PRJNA471053
Transcriptome of human primary CD4+ T cells before and after HIV infection, quiescence and reactivation
To define the pattern of gene expression changes in human primary CD4+ T cells during the different stages of HIV life cycle, they were transfected with an HIV construct containing a CD8a-GFP reporter gene which allowed purification of HIV-infected cells. The purified HIV+ population was cultured in step-down media resulting in induction of quiescence. Fully quiescent cells were reactivated through TCR stimulation. Samples were taken for RNA-seq from mock-infected cells and purified HIV+ cells 72 hours after infection, and from fully quiescent cells and 24 hours after TCR stimulation.