E-MTAB-10347
RNA-seq of human embryonic stem cells (hESC) line Amicqui-1 derived and maintained on human amniotic epithelial cells
Conventional conditions to maintain human embryonic stem cells (hESC) imply the use of inactive mouse embryonic fibroblast (iMEF) as a feeder layer. However, it has suggested that the culture of hESC on iMEF could be an artifact that does not correspond to the in vitro counterpart of the human epiblast. We previously derived and maintained human embryonic stem cells (Amicqui-1 hESC line) on a feeder layer of human amniotic epithelial cells (hAEC). However, the mechanisms involved in the interaction between both cell types to promote the pluripotency still remain unknown. To elucidate if the transcription profile of hESC on hAEC differs from conventional conditions on iMEF, we carried out RNA-seq of Amicqui-1 hESC on both feeder layer conditions (AMIQ hESC-iMEF and AMIQ hESC-hAEC) and on their respective conditioned media (AMIQ hESC-hAEC-CM and AMIQ hESC-iMEF-CM). In this experiment we included H1-hESC-iMEF and hAEC alone.
Conventional conditions to maintain human embryonic stem cells (hESC) imply the use of inactive mouse embryonic fibroblast (iMEF) as a feeder layer. However, it has suggested that the culture of hESC on iMEF could be an artifact that does not correspond to the in vitro counterpart of the human epiblast. We previously derived and maintained human embryonic stem cells (Amicqui-1 hESC line) on a feeder layer of human amniotic epithelial cells (hAEC). However, the mechanisms involved in the interaction between both cell types to promote the pluripotency still remain unknown. To elucidate if the transcription profile of hESC on hAEC differs from conventional conditions on iMEF, we carried out RNA-seq of Amicqui-1 hESC on both feeder layer conditions (AMIQ hESC-iMEF and AMIQ hESC-hAEC) and on their respective conditioned media (AMIQ hESC-hAEC-CM and AMIQ hESC-iMEF-CM). In this experiment we included H1-hESC-iMEF and hAEC alone.