GSE125389
Anti-apoptotic regulation contributes successful nuclear reprogramming using cryopreserved oocytes
The cryopreservation has a negative effect on the quality of oocytes and may be  closely associated with increased levels of reactive oxygen species (ROS) and  apoptotic events. The purpose of the present study was to evaluate the detrimental  effects on the developmental competence of somatic cell nuclear transferred (SCNT)-  mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery  effects of melatonin on cryo-damage in cloned embryos. Development of SCNT  embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those  using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation  of 8 pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1,  P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin,  an agent that reduces apoptosis and ROS production, enhanced blastocyst formation  rates in the SCNT-CROP group when compared with the melatonin-untreated group.  Additionally, melatonin-treatment increased the derivation efficiency of pluripotent stem  cells from cloned embryos using cryopreserved oocyte. Overall design: Here we perform RNA-Seq experiment in melatonin-treated mouse 2-cell and blastocyst embryos, respectively.