GSE195704
The influence of gingipains on latent capacity of B cells
Porphyromonas gingivalis (P. gingivalis) is a key pathogen in periodontitis. Our previous study indicated that periodontitis induced by P. gingivalis increases the percentage of CD19+ B cells, Th17, Treg, gMDSCs and mMDSCs but decreases the ratio of B10 cells in CIA mice. It's unclear which virulence factors of P. gingivalis are involved in these processes. Here, our study first indicated that such effects are mainly resulted from the undenatured proteins other than the DNA, RNA or LPS of P. gingivalis. As gingipains are enzymes and virulence factors which play vital role in the progression in periodontitis through affecting innate and adaptive immune system, we then compared the influence of the wild-type strain of P. gingivalis (ATCC33277) with its isogenic gingipain-null mutant (?K?RAB) on B cells. The results showed that B cells infected with ?K?RAB exhibited increased levels of IL-6 and others cytokines (IL-1ß,IL-23) involve in Th17 differentiation compared to wild-type strain, also the frequency of IL-10 producing regulatory B cells (B10). Furthermore, the peritonitis induced by ?K?RAB enhanced the proportion of B10 and Th17 compared with treated with ATCC33277. Cumulatively, this study preliminarily revealed deletion of gingipains affected the antigen presentation of B cell that promoted B cells express the cytokines which promoted T cells toward Th17 and the secretion immunosuppressive cytokines of B cells that enhanced the frequency of B10 which implicated gingipains are vital in altering B cell function to participated immune response. Overall design: mRNA profiles of B cells treated with Porphyromonas gingivalis and its gingipain-null-mutant