GSE131253
Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Murine Fibroblast Fibroproliferative Response with TGFß
AKR-2B cells were grown in an anchorage independent system to assess transcriptional profile of the TGFß-dependent fibroproliferative response. Overall design: AKR-2B cells were grown in soft agar -/+TGFß, and colonies isolated and total RNA purified at 2, 4, and 8 days, then sent for NGS of mRNA and mRNA, and analyzed for the differentially increased genes associated with TGFß stimulation.