ena-STUDY-UNIVERSITY HOSPITAL ZURICH-26-09-2019-10:56:51:049-25
Genome-wide maps of chromatin interactions with lncRNA H19X.
We identified lncRNA H19X as involved in the development of TGFb-mediated fibrosis. In order to understand H19X molecular mechanism of action we perform ChIRP-seq in a human fibroblasts cell line, BJ5TA. ChIRP-seq is an affinity capture assay that allows the identification of genomic binding sites for a specific lncRNA. We used two probe sets (even and odd) as internal control for each other. Telomerase RNA component (TERC) probes were used to validate the method. LacZ probes were used as negative control.
We identified lncRNA H19X as involved in the development of TGFb-mediated fibrosis. In order to understand H19X molecular mechanism of action we perform ChIRP-seq in a human fibroblasts cell line, BJ5TA. ChIRP-seq is an affinity capture assay that allows the identification of genomic binding sites for a specific lncRNA. We used two probe sets (even and odd) as internal control for each other. Telomerase RNA component (TERC) probes were used to validate the method. LacZ probes were used as negative control.