PRJNA472087
High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues
Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing high mortality in farmed Atlantic salmon (Salmo salar). The collective data from the Atlantic salmoneISAV interactions, performed “in vitro” using various salmon cell lines and “in vivo” fish infected with different ISAV isolates, have shown a strong regulation of immune related transcripts during the infection. Despite this strong defence response, the majority of fish succumb to infections with ISAV. The deficient protection of the host against ISAV is in part due to virulence factors of the virus, which allow evade the host-defence machinery. As such, the viral replication is uninhibited and viral loads quickly spread to several tis- sues causing massive cellular damage before the host can develop an effective cell-mediated and hu- moral outcome. To interrogate the correlation of the viral replication with the host defence response, we used fish that have been infected by cohabitation with ISAV-injected salmons. Whole gene expression patterns were measured with RNA-seq using RNA extracted from Head-kidney, Liver and Gills. The re- sults show divergent mRNA abundance of functional modules related to interferon pathway, adaptive/ innate immune response and cellular proliferation/differentiation. Furthermore, gene regulation in distinct tissues during the infection processwas independently controlled within the each tissue and the observed mRNA expression suggests high modulation of the ISAV-segment transcription. Importantly this is the first time that strong correlations between functional modules containing significant immune process with proteineprotein affinities and viral-segment transcription have been made between different tissues of ISAV-infected fish.©