GSE161638
Next generation sequencing of chicken otocyst with pharmacologicaly induced loss-of-Wnt signalling
Bulk RNA-sequencing of individual E2.5 chicken otocyst incubated in media enriched with Wnt signalling inhibitor IWR-1 or control condition (DMSO) for 24h. Transcripts abundance was mapped to a chicken reference genome using Kallisto package and differentially expressed genes were identify using Sleuth package (genes with p-value < 0.05 were considered as significant). Biological Function enrichment analysis (Toppgene online tool) showed that many of these genes were associated with neurogenesis. Overall design: Identification of genes deregulated in E3 otic explants treated with IRW-1 or DMSO for 24h