GSE171845
Transcriptional adaptation to DNA replication stress induced by CCNE1 overexpression
We overproduced human cyclin E from a stably-integrated  inducible CCNE1 transgene in RPE1-hTERT cells over a period of weeks. CCNE1 overproduction induced replication stress, then slow proliferation, followed by adaptation to normal growth phenotype. We analyzed mRNA transcripts at key points of the timecourse: control, 2 days, 15-18 days, 39-51 days, and at a point several weeks after induction was withdrawn Overall design: Retinal pigmented epithelial (RPE1-hTERT) mRNA profiles of CCNE1 overexpression in adaptation experiment over period of up to 51 days