DRP004559
Analysis of epigenetic modifications in the chicken cells
In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone H3 variant CENP-A. Although deposition of CENP-A-containing nucleosomes is critical for centromere specification, it remains unknown whether additional epigenetic features of centromeric chromatin are required for centromere specification and/or kinetochore assembly. To identify centromere-specific histone modifications, we examined histone modifications at chicken centromeres, which lack highly repetitive sequences using ChIP-seq analysis. Through these analyses, we will understand that detailed molecular mechanisms of the centromere specification and the kinetochore assembly.
In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone H3 variant CENP-A. Although deposition of CENP-A-containing nucleosomes is critical for centromere specification, it remains unknown whether additional epigenetic features of centromeric chromatin are required for centromere specification and/or kinetochore assembly. To identify centromere-specific histone modifications, we examined histone modifications at chicken centromeres, which lack highly repetitive sequences using ChIP-seq analysis. Through these analyses, we will understand that detailed molecular mechanisms of the centromere specification and the kinetochore assembly.