GSE135476
Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast
For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells. Overall design: We used centrifugal elutriation to obtain our synchronous cell cultures. From ~100 different elutriated cultures, we generated a cell size-series, spanning a range from 40 to 75 fL, sampled approximately every 5 fL intervals, in three biological replicates in each case. The same 24 distinct pools were aliquoted as needed to generate the input samples for measurements of RNA (with RNAseq), proteins (with LC MS/MS), and metabolites (GC-TOF MS for primary metabolites; HILIC-QTOF MS/MS for biogenic amines; and CSH-QTOF MS/MS for lipids). Here, we deposit the RNA datasets.