0531afec-c9e7-40c5-b319-0fc51259369f
Gene edition in haematopoietic cell lines
Due to the difficult transfection of blood cells, we developed an alternative strategy to generate CRISPR constructs. Our constructs were easily introduced into hard-to-transfect leukemic cells. Gene edition efficiencies obtained by Endonuclease T7-EI and restriction fragment length polymorphism were confirmed by sanger sequencing and next generation sequencing.
Due to the difficult transfection of blood cells, we developed an alternative strategy to generate CRISPR constructs. Our constructs were easily introduced into hard-to-transfect leukemic cells. Gene edition efficiencies obtained by Endonuclease T7-EI and restriction fragment length polymorphism were confirmed by sanger sequencing and next generation sequencing.