GSE184239
Next Generation Sequencing of Ad-Ctrl sh and Ad-Tox4 sh treated mouse livers
To interrogate the genome-wide target genes of TOX4, we performed RNA-seq from livers of 16-hr fasted animals (10-wk-old C57BL/6J male mice) following TOX4 KD. The RNA-sequencing was performed by Columbia Genome Center. Poly-A pull-down was used to enrich mRNA from total liver RNA and library construction with Illumina TruSeq. Libraries were sequenced using Illumina NovaSeq 6000. Kallisto pipeline was used to quantify transcript abundance (Bray et al., 2016). Overall design: Liver mRNA profiles from Ad-Ctrl sh and Ad-Tox4 sh treated mouse livers with 16-hr fast. Mice were sacced at Day 10 post adenovirus injection.