Comparative_global_transcriptional_profiling_of_blood_stage_Plasmodium_falciparum_3D7_parasites_that_are_enriched_or_depleted_of_reactivity_to_a_human_monoclonal_antibody_that_carries_the_RIFIN_specific_LAIR1_insertion_-sc-4997
Comparative_global_transcriptional_profiling_of_blood_stage_Plasmodium_falciparum_3D7_parasites_that_are_enriched_or_depleted_of_reactivity_to_a_human_monoclonal_antibody_that_carries_the_RIFIN_specific_LAIR1_insertion_
Synchronized, asexual blood-stage Plasmodium falciparum 3D7 parasites that have undergone two and three rounds of positive (3D7-MGD21+2 and 3D7-MGD21+3), and negative (3D7-MGD21-2 and 3D7-MGD21-3) selection on MGD21, a broadly reactive human monoclonal antibody with a LAIR1 insertion that is specific for RIFIN antigens, were used to infect red blood cells. Parasite RNA samples were collected at seven different time points post-infection (post-sorbitol synchronization at early ring stage) (0, 8, 16, 24, 32, 40 and 48 hours), encompassing the entire intraerythrocytic developmental cycle from ring to mature schizonts.  Using RNA sequencing, we aim to determine (a) the full subset of RIFINs that are recognized by the LAIR1-containing monoclonal antibody, and (b) whether there are any significant global transcriptional differences between parasites that have been enriched or depleted of reactivity to the LAIR1-containing antibody.\r\nThis data is part of pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http//:www.sanger.ac.uk/datasharing
Comparative transcriptomics of blood-stage P. falciparum 3D7 parasites that have been enriched or depleted of reactivity to a broadly reactive human monoclonal antibody that carries a human LAIR1 insertion, which binds to RIFIN antigens.