GSE133458
iTag labelling of Liver, Plasma and VsWAT RNA
Using a novel method for metabolic labelling of RNA called iTag RNA we isolate hepatocytes' and kidney proximal epithelial cells' transcriptional response to a dietary challenge and identify multiple hepatocyte-secreted small ccfRNAs in plasma, and transfer of these hepatic derived ccfRNAs to VsWAT Overall design: mRNA /small RNA sequencing from liver plasma and kidney of male 12 week old mice following 2w of high fat diet feeding or low fat diet control