GSE171881
RNA-Seq analysis of human M238P melanoma cells transduced with a lentivirus expressing the miR-143/145 cluster or a control lentivirus
To assess the transcriptomic response associated with upregulation of the miR-143/-145 cluster, BRAFV600E mutant human melanoma cells M238P cells were transduced for stable expression of miR-143/145 cluster or with a control vector. Forty-eight hours after transduction, cells were treated with 1 µg/mL of puromycin for one week. RNA samples were then harvested. Two independent experiments were carried out. Overall design: 2 samples in duplicates: control versus miR-143/-145