GSE172485
KRAS and MEK activation promote antisense oligonucleotide uptake and activity by limiting collagen production
Using RNAseq we describe transcriptional changes downstream of MEK1 affecting RNA kncokdown following free uptake RNaseH1 gapmer antisense oligonucleotides Overall design: We perform RNAseq on cell lines with a stimulated MAPK pathway via inducible expression of KRAS(G12D), inducible expression of MEK1(S218/S222DD) and by stimulation with the PKC activator, PMA