GSE161317
T-5224, a selective inhibitor of c-Fos/AP-1, suppresses IgE-mediated mast cell activation†
Purpose:The goal of this study is to asses the gene expression changes  in  non-stimulated  mast  cell and  anti-IgE-stimulated mast cell Methods:RBL-2H3 mRNA profiles of non-stimulated  mast  cell and  anti-IgE-stimulated mast cell were generated by deep sequencing, in triplicate. Differential expression analysis was performed using the DESeq2(v1.4.5) with Q value ?0.05, following the heatmap was drawn by pheatmap(v1.0.8) . Results:Compared with non-stimulated mast cell, anti-IgE-stimulated mast cell induced a robust  transcriptional response, with 191 differentially expressed genes following anti-IgE treatment (116 upregulated and 75 downregulated). Among the up-regulated genes. Conclusions:Our study represents the first detailed analysis of RBL-2H3 transcriptomes, with biologic replicates, generated by RNA-seq technology. Overall design: RBL-2H3 mRNA profiles of   non-stimulated  mast  cell and  anti-IgE-stimulated mast cell