GSE128390
Histone Modifications and Chromatin Accessibility in Endothelial Cells under Shear Stress (ATAC-seq)
This data set reveals the changes of histone modifications and chromatin accessibility in human umbilical vein endothelial cells (HUVECs) under atheroprotective pulsatile shear (PS), atheroprone oscillatory shear (OS), or with KLF4 overexpression. Using ChIP-Seq, we defined the H3K27ac and H3K4me1 enrichment under PS and OS conditions. Using ATAC-seq, we identified the chromatin accessibility under KLF4 overexpression. Overall design: ECs were infected with adenovirus-KLF4 or the empty vehicle (Ad-null) for 24 hr. Chromosome accessibility were evaluated with ATAC-seq. Two biological replicates were collected for each condition.