GSE173565
Single cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks.
Comparison of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin Epcam- cells and CD45-Epcam- stromal cells from lamina propria at the age of 11 wks. Two male mice per group was used in the experiment. Overall design: We analyzed two samples, aiming with both samples to 8,000-10,000 cells. EDTA treatment 20min +37°C. Liberase TL treatment 60min +37°C. FACS: DAPI-Ter119-Epcam-CD45- intestinal stromal cells