ena-STUDY-Leibniz Institute for Farm Animal Biology (FBN)-20-08-2019-08:34:22:424-75
Sequencing raw reads for pikeperch genome including Illumina paired-end and mate-pairs reads, and PaCbio Sequel subreads.
The pikeperch (Sander lucioperca) is a fresh and brackish water Percid fish natively inhabiting the northern hemisphere. This species is emerging as a promising candidate for intensive aquaculture production in Europe. Here, we report the raw sequencing data generated in the scope of pikeperch genome sequencing and assembly project. DNA sequencing raw data were generated with Illumina platform and PacBio Sequel System, respectively. The PacBio data were assembled into a final assembly size of  ~900 Mb covering 89% of the 1,014 Mb estimated genome size. The draft genome consisted of 1,966 contigs ordered into 1,313 scaffolds. The contig and scaffold N50 lengths are 3.0 Mb  and 4.9 Mb, respectively. The identified repetitive structures accounted for 39% of the genome. We utilized homologies to other ray-finned fishes, and ab initio gene prediction methods to predict 21,249 protein-coding genes in the S. lucioperca genome, of which 88% were functionally annotated by either sequence homology or protein domains and signatures search. The assembled genome spans 97.6% and 96.3% of Vertebrate respectively Actinopterygii single-copy orthologs. The outstanding mapping rate (99.9%) of genomic PE-reads on the assembly suggests an accurate and nearly complete genome reconstruction. This draft genome sequence is the first genomic resource for this promising aquaculture species. It will provide an impetus for genomic-based breeding studies targeting phenotypic and performance traits of captive pikeperch.
The pikeperch (Sander lucioperca) is a fresh and brackish water Percid fish natively inhabiting the northern hemisphere. This species is emerging as a promising candidate for intensive aquaculture production in Europe. Here, we report the raw sequencing data generated in the scope of pikeperch genome sequencing and assembly project. DNA sequencing raw data were generated with Illumina platform and PacBio Sequel System, respectively. The PacBio data were assembled into a final assembly size of  ~900 Mb covering 89% of the 1,014 Mb estimated genome size. The draft genome consisted of 1,966 contigs ordered into 1,313 scaffolds. The contig and scaffold N50 lengths are 3.0 Mb  and 4.9 Mb, respectively. The identified repetitive structures accounted for 39% of the genome. We utilized homologies to other ray-finned fishes, and ab initio gene prediction methods to predict 21,249 protein-coding genes in the S. lucioperca genome, of which 88% were functionally annotated by either sequence homology or protein domains and signatures search. The assembled genome spans 97.6% and 96.3% of Vertebrate respectively Actinopterygii single-copy orthologs. The outstanding mapping rate (99.9%) of genomic PE-reads on the assembly suggests an accurate and nearly complete genome reconstruction. This draft genome sequence is the first genomic resource for this promising aquaculture species. It will provide an impetus for genomic-based breeding studies targeting phenotypic and performance traits of captive pikeperch.