PRJNA522677
Homo sapiens Genome sequencing
We performed a genome-scale screen to measure gRNA activity for two highly specific SpCas9 variants (eSpCas9(1.1) and SpCas9-HF1) and wild-type SpCas9 (WT-SpCas9) in human cells, and obtained indel rates of over 50,000 gRNAs for each nuclease, covering ~20,000 genes.