GSE105792
T47D RNA-seq and ChrRNA-seq data
We performed genome-wide RNA-seq and ChrRNA-seq experiments in T47D cell lines  after PADI2 knock down . Both the mRNA and  chromatin RNA seq  peformed in sicontrol  and siPADI2  as in biological replicates . Also we performed  mRNA seq in T47D cells expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2. Overall design: We designed experiment to perform   mRNA  sequencing in T47D after PADI2 knock down as in biological replicate experiemnt .  Given the fact we found significant number of genes differentailly expressed after PADI2 knockdown in T47D cells, we designed to perform Chromatin RNA seq in  T47D cells after PADI2 knock down as in biological replicates.  We find that PADI2 deiminates R1810 at CTD (C-terminal domian)  of RNAP2 , So in order to analyse the mRNA  gene expression level in T47D cells expressing amanitin resistant  HA - tagged wild type and mutant form of RNAP2 , we performed mRNA seq as in biological replicate in T47D cells expressing   amanitin resistant  HA - tagged wild type and mutant form of RNAP2.