GSE126743
Genome-wide maps of chromatin state in TRIB3-shRNA and control lymphoma cells
We report the application of single-molecule-based sequencing technology for high-throughput profiling of  RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in TRIB3-cas9 and control group. Inspection of multiple individual traces and global analyses showed that TRIB3 deletion globally reduced association of RNAPII with promoters. Overall design: we performed ChIP sequencing with antibody to total RNAPII in TRIB3 deleted group. Inspection of individual traces and global analyses showed that TRIB3 deletion globally reduced association of RNAPII with promoters.