tatroviride
Identification of mycoparasitism-related genes in Trichoderma atroviride: A holistic comparative study on self confrontation and host interaction by de novo transcriptome sequencing
A high-throughput sequencing approach was utilized to carry out a   comparative transcriptome analysis of Trichoderma atroviride IMI206040   during mycoparasitic interaction with the plant pathogenic fungus   Rhizoctonia solani. In this study, transcript fragments of 7,797   Trichoderma genes were sequenced, 175 of which were host responsive.   According to the functional annotation of these genes by KOG, the most   abundant group during direct contact was ''Metabolism''. Quantitative   RT-PCR confirmed the differential transcription of 13 genes (including   swo1, axe1 and homologs to papA, pra1) in the presence of R. solani.   Additional relative gene expression analysis of these genes, conducted   on different stages of mycoparasitism against Botrytis cinerea and   Phytophthora capsici, revealed synergistic transcription of various   genes involved in cell wall degradation. The similarities in expression   patterns and the occurrence of regulatory binding sites in the   corresponding promoter regions suggest a possible analog regulation of   these genes during mycoparasitism of T. atroviride. Furthermore, a   chitin- and distance-dependent induction of pra1 was demonstrated.  Barbara Reithner 1,3*, Enrique Ibarra-Laclette 2, Robert L. Mach 1, and Alfredo   Herrera-Estrella 3    1 Research Area Gene Technology, Institute of Chemical Engineering, Vienna Unive  rsity of Technology, Gumpendorferstraße 1a/166, A-1060 Vienna, Austria  2 Core facility, Laboratorio Nacional de Genómica para la Biodiversidad, Cinvest  av Campus Guanajuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, , Irapua  to 36821, Guanajuato, México  3 Research Area Fungal Development and Gene Expression, Laboratorio Nacional de   Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km 9.6 Libramiento   Norte Carretera Irapuato-León, Irapuato 36821, Guanajuato, México  barbara.reithner@tuwien.ac.at