PRJNA548840
Transcriptome analysis of root and shoot tissues under transient N-deprivation condition in wheat genotype (K9107) at seedling stage
Wheat genotype (K9107) grown in perlite and vermiculite homogenous mixture (perlite: vermiculite, 1:2 (v/v)) with nutrient MS (modified having no N source) with 4mM Calcium nitrate. After 14 days of growth period, half (in terms of number) of the seminal roots exposed under deprived external N-concentration (0.04mM calcium nitrate) while other half is under optimal N-concentration (4mM) for 6hrs, whereas in other sets whole seedlings were either exposed with deprived or optimal N-conditions. After 6hrs, root and shoot samples were harvested and store in RNAlaterTM (R0901 Sigma) for RNA sequencing consisting of total 14 libraries.