GSE193931
HuR-dependent expression of Wisp1 is necessary for TGFbeta-induced cardiac myofibroblast activity
We show that activity of the RNA binding protein HuR is necessary for myofibroblast activation. This data set identifies directly bound HuR target genes in quiescent cardiac fibroblasts and active myofibroblasts. Overall design: RNA-sequencing of eluted HuR-bound targets following photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in primary isolated murine cardiac fibroblasts treated with vehicle control or 10 ng/ml TGFbeta