Raindance Experiment
Targeted sequencing of the human X chromosome exome
We used a RainDance Technologies (RDT) expanded content library to enrich the human X chromosome exome (2.5Mb) from 26 male samples followed by Illumina sequencing. Our multiplex primer library covered 98.05% of  the human X chromosome exome in a single tube with 11,845 different PCR amplicons. Illumina sequencing of 24  male samples showed coverage for 97% of the targeted sequences. Sequence from 2 HapMap samples confirmed  missing data rates of 2–3% at sites successfully typed by the HapMap project,with an accuracy of at least ~99.5% as compared to reported HapMap genotypes. Our demonstration that a RDT expanded content library can efficiently enrich and enable the routine sequencing of the human X chromosome exome suggests a wide variety of potential research and clinical applications for this platform.