CRISPR_Cas9_mediated_DUB_knockout_screen_in_DDR-sc-4682
CRISPR_Cas9_mediated_DUB_knockout_screen_in_DDR
CRISPR/Cas9 screen for deubiquitinating enzymes (DUBs), E2 ubiquitin conjugating enzymes and components of the NEDD8 pathway, which are enriched or drop out  following exosure to different DNA damaging agents.
A focused  gRNA library targetting mouse of deubiquitinating enzymes (DUBs), E2 ubiquitin conjugating enzymes and components of the NEDD8 pathway was used in mouse ES cells exposed to IR, 6-TG and PARP inhibitor, Olaparib at low dose for drop-outs and high dose for resistance.