ena-STUDY-The James Hutton Institute-05-01-2018-10:47:50:772-752
Myzus cerasi clonal line contig genome assembly
Myzus cerasi is a relative of Myzus persicae, they differ hugely in their host range. We sequenced the genome of M. cerasi and compared this to other available aphid genomes, including M. persicae. We found that relatively little synteny has been conserved between M. cerasi and M. persicae (when compared to other aphids) and their effector repertoires differ. M. cerasi assembled in 406 Mbp in 49,349 contigs with an N50 of 23,265. CEGMA and BUSCO analysis suggests the relative completeness of the assembly is comparable with other aphid genome assemblies. DNA was extracted using Qiagen Blood Tissue extraction kit, library preparation was performed and sequenced at TGAC, Norwich UK. Library preparation was PCR-free aiming for insert size of ~500bp.
Myzus cerasi is a relative of Myzus persicae, they differ hugely in their host range. We sequenced the genome of M. cerasi and compared this to other available aphid genomes, including M. persicae. We found that relatively little synteny has been conserved between M. cerasi and M. persicae (when compared to other aphids) and their effector repertoires differ. M. cerasi assembled in 406 Mbp in 49,349 contigs with an N50 of 23,265. CEGMA and BUSCO analysis suggests the relative completeness of the assembly is comparable with other aphid genome assemblies. DNA was extracted using Qiagen Blood Tissue extraction kit, library preparation was performed and sequenced at TGAC, Norwich UK. Library preparation was PCR-free aiming for insert size of ~500bp.