PRJNA390869
Prunus dulcis cultivar:Nonpareil and Lauranne Raw sequence reads
Almond is a tree crop and an outcrossing species with a gametophytic self-incompatibility system. Almond linkage maps have been constructed widely using isozyme, restriction fragment length polymorphisms (RFLP), SSRs, RAPDA and SCAR and ISSR markers. Despite the fact that single nucleotide polymorphisms are the most abundant differences in plants, very limited usage in almond breeding can be seen. Next-generation sequencing (NGS) enables discover and directly assay large numbers of sequence polymorphisms without prior knowledge about the polymorphisms or their genomic positions. Among various available reduced representation library preparation protocols, the genotyping-by-sequencing (GBS) is simple and makes it possible to discover thousands of SNPs.