GSE209715
Chromatin structure and gene expression signatures in FUS low and high expressed hematopoietic stem cells
Aged hematopoietic stem cells (HSCs) exhibit compromised reconstitution capacity. In this study, we observed that the expression of FUS is increased in aged HSCs. We aimed to analysis the changes of chromatin structure  and  gene expression signatures in FUS low and FUS high  expressed hematopoietic stem cell (lineage-, c-kit+ Sca-1+ CD34-) using tagHi-C and RNA-seq .We also conducted  CTCF CUT&RUN experiment in Young(2 months) and Aged(30 months) hematopoietic stem cell (lineage-, c-kit+ Sca-1+ CD34-)  to analysis  CTCF signal changes. These hematopoietic stem cell (HSC) were purified from the bone marrow of mice with fus-gtp or different ages. Results provide insight how aberrant FUS mobility effects chromatin structure and promotes HSC aging . Overall design: We compare chromatin structure  and gene expression signatures using Hi-C, RNA-seq, CTCF CUT&RUN in Fus low, Fus high, Aged, Young HSCs from the bone marrow of mice.