GSE128263
Cadmium exposure induces SNAIL1 expression via downregulation of miR-30e in human bronchial epithelial cells
Purpose: To understand the molecular mechanisms underlying Cd exposure-induced diseases. Methods: Immortalized human bronchial epithelial cells (BEAS-2B) were cultured in Dulbecco's  Modified Eagle's Medium (DMEM, Cellgro) supplemented with 1% Penicillin Streptomycin and  10% Fetal Bovine Serum (FBS, Atlanta Biologicals) at 37 degree C and 5 % CO2. For Cd exposure,  0, 2.5, 5 and 10 µM of CdCl2 was added to the media and the cells were cultured for 72h. Results: This study shows that cadmium exposure induces SNAIL1 expression via miR-30e  downregulation and the cells undergo epithelial-mesenchymal transition. Overall design: RNA Seq gene expression comparision done in replicates