GSE159719
RNA structure probing to characterize RNA-protein interations on a low abundance pre-mRNA in living cells
We develop an enrichment strategy to enriched for low abundance RNAs of interest before reverse transcription for RNA struture probing experiments Overall design: Mbnl1-/-,Mbnl2-/- double knockout mouse embryonic fibroblasts were treated with 2'-methylnicotinic acid imidazolide (NAI) or DMSO only and used SHAPE-MaP to examine the structure of regulatory region of a low abundance pre-mRNA. Target gene chromosome coordinates (mm10): Rn7SK: chr9:78175303-78175633 Rpph1: chr14:50807449-50807767 Mbnl1: chr3:60614481-60614939