ena-STUDY-INSTITUTE OF CANCER RESEARCH-10-01-2018-10:23:28:085-1439
Focused PARP1 mutagenesis screen for talazoparib resistance in HeLa-PARP1-GFP and SUM149-GFP cells
HeLa-PARP1-GFP or SUM149-PARP1-GFP cells were mutagenised with pools of PARP1 CRISPR sgRNA vectors, selected in talazoparib, sorted for GFP expression and pooled RT-PCR products (reverse transcribed from GFP) from the resistant populations were sequenced.https://doi.org/10.1101/203224
HeLa-PARP1-GFP or SUM149-PARP1-GFP cells were mutagenised with pools of PARP1 CRISPR sgRNA vectors, selected in talazoparib, sorted for GFP expression and pooled RT-PCR products (reverse transcribed from GFP) from the resistant populations were sequenced.https://doi.org/10.1101/203224