ena-STUDY-UNIVERSITY OF MONTPELLIER 2-18-10-2017-19:43:02:530-32
The present project encompass sequencing one chicken (Gallus gallus) sample enrich sheared genomic DNA in its GC-rich fraction by subtracting AT-rich DNA.
Standard Illumina libraries are biased towards sequences of intermediate GC-content. This results in an underrepresentation of GC-rich regions in sequencing projects of genomes with heterogeneous base composition, such as mammals and birds. The present project encompass sequencing one chicken (Gallus gallus) sample enrich sheared genomic DNA in its GC-rich fraction by subtracting AT-rich DNA. This was achieved by heating DNA up to 90°C before applying Illumina library preparation.
Standard Illumina libraries are biased towards sequences of intermediate GC-content. This results in an underrepresentation of GC-rich regions in sequencing projects of genomes with heterogeneous base composition, such as mammals and birds. The present project encompass sequencing one chicken (Gallus gallus) sample enrich sheared genomic DNA in its GC-rich fraction by subtracting AT-rich DNA. This was achieved by heating DNA up to 90°C before applying Illumina library preparation.