PRJNA446060
Tracking the genetic evolution of daptomycin resistance in Staphylococcus aureus using an in vitro bioreactor model
We describe a novel approach to studying the accumulation of genetic mutations that lead to enhanced DAP-R in N315, a highly DAP-S strain of S. aureus. We employed a continuous culture model using a bioreactor which, eliminates the conditions of nutrient limitation and allows bacterial replication to occur unhindered except for antibiotic exposure.