ena-STUDY-CBMSO-06-11-2017-13:36:27:181-100
Genome sequence and transcriptome for the HU3 strain of Leishmania donovani. For genomic DNA sequencing, two NGS methodologies were used: PacBio and Illumina. On the other hand, to define the transcriptome, total poly-A+ RNA was sequenced using Illumina HiSeq 2000 technology.
This project was aimed to determine the genome sequence and transcriptome for the HU3 strain of Leishmania donovani. For genomic DNA sequencing, two NGS methodologies were used: PacBio and Illumina. These sequence reads were used to assemble the complete genome, composed by 36 chromosomes per haploid set. Afterwards, gene annotation (CDS for protein coding-genes and structural RNAs) was carried out by a combination of automated procedures and manual revision. On the other hand, to define the transcriptome, total poly-A+ RNA was sequenced using Illumina HiSeq 2000 technology. After transcript assembly, the boundaries of transcripts were determined by mapping spliced-leader (5'-end) and poly-A (3'-end) addition sites. As a result, in this project, we are providing the following data:- PacBio and Illumina sequence reads from genomic DNA.- Illumina reads from polyA+ RNA (RNAseq)- Nucleotide sequence of the 36 chromosomes.- Annotation data for transcripts, CDS, structural RNAs (rRNAs, tRNAs and snoRNAs).
This project was aimed to determine the genome sequence and transcriptome for the HU3 strain of Leishmania donovani. For genomic DNA sequencing, two NGS methodologies were used: PacBio and Illumina. These sequence reads were used to assemble the complete genome, composed by 36 chromosomes per haploid set. Afterwards, gene annotation (CDS for protein coding-genes and structural RNAs) was carried out by a combination of automated procedures and manual revision. On the other hand, to define the transcriptome, total poly-A+ RNA was sequenced using Illumina HiSeq 2000 technology. After transcript assembly, the boundaries of transcripts were determined by mapping spliced-leader (5'-end) and poly-A (3'-end) addition sites. As a result, in this project, we are providing the following data:- PacBio and Illumina sequence reads from genomic DNA.- Illumina reads from polyA+ RNA (RNAseq)- Nucleotide sequence of the 36 chromosomes.- Annotation data for transcripts, CDS, structural RNAs (rRNAs, tRNAs and snoRNAs).