Transcriptome profiling of black pepper (Piper nigrum L.)
De novo transcriptome assembly of black pepper (Piper nigrum L.)
The tender leaves collected from potted black pepper plant (variety –Panniyur 1) were used for extraction of total RNA. A standard cDNA library was constructed and sequenced via Illumina HiSeq2000 in Beijing Genomics Institute (Shenzhen, China).
Genome sequence information of black pepper unlike model species in public databases is very less for black pepper. Hence transcriptome profiling based on illumina paired-end sequencing has permitted a significant enhancement in discovery and expression profiling of genes and functional genomics studies in black pepper.