GSE25800
GSE25800: Identification of Transcription Factor MEP-1::GFP Binding Regions in Embryos
Summary:     modENCODE_submission_2600  This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648  Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals.  Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.  For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf  Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP70(made_by : R Waterston tags : GFP::3xFlag mutagen : Bombard genotype : unc119(ed3);wgIs70(mep-1::TY1 EGFP FLAG C;unc119) official name : WBGene00003218 ); Developmental Stage: embryo; Genotype: unc119(ed3);wgIs70(mep-1::TY1 EGFP FLAG C;unc119); Sex: Hermaphrodite;  EXPERIMENTAL FACTORS: Developmental Stage embryo; Target gene mep-1; Strain OP70(made_by : R Waterston tags : GFP::3xFlag mutagen : Bombard genotype : unc119(ed3);wgIs70(mep-1::TY1 EGFP FLAG C;unc119) official name : WBGene00003218 ); temp (temperature) 20 degree celsiusSeries_type: CHIP-seq