GSE183070
Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and long noncodingRNAs with putative functional significance
Long noncoding (lnc)RNA modulate gene expression alongside presenting unexpected source of neoantigens. Despite their immense interest, little is known in their ability to be transferred and control adjacent cells. Extracellular Vesicles (EVs) offer a protective environment for nucleic acids, with pro and anti-tumorigenic functions by controlling the immune response. However, few studies have addressed the full RNA content of EVs in human fluids and none have compared them with their tissue of origin. Here, we performed Total RNA-Sequencing on 6 Formaldehyde-Fixed-Parafilm-Embedded (FFPE) prostate cancer (PCa) tumor tissues and their paired urinary (u)EVs to provide the first whole transcriptome comparison from the same patients. uEVs contain intron-free cytoplasmic transcripts, specific lnc/circular (circ)RNAs. Some uEV-circRNA were characterized as essential for prostate cell proliferation in vitro. 15 uEV-lncRNA were predicted encoding 750 unique high-affinity neoantigens. Our comparison of functional EVs-lnc/circRNAs in 3 PCa cell lines provides a fundamental resource to determine the role of uEV-lncRNA in PCa progression. Overall design: Here, we performed Total RNA-Sequencing on 6 Formaldehyde-Fixed-Parafilm-Embedded (FFPE) prostate cancer (PCa) tumor tissues and their paired urinary (u)EVs to provide the first whole transcriptome comparison from the same patients. Total RNA was extracted, checked for the quality and then converted into cDNA for total stranded RNA-sequencing.