Transcriptome of Pneumocystis jirovecii TSA
Transcriptome of Pneumocystis jirovecii during human Pneumocystis pneumonia
We collected a single broncho alveolar lavage from single human patient with Pneumocystis jirovecii pneumonia. Total RNA was extracted using the Ribopure Yeast Kit (Ambion), according to the manufacturerâ€™s instructions. To obtain sufficient amount of RNA, total RNA was amplified using NuGEN OvationRNA-seq system and used to build a non-strand-specific library. The library was then sequenced using Illumina HiSeq 2000 technologies
We collected a single broncho alveolar lavage from single human patient with Pneumocystis jirovecii pneumonia. Total RNA was extracted using the Ribopure Yeast Kit (Ambion), according to the manufacturerâ€™s instructions. To obtain sufficient amount of RNA, total RNA was amplified using NuGEN OvationRNA-seq system and used to build a non-strand-specific library. The library was then sequenced using Illumina HiSeq 2000 technologies