GSE161696
RPL8 extensively regulates the expression and alternative splicing of cancer-related genes
Objective: In our study, we aimed to explore the molecular mechanism of RPL8 in tumors by analyzing over-expression of RPL8  in  HeLa  cells.   Method: Two experimental  groups and control groups were setup. The experimental groups adopted Hela cells transfected by RPL8 plasmid ,  and  the  control  group  used  the  Hela  cells  transfected by blank  plasmid. The  total  mRNA  of  the  cells  were extracted  after  transfection for 48  hours and the expression  level  of  mRNA was detected by using high-throughput  sequencing  technology.  Then R Bioconductor  software  package  edgeR  was  used  to  screen  differentially  expressed genes and TopHat2 was used to analyze the splice site of each sample. Finally, GO and KEGG  analysis  of  differentially  expressed  genes  and  differential  alternative splicing events were performed, and the top ten functional pathways were selected for  display.    Results: Overexpression  of  RPL8  produced  a  large  number  of differentially expressed genes and differential alternative splicing. Many of these differentially  expressed  genes  and  differential  alternative  splicing  genes were enriched in tumor-related pathways, including inflammation, positive regulation of cell proliferation , Angiogenesis, etc. In addition, many of these differentially expressed  genes  have  been  shown  to  be  closely  related  to  the  occurrence  and development  of  tumors.    Conclusion: RPL8  can  widely  regulate  the  expression  and alternative splicing of tumor-related genes. Overall design: Detecting the expression level of differential genes and alternative splicing after RPL8 overexpression in HeLa cells,