GSE161323
Factor Xa and factor IIa inhibitors differentially regulate inflammation in myocardial ischemia reperfusion injury [19-0267 RNA-seq]
While thrombin is the key protease in regard to thrombus formation, other coagulation proteases, such as fXa or activated protein C (aPC), independently modulate intracellular signaling via partially disjunct receptors. Hence, we postulate that inhibition of fXa or fIIa conveys different effects in myocardial ischemia-reperfusion injury (IRI) in regard to inflammation despite comparable anticoagulant efficacy. Overall design: Wild-type mice (C57BL/6, age 8-10 weeks) received fXai (3 mg/kg) or fIIai (10 mg/kg) for 7 days prior to myocardial IRI surgery. Myocardial ischemia reperfusion was induced by LAD ligation for 90 min followed by 24 h of reperfusion. Drugs treatment was reinitiated 2 h post-surgery and continued for 24 h. Mice were sacrificed at the end of study period and heart samples including infarcted part were isolated and RNAseq was performed.