GSE161695
RNA-binding profiles of CKAP4  in human myocardial tissues
CKAP4 as one of cardiac activated-myofibroblast protein markers may be involved in cardiac fibrosis. We assumed that CKAP4 hold a role in the regulation of cardiac fibrotic remodeling as an RNA-binding protein. Using improved RNA immunoprecipitation and sequencing (iRIP-seq), we sought to analyze the RNAs bound by CKAP4 in normal atrial muscle (IP1 group) and remodeling fibrotic atrial muscle (IP2 group) from patients with cardiac valvular disease.Binding peak-related gene cluster enrichment analysis showed these genes were mainly involved in biological processes such as signal transduction, protein phosphorylation, axonal guidance, and cell connection. Through gene annotation and correlation analysis of binding-peaks reads, we found that at least four lncRNAs including LINC00504, FLJ22447, RP11-326N17.2 and HELLPAR were CKAP4 potential binding lncRNAs in myocardial tissues.We reveal certain RNA-binding features of CKAP4 suggesting a relevant role as an unconventional RNA-binding protein in cardiac remodeling process. Overall design: iRIP-seqencing in normal atrial muscle (IP1 group) and remodeling fibrotic atrial muscle (IP2 group) from patients with cardiac valvular disease.