DRP004525
PCR dropout experiments in Kuroshio Tank, Churaumi Aquarium
To evaluate changes in detection rate of fish species with (1) number of filters; (2) number of PCR replicates; and (3) annealing temperatures of 1st-round PCR, we used tank waters of the Kuroshio tank in Churaumi Aquarium and performed environmental DNA metabarcoding analysis using MiFish universal primers following Miya et al. (2015).