GSE37799
GSE37799: Identification of Transcription Factor Ppie1_EFL1::GFP Binding Regions in YA
Summary: modENCODE_submission_3072  This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648  Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals.  Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.  For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf  Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: YL445(official name : YL445 genotype : unc119(ed3); vrIs81 [pPIE-1::EFL-1::GFP FLAG: EFL-1 3?UTR genotype : unc-119 (+)] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description :  made_by :  ); Developmental Stage: Young Adult; Genotype: unc119(ed3); vrIs81 [pPIE-1::EFL-1::GFP FLAG: EFL-1 3?UTR; Sex: Hermaphrodite;  EXPERIMENTAL FACTORS: Developmental Stage Young Adult; Target gene efl-1; Strain YL445(official name : YL445 genotype : unc119(ed3); vrIs81 [pPIE-1::EFL-1::GFP FLAG: EFL-1 3?UTR genotype : unc-119 (+)] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description :  made_by :  ); temp (temperature) 20 degree celsius