PRJNA506108
Raw sequencing reads from amplicon-based quantitative sequencing of spacer acquisition by type I-F and I-E CRISPR-Cas systems in pestered cell populations
CRISPR-Cas systems rely on genetic memories, termed spacers, for sequence-specific immunity against phages and mobile genetic elements. Spacer diversity within communities is important for population-level immunity, but we have limited understanding of how this diversity is generated. Type I CRISPR-Cas systems use existing spacers to enhance the acquisition of new spacers through primed CRISPR adaptation (priming). We have developed an assay termed 'pestering' that mimics recurrent encounters with foreign genetic material. In this assay, cell populations are subjected to invasion by mobile plasmids through iterative conjugation. To quantify CRISPR adaptation, we developed a novel deep sequencing approach that allows normalisation between samples on a per cell basis and accounts for amplicon size biases. This dataset contains the sequencing data from this project.