5fC_sequencing_of_Tdg_knockout_embryos-sc-3136
5fC_sequencing_of_Tdg_knockout_embryos
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ In this study we aim to assess the 5-formylcytosine profile in tissues from control and Thymine DNA Glycosylase (TDG)-deleted embryos by pulling down and sequencing 5fC-containing DNA (method from Raiber et al., 2012).
We will compare 5fC-pulldown libraries from tissues from Thymine DNA-glycosylase knockout and control embryos in order to characterise the effect of TDG deletion on the formylcytosine profile in the embryo.