GSE172110
Diversity of developing peripheral glia revealed by single cell RNA sequencing
We collected dorsal root ganglion (DRG), sciatic nerve (SN) and cochlea tissues from each E14, E18 and P14 plp-GFP+ mice. This allowed for a comparison of plp-GFP+ glia from lumbar (DRG+SN) and cochlea tissues within the same mice. The transcriptional composition of all samples was assessed using single-cell RNA-seq (scRNA-seq). Overall design: Lumbar tissues (DRG+SN) and cochlea were enzymatically dissociated, GFP+ glia were FACS sorted and profiled by Indrop Single cell v3 method.