E_coli_MG1655_Prokaryotic_RNA_Seq
Escherichia coli str. K-12 substr. MG1655 Transcriptome or Gene expression
The goal was to establish a robust and scalable RNA-seq process applicable to cultured bacteria as well as to complex community transcriptomes.  To this end, we evaluated rRNA depletion methods and chose a protocol that eliminates rRNA reads efficiently and robustly, and largely irrespective of the quality of the RNA input sample.