PRJNA480312
16S rRNA Marker-gene survey Two-sample titration dataset
16S rRNA marker-gene survey assessment dataset generated using mixtures of human stool DNA extracts. We used samples collected from participants in an Enterotoxigenic Escherichia coli (ETEC) vaccine trial. A two-sample titration mixture design was used where DNA from stool samples collected prior to ETEC exposure were titrated into stools samples collected after exposure, in effect diluting the amount of ETEC in the mixed sample. Independent titration series were produced using samples from five trial participants. The titration series consisted of 9 samples including the unmixed samples. Four 16S PCR replicates were generated for each titration. The PCR products were sent to two independent laboratories for library preparation and sequencing. The libraries were sequenced twice at each laboratory resulting in four sequencing datasets.