GSE185637
Single cell RNAseq data from Dlk1+ and Dlk1- aged murine hematopoietic stem cells
Quantification of gene expression noise on young versus aged bone marrow HSCs at single-cell resolution revealed Dlk1 increases both noise and expression levels upon aging. Dlk1, encoding a cell surface non-canonical Notch ligand, is partially expressed in the LT-HSC compartment, without generating clusters of individual subpopulations by conventional clustering analysis. We aimed to characterize the trascriptome of aged LT-HSC with positive and negative expression of Dlk1 cells. Dataset created as part of the publication: "VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing  hematopoietic stem cells" Overall design: LT-HSC (Lin-CD150+CD48-CD34-Kit+Sca1+) Dlk1+ and Dlk1- cells from bone marrow of 18 months old mice were sorted into 384-well plates. Cells processed for scRNAseq with a similar mCELseq2 protocol (Herman et al., 2018 Nat Methods, DOI: 10.1038/nmeth.4662)