ena-STUDY-UNIVERSITY OF OSLO-23-02-2019-11:14:36:701-7
RNAseq of 48 Atlantic cod juveniles. Time-series of a Francisella noatunensis infection with parallell controls 6hrs, 2, 4 and 7 days post intraperitonal injection
The Francisella noatunensis subsp. noatunensis NCIMB 14265 isolate used for challenge was originally isolated from diseased Atlantic cod (Gadus morhua) in Norway, and was provided by Dr. Duncan Colquhoun at the National Veterinary Institute Oslo, Norway. The fish were acclimated to 15 ºC and starved 24 h before injection. Prior to intra-peritoneal (ip) injection, the fish (approx. 25 g) were anaesthetised with Metacainum (50 mg/l, Norsk Medisinaldepot), and injected with 100 µl of either F. noatunensis (5 x 107 cfu per fish) or 0.9 % NaCl (control). Head kidney from 6 individuals was sampled at 6 hours, 2, 4 and 7 days post challenge from both the treated and untreated groups. All libraries were prepared using the TruSeq™ RNA low-throughput (LT) protocol (Illumina), non-stranded. All libraries were sequenced 100bp paired-end (PE) at the Norwegian Sequencing Centre on the Illumina HiSeq 2000
The Francisella noatunensis subsp. noatunensis NCIMB 14265 isolate used for challenge was originally isolated from diseased Atlantic cod (Gadus morhua) in Norway, and was provided by Dr. Duncan Colquhoun at the National Veterinary Institute Oslo, Norway. The fish were acclimated to 15 ºC and starved 24 h before injection. Prior to intra-peritoneal (ip) injection, the fish (approx. 25 g) were anaesthetised with Metacainum (50 mg/l, Norsk Medisinaldepot), and injected with 100 µl of either F. noatunensis (5 x 107 cfu per fish) or 0.9 % NaCl (control). Head kidney from 6 individuals was sampled at 6 hours, 2, 4 and 7 days post challenge from both the treated and untreated groups. All libraries were prepared using the TruSeq™ RNA low-throughput (LT) protocol (Illumina), non-stranded. All libraries were sequenced 100bp paired-end (PE) at the Norwegian Sequencing Centre on the Illumina HiSeq 2000