GSE123856
A non-catalytic function of carbonic anhydrase IX contributes to the glycolytic phenotype and pH regulation in human breast cancer cells
We have hypothesized that carbonic anhydrases (CAs) play a role in stabilizing both intracellular and extracellular pH to a value that favors cancer progression and metastasis. This is likely in cooperation with the ion transport family.  Here, we examined the expression pattern of selected ion transporters, glucose transporters, and carbonic anhydrases in a triple negative cell model of human breast cancer (UFH-001) and the effect of hypoxia.  Heat map analysis of RNA transcripts from normoxic and hypoxic cells were significantly different.  Additionally, our results show that transcription of the MCT1 monocarboxylate transporter, but not that of MCT4, was sensitive to hypoxia.  Both MCT1 and MCT4 were robustly expressed at the protein level, but neither were significantly upregulated by hypoxia. Only low levels of the vATPase and NHE1 transcripts were detected, which is consistent with little to no detectable signal at the protein level. Our data also show high transcript levels of the GLUT1-glucose transporter, CAII and CAIX.  Only GLUT1 and CAIX transcription were significantly increased by exposure to hypoxia.  CAXII transcripts levels were very low, confirming the lack of expression at the protein level.  Yet, CAXII transcription was significantly increased by hypoxia.  Overall protein levels of our proteins of interest matched transcription patterns.  These studies show a coordinate expression at both the transcriptional and translational levels of the monocarboxylate transporters, the GLUT1 transporter and CAIX in the aggressive triple negative UFH-001 breast cancer cells. Overall design: Examination of UFH-001 Triple Negative Breast Cancer cell line under normoxic and hypoxic conditions