ena-STUDY-WAGENINGEN UNIVERSITY-07-08-2019-13:26:18:489-255
Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial microbial community diversity
This study is part of paper: "Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial microbial community diversity"  In this study, eight lab-wetlands systems in the shape of flat-plate Plant-MFC were constructed. Spartina anglica model plant was planted in the anode chamber of the Plant-MFC reactors. Here, four wetlands compositions with activated carbon and/or marine sediment functioning as anodes were investigated for their suitability as a bioanode in a Plant-MFC system.Four different anode compositions were used to fill the anode compartments (650ml). Plant-MFC 1 and Plant-MFC 2, this duplicate was named as AC100, were filled with 100% activated carbon (AC); Plant-MFC 3 and Plant-MFC 4, this duplicate was named as MS100, were only filled with marine sediment; Plant-MFC 5 and Plant-MFC 6, this duplicate was named as AC67, were filled with a mixture of 67 % AC and 33% marine sediment; and Plant-MFC 7 and Plant-MFC 8, this duplicate was named as AC33, were filled with a mixture of 33% AC and 67% marine sediment. The utilized AC is granular activated carbon PK 1-3(Cabot Norit Netherlands BV, with apparent density of 290 g/L)At the end of the experiment (day 190), about 3 ml biomass samples from anode components (mixture of marine sediment, AC and plant roots) were taken for DNA analysis. Samples were taken from the MS100 (Plant-MFC 3 & Plant-MFC 4) and the AC33 (Plant-MFC 7 & Plant-MFC 8). For every reactor, five biomass samples were collected. Biomass samples were taken from five different locations in the anode. These five sample locations were clustered in two zones: upper zone (until 5cm below the anode surface) and lower zone (from 5 until 20 cm below the anode surface). The upper zone(UZ) sample points were (A) UZ-AN (anode) and (C) UZ-CC (current collector). The lower zone sample points were (B) LZ-RO (roots), (D) LZ-AN (anode), and (E) LZ-CC (current collector). In the MS100 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained marine sediment that were attached on the current collector. While, in the AC33 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained AC and marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained AC and marine sediment that were attached on the current collector. In total 20 samples were collected. The samples were stored immediately in -80 Centigrade freezer after collection before the DNA sequencing was performed.Further detail information can be found in the above mentioned paper.
This study is part of paper: "Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial microbial community diversity"  In this study, eight lab-wetlands systems in the shape of flat-plate Plant-MFC were constructed. Spartina anglica model plant was planted in the anode chamber of the Plant-MFC reactors. Here, four wetlands compositions with activated carbon and/or marine sediment functioning as anodes were investigated for their suitability as a bioanode in a Plant-MFC system.Four different anode compositions were used to fill the anode compartments (650ml). Plant-MFC 1 and Plant-MFC 2, this duplicate was named as AC100, were filled with 100% activated carbon (AC); Plant-MFC 3 and Plant-MFC 4, this duplicate was named as MS100, were only filled with marine sediment; Plant-MFC 5 and Plant-MFC 6, this duplicate was named as AC67, were filled with a mixture of 67 % AC and 33% marine sediment; and Plant-MFC 7 and Plant-MFC 8, this duplicate was named as AC33, were filled with a mixture of 33% AC and 67% marine sediment. The utilized AC is granular activated carbon PK 1-3(Cabot Norit Netherlands BV, with apparent density of 290 g/L)At the end of the experiment (day 190), about 3 ml biomass samples from anode components (mixture of marine sediment, AC and plant roots) were taken for DNA analysis. Samples were taken from the MS100 (Plant-MFC 3 & Plant-MFC 4) and the AC33 (Plant-MFC 7 & Plant-MFC 8). For every reactor, five biomass samples were collected. Biomass samples were taken from five different locations in the anode. These five sample locations were clustered in two zones: upper zone (until 5cm below the anode surface) and lower zone (from 5 until 20 cm below the anode surface). The upper zone(UZ) sample points were (A) UZ-AN (anode) and (C) UZ-CC (current collector). The lower zone sample points were (B) LZ-RO (roots), (D) LZ-AN (anode), and (E) LZ-CC (current collector). In the MS100 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained marine sediment that were attached on the current collector. While, in the AC33 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained AC and marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained AC and marine sediment that were attached on the current collector. In total 20 samples were collected. The samples were stored immediately in -80 Centigrade freezer after collection before the DNA sequencing was performed.Further detail information can be found in the above mentioned paper.