E-MTAB-5480
Single cell RNA-sequencing of Spike-ins and Control RNA using 10x Genomics Chromium system
In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. In this dataset, we assess the RNA detection rates using high-throughput 10x Genomics Chromium system. We mix equal volume of Control Brain RNA (3µl; FirstChoice Total Brain RNA; #AM7962) and ERCC spikes (3µl 1:4 dilution; #4456653) to make a ‘2x Control RNA+ERCC’ master mix. The ‘2x Control RNA+ERCC’ master mix is diluted with equal volume nuclease-free water to make ‘1x Control RNA+ERCC’ master mix. 3µl of ‘1x Control RNA+ERCC’ master mix is added to Chromium single cell suspension and processed as per Chromium guidelines.
In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. In this dataset, we assess the RNA detection rates using high-throughput 10x Genomics Chromium system. We mix equal volume of Control Brain RNA (3µl; FirstChoice Total Brain RNA; #AM7962) and ERCC spikes (3µl 1:4 dilution; #4456653) to make a ‘2x Control RNA+ERCC’ master mix. The ‘2x Control RNA+ERCC’ master mix is diluted with equal volume nuclease-free water to make ‘1x Control RNA+ERCC’ master mix. 3µl of ‘1x Control RNA+ERCC’ master mix is added to Chromium single cell suspension and processed as per Chromium guidelines.