GSE105515
H3K9Me3 marks in KAP1 deficient regulatory T cells
We aim to determine the role of KAP1 in regulatory T cells. Sicne KAP1 recruits H3K9 specific methyltransferase SETDB1, we focused on H3K9me3. Although H3K9me3 marks aroung transcription start site were dramatically reduced, this change did not correlated with the change of transcription levels between KAP1 sufficient and deficient Tregs determined by RNA-seq. Our data suggest the transcritpion regulation by KAP1 in regulatory T cells is not dependent on H3K9me3 marks. Overall design: KAP1 sufficient and deficient Tregs are sorted and the chromatin was immunoprecipitated with anti-H3K9Me3 antibody. The precipitated chromatin was sequenced by Hiseq2500.