GSE113585
Profiling of colorectal tumor-infiltrating CD8 T cells according to their expression status of CD39
Human tumors are infiltrated by various immune cells, including CD8 T cells. CD8 T cells express unique receptors that can recognize peptides at the host's cells, including tumor cells. After probing the antigen specificity of ex-vivo tumor-infiltrating CD8 T cells from human tumors, we hypothesized that expression of CD39 was correlated with tumor-specificity. The present experiment aims at better characterizing ex-vivo CD39+ vs CD39- CD8 T cells. Overall design: CD39- and CD39+ CD8 T cells were FACS sorted from 8 fresh tumor samples and their RNA extracted for transcriptomic profiling. Additionally, Effector memory and Naive CD8+ T cells were FACS sorted from 5 healthy donors' peripheral blood.