GSE121995
H1609088 Human RNA-Sequencing
Transcriptome profiling of KYSE30 cells after intracellular invasion by Porphyromonas gingivalis  Purpose: Overabundance of Porphyromonas gingivalis (P. gingivalis) plays oncogenic roles in development and progression of esophageal squamous cell carcinoma (ESCC). To unveil the molecular mechanisms underlying the tumor-promoting role, RNAseq was used to identify differentially expressed genes in response to P. gingivalis in KYSE30 cells.  Methods: mRNA profiles were generated by deep sequencing for P. gingivalis-treated KYSE30 cells and PBS-treated control cells in triplicate. RNAseq data were mapped to human genome (hg19) using Hisat2. Differentially expressed genes were identified using StringTiel and Ballgown.  Results: A total of 10 239 genes and a total of 10 297 genes were identified in KYSE30 cells treated with P. gingivalis and PBS, respectively. After expression quantification using Ballgown, 124 up-regulated genes and 122 down-regulated genes were identified in P. gingivalis-treated KYSE30 cells compared with PBS-treated cells. Enrichment analysis of GO and KEGG pathway showed response to virus, innate immune response, Herpes simplex infection, metabolism of xenobiotics by cytochrome P450 and tyrosine metabolism were enriched in P. gingivalis-treated KYSE30 cells.  Conclusions: This study reveals that P. gingivalis infection in ESCC triggers immune response and may cause deregulation of signal transduction through tyrosine metabolism. Overall design: mRNA profiles were generated by deep sequencing for P. gingivalis-treated KYSE30 cells and PBS-treated control cells in triplicate. RNAseq data were mapped to human genome (hg19) using Hisat2. Differentially expressed genes were identified using StringTiel and Ballgown.