8446:4d013819-3805-4dc3-8e27-404ebd612215
Pelagic bacterial communities across Fram Strait
Water samples were collected in Fram Strait during the Polarstern cruise PS85 (June 6th 2014                 until July 3rd 2014) from eastern Greenland shelf to west coast of Spitsbergen. For assessing bacterial                 community composition 2 L of water were filtered with peristaltic pump through successive membrane                 filters of 3 µm and 0.22 µm. The extracted genomic bacterial DNA was isolated and the hypervariable                 V3-V4 (341F - 785R) region of the bacterial 16S rRNA was amplified and sequenced using Illumina MiSeq                 platform in a 2 × 300 bp paired-end run.