GSE135789
Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche (RNA-Seq)
Macrophages are strongly adapted to their tissue of residence. Yet, we know little about the cell-cell interactions that imprint the tissue-specific identities of macrophages in their respective niches. Using conditional depletion of liver Kupffer cells, we traced the developmental stages of monocytes differentiating into Kupffer cells and mapped the cellular interactions imprinting the Kupffer cell identity. Kupffer cell loss induced the tumor necrosis factor (TNF) and interleukin-1 (IL-1) receptor-dependent activation of stellate cells and endothelial cells, resulting in the transient production of chemokines and adhesion molecules orchestrating monocyte engraftment. Engrafted circulating monocytes transmigrated into the perisinusoidal space, and acquired the liver-associated transcription factors ID3 and LXRa. Coordinated interactions with hepatocytes induced ID3 expression, while endothelial cells and stellate cells induced LXRa via a synergistic NOTCH-BMP pathway. This study shows that the Kupffer cell niche is composed of stellate cells, hepatocytes and endothelial cells that together imprint the liver-specific macrophage identity. Overall design: Microarray = different time points during development of Kupffer cells during embryogenesis and after depletion. This data was also compared to other tissue resident macrophages from the Immgen Consortium Database (GSE15907 and GSE37448). Bulk RNA Seq = Hepatic Stellate Cells (HSCs), Liver Sinusoidal Endothelial Cells (LSECs) and hepatocytes. For each cell type there are 4 replicates from Pbs mice, 12h after DT-injection mice and 36h after DT-injection mice.