Nuclesome positioning
Genome-wide identification of nucleosome positions in fission yeast
Fission yeast mononucleosomes were prepared as described in Lantermann et al. (Methods 48, 3 218-225, 2009) with minor changes.  Mononucleosomal libraries were prepared and sequenced by Illumina sequencing according to the manufactures instructions.
We mapped nucleosomes in three species of fission yeast--S. pombe, S. octosporus and S. japonicus--by deep sequencing MNase-digested mononucleosomal DNA for comparative studies.