Rat genome resequencing
Comparative analysis of two rat inbred lines
Here we re-sequenced two rat inbred genomes in Illumina platform, which the depth is as high as 35X. We used the BN genome as the reference to map all reads to it and the coverage is 90% or more. Based on alignment, we identified SNPs, InDels, SVs, CNVs for each strain. Then we also validate our SNPs and/or InDels by using Sanger sequencing, which the accurate rate reached up to 94.75%. According these data, we constructed two detailed genetic variation maps. Finally, we also assembly a draft genome for each line combining concensus of themselves based on these resequencing data. These results will support our following medical research dramatically.