ena-STUDY-THE ROCKEFELLER UNIVERSITY-07-02-2019-22:11:43:943-99
Differential gene analysis to identify genes expressed in CEPsh glia of C. elegans
CEPsh glia were isolated from C. elegans mix larvae population by FACS, based on expression of a GFP marker under control of the CEPsh glia-specific marker hlh-17. All other cells, which are not expressing GFP, were collected separately for control. RNA reads from GFP-positive and GFP-negative samples were compared to identify CEPsh glia-enriched genes.
CEPsh glia were isolated from C. elegans mix larvae population by FACS, based on expression of a GFP marker under control of the CEPsh glia-specific marker hlh-17. All other cells, which are not expressing GFP, were collected separately for control. RNA reads from GFP-positive and GFP-negative samples were compared to identify CEPsh glia-enriched genes.