GSE135390
RNA-seq of human CD4+ T cell subsets
After activation, CD4+ T helper (Th) cells differentiate into functionally specialized populations that coordinate distinct immune responses and protect against different types of pathogens.  In humans, these effector and memory Th cell subsets can be readily identified in peripheral blood based on their differential expression of chemokine receptors that govern their homeostatic and inflammatory trafficking.  Foxp3+ regulatory T (Treg) cells can also be divided into subsets that phenotypically mirror each of these effector populations, and share expression of key transcription factors and effector cytokines.  In this study, we performed comprehensive transcriptional profiling of 11 phenotypically distinct Th and Treg cell subsets sorted from peripheral blood of healthy individuals.  Despite their shared phenotypes, we found that mirror Th and Treg subsets were transcriptionally dissimilar, and that Treg cell populations showed limited transcriptional diversity compared to Th cells. We identified core transcriptional signatures shared across all Th and Treg cell populations, and unique signatures that define each of the Th or Treg populations.  Finally, we applied these signatures to bulk Th and Treg RNA-seq data and found enrichment of specific Th and Treg cell populations in different human tissues.  These results further define the molecular basis for the functional specialization and differentiation of Th and Treg cell populations, and provide a new resource for examining Th and Treg specialization in RNA-seq data. Overall design: Transcriptional analysis of 11 different CD4+ T cell populations from 3 separate donors 11 CD4+ T cell populations were sorted from the blood of 3 individual donors as follows: Naïve (CD127+CD25-CD45RA+), Th1 (CD127+CD25-CD45RA-CXCR3+CCR6-), Th2 (CD127+CD25-CD45RA-CXCR3-CCR6-CCR4+), Th17 (CD127+CD25-CD45RA-CXCR3-CCR6+CCR4+CCR10-), Th1/17 (CD127+CD25-CD45RA-CXCR3+CCR6+), Th22 (CD127+CD25-CD45RA-CXCR3-CCR6+CCR4+CCR10+), Treg1 (CD127-CD25+CD45RA-CXCR3+CCR6-), Treg2 (CD127-CD25+CD45RA-CXCR3-CCR6-CCR4+), Treg17 (CD127-CD25+CD45RA-CXCR3-CCR6+CCR4+CCR10-), Treg 1/17 (CD127-CD25+CD45RA-CXCR3+CCR6+), Treg 22 (CD127-CD25+CD45RA-CXCR3-CCR6+CCR4+CCR10+)