STUDY_CMX_464G57RFW
Amplicon sequencing of 60 coral samples from Papua New Guinea
We chose a combination of 3 marker genes targeting the coral animal host, its symbiotic alga, and the associated bacterial microbiome, to characterize 60 coral colonies collected during the Tara Pacific expedition and processed in situ, onboard the research vessel Tara. We used Oxford Nanopore Technologies (ONT) to sequence marker gene amplicons. We re-sequenced the same PCR amplicons using Illumina technologies to test the accuracy of Nanopore technology.
We chose a combination of 3 marker genes targeting the coral animal host, its symbiotic alga, and the associated bacterial microbiome, to characterize 60 coral colonies collected during the Tara Pacific expedition and processed in situ, onboard the research vessel Tara. We used Oxford Nanopore Technologies (ONT) to sequence marker gene amplicons. We re-sequenced the same PCR amplicons using Illumina technologies to test the accuracy of Nanopore technology.