GSE203392
Knock-outs of TUT7 and 3'hExo show that they cooperate in histone mRNA maintenance and degradation
Metazoan histone mRNAs are the only cellular eucaryotic mRNAs that are not polyadenylated, ending instead in a conserved stemloop. SLBP is bound to the 3' end of histone mRNA and is required for translation of histone mRNA. Their expression is tightly cell-cycle regulated. A major regulatory step is rapid degradation of histone mRNA at the end of S-phase or when DNA synthesis is inhibited in S-phase. 3'hExo, a 3' to 5' exonuclease, binds to the SLBP/SL complex, and trims histone mRNA to 3 nts after the stemloop.  Together with a terminal uridyl transferase, 3'hExo maintains the length of the histone mRNA during S-phase. 3'hExo is essential for initiating histone mRNA degradation on polyribosomes, initiating degradation into the 3' side of the stemloop.  There is extensive uridylation of degradation intermediates in the 3' side of the stem when histone mRNA is degraded.  Here we knocked out TUT7 and 3'hExo and we show that both modification of histone mRNA during S-phase and degradation of histone mRNA involve the interaction of 3'hExo, and a specific TUTase, TENT3B (TUT7, CHHC6).  Knockout of 3'hExo prevents initiation of 3' to 5' degradation, stabilizing histone mRNA, while knockout of TUT7 prevents uridylation of the mRNA degradation intermediates slowing the rate of degradation.  In synchronized 3'hExo KO cells, histone mRNA degradation is delayed but the histone mRNA is degraded prior to mitosis by a different pathway. Overall design: CRISPR Cas9 Knock out and Rescue of TUT7 and 3'hExo in HCT116 Cells, with histone specific RNA sequencing to study the 3' termini via EnD-Seq