ena-STUDY-ENS Lyon-13-03-2017-10:51:19:908-340
Mapping-by-sequencing data for mutant allele mf86 (Oscheius tipulae)
The Oscheius tipulae strain JU163 carries the recessive mutation mf86 in the CEW1 background (reference genome). mf86 is an allele of the locus iov-1, published in Dichtel-Danjoy et al., 2004 (PMID:14697357)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 153 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.
The Oscheius tipulae strain JU163 carries the recessive mutation mf86 in the CEW1 background (reference genome). mf86 is an allele of the locus iov-1, published in Dichtel-Danjoy et al., 2004 (PMID:14697357)In order to map and identify this mutation, it was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from 153 independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.