GSE158540
Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition [ChIP-seq]
We performed ChIP-seq ananlysis on HDAC1 in MiaPaCa2 using two different antibodies (Santa Cruz Biotechnologies sc-81598 HDAC1(10E2) and Invitrogen HDAC1 Polyclonal Antibody PA1-860), two non-targeting scrableled sgRNA controls and two replicates for each. Overall design: ChIP-seq data in both control and HDAC1 over expressed MiaPaCa2 cell lines were produced using standard ENCODE protocols ( https://www.encodeproject.org/documents/73c95206-fc02-41ea-93e0-a929a6939aaf/ )