GSE195469
Lariat mapping of DBR1 KO and DBR1 KO+DBR1 addback cells
We constructed a DBR1 knockout cell line (C22) using CRISPR in HEK293T cells. Through mapping of lariat reads, lariat levels in the DBR1 - samples are shown to increase dramatically (~20x) relative to wild type cells. Over 60% of this increase in lariat levels is abrogated upon rescue of DBR1 - cells with a DBR1 expression vector Overall design: Lariat mapping of wild type, DBR1 -, and DBR1 - +DBR1 addback HEK293T cells