GSE193650
Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum
We identified a GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the spider Parasteatoda tepidariorum. fuchi transcript is initially expressed in cells at both polar regions of the embryo and later in endodermal and extraembryonic cells. To genome-widely identify genes whose expressions are regulated by fuchi, we conducted RNA-sequencing of whole embryos at three different early stages (stages 2 and 3 and early stage 5) that were untreated (control) or treated for fuchi parental RNA interference (pRNAi). Overall design: We extracted poly(A) mRNA from whole embryos at stage 2 (st2, 11 h after egg laying [AEL]), stage 3 (st3, 20 h AEL), and early stage 5 (st5e, 31 h AEL) that were prepared from the same mated females before and after treatment for fuchi pRNAi. The mRNAs were sequenced using the Illumina Miseq platform to identify differentiallly epxressed genes (DEGs) between the untreated and treated embryos for each stage.  Two biological replicates were obtained for each sample type. We confirmed that the fuchi gene (aug3.g26874) itself was expectedly included in the detected DEGs for all the stages.