AHQL1: Arabidopsis polyadenylation sites
The genome-wide landscape of polyadenylation in Arabidopsis:  evidence for extensive and novel alternative polyadenylation
This project entails the systematic characterization of poly(A) site choice in Arabidopsis leaves and seeds.  Custom-made cDNA tags that query the mRNA-poly(A) junction were prepared and sequenced on the Illumina GAII platform.  Thee tags were then curated and mapped to the Arabidopsis genome.  From these studies, a global overview of poly(A) site choice has been obtained, and numerous instances of alternative polyadenylation and antisense transcription identified.