PRJNA737449
Sequencing of ShCAST RNA-guided in vitro transposition products
Total DNA sequencing of pools of in vitro transposition products in plasmid (pTarget_CAST) using the ShCAST Tn7-CRISPR-Cas transposition system. Investigating role of TnsC ATPase in target-site selection through use of non-hydrolyzable ATP analog and mutant alleles. Mechanistic insights are relevant to genome editing applications.