Copy_number_variation_in_mouse_oesophagus__p53-sc-5200
Copy_number_variation_in_mouse_oesophagus__p53
We wish to determine how the mutational burden in mouse oesophagus changes in transgenic mice induced to carry mutations known to cover significant areas of human oesophagus.  Oesophaguses from mice carrying either mutations in P53, Notch1 or with altered immune systems  will be collected and DNA extracted from 2mm2 biopsies.   Low depth WGS will allow us to look at changes in copy number and structureal rearragements.  In some cases the mice will be further treated with DEN a carcinogen present in cigarette smoke.
We wish to determine how the mutational burden in mouse oesophagus changes in transgenic mice induced to carry mutations known to cover significant areas of human oesophagus.  In some cases the mice will be further treated with DEN a carcinogen present in cigarette smoke.  Ultilising a combination of targetted high-depth sequencing and low depth WGS we will attempt to understand how the mutational landscape of the oesophagus changes with different background mutations.