GSE137569
Generation of functional hepatocytes from extended pluripotent stem cells
We established efficiently differentiation protocol of human extented pluripotent stem (EPS) cells to functional hepatocytes (EPS-Heps) by simply adding a pre-treatment step before applying the directed differentiation protocol for conventional human pluripotent stem cells. Importantly, compared to hepatocytes derived from conventional human pluripotent stem cells, EPS-Heps transcriptionally more resemble primary human hepatocytes. Overall design: Examination of mRNA expression pattern of EPS cells, primed pluripotent stem cells, differentiated cells and freshly isolated fetal liver and freshly isolated primary hepatocytes (F-PHHs).