GSE107284
Transcriptomic profiling of ex vivo isolated  CD3+CD4+ T cells from spleens of colitic Rag-/- mice received naïve ERafl/fl and CD4CreERafl/fl CD4+ T cells
To study the role of estrogen receptor alpha (ERa) in T cell mediated inflammation, colitis was induced in Rag-/- mice by injecting FACS sorted CD4 T cells from control (ERafl/fl) and ERa deficient (CD4CreERafl/fl). After 8 weeks of injection, the CD4+ T cells were FACS sorted from Rag-/- spleens. RNA extraction was carried out and libraries were prepared using Illumina TruSeq RNA sample Preparation kit according to the manufacturer's instructions. Overall design: Transcriptomic profiling of ex vivo isolated  CD3+CD4+ T cells from spleens of colitic Rag-/- mice received naïve ERafl/fl and CD4CreERafl/fl CD4+ T cells