GSE184038
Distinct Patterns of Insulin response, sexual development, organ size regulation in postnatal developing Subcutaneous and Visceral fat of Bama Pig
In this study, the SAF and VAF development was characterized and compared in SAF and VAF of Bama pig during postnatal development (infancy, puberty and adulthood) using RNA sequencing technologies (RNA-Seq). We profiled the transcriptome of SAF and VAF isolated from 1-, 3- and 6-month-old pigs and identified 23636 expressed transcription units. Of the them, 1165 genes were differentially expressed between the despots and /or developmental stages. Upregulated gene in SAF of Bama pigs showed significant function and pathway enrichment in central nervous system development, lipid metabolism, oxidation-reduction process, cell adhesion while genes involved in immune system process, actin cytoskeleton organization, male gonad development, hippo signaling pathway preferentially expressed in VAF. Short time-series expression miner analysis demonstrated the differentiation in gene expression patterns between the two deposit was corresponding to their distinct responses in sextual development, hormone signaling pathway, lipid metabolism as well as hippo signaling pathway. Transcriptome analysis of SAF and VAF in Bama pigs suggested that the depot differences of the adipose tissue are not only related to lipid metabolism and endocrine function, but also closely associated with sextual development and organ size regulation. Overall design: Subcutaneous fat (SAF) fand Visceral fat (VAF) mRNA profiles of Bama pig during postnatal development (infancy, puberty and adulthood; 1months, 3months and 6months) were generated RNA sequencing technologies (RNA-Seq). Nine cDNA libraries representing SAF-1, SAF-3, SAF6, VAF1, VAF3, VAF6 groups were constructed for mRNA transcriptome analysis.