GSE201091
Aortic single-cell RNA sequencing in ACTA2 R179 variants
Single-cell RNA datasets for aortic tissue from 8-week-old inducible smooth muscle cell-specific knock-in of Acta2 R179C mutation vs. littermate controls and aortic aneurysm tissue from a human patient carrying an ACTA2 R179H variant vs. a pediatric organ donor healthy control. Overall design: Aortic tissue from 8-week-old control and smooth muscle cell-specific Acta2R179C knock-in mice was enzymatically digested and processed for single-cell RNA sequencing using 10X Genomics kit and pipeline. Separately, human aortic tissue was obtained from a young child with aortic aneurysm and ACTA2 R179H variant and discarded aortic tissue from a pediatric heart donor and similarly digested and processed.