ena-STUDY-INRA-28-10-2016-14:03:41:638-344
Starvation effect on the chemosensory transcriptome of Spodoptera littoralis larvae
Fourth instar larvae of a lab strain of Spodoptera littoralis were used in this study. Antennae and maxillary palps were dissected from ~300 larvae (collected from 3 different generations) 24h after food deprivation and from ~300 larvae (collected from the same 3 different generations) that were not food-deprived. Total RNAs were extracted for each sample using TRIzol® Reagent (Invitrogen, Carlsbad, CA, USA) and used as templates for cDNA synthesis and Illumina sequencing by GATC Biotech SARL(HiSeq2000, one channel for the two samples, single read).
Fourth instar larvae of a lab strain of Spodoptera littoralis were used in this study. Antennae and maxillary palps were dissected from ~300 larvae (collected from 3 different generations) 24h after food deprivation and from ~300 larvae (collected from the same 3 different generations) that were not food-deprived. Total RNAs were extracted for each sample using TRIzol® Reagent (Invitrogen, Carlsbad, CA, USA) and used as templates for cDNA synthesis and Illumina sequencing by GATC Biotech SARL(HiSeq2000, one channel for the two samples, single read).