GSE122542
HaloTag-based conjugation of proteins to barcoding-oligonucleotides
We describe the development of a high-sensitivity protein quantification system called HaloTag protein barcoding assay. The assay involves target protein linking to a unique molecule-counting oligonucleotide by click chemistry. Overall design: The assay involves target protein linking to a unique molecule-counting oligonucleotide by amido-bond or click chemistry.