ena-STUDY-INRA (Nouvelle-Aquitaine-Bordeaux)-28-03-2019-13:56:29:851-141
Sequence based microsatellite genotyping development for Armillaria species.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach. A total of 51 loci were amplified in a multiplexed PCR and sequenced for 288 individuals and 96 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 38 high quality loci were validated.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach. A total of 51 loci were amplified in a multiplexed PCR and sequenced for 288 individuals and 96 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 38 high quality loci were validated.