GSE107814
RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes after Twist1 knockout in mouse bone marrow stromal cells
Purpose: The goals of this study are to determine possible  downstream targets of Twist1  in bone marrow stromal cells  with or without Twist1 knockout. Methods: gene expression profiling with or without Twist1 knockout in bone marrow stromal cells  were generated by deep sequencing, using Illumina Hiseq.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using  SYBR Green assays. Conclusions: Notch signaling were activated after Twist1 knockout. Overall design: mRNA profiles of stromal cells   with or without Twist1 knockout were generated by deep sequencing using Illumina Hiseq.