PRJNA286149
Leptospirillum group II CRISPR loci Raw sequence reads
Bacterial CRISPR-Cas systems provide insight into recent population history because they rapidly incorporate, in a unidirectional manner, short fragments (spacers) from coexisting infective virus populations into host chromosomes. Here, we used metagenomics to study the stability and dynamics of the type I-E CRISPR-Cas locus of Leptospirillum group II bacteria in biofilms sampled from an acid mine drainage (AMD) system. The entire Leptospirillum group II CRISPR locus was amplified with specific CRISPR primers and sequenced via 454.