GSE162254
Pooled CRISPR Screening Identifies m6A as a Positive Regulator of Macrophage Activation [m6A-RIP-seq]
Total RNAs were isolated from WT_CON, KO_CON, WT_LPS and KO_LPS Raw 264.7 cells, subjected to standard m6A RIP protocol. The libraries were generated with IPed and Input RNAs and subjected to Sequencing. Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat , and gene expression levels were measured by Cufflinks. Overall design: m6A modified RNAs were immunopriciptated and enriched with m6A speicifc antibody from WT_CON, KO_CON, WT_LPS and KO_LPS Raw 264.7 cells, and subjected to deep sequencing, in duplicate, using illumina HiSeq 2000.