PRJNA552827
Suppression mutation of  LTA deficient Staphylococcus aureus
This study aims to identify suppression mutations in LTA deficient Staphylococcus aureus. LTA deficient Staphylococcus aureus was made by deletion of gene ltaA in strain NCTC8325. The ltaA deletion strain shows strong growth defect on Luria-Bertani (LB) agar plate low pH (pH=6.0), and colonies with restored growth show up sporadically. Whole genome sequencing of 3 colonies with restored growth and the parent ltaA deletion strain identifies suppressor mutations in each of the 3 colonies.