PRJNA386950
semi-deserted soil layers Raw sequence reads
The sampling sites (84o42'E, 45o36'N) located in the northwest of Dzungharian Basin, northwest of China (Fig. 1A). It is a largely a semi-desert area with the crude oil mining begun on 1950s. The samples were taken in the middle of October 2014. Eight sampling sites dispersed around the whole oilfield mainly closed to the oil-wells were taken by augers and cores, and were divided into different layers as 5-15 cm,25-35 cm,45-55 cm.Total DNA extraction from the petroleum contaminated soil samples. The 16S rRNA gene V4 region was amplified by using barcoded specific primer set 515F (5’-GTGCCAGCMGCCGCGGTAA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’) . PCR products from different soil layers were then gone library construction and sequencing. Sequencing libraries were generated using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer’s recommendations, and then the libraries were sequenced on an IlluminaHiSeq 2500 platform.