GSE131178
Molecular fingerprint of bovine embryos produced by superovulation with high competence to establish pregnancy
The objective of the current experiment was to identify the transcriptome profile of in vivo-derived embryos with high competence to establish and maintain gestation. Using P < 0.01 and a fold change > 2-fold or < 0.5 fold as cut-off, there were 837 DEG (789 upregulated and 48 downregulated) between embryos that survived to day 60 versus those that did not. Using a more stringent criteria, a FDR < 0.05 and a fold-change of > 2-fold or < 0.5, there were 558 DEG (505 upregulated and 33 downregulated) between embryos that survived to day 60 versus those that did not. Overall design: Blastocysts produced from superovulated Holstein heifers were flushed from the uterus 8 days after anticipated ovulation and bisected. One demi-embryo from a single blastocyst was transferred into a synchronized recipient and the other half was used for RNA-seq analysis by Illumina NextSeq. Recipients were diagnosed for pregnancy at day 30 and 60 of gestation. All cows diagnosed as pregnant at day 30 maintained pregnancy until day 60.