GSE131073
RNAseq analysis of lacrimal glands isolated from wild type and Aire-/- mice at 5 weeks of age
We used freshly isolated lacrimal glands isolated from wild type and Aire-/- mice on a Balbc background. Mice were 5 weeks of age, with an n=4 for wild type and n=3 for Aire-deficient. Overall design: Lacrimal gland mRNA profiles of 5 week old wild type (WT) and Aire-/- mice were generated by deep sequencing, in triplicate (Aire-/-) or quadruplicate (wild type), using Illumina HiSeq4000.