PRJNA532991
Mus musculus strain:Sv129 | breed:Inbreed Raw sequence reads
In this study, we firstly investigated the effect of genetic background for isolating of AFSCs from chemical defined medium. We found that AFSCs could be generated from blastocysts of pure Sv129 strain mice, ICR strain mice and hybrid mice; except pure C57BL/6 and non-obese diabetic (NOD). In the second, using Act A and BMP4, the AFSCs were able to convert to trophoblast stem cells like stem cells (TSLSCs) which contributed to both ICM and trophectoderm (TE), as well as extraembryonic tissues in chimera. Due to the culture conditions applied to derive EpiSCs were essentially the same as those used for propagating human pluripotent stem cells, our findings provide new insights into the study of differentiation of human embryo stem cells.