GSE183552
The effect of 22-bulge of pre-miRNAs on DICER cleavage in human cells
The experiments were conducted to examine the effects of 22-bulge of pre-miRNAs on DICER cleavage in human cells. Overall design: HCT116 cells were transfected with pCDNA3 empty, pCDNA3 wild-type or SNP-containing pri-miRNA plasmids. Small RNA sequencing libraries of the transfected HCT116 cells generated using NEBNext® Small RNA Library Prep Set for Illumina® (NEB).