GSE120922
R-loop landscapes in Rice (Oryza sativa) [DRIP-seq]
The R-loop is a common chromatin feature presented from prokaryotic to eukaryotic genomes and has been revealed to be involved in multiple cellular processes and associated with many human diseases. Here, we take the advantage of our recently developed ssDRIP-seq method to profile genome-wide R-loop levels and provided a first-hand R-loop atlas of Rice (Oryza sativa) at different developmental stages. Overall design: ssDRIP-seq in Oryza sativa. The gDNA was extracted from different tissues, and fragmented by restriction enzymes. By using S9.6 antibody, DNA-RNA hybrids were pulled down. Based on ssDNA library kit, the ssDRIP library was prepared and sequenced.