Carrot EST
Daucus carota subsp. sativus Transcriptome or Gene expression
We combine Sanger sequencing and ultra-high throughput sequencing to develop an annotated EST/gene sequence database for carrot. This sequence serves as a reference to identify Single Nucleotide Polymorphism (SNP) or insertion/deletion (Indel) variation in breeding germplasm of carrot using Illumina technology. The EST database serves as a reference for other Apiaceae species. The sequences and markers developed will form the basis for the first extensive gene-based genetic maps in this family.