GSE97865
The 19S proteasome subunit Rpt4 is directly involved in the regulation of heterochromatin spreading
The non-proteolytic role of the proteasome in heterochromatin regulation Overall design: For ChIP experiment : ChIP against H3K9me2, anti-FLAG was performed for a wild type strain and strains lacking Clr4 and FLAG-tagged Rpn1 and Pre1. For sRNA experiment : Total RNA was prepared from cells arrested in mid-log phase followed by sRNA isolation.