PRJNA435480
Cajanus cajan isolate:11A_rep1 | cultivar:AKCM11 Raw sequence reads
Total RNA libraries were prepared using isolated total RNA according to the protocol ofIllumina TrueSeq RNA Library Preparation Kit (https://support.illumina.com/). The protocolincludes purification and fragmentation of mRNA, first stand cDNA synthesis followed bysecond stand cDNA synthesis, end-repairing of the cDNA, 3 end adenylation, followed byadapter ligation and PCR amplification. The libraries were checked for the average mean size(260 bp) on Agilent DNA 1000 chip using Agilent 2100 Bioanalyzer. The libraryconcentration was checked using Qubit Fluorometer. After obtaining the Qubit concentrationfor each of the respective libraries, cluster generation was performed using Illumina CBotinstrument and finally loaded onto Illumina HiSeq 1000 platform for paired-end sequencing.