ena-STUDY-AG Botanik & Arboretum-02-06-2017-12:59:57:337-315
Identification and characterisation of viral and bacterial pathogens affecting ulmus laevis
Scientific  findings  indicate thatviruses are  widespread  in  trees  of  forests  and  urban  green, and may cause losses of economic importance. Over many years a monitoring was carried out with over 100 years old European white elms(Ulmus laevis PALL.) in an urban park in eastern Germany. During the last  years, they showed leaf symptoms such as chlorotic ringspots, necroses and dieback, suggesting a virus as causal agent. Fungal  and  bacterial pathogens as  causal  agent  of  these symptoms could  be  excluded by microbiological assays. Also  an  infection by  well  studied viruses such as Cherry leaf roll virus (CLRV), Elm mottle virus(EMV), Arabis mosaic virus(ArMV) and Tobacco ringspot virus(TRSV) could not be confirmed. However, some facts point to a member of the genus Carlavirus. The aim of the present study is the isolation and characterization of a putative viral pathogen which  is  so  far  unknown. Based upon  the  assumption  that  the  causal  agent  belongs  to  the genus Carlavirus, we apply different specific molecular biological and biochemical methods to identify the putative virus.
Scientific  findings  indicate thatviruses are  widespread  in  trees  of  forests  and  urban  green, and may cause losses of economic importance. Over many years a monitoring was carried out with over 100 years old European white elms(Ulmus laevis PALL.) in an urban park in eastern Germany. During the last  years, they showed leaf symptoms such as chlorotic ringspots, necroses and dieback, suggesting a virus as causal agent. Fungal  and  bacterial pathogens as  causal  agent  of  these symptoms could  be  excluded by microbiological assays. Also  an  infection by  well  studied viruses such as Cherry leaf roll virus (CLRV), Elm mottle virus(EMV), Arabis mosaic virus(ArMV) and Tobacco ringspot virus(TRSV) could not be confirmed. However, some facts point to a member of the genus Carlavirus. The aim of the present study is the isolation and characterization of a putative viral pathogen which  is  so  far  unknown. Based upon  the  assumption  that  the  causal  agent  belongs  to  the genus Carlavirus, we apply different specific molecular biological and biochemical methods to identify the putative virus.