ena-STUDY-NORTHUMBRIA UNIVERSITY, NEWCASTLE-29-07-2019-09:18:12:843-1041
Changes in the Gut Microbiota of Mice Orally Exposed to Methylimidazolium Ionic Liquids: an Observational Study
Ionic liquids are salts used in a variety of industrial processes, and being relatively non-volatile, are proposed as environmentally-friendly replacements for existing volatile liquids. Methylimidazolium ionic liquids resist complete degradation in the environment, likely because the imidazolium moiety does not exist naturally in biological systems.  However, there is limited data available regarding their mammalian effects in vivo.This study aimed to examine the effects of exposing mice separately to 2 different methylimidazolium ionic liquids (BMI and M8OI) through their addition to drinking water.  Potential effects on key target organs for acute toxicity – the liver and kidney – and the gut microbiome were examined.Adult male mice were exposed to drinking water containing ionic liquids at a concentration of 440 mg/L for 18 weeks prior to examination of tissues, serum, urine and gut microbiome.  Histopathology was performed on tissues and clinical chemistry on serum for biomarkers of hepatic and renal injury.  Bacterial DNA was isolated from the gut contents and subjected to 16S rRNA sequencing.Mild hepatic and renal effects were limited to glycogen depletion and mild degenerative changes respectively.  No hepatic or renal adverse effects were observed.  In contrast, Ionic liquid exposure altered gut microbial composition but not overall alpha diversity. Proportional abundance of Lachnospiraceae, Clostridia and Coriobacteriaceae was significantly enriched in ionic liquid-exposed mice, as were predicted KEGG functional pathways associated with xenobiotic and amino acid metabolism.Exposure to ionic liquids via drinking water results in marked changes in the gut microbiome in mice prior to any overt pathological effects in target organs.  Ionic liquids may be an emerging risk to health through their potential effects on the gut microbiome, which is implicated in the causes and/or severity of an array of chronic disease in man.
Ionic liquids are salts used in a variety of industrial processes, and being relatively non-volatile, are proposed as environmentally-friendly replacements for existing volatile liquids. Methylimidazolium ionic liquids resist complete degradation in the environment, likely because the imidazolium moiety does not exist naturally in biological systems.  However, there is limited data available regarding their mammalian effects in vivo.This study aimed to examine the effects of exposing mice separately to 2 different methylimidazolium ionic liquids (BMI and M8OI) through their addition to drinking water.  Potential effects on key target organs for acute toxicity – the liver and kidney – and the gut microbiome were examined.Adult male mice were exposed to drinking water containing ionic liquids at a concentration of 440 mg/L for 18 weeks prior to examination of tissues, serum, urine and gut microbiome.  Histopathology was performed on tissues and clinical chemistry on serum for biomarkers of hepatic and renal injury.  Bacterial DNA was isolated from the gut contents and subjected to 16S rRNA sequencing.Mild hepatic and renal effects were limited to glycogen depletion and mild degenerative changes respectively.  No hepatic or renal adverse effects were observed.  In contrast, Ionic liquid exposure altered gut microbial composition but not overall alpha diversity. Proportional abundance of Lachnospiraceae, Clostridia and Coriobacteriaceae was significantly enriched in ionic liquid-exposed mice, as were predicted KEGG functional pathways associated with xenobiotic and amino acid metabolism.Exposure to ionic liquids via drinking water results in marked changes in the gut microbiome in mice prior to any overt pathological effects in target organs.  Ionic liquids may be an emerging risk to health through their potential effects on the gut microbiome, which is implicated in the causes and/or severity of an array of chronic disease in man.