GSE159006
An early cortical progenitor-specific mechanism regulates thalamocortical innervation
Comparison of the wild-type subplate transcriptome with that of the Emx1Cre11.5;Lhx2lox/lox and NexCre;Lhx2lox/lox subplate and further analysis of differentially expressed genes that were specific to the Emx1Cre11.5;Lhx2lox/lox dataset Overall design: Microdissected tissue from 2 postnatal day zero (P0) brains was pooled to make one replicate sample. Two samples each of control, Emx1Cre11.5;Lhx2lox/lox and NexCre;Lhx2lox/lox were processed for RNA sequencing after ascertaining that their RIN values were >8. RNA sequencing was performed by Medgenome Technologies, Bangalore. mRNA was isolated from total RNA, cDNA was prepared, and libraries were prepared using the IlluminaTruSeq RNA Sample preparation V2. Libraries were multiplexed and sequenced as 100 base-pairs (bp) pair-end reads using the Illumina HiSEQ2500 platform, at an expected depth of 60M single reads.