GSE159457
Identification and characterization of C4 dicarboxylic acid transporters in Pichia kudriavzevii CY902 strain
RNA-seq libraries were constructed for three samples, including (I) ?URA3 strain grown in SD medium containing 2% w/v glucose(pH=5.0);(II)?URA3 strain grown in SD medium containing  2%w/v glucose and 2% w/v succinic acid(pH=5.0);(III)?URA3 strain grown in SD medium containing  2% w/v succinic acid(pH=5.0). For preparation of RNA samples, ?URA3 cells grown overnight were inoculated into 50 ml liquid Synthetic Dextrose (SD) medium with the initial inoculation amount of OD600= 0.1, and cultured for 24 hours to the logarithmic growth phase (OD600= 2-3). The cells were collected by centrifugation at 6000g for 5 minutes. After washing twice with phosphate buffer saline (PBS), they were inoculated into new 50 mL SD medium containing carbon sources of different combinations of glucose and succinic acid .The pH of the culture sample is adjusted to 5.0 with NaOH. After flask culturing at 30°C and 250 rpm for an additional three hours, the yeast cells were collected by centrifugation for total RNA isolation and Illumina RNA-seq library construction.  Total RNA for samples were isolated using TRIzol reagent (Invitrogen, Grand Island, USA), then used for high-throughput RNA sequencing. The 150-nt paired-end RNA-seq libraries were generated commercially at Novogene Biotechnology Co. Ltd (Tianjin, China) by using Illumina's Hiseq X Ten platform (Illumina, San Diego, USA). Each sample contains two biological replicates. Overall design: mRNA profiles of  ?URA3 strain grown in SD medium containing carbon sources of different combinations of glucose and succinic acid were generated by deep sequencing, using Illumina's Hiseq X Ten platform