PRJNA837643
Breaking Barriers with Bread
Widespread usage of high throughput sequencing (HTS) in Life Sciences has produced a demand for undergraduate and graduate institutions to offer class exposing students to all aspects of HTS (sample acquisition, laboratory work, sequencing technologies, bioinformatics, and statistical analyses). Despite the increase in demand, many challenges exist for these types of classes. We advocate for the usage of the Sourdough starter microbiome for implementing meta amplicon sequencing. The relatively small community, dominated by a few taxa, enables potential contaminants to be easily identified, while between sample differences can be quickly assessed statistically. Finally, bioinformatic pipelines and statistical analyses can be carried out on students laptops or in a teaching computer lab. In two semesters adopting this system, 12 of 14 students were able to effectively capture the Sourdough starter microbiome, using the instructors paired sample as reference.