GSE48703
GSE48703: Identification of Transcription Factor EFL-1::GFP Binding Regions in Ab
Summary: modENCODE_submission_3605 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals.  Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: DevStageWorm:L1 26dC:MS:1(official name : L1 26dC ); Developmental Stage: L1 larva; Genotype: wild type; Sex: Hermaphrodite;  EXPERIMENTAL FACTORS: Developmental Stage L1 larva; Target gene efl-1; Strain DevStageWorm:L1 26dC:MS:1(official name : L1 26dC ); temp (temperature) 20 degree celsius