GSE126584
Escherichia coli DH5alpha Transcriptome Sequencing  under Wi-Fi radiofrequency radiation
Purpose:  In this study, Escherichia coli DH5alpha whole transcriptome sequencing was performed in order to compare the different gene expression profiles between control and  exposed  to Wi-Fi radiofrequency radiations. Methods:Escherichia coli DH5alpha were exposed to Wi-Fi radiations. Total RNA samples( control and exposed ) were extracted by bacteria protect-Rneasy kit,treated with DNAase and subjected to sequnecing using an Illumina-NovaSeq 6000 platform. Library preparation and sequencing were performed by Macrogen (south korea).Trimmed reads are mapped to reference genome with Bowtie. HTseq was used for expression profiling. Expression profile was calculated for each sample and gene as read count. Overall design: 6 samples at mid log phase including control and Wi-Fi exposed E.coli DH5a for 5 hours, in triplicate, were analyzed using Illumina NovSeq6000 plateform