GSE193137
Neonatal murine cardiomyocyte transcriptome
Cardiomyocyte poly(A) RNA was sequenced from purified bulk cardiomyocytes collected from one male and female murine heart at postnatal day 2 (P2).  Neonatal cardiomyocytes were isolated and purified (96% cardiomyocytes at P2) by Langendorff retrograde perfusion and immunomagnetic cell separation, respectively. We found evidence of sexual dimorphism with 9 differentially expressed genes (FDR<0.05) encoded on XY chromosomes in this RNA-Seq dataset. Overall design: Cardiomyocytes were collected from one male and female heart from 4 different litters (n=4 per biological sex) of an average C57BL6/J litter size of 6-8 mouse pups.