PRJNA448275
RNA-seq to study circular RNAs of wild type C57 mouse whole sperm and sperm droplet
Given that circRNAs are much more stable than linear RNAs, we hypothesized that a small amount of circRNAs might be present in sperm. We purified 1) cytoplasmic droplets from spermatozoa collected from the whole epididymis and 2) whole sperm cells to study the sperm-borne circRNAs.