GSE137431
Relief of tumor hypoxia unleashes the tumoricidal potential of neutrophils
Our objective was to identify the genes in tumor-associated neutrophils that are differentially expressed when uterine tumor-bearing "PRPL" mice are subjected to respiratory hyperoxia versus normoxia housing conditions. Six samples were spread across thw two housing conditions, each with 3 biological replicates.  Sequencing provided was 372 million total reads with an average of 81.9% of these reads aligning uniquely to the mouse genome. Reads uniquely mapped to known mRNAs were used to identify gene expression changes between housing conditions using DESeq2. We found that 423 protein-coding genes were differentially expressed (FDR<0.05, excluding genes whose normalized read counts were less than 50 across all six samples) in tumor-associated neutrophils from 4-wk old PRPL mice housed under hyperoxia versus normoxia conditions. Overall design: mRNA profiles of neutrophils sorted from uteri of 4-wk old PRPL mice (housed in normoxia or hyperoxia conditions for the last 10 days prior to sacrifice) were generated by next generation sequencing using Illumina HiSeq 4000.