E-MTAB-10508
RNA-seq of mouse neutrophils polarized to proinflammatory N1 by lipopolysaccharide and interferon-? or anti-inflammatory N2 by interleukin-4 versus control
Gene expression profiling for mouse N1 and N2 neutrophils. Neutrphils were isolated form the bone marrow of C57BL/6J mice between 12-16?weeks old. Approximately 8x10^7 neutrophils were used per condition and were split into 3 biological replicates.  Freshly isolated neutrophils were cultured for 2h in RPMI medium, in the presence of 100 ng/ml lipopolysaccharide (LPS) and 20 ng/ml interferon gamma (IFN?) or 20 ng/ml interleukin 4 (IL-4). Total RNA was isolated with TRIzol reagent and Phasemaker tubes. A sample from the LPS+IFN? polarization did not pass the quality control test and was excluded from the downstream analysis.
Gene expression profiling for mouse N1 and N2 neutrophils. Neutrphils were isolated form the bone marrow of C57BL/6J mice between 12-16?weeks old. Approximately 8x10^7 neutrophils were used per condition and were split into 3 biological replicates.  Freshly isolated neutrophils were cultured for 2h in RPMI medium, in the presence of 100 ng/ml lipopolysaccharide (LPS) and 20 ng/ml interferon gamma (IFN?) or 20 ng/ml interleukin 4 (IL-4). Total RNA was isolated with TRIzol reagent and Phasemaker tubes. A sample from the LPS+IFN? polarization did not pass the quality control test and was excluded from the downstream analysis.