E-MTAB-10161
RNA sequencing of HEK293T cells expressing peptide 9J10, a 14-3-3 interacting peptide
We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes.  The peptide was identified in a screen for FOXO3a reactivation and has been shown to interact with 14-3-3 proteins and induce relocalisation of FOXO3a from the cytoplasm to the nucleus.  A peptide with a single amino acid mutation does not interact with 14-3-3 and was included as a control.  A constitutively active FOXO3a mutant was also included as a control.
We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes.  The peptide was identified in a screen for FOXO3a reactivation and has been shown to interact with 14-3-3 proteins and induce relocalisation of FOXO3a from the cytoplasm to the nucleus.  A peptide with a single amino acid mutation does not interact with 14-3-3 and was included as a control.  A constitutively active FOXO3a mutant was also included as a control.