Generating_Primaquine_resistant_P__berghei-sc-4760
Generating_Primaquine_resistant_P__berghei
Mice infected with P. berghei were treated with sub-curative doses of Primaquine (PQ) over 8 passages. Unlike other similar drug pressure assays performed at Imperial (e.g Lumefantrine), cloned parasites with confirmed resistance to PQ also showed an inability to transmit to mosquitoes. It is hypothesized that PQ resistance mode-of-action is linked to the production of non-viable gametocytes (similar to atovaquone which was recently reported). Consequently, evolution of PQ resistance is hypothesized to be self-limiting as it cannot escape the mammalian host and complete life cycle in the mosquito.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Resistance to Primaquine (PQ) appears to simultaneously prevent parasite transmission in P. berghei. Identification of the genetic basis of this will shed light on PQ mode-of-action and help understand how/whether PQ drug resistance may evolve in the field.