GSE183700
The Role of DNMT1 and C/EBPa in the Regulation of CYP11A1 Expression During Syncytialization of Human Placental Trophoblasts
Progesterone synthesized in the placenta is essential for pregnancy maintenance. CYP11A1 is a key enzyme in progesterone synthesis, and its expression increases greatly during trophoblast syncytialization. However, the underlying mechanism remains elusive. Here, we demonstrated that passive demethylation of CYP11A1 promoter accounted for the upregulation of CYP11A1 expression during syncytialization with the participation of the transcription factor C/EBPa. We found that the methylation rate of a CpG locus in the CYP11A1 promoter was significantly reduced along with decreased DNA methyltransferase 1 (DNMT1) expression and its enrichment at the CYP11A1 promoter during syncytialization. DNMT1 overexpression not only increased the methylation of this CpG locus in the CYP11A1 promoter, but also decreased CYP11A1 expression and progesterone production. In silico analysis disclosed multiple C/EBPa binding sites in both CYP11A1 and DNMT1 promoters. C/EBPa expression and its enrichments at both the DNMT1 and CYP11A1 promoters were significantly increased during syncytialization. Knocking-down C/EBPa expression increased DNMT1 while it decreased CYP11A1 expression during syncytialization. Conclusively, C/EBPa plays a dual role in the regulation of CYP11A1 during syncytialization. C/EBPa not only drives CYP11A1 expression directly, but also indirectly through downregulation of DNMT1, which leads to decreased methylation in the CpG locus of the CYP11A1 promoter, resulting in increased progesterone production during syncytialization. Overall design: RNA sequecing was used  to indetify the change of genes durning placental trophoblasts syncytialization