PRJNA453196
RNAseq for PRC1 complex
Lifespan analysis demonstrated that animals deficient in scec244 lived longer than WT, coupled with a reduction of ubH2A. Remarkably, double mutants demonstrated more striking effects on life-extension and ubH2A-reduction. This evidence indicates that reducing ubH2A promotes adult longevity. Since biological roles of histone modification link chromatin accessibility to gene expression31,32, we speculated that alterations in the levels of ubH2A could impact the transcriptome. We then asked whether transcriptional change of particular genes might account for ubH2A-dependent regulation on aging. Using dissected head tissues, we generated RNA-seq datasets for polyA-selected mRNAs on WT and Scec244, Su(Z)2c433 trans-heterozygous double mutants that gave rise to the stronger life-extension phenotype than single mutants.