GSE116636
Novel Race-specific Genetic and Epigenetic Determinants of ADT-Resistance in African American Prostate Cancer
Purpose: The goals of this study is to compare NGS-derived Androgen Deprivation Therapy (ADT) resistant  miRNome  profiling to  Androgen Deprivation Therapy (ADT) sensitive miRNome profile in African American prostate cancer cells  and validate  by reverse transcription polymerase chain reaction (qRT–PCR) methods. Method: Mirco-RNA were isolated from different CaP cells who were sensitive and resistant to Androgen Deprivation Therapy  (ADT).  Total micro-RNA were subjected to miRNA-Seq. Results: We performed whole miRNA-Seq analysis through paired-end deep sequencing to systematically investigate the molecular features of different CaP  cell models- RC-77-N, RC-77T/E, RCC7T/E-ADT, RCC7T/E-CD133-plus, E006AA-hT and E006AA-hT-ADT. Conclusions:  Our study represents the first detailed analysis of African American ADT resistant miRNome, with biologic replicates, generated by miRNA-seq technology. Overall design: Androgen resistant and Androgen sensitive miRNA profiles from African American prostate cancer  of  were generated by deep sequencing, in duplicate using Illumina