DRP002625
Transcriptome Sequencing and Comparative Analysis of Two Types of Exosomes in Human Whole Saliva by Next Generation Sequencing
Exosomes are small membrane vesicles (30-100 nm in diameter) secrted by numerous cells. Exosome have been shown to contain mRNA and DNA. We found at least two types of salivary exosomes (exosome I and exosome II) that are different in size and have different proteome. In previous study, we performed small RNA transcriptome analysis by next generation sequencing technology and reported that many types of small RNA, such as miRNA, piwi-interacting RNA (piRNA), small nucleolar RNA (snoRNA), other small RNAs and genomic repeats were contained in exosome I, II and whole saliva. In this study, we performed whole-transcriptome analysis using cDNA libraries constructed from total RNA except small RNAs. We found that 11-32% of reads from salivary exosomes and whole saliva were mapped to pseudogene.  The Data Access Committee of the National Bioscience Database Center (NBDC) approved that this personal genetic data were made published according to NBDC data sharing guidelines (http://humandbs.biosciencedbc.jp/).