22634603-3e5d-49ba-adfa-fcd3939750fd
Seed-vectored Endophytic Fungi
Endophytic fungi act as seed endosymbiont which play very crucial role in growth and development of seeds. Seed-vectored endophytic fungi establish an everlasting association with seeds which travels generation to generation. To explore composition and diversity of endophytic fungi in Alpinia zerumbet seeds, high throughput Illumina MiSeq sequencing technique was employed at following stages; fruit formation period (YSJ1), young fruit period (YSJ2), early mature period (YSJ3), middle mature period (YSJ4) and late mature period (YSJ5). In total 906694 sequence read and 745 operational taxonomic units (OTUs) were obtained which were further classified into 8 phyla, 30 classes, 73 orders, 163 families, 302 genera and 449 species. The highest endophytic fungal diversity was observed at YSJ5. Cladosporium, Kodamaea, Hannaella, Mycothermus, Gibberella, Sarocladium, Neopestalotiopsis were genera with highest abundance. FUNGuild analysis revealed that endophytic fungi were undefined saprotroph, plant pathogens, animal pathogen-endophyte-lichen parasite-plant pathogen-wood saprotroph and soil saprotrophs. Alternaria, Fusarium, Cladosporium and Sarocladium were also in abundance which are potential probiotics and can be used as biocontrol agent. This study is a part of Sustainable Development Goals of United Nations Organization (UNO), to “Establish Good Health and Well-Being.”
Endophytic fungi act as seed endosymbiont which play very crucial role in growth and development of seeds. Seed-vectored endophytic fungi establish an everlasting association with seeds which travels generation to generation. To explore composition and diversity of endophytic fungi in Alpinia zerumbet seeds, high throughput Illumina MiSeq sequencing technique was employed at following stages; fruit formation period (YSJ1), young fruit period (YSJ2), early mature period (YSJ3), middle mature period (YSJ4) and late mature period (YSJ5). In total 906694 sequence read and 745 operational taxonomic units (OTUs) were obtained which were further classified into 8 phyla, 30 classes, 73 orders, 163 families, 302 genera and 449 species. The highest endophytic fungal diversity was observed at YSJ5. Cladosporium, Kodamaea, Hannaella, Mycothermus, Gibberella, Sarocladium, Neopestalotiopsis were genera with highest abundance. FUNGuild analysis revealed that endophytic fungi were undefined saprotroph, plant pathogens, animal pathogen-endophyte-lichen parasite-plant pathogen-wood saprotroph and soil saprotrophs. Alternaria, Fusarium, Cladosporium and Sarocladium were also in abundance which are potential probiotics and can be used as biocontrol agent. This study is a part of Sustainable Development Goals of United Nations Organization (UNO), to “Establish Good Health and Well-Being.”