ena-STUDY-MAINZ-07-04-2017-12:29:52:633-31
Transcriptome data of cultured neuroglobin-floxed and neuroglobin-knockout neural stem cells from mouse were used to investigate the molecular phenotype of the neuroglobin-knockout status by differential gene expression analysis.
In the quest to unravel the functional significance of neuroglobin (Ngb) the present study aimed at investigating the role of Ngb in the growth and proliferation of  neural stem cells by comparing an Ngb-floxed (Ngbfl-)NSC line, equivalent to the wild type cellular situation, with an in-house created Ngb knockout (NgbKO-)NSC line. NgbKO-NSCs were characterised by an increased growth and proliferation capacity in vitro, supported by RNA sequencing results reporting the downregulation of Cdkn1a and the upregulation of Cdk6, both enhancing the cell cycle. Based on additional gene ontology enrichment and pathway analyses, we postulate that in a wild type cellular situation Ngb may exert a repressive function on the cell cycle in an AKT-MDM2-TP53 dependent manner.
In the quest to unravel the functional significance of neuroglobin (Ngb) the present study aimed at investigating the role of Ngb in the growth and proliferation of  neural stem cells by comparing an Ngb-floxed (Ngbfl-)NSC line, equivalent to the wild type cellular situation, with an in-house created Ngb knockout (NgbKO-)NSC line. NgbKO-NSCs were characterised by an increased growth and proliferation capacity in vitro, supported by RNA sequencing results reporting the downregulation of Cdkn1a and the upregulation of Cdk6, both enhancing the cell cycle. Based on additional gene ontology enrichment and pathway analyses, we postulate that in a wild type cellular situation Ngb may exert a repressive function on the cell cycle in an AKT-MDM2-TP53 dependent manner.