GSE161150
Next generation sequencing of transcripts associated to five selected RNA-binding proteins in Leishmania infantum
We report the sequencing of mRNAs associated with three Leishmania infantum Poly (A) binding proteins (PABP1, PABP2 and PABP3) and two of their partner RNA binding proteins (RBP23 and DRBD2). PABP1 and RBP23 preferentially associate with mRNAs that encode ribosomal proteins, absent from DRBD2-associated transcripts, and differently from PABP2 and PABP3 that bind to an overlapping set of mRNAs. Overall design: mRNAs co-precipitated with HA-tagged RNA-binding proteins from cytoplasmic extracts of transfected Leishmania infantum cells