PRJNA430224
MinIon Nanopore Raw sequence reads for mixed populations of different Hepatitis B virus genotypes
Oxford Nanopore MinIon long read nanopore sequencing was performed on previously characterized HBV strain samples including either recombinant viruses, mixed populations of wild-type viruses and variants with major deletions, and mixed populations of single-nucleotide variants.The study concerns three previously characterized HBV-infected blood donor plasmas. Sample B5584 was infected with a 3,209-nt length genotype D1/E recombinant virus. Sample B6260 contained a complete 3,179-nt genotype D2 virus and a related 3,032-nt variant that harbored 123-nt and 24-nt deletions in the Pre-S1 and S regions, respectively. Sample B6505 contained a mixed population of 3,220-nt genotype A2 variants identified by sequence ambiguities within the "a-determinant" of the S gene, mutations that have been previously associated with vaccine escape and diagnostic failure.