GSE125737
Niche-specific factors dynamically regulate sebaceous gland stem cells during skin homeostasis and aging
We deleted different Notch pathway components in different sub-compartments of mouse skin, and performed RNA-seq to assess differentially regulated genes, 10 weeks after deletion. Overall design: Mice were injected with tamoxifen at 8 weeks of age to delete either Rbpj (R) or Notch1 (N) in the skin.  Cre drivers were K14-CreERT (K) and Lrig1-CreERT2 (L).  Dorsal skin in telogen phase was harvested, isolated away from dermis, and extracted for total RNA by Qiagen RNeasy kit, 10 weeks post-tamoxifen.  LR = Lrig1-CreERT2+Rbpj-deletion;  KR = K14-CreERT+Rbpj-deletion;  N = K14-CreERT1+Notch1-deletion.   All 3 mutant strains had matched controls (Cont) from the same batch/litter.  See Veniaminova, Wong et al., 2019