DRP000321
Eptatretus burgeri strain:riken_grau-0002 Genome sequencing
The brain was obtained from the adult specimens of the E. burgeri which were anesthetized by MS-222. The obtained tissues were frozen in liquid nitrogen, ground to a fine powder, and homogenized in TRIzol reagent (Invitrogen). Total RNA extraction was performed according to the provided protocol (Invitrogen). The extracted RNA sample was used for 454 library construction and sequencing, following protocols (Margulies et al., 2005).