GSE185561
mRNA-sequencing data of Jurkat T cells with or without calcineurin inhibitors
In order to determine the calcineurin inhibitory effect of CABIN1 peptide, we performed RNA-sequencing in Jurkat T cells expressing negative contorl (HA-mCherry) or HA-mCherry-CABIN1 peptide. Jurkat T cells were activated by treatment 40 nM PMA and 1 µM Ionomycin for 8 hr. 0.5 µM FK506 (Tacrolimus, Tac) was pretreated for 1 hr before treatment with PMA and Ionomycin. Total RNA was extracted from these cells. Extracted RNA was used to prepare an mRNA sequencing library using TruSeq Stranded mRNA sample preparation kit. All samples were sequenced on Illumina NextSeq 500 with a 75 bp paired end read. Overall design: mRNA profiles of negative control and CABIN1 peptide expressing Jurkat T cells under the treatment with PMA and Ionomycin