ena-STUDY-INRA (Nouvelle-Aquitaine-Bordeaux)-28-03-2019-14:26:22:168-157
Sequence based microsatellite genotyping development for Quercus faginea and Quercus canariensis.
Previously developed microsatellite markers in Quercus robur and Quercus petraea have been optimized for the development of a sequence based microsatellite genotyping approach in Quercus faginea and Quercus canariensis. A total of 60 loci were amplified in a multiplexed PCR and sequenced for 46 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 40 high quality loci were validated. This method has then been applied to genotype additional individuals.
Previously developed microsatellite markers in Quercus robur and Quercus petraea have been optimized for the development of a sequence based microsatellite genotyping approach in Quercus faginea and Quercus canariensis. A total of 60 loci were amplified in a multiplexed PCR and sequenced for 46 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 40 high quality loci were validated. This method has then been applied to genotype additional individuals.