69255e18-da2a-44b2-9a8a-4c2c03312aca
Gut microbiota profile on women with functional constipation
Introduction Functional constipation is caused by various factors, including luminal factors mediated by microbiota dysbiosis. The gut microbiota plays a fundamental role in several aspects of host health and disease. In this study, we aimed to uncover the effect of supplementation with an indigenous Lactiplantibacillus (formerly Lactobacillus) plantarum IS-10506 probiotic isolated from dadih on the composition and diversity of microbiota and the profile of short-chain fatty acids (SCFAs) in women with functional constipation compared with that in women without constipation. Methods A randomized, double-blind, placebo-controlled clinical trial was conducted on 37 women with functional constipation in Jakarta, Indonesia. The effect of the supplementation of 120 mL fermented milk containing the probiotic strain L. plantarum IS-10506 (1.2 × 1010 CFU/day) on the gut microbiota and SCFA profiles was compared with placebo and 10 non-constipation subjects, by using next-generation sequencing and gas chromatography–mass spectrometry, respectively. Results Baseline data showed dysbiosis of microbiota composition in terms of the Firmicutes/Bacteroidetes ratio and within the Clostridiales order; Ruminococcaceae and Mogibacteriaceae families; and Coprococcus, Prevotella, and Blautia genera. Women with constipation also had lower concentrations for acetate, propionate, and butyrate SCFAs compared with those without constipation. After 21 days of probiotic supplementation, a significant increase in Lactobacillacea abundance showed strong correlation with a significant increase in that of Lachnospiraceae, which in turn balanced the abundance of Ruminococcaceae sp., including Roseburia sp., and of Bilophila sp. The eubiotic tendency of the relative abundance of Roseburia sp. strongly correlated with the increasing SCFA concentration. SCFAs can also interact on the other side of the digestive tract, such as in the enteric nervous system, by stimulating motility to reliave functional constipation. Conclusion The supplementation of fermented milk containing L. plantarum IS-10506 (1.2 × 1010 CFU/day) of dadih origin improved the balance of microbiota toward eubiosis and increased SCFA concentration as underlying molecular mechanisms of relieving functional constipation symptoms in women.  Key words: Probiotic, Lactiplantibacillus plantarum IS-10506, functional constipation in women, microbiota profile, SCFA  Running title: Microbiota profile in functional constipation
Introduction Functional constipation is caused by various factors, including luminal factors mediated by microbiota dysbiosis. The gut microbiota plays a fundamental role in several aspects of host health and disease. In this study, we aimed to uncover the effect of supplementation with an indigenous Lactiplantibacillus (formerly Lactobacillus) plantarum IS-10506 probiotic isolated from dadih on the composition and diversity of microbiota and the profile of short-chain fatty acids (SCFAs) in women with functional constipation compared with that in women without constipation. Methods A randomized, double-blind, placebo-controlled clinical trial was conducted on 37 women with functional constipation in Jakarta, Indonesia. The effect of the supplementation of 120 mL fermented milk containing the probiotic strain L. plantarum IS-10506 (1.2 × 1010 CFU/day) on the gut microbiota and SCFA profiles was compared with placebo and 10 non-constipation subjects, by using next-generation sequencing and gas chromatography–mass spectrometry, respectively. Results Baseline data showed dysbiosis of microbiota composition in terms of the Firmicutes/Bacteroidetes ratio and within the Clostridiales order; Ruminococcaceae and Mogibacteriaceae families; and Coprococcus, Prevotella, and Blautia genera. Women with constipation also had lower concentrations for acetate, propionate, and butyrate SCFAs compared with those without constipation. After 21 days of probiotic supplementation, a significant increase in Lactobacillacea abundance showed strong correlation with a significant increase in that of Lachnospiraceae, which in turn balanced the abundance of Ruminococcaceae sp., including Roseburia sp., and of Bilophila sp. The eubiotic tendency of the relative abundance of Roseburia sp. strongly correlated with the increasing SCFA concentration. SCFAs can also interact on the other side of the digestive tract, such as in the enteric nervous system, by stimulating motility to reliave functional constipation. Conclusion The supplementation of fermented milk containing L. plantarum IS-10506 (1.2 × 1010 CFU/day) of dadih origin improved the balance of microbiota toward eubiosis and increased SCFA concentration as underlying molecular mechanisms of relieving functional constipation symptoms in women.  Key words: Probiotic, Lactiplantibacillus plantarum IS-10506, functional constipation in women, microbiota profile, SCFA  Running title: Microbiota profile in functional constipation