GSE138750
Transcriptomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase
Overexpression of the protein phosphatase Ppz1 is toxic for yeast cells as it blocks cell cycle progression from G1 to S phase. To identify relevant changes caused by Ppz1 overexpression we have constructed a strain (ZCZ01) in wich the PPZ1 promoter has been replaced by the GAL promoter. Cell grown on raffinose were shifted to galactose and samples were taken after 30, 120 and 240  min and changes in mRNA levels analyzed by RNA-seq. Overall design: Six different samples were analyzed. Three were controls (wild type strain) taken 30, 120 and 240 min after shifting cells to galactose, and three were the equivalent samples from the ZCZ01 (GAL1:PPZ1) strain.