PRJNA60767
Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Impact of PCR on Chimera Formation
The impact of PCR conditions on chimera content was evaluated by dideoxy sequencing the HMP even Mock community, targeting the V3-V5 region. The following PCR parameters were tested in addition to the original protocol: lower cycle number (20x), and longer extension time (10 min.)