ena-STUDY-UNIVERSITY OF DUISBURG-ESSEN-18-06-2021-09:12:46:890-1030
Standardized high-throughput biomonitoring using DNA metabarcoding: Strategies for the adoption of automated liquid handlers
Contrary to traditional methods, DNA metabarcoding can be upscaled to process hundreds of samples in parallel. However, while automated high-throughput analysis workflows are well-established in the medical sector, manual sample processing still predominates in biodiversity monitoring laboratory workflows limiting the upscaling and standardization of DNA metabarcoding for routine monitoring. Here we present a complete, automated, scalable, and reproducible DNA metabarcoding workflow to extract DNA from bulk samples, perform PCR and library preparation on a single high-throughput liquid platform deck layout.
Contrary to traditional methods, DNA metabarcoding can be upscaled to process hundreds of samples in parallel. However, while automated high-throughput analysis workflows are well-established in the medical sector, manual sample processing still predominates in biodiversity monitoring laboratory workflows limiting the upscaling and standardization of DNA metabarcoding for routine monitoring. Here we present a complete, automated, scalable, and reproducible DNA metabarcoding workflow to extract DNA from bulk samples, perform PCR and library preparation on a single high-throughput liquid platform deck layout.