Sapelo Island Bacterioplankton Metatranscriptome
Metatranscriptomic Analysis of Bioreactive DOC in Coastal Seawater
Two replicate coastal seawater samples were collected and immediately filtered for RNA extraction of bacterioplankton communities. Two additional samples were first amended with phytoplankton- or vascular plant-derived dissolved organic matter for one hour prior to filtration and extraction. Sampling was carried out in May 2007 at the University of Georgia Marine Institute on Sapelo Island, located at latitude +31.3822 and longitude -81.2803. The extracted RNA was enriched in mRNA, linearly amplified, and pyrosequenced by GS FLX LR70.   <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=&db=Nucleotide&cmd=search&term=FJ744762:FJ745272[accn]" >FJ744762-FJ745272 </a> are ribosomal RNA sequences associated with this project.