GSE133272
Effects of transcription on genome - nuclear lamina interactions: DamID-seq data
In mammalian nuclei, transcriptionally active genomic regions tend to localize to the interior of the nucleus while inactive regions are often located at at the nuclear lamina.  In this study we activated specific genes in lamina-associated domains by TALE-VP64 or CRISPRa-mediated activation. We also reduced transcription of individual genes by knockout of promoter/enhancer regions, or by insertion of a transcription termination sequence. In each case we  generated  genome-wide DamID maps to determine changes in nuclear lamina interactions, and in selected cases we also generated Repli-seq maps and/or RNAseq data. Overall design: Transcription was locally manipulated in mouse and human cell lines by using several techniques: random integration of a hPGK-EGFP expression cassette, TALE-VP64, CRISPRa, deletion of endogenous gene promoters or truncation of an endogenous transcript. Changes in 3D genome architecture were assesses by mapping lamina interaction with DamID.