GSE104219
Gene expression program directed by an artificially evolved POU (ePOU)
We generated Oct4 libraries by randomizing selected amino acids and by recombining domains of paralogous POU family genes. These libraries were subjected to iterative rounds of pooled screens to select variants that enhance pluripotency induction. We identified an artificially evolved POU factor (ePOU) that substantially outperforms wild-type Oct4 in terms of mouse embryonic fibrobast (MEF) reprogramming. We compared the transcriptomes of cells reprogramming under ePOU or Oct4 conditions at day 3 using RNA sequencing. Overall design: mRNA profiles of 24 different reprogramming intermediate cells with ePOU/Oct4 in combination with Sox2/Sox17EK along with Klf4 and c-Myc, at day 0,2,3,4,6,and 13 of retrovirus system based