ena-STUDY-ROTHAMSTED RESEARCH-03-09-2018-09:52:11:877-96
Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactions
Deep sequencing of small RNA preparations from Zymoseptoria tritici isolate IPO323 cultured in vitro (in Czapek-Dox Broth, CDB) and those from the infected wheat cv. Bobwhite leaf tissues sampled at the four different time points corresponding to the four critical phases of Septoria tritici blotch disease development, namely 4 dpi (asymptomatic phase), 9 dpi (transition to necrotrophic stage), 13 dpi (necrotrophic phase), and 21 dpi (profuse asexual sporulation) was carried out on an Illumina HiSeq 2000 using 50 cycle single end reads. Deep sequencing of small RNA preparations from mock-inoculated wheat cv. Bobwhite leaves sampled at 4 dpi, 9 dpi, 13 dpi, and 21 dpi and from healthy, untreated 17 days old wheat cv. Bobwhite leaves was also carried out using the same sequencing technology.
Deep sequencing of small RNA preparations from Zymoseptoria tritici isolate IPO323 cultured in vitro (in Czapek-Dox Broth, CDB) and those from the infected wheat cv. Bobwhite leaf tissues sampled at the four different time points corresponding to the four critical phases of Septoria tritici blotch disease development, namely 4 dpi (asymptomatic phase), 9 dpi (transition to necrotrophic stage), 13 dpi (necrotrophic phase), and 21 dpi (profuse asexual sporulation) was carried out on an Illumina HiSeq 2000 using 50 cycle single end reads. Deep sequencing of small RNA preparations from mock-inoculated wheat cv. Bobwhite leaves sampled at 4 dpi, 9 dpi, 13 dpi, and 21 dpi and from healthy, untreated 17 days old wheat cv. Bobwhite leaves was also carried out using the same sequencing technology.