GSE173326
m6A-MeRIP sequencing of NK cells
Total RNAs were isolated from WT splenic NK cells, and subjected to standard m6A MeRIP, in two replicates, using Illumina Novaseq 6000 platform. The raw sequencing reads were mapped to the genome of Mus musculus (mm10) with default parameters. ExomePeak was used to identify m6A peaks, which were annotated by intersection with gene architecture using ChIPseeker. Sequence motifs enriched in peak regions were identified using Homer. Overall design: m6A modified mRNAs from WT splenic NK cells were immunopriciptated and enriched with m6A specific antibody, followed by deep sequencing, in two replicates, using Illumina Novaseq 6000 platform.