GSE125504
RNA-Seq analysis of human Tr1, Tregs and IL10neg cells
To determine the differentially expressed genes between these subsets and identify the core human in vivo differentiated Tr1 cell gene signature Overall design: Human Tr1 and IL-10 neg cells were sorted using an IL-10capture assay following 16h CD3/CD28 bead activation, matched Tregs were sorted from unstimulated cells then activated by CD3/CD28 beads for 16h.  Samples are from 3 donors from 3 independent experiments (HIF037, HIF038 and HIF039).