GSE185004
Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS]
We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level. Overall design: RRBS-seq of ciTotiSC with two replicates.