PRJNA834901
Gut metagenome
The microbial community DNA was extracted using the MagPure Stool DNA KF kit B following the manufacturer's instructions. Variable regions V3V4 of bacterial 16S rRNA gene was amplified with degenerate PCR primers. Gut microbiota composition was assessed using Illumina MiSeq platform and QIIME-based microbiota analysis (This method is described in detail in the Supplementary information).