GSE174733
Protection against autoimmunity is driven by thymic epithelial cell-mediated regulation of Treg development
Thymic education of Tregs is superior to negative selection in the prevention of autoimmunity. To address this hypothesis, TCR repertoirs of thymic and peripheral Tcells (including Tregs) from conditional NIK KO and WT mice have been sequenced and compared. Overall design: conventional CD8, CD4 T cells and Tregs cells have been sorted from thymus, spleen and lung of 3 WT and 3 Foxn1CrexNIKflfl mice. The analysis is based on the sequecing of the respective TCRb RNAs