DRP000009
Subsurface mine microbial mat metagenome
This is a metagenomic project to figure out the microbial community             flourished along with hot water stream in the Japanese subsurface mine. As a first step             of this project, we constructed a fosmid library from the DNA directly isolated from the             microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we             determined the paired-end sequence of all fosmid clones. Next, we classified all clones             in phylum level based on the information for the origin of the genes identified in the             paired-end sequence and then we selected 179 clones evenly according to the ratio of             each classified phylum to cover whole microbial community. These selected clones were             sequenced by shotgun sequencing method (Sanger). Moreover, we selected 158 fosmid clones             to increase the genomic information in the microbial mat and sequenced all the selected             clones at once by 454 sequencer.
This is a metagenomic project to figure out the microbial community             flourished along with hot water stream in the Japanese subsurface mine.