GSE133067
Cryptococcus neoformans reactivation in different media and temperatures
We wanted to find out the transcriptomic response of growth-arrested Cryptococcus yeast cells when initially placed in environments in which they could reactivate or "wake up", focusing on the difference between a rich microbial media (YPD) and a common cell culture media, RPMI + 10% heat-inactivated fetal calf serum. Please contact Edward.Wallace@ed.ac.uk if you use this data and ask for citation information; we are currently (August 2020) working on a publication. Overall design: 36-sample RNA-seq timecourse of reactivation of growth-arrested Cryptococcus neoformans H99 in two media at two temperatures. Two biological samples of cells were pre-grown for 5 days in 10mL YPD. A sample of this was taken as the "0 minute" control timepoint. We then inoculated to OD 0.1 in 4 different cultures of 100mL pre-warmed media: (YPD, RPMI+) x (25C, 37C), and grew for 10, 30, 60, or 120 min. We also obtained a timepoint in more standard growth conditions of YPD, 30C, 180mins.