ena-STUDY-QMUL-17-05-2018-16:45:50:804-192
Illumina RAD-seq reads for 190 dwarf birch (Betula nana) individuals in 36 populations in Scotland and Finland
We extracted DNA from 190 dwarf birch (Betula nana) individuals from 36 populations in Scotland and Finland. We used the restriction enzyme PstI. Cut DNA was normalized and submitted to FLORAGENEX (Portland, Oregon) for generation and sequencing of RAD tags following the protocol of Baird et al. (2008) and Hohenlohe et al. (2010). In brief, libraries were prepared with 96 unique 8-base barcodes, ligated via adaptors to PstI digested genomic DNA. The resulting fragments were sequenced in two lanes of an Illumina GAIIx platform with single-end 1x100bp chemistry. A single internal control sample generated from Saccharomyces bayanus was included in each lane. To match each set of reads with an individual and location, look at the "Sample title" column. The first two letters of each “Sample Title” correspond to population names and locations listed in Table S2 of Borrell et al 2018 Heredity
We extracted DNA from 190 dwarf birch (Betula nana) individuals from 36 populations in Scotland and Finland. We used the restriction enzyme PstI. Cut DNA was normalized and submitted to FLORAGENEX (Portland, Oregon) for generation and sequencing of RAD tags following the protocol of Baird et al. (2008) and Hohenlohe et al. (2010). In brief, libraries were prepared with 96 unique 8-base barcodes, ligated via adaptors to PstI digested genomic DNA. The resulting fragments were sequenced in two lanes of an Illumina GAIIx platform with single-end 1x100bp chemistry. A single internal control sample generated from Saccharomyces bayanus was included in each lane. To match each set of reads with an individual and location, look at the "Sample title" column. The first two letters of each “Sample Title” correspond to population names and locations listed in Table S2 of Borrell et al 2018 Heredity