PRJNA557509
Metagenomics Reveals the Influence of pH and Phosphorus Content on the Bulk Soil Microbial Communities in Four Different Types of Cropping Systems
The abundance and composition of bulk soil bacterial communities are shaped by biotic and abiotic factors. Little is known to how cropping system at large-scale impact on bulk soil bacteria in maize producing region. We performed a large-scale longitudinal analysis of bacterial community and functional genes in the bulk soil under four cropping systems of maize across China using metagenomic and culture-based techniques. We found that the bacteria in bulk soil under various cropping system of maize clustered into respective groups, showing bacteria were shaped by cropping system to some extent. We further reveal that the strongest selection pressure on dominant bacteria was pH and phosphorus content under different cropping system. In these communities, 8% of dominant bacteria (141 taxonomic genera) consistently accounted for 64% of the soil bacterial communities across all samples. Pathway enrichment analysis showed that besides genetic information processing and metabolic pathways, high levels of phosphate metabolism related function genes such as phosphate abc transporter abundant in metagenome, suggesting a prevalent phosphate metabolism among bulk soil dominant bacteria. This work also generated a culture collection of 21,797 bacterial isolates, comprised of 55 genera of the dominant bacteria, and within which we identified phosphate-solubilizing strains, and plant growth promoting isolates.