decode and decide profile
Global fitness profiling of fission yeast deletion strains by barcode
The raw data used to decode the barcode of deletion library and use the decoded barcode to research on the global fitness profiling of fission yeast deletion strains
A genome-wide deletion library is a powerful tool for probing gene functions and   it has recently become available for the fission yeast Schizosaccharomyces pombe. Here   we present a high-throughput method for quantitatively measuring the fitness of fission   yeast deletion strains by deep sequencing of the DNA barcodes built into each strain. In   this method, thousands of strains are pooled together in one culture and then subject to   various treatments. The fitness phenotypes of pooled strains are analyzed in parallel in   one sequencing reaction. The throughput and cost-effectiveness of the assay are further   improved by a multiplexed sequencing strategy. The barcode sequencing data showed   good reproducibility and linearity, and we validated the use of barcode sequencing for   fitness analysis by detecting auxotrophic mutants that failed to grow in a minimal   medium. We applied barcode sequencing to profile the fitness changes of mutants upon   treatment with three types of genotoxins and an anti-microtubule compound   thiabendazole (TBZ). More than two hundred mutants hypersensitive to at least one   treatment were identified. Genes with known functions in DNA damage response and   mitosis were highly enriched among the hypersensitive hits. Unexpectedly, besides   sensitive mutants, fitness profiling also revealed mutants resistant to drug treatments,   including several mutants resistant to anticancer drug camptothecin (CPT). Finally, as a   demonstration of the use of barcode sequencing in revealing new gene functions, we   report the identification of a previously uncharacterized gene required for centromere   silencing.