GSE126997
Myc regulation of ETC Biogenesis and mtDNA Replication
To investigate the important role of Myc in regulating mitochondrial respiration and DNA biogenesis during Drosophila oogenesis, we dissected ovaries with developmental stages from germarium to stage 10 egg chambers from 4-6 day-old w1118 and mycP0/P0 mutant flies. We purified and compared mRNA transcriptome profiles (RNA-seq) in triplicate. We found that the mRNA levels for about 75% components in ETC, heme metabolism, mtDNA replication and translation pathways are decreased in mycP0/P0 mutant. Interestingly, about 85.7% down-regulated mitochondrial genes have predicted Myc binding sites (E-box: CACGTG) in their regulatory regions. Considering that not all E-box are active, it is possible that mitochondrial biogenesis is also indirectly controlled by Myc through a secondary transcriptional regulation. We found that Myc also mediate expression of 48 TFs, 35 of which with predicted E-box in their regulatory regions. Hence, our results indicated that Myc is a master regulator of ETC biogenesis through mtDNA replication and ETC gene expression. Overall design: Total mRNA were extracted from ovaries of w1118 and mycP0/P0 mutant flies. The mRNA profiles were generated in triplicate using Hiseq3000.