GSE132507
ON and OFF SACs identified by scRNA-seq
The goal of this experiment was to investigate the heterogeneity of SACs via scRNA-seq Overall design: P0 ChATCre:Thy1-STP-YFP (line#15) retinas were digested with papain (Worthington, LS003126) at 37°C for 10 mins. Retinas were then dissociated and triturated into single cell suspensions. YFP labeled SACs were further isolated by FACS. Single SAC suspensions were loaded into 10X Chromium Single Cell A Chips at a concentration of ~1,000 cells/µL. Single cell libraries were prepared using the Chromium 3' v2 platform (10X Genomics, Pleasanton, CA) as previously described (Peng et al., 2019). Libraries were sequenced on the Illumina HiSeq 2500 (Paired-end reads: Read 1, 26bp, Read 2, 98bp).