PRJNA453550
Systematic identification of factors mediating accelerated mRNA degradation in response to changes in environmental nitrogen.
This study used a 4-thiouracil labeling-chase to assay mRNA stability genome-wide (S288C) across a nitrogen upshift, then used yeast (S288C) knockout Barcode sequencing after FACS after mRNA FISH (BFF) to systematically identify mediators of rapid GAP1 mRNA repression.