GSE112920
IL-27 in vaccine elicited cellular immunity
5000 congenically marked (CD45.1) WT or IL-27R-/- OT1 T cells were transferred into WT B6 hosts (CD45.2). One day later, the mice were immunized with ovalbumin (100 µg), PolyIC (40 µg) and anti-CD40 antibody (FGK4.5, 40 µg).  3 days after immunizayion, spleens were harvested and the OT1 T cells isolated by flow sorting based on CD45.1 expression. Each sample is the result of pooling T cells from 3 individual mice.  RNA was isolated from the sorted cells and RNAseq performed. Overall design: RNAseq of transferred WT and IL-27R-/- OT1 T cells isolated 3 days  after combined polyIC/aCD40 immunization