GSE196220
mRNA transcriptome sequencing of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice (Axin1KO?IEC) that have udergone DSS-induced colitis and AOM/DSS-induced colorectal tumorigenesis
Purpose : The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of colon samples of intestinal epithelial cell specific Axin1 Knockout mice and WT controls that were submitted to DSS-induced colitis and AOM/DSS-induced colorectal carcinogenesis. Methods : DSS-induced colitis was performed on Axin1flfl (WT) and Vil CreERT2;Axin1fl/fl (Axin1KO?IEC) mice by giving 3% DSS dissolved in drinking water for 7 days and subsequently placed on regular water for recovery before sacrifice at Day 7 and D13. Methods : AOM/DSS-induced colorectal tumorigenesis was performed on Axin1flfl (WT) and Vil CreERT2;Axin1fl/fl (Axin1KO?IEC) mice  that were sacrificed at day 100 post-AOM injection to collect colorectal tumors. Methods : Colonic mRNA profiles of WT and Axin1KO?IEC mice were generated by deep sequencing using Illumina NextSeq 500 instrument (150base-lengths read V2 chemistry in a paired-end mode) Overall design: Colonic mRNA profiles of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice that have udergone DSS-induced colitis. Three biological replicates for each group.Colorectal tumor mRNA profiles of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice that have undergone AOM/DSS-induced colorectal tumorigenesis. Three biological replicates for each group