ena-STUDY-KING'S COLLEGE LONDON-24-05-2019-08:55:26:649-305
Whole shotgun metagenomic sequencing for 1,004 individuals belonging to the TwinsUK cohort.  Supporting data for: Visconti et al, Interplay between the human gut microbiome and host metabolism
Nextera XT libraries were prepared manually following the manufacturers' protocol (Illumina, PN. 15031942). Briefly, samples were normalized to 0.2 ng/ml DNA material per library using a Quant-iT picogreen assay system (Life Technologies, PN. Q33120) on an AF2200 plate reader (Eppendorf), then fragmented and tagged via tagmentation. Amplification was performed by Veriti 96 well PCR (Applied Biosystems) followed by AMPure XP bead cleanup (Beckman Coulter, PN. A63880). Fragment size for all libraries was measured using a Labchip GX Touch HiSens. Sequencing was performed on an Illumina HiSeq 2500 using SBS kit V4 Chemistry, with a read length of 2x125 bp.  Metadata on TwinsUK twin participants are available to bona fide researchers under managed access due to governance and ethical constraints. Raw data should be requested via our website (http://twinsuk.ac.uk/resources-for-researchers/access-our-data/) and requests are reviewed by the TwinsUK Resource Executive Committee (TREC) regularly.
Nextera XT libraries were prepared manually following the manufacturers' protocol (Illumina, PN. 15031942). Briefly, samples were normalized to 0.2 ng/ml DNA material per library using a Quant-iT picogreen assay system (Life Technologies, PN. Q33120) on an AF2200 plate reader (Eppendorf), then fragmented and tagged via tagmentation. Amplification was performed by Veriti 96 well PCR (Applied Biosystems) followed by AMPure XP bead cleanup (Beckman Coulter, PN. A63880). Fragment size for all libraries was measured using a Labchip GX Touch HiSens. Sequencing was performed on an Illumina HiSeq 2500 using SBS kit V4 Chemistry, with a read length of 2x125 bp.  Metadata on TwinsUK twin participants are available to bona fide researchers under managed access due to governance and ethical constraints. Raw data should be requested via our website (http://twinsuk.ac.uk/resources-for-researchers/access-our-data/) and requests are reviewed by the TwinsUK Resource Executive Committee (TREC) regularly.