GSE211530
Chronic exposure to butyl-paraben causes photosensitivity disruption and memory impairment in adult zebrafish
Limited studies on neurotoxicity following chronic exposure to butyl-paraben (BuP) have been conducted. In this study, neurobehavior in zebrafish adults was assessed using a novel tank test, photomotor response tests, and T-maze tests after exposure to BuP for 28 days at the concentrations of 0, 0.01, 0.1, and 1.0 mg/L. To comprehensively understand the underlying molecular perturbations in the brain, alterations in transcripts, neurotransmitters, and neurosteroids were measured. We found that BuP penetrated the blood-brain barrier and impaired neurobehavior in photosensitivity at 1.0 mg/L and in memory at 0.1 and 1.0 mg/L. RNA-seq analysis showed that phototransduction, tight junctions, and neuroactive ligand receptor activity were significantly affected, which explains the observed abnormal neurobehaviors. Neurosteroid analysis revealed that BuP increased cortisol levels in a concentration-dependent manner and specifically reduced allopregnanolone levels at all tested concentrations, suggesting that cortisol and allopregnanolone are significant neurosteroid markers associated with photosensitivity and memory deficits. Collectively, we demonstrated that BuP can cross the blood-brain and modulates the levels of transcripts, associated with phototransduction and circadian rhythm, and neurosteroidal cortisol and allopregnanolone, resulting in abnormal neurobehavioral responses to light stimulation and learning and memory. Overall design: Comparative gene expression profiling analysis of RNA-seq data for control and butylparaben treated groups.