GSE130904
H3K36 methyltransferase SETD2 regulates lymphoid and neural development [seq]
We study the role of loss of Setd2 and H3K36me3 in early B cell development through NGS methods including RNA, ChIP and ATAC Seq of sorted B cell populations of control and Setd2KO primary B cells from mice. Overall design: From age-matched littermate controls and Setd2KO mouse, we sorted early proB cells (B220+CD43+CD19-Cd25-IgM-) and performed ATAC-seq, RNA-Seq, and ChIP-seq using an antibody against H3K36me3.