GSE195668
Lariat mapping of wild type and DBR1 KO cells after knockdown of AQR
Using wild type HEK293T cells and our DBR1 CRISPR knockout cell line (C22), we investigated the effect of AQR knockdown on cellular lariat levels. Samples were taken after transfection with either one of two AQR-targeting siRNAs or a non-targeting control siRNA. After sequencing the samples, lariat mapping of the reads revealed that AQR knockdown increased the lariat recovery rate by ~50% relative to the level observed in the negative control samples. Overall design: Lariat mapping of wild type and DBR1 KO cells treated with AQR siRNAs