ena-STUDY-EESC/USP-17-10-2013-20:55:22:249-139
For evaluated of biomass adaptation, two EGSB reactors were operated. RAB (adapted biomass) reactor was operated in two stages: Stage I, with standard Linear Alklybenzene Sulfonate - LAS (technical grade; 13.2 mg.L-1); Stage II, the standard LAS, was replaced by diluted laundry wastewater according to LAS concentration (11.2 mg.L-1). RNAB (not adapted biomass) reactor had single stage (Stage I), directly with the wastewater (11.5 mgLAS.L-1). Thus, the 16S rRNA pyrosequencing was performed for two samples from sludge blanket: (i) Stage II - RAB and (ii)Stage I - RNAB. The samples were sequenced in a 454 platform. The two samples were sequenced in a run using barcodes. One sff file contain the reads from all samples.
For evaluated of biomass adaptation, two EGSB reactors were operated. RAB (adapted biomass) reactor was operated in two stages: Stage I, with standard LAS (technical grade; 13.2 mg.L-1); Stage II, the standard LAS, was replaced by diluted laundry wastewater according to LAS concentration (11.2 mg.L-1). RNAB (not adapted biomass) reactor had single stage (Stage I), directly with the wastewater (11.5 mgLAS.L-1). Thus, the strategy of biomass adaptation did not result in an increase of surfactant removal present in wastewater (RAB Stage II: 77%; RNAB Stage I: 78%). PCR-DGGE revealed 80% similarity in the phases with laundry wastewater (sludge bed) despite the different strategies starting of reactors. Moreover, pyrosequencing showed many reads close to genera related to the degradation of aromatic compounds (as LAS) and sulfate reduction (Desulfobulbus and Syntrophorhabdus). Probably, the insignificant difference in LAS removal between two strategies adopted was due to the great microbial richness of inoculum.
For evaluated of biomass adaptation, two EGSB reactors were operated. RAB (adapted biomass) reactor was operated in two stages: Stage I, with standard LAS (technical grade; 13.2 mg.L-1); Stage II, the standard LAS, was replaced by diluted laundry wastewater according to LAS concentration (11.2 mg.L-1). RNAB (not adapted biomass) reactor had single stage (Stage I), directly with the wastewater (11.5 mgLAS.L-1). Thus, the strategy of biomass adaptation did not result in an increase of surfactant removal present in wastewater (RAB Stage II: 77%; RNAB Stage I: 78%). PCR-DGGE revealed 80% similarity in the phases with laundry wastewater (sludge bed) despite the different strategies starting of reactors. Moreover, pyrosequencing showed many reads close to genera related to the degradation of aromatic compounds (as LAS) and sulfate reduction (Desulfobulbus and Syntrophorhabdus). Probably, the insignificant difference in LAS removal between two strategies adopted was due to the great microbial richness of inoculum.