GSE169564
Tn-seq of hydroxyurea treated Bacillus subtilis cells
Hydroxyurea (HU) is thought to primarily target ribonucleotide reductase  (RNR), therefore inhibiting the conversion of rNTPs into dNTPs and slowing DNA replication. To understand how Bacillus subtilis responds to HU stress,  we performed RNA-seq and Tn-seq. We obtained genes with fitness defects following hydroxyurea treatment over 3 growth periods. Overall design: A 120,000 insertion transposon library in PY79 was challened with vehicle control (water) or hydroxyurea for 3 growth periods to find genes important in HU-stress recovery