PRJNA738318
RNA sequencing data for responses to drought stress and/or clubroot pathogen infection in developing seeds of Brassica napus
RNA-seq dataset consisting of 72 samples of immature Brassica napus seeds from plants grown under drought, infected with the pathogen Plasmodiophora brassicae, or a combination of both stresses in comparison to control growth conditions. RNA samples were sent to the BGI, Honk Kong (https://www.bgi.com), for library construction using the QuantSeq 3' mRNA-Seq Library Prep Kit REV (Lexogen) and sequencing using an Illumina HiSeq4000 platform that generated an average 9.38 M of 100 bp paired-end reads per sample. The dataset contains 67.6 Gb of transcriptome paired-end reads. These data are useful for the scientific community working on the impact of single or combined abiotic and biotic constraints on seed quality. RNA-seq data provides a key resource to unravel the transcriptomic programs that support the seed development under standard conditions or environmental stresses. The data will provide candidate genes including regulatory factors to predict seed quality under various conditions. Further analyses of the transcriptome dataset described here will provide useful information to accelerate the development of stress-resistant oilseed rape cultivars.