ena-STUDY-CHALMERS UNIVERSITY OF TECHNOLOGY-07-12-2018-15:46:31:177-218
RNAseq analysis of STB5 overexpression in Saccharomyces cerevisiae
The endogenous transcription factor STB5 was overexpressed in the yeast Saccharomyces cerevisiae by replacing its native promoter with the promoter of the endogenous gene TPI1. The control and the overexpression strain were cultivated in bioreactors in quadruplicates with 2% glucose, and samples for RNAseq analysis were taken in exponential phase (glucose) and after strains resumed growth after the diauxic shift (ethanol). Continuous, glucose-limited, cultivation was then initiated (7.5 g/L glucose in the feed, dilution rate of 0.1 h-1), and after strains were confirmed to have reached steady state they were once again sampled for RNAseq analysis.
The endogenous transcription factor STB5 was overexpressed in the yeast Saccharomyces cerevisiae by replacing its native promoter with the promoter of the endogenous gene TPI1. The control and the overexpression strain were cultivated in bioreactors in quadruplicates with 2% glucose, and samples for RNAseq analysis were taken in exponential phase (glucose) and after strains resumed growth after the diauxic shift (ethanol). Continuous, glucose-limited, cultivation was then initiated (7.5 g/L glucose in the feed, dilution rate of 0.1 h-1), and after strains were confirmed to have reached steady state they were once again sampled for RNAseq analysis.