ena-STUDY-PNRI-19-09-2014-22:43:33:135-358
RAD-seq genotyping of galactose-tolerant progeny from a cross between strains YO795 (EC-33) and YO796 (YPS163)
For Gal7-deletion yeast strains, growth on galactose-containing medium is lethal. We crossed 2 highly diverged S. cerevisiae strains with Gal7-deletions and selected the rare (~1/1000) progeny capable of growth on galactose. These strains were genotyped and karyotyped using RAD-seq (Restriction Associated DNA sequencing) with the MboI and MfeI restriction enzymes.
For Gal7-deletion yeast strains, growth on galactose-containing medium is lethal. We crossed 2 highly diverged S. cerevisiae strains with Gal7-deletions and selected the rare (~1/1000) progeny capable of growth on galactose. These strains were genotyped and karyotyped using RAD-seq (Restriction Associated DNA sequencing) with the MboI and MfeI restriction enzymes.