GSE183823
Genome-wide chromatin binding of Hdac2 in murine PDAC cells (ChIP-Seq)
Hdac2 ChIP-seq were used to determine epigenetic differences between Hdac2 knockout versus Hdac2 wildtype pancreatic cancer cells. DOI: 10.1158/0008-5472.can-20-3209 Overall design: Genomic DNA was extracted and genome-wide chromatin binding of Hdac2 was determined in a Hdac2 proficient vs Hdac2 deficient setting. DNA of 2 replicates per setting were harvested and analyzed. Input DNA of all samples was pooled and used as background for MACS2 peak calling. Called peaks were then differentially analyzed using DiffBind (Proficient vs deficient).