ena-STUDY-CBMSO-13-01-2016-08:48:11:307-560
RNA-Seq (HEK93_Homo Sapiens)
The regulation of the heterogeneity of the transcriptome and proteome represents a key step in understanding the differences in diversity between organisms of similar genetic complexities. TIA1 and TIAR intracellular antigens have been involved in the regulation and / or modulation of gene expression in different aspects of RNA metabolism, including: 1) transcription, through its interaction with DNA and RNA polymerase II; 2) pre-mRNA alternative splicing, through the selection of atypical 5' splice sites; 3) the location, stability and / or translation of eukaryotic mRNA through interaction with the 5 'and 3' UTRs and 4) control of biological programs that are for cellular survival (inflammation, proliferation, apoptosis, stress or virus infections). Accordingly, our research hypothesis is that these proteins play an important role in controlling gene expression by adapting the human proteome and transcriptome expression and function in order to survive over aberrant situations compromising cellular homeostasis in pathophysiological conditions such as cellular stress, tumorigenesis or aging and age-related diseases. This project aims to define changes in the transcriptome and proteome associated with the gain of function of these proteins, as phenomenon that contrasts and prevents the development of proliferative aberrant cell phenotype associated with reduced expression of TIA1 and / or TIAR.
The regulation of the heterogeneity of the transcriptome and proteome represents a key step in understanding the differences in diversity between organisms of similar genetic complexities. TIA1 and TIAR intracellular antigens have been involved in the regulation and / or modulation of gene expression in different aspects of RNA metabolism, including: 1) transcription, through its interaction with DNA and RNA polymerase II; 2) pre-mRNA alternative splicing, through the selection of atypical 5' splice sites; 3) the location, stability and / or translation of eukaryotic mRNA through interaction with the 5 'and 3' UTRs and 4) control of biological programs that are for cellular survival (inflammation, proliferation, apoptosis, stress or virus infections). Accordingly, our research hypothesis is that these proteins play an important role in controlling gene expression by adapting the human proteome and transcriptome expression and function in order to survive over aberrant situations compromising cellular homeostasis in pathophysiological conditions such as cellular stress, tumorigenesis or aging and age-related diseases. This project aims to define changes in the transcriptome and proteome associated with the gain of function of these proteins, as phenomenon that contrasts and prevents the development of proliferative aberrant cell phenotype associated with reduced expression of TIA1 and / or TIAR.