PRJNA679793
Linkage mapping of Melanotic gene in chicken
To apply linkage mapping of Melanotic gene, a three-generation population was constructed, initiated by crossing two Partridge Plymouth Rock males with four Dark Cornish females. Matings between 17 F1 females and 2 F0 Partridge Plymouth Rock males produced 126 female backcross individuals. Based on the plumage patterns, chickens with penciling (n = 60), double lacing (n = 43), or intermediate patterns (n = 23) in the backcross population were classified. Two DNA Pools were constructed using the parental individuals in our mapping population (Pool_Cornish and Pool_Plymouth_Rock, pooling sizes are 4 and 2, respectively). They were subjected to whole genome sequencing together with two pools of backcross individuals (Pool_pencilling and Pool_double_lacing, pooling sizes are 60 and 43, respectively). SRA data for Pool_Plymouth_Rock was submitted to PRJNA679787 as SAMN16846358.