GSE99928
The physiology of Agaricus bisporus in semi-commercial compost cultivation appears to be highly conserved among unrelated isolates
The white button mushroom Agaricus bisporus is the most widely produced edible fungus with a great economical value. Its commercial cultivation process is often performed on wheat straw and animal manure based compost that mainly contains lignocellulosic material as a source of carbon and nutrients for the mushroom production. As a large portion of compost carbohydrates are left unused in the current mushroom cultivation process, the aim of this work was to study wild-type A. bisporus strains for their potential to convert the components that are poorly utilized by the commercial strain A15. Growth profiling suggested different abilities for several A. bisporus strains to use plant biomass derived polysaccharides, as well as to transport and metabolize the corresponding monomeric sugars. Six wild-type isolates with diverse growth profiles were compared for mushroom production to A15 strain in semi-commercial cultivation conditions. Transcriptome and proteome analyses of the three most interesting wild-type strains and A15 indicated that the unrelated A. bisporus strains degrade and convert plant biomass polymers in a highly similar manner. This was also supported by the chemical content of the compost during the mushroom production process. Our study therefore reveals a highly conserved physiology for unrelated strains of this species during growth in compost. Overall design: The aim of the study was to compare the gene expression of different strains of A. bisporus (A. bisporus strain A15, 065 BP-8, 219 30P and 245 AMA-7) at different stages of its life cycle to select for better abilities towards components that are poorly utilized by the commercially cultivated strain A15. The samples were taken at primordial stage and first flush respectively.