PRJNA513975
Furlbach diversity
In this study, we compared the efficiency of different methods of species delimitation by analyzing a community of nematodes from a reference stream of good water quality. Specifically, we compared the performances of traditional microscopy, single-specimen barcoding (Sanger sequencing) and metabarcoding to identify 500 nematodes for each method in sediment samples. The molecular approaches analyzed two gene regions, the ribosomal large and small subunits (LSU and SSU).