GSE173367
RNA Sequencing of npr-15(tm12539) and  wild type (WT) N2 C elegans
Purpose: To uncover immune genes and pathways that are modulated by the GPCR/NPR-15 Methods: RNA was extracted from synchronized L4 stage npr-15(tm12539) and WT animals grown at 20 C using Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, synaptic signaling and metabolism genes and pathways that are dependent on NPR-15 Conclusions: Our study uncovered NPR-15 to be modulator of the innate immunity in C. elegans Overall design: RNA seq  profiles of L4-stage npr-15(tm12539) and wild type (WT) C elegans animals were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000 platform.