PRJNA766590
Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis
Here we present metagenomic criteria for microorganism detection and a metagenomic method able to be performed with sub-nanogram amounts of starting material, which controls for exogenous contamination to circumvent these and other pitfalls. We use this method to show that human mid-gestational amniotic fluid has no detectable virome or microbiome, supporting the sterile womb hypothesis.