GSE174127
10X Genomics Single Cell RNAseq of wild-type Ciona robusta embryos at 12 hours post fertilization (hpf)
We used 10X Genomics Chromium system to profile single cells from a pool of Wild-type Ciona robusta embyros at 12 hours post fertilization (hpf) Overall design: A few hundred wild-type Ciona intestinalis embryos were collected at 12 hours post fertilization (hpf). Embryos were dissociated and loaded into 10X Chromium cell controller, single-cell library was prepared using Chromium Single Cell 3' v2 Reagent kit following the manufacturer's manual.