GSE130151
Single-cell sequencing of Klinefelter testicular biopsies
We have evaluated the transcriptome of different testicular cell types, including germ cells, at single cell level in a testicular biopsy of a man with Klinefelter syndrome (XXY karyotype) Overall design: A testicular biopsy was enzymatically digested and single-cell RNAseq libraries were obtained using 10X genomics platform (10x Genomics Chromium Single Cell Reagent Kit v2). The libraries were sequenced on a NovaSeq600 sequencer (Illumina) using 2x150 bp paired-end sequencing.