Flying_Insect_Long_Read-sc-6380
Flying_Insect_Long_Read
This study aims to determine the optimal lysis buffer, and storage conditions for achieving the best yield and quality DNA for both long read sequencing for marmalade hoverfly population genomics and for short read DNA-barcoding of thousands of malaise trap caught flying insects. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Malaise traps are a cost and time effective way of sampling thousands of flying insects. This project will utilise malaise traps and DNA-barcoding uncover species diversity and interaction. In addition specially selected malaise caught specimens will be submitted for long read sequencing, with a particular interest in UK hoverfly populations which have been declining since the 1980s. The malaise trap project has already provided and will continue to provide a biobank of flying insect material for ongoing research and development works in order to determine optimal buffer/preservative and storage conditions to achieve the best sequencing results.