GSE185217
Gene expression in MHCII restricted mature thymocytes from Thpok-RKF- or Thpok-RKF+ Thpokfl/flRunx3fl/dYFPCd4-Cre+ mixed Bone marrow chimera.
Thpok-RKF which harbors a mutation in the BTB domain and disrupts the interaction between NuRD complex could not repress CD8 and CD8 lineage genes expression in Thpokfl/flRunx3fl/dYFPCd4-Cre+ from B2m-/- host. However, Thpok-RKF upregulates CD4 expression in these mice. Overall design: Bone marrows from Thpokfl/flRunx3fl/dYFPCd4-Cre+ Thpok-RKF- or Thpok-RKF+ were mixed with CD45.1+CD45.2+ wild type bone marrow and transplanted into irradiated CD45.1+B2m-/- host mice. CD4+ SP, CD8+ SP, CD4+CD8+ cells from indicated mice were sorted. Gene expression was determined by RNA-seq.