GSE112450
Polycomb repressive complex 1 (PRC1) role in skin epithelium development [ChIP-seq Auts2, Bmi1, Rybp]
Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes.  Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification. Overall design: Newborn mouse epidermal progenitor cells  were FACS purified. Immunoprecipitated chromatin was analyzed by ChIP-seq.