GSE138805
Transcriptomic changes of mouse monocytes and macrophages after ischemic stroke
Blood monocytes/macrophages infiltrate the brain after ischemic stroke and critically influence brain injury and regeneration. We investigated stroke-induced transcriptomic changes of monocytes/macrophages by RNA sequencing profiling, using a mouse model of permanent focal cerebral ischemia. Compared to non-ischemic conditions, brain ischemia induced only moderate genomic changes in blood monocytes, but triggered robust genomic reprogramming in monocytes/macrophages invading the brain. Surprisingly, functional enrichment analysis of the transcriptome of brain macrophages revealed significant overrepresentation of biological processes linked to neurovascular remodeling, such as angiogenesis and axonal regeneration, as early as 5 days after stroke, suggesting a previously underappreciated role for macrophages in initiating post-stroke brain repair. Upstream Regulator analysis predicted peroxisome proliferator-activated receptor gamma (PPAR?) as a master regulator driving the transcriptional reprogramming in post-stroke brain macrophages. Importantly, myeloid cell-specific PPAR? knockout (mKO) mice demonstrated lower post-stroke angiogenesis and neurogenesis than wild-type mice, which correlated significantly with the exacerbation of post-stroke neurological deficits in mKO mice. Collectively, our findings reveal a novel repair-enhancing transcriptome in brain macrophages during post-stroke neurovascular remodeling. As a master switch controlling genomic reprogramming, PPAR? is a rational therapeutic target for promoting and maintaining beneficial macrophage functions, facilitating neurorestoration, and improving long-term functional recovery after ischemic stroke. Overall design: C57BL/6J mice (9 weeks old, male) were subjected to focal cerebral ischemia induced by permanent occlusion of the left distal middle cerebral artery and left common carotid artery. The CD11b+CD45high cell population was collected by fluorescence-activated cell sorting from the blood after sham operation, and from the blood and brain after ischemic stroke. There were 3 groups: sham blood, stroke blood, and stroke brain. In each group there were 3 biological replicates. For blood samples, each replicate was from one mouse. For brain samples, each replicate was pooled from 5 mouse brains. The sorted cells were subjected to mRNA sequencing.