E-MTAB-2044
ChIP-Seq of OCT4, NANOG, p300 in 3iL Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts.  The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine.  Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. This experiment is the transcription factor binding data of the study.
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts.  The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine.  Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. This experiment is the transcription factor binding data of the study.