GSE174483
Nuclear and cytoplasmic whole transcriptome RNA-sequencing of mouse endothelial cell line and mmu-miR-466c knock-down cell line
To analyze the role of miR-466c in the regulation in hypoxic response, we used CRISPR-mediated gene editing to remove 97 bp region from the Sfmbt2 intron 10 which contains the miR-466c hairpin. We performed whole transcriptome RNA-sequencing of the parental mouse endothelial cell line C166 and the mmu-miR-466c knock-down cell line. Overall design: Two cell lines (C166 wildtype and C1.1 mutant cell lines) were cultured in normoxia or hypoxia (2h and 24h timepoints) and split to nuclear and cytoplasmic fractions. All samples were done in triplicate.