GSE172292
Shp1 protein tyrosine phosphatase function in the early development of the hematopoietic system of zebrafish
We developed a ptpn6 knock-out zebrafish and found a disruption in the early development of the hematopoietic system. Marcrophage subpopulation size en numbers of macrophage progenitors differ between mutants and siblings. Overall design: 5dpf zebrafish embryos were dissociated and FACS sorted. Macrophages (mpeg) were sorted in 384 well plates.