GSE161442
The Iqgap3-Ras axis drives stem cell proliferation in the stomach corpus during homeostasis and repair [RNA-Seq]
Objective: Tissue stem cells are central regulators of organ homeostasis.  We looked for a protein that is exclusively expressed and functionally involved in stem cell activity in rapidly proliferating isthmus stem cells in the stomach corpus. Design: We uncovered the specific expression of Iqgap3 in proliferating isthmus stem cells through immunofluorescence and in situ hybridization.  We performed lineage tracing and transcriptomic analysis of Iqgap3+ isthmus stem cells with the Iqgap3-2A-tdTomato mouse model.  Depletion of Iqgap3 revealed its functional importance in maintenance and proliferation of stem cells.  We further studied Iqgap3 expression and the associated gene expression changes during tissue repair after tamoxifen-induced damage.  Immunohistochemistry revealed elevated expression of Iqgap3 in proliferating regions of gastric tumors from patient samples. Results: Iqgap3 is a highly specific marker of proliferating isthmus stem cells during homeostasis.  Iqgap3+ isthmus stem cells give rise to major cell types of the corpus unit.  Iqgap3 expression is essential for the maintenance of stem potential.  The Ras pathway is a critical partner of Iqgap3 in promoting strong proliferation in isthmus stem cells. The robust induction of Iqgap3 expression following tissue damage indicates an active role for Iqgap3 in tissue regeneration. Conclusion: IQGAP3 is a major regulator of stomach epithelial tissue homeostasis and repair.  The upregulation of IQGAP3 in gastric cancer suggests that IQGAP3 plays an important role in cancer cell proliferation. Overall design: RNA expression profile of stomach corpus 48 hr after HDT treated or untreated 6-8 weeks old mice. Total RNA was extracted from the corpus tissue.  Duplicate samples are shown.