GSE112228
Conserved miRNA discovery in switchgrass
Small RNA libraries were constructed, sequenced, and analyzed with our Sequence Homology Pipeline for miRNA discovery (Jeong et al. 2013) to identify 59 unique conserved miRNA sequences from 199 precursors in switchgrass. Overall design: Eight samples in total were analyzed. Three were tissue samples. The remaining 5 samples included drought and cold stress conditions along with controls.  Switchgrass  (AP13)  was  grown  under  greenhouse  conditions  with  an  average  day  temperature  of  26°C  and  an  average  night  temperature  of  20°C.    Stress  treatments  were  carried  out  two  months  after  propagation  from  cuttings  and  the  second  and  third  leaf  blades  from  the  top  were  sampled. Cold  treatments  at  4°C  were  performed  for  24  hours.  For  drought  experiments,  the  drought  sample  was  harvested  11  days  after  watering  was  stopped.  Plants  subjected  to  recovery  were  then  watered,  and  on  the  12th  day  they  were  sampled.