A. thaliana root small RNA sequencing
High-resolution profiling of small RNAs in the Arabidopsis thaliana root
Columbia-0 ecotype. Cell types were isolated using fluorescence activated cell sorting as described in Birnbaum et al. (2003), and developmental zones were hand dissected.  More details in library construction protocols.  Associated publication is Breakfield, Corcoran, et al. (2011) High-resolution experimental and computational profiling of tissue-specific known and novel miRNAs in Arabidopsis. Genome Research (accepted).
Small noncoding RNAs (ncRNAs) are key regulators of plant development through modulation of the processing, stability and translation of larger RNAs.  In this project we present small RNA datasets produced from over 200 million Illumina sequencing reads covering all major cell types of the root (epidermis, cortex, endodermis, vascular tissue or stele, and columella) as well as four distinct developmental zones (early meristematic, late meristematic, elongation, early differentiation).