Single_cell_transcriptome_of_schistosome_ovary_and_vitellaria_-sc-6879
Single_cell_transcriptome_of_schistosome_ovary_and_vitellaria_
Mice were infected with either mixed-sex cercariae or female cercariae generated by monomiracidia-infection of snails. Six weeks post-infection mature (paired) female worms or immature female worms were collected by portal perfusion of mice infected with mixed-sex cercariae or female cercariae, respectively. Ovaries and vitellaria were isolated from individual female worms following the protocol described by Hahnel et al, 2013 (PMID: 23936567) followed by dissociation into single cell suspensions. The live cells were sorted and prepared for 10X Chromium single cell sequencing (Diaz et al, 2020. PMDI: 33339816). The 10X single cell libraries were prepared and sequenced.    The samples for this study are:  FUGI_R_D7708903 (bisex ovaries)  FUGI_R_D7708518 (single-sex ovaries) FUGI_R_D7733089 (vitellocytes)     This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Schistosomiasis, a major neglected tropical diseases affecting the poorest regions in the world, is caused by the infection with parasitic flatworms of the genus Schistosoma. Schistosomes exhibit an exceptional reproductive biology since the sexual maturation of the female, which includes the differentiation of the reproductive organs, is controlled by pairing. The pathology associated with the infection is driven by the parasite eggs that get trapped in host tissues, mainly the liver and intestinal wall. Undertsanding the processes contributing to female maturation is not only of interest to the fundametal sexual biology of the parasite, but also critical to identify targets for novel control strategies for schistosomiasis. In this study, we isolated ovary and vitellaria tissue from both immature and mature female worms and employed single cell transcriptomics to identify specific signatures associated with the development of the female reproductive tissue triggered by pairing with the male.