GSE52534
Role of 3'' UTR in spreading of siRNAs
We use small RNA sequencing to look for spreading of secondary and tertiary siRNAs along transcripts targeted by ectopic hairpin-derived primary siRNAs. We find that signals within the 3'' UTR inhibit siRNA spreading. Overall design: sequencing of 20-30nt sRNAs from Argonaute immunoprecipitation