GSE130536
High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [human K562 and muring 3T3]
While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages. Overall design: A mixture of human K562 and murine 3T3 cells was profiled in various quantities to access cross-talk between cells in our combinatorial setting using 48-plex barcoded Tn5