PRJNA493297
Dynamics of chromatin accessibility during human forebrain development
Human forebrain development is characterized by a series of highly synchronized cellular events, which, if perturbed, can cause neurodevelopmental disease. Dynamic patterns of activity in non-coding regulatory DNA are thought to coordinate these events during development. To chart these activity patterns in the human forebrain, we used the assay for transposase-accessible chromatin and sequencing (ATAC-seq) to generate a map of accessible chromatin in isolated cell types from three-dimensional (3D) cultures. These cultures were derived from human induced pluripotent stem (hiPS) cells, resembled either the dorsal (cortical, pallial) or ventral (subpallial) domains of the human forebrain, and were maintained for up to 20 months in vitro.