PRJNA552583
In vivo and Ex vivo SHAPE-MaP structure probing of human lncRNA MEG3
This study reports in vivo and ex vivo SHAPE-MaP chemical probing analysis of human lncRNA MEG3 (maternally expressed gene 3). These techniques both compare the chemical reactivity of MEG3 folded in the cellular environment, either in the presence of protein partners (in vivo) or after gentle extraction and protein removal under experimental conditions that are not expected to denature the RNA secondary structure (ex vivo).