ena-PROJECT-Genoscope CEA-22-08-2012-13:31:05:720-13
Tara-oceans samples barcoding and shotgun sequencing
This project  will rely on samples collected during the scientific expedition Tara-Oceans (2009-2012). By March 2012, the schooner Tara, equipped with innovative systems for sampling of 11 organismal size-ranges covering entire planktonic communities from viruses to animals, has collected standardized genetic (total DNA/RNA), morphological, and physico-chemical (contextual) samples from 153 sites across the world oceans, locations carefully selected with input from near-real-time remote sensing and in-situ hydrographic criteria. Overall, a total of ~50,000 biological samples and ~13,000 contextual measures from 3 depths will be analysed. The metagenomics component of the project consists of size fractionated plankton samples that are submitted to barcoding and shotgun sequencing, as well as isolated single-cell amplified protists and single-organisms isolated metazoans that are sequenced as reference genomes.
This project  will rely on samples collected during the scientific expedition Tara-Oceans (2009-2012). By March 2012, the schooner Tara, equipped with innovative systems for sampling of 11 organismal size-ranges covering entire planktonic communities from viruses to animals, has collected standardized genetic (total DNA/RNA), morphological, and physico-chemical (contextual) samples from 153 sites across the world oceans, locations carefully selected with input from near-real-time remote sensing and in-situ hydrographic criteria. Overall, a total of ~50,000 biological samples and ~13,000 contextual measures from 3 depths will be analysed. The metagenomics component of the project consists of size fractionated plankton samples that are submitted to barcoding and shotgun sequencing, as well as isolated single-cell amplified protists and single-organisms isolated metazoans that are sequenced as reference genomes.