GSE131868
RNA sequencing of mouse hepatic response to compounds with predicted lifespan-extending effect
This dataset consists of hepatic gene expression profiles of mice subjected to 4 compounds predicted by Connectivity Map (CMap) using gene signatures identified for known lifespan-extending interventions: ascorbyl-palmitate, KU-0063794, AZD8055 and rilmenidine. Corresponding age-, sex- and strain-matched littermate controls are also presented.  Using this data, we confirmed the association between longevity signatures and gene expression response to the predicted compounds in vivo, using the same mouse model as for the identification of gene signatures associated with lifespan extension. This pilot project demonstrated the applicability of such approach for the discovery of new interventions with a desirable effect on gene expression and provided appealing candidates for further longevity studies. Overall design: 3-month old UM-HET3 male mice from University of Michigan Medical School were subjected to ascorbyl-palmitate (6.3 ppm), KU-0063794 (10 ppm), AZD8055 (20 ppm) and rilmenidine (10 ppm) for 1 month. Their littermate controls of the same age and sex were also used for the analysis.  4 biological replicates were used for each treated group, while control samples included 8 biological replicates (24 samples in total). In all cases, interventions continued until the animals were sacrificed. RNA was extracted from liver tissues with PureLink RNA Mini Kit as described in the protocol and passed to CEL-seq.