DRP000384
Rattus norvegicus Long Evans transcriptome project
Left and right rat (postnatal 5 weeks) dorsal hippocampal CA3 region. 3 sets of paired (left vs. right CA3) samples are made. The number of animals used in each sample is 36, 57, and 34. Total RNA amount of 3.0 ug per sample was used. Small RNAs were purified using a miRNeasy (Qiagen) kit. Small RNA cDNA library was constructed according to Kawano et al (2010) Biotechniques, 49: 751-755. The number of PCR cycles for small RNA cDNA library construction is 12. The concentration of each purified small RNA cDNA library was determined using Agilent 2100 BioAnalyzer with a DNA1000 Labchip (Agilent) and adjusted to 6 pM for sequencing. The small RNA cDNA libraries were sequenced using Genome Analizer IIx.