GSE134688
Bulk RNA-seq of mouse cells from the dorsal cerebral cortex during its formation
Dorsal cortices from embryonic day (E10.5) to postnatal day 1 (PN) were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs. Overall design: 70 samples of neural stem cells, basal progenitors and newborn neurons, daily from embryonic day 10.5 to first post-natal day.