ena-STUDY-UNIVERSITY OF WARMIA AND MAZURY-23-01-2019-12:09:14:127-836
Hybrid sequencing of Hymenolepis diminuta genome
The aim of this study was to sequence and assemble de novo Hymenolepis diminuta (HD) genome using combination of three methods. DNA was isolated from laboratory strain WMS-il1 underwent Next Generation Sequencing using the Illumina HiSeq 1500 platform. Additional sequencing was performed using a MinION sequencer from Oxford Nanopore Technologies (ONT) and mentioned earlier Illumina HiSeq 1500 system. For Illumina sequencer two types of data were obtained: paired-end tags (mean insert size c.a. 400 bp) and mate-pairs (mean insert size c.a. 7 kbp). In this study we showed, that merging data from another sequencing platforms could greatly improve the final results of de novo assembling. Especially, combining short DNA reads obtained from next-generation sequencing with long DNA reads obtained from third generation sequencing could significantly improve the length and the quality of the resultant DNA sequences. Moreover, the increasing of coverage of the each sequencing technology above the certain level did not increase assembly results significantly, what we also showed in this study. Our study show that combination of emerging genomic technologies is useful approach in producing accurate de novo assemblies of helminth genomes.
The aim of this study was to sequence and assemble de novo Hymenolepis diminuta (HD) genome using combination of three methods. DNA was isolated from laboratory strain WMS-il1 underwent Next Generation Sequencing using the Illumina HiSeq 1500 platform. Additional sequencing was performed using a MinION sequencer from Oxford Nanopore Technologies (ONT) and mentioned earlier Illumina HiSeq 1500 system. For Illumina sequencer two types of data were obtained: paired-end tags (mean insert size c.a. 400 bp) and mate-pairs (mean insert size c.a. 7 kbp). In this study we showed, that merging data from another sequencing platforms could greatly improve the final results of de novo assembling. Especially, combining short DNA reads obtained from next-generation sequencing with long DNA reads obtained from third generation sequencing could significantly improve the length and the quality of the resultant DNA sequences. Moreover, the increasing of coverage of the each sequencing technology above the certain level did not increase assembly results significantly, what we also showed in this study. Our study show that combination of emerging genomic technologies is useful approach in producing accurate de novo assemblies of helminth genomes.