PRJNA214532
High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity
To better understand the effects of spo11 mutations on meiotic gene conversion and crossing over, we monitored meiotic recombination on a genome-wide scale by sequencing the genomes of spores from tetrads containing four viable spores. For this analysis, we used a diploid derived by mating a typical laboratory strain (S96) to a clinical isolate (YJM789). The parental strains differ from each other at ~55,000 locations (single-nucleotide polymorphisms (SNPs) allowing most interhomolog recombination events to be detected.  Spores from five tetrads derived from spo11 hypomorphs (three from spo11-217 and two from spo11-32, sporulated at 24 or 31.5 oC) were subjected to high-throughput, multiplexed sequencing.