ena-STUDY-University Clermont Auvergne-13-08-2021-09:40:44:683-1085
The colonisation of Arabidopsis thaliana by Streptomyces spp. is impacted in a pleiotropic mutant chs5
We have compared the interaction of Arabidospsis thaliana Col-0 (wild-type) or a isoprenoid deficient biosynthetic mutant chs5, having a pleiotropic metabolic impair, with the bacterial pathogen Pseudomonas syringae pv. tomato DC300 or with its microbiota present in disctinct compartments (roots, rhizosphere, phyllosphere. Microbiota analysis was performed using 16S rDNA metabarcoding. In order to get further affiliation of OTUs of interest, gene capture by hybridization targeting the 16S and 18S rDNA was performed on a pool of DNA extracted from rhizosphere of WT plants.
We have compared the interaction of Arabidospsis thaliana Col-0 (wild-type) or a isoprenoid deficient biosynthetic mutant chs5, having a pleiotropic metabolic impair, with the bacterial pathogen Pseudomonas syringae pv. tomato DC300 or with its microbiota present in disctinct compartments (roots, rhizosphere, phyllosphere. Microbiota analysis was performed using 16S rDNA metabarcoding. In order to get further affiliation of OTUs of interest, gene capture by hybridization targeting the 16S and 18S rDNA was performed on a pool of DNA extracted from rhizosphere of WT plants.