GSE161441
scRNA-seq of E18.5 small intestinal innate lymphoid cells
Embryonic innate lymphoid cells (ILCs) were isolated from small intestinal lamina propria to study their transcriptomic profile in the developing tissue. Overall design: ILCs were isolated from E18.5 small instestines of Rorc(gt)GFP/wt, Rorc(gt)GFP/GFP and Rorc(gt)GFP/GFP x Tbx21-/- (Hashtagged) and FACS-sorted as viable Lineage(CD19, CD3, CD5, F4/80,FceRIa, Gr-1)-CD45+CD122+ and/or CD127+ combined with the following 25 CITE-Seq antibodies: CD49b, NK1.1, DNAM1, CD3, CCR6, NKp46, CD117, Nrp-1, NKG2D, TCRgd, TCRb, CD49a, CXCR5,CD27, CD200R1, CD4, PD-1, CD127 PE + anti PE, a4b7 + anti-Biotin, ICOS, Sca-1, ST2, CD25, KLRG1