GSE192670
Analysis of mRNA decay intermediates in Bacillus subtilis 3' exoribonuclease and RNA helicase mutant strains
The Bacillus subtilis genome encodes four 3' exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM.  Previous work showed that PNPase, encoded by the pnpA gene,   is the major 3' exonuclease involved in mRNA turnover; in a pnpA deletion strain, numerous mRNA decay intermediates accumulate.  Whether B. subtilis mRNA decay occurs in the context of a degradosome   complex is controversial.  In this study, global mapping of mRNA decay intermediate 3' ends within coding sequences was performed in strains that were either deleted for, or had an inactivating point mutation in,   the pnpA gene.  The pattern of 3' end accumulation in these strains was highly similar, suggesting that mRNA decay was not occurring in the context of a degradosome, whose structure would be affected by the absence of PNPase.  A comparison with mapped 3' ends in a strain lacking CshA, the major RNA helicase, indicated that different mRNAs may require both PNPase and CshA for efficient decay.  RNA-seq analysis of strains lacking RNase R suggested that this enzyme did not play a major role in mRNA turnover in the wild-type strain.  Strains were constructed that contained only one of the four known 3' exoribonucleases.  When RNase R was the only 3' exonuclease present, it was able to degrade a model mRNA efficiently, showing processive decay even through a strong stem-loop structure that inhibits PNPase processivity.  Strains containing only RNase PH or only YhaM were also insensitive to this RNA secondary structure, suggesting the existence of another, as-yet unidentified, 3' exoribonuclease. Overall design: Term-seq/RNA-seq analysis from Bacillus subtilis strains with pnpA gene deleted or mutated to be catalytically inactive or cshA gene deleted.   Triplicate samples.