GSE211364
Whole transcriptome profiling of glioma tissue with different treatment
To test stiffness induced alternative splicing, GBM stem cells were cultured on different stiffness gels (500Pa versus 5k Pa) for 48h before RNA extraction. To test PIEZO1-knockdown induced alternative splicing, GBM stem cells were cultured on 5K Pa stiffness gel and treated by PIEZO1 shRNA for 72 hours before collecting total RNA. Overall design: Explore the stiffness induced alternative splicing.