ena-STUDY-Glasgow Polyomics-16-08-2017-16:12:20:965-310
Host-associated niche metabolism enhances the expression of type 3 secretion and a co-ordinated suite of effector proteins during enteric infection
The integration of host-associated metabolism with bacterial virulence regulation dictates niche colonization by pathogens. Here, we employed in vivo RNA-seq to decipher the transcriptome of an attaching and effacing pathogen during infection. Citrobacter rodentium relies on a type 3 secretion system and a suite of effector proteins to colonize the host. The in vivo transcriptome was defined by the unique expression pattern of virulence factors essential for infection. We also describe the host-associated transcription of the operon for 1,2-propanediol metabolism, a microbiota fermentation product, and its role in enhancing expression of the type 3 secretion system via endogenous breakdown to propionate. Furthermore, we identified the co-ordinated expression profile of the effector repertoire in response to host signals such as 1,2-propanediol and reveal a novel function for one such effector in vivo. This study affirms the unprecedented power of in vivo transcriptomics for identifying novel links between host metabolism and virulence.
The integration of host-associated metabolism with bacterial virulence regulation dictates niche colonization by pathogens. Here, we employed in vivo RNA-seq to decipher the transcriptome of an attaching and effacing pathogen during infection. Citrobacter rodentium relies on a type 3 secretion system and a suite of effector proteins to colonize the host. The in vivo transcriptome was defined by the unique expression pattern of virulence factors essential for infection. We also describe the host-associated transcription of the operon for 1,2-propanediol metabolism, a microbiota fermentation product, and its role in enhancing expression of the type 3 secretion system via endogenous breakdown to propionate. Furthermore, we identified the co-ordinated expression profile of the effector repertoire in response to host signals such as 1,2-propanediol and reveal a novel function for one such effector in vivo. This study affirms the unprecedented power of in vivo transcriptomics for identifying novel links between host metabolism and virulence.