GSE201992
HIRA loss transforms FH deficient cells [RNA-Seq]
Purpose: Identifying transcriptional changes led by Hira loss in FH deficient cells Methods: RNA was extracted from the cell lines using QIAGEN kit folloging the manufacturer instructions. RNA concentrator kit was used to guarantee RNA quality. Results/Conclusion: We identified MYC/E2F1 signatures as the top upregulated ones controlling the oncogenic events occuring in Fh1 and Hira deficient cells. Overall design: Transcriptional study of Fh1 proficient cells (Fh1 fl/fl), Fh1 deficient cells (Fh1-/-) and Hira loss through the use of a gRNA (Fh1 fl/fl g1Hira and Fh1-/- g1Hira)