ena-STUDY-Hebei University of Technology-23-10-2018-08:14:03:513-240
Here we report on the metagenome sequence data from a microbial community associated with natural crude oil seeps of the Bohai Gulf, one of China's largest natural hydrocarbon seep regions, which can be exploited for further analysis for oil-spill bioremediation.
The seawater sample of a hydrocarbon-adapted microbial consortium was collected using a electromotion aspiring pump from the seabed of an oil drilling platform located near at N38°26'31.7?, W118°24'45.2?. 20L seawater sample was filtered to hydrophilic polyethersulfone filter membrane (0.22µm pore size, 47 mm diameter, Merck KGaA, Darmstadt, Germany), transported immediately to the laboratory at -40°C and stored at -70°C until DNA extraction was performed. Purified DNA was submitted to Illumina HiSeq 2500 for PE150 sequencing. DNA was extracted with MO BIO PowerSoil® DNA Isolation Kit (MO BIO Laboratories, Inc. Carlsbad, CA).
The seawater sample of a hydrocarbon-adapted microbial consortium was collected using a electromotion aspiring pump from the seabed of an oil drilling platform located near at N38°26'31.7?, W118°24'45.2?. 20L seawater sample was filtered to hydrophilic polyethersulfone filter membrane (0.22µm pore size, 47 mm diameter, Merck KGaA, Darmstadt, Germany), transported immediately to the laboratory at -40°C and stored at -70°C until DNA extraction was performed. Purified DNA was submitted to Illumina HiSeq 2500 for PE150 sequencing. DNA was extracted with MO BIO PowerSoil® DNA Isolation Kit (MO BIO Laboratories, Inc. Carlsbad, CA).