GSE171221
Non-deletional CD8+ T cell self-tolerance permits responsiveness but limits tissue damage
Self-specific CD8+ T cells often escape clonal deletion, but the properties and capabilities of such cells in a physiological setting are unclear. We characterized polyclonal CD8+ T cells specific for the melanocyte antigen tyrosinase-related protein 2 (Trp2) in mice that express or lack this enzyme due to deficiency in Dct, which encodes Trp2. The size, phenotype, and gene expression profile of the pre-immune Trp2/Kb-specific pool were similar in wild-type (WT) and Dct-deficient (Dct-/-) mice. Despite comparable initial responses to Trp2 immunization, WT Trp2/Kb-specific cells showed blunted expansion, and scRNAseq revealed WT cells less readily differentiated into a CD25+ proliferative population. Functional self-tolerance clearly emerged when assessing immunopathology: adoptively transferred WT Trp2/Kb-specific cells mediated vitiligo much less efficiently. Hence, CD8+ T cell self-specificity is poorly predicted by precursor frequency, phenotype or even initial responsiveness, while deficient activation-induced CD25 expression and other gene expression characteristics may help to identify functionally tolerant cells. Overall design: Comparison of tolerant (WT) versus non-tolerant (KO or Dct-/-) CD8+ Trp2-specific cells. Number, phenotype, expansion, and functionality were compared. This analysis included gene expression comparisons between Trp2-specific cells from WT vs. KO/Dct-/- naive mice (bulk RNAseq in pre-immune mice) and mice treated with TriVax 3 or 7 days prior (single cell [sc] RNAseq).