CRISPR_gRNA_library_validation-sc-3081
CRISPR_gRNA_library_validation
PCR products were generated using genome-wide CRISPR libraries as template DNA. Subsequently, PCR products are pooled and subjected to Illumina library preparation.  The library will be sequenced by HiSeq2500 RapidRun.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ submitted for publication. For information on the proper use of data shared by the Wellcome Trust Sanger Institute (including information on acknowledgement), please see http://www.sanger.ac.uk/datasharing/
The CRISPR-Cas system is a new DNA-editing technology and promise potential in many applications such as genome engineering and genetic screening. We are generating genome-wide CRISPR guide RNA libraries using pooled oligos that are synthesized on microarray slides. We want to analyse the quality of the resulting libraries by deep sequencing.