GSE210052
DNA barcoding reveals ongoing immunoediting of clonal cancer populations during metastatic progression and in response to immunotherapy [DNA barcodes: WT_nude_and_NSG_4T1_BC5000]
To investigate immunoediting at the primary tumour, we used DNA barcoding combined with NGS. By stably integrating 4T1 murine cancer cell line with 5000 unique DNA barcodes (1 barcode per cell), we can trace how barcode (and therefore subclonal) diversity changes over time and in reponse to the endogenous immune system in immunocompetent mice compared to immunocompromised mice. Overall design: The data provided are generated by targete barcode sequencing of primary tumours and matching lungs generated in mice from barcoded 4T1 cells. Samples are generated from immunocompetent BALB/c mice or immunocompromised NSG mce, with both primary tumour and matching lung tissue analysed.