GSE210485
TAF2 and MYC cooperate to promote hepatocellular carcinoma
In order to check the tumorigenic property of TAF2, either alone or in combination with MYC, we used Sleeping Beauty (SB) transposase-mediated somatic integration in combination with hydrodynamic tail vein injection (HTVI) technique in FVB mice. TAF2 alone did not induce tumor, MYC overexpression induced tumors at a variable levels, while both TAF2 and MYC overexpression resulted in profound development of HCC. Overall design: FVB/NJ mice (6 weeks old weighing approximately 20 g) from The Jackson Laboratory were used for hydrodynamic injection of plasmids. For each mouse pT3-EF1?H-TAF2 (20 microg) or pT3-EF1alphaH-MYC (20 microg) vector was mixed with pCMV-SB vector (0.8 microg), diluted in 2 ml of saline, sterilized with a 0.22 micron filter and injected via tail vein within 5-8 seconds. In combination of pT3-EF1alphaH-TAF2 (20 microg) and pT3-EF1alphaH-MYC (20 microg), 1.6 microg pCMV-SB was used. Five weeks after injection mice were sacrificed and tumors were subjected to RNA-seq