GSE132408
RNA-Seq in control and TET2 Knockout cells with IFN? treatment
Detect overall RNA level change under TET2 knockout in response to IFN?. Loss of TET2 alters IFN? transcriptome, TET2 mediates the IFN? signaling pathway. Overall design: The experiment is designed to determine the overall RNA level change between TET2 knockout and control group in response to IFN?. Briefly, we first generated TET2 KO THP1 cell lines by CRISPR technology. Then we treated control and TET2 KO THP1 cells with 100ng/ml IFN? for 20hrs, total RNAs from each group were extracted and sequenced, 3 replicates in each group and 2 technical repeat for each sample.