GSE158644
RNA sequencing results using Solanum tuberosum cv. Desiree at NT and HT at 3 and 8 weeks
Leaves of the potatoes grown for 3 and 8 weeks at NT and HT were harvested for total RNA extraction. Total RNA was extracted from 100 mg of ground leaves using the Trizol reagent (MRC) following the manufacturer's instructions. Five micro gram of each RNA sample were generated for a strand-specific library as previously described. The library was containing inserts of 150-200 bp. For RNA sequencing, paired-end 150 nt sequencing was performed using a HiSeq 4000 platform (Illumina, USA) at Microgen (Korea). Overall design: Examination of differential expression of genes at NT and HT at 3 and 8 weeks