ena-STUDY-INRA (Nouvelle-Aquitaine-Bordeaux)-28-03-2019-13:35:18:340-137
Sequence based microsatellite genotyping development for Alosa alosa and Alosa fallax.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach. A total of 28 loci were amplified in a multiplexed PCR and sequenced for 156 individuals (from A. alosa, A. fallax and their hybrids) that were repeated to estimate genotyping error. Data were used as a proof of concept of the method and 24 high quality loci were validated. This method has then been applied to genotype additional individuals.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach. A total of 28 loci were amplified in a multiplexed PCR and sequenced for 156 individuals (from A. alosa, A. fallax and their hybrids) that were repeated to estimate genotyping error. Data were used as a proof of concept of the method and 24 high quality loci were validated. This method has then been applied to genotype additional individuals.