PRJNA415429
Elucidation of PAM requirements of Cas12a proteins from Eubacterium coprostanoligenes, Francisella tularensis subsp. novicida, Moraxella bovoculi, Moraxella caprae, Prevotella bryantii, Proteocatella sphenisci Other
A cell-free transcription-translation (TXTL) system was used to rapidly characterize the protospacer-adjacent motif (PAM) requirements of diverse Cas12a (Cpf1) proteins. We simultaneously expressed the Cas protein and its crRNA in a solution containing a PAM library and monitored cleavage of the library using high-throughput sequencing.