GSE173859
Detect-seq on transfected HEK293T cells [Detect-seq data]
We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were applied to validate the results of Detect-seq. Overall design: We applied DdCBEs to generate on-target editings on mt-DNA to see DdCBEs' editing performance and evaluate their effects on the whole mitochondrial genome. This is the Detect-seq part. Detect-seq utilized a dU-mapping strategy to profile on-target and off-target editing events by DdCBE at the whole-genome level.