GSE135568
A multi-omics dataset of heat-shock response in the yeast RNA transport protein Mip6
We studied the heat-shock response in wild-type (wt) and in absence of Mip6, an RNA binding protein with roles at multiple levels of the gene expression pathway. This study include; their transcriptome (RNA-seq), metabolome (targeted NMR) and H4K12ac epigenome (ChIP-seq) using an experimental desing that ensures that the same samples are used across all omics determinations. This facilitates data analysis approaches that can leverage fully matched, longitudinal, replicated data. These data will faciliate studies of covariation patterns across multiple molecular layers controlling and responding to RNA biogenesis. Overall design: RNA-seq, histone H4 ChIP-seq and H4K12Ac ChIP seq of four biological replicates of wt and mip6D yeast cells growth at: (i) normal conditions; (ii) 20 minutes of heat shock at 39ºC and (iii) 120 minutes of heat shock at 39ºC.