ena-STUDY-Cambridge Sequencing Services (CSS)-28-03-2020-10:14:02:174-3
Magnetic bead baiting and MinION sequencing and assembly of complete chloroplast genomes from Malus species and apple cultivars
Based on tRNA sequences, capture primers were designed to isolate chloroplast genomes from total DNA. The chloroplasts were size fractionated, sequenced with MinION and assembled. Chloroplasts were annotated, aligned and employed for phylogenetic studies.
Based on tRNA sequences, capture primers were designed to isolate chloroplast genomes from total DNA. The chloroplasts were size fractionated, sequenced with MinION and assembled. Chloroplasts were annotated, aligned and employed for phylogenetic studies.