XT_mutant_RNAseq-sc-2011-10-24T10:14:44Z-1720
XT_mutant_RNAseq
RNA was isolated from Xenopus tropicalis rax mutants and wild type siblings.  This was used to generate mutant and wild type RNA libraries for solexa sequencing.  The sequencing data will be compared to isolate changes that may be caused by loss of rax activity.  The RNA samples were extracted with Trizol, then were DNAse treated following the Invitrogen DNAse I protocol and re-precipitated with ethanol.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We have identified by tilling a Xenopus tropicalis rax mutant in which the gene has a premature stop codon before the homeobox domain.  The rax gene is essential for eye development and has been identified as a causative gene for human ocular malformations such as anophthalmia and microphthalmia.  The phenotype of the X.tropicalis mutant replicates the human condition with complete loss or small eyes.  By analysis of the full RNA complement of rax mutants compared to wild type siblings we hope to gain a better understanding of the role of rax during eye development and the genes that it regulates.  This is part of an ongoing collaboration with Dr Robert Grainger, University of Virginia. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see www.sanger.ac.uk/datasharing/