GSE132600
Camelina sativa, salinity tolerance, 1-aminocyclopropane-1-carboxylate deaminase, plant growth promoting bacteria, transgenic plants
Growth in soil inoculated with plant growth promoting bacteria (PGPB) producing 1-aminocyclopropane-1-carboxylate |(ACC) deaminase or expressing of the corresponding acdS in transgenic lines reduces the decline in shoot length, shoot weight and photosynthetic capacity triggered by salt stress in Camelina sativa.  Reducing the levels of stress ethylene decreases the expression of salt stress-responsive genes, specifically genes involved in development, senescence, chlorosis and leaf abscission that are highly induced by salt to the levels that may have a less negative effect on growth and productivity. Moderate expression of acdS under the promoter of the rolD promoter or growing plants in soil treated with the PGPB Pseudomonas migulae 8R6, were more effective in eliminating the expression of the genes involved in ethylene production and/or signaling than expression under the more active Cauliflower Mosaic Virus 35S promoter. Overall design: Four experimental conditions were tested:  the parental Camelina (DH55), a transgenic with acdS contitutively expressed (35S::acdS), a transgenic with acdS most highly expressed in roots  (rolD::acdS), and DH55 exposed to a plant growth promoting bacteria expressing acdS (8R6).  Camelina shoots (DH55, 35S::acdS, rolD::acdS, PGPB (8R6)) with salt treatment were analyzed.  3 replicates of each were compared.