GSE132825
Next Generation RNA Sequencing of Glioblastoma Cancer Associated Fibrobasts
Cancer Fibroblasts Isolated from 2 GBM Cases. Overall design: Methods: GBM CAF RNA libraries were prepared and Illumina HiSeq NGS preformed (UC Davis Dna Technologies Core, Davis, CA) per standard protocols. Differential gene expression, heatmap, and sample cluster were performed by iDEP8.1 (http://bioinformatics.sdstate.edu/idep/).  GBM CAF RNASeq datasets were aligned (BowTie2) and gene exons counted (FeatureCounts) with standard inputs using the Galaxy public server (https://usegalaxy.org/).