GSE184806
Investigation of human peripheral T cells responsive to SARS-CoV-2 antigens
To elucidate the T cell epitopes of SARS-CoV-2, we stimulated human PBMCs from healthy donors and convalescent COVID-19 patients with various SARS-CoV-2 antigens, sorted the activated T cells and performed sc-RNA and -TCR sequencing. We obtained thousands of T cell clonotypes that responded to SARS-CoV-2 antigens, and identified the epitopes and restricting HLAs of several clonotypes that were significantly expanded in the COVID-19 patients. Overall design: Determination of the TCR and mRNA expression of human T cells stimulated with SARS-CoV-2 antigens.