ena-STUDY-IRIBHM-21-09-2016-17:55:23:737-109
Intratumour heterogeneity in a BRAV600E-mutated papillary thyroid carcinoma
A 51 year old female was diagnosed with a bilateral PTC (right lobe: 0,6 cm; left lobe: 2,8cm) with concurrent metastatic involvement of the lymph nodes. This corresponded to a stage IVA PTC (pT3N1b). She was treated with a total thyroidectomy and a lymphadenectomy, with 5 nodal metastases in the recurrent and mediastinal areas. Iodine-131 radiotherapy was administered one month after surgery. Twelve months after thyroidectomy, she was operated for nodal recurrences (4 nodal metastases from the left cervical, 3 of which were included in the study, annotated M2, M3 and M4). Histopathological examination revealed a classical PTC developing in a goiter context, and suggested a tumour cell fraction greater than 70%. Exome were sequenced for 4 blocks of the left-lobe tumour, 1 block of the right-lobe tumour, the 3 nodal metastases, 2 normal lymh nodes, 3 blocks of normal adjacent thyroid, and 1 blood sample.
A 51 year old female was diagnosed with a bilateral PTC (right lobe: 0,6 cm; left lobe: 2,8cm) with concurrent metastatic involvement of the lymph nodes. This corresponded to a stage IVA PTC (pT3N1b). She was treated with a total thyroidectomy and a lymphadenectomy, with 5 nodal metastases in the recurrent and mediastinal areas. Iodine-131 radiotherapy was administered one month after surgery. Twelve months after thyroidectomy, she was operated for nodal recurrences (4 nodal metastases from the left cervical, 3 of which were included in the study, annotated M2, M3 and M4). Histopathological examination revealed a classical PTC developing in a goiter context, and suggested a tumour cell fraction greater than 70%. Exome were sequenced for 4 blocks of the left-lobe tumour, 1 block of the right-lobe tumour, the 3 nodal metastases, 2 normal lymh nodes, 3 blocks of normal adjacent thyroid, and 1 blood sample.