PRJNA771676
Comparison of PCR-free libraries
To enable comparision between NEBNext Ultra II, NEBNext Ultra II FS, and Illumina DNA PCR-Free library prep methods, sequencing libraries were prepared according the manufacturer recommendations from 250 ng of NA19240 cell line DNA and sequenced on a single NovaSeq 6000 S4 v1.5 flowcell (PE 2x150 bp). Reads were adapter trimmed (fastp 0.20.0), aligned) to the Telomere-to-Telomere chm13 reference (draft 1, bwa-mem 0.7.17), and duplicate marked (samblaster 0.1.24). 2.8B reads were randomly sampled from each bam (sambamba view -s 0.6.8).