PRJNA672767
Amplicon sequencing data from PAM depletion experiments
Paired-end sequencing of PCR amplicons templated off of a PAM library target plasmid used in E. coli plasmid depletion assays for testing SpCas9 variants programmed with one of two different sgRNA spacers. The PAM library harbored a fully randomized 8-bp PAM region flanked by the two protospacers in appropriate orientations, as well as a distal, fully randomized 20-bp molecular identifier; each read pair captured the complete 8-bp PAM sequence and a non-contiguous region with part of the distal molecular identifier. Amplicons derived from both pre- and post-depletion library samples were sequenced.