PRJNA551880
Soybean Amplicon Study Raw sequence reads
In 2017, we carried out amplicon sequencing on the Illumina Miseq platform to catalogue the foliar bacteria and fungi infecting soybean throughout its growing region in Manitoba, Canada. Fungi were identified using the Internal Transcribed Spacer (ITS) , which possesses multiple hypervariable regions that can provide high taxonomic resolution (Schoch et al. 2012). Bacteria were detected using the 16S ribosomal RNA (rRNA) gene, which is a region with similar characteristics to ITS in fungi (Armougom and Raoult 2009).