PRJNA731329
Chromosome-scale genomes of wild mulberry highlight the evolution of karyotype, sex determination, adaptation and domestication of mulberry
We collected 15 representative wild accessions from Spain, Sri Lanka, China and other countries. Together with the publicly available genomic sequences for landraces, genetic divergences among multiple wild and cultivated species were studies. In these samples, 6 wild mulberry trees are mainly from the Southwest of China. 7 semi-wild mulberries from other regions. The leaf was sampled from wild mulberries, and 10 micrograms of genomic DNA prepared by standard CTAB extraction protocol was used to construct the library of 150 bp and 100 bp in length, respectively. Sequence libraries were constructed according to the Illumina library preparation protocol and sequenced on the Illumina Hiseq platform and BGI platform to generate 150 bp and 100 bp paired-end reads, respectively.