GSE107329
E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [5'OH-seq]
The purpose of this experiment was to characterize MazF cleavage specificity with single-nucleotide resolution by enriching and sequencing 5'-OH ends (a 5'-OH end is generated by MazF cleavage). Overall design: A + MazF and empty vector sample after 20 minutes of induction.