GSE114495
Effect of miR17-92 and miR-106b-25 loss in primary calvarial cells
Gene expression profiling of  primary calvarial cells with miR-17-92:miR-106b-25 conditional deletion Overall design: 10-day-old primary calvarial cells of floxed miR-17-92(fl/fl):miR-106b-25(-/-) mice were isolated with collagenase digestion, cultured in alpha-MEM containing 10% fetal bovine serum, and  infected with adenoviruses  expressing Cre or YFP.  3 days after adenovirus infection, RNA was extracted with Trizol, purified with Zymo spin columns, and subjected to RNA-seq