GSE129537
Exploring Listeria monocytogenes transcriptomes in correlation with divergence of lineages and virulence measured in Galleria mellonella
For bacteria in general and Listeria monocytogenes in particular, little is known about transcriptome diversity in comparison to the copious data on intraspecific genome diversity. The current study employed RNA sequencing to investigate the variation of transcript levels of conserved single copy genes from several perspectives including differences between lineages and correlation with previous classification of the virulence potential of clonal complexes based on epidemiological MLST data. Infection of G. mellonella, a surrogate host, also allowed a correlation analysis with virulence data experimentally determined in vivo. Overall design: The study encompasses 66 samples corresponding to 2 biological replicates (independent cultures) for 33 Listeria monocytogenes isolates belonging to the two major phylogenetic lineages, grown in an optimal in vitro culture condition.