GSE113491
Small RNA seq in epididymis of wild type and Alkbh3-/- mice
Total RNA (100 µg) were denatured at 70 °C for 5 min and separated by a 15% TBE-Urea gel with 20/100 or 10/60 oligo length standard ladder (Integrated DNA Technologies, Coralville, IA). After visualized by SYBR Gold (Ref. S11494 from Life Technologies), tRFs in gels were cut according to sizes and recovered by small RNA PAGE recovery kit (Zymo Research, Irvine, CA, USA). The recovered tRFs was used to establish the library by NEB small RNA library preparation kit (E7330S). Overall design: Examination of small RNA difference in epididymis between wild type and Alkbh3-/- mice