Developmental_and_molecular_heterogeneity_of_mouse_hematopoietic_stem_cells-sc-2840
Developmental_and_molecular_heterogeneity_of_mouse_hematopoietic_stem_cells
This study aims to investigate the developmental and molecular heterogeneity of mouse hematopoietic stem cell (mHSC). The genome-wide transcriptomic heterogeneity in wildtype and Bcl11a deficient mHSC will be stuided by single-cell mRNA sequencing technology.   Brief protocol: Adult mouse LSK HSC is harvested from femur and FACS purified by surface markers CD48-, CD150+. Single cell capture and cDNA preparation are performed by Fluidigm C1 microfluidic system according to manufacturer protocol. Library is prepared by Illumina Nextera XT kit.
This study aims to investigate the developmental and molecular heterogeneity of mouse hematopoietic stem cell (mHSC). The genome-wide transcriptomic heterogeneity in wildtype and Bcl11a deficient mHSC will be stuided by single-cell mRNA sequencing technology.