PRJNA453764
Pinus taeda NCSU GBS Demultiplexed raw sequence reads
1,562 samples of tree phloem were collected from the Plant Seed Source Selection Study (PSSSS) located in Screven County, Georgia. The PSSSS consists of 140 pollen mix families planted in 20 test locations representing much of the loblolly pine range. Samples were digested with PstI and MspI restriction enzymes. Each of the libraries were sequenced for 100bp paired end reads over 4 flowcell lanes (~96 samples/lane) on an Illumina HiSeq2000 using V3 chemistry. A total of 16 flowcell lanes were sequenced by the Beijing Genomics Institute service center in Philadelphia. Plates were separated from the lanes by index and samples were split by barcode using fastx toolkit and a custom script.