E-MTAB-6265
In situ depletion of Nuak1 in murine colon tumours
The aim of the project is to determine the impact of NUAK1 depletion on the transcriptome of in situ colon tumours.  Tumours were initiated in Villin-CRE-ER;APC-floxed;DI-shNUAK1 mice, followed by treatment for 1 week with dextran sodium sulphate.  68 days after tumour initiation, NUAK1 shRNAs were induced by doxycycline administration (via gavage), or left un-induced.  6 tumours were harvested from NUAK1 shRNA expressing mice and 6 from controls.  RNA was isolated using Qiagen RNEasy and analyzed by Illumina paired-end RNA-SEQ
The aim of the project is to determine the impact of NUAK1 depletion on the transcriptome of in situ colon tumours.  Tumours were initiated in Villin-CRE-ER;APC-floxed;DI-shNUAK1 mice, followed by treatment for 1 week with dextran sodium sulphate.  68 days after tumour initiation, NUAK1 shRNAs were induced by doxycycline administration (via gavage), or left un-induced.  6 tumours were harvested from NUAK1 shRNA expressing mice and 6 from controls.  RNA was isolated using Qiagen RNEasy and analyzed by Illumina paired-end RNA-SEQ