ena-STUDY-QMUL-17-05-2018-16:31:32:824-190
TO BE DELETED Whole genome shotgun bisulphite sequence data for Fraxinus excelsior and F. mandschurica
We sequenced the methylomes of twenty ash trees using whole genome bisulphite sequencing (WGBS). These trees consisted of two grafted replicates of Danish Fraxinus excelsior Clone 27, and five grafted replicates each of Danish F. excelsior Clones 33, 35 and 40, two grafted replicates of one F. mandshurica genotype, one sample of another F. mandshurica genotype. Clones 33 and 35 have previously been shown to have consistent low susceptibility to ADB in field trials in Denmark and F. mandshurica is a species with low susceptibility to ADB. DNA was extracted from dried leaves of the twenty samples in December 2013 at the University of Copenhagen, using Qiagen DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and checked for quality and quantity using Nanodrop (Thermo Scientific, Waltham, MA, USA). DNA samples were sent to the Genome Centre at QMUL where they were quantified using Qubit (Invitrogen Carlsbad, CA, United States) and vacuum concentrated. Between 200ng and 500ng of DNA from each sample were bisulphite-converted at the Genome Centre using the EZ DNA Methylation Gold kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using EpiGnome Methyl-seq kit (Epicentre, Madison, WI, USA), and checked on an Agilent Tape Station using the DNA1000 High Sensitivity Screen Tape assay (Agilent, Santa Clara, CA, USA) and sequencing was carried out on an Illumina HiSeq 2500 instrument (Illumina, San Diego, USA), using 2 x 100 bp paired reads. After bisulphite conversion and sequencing, a total of 2,052 million raw reads were generated, with an average of 102.6 million reads (12x coverage) per sample.
We sequenced the methylomes of twenty ash trees using whole genome bisulphite sequencing (WGBS). These trees consisted of two grafted replicates of Danish Fraxinus excelsior Clone 27, and five grafted replicates each of Danish F. excelsior Clones 33, 35 and 40, two grafted replicates of one F. mandshurica genotype, one sample of another F. mandshurica genotype. Clones 33 and 35 have previously been shown to have consistent low susceptibility to ADB in field trials in Denmark and F. mandshurica is a species with low susceptibility to ADB. DNA was extracted from dried leaves of the twenty samples in December 2013 at the University of Copenhagen, using Qiagen DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and checked for quality and quantity using Nanodrop (Thermo Scientific, Waltham, MA, USA). DNA samples were sent to the Genome Centre at QMUL where they were quantified using Qubit (Invitrogen Carlsbad, CA, United States) and vacuum concentrated. Between 200ng and 500ng of DNA from each sample were bisulphite-converted at the Genome Centre using the EZ DNA Methylation Gold kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using EpiGnome Methyl-seq kit (Epicentre, Madison, WI, USA), and checked on an Agilent Tape Station using the DNA1000 High Sensitivity Screen Tape assay (Agilent, Santa Clara, CA, USA) and sequencing was carried out on an Illumina HiSeq 2500 instrument (Illumina, San Diego, USA), using 2 x 100 bp paired reads. After bisulphite conversion and sequencing, a total of 2,052 million raw reads were generated, with an average of 102.6 million reads (12x coverage) per sample.