Developmental stages of P. parvimesis
Transcriptome analysis  of 2 developmental stages (2 day old gastrula and 6 day old larvae) of sea cucumber (P. Parvimesis)
Gravid P. parvimensis adults were spawned by heat shock and embryos grown in artificial sea water at 15 degrees Celsius. Total RNA was extracted from 2 day old gastrula and 6 day old larvae using the Total Mammalian RNA Miniprep kit (Sigma). RNA was sent to the Wistar Institute for library preparation with Illumina adaptors and 72bp paired-end sequencing was performed on a Solexa Genome Analyzer II. First strand cDNA synthesis was performed with the iScript Select cDNA Synthesis Kit.
We generated RNA-Seq data to study the development of the sea cucumber Parastichopus parvimensis. Our corrected assembled transcripts shed new light on two developmental stages and revealed novel transcripts.