PRJNA718971
DNA methylome analysis in Pigeonpea at single base resolution
In this study, two pigeonpea 11A and 303R lines were crossed and the resulting F1 hybrid were subjected to bisulfite sequencing for methylome characterization at single base resolution. A total of six bud samples were collected in liquid nitrogen and then stored at -80C and Total genomic DNA was isolated from frozen bud samples by using CTAB protocol. The quality and concentration of DNA samples were measured using Qubit and Agilent 2100 Bioanlyzer. The libraries were sequenced in pair end mode by Illumina HiSeq XTen System.