GSE108279
Evaluation of mRNA expression in the equine chorioallantoic membrane
Purpose:The goal of this study was to evalute gene expression patterns of equine chorioallantoic membrane during different stages of the pregnancy Method: mRNA profile of equine chorioallantoic membrane (CAM) from 45days, 4months, 6months and 10months  (4 samples for each time points) generated by RNA-sequencing,using a Illumina HiSeq 4000 ( HiSeq 4000 sequencing kit version 1). The sequence reads  were trimmed for adapters and quality using TrimGalore Version 0.4.4,and then mapped to EquCab2.0 using STAR-2.5.2b.  Final quantification at the gen level was performed by analyzing the BAM files in cufflinks using the Equus_caballus_ENSEMBL_88 gtf file as Guide. Overall design: mRNA profile of equine chorioallantoic membrane (CAM) generated by RNA-sequencing, using a Illumina HiSeq 4000 (HiSeq 4000 sequencing kit version 1).