GSE131881
MBOAT7 loss of function in clear cell Renal Carcinoma (ccRCC)
We demonstrated an increase in MBOAT7 and downstream phosphatidylinositol products in ccRCC. This RNAseq was of two loss of function clones and wild-type in the Caki-1 cell model (ccRCC cell line). Overall design: We used CRISPR/Cas9 to generate knockout cell lines validated using Sanger and Western Blotting. The knockout clones were then RNA sequenced against a wild-type counterpart.