GSE204671
Identification of slow-cycling cells in Ewing sarcoma
We identified slow-cycling cells (SCCs) in Ewing sarcoma using a label retention assay with CFSE. We labeled cells of SK-ES-1, an Ewing sarcoma cell line, with CFSE. After 5 days culture, we isolated cells retaining strong fluorescence (upper, ~10%) as SCCs and other cells (lower, ~90%) as non-slow-cycling cells (non-SCCs) using FACS AriaTM ? cell sorter. Overall design: Comparative gene expression profiling analysis of RNA-seq data for SCCs and non-SCCs.