GSE113796
mRNA-Seq of mouse Mettl3 cKO and Fto cKO hippocampus after fear conditioning
N6-Methyladenosine (m6A) and N6,2'-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown.  Here, we investigated the effect of gene deletion of Mettl3, a m6A methyltransferase, and Fto, a m6A and m6Am demethlyase, induced in adulthood in excitatory neurons of the CA1 and CA3 in the hippocampus (Nex-CreERT2 Mettl3 or Fto cKO) on the transcriptome of CA1 and CA3 as well as the transcriptomic response of the CA1 and CA3 transcriptome to fear conditioning. Overall design: 5 biological replicates each for CA1/3 of Nex-CreERT2 Mettl3 wild type ("WT") and conditional knock-out mice ("cKO") or Nex-CreERT2 Fto WT and cKO, each at baseline("Box")  or 24 h after fear conditioning ("FC").