PRJNA429090
Comparison of intestinal microflora structure in UC patients by three sampling methods
In the present study,we collected 42 samples of 4 UC patients through biopsy forceps (BF), disposable specimen brush (DSB) and protected specimen brush (PSB). Using Illumina HiSeq platform, the V4 regions of bacterial 16S rRNA gene were amplified and sequenced. We observed that relative abundance and community structure of the biopsies differed significantly from that of the brush samples, showing clear separations of the three sampling methods, while the microbial pattern of the DSB and PSB were more similar. In addition, the differences were mostly caused by the relative abundance of microbial communities, rather than the influence of specific microbial species.