GSE67148
Genome-wide maps of nucleosome positions in purified quiescent S. cerevisiae cells [MNase-Seq]
Nucleosome positions were determined for purified S. cerevisiae quiescent cells and compared to logarithmically growing cells. By analyzing nucleosome positions and MNase digestion patterns for two separate matched digestion amounts (~80% and ~50% mononucleosomes) we show that global nucleosome stabilization and repositioning occurs at transcription start sites. This repositioning indicates a repressive chromatin architecture that reflects global transcriptional shutoff in quiescent yeast. Overall design: Two matched MNase digests from two independent prototrophic W303 isolates during log growth (OD600=0.4-0.6) or from purified quiescent cells (pellet in Percoll gradient from 7-day stationay phase cultures) were subject to paired-end sequencing.