PRJNA794328
Probabilistic modeling of ambient noise for multiple single-cell omics technologies
We conducted this CROP-seq study to evaluate the performance of scAR. We designed a viral pool of 99 sgRNAs targeting 13 different genes, most of them being essential in MCF7 cells. We infected MCF7 cells expressing dCas9-KRAB with the lentiviral libraries at extraordinary low multiplicity of infection (MOI=0.3) to ensure single integrations of sgRNAs. Cells were harvested at D5 or D12 post-transduction.