E-MTAB-8580
RNA-seq of wild-type and transformed Ink4a/Arf-/-; EGFRvIII neural stem cells in separate or co-culture conditions
There is increasing evidence demonstrating that adult neural stem cells (NSCs) are the cell of origin of glioblastoma (GBM), the most aggressive subtype of malignant glioma. The earliest stages of hyperplasia are challenging to explore, but likely involve a cross-talk between normal and transformed NSCs. How normal cells respond to this cross-talk and what impact this has on the NSC activation/quiescence balance is poorly understood. We sought to address this question by analysing how transformed and wild-type NSCs isolated from the mouse stem cell niche in the subventricular zone (SVZ) interact. Wild-type and Ink4a/Arf-/-; EGFRvIII transformed NSCs were cultured either separately or in a 50:50 co-culture in serum-free adherent conditions. We observed that the growth of wild-type NSCs was dramatically reduced in the presence of the transformed NSCs. To explore this finding further we performed RNA-seq on NSCs that had been sorted by FACS following five days of culture either separately or in the co-culture condition. This allowed us to identify differentially expressed genes between the different culture conditions.
There is increasing evidence demonstrating that adult neural stem cells (NSCs) are the cell of origin of glioblastoma (GBM), the most aggressive subtype of malignant glioma. The earliest stages of hyperplasia are challenging to explore, but likely involve a cross-talk between normal and transformed NSCs. How normal cells respond to this cross-talk and what impact this has on the NSC activation/quiescence balance is poorly understood. We sought to address this question by analysing how transformed and wild-type NSCs isolated from the mouse stem cell niche in the subventricular zone (SVZ) interact. Wild-type and Ink4a/Arf-/-; EGFRvIII transformed NSCs were cultured either separately or in a 50:50 co-culture in serum-free adherent conditions. We observed that the growth of wild-type NSCs was dramatically reduced in the presence of the transformed NSCs. To explore this finding further we performed RNA-seq on NSCs that had been sorted by FACS following five days of culture either separately or in the co-culture condition. This allowed us to identify differentially expressed genes between the different culture conditions.