Nicotiana tabacum L. cv. SR1 stem mRNA 5 developmental stages
Genome-wide transcriptome analysis of the transition from primary to secondary stem development in tobacco using RNA-seq
To obtain an overview of the tobacco stem development transcriptome, a cDNA library was generated from an equal mixture of RNA isolated from 5 wild type tobacco tissue samples and 3 35S::PtSEP3 (due to the data needed for another experiment, not described here) tobacco tissue samples, and pair end sequenced using the Illumina platform.
Tobacco plants have been used as a model system for the testing of new procedures and in research on the function of specific genes. They have the advantage of facile transformation of selected genotypes, short generation times. Yet little is understood about its genes involved in secondary growth, so next generation sequencing methods could solve the problem.