PRJNA215697
Rattus norvegicus Transcriptome or Gene expression
In this study, we initiated to detecte the whole-genome miRNAs expression profiles of E2 on both HSC-T6 and PS-induced fibrotic liver of rats by sequencing technology. in the PS-induced liver fibrosis models, five miRNAs were downregulated and four miRNAs were upregulated in PS-induced hepatic fibrosis versus normal liver. Furthermore, five miRNAs were significantly underexpressed and two miRNA were overexpressed with E2 treatment compared with without E2 treatment. While in HSC-T6 models, fifty miRNAs were significantly underexpressed and three miRNAs were overexpressed with E2 treatment compared with without E2 treatment. Among these miRNAs, there are five common miRNAs(miR-100, miR-138, miR-181b, miR-342-5p, miR-361) expressed and validated in hepatic fibrosis both in vivo and in vitro. Further function annotation of the miRNA targets revealed that the common miRNAs may play an important role in biological functions such as dorso-ventral axis formation, gap junction, focal adhesion, oncogenic signaling pathways and so on.