GSE108535
Systematic Dissection of Sequence Elements Controlling s70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli
We built and tested a library of 10,898 s70 promoter variants consisting of all combinations of a set of eight -35 elements, eight -10 elements, three UP elements, eight spacers, and eight backgrounds. Using a Massively Parallel Reporter Assay, we measure expression of all promoters and evaluate how individual promoter elements contribute to expression. Overall design: We measure expression of all promoters and evaluate how individual promoter elements contribute to expression.