GSE130143
FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
FIN-Seq is a method that allows transcriptional profiling of specific cell types in frozen postmortem human CNS tissues. Briefly, nuclei from frozen CNS samples are isolated, fixed, and immunolabeled with antibodies for prospective isolation. The RNA from the FACS isolated nuclei are extracted and an RNA sequencing library is generated for subsequent sequencing. Overall design: Isolation of two cortical neuronal subtypes in mice: Corticofugal projection neurons and Callosal projection neurons using a BCL11B antibody and SATB2 antibody, respectively. Also, a comparison between cellular RNA and nuclear RNA from corticofugal neurons at P7.