GSE41232
GSE41232: Adaptive evolution of Corynebacterium glutamicum resistant to oxidative stress and its global gene expression profiling
Summary:   Corynebacterium glutamicum was adapted in a chemostat for 1,900 h with gradually increasing H2O2-stress to understand the oxidative stress response of an industrial host. After 411 generations of adaptation, C. glutamicum developed the ability to grow under stress of 10 mM H2O2, whereas the wild-type did not. The adapted strain also showed increased stress resistance to diamide and menadione, sodium dodecyl sulfate, Tween 20, hydrochloric acid, sodium hydroxide, and ampicillin. A total of 1,180 genes in the RNA-seq transcriptome analysis of the adapted strain were upregulated twice or higher (corresponding to 38.6% of the genome), and 126 genes were downregulated half or less (4.1% of genome) under 10 mM H2O2-stress conditions compared with those of the wild-type under a no stress condition. Aromatic compound-degrading gene clusters (vanRABK, pcaJIRFLOI, and benABCDRKE) were more than three-fold upregulated, and intracellular accumulation of protocatecheuic acid, an intermediate in the aromatic compound degradation pathway, was found. Plausible reasons for the H2O2-stress tolerance of the adapted strain are discussed. Overall Design: Comparison of global gene expression profiling using RNA-seq data of wild-type strain under normal condition (WT_MCGC) vs. 10 mM H2O2-adapted strain under oxidative stress conditionGlobal gene expression profiling_WT vs. H2O2 adapted C. glutamicum strains