GSE67121
Transcriptome Characterization of developing bean (Phaseolus vulgaris L.) pods from two genotypes with contrasting seed zinc concentrations.
Dry bean (Phaseolus vulgaris L.) seeds are a rich source of dietary zinc, especially for people consuming plant-based diets.  Within P. vulgaris there is at least two-fold variation in seed Zn concentration.  Genetic studies have revealed seed Zn differences to be controlled by a single gene in two closely related navy bean genotypes, Albion and Voyager.  In this study, these two genotypes were grown under controlled fertilization conditions and the Zn concentration of various plant parts were determined.  The two genotypes had similar levels of Zn in their leaves and pods but Voyager had 52% more Zn in its seeds than Albion. RNA was sequence from developing pods of both genotypes.  Transcriptome analysis of these genotypes identified 27,198 genes in the developing bean pods, representing 86% of the genes in the P. vulgaris genome (v 1.0 DOE-JGI and USDA-NIFA).  Expression was detected in 18,438 genes.  A relatively small number of genes (381) were differentially expressed between Albion and Voyager.  Differentially expressed genes included three genes potentially involved in Zn transport, including zinc-regulated transporter, iron regulated transporter like (ZIP), zinc-induced facilitator (ZIF) and heavy metal associated (HMA) family genes.  In addition 12,118 SNPs were identified between the two genotypes.  Of the gene families related to Zn and/or Fe transport, eleven genes were found to contain SNPs between Albion and Voyager. Overall design: Transcriptome of developing pods of two contrasting common bean genotypes in zinc concentration  generated by deep sequencing using Illumina Genome Analyzer II