GSE129575
Defining genetic variation in widely used congenic and backcrossed mouse models reveals varied regulation of genes important for immune responses [RNA-seq]
In our study, we developed an alternative next-generation sequencing dataset processing strategy to identify genetic variation in backcross mouse models.  We used this approach to define SJL genetic content in B6.SJL-CD45.1 mice from different vendor sources. Overall design: We performed RNA-seq experiments on CD4+ Th1 cells isolated from C57BL/6 or B6.SJL-Ptprca Pepcb/Boy mice from three different vendor sources.  We also performed RNA-seq experiments on CD4+ Th1 cells isolated from Tbx21-/- mice.