ena-STUDY-GARVAN INSTITUTE OF MEDICAL RESEARCH-12-07-2019-04:06:44:975-593
Direct RNA sequencing data for human cell line k562 with ~10% sequins, a spike-in reference standard.
Generated on 22 November 2017 using 500ng total RNA extracted from k562 cells and 50ng of RNA sequins Mix A (final concentration after sample preparation = 1.51 ng/uL).Nanopore MinION flowcell FAH35825 (R9.4 chemistry), displayed 1323 active channels at commencement of run.Sequencing protocol RNA001.Base called with Albacore version 2.1.3.Includes albacore summary statistics (bundled in .tgz) and a generic quality report of the run (.pdf).Sequin reference sequences and expected abundances for Mix A can be found at http://www.sequin.xyz/downloads/  =====Update (7 July 2019): Added fastq and sequencing summary resulting from updated base calling using guppy high-accuracy (k562_guppyHAC.tgz, guppy v3.1.5 using R9.4.1 70bps RNA pore model).  Reference: https://doi.org/10.6084/m9.figshare.5797287.v3
Generated on 22 November 2017 using 500ng total RNA extracted from k562 cells and 50ng of RNA sequins Mix A (final concentration after sample preparation = 1.51 ng/uL).Nanopore MinION flowcell FAH35825 (R9.4 chemistry), displayed 1323 active channels at commencement of run.Sequencing protocol RNA001.Base called with Albacore version 2.1.3.Includes albacore summary statistics (bundled in .tgz) and a generic quality report of the run (.pdf).Sequin reference sequences and expected abundances for Mix A can be found at http://www.sequin.xyz/downloads/  =====Update (7 July 2019): Added fastq and sequencing summary resulting from updated base calling using guppy high-accuracy (k562_guppyHAC.tgz, guppy v3.1.5 using R9.4.1 70bps RNA pore model).  Reference: https://doi.org/10.6084/m9.figshare.5797287.v3