GSE131998
RNAseq of Extracellular vesicle RNAs and cellular RNAs from HPASMCs with or without TGF-b1 or BMP4 treatment
Excessive TGF-ß signalling has been shown to underlie pulmonary hypertension (PAH). Human pulmonary artery smooth muscle cells (HPASMCs) can release extracellular vesicles (EVs) but their content and significance have not yet been studied. Here, we analysed the content and biological relevance of HPASMC-derived EVs. We used low-input RNA-Seq to analyse the RNAs of EVs released by HPASMC under basal conditions. This data was compared to cellular RNAs. The same experiments (RNA-seq of EV and cellular RNAs) were carried out on HPASMC treated with TGF-b1 and BMP4, recapitulating pathological conditions. Overall design: Extracellular vesicles were isolated from HPASMC with or without TGF-b1 or BMP4 treatment. Low-input RNA-seq was performed on EV and cellular RNAs by BGI. Three replicates were carried out and sequenced for most conditions except for EV from untreated and BMP4 treated HPASMC with 2 replicates due to failure of the library preparation of one replicate.