PRJNA861325
RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification
Neural crest development is transcriptionally controlled via sequential activation of gene regulatory networks (GRNs). Recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using available single cell RNA-sequencing data from avian embryos to identify potential post-transcriptional regulators, we identified in premigratory cranial neural crest enrichment of Elavl1, which encodes for an RNA-binding protein with roles in transcript stability. Perturbation of Elavl1 resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN, phenotypes also observed with downregulation of the canonical Wnt inhibitor Draxin. RNA-sequencing, RNA-immunoprecipitation, and RNA decay and proximity ligation assays further showed that Draxin is the primary target for stabilization by Elavl1 during cranial neural crest specification, and downregulation of neural crest specifier expression with Elavl1 knockdown was largely due to loss of Draxin. Importantly, overexpression of exogenous Draxin rescued cranial neural crest specification defects observed with Elavl1 knockdown. Thus, Elavl1 plays a critical a role in the maintenance of cranial neural crest specification via Draxin mRNA stabilization. Together, these data highlight an important intersection of post-transcriptional regulation with modulation of the neural crest specification GRN.