GSE201426
RNA sequencing of HeLa cells
DNA topoisomerase 3A (TOP3A) and mitochondrial DNA topoisomerase 1 (TOP1MT) play an important role in the maintenance and topological control of the mitochondrial DNA. In this study TOP3A, TOP1MT or both TOP3A and TOP1MT have been knocked down using siRNAs to determine the effect of the topoisomerases on mitochondrial transcription. RNA was isolated from ctrl siRNA, TOP3A siRNA, TOP1MT siRNA and TOP3A + TOP1MT siRNA transfected cells after 6 days of transfection. Overall design: 3 replicates of each of  ctrl siRNA, TOP3A siRNA, TOP1MT siRNA and TOP3A + TOP1MT siRNA treated