ena-STUDY-JULIUS KUHN-INSTITUT (JKI), FEDERAL RESEARCH CENTRE FOR CULTIVATED PLANTS-04-11-2020-12:26:15:260-177
Genotyping by sequencing of caraway (Carum carvi)
137 caraway accessions of various origin were genotyped using genotyping by sequencing (GBS, Elshire et al. 2011). In detail, 100 ng of genomic DNA and 1.44 ng of total adapters were used; the genomic DNAs were restricted with EcoT22i enzyme; the libraries were amplified with 18 PCR cycles. The samples were split up into two libraries. A paired end sequencing was carried out using ILLUMINA HiSeq X Ten.For each accession, two separate pools of five individual plants were genotyped. This resulted in 274 samples.
137 caraway accessions of various origin were genotyped using genotyping by sequencing (GBS, Elshire et al. 2011). In detail, 100 ng of genomic DNA and 1.44 ng of total adapters were used; the genomic DNAs were restricted with EcoT22i enzyme; the libraries were amplified with 18 PCR cycles. The samples were split up into two libraries. A paired end sequencing was carried out using ILLUMINA HiSeq X Ten.For each accession, two separate pools of five individual plants were genotyped. This resulted in 274 samples.