DRP008753
Sequencing of EcoSeq libraries
The Enzymatically Cleaved and Optimal Sequencing (EcoSeq) method was able to count somatic mutations in a single DNA molecule with high efficiency (low sequencing cost), high accuracy (distinction of single-stranded premutagenic lesions and sequence errors), and high sensitivity (detection limit of 3Ã?10^-8 bp). EcoSeq has the potential to reveal accumulation of somatic mutations and exposure to environmental factors in any DNA samples, and will contribute to cancer risk estimation.