PRJNA729878
Short Plus Long-Amplicon Based Sequencing Approach Improves Genomic Coverage And Variant Detection In SARS-CoV-2 Genome
We designed long amplicon primers and evaluated the performance of short (ARTIC) plus long amplicon (MRL) based sequencing approach to detect the mutations. We optimized the sequencing assay on VR-1986D-ATCC RNA and then analyzed nasopharyngeal swab specimens from five COVID-19 positive (RT-PCR ct values <30) patients.