PRJNA770144
ANG-induced tRNA cleavage in U2OS cells (tRNA fraction)
Angiogenin (ANG) has been reported to cleave 3'-CCA termini as well as anticodon loop of tRNAs in vitro. This study is aimed to confirm whether ANG can cleave 3'-CCA termini of tRNAs in vivo. In this study, we made the cDNA library using mature tRNA fraction (50-110 nt) from ANG-treated U2OS cells to examine what proportion of mature tRNAs were CCA-cleaved.