GSE120846
Isolation and genome-wide characterization of cellular DNA:RNA triplex structures II
RNA can directly bind to purine-rich DNA via Hoogsteen base pairing, forming a DNA:RNA triple helical structure that anchors the RNA to specific sequences and allows guiding of transcription regulators to distinct genomic loci. To unravel the prevalence of DNA:RNA triplexes in living cells, we have established a fast and cost-effective method that allows genome-wide mapping of DNA:RNA triplex interactions. In contrast to previous approaches applied for the identification of chromatin-associated RNAs, this method uses protein-free nucleic acids isolated from chromatin. High-throughput sequencing and computational analysis of DNA-associated RNA revealed a large set of RNAs which originate from non-coding and coding loci, including repeat elements. Combined analysis of DNA-associated RNA and RNA-associated DNA identified genomic DNA:RNA triplex structures. The results suggest that triplex formation is a general mechanism of RNA-mediated target-site recognition, which has major impact on biological functions. Overall design: We used biotinylated RNA oligonucleotides corresponding to two triplex-forming regions in NEAT1 (TFR1 and TFR2) to capture their putative target loci. TFR-associated DNA from biological triplicates was sequenced (TFR1_rep1-3 and TFR2_rep1-3). Experiments with an oligonucleotide that does not contain a potential TFR served as control (Control_rep1-3).