GSE107212
AMPKa1 and a2 knockout and usage of alternative polyadenylation sites
Role of alternative polyadenylation in muscle development remains largely undetermined.  Our WTTS-seq approach to capture 3''-end of RNAs clearly revealed alternaitve polyadenylation events responsible for reduced muscle mass in AMPK a1, but increased muscle mass in AMPK a2 knockout mice. Overall design: Total RNA samples obtained from muscle tissue of knockout (KO) mice and wild-type (WT) mice were used to construct WTTS-seq libraries. Eight WTTS-seq libraries including two AMPKa1 KO mice, two AMPK a2 KO mice, two AMPKa1 WT mice and two AMPK a2 WT mice were involved in this submission.