DRP006523
UPA-Seq using subcellular fractions of neurons
Investigating the sensitivity of lncRNAs to short UV irradiation in cultured hippocampal neurons. UV-irradiated neurons were fractionated into cytosol, and synaptosomes. RNAs were purified from each fraction using Trizol, and subsequently used for RNA-Seq analyses. Ribosomal RNAs were removed using ribozero kit before the library preparation.