CRISPR_SAM_screens-sc-3949
CRISPR_SAM_screens
CRSPR SAM libraries will be used in cancer cell lines to identify resistance mechanisms to anti-cancer drugs. Cancer cell lines will be given a whole-genome libraries, in order to create a frmaeshifts in the cell population. After cells will be exposed to anti-cancer drugs and resistant cells will be collected for DNA extractions and future sequencing. Amplicons containing gRNA sequences will be amplified , librarie will be created with different tags for Illumina sequencing.
Overexpression of genes is a quite common event during cancer progression and is a one of ther reason for anti cancer drug resistance. Here we are going to use activation CRISPR SAM library in order to overexpress whole genome, 1 gene per cell. Like this we will generate a population of cells  with 1 amplified gene per cell. The treatment with different anti-cancer drugs will kill the majrity of cells, and only thouse that have  drug resistnt overexpression will survive.