ena-STUDY-WASHINGTON UNIVERSITY  SCHOOL OF MEDICINE-25-11-2014-00:24:51:694-1710
Identification of microbial differences between IgA-High and IgA-Low wild-type mice in two facilities
Wild-type mice from specific-pathogen-free facilities were distinguished phenotypically by fecal IgA levels. Fecal samples were collected from IgA-High and IgA-Low mice from two separate facilities. Amplification of the V4 region of 16S rDNA was performed with individual barcodes for each sample, followed by pooling and sequencing on an Illumina MiSeq.
Wild-type mice from specific-pathogen-free facilities were distinguished phenotypically by fecal IgA levels. Fecal samples were collected from IgA-High and IgA-Low mice from two separate facilities. Amplification of the V4 region of 16S rDNA was performed with individual barcodes for each sample, followed by pooling and sequencing on an Illumina MiSeq.