GSE184043
H3K36me3 and H3K27me3 occupancy profiling by high throughput sequencing from setd2 wt and ko germinal center B cells and  H3K27me3 occupancy profiling by high throughput sequencing from phf19 wt and ko germinal center B cells
Our study aims to understand the H3K36me3 and H3K27me3 genome-wide alterations by analysing CHIP-seq data between setd2 wt and ko germinal center B cells, characterize the function of H3K36me3 methyltransferase setd2 in H3K36me3 itself and H3K27me3, further  we aim to H3K27me3  from phf19 ,which is H3K27me3 regulator,  WT and KO group to investigate the mechanism of H3K36me3 dependent H3K27me3 modification introduced by setd2. Overall design: 4×10e6 germinal center B cells were sorted from SRBC immunized setd2 wt and ko  then flash frozen on dry ice for CHIP, and   germinal center B cells were sorted from SRBC immunized phf19 wt ko mice then flash frozen on dry ice for  cut&tag