ena-STUDY-Section of biotechnology-14-08-2014-08:38:27:814-339
Global changes in Staphylococcus aureus gene expression during human prosthetic joint infection
Little is known about regulation of gene activity of the major pathogen Staphylococcus aureus during actual human infection. Here we characterize the metabolome using NMR, and the transcriptome using deep RNA sequencing, of S. aureus infected joint fluid derived from an acute human prosthetic joint infection, and compare it with the genome, transcriptome and metabolome of an isolate obtained from the same sample grown in vitro (LB medium).  The transcriptome indicated that the bacterial infection sustains on a versatile human-cell-based diet consisting of amino acids, glycans and nucleosides, since significant upregulation of genes involved in the catabolic degradation pathways of these compounds were observed in situ. This is consistent with metabolite analysis of the infected joint fluid and of S. aureus culture supernatants where the concentration of most amino acids, some amino sugars were found to be higher in the joint fluid than in vitro culture supernatants, whereas the concentration of glucose was higher in culture supernatant. Furthermore, presumably because of oxygen limitations in the joint fluid, transcriptomic evidence for fermentation was observed, consistent with the presence of fermentation products (ethanol) in situ. Moreover, many, but not all, of the known virulence factor genes were upregulated in situ.
Little is known about regulation of gene activity of the major pathogen Staphylococcus aureus during actual human infection. Here we characterize the metabolome using NMR, and the transcriptome using deep RNA sequencing, of S. aureus infected joint fluid derived from an acute human prosthetic joint infection, and compare it with the genome, transcriptome and metabolome of an isolate obtained from the same sample grown in vitro (LB medium).  The transcriptome indicated that the bacterial infection sustains on a versatile human-cell-based diet consisting of amino acids, glycans and nucleosides, since significant upregulation of genes involved in the catabolic degradation pathways of these compounds were observed in situ. This is consistent with metabolite analysis of the infected joint fluid and of S. aureus culture supernatants where the concentration of most amino acids, some amino sugars were found to be higher in the joint fluid than in vitro culture supernatants, whereas the concentration of glucose was higher in culture supernatant. Furthermore, presumably because of oxygen limitations in the joint fluid, transcriptomic evidence for fermentation was observed, consistent with the presence of fermentation products (ethanol) in situ. Moreover, many, but not all, of the known virulence factor genes were upregulated in situ.