GSE201081
The Transcript Integrity Index (TII) provides a standard measure of sperm RNA
Human sperm RNA-seq was performed and sequences used for the generation of a RNA integrity index capable of assessing samples with high RNA fragmentation.  A two-prong approach was used including both manual and computational analysis.  This identified a series of sperm RNAs that are consistently intact, and acceptable for use in determining sample RNA quality.  These RNAs function in roles related to spermatogenesis and/or fertilization. Overall design: Samples were human sperm from the male partner of couples seeking fertility treatment.