PRJNA792598
Targeted sequencing of mouse pseudo-polar bodies
Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. In attempts to study the possibility of somatic genome haploidization, we replaced the meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle and triggered the extrusion of the pseudo-polar body (PPB). Here, we report targeted DNA amplicon sequencing of ten individual PPBs. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes.