PRJNA448735
Tolypocladium inflatum Genome sequencing and assembly
This bioproject sequenced six isolates of the cyclosporin producing fungus Tolypocladium inflatum using PacBio next generation sequencing and Illumina technology to generate near chromosomal level assemblies of all strains in order to address the role of genome location and rearrangements in generating intraspecific diversity of secondary metabolite genes and clusters. These strains come from distinct geographic locations and environments and represent a sample of the diversity of this cosmopolitan species. Each strain was sequenced with PacBio and assembled denovo with the HGAP3 assembler. Illumina reads (~50-80x coverage) were used for error correction of the PacBio reads using Pilon. This research illuminates evolutionary genetic mechanisms that contribute to diversification of secondary metabolite clusters in fungi.