E-MTAB-8362
RNA-seq to assess the impact of circulation on hematopoietic stem cell differentiation.
Blood flow promotes hematopoiesis, Definitive hematopoietic stem cells can not be assessed in circulation negative Ncx-/- mice.  This study looks at the impact of lack of blood flow on the earliest blood cell precursors, the pro-HSCs.  E9.5 was chosen as this is the time point pro-HSCs are normally generated.  Cells from the hemogenic endothelium, the endothelium and pro-HSCs were sequenced.
Blood flow promotes hematopoiesis, Definitive hematopoietic stem cells can not be assessed in circulation negative Ncx-/- mice.  This study looks at the impact of lack of blood flow on the earliest blood cell precursors, the pro-HSCs.  E9.5 was chosen as this is the time point pro-HSCs are normally generated.  Cells from the hemogenic endothelium, the endothelium and pro-HSCs were sequenced.