GSE128389
Histone Modifications and Chromatin Accessibility in Endothelial Cells under Shear Stress (ChIP-seq)
This data set reveals the changes of histone modifications and chromatin accessibility in human umbilical vein endothelial cells (HUVECs) under atheroprotective pulsatile shear (PS), atheroprone oscillatory shear (OS), or with KLF4 overexpression. Using ChIP-Seq, we defined the H3K27ac and H3K4me1 enrichment under PS and OS conditions. Using ATAC-seq, we identified the chromatin accessibility under KLF4 overexpression. Overall design: PS or OS was applied to endothelial cells (ECs) with a shear stress of 12 ± 5 dyn/cm2 or 1 ± 4 dyn/cm2 for 16 hr. The histone marks were assayed by ChIP-seq.