GSE122801
m6A MeRIP-Seq of MEL624 control and PCIF1 KO cells
We analyzed m6A distribution by m6A MeRIP in otder to determine the effect of PCIF1 KO over m6A distribution in human melanoma cells Overall design: mRNA from two independent clonal PCIF1 KO cell lines and one control cell line was immunoprecipitated using an antibody against m6A. RNA-Seq libraries were generated from three biological replicates for Input (RNAseq) and m6A-immunoprecipitated mRNA. Libraries were sequenced in a HiSeq with an average coverage of 20 million reads per sample. Please note that the m6A rabbit monoclonal antibody from the EpiMark N6-Methyladenosine Enrichment Kit was used. This antibody is produced by Cell Signaling and distributed by NEB in the kit, but is not sold independently and doesn't have a catalog number.