GSE106887
RNA sequencing of  human periodontal ligament stem cells (PDLSCs) with Illumina HiSeq2000
To study the molecular mechanisms underlying PDLSC osteogenic differentiation, we developed RNA sequencing with Illumina HiSeq2000 to comprehensively identify RNA expression profiles in normal PDLSCs, osteogenic inductive PDLSCs and mechanical force-induced PDLSCs. Plenty of RNAs were found to be differentially expressed by RNA sequencing. Furthermore, the potential functions of these RNAs were investigated using bioinformatic analysis. Overall design: The experiment included three samples: PDLSCs cultured with  normal media; PDLSCs cultured with osteogenic induction for seven days and PDLSCs stretched for 12h in a Flexcell® FX-5000™ Tension system.PDLSCs cultured with  normal media were considered as control group.