GSE209671
Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion
Whether or not human Tregs are susceptible to exhaustion, and if so, if this would limit their therapeutic effect, was unknown. We studied how a tonic-signaling CAR, which induces Tconv exhaustion, affects human Tregs. We thus sorted naïve Tregs and naïve conventional CD4s and transduced them with a non-TS CAR (CD19) or a TS-CAR (HA). Overall design: 3 TCONV CD19 CAR samples, 4 TCONV HA CAR samples, 5 TREG CD19 CAR samples, 5 TREG HA CAR samples