GSE184214
SMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression [BSA_0275_SMNDC1]
We have found that loss of SMNDC1 (Survival Motor Neuron Domain Containing 1), a component of the spliceosome complex, in both murine alpha cells and human islets is capable of inducing an overall increase in beta cell markers and activating insulin transcription. Overall design: Paired-end RNAseq results of alphaTC1 cells (murine alpha cell line) 5 days after transduction with an Smndc1 shRNA. Additionally, to further study the subpopulation of alpha cells that robustly induce insulin expression, we co-transduced the cells with a Rat Insulin Promoter and sorted for the positive and negative populations post-knockdown.