GSE200483
RNA-seq on ES cells where DDX6 was knocked down by the AID method
To degrade DDX6 acutely, we adopted an advanced protein degradation method, the auxin-induced degron (AID) system. For this purpose, we prepared an AID-tagged DDX6 and E3 ligase subunit of OsTIR1 in an engineered ES (D6AdP cell line). These deposited data are RNA-seq data on these D6AdP ES cells treated with the auxin analog (IAA) treatment for 0, 3, and 6 hours. Overall design: Single-end RNA-seq of polyA RNAs originally from 500-1000 ng of total RNAs extracted from the D6AdP ES cells treated with the auxin analog (IAA) treatment for 0, 3, and 6 hours, processed with the Truseq Stranded mRNA Library Prep kit (Illumina).