GSE51273
The transcription factor Zbtb48 facilitate Polycomb recruitment at CpG islands and modulate PRC2 activity in mouse embryonic stem cells. [ChiP-Seq]
Genome-wide analysis of Zbtb48 and Polycomb binding in ctrl and Zbtb48 knock down mouse embryonic stem (mES) cells showed that Zbtb48 facilitated PRC1 and PRC2 recruitment to many CpG-islands. At bivalent CpG-islands Zbtb48 knock down led to an increase in K4me3 levels, and expression analysis using RNA-seq showed that neighboring genes were derepressed. In spite of reduced PRC1 and PRC2 levels we observed increased K27me3 levels at many loci, suggesting that ZBTB48 might modulate PRC2 activity. Overall design: Examination of Zbtb48 and Polycomb (Rnf2 and Suz12) binding, H3K4me3 and H3Ke27me3 levels, and gene expression mES cells that were transduced with Zbtb48 or control shRNA using ChIP- and RNA-sequencing.