GSE161755
Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection [toxN_overexpression_RNAseq]
The purpose of this experiment was to characterize ToxN cleavage specificity and locations of cleavage genome-wide in E. coli following overexpression. Overall design: One replicate of +ToxN and empty vector samples after 5 minutes of induction (rRNA subtracted) in the presence of toxI leaky expression, and one replicate of +ToxN and empty vector samples after 10 minutes of induction without toxI leaky expression (rRNA subtracted)