GSE196186
Insight into the potential candidate genes and signaling pathways involved in lymphoma progression in dogs using a comprehensive whole blood transcriptome analysis
Purpose: This goal of this study was to explore the important genes and pathways involved in canine lymphoma progression and understand the underlying molecular using RNA-Sequencing. Methods: RNAs acquired from seven pairs of lymphoma and non-lymphoma blood samples were sequenced from different breeds of dogs. Sequencing reads were preprocessed, aligned with the reference genome, assembled and expressions were estimated through bioinformatics approaches. Result: A total of 625 differentially expressed genes (DEGs) were identified between lymphoma and non–lymphoma samples, including 347 up–regulated DEGs such as SLC38A11, SCN3A, ZIC5 etc. and 278 down–regulated DEGs such as LOC475937, CSMD1, KRT14 etc. Especially, the pathway of PI3K-Akt signaling, which was directly related to the lymphoma, was enriched in KEGG analysis. In the protein-protein interaction network, CDK1 was found to be a top hub gene with highest degree of connectivity in lymphoma samples. Conclusion: We found important candidate genes and pathways for further testing in canine lymphoma. Overall design: Blood transcriptome profiles of lymphoma and non-lymphoma disease of canine