CTTV020_Epigenomes_of_Cell_Lines_ATAC_seq-sc-4031
CTTV020_Epigenomes_of_Cell_Lines_ATAC_seq
These ATAC-seq experiments are carried out as part of the CTTV020 project to identify an epigenetic signature of common immortalised cell lines / models. This signature / fingerprint will be used in combination with data from RNAseq and ChIPseq experiments from the same cell types to compare various human immortalised cell  models against primary cells and tissues.  The overall aims of the CTTV020 Cell Line Epigenome project are to establish a systematic approach for the determination of human biological and disease relevance through the generation of transcriptomic and epigenomic data in cell types of interest. * Integration of cell type epigenome and transcriptome data with existing and newly generated reference data from human tissue and cell types to identify assay systems which will provide greater confidence in translating target biology and compound pharmacology to patients. * To provide a framework for the identification of optimal cell types for target identification / validation studies and drug discovery.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
There is currently a drive to establish cell based assay systems of greater human biological and disease relevance through the use of well characterised transformed cell lines, primary cells and complex cellular models (e.g. co-culture, 3D models). However, although the field is gaining valuable experience in running more non-standard & complex cell assays for target validation and compound pharmacology studies, there is the lack of a systematic approach to determine if this expansion in cell assay models is reflected in increased human biological and disease relevance. The increasing wealth of publically available transcriptomic, and epigenome (ENCODE and Epigenome Roadmap) data represents an ideal reference mechanism for determining the relationship between cell types used for target & compound studies to primary human cells and tissues from both healthy volunteers & patients. The CTTV020 epigenomes of cell line project aims to generate epigenetic and transcriptomic profiles of cell lines and compare these with existing and newly generated reference data sets from human tissue and cell types. The aim is to identify assay systems which will provide greater confidence in translating target biology and compound pharmacology to patients.  Multiple cell types commonly used within research have been grouped according to biology. Examples include erythroid, lung epithelial, hepatocyte cell types and immortalised models of monocyte / macrophage biology.