GSE172349
Polysome Profiling in Isoleucine tRNA Isoswitch Cells
Polysome Profiling was used to evaluated whether changes in abundance of tRNAIleUAU and tRNAIleGAU would affect codon dependent translation and quantify the abundance of these tRNAs in highly translated fractions. Overall design: Examination of highly translated transcripts under Isoleucine tRNA modulation (increased tRNAIleUAU expression and decreased tRNAIleGAU expression via CRISPRi)