GSE169111
Genome-scale CRISPR screen identifies TMEM41B as a critical host factor required for coronavirus replication [PigGeCKO]
We use the a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO)  library (Zhao et al., 2020)  to identify key host factors facilitating TGEV infection in porcine cells. Overall design: We generated a PigGeCKO cell collection (in the PK-15 background) as previously described  and performed a genome-wide loss-of-function genetic screen.Briefly, we conducted three rounds of TGEV challenge, employing untreated PK-15-Cas9 cells as a negative control.