ena-STUDY-UNIVERSITY OF GLASGOW-25-10-2018-12:01:54:304-319
Deep genomic sequencing results from a screen of EMS mutants of LOV2Kinase, a truncated version of phototropin 1
The LOV2Kinase transgenic line was mutated with EMS to uncover suppressors of its activity in low light conditions. Following the identification of suppressor mutants, each mutant was backcrossed against the LOV2Kinase parent and subjected to deep sequencing to identify candidate SNPs that could act as suppressors.
The LOV2Kinase transgenic line was mutated with EMS to uncover suppressors of its activity in low light conditions. Following the identification of suppressor mutants, each mutant was backcrossed against the LOV2Kinase parent and subjected to deep sequencing to identify candidate SNPs that could act as suppressors.