GSE114589
CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.
We obtained  RNA polymerase II occupancy profiles across the genome of S.cerevisiae  strains:   Abf1 anchor-away cells after addition of  rapamycin for different time points (including no addition of rapamycin) and  Rap1-AID auxin-degron cells after addition of auxin for different time points (including no addition of auxin) .  This allowed us to compare polymerase occupancy profiles when these proteins are depleted from the nucleus or degraded. Overall design: The nascent RNA was crosslinked to RNAPII and sequenced after purification of Rpb1 under stringent and denaturing conditions. This reveals the position of the polymerase at the nucleotide resolution level.