ena-STUDY-Klinikum rechts der Isar, II. Medizinische Klinik-12-02-2020-13:12:33:847-282
PD-1-targeted immunotherapy in non-alcoholic steatohepatitis-induced liver cancer induces a pro-tumorigenic environment through CD8+ PD1+ T-cells.??
Mice with advanced non alcoholic fatty liver disease (NASH) by dietary feeding regime of choline deficient high fat diet (CD-HFD) were treated with antibodies (a-CD8 depletion; a-PD1 checkpoint inhibition; Co-depletion by a-CD8 and a-NK1.1 administration) for 8 weeks. Surprisingly, a-CD8 depletion prevented hepatocarcinogenesis; in contrast a-PD1 checkpoint inhibition induced hepatocarcinogenesis. By RNA-Seq of liver tissue, we want to investigate changes on RNA level induced by the different treatment regimes in the background of advanced NASH and hepatocarcinogenesis.
Mice with advanced non alcoholic fatty liver disease (NASH) by dietary feeding regime of choline deficient high fat diet (CD-HFD) were treated with antibodies (a-CD8 depletion; a-PD1 checkpoint inhibition; Co-depletion by a-CD8 and a-NK1.1 administration) for 8 weeks. Surprisingly, a-CD8 depletion prevented hepatocarcinogenesis; in contrast a-PD1 checkpoint inhibition induced hepatocarcinogenesis. By RNA-Seq of liver tissue, we want to investigate changes on RNA level induced by the different treatment regimes in the background of advanced NASH and hepatocarcinogenesis.