GSE129444
Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]
We inserted the inducible expression cassette of Runx1 into the Rosa26 locus of embryonic  stem  cells.  Based  on  a  modified  protocol  for  HEC  induction  from  PSC,  we  successfully generated iHEC Under the haematopoietic induction process (iR-HEC). Overall design: mRNA profiles of single hematopoietic endothelia cells (iR-iHEC). single iR-iHECs were sorted from PSC-derived cells for RNAseq analysis according to kit instruction of Discover-sc WTA Kit V2(Vazyme) and TruePrepTM  DNA Library Prep Kit V2 (Vazyme), using illumina HiSeq X Ten.