GSE126019
NGS of 3'-termini of tRNA from E. coli
To avoid biases from secondary structure and modifications we sequenced the hypomodified 3'-terminus of tRNA This allowed assessment of relative levels of mature tRNA as well as low abundant fractions like non-intact tRNA or precuresor tRNA Overall design: Purified tRNA from E. coli (wt, cca K.O., rnt K.O.) from exponential (exp) and stationary (stat) phase was analysed