GSE167374
BRD4-chromatin interaction in reporter KBM7 cells (REDS15) with purine metabolism perturbation
To investigate whether BRD4-chromatin binding is modulated by purine levels, we performed BRD4 ChIP-seq in cells with SLC nucleoside transporters knockout, or cells with de novo purine synthesis inhibitor 6-MP treatment. (+)-JQ1, an inhibitor which disrupts BRD4 and acetylated histone binding, was used as a positive control. Overall design: BRD4-ChIP seq in REDS15 with sgRen, SLC35F5/SLC28A3/SLC29A4 triple knockout (SLC TKO) for 12 days, 10 micromilar 6-MP or 1 micromolar (+)-JQ1 treatment for 48 h