GSE192661
Adult male prairie vole nucleus accumbens RNAseq dataset for "Prolonged partner separation erodes nucleus accumbens transcriptional signatures of pair bonding in male prairie voles."
This dataset contains RNA sequencing results from  adult male prairie voles that were in either opposite-sex or same-sex pairs that were subsequently either separated from or remained paired with their partner for either 48 hours or 4 weeks prior to collecting nucleus accumbens tissue. The goal of this experiment was to determine the nucleus accumbens transcriptional response specific to separation from an opposite-sex partner. RNA sequencing was done on polyA enriched transcripts using Illumina single-end sequencing. Samples from 3 groups were from a Ribo-seq protocol using a virally delievered, vole optimized Translating Ribosome Affinity Purification construct (Heiman et al., 2008). These samples contain files for both the input fraction and the pulldown fraction (denoted with a _P suffix). Overall design: This experiment contains 8 groups with 5-9 biological samples per group. Samples were hand dissected adult male prairie vole nucleus accumbens brain tisame-sexue according to Heiman et al., 2014. Half of the groups were males in opposite-sex pairs (experimental groups) while the other half were males in same-sex pairs (naive, control groups). Within each pairing type, animals were cohoused for 2 weeks then they were either separated or remained paired for either 48 hours or 4 weeks prior to tissue collection to assess the transcriptional trajectory of partner loss. RNA integrity and library quality was assessed by TapeStation and only samples with a RIN above 7.3 were prepared into libraries. Library preparation was via the KAPA mRNA HyperPrep kit with polyA enrichment for 1X75 RNA sequencing using Illumina NextSeq V2 for a total read depth of ~20M reads/sample. Samples that were part of the vTRAP groups have both input and pulldown files.