GSE182837
Role of Setd2 in regulatory T cells [H3K27ac CUT&Tag]
To determine the molecular regulation of Tregs by Setd2, large intestine Tregs (CD3+CD4+Foxp3-YFP+) from Setd2-deficient and control Tregs were subjected to H3K27ac CUT&Tag analysis. Overall design: Biological replicates of 60000 Tregs (CD3+CD4+Foxp3-YFP+) sorted by flow cytometry from the large intestine of 2 week old Foxp3Cre-YFPSetd2f/+ or Foxp3Cre-YFPSetd2f/f mice (n=3-5) were subjected to H3K27ac CUT&Tag analysis.