GSE159855
Comprehensive analysis of metabolic and mitochondrial characteristics of extraembryonic endoderm cells
The purpose of the study was to identify transcriptomic changes between feeder-free ES and XEN cells. ES cells were cultured feeder-free on 0.1% gelatin in 2iLIF in N2B27 media. XEN cells were also cultured feeder-free on 0.1% gelaton  in RPMI1640 supplemented with 15% FBS, 2mM GlutaMAX and BME. Overall design: Bulk RNAseq of feeder-free ES and XEN cells under normal conditions