GSE138821
The role of DOT1L in Th cells
ChIP sequencing of naïve Th, bona fide Th1 and bona fide Th2 cells Overall design: Cells were sorted from the spleens of WT mice for naïve Th cells. Bona fide Th1 or Th2 were generated by culturing enriched Th cells for 4 days under Th1 or Th2 polarising conditions. IL-4 Amcyan - IL-13 dsRed - IFNg-YFP reporter mice were used to sort bona fide Th1 (YFP+) or bona fide Th2 (Amcyan or dsRed+) cells.