GSE122638
Widespread chromatin activation underlies pathogenic mechanisms in multiple myeloma
RNA-seq analysis comparing the transcriptional landscape of PRDM5 silenced vs mock KMS-11 multiple myeloma cell line Overall design: KMS-11 multiple myeloma cell line was transduced with an inducible shRNA expressing lentiviral system (Tet-pLKO-puro, Addgene #21915) targeting PRDM5 gene. shRNA expression was induced by adding 1 µg/ml doxycycline (Sigma) to the culturing media and replaced every 48 hours. Samples were harvested after 4 days of shRNA expression induction with doxycycline.