E-MTAB-8497
Sequencing of RNAs derived from the purified endoplasmic reticulum of human LP1 myeloma cells expressing either the wt FAM46C protein or the D90G mutant allele
Endoplasmic reticula (ER) were isolated from LP1 human myeloma cells expressing either the wt FAM46C protein or the D90G mutant version, which is often found in myeloma patients. RNAs were purified from the ER and sequenced. Quantitation and characterization of specifically ER-bound mRNAs is important in determining the secretomic potential of cells. In this case we focused on comparing cells expressing the two forms of the FAM46C protein in the LP1 cell line which lacks FAM46C expression.
Endoplasmic reticula (ER) were isolated from LP1 human myeloma cells expressing either the wt FAM46C protein or the D90G mutant version, which is often found in myeloma patients. RNAs were purified from the ER and sequenced. Quantitation and characterization of specifically ER-bound mRNAs is important in determining the secretomic potential of cells. In this case we focused on comparing cells expressing the two forms of the FAM46C protein in the LP1 cell line which lacks FAM46C expression.