GSE202900
Synonymous codon usage regulates translation initiation
Ribo-seq with firefly luciferase optimality reporters to determine whether incomplete translation and/or inhibited translation initiation is responsible for the reduced ribosome occupancy on nonoptimal transcripts. Overall design: Performed Ribo-seq, with paired RNA-seq, on HEK293T cells transfected with either optimal or nonoptimal reporters. Experiments were performed in triplicate.