Melon solid
SOLiD Sequencing for SNP discovery across Cucumis melo
The deep transcriptome re-sequencing of all genotypes grouped in 8 pools (wild African agrestis, Asian agrestis and acidulus, exotic Far Eastern conomon, Indian momordica and Asian dudaim and flexuosus, commercial cantalupensis, subsp. melo Asian and European landraces, Spanish inodorus landraces, and Piel de sapo breeding lines) yielded about 300 M reads. Short reads were mapped to the recently generated draft genome assembly of the DHL line Piel de sapo (inodorus) x Songwhan Charmi (conomon).
Melon (Cucumis melo) is a highly diverse species cultivated worldwide. Recent advances in massively parallel sequencing are allowing the study of nucleotide diversity in this species. As a first step to extensively document levels and patterns of nucleotide variability across the species, we used the high-throughput SOLiD™ system to re-sequence the transcriptomes of a set of 67 genotypes previously selected from a core collection representing the whole species extant variation.