E-MTAB-10692
RNAseq of Rhodococcus aetherivornas BCP1 in response to arsenic
To analyse the transcriptional response of Rhodococcus aetherivornas BCP1 to arsenic, RNA was extracted from BCP1 cultures exposed to arsenite [As(III)] 5 mM and arsenate [As(V)] 30 mM for 18 hours in the presence of glucose as only carbon and energy source. Control cultures were carried out without adding any arsenic oxyanions. Illumina Truseq stranded mRNA libraries were constructed after rRNA depletion via Illumina Ribozero and sequenced on Illumina HiSeq 1500 system 2 x 70nt PE rapid mode.
To analyse the transcriptional response of Rhodococcus aetherivornas BCP1 to arsenic, RNA was extracted from BCP1 cultures exposed to arsenite [As(III)] 5 mM and arsenate [As(V)] 30 mM for 18 hours in the presence of glucose as only carbon and energy source. Control cultures were carried out without adding any arsenic oxyanions. Illumina Truseq stranded mRNA libraries were constructed after rRNA depletion via Illumina Ribozero and sequenced on Illumina HiSeq 1500 system 2 x 70nt PE rapid mode.