ena-STUDY-UAMS-18-08-2017-00:12:57:498-47
We employed and combined the long reads sequencing technologies (MinION and PacBio) and short reads (Illumina). The de novo assembly of the reads derived from the three technologies was performed, resulted in the completion of the yeast genome.
Completion of eukaryotic genome is not trivial task because highly complexity of eukaryotic genome and limitations of sequencing technologies. Saccharomyces cerevisiae strain CEN.PK 1137D, which is widely used in academic and industry research, was recently sequenced and released its genome sequence with a good quality. However, discontinuity of individual chromosome and incompleteness still remain. Therefore, we employed and combined the long reads sequencing technologies (MinION and PacBio) and short reads (Illumina). The de novo assembly of the reads derived from the three technologies was performed, resulted in the completion of 16 chromosomes, mitochondrial chromosome and 2-micron plasmid in one step. The comparative genomics analyses between the reference strain S288c and CEN.PK 1137D identified key signature of genetic differences. Full-length transcripts were identified through direct RNA sequencing using MinION enabling highly accurate identification of transcriptional landscape in the CEN.PK 1137D strain.    This work demonstrated the potential strategy to obtain completion of genome sequencing that is able to apply on other eukaryotic organisms.
Completion of eukaryotic genome is not trivial task because highly complexity of eukaryotic genome and limitations of sequencing technologies. Saccharomyces cerevisiae strain CEN.PK 1137D, which is widely used in academic and industry research, was recently sequenced and released its genome sequence with a good quality. However, discontinuity of individual chromosome and incompleteness still remain. Therefore, we employed and combined the long reads sequencing technologies (MinION and PacBio) and short reads (Illumina). The de novo assembly of the reads derived from the three technologies was performed, resulted in the completion of 16 chromosomes, mitochondrial chromosome and 2-micron plasmid in one step. The comparative genomics analyses between the reference strain S288c and CEN.PK 1137D identified key signature of genetic differences. Full-length transcripts were identified through direct RNA sequencing using MinION enabling highly accurate identification of transcriptional landscape in the CEN.PK 1137D strain.    This work demonstrated the potential strategy to obtain completion of genome sequencing that is able to apply on other eukaryotic organisms.