GSE162596
Genome resequencing data of adaptive laboratory evolved E. coli W
Using a synthetic biosensor to couple production of a specific metabolite with cell growth, we spontaneously evolved cells under the selective condition toward the acquisition of genotypes that optimally reallocated cellular resources. Using 3-hydroxypropionic acid (3-HP) production from glycerol in Escherichia coli as a model system, we found spontaneous mutations in the conserved regions of AC and CRP. Overall design: Comparison of genomic DNA between parental and evolved E. coli W strain