Marine_Phage_Sequencing_Metagenomics_Pacific_Ocean_Sediment
4 samples from Pacific Ocean uncultured phage metagenome
Anaerobic oil degrading sediment metagenome - anaerobic subsurface sediments containing Monterey Formation oil and hydrocarbon gasses; sample description: Shane Seep; Coal Oil Point hydrocarbon seep field, offshore Goleta, CA.  0.22 micron filtered; CsCl-gradient; DNAse treatment.        ANME metagenome - carbonate mound formed by hydrate crystalization and expansion;  actively releasing methane from sediments; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment, amplified via MDA with phi29 polymerase.       Methanogenic sediments metagenome - anoxic sediments from center of Santa Barbara Basin. Basin water exhibits suboxic conditions at sampling depth.  Subsurface environment detailed in refs 1&2.  Methanogenesis confirmed through intermittent incubation headspace analyses; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment.       Benthic methanotrophic mats metagenome - sample cultivated ~1 month above active hydrocarbon gas vent; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment.