P. cubensis 454 Sequence
454 genome sequencing of Pseudoperonospora cubensis reveals effector proteins with a putative QXLR translocation motif
Sequencing of Ps. cubensis genomic DNA isolated from sporangia was performed using the 454 GS-FLX Titanium Sequencer (Roche) at the Michigan State University Research Technology Support Facility. The procedure was described previously (Quinn et al. 2008). The reads were screened for contaminated cucumber DNA using the available sequence data from National Center for Biotechnology Information (NCBI) databases. After the identifiable cucumber sequences were removed, the remaining reads were assembled using the Roche gsAssembler (version 1.1.03).