GSE133204
Single-cell transcriptomic analysis of 96hour AEL wild type wing imaginal discs
Genotype: FRT82B Method: Staged wild type wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ? for 10 min. Cells were then washed in DPBS and passed through 35µm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene. Overall design: 10X  based single-cell mRNA profiles of wild type wing imaginal discs