DRP006509
Identification of mutations conferring resistance to 10 anti-tuberculosis drugs by laboratory evolution of non-pathogenic Mycobacterium on agar plate.
To identify mutations conferring resistance to anti-TB drugs, laboratory evolution of non-pathogenic M. smegmatis which is closely related to M. tuberculosis were conducted. For each obtained drug-resistant strain, whole-genome sequencing analysis using Illumina Miseq identified new resistance-conferring mutations.