ena-STUDY-IMPERIAL COLLEGE LONDON-30-05-2019-16:25:39:995-611
This is RNA-seq from LUHMES cells and differentiated neurons with and without CSMD1 enhancer CRISPRi
We performed lentiviral CRISPR interference (CRISPRi) by recruiting dCas9 fused with the KRAB domain to the CSMD1 enhancer in the neuronal precursor cell line – Lund human mesencephalic (LUHMES). Given that the expression of CSMD1 was not detectable in LUHMES cells we differentiated these cells into neurons. To investigate the detailed molecular phenotype of CRISPRi at CSMD1, we performed RNAseq upon differentiation of neuronal precursors to neurons. RNAseq data shows significant up-regulation of CSMD1 in CRISPRi inactivated neurons as compared to controls.
We performed lentiviral CRISPR interference (CRISPRi) by recruiting dCas9 fused with the KRAB domain to the CSMD1 enhancer in the neuronal precursor cell line – Lund human mesencephalic (LUHMES). Given that the expression of CSMD1 was not detectable in LUHMES cells we differentiated these cells into neurons. To investigate the detailed molecular phenotype of CRISPRi at CSMD1, we performed RNAseq upon differentiation of neuronal precursors to neurons. RNAseq data shows significant up-regulation of CSMD1 in CRISPRi inactivated neurons as compared to controls.