ena-STUDY-THE ROSLIN INSTITUTE-02-01-2018-09:54:16:195-652
Frontal cortex of male piglets housed in barren and environmentally enriched conditions
This dataset comprises RNA-sequencing of the frontal cortex of male piglets housed either in barren (control) pens or pens with additional environmental enrichment. 8 weaner pigs (1:1 male:female ratio) were housed in each of 6 enriched pens, with litter, weight and sex-matched controls in the barren pens, i.e. a total of 24 pigs in each of the barren and enriched conditions. At 56 days of age (+/- 3 days) one male per pen was sedated at 1 hour from the introduction of the enrichment stimulus, and one male per pen at 4 hours post enrichment stimulus. From this total set of samples (6x barren 1 hour, 6x enrichment 1 hour, 6x barren 4 hour, 6x enrichment 4 hour), polyA+ RNA-seq libraries were prepared by Edinburgh Genomics and >30 million stand-specific 75bp paired-end reads were sequenced per sample, using the Illumina HiSeq 4000 sequencing platform.  These data are released under Fort Lauderdale principles, as confirmed in the Toronto Statement (Toronto International Data Release Workshop. Birney et al. 2009. Pre-publication data sharing. Nature 461:168-170). Data producers reserve the right to make the first publication of a global analysis of this data. If you are unsure if you are allowed to publish on this dataset, please contact alistair.lawrence@sruc.ac.uk or sarah.brown.ed.ac.uk to enquire.
This dataset comprises RNA-sequencing of the frontal cortex of male piglets housed either in barren (control) pens or pens with additional environmental enrichment. 8 weaner pigs (1:1 male:female ratio) were housed in each of 6 enriched pens, with litter, weight and sex-matched controls in the barren pens, i.e. a total of 24 pigs in each of the barren and enriched conditions. At 56 days of age (+/- 3 days) one male per pen was sedated at 1 hour from the introduction of the enrichment stimulus, and one male per pen at 4 hours post enrichment stimulus. From this total set of samples (6x barren 1 hour, 6x enrichment 1 hour, 6x barren 4 hour, 6x enrichment 4 hour), polyA+ RNA-seq libraries were prepared by Edinburgh Genomics and >30 million stand-specific 75bp paired-end reads were sequenced per sample, using the Illumina HiSeq 4000 sequencing platform.  These data are released under Fort Lauderdale principles, as confirmed in the Toronto Statement (Toronto International Data Release Workshop. Birney et al. 2009. Pre-publication data sharing. Nature 461:168-170). Data producers reserve the right to make the first publication of a global analysis of this data. If you are unsure if you are allowed to publish on this dataset, please contact alistair.lawrence@sruc.ac.uk or sarah.brown.ed.ac.uk to enquire.