Transcriptome_profiling_of_zebrafish_tcf7l1a_m881__mutant_embryos-sc-2943
Transcriptome_profiling_of_zebrafish_tcf7l1a_m881__mutant_embryos
Adult sibling homozygous wild type and tcf7l1a mutants were paired and Total RNA was trizol extracted from wild type and maternal zygotic homozygous tcf7l1a mutant embryos at 90% epiboly stage (9 hours post fertilization). ZFIN Identifier ZDB-GENE-980605-30, PMID:11057671. The 3’ end of fragmented RNA was pulled down using polyToligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using Illumina HiSeq paired-end sequencing. Protocol: Total RNA was extracted from zebrafish embryos using Trizol and DNase treated. Chemically fragmented RNA was enriched for the 3’ ends by pulled down using an anchored polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
3’ capture sequence data prepared from RNA of tcf7l1a homozygous mutant and wild type zebrafish embryos