ena-STUDY-CHUV-UNIL-19-08-2014-21:21:05:774-720
Genome sequencing and analysis of Estrella lausannensis by MLS master students of the University of Lausanne, Switzerland
This entry reports the results of Master students that specifically sequenced and annotation te genome of Estrella lausannensis CRIB 30, an obligate intracellular bacterium related to Chlamydia. Bacterial DNA was sequenced using Illumina GaIIx machine with half of a paired-end 36 bp run. The reads obtained were assembled using different softwares. A few gaps were solved by PCR, Sanger sequencing and reassembly. The genome was annotated with GenDB. Students focalized on the manual curation of the annotation of particular biosynthetic pathways to evaluate the ability of E. lausannensis to synthesize essential building blocks such as nucleotides, amino acids and co-factors. The complete sequence of the plasmid was solved and the gene content of the plasmid was given a particular attention as well.
This entry reports the results of Master students that specifically sequenced and annotation te genome of Estrella lausannensis CRIB 30, an obligate intracellular bacterium related to Chlamydia. Bacterial DNA was sequenced using Illumina GaIIx machine with half of a paired-end 36 bp run. The reads obtained were assembled using different softwares. A few gaps were solved by PCR, Sanger sequencing and reassembly. The genome was annotated with GenDB. Students focalized on the manual curation of the annotation of particular biosynthetic pathways to evaluate the ability of E. lausannensis to synthesize essential building blocks such as nucleotides, amino acids and co-factors. The complete sequence of the plasmid was solved and the gene content of the plasmid was given a particular attention as well.