GSE183016
polysome profiling RNA seq experiments on extracts from METTL3-WT and METTL3-K177Q reconstituting cells
Given the overall steady-state mRNA abundance between these two groups displayed negligible differences, these data implied an increased translational efficiency in cells bearing K177Q mutation. Overall design: Using sucrose gradient centrifugation, collected fractions were pulled into sub-polysome fraction and polysome fraction. Then, sub-polysome and polysome samples were subjected to RNA-seq.