DRP005495
Ketone body receptor GPR43 regulates lipid metabolism under fasting condition
C57BL6/J and GPR43 deficient mice were randomly assigned to exam lipid metabolism by ketone bodies, each mouse was fasted for 48 h. Cecals were collected at the end of treatments. Cecal DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions v4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 10pM: 'Preparing Libraries for Sequencing on the MiSeq'.