GSE157637
Single cell Cut&Tag in the mouse brain
We performed single cell Cut&Tag using 10x genomics chromium platform with antibodies agains H3K4me3, H3K27ac, H3K27me3, H3K36me3, Rad21 and Olig2 on nuclei isolated from the mouse brain. The cells were sorted based on GFP fluorescence, from Sox10-Cre/GFP whole mouse brain at postnatal day P21-25. Additionaly, H3K4m3 and H3K27me3 scC&T includes both Sox10-Cre/GFP positive and negative cells sorted from the whole mouse brain at postnatal day P15. Overall design: Cut&Tag profiles were generated by antibody directed tagmentation, coupled to standart 10x chromium ATAC-seq protocol. Single cell libraries were sequenced on NovaSeq platform and aligned and processed using cellranger-atac 1.2.0. Data was aggregated into cell by bin matrices and analyzed with R packages Seurat/Signac.