PRJNA553644
Arabidopsis thaliana Raw sequence reads
Poly(A) tail length (PAL) has been implicated in the regulation of mRNA activities. However, the extent of such regulation at the transcriptome level is less understood in plants. Herein, we report the development and optimization of a large-scale sequencing technique called Assay for PAL-sequencing (APAL-seq). To explore the role of PAL in post-transcriptional modification and translation, we performed PAL profiling of Arabidopsis in response to heat shock. Our results suggest that plant genes have a high variability of PALs and it contributes to swift posttranslational stress response.