GSE117483
RNAseq in NMe wild type and NMe GATA site 1 and 2 homozygous mutant DN3 thymocytes
The NOTCH1-driven MYC enhancer (NMe) is essential for T cell development, transformation and the development of acute lymphoblastic leukemia. Here we analyze by RNAseq the gene expression profile of DN3 thymocytes from NMe wild type and NMe GATA site 1 and 2 (GS1+2) homozygous mutant mice. GSEA analysis show a significant enrichment of MYC signature genes, consistent with a deficient function of NMe in GATA site 1 and 2 homozygous mutant thymocytes. Overall design: RNAseq analysis of DN3 thymocytes in two genotypes and three samples per genotype