pmoA_pyrosequencing_1
The potential of pmoA amplicon pyrosequencing for methanotroph diversity                 studies
We analyzed the potential of pmoA amplicon pyrosequencing compared to                 Sanger sequencing with paddy soils as model environment. We definded OTU cutoff                 values of 7 and 18 percent reflecting methanotrophic species and major phylogenetic                 pmoA lineages, respectively. Major lineages were already well covered by clone                 libraries, nevertheless, pyrosequencing provided higher diversity at the species                 level.