GSE161613
Next Generation Sequencing  Analysis of Wild Type and Chd8-/- LSK in Transcriptomes, Chromatin accessibility and chromatin state mapping
Next-generation sequencing (NGS) has been used for study the transcriptomic and genomic change after Chd8 dletion. The goals of this study are to compare NGS-derived LSK transcriptome profiling (RNA-seq), ATAC and H3K4me3 and H3K27me3 Cut&Run data to find downstream targets of CHD8 and its impact on chromatin state. Overall design: LSK cells were sorted by flow at 6 days after CHD8 deletion induction, then preceed to RNA extraction, Tn5 Transposase digestion and H3K4me3, H3K27me3 Cut&Run assay.