GSE126660
Epidermal basal cell-specific in vivo ribosome profiling to describe the translation landscape of HrasG12V and Eif2b5
eIF2B5 is necessary for HrasG12V hyperplastic growth.  Ribosome profiling was used to describe HrasG12V and WT dependence on eIF2B5-mediated translation. Overall design: E9.5 HrasG12V and WT mouse epidermis was transduced by in utero injection with shScrambled control or shEif2b5. E18.5 epidermal basal cells were enriched for ribosome profiling and RNAseq.