ena-STUDY-STATION BIOLOGIQUE DE ROSCOFF-02-04-2020-14:55:59:387-7
Effect of swab, silica-gel and shock-freezing on the microbiota of the brown alga Saccharina latissima.
In this study we evaluated the impact of swab, silica-gel or shock-freezing in liquid nitrogen on the Saccharina microbiota. We performed 16S Metabarcoding of the V3-V4 region with the standard Illumina protocol for metabarcoding (Q5® High-Fidelity PCR Kit, AMPure XP for PCR Purification Kit and Nextera XT DNA Library Preparation Kit), and the sequences were obtained with an Illumina MiSeq sequencer using a MiSeq v3 kit (2x300bp, paired-end).
In this study we evaluated the impact of swab, silica-gel or shock-freezing in liquid nitrogen on the Saccharina microbiota. We performed 16S Metabarcoding of the V3-V4 region with the standard Illumina protocol for metabarcoding (Q5® High-Fidelity PCR Kit, AMPure XP for PCR Purification Kit and Nextera XT DNA Library Preparation Kit), and the sequences were obtained with an Illumina MiSeq sequencer using a MiSeq v3 kit (2x300bp, paired-end).