ena-STUDY-IBE(CSIC-UNIVERSITAT POMPEU FABRA)-09-08-2018-11:59:51:678-448
Single molecule sequencing and assembly of flow sorted Y chromosome from HG02982
Mammalian Y chromosomes are often neglected from genomic analysis. Due to their inherent assembly difficulties,  high repeat content, and large ampliconic regions, only a handful of species have their Y chromosome properly characterized. To facilitate the assembly of the Y, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device to create a highly continuous representation of the first human Y chromosome of African origin.
Mammalian Y chromosomes are often neglected from genomic analysis. Due to their inherent assembly difficulties,  high repeat content, and large ampliconic regions, only a handful of species have their Y chromosome properly characterized. To facilitate the assembly of the Y, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device to create a highly continuous representation of the first human Y chromosome of African origin.