GSE161860
Oxidative Stress Impact on the Transcriptome of Differentiating Neuroblastoma Cells
Prenatal environmental exposures that have been shown to induce oxidative stress (OS) during pregnancy, such as smoking and alcohol consumption, are risk factors for the onset of schizophrenia and other neurodevelopmental disorders (NDDs). While the OS role in the etiology of neurodegenerative diseases is well known, its contribution to the genomic dysregulation associated with psychiatric disorders is less well defined. In this study we used SH-SY5Y cell line and applied RNA-sequencing to explore transcriptomic changes in response to OS before or during neural differentiation. We observed differential expression of many genes, most of which localised to the synapse and involved in neuronal differentiation. These genes were enriched in schizophrenia-associated signalling pathways, including PI3K/Akt, axon guidance, and signalling by retinoic acid. Interestingly, circulatory system development was affected by both treatments, which is concordant with observations of increased prevalence of cardiovascular disease in patients with NDDs. We also observed a very significant increase in the expression of immunity-related genes, supporting current hypotheses of immune system involvement in psychiatric disorders. These data suggest that early life exposure to OS has a disruptive influence on neuronal gene expression that may perturb normal differentiation and neurodevelopment, thereby contributing towards overall risk for developing psychiatric diseases. Overall design: Oxidative stress was chemically induced in  SH-SY5Y cells by the addition of 10mM hydrogen peroxide (H2O2) using two treatment protocols. In the first approach, the cells were simultaneously exposed to H2O2 and ATRA during the entire 7 days of differentiation (co-treatment protocol). In the alternative pre-treatment approach, cells were treated with 10mM H2O2 72 hours before commencement of the ATRA differentiation protocol. For each approach, there existed 3 replicates for peroxide-treated and 3 replicates for untreated samples.