DRP006741
Genome-wide analysis of cleavage sites in fruit flies (Drosophila melanogaster)
In previous studies, high-throughput approaches for genome-wide profiling of RNA cleavage sites were developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3' transcripts ; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) using D. melanogaster (S2-R+ cells) in exponential growth phase to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.