GSE67132
Genome-wide mapping of H3K27Me3 in mouse rib chondrocytes
We performed ChiP sequencing analysis for H3K27Me3 in DNA from mouse rib chondrocytes. Overall design: Chromatin immunoprecipitation for H3K27Me3 in mouse rib chondrocytes after overnight culture.Chromatin Immunoprecipitation was performed using Cell Signaling Technology SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnentic Beads) #9005 Sequencing using Next Generation Sequencing (Otogenetics), mapping of reads using DNA Nexus mapper and region calling using Quest software (DNA Nexus)