ena-STUDY-Indian Statistical Institute-25-07-2019-09:34:35:281-932
Cancer Gene Panel Sequencing of PDAC and PAC
Here We have performed sequencing of 412 cancer gene panel in 5 PDAC ad 3 PAC tumour blood paired sample. We used Hiseq2500 platform to generate 100 bp pair end reads. We have identified total 48 somatic mutations from 8 patients in exons from 412 genes . These 48 somatic mutations comprise 65% (n=31) missense, 10% (n=5) synonymous, 10% (n=5) UTR, 8% (n=4) nonsense, 4% (n=2) InDel, and 2% (n=1) splice site mutations. Genes which were mutated in two or more than 2 samples are following: KMT2C (n=5), TP53 (n=3), SMAD4 (n=2), CTNNB1(n=2), ERBB2 (n=2), NOTCH1 (n=2), PARP1 (n=2), PIK3R1 (n=2), and PIK3CD (n=2) genes
Here We have performed sequencing of 412 cancer gene panel in 5 PDAC ad 3 PAC tumour blood paired sample. We used Hiseq2500 platform to generate 100 bp pair end reads. We have identified total 48 somatic mutations from 8 patients in exons from 412 genes . These 48 somatic mutations comprise 65% (n=31) missense, 10% (n=5) synonymous, 10% (n=5) UTR, 8% (n=4) nonsense, 4% (n=2) InDel, and 2% (n=1) splice site mutations. Genes which were mutated in two or more than 2 samples are following: KMT2C (n=5), TP53 (n=3), SMAD4 (n=2), CTNNB1(n=2), ERBB2 (n=2), NOTCH1 (n=2), PARP1 (n=2), PIK3R1 (n=2), and PIK3CD (n=2) genes