ena-STUDY-Universite-Francois-Rabelais-05-09-2017-10:09:11:932-44
RNA-seq analysis of Catharanthus roseus adventitious roots
Water-induced adventitious roots were generated by immersing freshly cut stems of 6-8 weeks plantlets (Apricot Sunstorm cultivar). Leaves from the last 10 cm of stem were removed before immersion whereas upstream leaves were maintained to allow photosynthesis. Plantlets were placed in greenhouse (28 °C photoperiod) and water was changed every three days. First adventitious roots begin to develop on stems almost five days after immersion and reach a 5-cm length around two weeks later. Apical parts of the roots (1cm) were harvested for RNA sequencing.RNA-seq library preparation and high-throughput sequencing were performed by Eurofins using Illumina HiSeq 2000/2500 with chemistry v3.0 in the paired mode.
Water-induced adventitious roots were generated by immersing freshly cut stems of 6-8 weeks plantlets (Apricot Sunstorm cultivar). Leaves from the last 10 cm of stem were removed before immersion whereas upstream leaves were maintained to allow photosynthesis. Plantlets were placed in greenhouse (28 °C photoperiod) and water was changed every three days. First adventitious roots begin to develop on stems almost five days after immersion and reach a 5-cm length around two weeks later. Apical parts of the roots (1cm) were harvested for RNA sequencing.RNA-seq library preparation and high-throughput sequencing were performed by Eurofins using Illumina HiSeq 2000/2500 with chemistry v3.0 in the paired mode.