GSE177030
Single-cell transcriptome and cell-specific network analysis reveal the reparative effect of NT-4 supplementation in preantral follicles grown in vitro [gc_RNA-seq]
In-vitro-grow (IVG) of preantral follicles is essential for female fertility preservation, while effective approach to improve it is far from being explored. Here, we report the dynamic location of TRKB in human and mouse ovaries, and a positive relation between human oocyte maturation and NT-4 level in follicular fluid. For the first time, we observe the improving effect of NT-4 on mice preantral follicle IVG, including follicle diameter, hormone production and oocyte maturation. Single-cell transcriptome analysis shows that the reparative effect of NT-4 on oocyte maturation in IVG may be mediated by regulation of PI3K-Akt signaling pathway and subsequent organization of F-actin. Suppression of advanced stimulated complement system in granulosa cells may also contribute to the improvement. Cell-specific network analysis reveals that NT-4 may recover the inflammation damage induced by abnormal lipid metabolism in IVG environment. Collectively, our data suggest that NT-4 is involved in the ovarian physiology and may serve to improve the efficiency of preantral follicle IVG for fertility preservation. Overall design: Granulosa cell mRNA profiles of 12 to 14-day old Kunming mice; Granulosa cells were derived from preantral follicle grown in vitro ( with or without NT-4 ) for 6 days.