GSE124570
Genome-wide transcriptome profiling of Acidithiobacillus ferrooxidans ATCC 23270 in response to the cyc2 knockout
The outer-membrane c-type cytochrome Cyc2 is generally considered to be the initial electron acceptor in iron respiratory chain of Acidithiobacillus ferrooxidans ATCC 23270, a model microorganism in acidophilic bioleaching environment. In our work, however, the knockout of cyc2 did not result in impaired Fe(II) consumption or growth capacity. To screen the potential genes for alternative initial electron acceptors other than Cyc2, RNA-Seq was employed to compare global gene expressions in the A. ferrooxidans ATCC 23270 wild type and the ?cyc2 mutant grown on Fe(II) or switched energy source from S0 to Fe(II). The data focused on 29 up-regulated and 19 down-regulated genes in the mutant under both conditions, among which AFE_1428 was the most highest one. in-silico analysis also suggested that the product of AFE_1428 might act as an alternative initial electron acceptor when Cyc2 was absent, which needs to be further validated. Overall design: Differential mRNA profiles of A. ferrooxidans ?cyc2 mutant were generated by Next-generation sequencing using wild-type strain grown on Fe(II) or switched energy source from S0 to Fe(II) as  control.