GSE111532
Gene expression after depletion of fast cycling cells by heat shock, hydroxyurea, or colchicine treatment in the Sf-1 thermosensitive Hydra strain.
Treatments targetting fast cycling cells were applied to Hydra in order to: 1. identify compensatory gene expression behaviours after loss of neurogenesis, 2. improve the detection of cell-type expression for all transcripts in Hydra, together with the dataset entitled “Gene expressions in the three Hydra stem cell populations”. The processed data deposited here are also accessible in a graphical manner from a blast-based web interface available at https://hydratlas.unige.ch Overall design: Animals from the thermosensitive mutant strain Hydra Sf-1 (also called Hydra magnipapillata A9) were subjected to heat shock, hydroxyurea, or colchicine treatment and were sampled 9 (colchicine, heat shock) or 11 days (hydroxyurea) after the beginning of the treatment. In addition, animals treated with hydroxurea were sampled at 4, 5, and 7 days after the beginning of the treatment. Central body columns were used for sequencing. Most conditions are represented by 3 or 4 replicates. >> Grant Information << Awarded to Dr. Brigitte Galliot: - Swiss National Science Foundation (SNF grant 31003A-130337, 31003A_149630 & 31003_169930) - NCCR Frontiers in Genetics, Stem Cells & Regeneration project - The Canton of Geneva - The Claraz donation - Human Frontier Science Program (HFSP RHP0016-2010)