GSE127864
Small RNA sequencing of mouse bone marrow derived extracellular vesicles [EV.sRNA]
Mouse BM extracellular vesicles were isolated from Ocn-GFP Topaz mice using the Exoeasy and miRneasy (Qiagen). Small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England biolabs). Libraries were sequenced using Hiseq2500 instrument Overall design: Small RNA libraries were constructed from total RNA extracted from mouse bone marrow derived extracellular vesicles