GSE121795
Assessement of transcriptional consequences of CD27 signaling on Tregs during Th17 polarization
The goal of this study was to identify transcripts that were differentially expressed in untreated vs FcCD70 treated Tregs cultured in Th17 promoting conditions. Overall design: Tregs were sort purified from FOXP3-GFP reporter mice and cultured with anti-CD3/anti-CD28 coated Dynalbeads in Th17-polarizing conditions in the presence or absence of FcCD70. On day 6 we performed whole transcriptome RNAseq on purified cells.