GSE112166
STAT5 activation enhances Paneth cell function by regulated ileal Wnt3a-Tcf4-Sox9 or Gfi1 to sustain niche differentiation.
To determine the potential molecular mechanisms by which STAT5 signaling control ileal Paneth cell homeostasis, we isolated total RNA from ileal intact crypts of STAT5+/+, STAT5DIEC-/- and STAT5DIEC+++ mice and performed RNA sequencing (RNA-seq). With an average of 22.3 million reads per sample, we observed 27540 transcripts when reads were aligned to the mm10 genome with annotations provided by Ensembl.  Transcripts were filtered, requiring at least 3 reads in 50% of samples within at least one condition, leaving 10197 transcripts for analysis.  To identify differentially-regulated transcripts, we performed ANOVA (FDR-corrected p<0.05) and required the fold change to exceed 1.5. Overall design: Sincle-end RNA sequencing of total RNA extracted from ileal crypts obtained from Stat5 knockout, wildtype, and overexpressing mice.