GSE192934
Differential Expression Analysis of a Histidine phosphotransferase RNAi mutant in Magnaporthe oryzae
Magnaporthe oryzae is notorious for its efficient evolution as a plant pathogen.  Analysis of well organised fungal pathogenic mechanisms would enable the scientific  understanding to fight against pathogens. Histidine phosphotransferase in fungal  systems are good targets for antifungals since they are component of SAPK pathway,  which is essential for survival of the fungus. The histidine Phosphotransferase YPD1  in M. oryzae is known to be light responsive and important for both asexual  reproduction as well as pathogenicity. In this study, the knock-down (KD) mutant of  YPD1 was highly sensitive to cell wall perturbing agents and were highly deficient in  laccase expression and activity. A RNA-seq transcriptome screening of KD mutant  compared to wild type revealed altered expression of about 146 genes important in  biotrophic and necrotrophic phases of the pathogen. About 96 genes were found to be  down regulated, of which several plant cell wall degrading genes vital for pathogenicity  and fungal cell wall maintenance genes important for growth and fungal development  were identified. Most of the genes identified are involved in biotrophic invasion and  metabolism. Our results explain the reason for the deficiency of the KD mutant in  asexual reproduction as well as pathogenicity. Analysis of differentially expressed  genes in the KD YPD1 mutant suggests that YPD1 can be a good target for  development of novel antifungal compounds, as YPD1 expression affects genes  required for pathogenicity. Overall design: Two pooled samples of B157 and RA6 were analysed,  B157 is the wildtype control sample RA6 is the RNAi mutant of MoYPD1 in M.oryzae. There is no biological replicates