PRJNA491850
Sequencing of FFPE autopsy samples from West Nile infected human brains
The goal of this experiment is to identify the differential expressed genes in cerebellum of West Nile virus infected brain samples. Total RNAs were extracted from cerebellum FFPE autopsy samples from West Nile infected or normal human brain. Illumina RNA libraries were made from isolated total RNA according to the Illumina mRNA-seq sample preparation kit (Illumina, San Diego, CA, USA) eliminating the poly-A selection and RNA fragmentation steps. Libraries were sequenced on GAIIx machine with a single-end run.