DRP005235
Elucidation of chromatin interactions for hormone therapy- resistance of ER positive breast cancer
The project aims to elucidate chromatin interactions between ESR1 promoter and other genomic regions in hormone therapy- resistant estrogen receptor (ER) positive breast cancer. We compared 4C-seq (chromosome conformation capture combined with high-throughput sequencing) data of human ER positive breast cancer cell line MCF7 and LTED (MCF7 cells after long term estrogen deprivation) cells. The nuclei from each cell type were fixed, digested by first restriction enzyme and ligated by DNA ligase. The purified DNA was digested by second restriction enzyme, ligated and libraries were prepared. ESR1 promoter was set as a bait for the 4C-seq.