PRJNA381328
CaMKII-delta isoform verification using SMRT sequencing
To systematically analyze cardiac CaMKII-delta isoforms in different species, we performed single molecular real-time sequencing (SMRT, Pacific Biosciences) of left ventricles from mouse, rhesus monkey, and human. Before SMRT sequencing, we amplified all of the CaMKII-delta isoforms with paired primers located on the first (exon 1) and last exons (exon 22).