PRJNA869721
TCR-Engine: Deep mutational scanning library selections
TnT cells reconstituted with DMS libraries and displaying restored TCR-CD3 surface expression were isolated by FACS, expanded and re-sorted based on binding to cognate peptide-MHC dextramer or activation following co-culture with cells displaying target antigen. Following FACS, deep sequencing was performed to identify TCR variants that were enriched across selection steps.