PRJNA717918
Microbial community and biosynthetic diversity exploration of soil metagenome using amplicon sequencing
Degenerate primers that recognize conserved regions in NRPS A domains (Pimentel-Elardo et al., 2012), PKSI KSI domains (Ginolhac et al., 2004) and 16S rRNA genes (Klindworth et al., 2013) were used to generate amplicons via PCR with isolated metagenomic DNA of the 7 soil samples from different sites and soil horizons of Schoenbuch forest in Tuebingen, Germany