GSE193556
Analysis of transcriptome response of rhizobia to aluminium toxicity
Purpose: High throughput sequencing has revolutionized methods of microbial pathway analysis in response to various envoronmental stimuli. The aim of this study was to determine mechanisms in which Rhizobium phaseoli employ towards tolerance or resistance to aluminium toxicity through RNA sequencing. Methods: Total RNA was extracted from bacteria treated with aluminium after 48 hours with trizol and sequenced with illumina's Miseq at Novogene Corporation Inc. U.K. After QC, reads were aligned to the genome with bowtie2 and differential expression performed with DESeq2. Results: Sequencing resulted to about 6.7-9.0 M reads that aligned to all the four plasmids and one chromosome of R. phaseoli genome. Most enriched genes were found localized in the membrane of the bacteria. Conclusion: The membrane of R. phaseoli is possibly the target site for toxicity and tolerance against aluminium toxicity. This is the first time such a study is described. Overall design: Total RNA was isolated from 0.1 mM aluminium treated and untreated cells. The experiment was repeated three times. Together, six samples were analysed.