Screening_Zebrafish_for_CRISPR_induced_indels-sc-3099
Screening_Zebrafish_for_CRISPR_induced_indels
Zebrafish embryos were injected with CRISPR reagents, collected at 48hours and loci corresponding to the CRISPRs were amplified by PCR and libraries were made by the addition of tailed oligos in a second round of PCR. Samples were pooled to make a single barcoded library prior to submission.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Loci from CRISPR injected Zebrafish was amplified and sequenced to identify indels induced by the CRISPR/Cas9 system.