GSE185337
Comparative Transcriptomics Identifies Differentially Expressed Genes Regulating Gametic Development in Arabidopsis thaliana
Purpose:Comparative transcriptome analyses was applied to characterize gene expression during gametophytic development in Arabidopsis thaliana. Methods:  Leaf and immature floral buds (contain the entire developmental progress, > 0.4 mm long floral buds) were collected, then Separated male and female organs were kept in liquid nitrogen immediately until use. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results: In this study, both male and female gametes had much more similarity in gene expression patterns, particularly the genes involved in cell cycle and DNA repair, were enriched during the gametic development stage, but the differentially expressed genes related to DNA repair, recombination pathway  and genes in RdDM pathway. Conclusions: Genes encoding proteins associated with the production and modification of pectins and cell wall rearrangements were shown male-preferred implies the dynamic changes of pollen wall during the development of male gametes.   The DEGs, related to BR signaling pathway and BR synthesis pathway were highly enriched in developmental female gametes, suggesting that BR played pivotal roles in female gamete development. Overall design: mRNA profiles of male and female gametophytes