GSE124402
LncRNA sequencing in human cardiac myocytes (HCMs), human cardiac fibroblasts-adult ventricular (HCF-av) and human cardiac microvascular endothelial cells (HCMECs) from a single fetal donor
lncRNA-sequencing were performed in three human heart-derived cell types Overall design: We report the application of sequencing technology for high-throughput profiling of lncRNA expressions in three human heart-derived cell types. In this project, rRNA was removed from the total RNA by ribosomal RNA removal kit, and the way of ion breaking were used to break the RNA into 200-300bp fragments. By using RNA as template, the first strand of cDNA was synthesized with 6 base random primers and reverse transcriptase. The first strand cDNA was used as the template to synthesize the second strand cDNA. When the second strand cDNA is synthesized, the base T is replaced by U to achieve the goal of a chain specific library. All of our RNA-seq projects used strand specific kits to construct the libraries. Finally, we show that lncRNA different expression in three human heart-derived cell types. This study provides abundance of lncRNAs that may predict cardiac diseases.