GSE168054
Transcriptome analysis of nascent hematopoietic cells II
During ontogeny, HSCs or progenitors are generated from endothelial cells through the process known as endothelial-to-hematopoietic transition (EHT). After EHT, hematopoietic cells form cell aggregates, called hematopoietic clusters. To obtain mechanistic insight into HSC specification, we compared the gene expression profiles of hematopoietic clusters between caudal half region and yolk sac. Overall design: Hlf+ nascent hematopoietic cells (c-Kit+CD45-) were isolated from embryonic day (E)10.5 caudal half and yolk sac, and were subjected to RNA-seq analysis.