RNG EZ Exome 2
Targeted human exome enrichment using SeqCap EZ Human Exome Library v2.0 from Roche NimbleGen
The human HapMap sample NA19240 (YRI) was captured three times, twice using the v2.0 protocol (gel free) and once using the v1.2 protocol (gel cut) for comparison of the results from the 2 protocols. The detailed v1.2 and v2.0 protocols are available at www.nimblegen.com. The NA19240 sample was chosen for comparison against publically available SNPs found in the same sample by Complete Genomics and the 1000 Genomes Project. Another human HapMap sample, NA12762 (CEU), was captured 4 separate times with the v2.0 protocol, each time with a different production lot of the Roche NimbleGen EZ Exome v2.0 library.
Targeted sequencing of the exome has emerged as an efficient method for detection of SNPs and other genomic variations with functional impact. Roche NimbleGen has developed the SeqCap EZ Exome Library v2.0 and gel free protocol for comprehensive and easily automatable investigation of the human exome. The EZ Exome v2.0 design is based on NCBI Reference Sequence (RefSeq) RefGene from UCSC (January 2010), CCDS from NCBI (September 2009), miRBase v.14 (September 2009), and customer inputs. Here we present 7 lanes of Illumina paired-end sequence sets from captures of 2 human HapMap samples: NA19240 (YRI) and NA12762 (CEU).