GSE210511
ER, mitochondria and ISR regulation by mt-HSP70 and ATF5 upon procollagen misfolding (E18.5 femur and tibia)
We used single cell RNA sequencing (scRNA-seq) to identify femur and tibia osteoblast cell stress response pathway to misfolding of type I procollagen with Gly610->Cys substitution in the triple helical region of alpha2(I) chain. Overall design: Femur and tibia cells were isolated from E18.5 embryos as described in Gorrell et al, Adv Sci, 2022