GSE96596
RNA-Sequencing Analysis of the Effects of UVA Radiation on Keratinocytes
Normal human epidermal keratinocytes were treated with sham or UVA (20J/cm2) radiation in duplicate. 6h after irradiation, samples were purified using Qiagen Rneasy kit, RNA quality assessment was performed using Agilent Bioanalyzer, and library preparation was performed using oligodT selection. RNA-Seq was performed on Illumina HiSeq2000 platform with 50 bp single-end reads. Analysis used the Tuxedo pipeline. Results were validated by qRT-PCR. Overall design: Transcriptome of sham and UVA-irradiated normal human epidermal keratinocytes