Targeted_sequencing_of_OE_tumours_and_surrounding_areas-sc-4733
Targeted_sequencing_of_OE_tumours_and_surrounding_areas
We would like to deep target sequence mouse oesophageal epithelial tumours, and areas surrounding these tumours, generated using a chemical carcinogenesis protocol (DEN and DEN+SOR). The background is C57Bl/6. These samples are in triplicates and have already been whole exome sequenced. We have samples per animal and we have liver and ear DNA samples as a controls for every animal which should function as a reference genome.
We have extensively characterised the clonal expansion and tumours in the mouse oesophagus by genetic lineage tracing, immunofluorescence and gene expression array analysis. We would like now to investigate the genotype of lessions and surrounding areas to find which pathways are mutated that promote tumour initiation.