ena-STUDY-SC-15-06-2018-13:28:30:685-684
Dracula fish Danionella dracula genome assembly fDanDra1.1
The Vertebrate Genomes Project at the Sanger Institute and the University of Cambridge, UK provides high quality reference genome assemblies as part of the Genome10K initiative. This project provides the genome assembly of Danionella dracula. The assembly fDanDra1.1 is based on ~60x PacBio Sequel data, and ~65x Illumina HiSeqX data generated from a 10X Genomics Chromium library. An initial PacBio assembly was made using Falcon-unzip. The primary contigs were first scaffolded using a wtdbg assembly, then scaffolded further using the 10X data with scaff10x. After using the PacBio data to gap fill with PBJelly and polish with Arrow, the assembly was polished again using the 10X Illumina data and freebayes. Retained haplotigs were identified with purge_haplotigs. Finally, the assembly was manually assessed using gEVAL.
The Vertebrate Genomes Project at the Sanger Institute and the University of Cambridge, UK provides high quality reference genome assemblies as part of the Genome10K initiative. This project provides the genome assembly of Danionella dracula. The assembly fDanDra1.1 is based on ~60x PacBio Sequel data, and ~65x Illumina HiSeqX data generated from a 10X Genomics Chromium library. An initial PacBio assembly was made using Falcon-unzip. The primary contigs were first scaffolded using a wtdbg assembly, then scaffolded further using the 10X data with scaff10x. After using the PacBio data to gap fill with PBJelly and polish with Arrow, the assembly was polished again using the 10X Illumina data and freebayes. Retained haplotigs were identified with purge_haplotigs. Finally, the assembly was manually assessed using gEVAL.