Whole-genome sequencing of a laboratory-evolved yeast strain CA0001
Whole-genome sequencing of a laboratory-evolved yeast strain CA0001
The goal of this study was to demonstrate the power of high-throughput sequencing technologies in uncovering, at the single-base and mega-base scale, genetic adaptation in experimental evolution in Saccharomyces cerevisiae.
We sequenced >93.5% of the genome of a laboratory-evolved strain of the yeast Saccharomyces cerevisiae and its ancestor at >28x depth. Both single nucleotide polymorphisms and copy number amplifications were found, with specific gains over array-based methodologies previously used to analyze these genomes. Applying a segmentation algorithm to quantify structural changes, we determined the approximate genomic boundaries of a 5x gene amplification. These boundaries guided the recovery of breakpoint sequences, which provide insights into the nature of a complex genomic rearrangement.