GSE122587
Insights into the mechanism underlying the therapeutic effects of raspberry ketone on human colorectal carcinoma cells revealed by RNA sequencing and bioinformatics analysis
Colorectal cancer (CRC) is the most commonly diagnosed cancer of the digestive system. Bioactive substances from plants are of particular research interest, due to their chemopreventive or chemotherapeutic properties against tumors. Raspberry ketone (RK), one of the major aromatic compounds in red raspberries, is widely used as a flavoring agent in foodstuffs, and for nutritional and medicinal purposes. In the present study, we found that RK inhibited the growth of DLD-1 CRC cells and identified differentially expressed genes (DEGs) by RNA sequencing in DLD-1 cells treated with RK. Transcriptome analyses indicated significant changes in the mRNA levels of 901 genes according to P - values < 0.01 (609 up- and 292 down-regulated) after treatment of DLD-1 cells with 400 µg/mL RK for 72 h. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that various cellular functions were involved in RK treatment. Interaction network analysis of GO terms and KEGG pathways revealed details of their interactions and predicted the key RK target pathways. These results provide evidence applicable to the prevention and therapy of CRC. Overall design: Identified differentially expressed genes (DEGs) by RNA sequencing in DLD-1 cells treated with RK