PRJNA417171
Expression profiling by high throughput sequencing among wild type and mt2 null before and after infection
To investigate the role of Dnmt2 in conferring immunity against bacterial infection in Drosophila melanogaster, we perform Escherichia coli infection in 1-3 day old males and observed variation in survival. Using genome-wide sequencing analysis, we sequenced wild type and mt2 null flies pre and post 6 hours of infection. Transcriptional profiling showed that there are inherent differences between wild and null flies even before infection. After infection both these flies showed induction of the immune response genes but with a higher extent in null flies, suggesting inflammatory responses in these flies after infection at a young age. This study suggests that absence of dnmt2 causes changes in the expression profile of an organism and it also provides novel insights into the determinants underlying the innate immune response in flies. It also reveals how loss of Dnmt2 can cause transcriptional variation in turn mediating overt physiological differences an thus determining infection susceptibility of an organism.