HMP_16S_Development_Mock_Community
Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: 454 Impact of PCR on Chimera Formation - Mock
The impact of PCR conditions on chimera content was evaluated by pyrosequencing the HMP even and staggered Mock community. The following PCR parameters were varied: cycle number (20x, 30x, or 40x), template genomic DNA concentration (0.1 ng, 1 ng, or 10 ng), and extension time (1 min. or 5 min.)