ena-STUDY-BANGOR UNIVERSITY, MEFGL-15-10-2017-15:59:33:481-72
Comparing shotgun vs. amplicon sequencing analysis of environmental samples of invertebrates.
New applications of DNA and RNA sequencing are expanding the field of biodiversity discovery and ecological monitoring, yet questions remain regarding precision and efficiency. We used a set of documented mock communities comprising 13 species of freshwater macroinvertebrates of estimated individual biomass, to compare the detection efficiency of COI metabarcoding (3 different amplicons) and shotgun mito-metagenome sequencing. Additionally, we used individual COI barcoding and de novo mitogenome sequencing, to provide reference sequences for OTU assignment and mito-metagenome assembly respectively.
New applications of DNA and RNA sequencing are expanding the field of biodiversity discovery and ecological monitoring, yet questions remain regarding precision and efficiency. We used a set of documented mock communities comprising 13 species of freshwater macroinvertebrates of estimated individual biomass, to compare the detection efficiency of COI metabarcoding (3 different amplicons) and shotgun mito-metagenome sequencing. Additionally, we used individual COI barcoding and de novo mitogenome sequencing, to provide reference sequences for OTU assignment and mito-metagenome assembly respectively.