E-MTAB-10483
RNA-seq to investigate diurnal regulation of gene expression in Medicago truncatula Jester accession.
The experiment looks for diurnal-regulated genes in Medicago truncatula plants. Medicago truncatula Jester accession plants were entrained for18 days in long days (16h light:8h dark) at 24°C constant temperature. Samples for two biological replicates per time point were collected every 4h for 24h beginning at ZT0 (subjective dawn) until ZT20. Total RNA was extracted from ground tissue using an RNeasy RNA extraction kit (Qiagen). Extracted total RNA was DNAse-treated (Invitrogen). Total RNA (2-3ug) was dried down and preserved in Sigma-Aldrich RNAstable before being couriered to BGI Tech Solutions (HONGKONG) Co., Ltd (https://www.bgi.com/global). BGI Tech prepared and processed libraries for 20M PE100 reads using their DNBseq platform - BGISEQ-500.
The experiment looks for diurnal-regulated genes in Medicago truncatula plants. Medicago truncatula Jester accession plants were entrained for18 days in long days (16h light:8h dark) at 24°C constant temperature. Samples for two biological replicates per time point were collected every 4h for 24h beginning at ZT0 (subjective dawn) until ZT20. Total RNA was extracted from ground tissue using an RNeasy RNA extraction kit (Qiagen). Extracted total RNA was DNAse-treated (Invitrogen). Total RNA (2-3ug) was dried down and preserved in Sigma-Aldrich RNAstable before being couriered to BGI Tech Solutions (HONGKONG) Co., Ltd (https://www.bgi.com/global). BGI Tech prepared and processed libraries for 20M PE100 reads using their DNBseq platform - BGISEQ-500.