PRJNA261434
Escherichia coli str. K-12 substr. MG1655 Targeted Locus (Loci)
We systematically mapped the sequence space of the four key specificity residues in the Escherichia coli histidine kinase PhoQ. The cells were selected using FACS with a transcriptional reporter and magnesium starvation. The residues were amplified using PCR and sequenced using Illumina HiSeq.