ena-STUDY-UNIVERSITI KEBANGSAAN MALAYSIA-10-10-2019-05:38:47:905-66
Ganoderma boninense NJ3 genome assembly was analyzed using Augustus gene prediction tool for the identification of carbohydrate-active enzyme and plant-host interaction genes.
In this study, comparative genome analysis was carried out using G. boninense NJ3 genome to identify and characterize carbohydrate-active enzyme (CAZymes) including cell wall degrading enzymes (CWDEs) in the fungal genome. Augustus pipeline was employed for gene identification in G. boninense NJ3 and the produced protein sequences were analyzed via dbCAN pipeline and PhiBase 4.5 database annotation for CAZymes and plant-host interaction (PHI) gene analysis, respectively. Comparison of CAZymes from G. boninense NJ3 was made against G. lucidum, a well-studied model Ganoderma sp. and five selected pathogenic fungi for CAZymes characterization. Functional annotation of PHI genes was carried out using Web Gene Ontology Annotation Plot (WEGO) and was used for selecting candidate PHI genes related to cell wall degradation of G. boninense NJ3. From this, datasets of G. boninense NJ3 Augustus gene annotation, GO annotation and protein ID from PhiBase 4.5 analysis were obtained that provided essential information of the extensive set of CAZymes-encoding genes that may directly contributed to the pathogenesis of G. boninense toward oil palm trees.
In this study, comparative genome analysis was carried out using G. boninense NJ3 genome to identify and characterize carbohydrate-active enzyme (CAZymes) including cell wall degrading enzymes (CWDEs) in the fungal genome. Augustus pipeline was employed for gene identification in G. boninense NJ3 and the produced protein sequences were analyzed via dbCAN pipeline and PhiBase 4.5 database annotation for CAZymes and plant-host interaction (PHI) gene analysis, respectively. Comparison of CAZymes from G. boninense NJ3 was made against G. lucidum, a well-studied model Ganoderma sp. and five selected pathogenic fungi for CAZymes characterization. Functional annotation of PHI genes was carried out using Web Gene Ontology Annotation Plot (WEGO) and was used for selecting candidate PHI genes related to cell wall degradation of G. boninense NJ3. From this, datasets of G. boninense NJ3 Augustus gene annotation, GO annotation and protein ID from PhiBase 4.5 analysis were obtained that provided essential information of the extensive set of CAZymes-encoding genes that may directly contributed to the pathogenesis of G. boninense toward oil palm trees.