PRJNA701150
Cucumis sativus cultivar:KS Raw sequence reads
Total RNA was extracted from CGMMV-infected cucumber plants cv. KS. Leavesfrom two plants exhibiting either the early or the late post-recovery stage symptoms(two plants per each stage) and two un-inoculated control plants were subjected todissections of uniform discs, 3mm in diameter, from either the newly identified brightyellow islands (see Results) or the corresponding dark surrounding tissues, in thesymptomatic plants, and parallel dissections were performed on the control un238inoculated plants. Total RNA was extracted from three combined discs, from each ofthe tested symptomatic features as well as the un-inoculated controls, using theGenEluteTM Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to themanufacturer's instructions. Discs were ground in the lysis buffer using plastic sticks in1.7ml Eppendorf vials. Total RNA was eluted into RNase free double-distilled water(DDW); DNA was removed using the DNA-freeTM DNA Removal Kit (Thermo FisherScientific, USA) according to the manufacturer's instructions and stored at -80 Celsius. Theextracted RNA served for library constructions using the TruSeq RNA Library Prep(Illumina) commercial kit. RNA sequences containing poly-A tails were enriched usingbeads containing oligo-dT primers. The enriched RNA was used for constructions ofcDNA libraries using random hexamers