GSE129707
Dinucleotide regulation by REXO2 maintains promoter specificity in mammalian mitochondria
Oligoribonucleases are conserved enzymes that degrade short RNA molecules of up to 5 nt in length, and are assumed to constitute the final stage of RNA turnover. We have investigated the function of the mammalian oligoribonuclease REXO2 through a combination of biochemical, structural and animal model studies. We find that REXO2 is a specialised dinucleotide degrading enzyme that shows no preference between RNA and DNA dinucleotide substrates. This specialisation is explained by crystal structures of REXO2 bound to RNA or DNA substrates. REXO2 is an essential gene, while a heart and skeletal muscle-specific knockout mouse displays elevated dinucleotide levels and alterations in gene expression patterns indicative of aberrant dinucleotide-primed transcription initiation. We find that dinucleotides act as potent stimulators of mitochondrial transcription initiation in vitro, and conclude that efficient RNA turnover is required to maintain the promoter specificity of transcription in mammalian mitochondria. Overall design: Total RNA was isolated from heart tissue from 52 week old control (Rexo2loxP/loxP) and Rexo2 knockout mice (Rexo2loxP/loxP, +/Ckmm), TruSeq libraries produced in triplicate, sequenced and analysed for differential expression. Mitochondrial RNA was isolated from heart tissue from 52 week old control (Rexo2loxP/loxP) and Rexo2 knockout mice (Rexo2loxP/loxP, +/Ckmm), PARE libraries produced  in triplicate and sequenced for analysis of mitochondrial RNA processing.