ena-STUDY-YALE SCHOOL OF MEDICINE-25-06-2019-16:50:00:663-135
Disentangling diet-host-microbiota interactions with a defined human gut bacterial community
To disentangle diet, host and microbiome contributions to the host metabolic phenotype in a controlled system, we assembled a synthetic consortium of 18 representative, genome-sequenced human gut microbes, and compared diet response in gnotobiotic mice carrying this defined community against germfree mice. We analyzed gut community adaptations to high-fat versus high-carbohydrate diets with metagenomics and metatranscriptomics sequencing of mouse cecal samples.  Initial community composition:  Bacteroides thetaiotaomicron VPI-5482 tdk53 Bacteroides caccae ATCC 43185 Bacteroides ovatus ATCC 8483 Bacteroides uniformis ATCC 8492 Phocaeicola vulgatus ATCC 8482 (formerly Bacteroides vulgatus) Parabacteroides distasonis ATCC 8503 Bacteroides stercoris ATCC 43183 Blautia hydrogenotrophica ATCC BAA-2371 Blautia obeum ATCC 29174 Clostridium scindens ATCC 35704 Clostridium symbiosum ATCC 14940 Escherichia coli BW25113 Eubacterium rectale ATCC 33656 Ruminococcus torques ATCC 27756 Marvinbryantia formatexigens DSM14469 Collinsella aerofaciens ATCC 25986 Bifidobacterium adolescentis ATCC 15703 Bifidobacterium longum CCUG52486.  High-fat diet: TD.06414, Envigo Teklad Diets High-carbohydrate diet: TD.140806, Envigo Teklad Diets
To disentangle diet, host and microbiome contributions to the host metabolic phenotype in a controlled system, we assembled a synthetic consortium of 18 representative, genome-sequenced human gut microbes, and compared diet response in gnotobiotic mice carrying this defined community against germfree mice. We analyzed gut community adaptations to high-fat versus high-carbohydrate diets with metagenomics and metatranscriptomics sequencing of mouse cecal samples.  Initial community composition:  Bacteroides thetaiotaomicron VPI-5482 tdk53 Bacteroides caccae ATCC 43185 Bacteroides ovatus ATCC 8483 Bacteroides uniformis ATCC 8492 Phocaeicola vulgatus ATCC 8482 (formerly Bacteroides vulgatus) Parabacteroides distasonis ATCC 8503 Bacteroides stercoris ATCC 43183 Blautia hydrogenotrophica ATCC BAA-2371 Blautia obeum ATCC 29174 Clostridium scindens ATCC 35704 Clostridium symbiosum ATCC 14940 Escherichia coli BW25113 Eubacterium rectale ATCC 33656 Ruminococcus torques ATCC 27756 Marvinbryantia formatexigens DSM14469 Collinsella aerofaciens ATCC 25986 Bifidobacterium adolescentis ATCC 15703 Bifidobacterium longum CCUG52486.  High-fat diet: TD.06414, Envigo Teklad Diets High-carbohydrate diet: TD.140806, Envigo Teklad Diets