GSE106427
BATF regulates the expression of Nfil3, Wnt10a and miR155hg for efficient induction of antibody class switch recombination
BATF functions in both T cells and B cells to control the host response to antigen and promote the production of class switched immunoglobulins.  In this study, we demonstrate that BATF expression is increased rapidly, and transiently, immediately following B cell stimulation and use an inducible model of BATF deletion to show that this induction is necessary, and sufficient, to trigger the program of  immunoglobulin class switch recombination (CSR).   We identify two genes (Nfil3 and miR155gh) that are positively regulated and one gene (Wnt10a) that is negatively regulated by BATF during CSR.   All three of these BATF targets play an essential role in CSR and each impacts the expression and/or function of the others.  Our observations allow for the positioning of these targets in a network downstream of BATF induction and upstream of the transcriptional activation of Aicda – the gene encoding the enzyme responsible for immunoglobulin gene rearrangements.   This work extends the knowledge of the molecular control of CSR and, importantly, positions the induction and function of BATF as an early event in this process. Overall design: B cells were isolated from 4WT and 3Batf KO mouse spleens using MACS columns.  B cells were activated for 6hrs with LPS and IL-4.  Total RNA was harvested for for RNA-seq. All Samples have 2 replicates