PRJNA707404
A versatile reverse genetics platform for pandemic SARS-CoV-2 and other positive-strand RNA viruses.
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA could be readily assembled in vitro by a novel circular polymerase extension reaction (CPER) methodology without the need for technically demanding intermediate steps.