PRJNA832495
microbial_20201015214634767
DNA of rhizosphere soil samples (0.5 g) was extracted by using a FastDNA spin kit for soil (MB Biomedical LLC, Ohio, USA), and the spectrophotometry (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used to determine the quantity and quality of the DNA. Each of the DNA samples was amplified separately using the fusion primer pair 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAA-3') for bacteria. Preparing Library and sequencing used an Illumina MiSeq paired-end 300 bp protocol (Illumina, Inc., San Diego, CA, USA), and they were performed and finished in Biomarker Technologies (Beijing. China)