AT_rich_genome_sequencing-sc-2011-09-20T19:41:10Z-1935
AT_rich_genome_sequencing
Optimizing Illumina Next-Generation Sequencing library preparation for extremely AT-biased genomes
Genomes of some important pathogenic organisms like Plasmodium falciparum (high AT content) and Mycobacterium tuberculosis (high GC content) display extremes of base composition. The standard library preparation procedures that employ PCR amplification have been shown to cause uneven read coverage particularly across AT and GC rich regions, leading to problems in genome assembly and variation analyses. This study seeks to develop and optimize library-preparation procedures suitable for low quantity starting material and tolerant to extremely high AT content sequences.