UPSC-0181
Exploring the ß-tubulin gene family in a benzimidazole-resistant Parascaris univalens population
Benzimidazole (BZ) drugs are frequently used to treat infections with the equine ascarid Parascaris univalens due to increasing resistance to macrocyclic lactones and pyrantel. Benzimidazole resistance is rare in ascarids, in contrast to strongyle parasites where this resistance is widespread. In strongyles, single nucleotide polymorphisms (SNPs) at codons 167, 198 and 200 in a ß-tubulin gene have been correlated to BZ resistance, but little is known about the ß-tubulin genes and their possible involvement in BZ resistance in P. univalens and other ascarids. Previously two ß-tubulin genes (isotypes) have been identified in P. univalens. In this study, we present five additional ß-tubulins as well as the phylogenetic relationship of all seven genes to ß-tubulins of other nematodes. In addition, the efficacy of fenbendazole (FBZ) for treatment of P. univalens on a Swedish stud farm was studied in 2019 and 2020 using faecal egg count reduction test. Reductions varied from 73 % to 88 %, indicating the presence of a resistant P. univalens population on the farm. The emergence of BZ resistance emphasize the need for development of molecular markers for rapid and more sensitive detection of resistant populations. We therefore investigated whether possible SNPs at positions 167, 198 or 200 in any of the ß-tubulin genes could be used to distinguish between resistant and susceptible P. univalens populations. Amplicon sequencing covering the mutation sites 167, 198 and 200 in all seven ß-tubulin genes revealed an absence of SNPs in both resistant and susceptible populations, suggesting that the mechanism behind BZ resistance in ascarids is different from that in strongyle nematodes and the search for a molecular marker for BZ-resistance in P. univalens needs to continue.