GSE202783
Labelling transcriptional response to dissociation in mouse hippocampus using scSLAMseq
Transcriptional response to dissociation is known to cause artefacts in single cell data sets. We used 4sU to label newly made transcripts during dissociation in order to to later remove them from the data set Overall design: 4sU was added to the dissociation buffer while the hippocampi were enzymatically dissociated for 30 min.