ena-STUDY-Lorraine University-26-07-2021-15:07:10:893-581
RMS analysis of human cell lines
Epitranscriptomic modifications of stable RNAs are dynamically regulated and specific profiles of 2'-O-methylation in rRNA have been associated to distinct cancer types. However, these observations pointed out the existence of at least two distinct groups: a rather large group with constitutive rRNA Nm residues exhibiting a stable level of methylation and a more restricted set of highly variable modifications, giving rise to the concept of “specialized ribosomes”. These heterogenous ribosomes can modulate their translational properties and be key regulatory players, depending on the physiological state of the cell. However, these conclusions were drawn from a limited set of explored human cell lines or tissues, mostly related to cancer cells of the same type. Here we report a comprehensive analysis of human rRNA Nm modification variability observed for >15 human cell lines grown in different media and conditions. Our data demonstrate that human Nm sites can be classified into four groups, depending on their observed variability. About 1/3 of rRNA 2'-O-methylations are invariably modified at the same level in all tested samples, the second relatively stable group of constitutive modifications (another ½ of the total) shows a slightly higher variance and two variable groups, showing an important heterogeneity. Mapping of these four classes on the human ribosome 3D structure shows that stable positions are preferentially located in the important ribosome functional sites, while variable and highly variable residues are mostly distributed to the ribosome periphery. Possible relationships of such stable and variable modifications to the ribosome functions are discussed.
Epitranscriptomic modifications of stable RNAs are dynamically regulated and specific profiles of 2'-O-methylation in rRNA have been associated to distinct cancer types. However, these observations pointed out the existence of at least two distinct groups: a rather large group with constitutive rRNA Nm residues exhibiting a stable level of methylation and a more restricted set of highly variable modifications, giving rise to the concept of “specialized ribosomes”. These heterogenous ribosomes can modulate their translational properties and be key regulatory players, depending on the physiological state of the cell. However, these conclusions were drawn from a limited set of explored human cell lines or tissues, mostly related to cancer cells of the same type. Here we report a comprehensive analysis of human rRNA Nm modification variability observed for >15 human cell lines grown in different media and conditions. Our data demonstrate that human Nm sites can be classified into four groups, depending on their observed variability. About 1/3 of rRNA 2'-O-methylations are invariably modified at the same level in all tested samples, the second relatively stable group of constitutive modifications (another ½ of the total) shows a slightly higher variance and two variable groups, showing an important heterogeneity. Mapping of these four classes on the human ribosome 3D structure shows that stable positions are preferentially located in the important ribosome functional sites, while variable and highly variable residues are mostly distributed to the ribosome periphery. Possible relationships of such stable and variable modifications to the ribosome functions are discussed.