UF Maize FSTs
Maize transposon flanking sequence tags from the UniformMu Robertson's Mutator tagging population
Approximately 85% of the reads correspond to bona fide transposon flanking sequence tags (FSTs). These FSTs have 31 bp at the start of each read with the following structure:  1) 4 bp barcode  2) 21 bp Mutator terminal inverted repeat primer (5'-ctcttcKtcYataatggcaat-3')  3) 6 bp of the Mutator element (5'-TATCTC-3')    The barcodes for each pool are as follows:  Row A CGAT  Row B CGTA  Row C CTAG  Row D CTGA  Row E TACG  Row F TCAG  Row G TCGA  Row H TCGT  Row I TCTG  Row J TGAG  Row K TGCA  Row L TGCT  Column 01 ACAG  Column 02 ACGA  Column 03 ACGT  Column 04 ACTG  Column 05 AGCA  Column 06 AGCT  Column 07 AGTG  Column 08 ATCG  Column 09 CACT  Column 10 CAGA  Column 11 CAGT  Column 12 CATG
Roberston's Mutator (Mu) transposon flanking sequence tags were sequenced from 144 transposon tagging lines from the maize UniformMu population (McCarty et al, 2005). TAIL-PCR products were amplified from 24 pools of 12 lines arranged in a 12 x 12 grid (Settles et al, 2004). The products were sequenced on GS-FLX 454 platform using custom adapter primers that gave directional reads from the transposon end as well as a 4 bp molecular bar code.