PRJNA540393
Clonal V?6+Vd4+ T cells promote IL-17-mediated immunity against Staphylococcus aureus skin infection
T cell cytokines contribute to immunity against Staphylococcus aureus, but the predominant T cell subsets involved are unclear. In a S. aureus skin infection mouse model, we found that the IL-17 response was mediated by ?d T cells, which trafficked from lymph nodes to the infected skin to induce neutrophil recruitment, proinflammatory cytokines IL-1a, IL-1ß and TNF and host defense peptides. RNA-seq for TRG and TRD sequences in lymph nodes and skin revealed a single clonotypic expansion of the encoded complementarity-determining region 3 amino acid sequence, which could be generated by canonical nucleotide sequences of TRGV5 or TRGV6 and TRDV4. However, only TRGV6 and TRDV4 but not TRDV5 sequences expanded. Finally, V?6+ T cells were predominant ?d T cell subset that produced IL 17A as well as IL-22, TNF and IFN?, indicating a broad and substantial role for clonal V?6+Vd4+ T cells in immunity against S. aureus skin infections.