E-MTAB-5732
Gain of chromatin loops marks the exit from naive pluripotency - ChIP seq for CTCF and Rad21
ChIP-seq to map the binding sites for CTCF and cohesin subunit Rad21  in the naive mES cells (46C cell line grown in the 2i/LIF condition)  and in the neural stem cells (derived from the 46C ES cells using  the mono-layer differentiation protocol, grown in the N2B27 medium  these cells are Nestin+). The naive mES cells were grown in two different media  (fetal bovine serum, FBS and 2i/LIF culture - naive pluripotency conditions) as detailed in the growth protocols.
ChIP-seq to map the binding sites for CTCF and cohesin subunit Rad21  in the naive mES cells (46C cell line grown in the 2i/LIF condition)  and in the neural stem cells (derived from the 46C ES cells using  the mono-layer differentiation protocol, grown in the N2B27 medium  these cells are Nestin+). The naive mES cells were grown in two different media  (fetal bovine serum, FBS and 2i/LIF culture - naive pluripotency conditions) as detailed in the growth protocols.