PRJNA824957
Mus musculus Raw sequence reads
To understand the mechanism of APCs deficiencies in tumor bearing mice, we examined the transcriptomic changes in APCs by RNA sequencing of cells isolated from tumor free and B16F10 tumor bearing mice after tumor vaccine immunization. we also examined the functional reprogramming of GMPs induced by the presence of subcutaneous tumors. In light of the gene transcription deficiencies of APCs in response to tumor vaccines in the tumor bearing mice, we focused on the overall accessibility of chromatin in GMPs by performing global epigenetic profiling of open chromatin using an assay for transposase accessible chromatin using high throughput sequencing (ATAC sequencing).