GSE178530
Deep sequencing data from PFS screen of SuCas12a2 CRISPR-Cas nuclease
In this work we analyzed PFS sequence recognized by SuCas12a2 Type V CRISPR-Cas nuclease Overall design: SuCas12a2 was challanged with a library of randomized flanking sequences. Two biological replicates were prepared and analyzed. Each replicate consisted of a library of flankign sequences challanged by SuCas12a2 (pOD4) and a library processed in the absence of SuCas12a2 (pOD49). Sequences were extracted from the Illumina data and their corresponding depletion scores were calculated.