GSE175979
polyA+ RNA seq of zebrafish gonad at 25 dpf and 30dpf
In this study, we explored the biological process of zebrafish gonad differentiation through Poly(A)-enriched RNA sequencing,and found that the differences in the gonads differentiating towards testis or ovary were represented through types and expression levels of different genes. Using the GO enrichment classification, it is found that, in the differentiating ovary, the differentially expressed genes are enriched in processes of material synthesis and metabolic pathway, indicating that oocyte requires the accumulation of maternal RNA, protein, sugar, lipids, vitamins and other substances, which is consistent with its basic function of providing primary materials for early development of fertilized embryo. In addition, during ovarian differentiation, the biological processes associated with the nucleus, mitochondria, and cytoplasmic particles are more active, and the genes associated with sperm egg recognition are also expressed during this period. Unlike the differentiating ovary, when the gonad differentiates into testis, the differentially expressed genes are more enriched in biological regulation and development processes, including morphogenesis, cell proliferation, etc.The cytometers adapted to these biological processes are associated with cell membrane, such as membrane receptors, extra-cellular regions, extra-cellular matrix, and cell connections, and also associated with nuclear chromatin. In addition, we enriched 14 signaling pathways involved in testis differentiation, and showed the expression heat map of every differentially expressed gene of each pathway. Overall, the study reveals the different biological processes existing in the differentiation of the zebrafish gonads into ovary or testis, helping us to get a comprehensive understanding of the basis on biological events and molecules during this process. Overall design: Zebrafish larvae were anesthetized in 0.2 mg/mL MS-222 and disected to isolate gonads. Each gonad is placed under a microscope to identify either the testis or the ovary. Six 25 dpf and six 30 dpf undamaged gonads with sound integrity were choosen for PolyA+ RNA-sequencing.