Genome-Wide Analysis of A-to-I RNA Editing via Single Molecule Sequencing in Drosophila
Genome-Wide Analysis of A-to-I RNA Editing via Single Molecule Sequencing in Drosophila
The accurate and thorough genome-wide detection of A-to-I editing, a biologically indispensable process, has proven challenging.    Here, we present a discovery pipeline in adult Drosophila with 3,581 high confidence editing sites identified with an estimated accuracy of 87 percent.    The target genes and specific sites highlight global biological properties and functions of RNA editing, including hitherto unknown editing in well-characterized classes of non-coding RNAs, and 645 sites that cause amino acid substitutions, usually at conserved positions.    The spectrum of functions subsumed by these gene targets suggests that editing participates in a diverse set of cellular processes.    Sites of ADAR modification exhibit sequence motif preferences and tend to be concentrated within a small subset of total RNAs.    Finally, editing regulates expression levels of target mRNAsand strongly correlates with alternative splicing.  
Genome-Wide Analysis of A-to-I RNA Editing via Single Molecule Sequencing in Drosophila