PRJNA731624
Raw sequence reads from healthy human blood samples
The protocol used in this study intended to enrich viral fraction in order to characterize human virome from healthy blood donors using plasma fraction. A first centrifugation in order to obtain plasma fraction was performed. Plasma was filtered through filters with different pore size (0.45 and 1 micrometers) to remove cells and other non-viral particles. After that, 1 mL of filtered plasma was mixed in pools of 10 different samples. Each pool was subjected to high-speed centrifugation and resuspended in digestion buffer. Then, nucleases were added in order to remove unprotected nucleic acids. After incubation, stop reagent was added. Then, viral DNA and RNA extraction were performed, followed by a random amplification and library preparation.