GSE64327
Genome-wide analysis of chromatin occupancy of GATA1 and its short isoform GATA1s (ChIP-Seq)
We report ChIP-Seq data for GATA1 and the leukemia-associated short isoform GATA1s in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential.  We introduced HA-tagged GATA1 or GATA1s into G1ME cells via retroviral transduction.  The cells were crosslinked at 48h post-transduction, and an HA antibody was used for chromatin immunoprecipitation (ChIP).  ChIP and input samples were sequenced on Illumina GAII or GAIIx high-throughput sequencers.  The data reveal a deficiency of chromatin occupancy by GATA1s, especially at genes involved in erythrocyte differentiation and function. Overall design: Examinaton of chromatin occupancy of GATA1 and GATA1s  in G1ME cells cultured in TPO.