E-MTAB-9273
RNA-seq of human melanoma cell line A375m2 treated with SB202190 OR BIRB796 against DMSO-treated controls
Melanoma cells can switch between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype in response to signals from the environment. We found that p38 MAPK inhibitors SB202190 and BIRB796 induce phenotype switching from the dedifferentiated, invasive phenotype to the more differentiated phenotype in A375m2 melanoma cells cultured in three-dimensional collagen. The phenotype switch is accompanied by a morphological transition from rounded, blebby, amoeboid shape to elongated, spindle-like morphology. We performed transcriptome profiling with RNA-seq to uncover the underlying gene expression changes. Cells kept in 3D collagen were treated either with 10 µM inhibitor or with 0.1% DMSO (the vehicle) only for 48 hours and total RNA was isolated using a TRIzol-like procedure. Sequencing libraries were prepared from poly(A) RNA and sequenced with Illumina HiSeq 2500 instrument.
Melanoma cells can switch between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype in response to signals from the environment. We found that p38 MAPK inhibitors SB202190 and BIRB796 induce phenotype switching from the dedifferentiated, invasive phenotype to the more differentiated phenotype in A375m2 melanoma cells cultured in three-dimensional collagen. The phenotype switch is accompanied by a morphological transition from rounded, blebby, amoeboid shape to elongated, spindle-like morphology. We performed transcriptome profiling with RNA-seq to uncover the underlying gene expression changes. Cells kept in 3D collagen were treated either with 10 µM inhibitor or with 0.1% DMSO (the vehicle) only for 48 hours and total RNA was isolated using a TRIzol-like procedure. Sequencing libraries were prepared from poly(A) RNA and sequenced with Illumina HiSeq 2500 instrument.