GSE169722
Long Non-coding RNA LINC02474  Affects Metastasis and Apoptosis  of Colorectal Cancer by Inhibiting  the Expression of GZMB
Background: Colorectal cancer (CRC) is one of the most frequently diagnosed  malignancies. Metastasis is the main event that impedes the therapeutic effect on CRC,  and its underlying mechanisms remain largely unclear. LINC02474 is a novel long  noncoding RNA (lncRNA) associated with metastasis of CRC, while little is known  about how LINC02474 regulates these malignant characteristics.  Methods: Expressions of LINC02474 and granzyme B (GZMB) were assessed by  quantitative real-time polymerase chain reaction (qRT-PCR) or Western blotting  analysis. Cell metastasis was detected by transwell assay and metastatic nude mouse  model, and apoptosis was determined by Western blotting analysis and ?ow cytometry.  Besides, the interaction between LINC02474 and GZMB was detected by dual-luciferase  reporter assays.  Results: The expression of LINC02474 was signi?cantly up-regulated in CRC tissues.  Moreover, depletion of LINC02474 damaged the metastatic abilities of CRC cells in vivo  and in vitro while boosting apoptosis. Besides, up-regulation of LINC02474 could  promote migration and invasion, while apoptosis was inhibited in CRC cells. Besides,  down-regulation of LINC02474 promoted the expression of GZMB, and interference of  GZMB could increase the metastatic abilities of CRC cells while reducing apoptosis.  Furthermore, LINC02474 was related to the transcriptional repression of GZMB in CRC  cells determined by the dual-luciferase reporter assay.  Conclusions: The ?ndings revealed that a novel lncRNA, LINC02474, as an oncogene,  could promote metastasis, but limit apoptosis partly by impeding GZMB expression in  CRC. Besides, LINC02474 had the potential to be used as a biomarker in the prognosis of  CRC. Overall design: there are 6 samples totally which have been grouped into 2 groups including three duplicates for each group. For group named "DLD-1-356", LINC02474 is knocked down stably, and for group named "DLD-1-NC", it is the control group.