DRP007286
16S rRNA pyrosequencing data of acetate-fed mice
To evaluate the effects of acetate on intestinal microbiota, colonic contents, mucus layer, FACS-sorted IgA-coated and noncoated bacteria from C57BL/6 mice, CD3e deficient mice, activation-induced cytidine deaminase deficient mice, and CCR6 deficient mice were sampled. Lysozyme, achromopeptidase and proteinase K were utilized for cell lysis. DNA fraction was collected via phenol/chloroform separation and ethanol precipitation. V4 region of 16S rRNA genes were amplified by PCR with dual barcoded primers. After DNA purification and quantification, pyrosequencing was done on MiSeq platform (Illumina, 2 ? 250 bp paired-end reads).