ena-STUDY-UNIVERSITY OF HELSINKI-15-01-2015-14:05:09:060-88
Lactococcus piscium MKFS47 genome sequencing and annotation
Lactococcus piscium strain MKFS47 is a psychrotrophic spoilage lactic acid bacterium, isolated from the cold-stored modified atmosphere packaged broiler filet strips with the first signs of spoilage. The genome was sequenced using 454Sequencer with GS Flx chemistry and Illumina HiScanSQ system. Reads were assembled using Newbler 2.0.00.20. The order of contigs was determined by PCR and gaps were filled by Sanger sequencing. CDSs were predicted using EasyGene and Prodigal and the gene functions were assigned by means of RAST and PANNZER software. Predicted gene coordinates and functions were manually reviewed.
Lactococcus piscium strain MKFS47 is a psychrotrophic spoilage lactic acid bacterium, isolated from the cold-stored modified atmosphere packaged broiler filet strips with the first signs of spoilage. The genome was sequenced using 454Sequencer with GS Flx chemistry and Illumina HiScanSQ system. Reads were assembled using Newbler 2.0.00.20. The order of contigs was determined by PCR and gaps were filled by Sanger sequencing. CDSs were predicted using EasyGene and Prodigal and the gene functions were assigned by means of RAST and PANNZER software. Predicted gene coordinates and functions were manually reviewed.