PRJNA715913
The rate and spectrum of EMS-induced germline mutations in the microcrustacean Daphnia: on the prospect of forward genetics
We demonstrate that EMS can be used to induce mutations into the Daphnia genome by describing the mutation rate and spectrum of Daphnia exposed to 10mM and 25mM EMS for 4 hours. We also show that mutations are induced into consecutive broods of a single treated female.