Comparative_Genomics_of_Microsporidia
Comparative Genomics of Microsporidia
The project is to sequence 13 species/isolates from the Microsporidia community.  The proposed sequencing model uses 454 single reads to generate contigs and 454 paired-end reads from a jumping library to link these into larger scaffolds.  3kb jumps will be used to generate 15-fold coverage in 454 fragment reads and 30-fold physical coverage in paired 454 reads.  Each genome will require 1/2 to one full 454 sequencing run.  The Microsporidia are:  Encephalitozoon cuniculi Anncallia algerae Edhazardia aedis Enterocytozoon bieneusi Nematocida parisii Nucleospora salmonis Pseudoloma neurophilia Paranucleospora theridion Vavraia culicis  Vittaforma corneae