ena-STUDY-INSTITUT PASTEUR-19-10-2016-12:19:52:371-545
Habitat-related differences in larval exposure to bacteria drive adult trait variation in a mosquito vector.
Biotic and abiotic conditions experienced during larval development of holometabolous insects can impact adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. We addressed this question in the mosquito Aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in Sub-Saharan Africa. Targeted metagenomics revealed contrasted bacterial communities in the water of natural larval development sites in Gabon. Experimental exposure to different native bacterial isolates during larval development resulted in significant differences in pupation rate and adult body size, but not lifespan. Larval exposure to an Arthrobacter (Asp_ivi) isolate resulted in larger bacterial loads in adult midguts, decreased antibacterial activity in adult hemolymph, and reduced dengue-1 virus dissemination titer. Together, these data provide the proof of concept that larval exposure to different bacteria can drive variation in adult traits underlying vectorial capacity.
Biotic and abiotic conditions experienced during larval development of holometabolous insects can impact adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. We addressed this question in the mosquito Aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in Sub-Saharan Africa. Targeted metagenomics revealed contrasted bacterial communities in the water of natural larval development sites in Gabon. Experimental exposure to different native bacterial isolates during larval development resulted in significant differences in pupation rate and adult body size, but not lifespan. Larval exposure to an Arthrobacter (Asp_ivi) isolate resulted in larger bacterial loads in adult midguts, decreased antibacterial activity in adult hemolymph, and reduced dengue-1 virus dissemination titer. Together, these data provide the proof of concept that larval exposure to different bacteria can drive variation in adult traits underlying vectorial capacity.