GSE106468
RNA-seq profiles of WT and Qk-KO corpus callosum and oligodendrocytes
This study is to analyze the transcriptomic profiles of Plp-CreERT2;QKLoxP/LoxP (Qk-KO) and Plp-CreERT2;QKLoxP/+ (WT) mice and WT and Qk-KO oligodendrocytes to determine the differentially expressed genes. Overall design: Plp-CreERT2;QKLoxP/LoxP (Qk-KO) and Plp-CreERT2;QKLoxP/+ (WT) mice were injected with tamoxifen at 8-week, and corpus callosum were collected 4 days after tamoxifen injection. WT or Qk-KO neural stem cells were differentiated in Neurobasal medium containing B-27, 2 mM GlutaMAX-I, 100 ng/ml insulin-like growth factor 1 (IGF-1), and 50 ng/ml 3,3',5-Triiodo-L-thyronine (T3). The total RNAs were extracted by Rnasey kit and sent for sequencing with llumina HiSeq2000 Sequencer.