ena-STUDY-INRA (INRA Jouy en Josas)-04-06-2019-08:24:51:510-565
Exploring Listeria monocytogenes transcriptomes in correlation with divergence of lineages and virulence measured in Galleria mellonella
These genome sequences served for phylogenetic reconstruction and mapping of RNA-Seq reads in a study that examined transcriptome diversity at the species level. As for many opportunistic pathogens, virulence potential of Listeria monocytogenes has been reported to be highly heterogeneous between isolates and correlated, to some extent, with phylogeny and gene repertoires. In sharp contrast with copious data on intraspecies genome diversity, little is known about transcriptome diversity despite the role of complex genetic regulations in pathogenicity. The current study employed RNA sequencing to characterize the transcriptome profiles of 33 isolates under an optimal in vitro growth condition. Transcript levels of conserved single copy genes were comprehensively explored from several perspectives including phylogeny, in silico virulence category based on epidemiological MLST data, and virulence phenotype measured in Galleria mellonella.
These genome sequences served for phylogenetic reconstruction and mapping of RNA-Seq reads in a study that examined transcriptome diversity at the species level. As for many opportunistic pathogens, virulence potential of Listeria monocytogenes has been reported to be highly heterogeneous between isolates and correlated, to some extent, with phylogeny and gene repertoires. In sharp contrast with copious data on intraspecies genome diversity, little is known about transcriptome diversity despite the role of complex genetic regulations in pathogenicity. The current study employed RNA sequencing to characterize the transcriptome profiles of 33 isolates under an optimal in vitro growth condition. Transcript levels of conserved single copy genes were comprehensively explored from several perspectives including phylogeny, in silico virulence category based on epidemiological MLST data, and virulence phenotype measured in Galleria mellonella.