ena-STUDY-UNIVERSITY OF BRISTOL-24-08-2020-20:46:08:241-274
Englacial water can slowly freeze in the englacial channels where the slow freezing excludes air bubbles giving the ice a clear aspect. This ice is then uplifted to the surface ablation zone of the glacier by glacial ice movements and can therefore be seen on the glacier surface in the form of clear ice bands. We employed an indirect method to sample englacial water by coring these ice bands. We studied 62 ice samples collected from clear-ice bands (i.e. frozen englacial water) and cloudy ice (i.e. meteoric deposition) in the Storglaciären (Sweden). We characterized microbial communities found inside the different ice types through the sequencing of the 16S rRNA hypervariable V3-V4 region of Bacteria and Archaea, sequenced using Illumina MiSeq platform (2x300 bp) with Pro341F and Pro805R primers.
Englacial water can slowly freeze in the englacial channels where the slow freezing excludes air bubbles giving the ice a clear aspect. This ice is then uplifted to the surface ablation zone of the glacier by glacial ice movements and can therefore be seen on the glacier surface in the form of clear ice bands. We employed an indirect method to sample englacial water by coring these ice bands. We studied 62 ice samples collected from clear-ice bands (i.e. frozen englacial water) and cloudy ice (i.e. meteoric deposition) in the Storglaciären (Sweden). We characterized microbial communities found inside the different ice types through the sequencing of the 16S rRNA hypervariable V3-V4 region of Bacteria and Archaea, sequenced using Illumina MiSeq platform (2x300 bp) with Pro341F and Pro805R primers.