PRJNA866680
African swine fever virus strain:Asfavirus Raw sequence reads
Transfection and infection procedures were used to create ASFVs with different gene deletions generated by homologous recombination between the ASFV-GZ virus and recombination transfer vectors, designated as ASFV-GZdeltaI177LCD2vMGF (including the five genes encoding the corresponding proteins of I177L, CD2v, MGF360-12L, MGF360-13L, and MGF360-14L), respectively. We purified the gene-deleted mutants to homogeneity by successive plaque assays and limiting dilution purification processes.