Neuronal transcriptome of Panulirus argus
Neuronal transcriptome of Panulirus argus
Transcritome analysis from the CNS,stomatogastric ganglion, and olfactory cluster from Panulirus argus. Total RNA was extracted and cDNA librarie were generated and sequenced using a 454 platform.
Panulirus argus , the spiny lobster is one species of lobster found on the Atlantic coast of North America. This species is important to have for a comparative approach involving arthropods.  However, there is very limited information about the molecular organization of its nervous system. Here, using pyrosequencing approach we performed analysis of transcripts expressed in the CNS,Stomatogastric ganglion (STG)and olfactory cluster of Panulirus. Specifically, we developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis.