E-MTAB-4178
RNA-seq analysis of mouse (C57/B6) small intestinal tissue after Porcupine Inhibitor treatment or vehicle control.
Mice were treated with 5 mg/kg LGK974 (Porcupine Inhibitor) for 4.5 days (BID, p.o.). Whole tissue (about 1cm length) from the proximal small intestine was harvested and used for RNA purification. The RNA Integrity was analysed with a NanoChip (Agilent RNA 6000). A total of 2ug of RNA per sample was purified via Poly-A selection and sequenced. Libraries were prepared according to the Illumina TruSeq protocol and sequenced on an Illumina GAIIx. Data was aligned to Mus_musculus.GRCm38.75 library.
Mice were treated with 5 mg/kg LGK974 (Porcupine Inhibitor) for 4.5 days (BID, p.o.). Whole tissue (about 1cm length) from the proximal small intestine was harvested and used for RNA purification. The RNA Integrity was analysed with a NanoChip (Agilent RNA 6000). A total of 2ug of RNA per sample was purified via Poly-A selection and sequenced. Libraries were prepared according to the Illumina TruSeq protocol and sequenced on an Illumina GAIIx. Data was aligned to Mus_musculus.GRCm38.75 library.