ena-STUDY-UNIVERSITY OF EDINBURGH-05-12-2020-13:29:00:001-179
Chromium single cell transcriptomics of wild-type and ZC3H20 KO trypanosoma brucei undergoing oligopeptide induced differentiation
wild-type and ZC3H20 null Antat 1.1 90:30 trypanosome brucei were exposed to brain heart infusion broth to induce differerntiation from slender forms. Slender, stumpy and transitioning cells were mixed into one heterogenous population before 15,000 parasites were subject to scRNA-seq with Chomium 3' expression v3 chemistry. Reads were mapped to the TREU927 genome plus maxi circle kDNA sequence.
wild-type and ZC3H20 null Antat 1.1 90:30 trypanosome brucei were exposed to brain heart infusion broth to induce differerntiation from slender forms. Slender, stumpy and transitioning cells were mixed into one heterogenous population before 15,000 parasites were subject to scRNA-seq with Chomium 3' expression v3 chemistry. Reads were mapped to the TREU927 genome plus maxi circle kDNA sequence.