GSE39926
Evolutionary potential, cross-stress behavior and the genetic basis of acquired stress resistance in Escherichia coli
We evolved Escherichia coli cells over 500 generations under five environments that include four abiotic stressors: osmotic, acidic, oxidative, n-butanol, and control The goal of the experiment: Bacterial populations have a remarkable capacity to cope with extreme environmental fluctuations in their natural environments. In certain cases, adaptation to one stressful environment provides a fitness advantage when cells are exposed to a second stressor, a phenomenon that has been coined as cross-stress protection. A tantalizing question in bacterial physiology is how the cross-stress behavior emerges during adaptation and what the genetic basis of acquired stress resistance is. Overall design: RNA profiles were obtained for six E. coli strains evolved for 500 generations under abiotic stressors; two technical replicates for each strain where sequenced by Illumina GAII analyzer