GSE114502
Altered mRNA splicing by mutant p53 activates oncogenic RAS in pancreatic cancer
Integrating transcriptomic analyses of both clinical PDAC and PDAC models, we identified that the most common neomorphic TP53 mutation (p53R175H) rewires RNA splicing promoting transcriptome-wide retention of cassette exons bearing cytosine(C)-rich sequences. Overall design: Examination of splicing changes attributed to p53R175H in  patient derived organoids (HT42), murine pancreatic cancer cell line (KPC), murine pancreatic intraepithelial neoplasia (PanIN).