleosome occupancy changes of human new exons. Surprisingly, we found nucleosomes were higher occupied before exon formation. And this pre-occupancy contributed to the formation of exon-intron GC difference and maintain splice site strength. Thus we proposed a preadaption model for the function of nucleosome occupancy in human exons origination. Overall design: Nucleosome profiles and transcriptome for multiple tissues of human, monkey, tree shrew, mouse and pig.