GSE131306
The effects of chemokines CXCL1/2/8 on mouse lung fibroblasts
Our preliminary data show that the primary triple-negative breast tumor-derived chemokines CXCL1/2/8 promote migration and tube formation of mouse lung endothelial cells, which is mediated by lung fibroblasts. To explore the molecular events underlying this mediator function of lung fibroblasts, we treated freshly-isolated lung fibroblasts with chemokines CXCL1/2/8 and performed RNA-Seq assay. Overall design: Freshly-isolated mouse lung fibroblasts were treated with recombinant human CXCL1 (8 nM)/2 (2 nM)/8 (20 nM) proteins for 3 hours.