E-MTAB-10756
RNAseq of Tregs before and after treatment with Multipep
Intent: to investigate whether treatment of an individual with peptide immunotherapy using MultiPepT1De results in a change in the transcriptional profile of CD4+ regulatory T cells.  Regulatory T cells (CD4+ CD25+ CD127lo) were isolated by flow cytometry cell sorting from cryopreserved PBMC from subjects before and after treatment with MultiPepT1De. RNA was extracted using the AllPrep micro kit (Qiagen) and RNAseq libraries prepared from extracted RNA (91-140 ng)
Intent: to investigate whether treatment of an individual with peptide immunotherapy using MultiPepT1De results in a change in the transcriptional profile of CD4+ regulatory T cells.  Regulatory T cells (CD4+ CD25+ CD127lo) were isolated by flow cytometry cell sorting from cryopreserved PBMC from subjects before and after treatment with MultiPepT1De. RNA was extracted using the AllPrep micro kit (Qiagen) and RNAseq libraries prepared from extracted RNA (91-140 ng)