E-MTAB-11895
Bulk RNA-sequencing of mRNA from Sertoli cells isolated by a novel single cell suspension protocol and FACS-sorting
Analysis of bulk-mRNA from Sertoli cells of pre-puberal, puberal and young adult mice. We have developed a novel method for preparation of the single cell suspension from seminiferous tubules and subsequent Sertoli-cell specific staining and FACS-sorting, which takes less than 2 hours.
Analysis of bulk-mRNA from Sertoli cells of pre-puberal, puberal and young adult mice. We have developed a novel method for preparation of the single cell suspension from seminiferous tubules and subsequent Sertoli-cell specific staining and FACS-sorting, which takes less than 2 hours.