GSE184778
Identification of the effect of AhR antagonists on the physiology of human HSPC.
We demonstrated to examine the effect of AhR antagonists on the specific lineage-biased differentiation of HSPC. To confirm this, CD34+ cells isolated from cord blood were cultured with two antagonists (CH223191 and StemRegenin 1) for 14 days to monitor their phenotype, and chromatin immunoprecipitation DNA was extracted from cultured cells on the 14th day of culture. We sought to explain the relationship between the action of AhR-antagonist complex and the megakaryocyte lineage-biased differentiation of HSPC with these ChIP-seq results. Overall design: Examination of 2 different Ary hydrocarbon receptor antagonists in cord blood (CB)-HSPCs (DMSO control vs. CH223191-treated vs. StemRegenin1-treated).