DRP000033
Massive transcriptional start site mapping of mouse embryo 11days            cells.
Although recent studies have revealed that the majority of mouse genes            are subjected to regulation of alternative promoters (APs), the biological relevance of            this phenomenon remains unclear. To enable more comprehensive TSS analysis in the            respective cell types, we recently developed a method,combining oligo-capping with the            massively paralleled sequencing technology,Illumina GA. In this method, which we named            TSS Seq, sequence adaptor which is necessary for Illumina GA sequencing is directly            introduced to the cap site of the mRNA. By sequencing 36-48 sequence immediately            downstream of the TSSs (TSS tags), it is possible to obtain precise positional            information of transcriptional start sites (TSSs).
Comprehensive identification and characterization of the transcriptional            start sites of human genes were carried out. For this purpose, we used our TSS-Seq            method, in which next gene sequencing technology and our full-length cDNA library            technology, oligo-capping were combined.