GSE134799
ChIP assay in adult ventricular cardiomyocytes
ChIP assay was performed to study the genomic location of SRF myocytes cistrome in adult ventricular cardiomyocytes. The myocytes were stimulated by phenylephrine (PE) combined with adenoviral overexpression of SRF serine-103 mutants. Overall design: Adult ventricular cardiomyocytes were stimulated with PE 24 hours after adenoviral infection with SRF WT, S103A and S103D mutants. Cells were fixed 1 hour after PE treatment, then subjected to Chromatin Immunoprecipitation (ChIP) by PolII, H3K27ac, Flag, SRF and p-SRF(s103) antibodies.