GSE132051
Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates
We engineered m6A methyltransferase or demethylases with CRISPR-Cas9 to achieve site-specific editing of m6A. The resultant m6A editors can be programmed with a guide RNA, allowing functional comparison of single site methylation in different mRNA regions. Overall design: Examination of m6A methylation patterns after targeted methylation of Actin and targeted demethylation of Malat1.