PRJNA799907
Test the editing efficiency of AsCas12a and SpCas9 in different settings
We compared AsCas12a-mediated LAHR with traditional SpCas9-mediated HDR in two endogenous genes: ALK and CACNA1D. Besides, we also explored if the location of the nucleotide substitution could influence the LAHR efficiency in HAP1-deGFP reporter cell line.