GSE209918
Alterations in gonadotropin, apoptotic and metabolic pathways in granulosa cells warrant superior fertility of the Dummerstorf high fertility mouse line 1 (FL1)
During the last decades, several mouse models provided insights into the regulation of folliculogenesis. In contrast to the commonly used transgenic or knockout mouse models, the Dummerstorf high-fertility mouse line 1 is a worldwide unique selection experiment for increased female reproductive performance and high fertility. These mice are characterized by various alterations on endocrine and molecular levels, which have the potential to improve folliculogenesis and therefore lead to increased ovulation rates. To get insights into the cellular mechanisms leading to the high fertility phenotype we used granulosa cells isolated from antral follicles for mRNA sequencing and measured hormones and growth factors associated with follicular development.  Our results indicate that the crosstalk between different intracellular signaling pathways in the granulosa cells are improved, follicular atresia in FL1 might be decreased due to enhanced granulosa cell survival and by improving the efficiency of intracellular signaling, glucose metabolism and signal transduction, FL1 mice have several advantages in reproductive performance and therefore increased the ovulation rate without health issues. Overall design: Comparative gene expression profiling analysis of RNA-seq data for granulosa cells isolated from antral follicles derived from Dummerstorf ctrl and FL1 mice