E-MTAB-10877
RNA-Seq data of Trpm7+/+ and Trpm7-/- embryonic stem cell clones
Partial exon deletion in Trpm7 gene was confirmed by lack of transcripts according to the RNA sequencing in two stem cell clones. For this mice were genetically modified by partial deletion of exon 2 and embryonic stem (ES) cell lines were generated. Isolated RNA of two ES clones was sequenced with Illumina NextSeq 500 Sequencer, which generated 75 bases long 'single-end' reads.
Partial exon deletion in Trpm7 gene was confirmed by lack of transcripts according to the RNA sequencing in two stem cell clones. For this mice were genetically modified by partial deletion of exon 2 and embryonic stem (ES) cell lines were generated. Isolated RNA of two ES clones was sequenced with Illumina NextSeq 500 Sequencer, which generated 75 bases long 'single-end' reads.