GSE113375
Conditional DICER1 knockout hESCs
We report the generation and characterization of DICER1-deficient hESCs. We uncover an unexpected requirement for DICER1 as well as essential pro-survival roles of members of the mir-302- 367 and mir-371- 373 clusters in hESCs. Our work provides a robust platform for interrogating microRNA function in hESC and differentiation. Overall design: For RNAsequencing, total RNA was isolated from Tra-1-60+ sorted cells from +DOX and -DOX conditions using the miRNeasy Mini Kit (Qiagen Cat. No. 217004), following manufacturer's guidelines. Three replicates were done for each sample.