Primordial_germ_cell_bisulphite_libraries_____-sc-2013-03-15T10:38:23Z-2568
Primordial_germ_cell_bisulphite_libraries_____
In this study we aim to assess the methylation and hydroxymethylation profile in mouse primordial germ cells from control and Thymine DNA Glycosylase (TDG)-deleted samples by bisulfite-sequencing of DNA extracted from primordial germ cells at different time points between embryonic day 9.5 and embryonic day 12.5.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We will compare bisulphite libraries from primordial germ cells (PGCs) from Thymine DNA-glycosylase knockout and control embryos in order to characterise the effect of TDG deletion on the methylation and hydroxymethylation profiles of PGCs.We will conduct bisulfite-sequencing of DNA extracted from primordial germ cells from different time points, between embryonic day 9.5 and embryonic day 12.5.