GSE111213
Blastemal progenitors modulate immune signaling during early limb regeneration.
Blastema formation is a hallmark of limb regeneration that requires proliferation and migration of progenitors derived from many tissues to the amputation plane. To better understand the genetic programs that initiate limb regeneration, we reasoned that blastemal progenitors would be among early proliferating cells in the stump following amputation. Here we separately profiled dividing and non-dividing stump tissues, as well as the wound epidermis, during early axolotl limb regeneration to examine transcriptional programs of blastemal progenitors. We provide a description of the changes in gene expression specific to early dividing cells at the site of amputation, inclusive of progenitors for the regenerating limb. This work collectively  demonstrates differential suppression/activation of core developmental signaling pathways in subsets of the early regenerating limb and further suggests that interleukin-8 (il-8) signaling is important for regeneration. Overall design: To identify transcripts that were differentially expressed and enriched within early dividing cells during axolotl limb regeneration, we separately sequenced stump-derived 4N, stump-derived 2N, and wound epidermis at 0, 4, and 5 days post-amputation (dpa). We sequenced a total of 27 samples, 3 biological replicates per timepoint. These samples were multiplexed and sequenced on 1 Illumina Nextseq 500 run and also across 6 lanes on a Hiseq 2500.