DRP000188
Comprehensive identification and characterization of the binding sites of HIF-1alpha
Although recent studies have revealed that the majority of human genes are subjected to regulation of alternative promoters (APs), the biological relevance of this phenomenon remains unclear. To enable more comprehensive TSS analysis in the respective cell types, we recently developed a method, combining oligo-capping with the massively paralleled sequencing technology, Illumina GA. In this method, which we named TSS Seq, sequence adaptor which is necessary for Illumina GA sequencing is directly introduced to the cap site of the mRNA. By sequencing 36-48 sequence immediately downstream of the TSSs (TSS tags), it is possible to obtain precise positional information of transcriptional start sites (TSSs). In this paper, we used the TSS tag data accumulated from twelve different cell types and normal tissues in humans for the identification and characterization of the APs in human genes.
Comprehensive identification and characterization of the binding sites of HIF-1alpha in mammalian genes were attempted. We used ChIP-Seq method, in which next gene sequencing technology and chromatin-immunoprecipitation assay were combined.