GSE99153
RNA-seq transcriptional profiling of wildtype and Pax5 knockout mouse pro-B cells
Total RNA was isolated from wild type and Pax5-/- pro-B cells. Samples were sequenced on an Illumina Hiseq 2000 at the Beijing Genomics Institute to produce 90bp paired-end reads. Reads were aligned to the mm10 genome using Subread with unique alignment. The number of read pairs mapped to each gene in the NCBI RefSeq annotation was counted using the featureCounts function of the Rsubread package. Overall design: Two independent Pax5-/- samples were prepared and one wild type.