PRJNA865458
Effect of sodium nitrate and cysteamine on rumen fermentation, amino acid metabolism and microbiota in buffalo in vitro
Illumina MiSeq sequencing was carried out after librar preparation from purified DNA using primers for V3-V4 region of 16S rRNA-gene. DNA libraries were sequenced using a 2x300 . DNA samples from two levels of methionine (0.28%, and 1.12%) were selected for sequencing of 16S rRNA gene to determine bacterial diversit .