ena-STUDY-LEIBNIZ INSTITUTE FOR AGE RESEARCH - FRITZ LIPMANN-16-06-2016-12:36:48:388-47
Isoform sequencing of barley (Hordeum vulgare), using the reference cultivar 'Morex'.
Transcriptome sequencing with short read technology usually involves either de novo assembly or mapping of the reads to a reference sequence. Both of these approaches can result in bioinformatics artefacts that lead to the output of fragmented transcripts.  To overcome this problem we chose a Pacific BioSciences IsoSeq approach which can theoretically yield full-length transcripts, without the need for assembly or read mapping. We applied this approach in barley (Hordeum vulgare), using the reference cultivar 'Morex'.
Transcriptome sequencing with short read technology usually involves either de novo assembly or mapping of the reads to a reference sequence. Both of these approaches can result in bioinformatics artefacts that lead to the output of fragmented transcripts.  To overcome this problem we chose a Pacific BioSciences IsoSeq approach which can theoretically yield full-length transcripts, without the need for assembly or read mapping. We applied this approach in barley (Hordeum vulgare), using the reference cultivar 'Morex'.