Parallel, tag-directed assembly of locally derived short sequence reads
Parallel, tag-directed assembly of locally derived short sequence reads
Proof-of-concept for subassembly methodology on P. aeruginosa PAO1 and Lake Washington methylamine-enriched metagenomic community.
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.