ena-STUDY-MPI FOR DEVELOPMENTAL BIOLOGY-18-10-2017-12:54:41:562-272
High contiguity Arabidopsis thaliana genome with a single SMRT cell
We demonstrate a quick and cost effective reference assembly for an Arabidopsis thaliana accession using the Pacific Biosciences Sequel sequencer. We assemble the Arabidopsis accession KBS-Mac-74 into 78 contigs with an N50 length of 10.7 Mb covering 100% (119 Mb) of the non-repetitive genome. We demonstrate that the polished KBS-Mac-74 assembly is highly contiguous with BioNano optical genome maps.
We demonstrate a quick and cost effective reference assembly for an Arabidopsis thaliana accession using the Pacific Biosciences Sequel sequencer. We assemble the Arabidopsis accession KBS-Mac-74 into 78 contigs with an N50 length of 10.7 Mb covering 100% (119 Mb) of the non-repetitive genome. We demonstrate that the polished KBS-Mac-74 assembly is highly contiguous with BioNano optical genome maps.