GSE19476
A rapid and scalable system for studying gene function in mice using conditional RNA interference
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luciferase miRNA is expressed as the 8th most abundant species in the cells. Keyword(s): Epigenetics Overall design: 4 libraries were analyzed