PRJNA526543
Trypanosoma brucei strain:Lister 427 Genome sequencing
To provide evidence that the Trypanosoma brucei vault RNA is transcribed by RNA polymerase III (Pol III), we performed ChIP-seq. One of the alleles for the largest subunit of Pol III (Tb927.10.2780) was tagged with a Ty1 epitope (EVHTNQDPLD)-mCherry fusion and the second allele was deleted. After formaldehyde cross-linking and immunoprecipitation of chromatin fragments with monoclonal antibody against the Ty1 epitope, libraries for high-throughput sequencing were generated and processed on the Illumina platform.