CRISPR_Cas9_off_targets-sc-6107
CRISPR_Cas9_off_targets
Here, we are using  whole genome sequencing to study possible off-targets after CRISPR-Cas9 induction in Mycobacterium abscessus This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We have analysed possible off targets of CRISPR-CAs9 in Mycobacterium abscessus by picking several colonies after CRISPR activation and appliying whole genome sequencing to study the nature of induced mutations.