Effect_of_WGA_lysis_buffers_on_substitution_profiles-sc-4567
Effect_of_WGA_lysis_buffers_on_substitution_profiles
Whole genome amplification of DNA from ESS1 using a variety of lysis conditions.
ESS1 has a specific mutational profile (signature 10) that can be used to distinguish somatic mutations from MDA artefacts.  Signature 10 is defined by C>T mutations at TCG and C>A mutations at TCT. The effect of different lysis and amplification methods on erorr profiles during MDA.