ena-STUDY-JIC-18-09-2014-15:02:04:136-272
John Innes Centre grassland soil metagenome amplicon study (V1-V2 16S and ITS1)
Soil microbial John Innes Centre Church Farm grassland metagenome based on bacterial V1-V2 fragment of 16S rRNA and  ITS1 for fungi. Primers were obtained from Eurofins, Germany. Barcodes comes from the Roche MID collection. PCR efficiency was quantified using Qiagen QIAxcel Advanced System, purified using Qiagen PCR purification kit and pooled in equimolar concentration (for sand bacteria:fungi 1:1, for compost bacteria:fungi 3:1)
Soil microbial John Innes Centre Church Farm grassland metagenome based on bacterial V1-V2 fragment of 16S rRNA and  ITS1 for fungi. Primers were obtained from Eurofins, Germany. Barcodes comes from the Roche MID collection. PCR efficiency was quantified using Qiagen QIAxcel Advanced System, purified using Qiagen PCR purification kit and pooled in equimolar concentration (for sand bacteria:fungi 1:1, for compost bacteria:fungi 3:1)