GSE65865
Low-grade glioma microglia
To examine the effect of Nf1 inactivation in astrocytes on normal microglia in the optic nerve, microglia from Nf1 flox/flox (FF) and Nf1 flox/flox; GFAP-Cre (FFC) were flow sorted. High throughput RNA-seq was employed to compare the expression profiles, and upregulated genes in microglia supportive to Nf1 deficient astrocytes were verified. Overall design: Microglia from mice lacking Nf1 in astrocytes were flow sorted. Upregulated genes were verified and further examined.