ena-STUDY-INRA (INRA PACA)-07-03-2019-08:50:37:207-246
Transcriptome-based exon capture of Harmonia axyridis
Two target enrichment experiments were performed with the same individuals, the first capture with 23 indexed multiplexed individuals and the second with a pool of 36 non-indexed individuals. Capture probes were designed for ~5 Mb of randomly chosen coding exonic regions of H. axyridis from a de novo transcriptome (Vogel et al. 2017). Target enrichment using SeqCap EZ Developer Library version 5.0 (Roche Nimbelgen Technologies). DNA extractions were performed using Qiagen Dneasy Blood & Tissue kit.  For more details, see https://www.biorxiv.org/content/10.1101/583534v1 .
Two target enrichment experiments were performed with the same individuals, the first capture with 23 indexed multiplexed individuals and the second with a pool of 36 non-indexed individuals. Capture probes were designed for ~5 Mb of randomly chosen coding exonic regions of H. axyridis from a de novo transcriptome (Vogel et al. 2017). Target enrichment using SeqCap EZ Developer Library version 5.0 (Roche Nimbelgen Technologies). DNA extractions were performed using Qiagen Dneasy Blood & Tissue kit.  For more details, see https://www.biorxiv.org/content/10.1101/583534v1 .