PRJNA863432
Clinical observation of metronidazole combined with amoxicillin in the treatment of periodontitis and changes of oral microbial diversity during treatment
Periodontitis is an inflammatory disease around soft tissues of teeth, and the use of antibiotics can effectively solve this problem. In this study, Basic treatment with metronidazole (MTZ) and amoxicillin (AMX) combination drugs may reduce inflammation to maintain oral colony balance. Clinical trial was conducted in 8 patients, and all of the patients were treated with scaling and root planning (SRP) and used of AMX /MTZ (500/400 mg3* per day for 7 days). Each patient received at least 4 sites, the clinical parameters of bleeding on probing (BOP), probing depth (PD) with more than 5mm and clinical attachment level (CAL) and tooth loss degree (LD) were examined until 6 weeks. The high throughput sequencing method was adopted to evaluate the oral microbial diversity. The results showed that there was a significant improvement in CAL and PD parameters with the MTZ /AMX drug treatment compared to the post-treatment group. There was a greater reduction of BOP and statistical significance was less than 0.05 (P<0.05). High throughput sequencing results indicated that the proportion of Gram-negative bacteroidetes Clostridium and porphyromonas decreased over time, and all the periodontitis recovered their oral bacterial flora after 6 weeks treatment. So the use of systemic antibiotics treated chronic periodontitis was very effective, no surgical periodontal treatment, which may decrease the patient pain and lower the costs of repeated treatment. This way may cause less damage to dental tissues and decreased the recurrence of periodontitis in a short time.