GSE168396
Joint analysis of transcriptome and metabolome reveals the anti-low temperature mechanism of Hosta ventricosa
In this study, a total of 12,059 differential genes and 131 differential metabolites were obtained, and they were mainly concentrated in signal transduction and phenylpropane metabolic pathways. In the process of low temperature signal transduction, the hosta is mainly through the ion channels on the three cell membranes of COLD, CNGCs and CRLK to transmit Ca2+ inside and outside the cell to sense temperature changes, and stimulate SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL. Strengthen the low temperature resistance of the purple calyx hosta. The phenylpropane metabolic pathway and the flavonoid metabolism pathway are the main metabolic pathways for the hosta to resist low temperature stress. The hosta protects itself from low temperature damage by increasing the content of Genistein, Scopolentin and Scopolin. It is speculated that the hosta also adjusts Sinapyl alcohole and Coniferyl alcohol content ratio can change the morphological structure of cell wall and enhance low temperature resistance. Overall design: Transcriptome comparison between samples treated at 3°C for 7 days and samples treated at 25°C at room temperature