E-MTAB-7998
Bindingof ZC3H20 and ZC3H21 to RNA
Procyclic-form trypanosomes (Liser 427) constitutively expressing either TAP-ZC3H20 or TAP-ZC3H21, with no other copy of the relevant gene, were used. The protein was pulled down with an IgG column , then the protein and bound RNA was eluted using TEV protease. RNA was sequenced from unbound (flow-through) and bound (eluate) fractions.
Procyclic-form trypanosomes (Liser 427) constitutively expressing either TAP-ZC3H20 or TAP-ZC3H21, with no other copy of the relevant gene, were used. The protein was pulled down with an IgG column , then the protein and bound RNA was eluted using TEV protease. RNA was sequenced from unbound (flow-through) and bound (eluate) fractions.