GSE174766
Genome wide CRISPR screen for CG-806 resistance in OCI-LY3 cells using Y. Kosuke library
OCI-LY3 cells were infected with CRISPR/Cas9 library, selected for puromycin resistance for integration events and exposed to CG-806  or vehicle, DMSO, at 1 microMolar concentrations.  Cells were collected at time 0 (post puromycin selection) and day 7. DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Novaseq 6000. Overall design: Performed comparisons of sgRNA read counts between DMSO and CG-806  treated cells at corresponding time points.