GSE194388
Differential expression of extracellular vesicles-derived long RNA sequencing of plasma from patients after myocardial infarction with or without cardiac remodeling and healthy individuals
The goals of this study are to compare extracellular vesicles-derived circRNAs-lncRNAs-mRNA(RNA-seq) from the plasma of myocardial infarction patients with cardiac remodeling (C), without cardiac remodeling (NC), and normal healthy people (N). Human plasma was separated by centrifugation at 3,000 rpm for 10 min at room temperature (25°C) within 2 h after blood collection and then centrifuged at 13,000 rpm for 10 min at 4°C to remove debris. Isolated plasma samples were stored at -80°C until use. Extracellular vesicles RNA were isolated by affinity-based binding to spin columns using an exoRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany). RNA libraries were prepared for sequencing using SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. RNA-seq was performed by the Illumina NovaSeq 6000 on a 150-bp paired-end run. Overall design: Extracellular vesicles-derived long RNA profiles of patients after myocardial infarction with cardiac remodeling (C), without cardiac remodeling (NC) and normal healthy individuals (N) by deep sequencing using Illumina NovaSeq 6000