GSE134274
Transcriptomics of AML12 transfected with PAV-TBG-P2A-GFP-Chrna4 over-expression plasmid
We reported the transcriptomics responses from RNA-seq of AML12 transfected with Chrna4 over-expression plasmid. We used cultured AML12  from 3 to 5 generations and transfected it with pCMV6-AC-GFP or PAV-TBG-P2A-GFP-Chrna4 plasmid. After 24 h, total RNA of cultured AML12 was extracted with RNAiso Plus (TaKaRa). Then we delegated it to Novogene company for RNAseq aiming to identify key factors promoting this transition. Overall design: Examination of AML12 transcriptomics after being transfected with pCMV6-AC-GFP or PAV-TBG-P2A-GFP-Chrna4 plasmid