Histone_marks_controlling_IAP_retrotransposons_activity-sc-3460
Histone_marks_controlling_IAP_retrotransposons_activity
We will associate H3K9me3 enrichment  with IAP methylation and transcritpion changes in ES cells. IAPs are normally kept methylated in ES cells  and only in Dnmt1 knock out ES cells this class of transposons is being demethylated (Arand et al., PLoS Genet, 8(6), June 2012). The same demethylation is happening in 2i conditions (Seisenberger et al., Molecular Cell, 48(6):849{862, December 2012). Dnmt1 conditional knockdown ES cells will be used for studying  H3K9me3 enrichment at different time points of Dnmt1 knockdown (Liu et al., Nat Commun, 4:1563, March 2013).  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We will associate H3K9me3 enrichment  with IAP methylation and transcritpion changes in ES cells. IAPs are normally kept methylated in ES cells  and only in Dnmt1 knock out ES cells this class of transposons is being demethylated (Arand et al., PLoS Genet, 8(6), June 2012). The same demethylation is happening in 2i conditions (Seisenberger et al., Molecular Cell, 48(6):849{862, December 2012). Dnmt1 conditional knockdown ES cells will be used for studying  H3K9me3 enrichment at different time points of Dnmt1 knockdown (Liu et al., Nat Commun, 4:1563, March 2013).