GSE210637
ER, mitochondria and ISR regulation by mt-HSP70 and ATF5 upon procollagen misfolding (proximal tibia srRNASeq)
We used spatially resolved RNA sequencing (srRNA-seq) to identify trabecular osteoblast cell stress response pathway to misfolding of type I procollagen with Gly610->Cys substitution in the triple helical region of alpha2(I) chain (trabecular region of proximal tibia adjacent to the growth plate). Overall design: Cryosections of fresh frozen proximal tibia were examined by srRNASeq as described in Gorrell et al, Adv Sci, 2022