GSE135003
Transcriptome Analysis of TubA Effects on Oocytes Development in Mice
TubA is a specific inhibitor of HDAC6, it could regulate oocytes maturation by regulating acetylation of a-tubulin. However, weather it effects maternal mRNA expression remains unkown. Therefore, in this study,we got in vitro maturated MII oocytes in control and 0.1µg/mL treated .Our study suggests that TubA mainly downregulated cell cycle and DNA methylation related genes. Overall design: single cell mRNA profiles of control and TubA treated MII oocytes were generated by deep sequencing, 5 samples for each group, using Illumina HiSeq 2000