E-MTAB-11647
Sequences that influence mRNA trans splicing and polyadenylation in Trypanosoma brucei
Trypanosoma brucei library consisting of  a pool of reporters whose 14Ts polypyrimidine tract  is replaced with 11 random nucleotides. The library is treated with puromycin at a concentration of either 0.2ug/ml, 0.4ug/ml or 1ug/ml.   The reporters are recovered and PCR amplicons targeting the random sequences identified by HTS. The results are paired reads, where the suffixes _1  and _2 are reads 1 and 2 respectively. Also included are sequencing results from the plasmid library.
Trypanosoma brucei library consisting of  a pool of reporters whose 14Ts polypyrimidine tract  is replaced with 11 random nucleotides. The library is treated with puromycin at a concentration of either 0.2ug/ml, 0.4ug/ml or 1ug/ml.   The reporters are recovered and PCR amplicons targeting the random sequences identified by HTS. The results are paired reads, where the suffixes _1  and _2 are reads 1 and 2 respectively. Also included are sequencing results from the plasmid library.