44544a20-e49b-4b73-8839-5b433bee5d1d
CENH3 degradation based haploidization in Arabidopsis thaliana
The generation of haploid plants accelerates the crop breeding process. To extend the haploidization toolbox, we tested whether targeted in vivo degradation of CENH3 protein can be harnessed to generate haploids in Arabidopsis thaliana. We show that a recombinant anti-EYFP nanobody fused to either heterologous F-box (NSlmb) or SPOP/BTB ligase (SPOP) proteins can recognize maternally derived EYFP-tagged CENH3 in planta and make it accessible for the ubiquitin-proteasome pathway.
The generation of haploid plants accelerates the crop breeding process. To extend the haploidization toolbox, we tested whether targeted in vivo degradation of CENH3 protein can be harnessed to generate haploids in Arabidopsis thaliana. We show that a recombinant anti-EYFP nanobody fused to either heterologous F-box (NSlmb) or SPOP/BTB ligase (SPOP) proteins can recognize maternally derived EYFP-tagged CENH3 in planta and make it accessible for the ubiquitin-proteasome pathway.