GSE125854
Analysis of seeds from Aethionema arabicum Turkish (TUR) and Cyprus (CYP) accession in response to light
The seed germination in response to light shows natural variation in two accessions of Aethionema arabicum (Brassicaceae). One accession from Turkey (TUR) germinates well in darkness and light, while another closely related accession from Cyprus (CYP) germinates only in the dark. To understand the transcriptional differences between the two accession, we performed RNA-seq analysis from imbibed seeds, which were kept either in darkness or under light for 23 hours. Results provide the first transcriptional comparison from light-neutral (TUR) and light-sensivite (CYP) seeds kept in darkness or exposed to light. Overall design: Imbibed seeds of TUR and CYP seeds were kept in darkness or exposed to white light (100 µmol m-2s-1) for 23 hours. mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. In total, 12 samples were analyzed (2 accessions x 2 conditions x 3 repetitions)