GSE210560
Effect of theta toxin secreted by live S. typhimurium inside spheroids of lung cancer cell lines H460 and H1819
To investigate the effect of bacterially secreted Theta toxin on lung cancer cells, we established co-culture of live S. typhimurium and spheroids of H460 and H1819 cell lines. Once the bacteria colonized inside the spheroids, we induced the expression of Theta toxin by the bacteria. We extracted RNA after 1 week of treatment. Overall design: We analyzed the transcriptional profiling from RNA-seq data comparing lung cancer spheroids with or without the expression of Theta toxin