Trachemys scripta embryonic transcriptome
Trachemys scripta elegans embryonic transcriptome
RNA was isolated from two Trachemys scripta (red-eared slider turtle) embryos (stage 14 and 17) in order to generate a T. scripta developmental transcriptome. mRNAs were reverse transcribed and the resulting cDNA pool was sequenced using an Illumina HiSeq 2000 generating 18.8Gb of sequence. Transcripts were assembled using Trinity (http://trinityrnaseq.sourceforge.net/) and annotated using Blast2GO (http://www.blast2go.com).