GSE131895
RNA analysis of Nr4a1 and Nr4a1-knockout mammary tumor cells from MMTV-PyVT transgenic mice
MMTV-PyVT Mammary tumors were harvested from PyVT-wild-type (WT), PyVT-Nr4a1 knockout (KO)  littermates on a congenic FVB/NJ background,minced and incubated with collagenase type I digestion solution .The digested Mammary tumor cells were collected and maintained in DMEM medium containing 10% FBS for 24 hour. Overall design: Differential mRNA expression of Mammary tumor cells between PyVT-wild-type (WT) mice and age matched PyVT-Nr4a1 knockout (KO) mice was quantified by RNA-seq data analysis