PRJNA866529
Ptms unlocks chromatin, Fig 7b, rat CNPase exon 1, CpG methylation (OPC x eGFP)
Brain glial progenitor cells in culture spontaneously transform into neoplastic cells with tumorigenic potential. Transcript analysis identified a histone binding protein up regulated during this process. An examination of its function when introduced into normal cells revealed a dramatic affects on chromatin, with decreased DNA methylation and induction of glioma identity genes.SUUB11896962 contains individual dna.seq (fasta format) of the CNPase exon 1 in rat brain glial cells. Cells were transfected with a control (eGFP) vector then treated with Ct conversion reagent (Zymo Research, Cat #D5001-1) to distinguish methylated and non-methyl CpG sites.