PRJNA577124
AAVHSC mediated gene integration at HBB locus
We tested if AAVHSC can edit HBB locus to therapeutic level. HBB locus was PCR amplified, made into Illumina sequencing library using Nextera kit, and sequenced with MiSeq v3 PE 150. Fastq files were used for counting reads with edited sequence and WT (unedited) sequence.