GSE160762
Next Generation Sequencing of Cells Derived from Directed Differentiation of Wild Type and E2A Knockout Human ESCs into Cardiomyocytes
This study aimed  to compare mRNA profiles of cells derived from directed differentiation of wild type and E2A knockout human ESCs  into cardiomyocytes. Using an optimized data analysis workflow, we  identified 34,800 transcripts in  WT and E2A -/-  cardiac mesoderm cells (day3), early cardiomyocytes (day7) and later cardiomyocytes (day15). The transcripts showed 1889 differential expression genes (1099 upregulated and 790 downregulated) between the WT and E2A -/- cells, with a fold change =2.0 and p value <0.05.  Hierarchical clustering of differentially expressed genes  discovered that these genes may contribute to related phenotypes of E2A knockout cardiomyocytes. Overall design: mRNA profiles of wild type and E2A knockout cells from day3, day7 and day15 during human ESCs (H7)-cardiomyocytes directed differentiation were generated by deep sequencing, using Illumina HiSeqX10.