DRP000011
Comprehensive analysis of cytoplasmic mRNAs in HT29 cell.
We analyzed the mRNA profile in cytoplasm and in polysome during the            endoplasmic reticulum (ER) stress using second generation sequencers. We compared three            time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ??g/mL ER            stress inducible agent Tunicamycin (Tm)). This is the first time mRNA in ???polysome??? is            analyzed using the next generation sequencers and compared with that of cytoplasm.
Expression profile in HT29 cell exposed to ER stress was attempted. We used            shotgun sequencing method, in which next gene sequencing technology and polysome            analysis were combined.