GSE40997
Transcriptome analysis of Dunaliella viridis in response to changes in light duration and temperature
Transcriptome analysis of Dunaliella viridis in response to changes in light duration and temperature Overall design: Time course experiment  experiment with 5 evenly spaced time points, t1-t5, and two experimental factors: a) light condition (2 levels: continuous light and light/dark cycle) and b) temperature (2 levels: 25 degrees Celsius and 35 degrees Celsius). Cells grown in the light/dark condition were exposed to a 12 hour light/dark cycle, whereas continuous light samples were grown under constant light conditions.  All cells were grown at 25 degrees Celsius for timepoints t1 and t2; at time t3, cells grown in the high temperature condition were raised to 35°C, and kept at this temperature for the remainder of the experiment.  Bar-coded samples were sequenced in two separate sequencing runs using 100-nt Illumina RNA-Seq reads. In the first run a single pooled biological replicate was sequenced from each of the 12 combinations of time, light condition and temperature from time points t3 to t5. The bar-coded samples were divided among 4 Illumina Lanes resulting in 48 distinct sequencing data files. In the second run ("Run 2") a single bar-coded biological replicate was sequenced from each of the 10 combinations of time and light condition from time points t1 through t5, with temperature fixed at 25°C.