E-MTAB-10469
RNAseq of Huh7 and Huh7.5 cells treated with interferon alpha, beta or lambda.
The transcriptome of Huh7 and Huh7.5 was sequenced from cells treated with interferon alpha (IFNa), beta (IFNß) or lambda (IFN?) for 8 hours. Each treatment was compared to respective mock-treated cells in 6 pairwise comparisons, for a total of 43 samples classified in 12 experimental conditions. Cells were treated for 8h with 10ng/ml of Interferon-a2A, 5000 U/ml of Interferon-ß1A or 100ng/ml of interferon-?3 prior RNA extraction and library preparation. Mock-treated cells were used as control for each treatment. 3 to 4 biological replicates were analysed for each biological condition. Libraries for Illumina sequencing were constructed by polyA selection with the Illumina Kit. A paired end (2x100) run was performed.
The transcriptome of Huh7 and Huh7.5 was sequenced from cells treated with interferon alpha (IFNa), beta (IFNß) or lambda (IFN?) for 8 hours. Each treatment was compared to respective mock-treated cells in 6 pairwise comparisons, for a total of 43 samples classified in 12 experimental conditions. Cells were treated for 8h with 10ng/ml of Interferon-a2A, 5000 U/ml of Interferon-ß1A or 100ng/ml of interferon-?3 prior RNA extraction and library preparation. Mock-treated cells were used as control for each treatment. 3 to 4 biological replicates were analysed for each biological condition. Libraries for Illumina sequencing were constructed by polyA selection with the Illumina Kit. A paired end (2x100) run was performed.