RNAseq_of_mouse_POMC_neurons-sc-3464
RNAseq_of_mouse_POMC_neurons
From the arcuate nucleus of mice, we have dissociated neurons that express Proopiomelanocortin (POMC) and captured them on the Fluidigm C1 platform. These neurons play important roles in the regulation of appetite and sexual behavior. Here we propose to sequence RNA from 96 single POMC-expressing neurons to understand the variance in their transcriptomes.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Neurons that express pro-opiomelanocortin (POMC), were dissociated from the arcuate nucleus of mice. The cells were isolated by FACS, then 96 single neurons were captured on the Fluidigm C1 platform. The RNA from these were sequenced on the Illumina Hiseq platform.