Mutational_Signatures_in_DNA_repair_KO_cells-sc-4965
Mutational_Signatures_in_DNA_repair_KO_cells
Looking at mutational signatures in an isogenic cell line background
This project is being carried out through a Strategic Award from the Wellcome Trust to Steve Jackson, Mike Stratton, Peter Campbell, Serena Nik-Zainal and others.  This grant is to fund work aimed at identifying “mutational signatures” arising through cells being defective in DNA repair and other cellular factors, and/or through cellular exposure to various DNA damaging agents.   The samples that are here being provided here are from human retinal pigmented epithelium cell culture, and represent wild-type cells plus various gene knock-out cells that have or have not been propagated over several months to acquire mutations.  The samples are to be subject to whole-genome sequencing. Ensuing sequence data will be subject to bioinformatic analyses to identify mutation signatures and their underlying causes.