E-MTAB-9631
Transcription Termination and Antitermination of Bacterial CRISPR Arrays
V. cholerae A50 has a functional CRISPR-cas system with a conserved boxA sequence. Plasmids harboring protospacers that are perfect targets for each spacer of the array are introduced into wt and boxA mutant V. cholerae.  After a period of growth without selection, cells are collected and the protospacer plasmids are sequenced in a high throughput manner.
V. cholerae A50 has a functional CRISPR-cas system with a conserved boxA sequence. Plasmids harboring protospacers that are perfect targets for each spacer of the array are introduced into wt and boxA mutant V. cholerae.  After a period of growth without selection, cells are collected and the protospacer plasmids are sequenced in a high throughput manner.