ena-PROJECT-Institute of Biology Leiden-21-10-2012-02:23:17:590-12
Root transcriptome sequencing of cultivated and wild carrots
Root transcriptomes of diverse cultivated and wild carrots were sequenced with Illumina Genome Analyzer IIx for 75 cycles. These include six varieties of cultivated carrot representing all European carrot root types and five wild carrot populations from widely dispersed sites. To involve more genetic diversity of each cultivated carrot variety or wild carrot population, three seedlings were randomly chosen from each cultivated carrot variety or wild carrot population. All plants were grown in the same climate chamber with the same growing condition. The results were used for SNP discovery, inferring the process of carrot domestication, and identifying domestication genes according to different gene expression patterns between cultivated and wild carrots.
Root transcriptomes of diverse cultivated and wild carrots were sequenced with Illumina Genome Analyzer IIx for 75 cycles. These include six varieties of cultivated carrot representing all European carrot root types and five wild carrot populations from widely dispersed sites. To involve more genetic diversity of each cultivated carrot variety or wild carrot population, three seedlings were randomly chosen from each cultivated carrot variety or wild carrot population. All plants were grown in the same climate chamber with the same growing condition. The results were used for SNP discovery, inferring the process of carrot domestication, and identifying domestication genes according to different gene expression patterns between cultivated and wild carrots.