PRJNA435777
RNA-Seq of Wheat (Triticum aestivum)
Total RNA libraries were prepared using isolated total RNA according to the protocol of Illumina TrueSeq RNA Library Preparation Kit (https://support.illumina.com/). The libraries were checked for the average size (300-700 bp) on Agilent DNA 1000 chip using Agilent 2100 Bioanalyzer. After obtaining the Qubit concentration for each of the respective libraries, cluster generation was performed using Illumina CBotinstrument and finally loaded onto Illumina HiSeq platform for paired-end sequencing.