GSE135614
Identification of known and novel miRNAs in the apical and basal spikelets of rice Oryza sativa cv. Mahalaxmi
The advent of next generation sequencing (NGS) has greatly enriched the database of miRNAs. For plants so far 8455 miRNAs sequences from 73 species and 15401 miRNAs sequences from 150 species have been deposited in miRBase 22.0 and Plant Non-coding RNA Database, respectively. The occurrence of miRNAs in such a huge number, which is still increasing, is because of the fact that the profile of miRNAs expression differs greatly from species to species, both quantitatively and qualitatively. Besides, even within a species it is expected that the miRNA expression profile would differ from cultivar to cultivar depending on the trait with regard to which the two cultivars differ. Beises, the expression of a miRNA in a plant organ may differ depending upon it spatial location. The same is reflected in the NGS data of the apical and basal spikelets of the panicle of the rice cultivar Mahalaxmi. Overall design: Spikelets of Oryza sativa cv. Mahalaxmi  were  sampled on the 6th days after fertilization from the apical and basal region.  sRNAs were isolated from the apical and basal spikelets. Known and novel miRnAs were identified bioinformatically, and their expression was validated by Northern.