Transcriptome_profiling_of_zebrafish_metabolic_mutants-sc-3445
Transcriptome_profiling_of_zebrafish_metabolic_mutants
Total RNA was extracted from zebrafish embryos collected for one or more alleles of various metabolic mutants. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Paired-end sequence data from the IlluminaHiSeq was prepared from RNA of mutant and wild type zebrafish larvae of metabolic mutants for transcriptome profiling.