GSE196414
Global gene expression profile of cortices from Aifm1 KI and Hq mutant and control mouse
In this study, global transcriptome profiling was performed for Aifm1-KI and Hq mutant (as model of mitochondial defect) and control samples from mouse cortices, More than 20 million high-quality reads were obtained from each sample using Illumina platform. A consensus reference-guided assembly was generated for the transcriptome data from all samples and gene expression was analysed. Overall design: Total RNA was extracted from mutant and control samples and cDNA libraries were prepared. Sequencing was performed on Illumina HiSeq 4000 platform to generate 51 bp single-end reads. Pre-processing was performed to remove adapters and low-quality reads. Filtered high-quality reads were mapped on the mouse genome (mm10) using STAR aligner. To analyze gene expression, Htseq-count were used to quantify genes expression and EdgeR were used for differential expression.