GSE137165
Single Cell RNAseq profiles of thymic ETP populations
The single cell profiles of sorted ETP populations with cell hashing antibody barcoding from mouse thymus Overall design: Examination of single cell expression profile of sorted populations identified from previous pseudotime analysis. Experiment was performed using droplet-based 3' end RNA expression profiling using Chromium Single Cell 3' Reagent Kits v3 according to manufacturer's protocol (10x Genomics), with the FACS sorted individual sub-populations of ETPs tagged with Totalseq-A hashtags (Biolegend).  The cDNA library was sequenced on Illumina Novaseq for 400 million reads, and tag library on HiSeq4000 for 20 million reads, both in pair-end mode following manufacturer's instructions. Please note that meta.csv.gz contains assigned sample names of individual cells after demultiplexing