DRP003562
Transcriptome analysis of Brachypodium distachyon treated with salicylic acid (SA) or acibenzolar-S-methyl (BTH)
We compared datasets of transcriptome in B. distachyon Bd21 leaves after 24 h treatment with 0.5 mM SA, 0.5mM BTH, or 0.5 % (v/v) dimethylsulfoxide. Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN), and its quality and quantity were validated by using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and a 2100 Bioanalyzer (Agilent). Libraries for RNA-seq were prepared for three biological replicates using the Truseq RNA library preparation kit (Illumina), and the libraries were sequenced using the Hiseq4000 sequencer (Illumina).
We compared datasets of transcriptome in B. distachyon Bd21 leaves after 24 h treatment with 0.5 mM SA, 0.5mM BTH, or 0.5 % (v/v) dimethylsulfoxide. Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN), and its quality and quantity were validated by using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and a 2100 Bioanalyzer (Agilent). Libraries for RNA-seq were prepared for three biological replicates using the Truseq RNA library preparation kit (Illumina), and the libraries were sequenced using the Hiseq4000 sequencer (Illumina).