GSE163232
SUM159 cells treated with H3B-8800 or DMSO, Cytoplasmic fraction RNA-seq
The objective is to identify splicing changes that occur after spliceosome inhibition in triple-negative breast cancer, specifically in cytoplasmic RNA. Overall design: SUM159 cells treated with H3B-8800 (spliceosome inhibitor) or vehicle (DMSO) for 12 hours in biological duplicate. RNA was isolated from the cytoplasm for downstream processing.