GSE202704
snRNA-seq on hippocampus (Hip) and prefrontal lobe cortex (PFC) tissue in mice
We performed high-throughput snRNA-seq on hippocampus (Hip) and prefrontal lobe cortex (PFC) tissue in mice (Mus musculus) to identify cell-type specific differentially expressed genes. Mice were divided into GF, SPF and CGF group. Overall design: 10X Genomics Chromium snRNA-seq was performed on nuclei extracted from Hip and PFC of 3 GF mice, 3 SPF mice and 3 CGF mice at 8 week old. Cell-types were identified by unsupervised clustering and diffrential gene expression was performed between cases and controls for each cell-type cluster. The library preparation was performed with the Chromium Singel Cell 3' Reagent Kits v2. Paired-end sequencing was performed on theIllumina NovaSeq 6000. FastQ files were produced with Cellranger v2.1.0 mkfastq. Alignments and gene counts were generated with Cellranger v2.1.0 count.