PRJNA433717
Methanol-free Pichia pastoris for protein expression
The use of Pichia pastoris heterologous expression system is restricted due to limitations on the use of methanol. By deleting transcription repressors and overexpressing transcription activator of the aldehyde oxidase 1 (AOX1) promoter, a methanol-independent strain ?mig1?mig2?nrg1-Mit1 has been previously constructed. This study aimed to investigate the transcriptome and metabolome profile of the strain in response to different carbon sources.