GSE159737
Gene expression profiling of mouse MPN cells with or without STK11 knockout
We performed RNA-sequencing of mouse cells derived from colony forming assays (CFA) to evaluate the transcriptome of MPN cells with deletion of the tumor suppressor STK11/LKB1 and relative controls. The CFA are from mouse primary floxed STK11 hematopoietic stem and progenitor cells (HSPCs) transduced with retroviruses encoding the MPN mutation MPLW515L and CRE recombinase to delete STK11. Overall design: RNA sequencing differential expression analysis of hematopoietic cells from colony formation assays in triplicate: WT cells at plating 1, MPLW515L overxpressing cells at plating 1, MPLW515L STK11 -/- cells at plating 1,3,6