Chromatin_factors_in_stem_cells-sc-3238
Chromatin_factors_in_stem_cells
Chip-Exo of various factors (BRD4, DNMT1, H3K27ac and Tet3) in ES cells under either serum conditions or 2i conditions. TET3 chip-exo was also performed from mouse E13.5 brain Factors were identified as important epigenetic regulators in serum to 2i transition where cells undergo global demethylation and exhibit a more naïve stem cell state..
The primary objective of this study is to determine the validity of targets and antibodies potentially involved in regulation of stem cell epigenetics. Successful ChIP analysis will then be used to compare differences between 2i treated and serum treated mouse ES cells. Global quantitative and qualitative ChIP changes will then be analysed to understand their regulatory roles.