PRJNA487142
RNA-seq of wild-type and transgenic purple tomato fruits at mature green (MG) and breaker (BR+0) stage
Total RNA was extracted from MG and BR+0 stage fruits of wild type and transgenic purple tomato using an RNeasy Plant Mini Kit (Tiangen, China) according to the manufacturer's protocol. The centration and quality of the RNA were assayed using a Nano Drop Lite (Thermo Scientific). The cDNA libraries were constructed and then sequenced using a BGISEQ-500 System (BGI Inc.). Clean data were obtained after filtering and then mapped to the reference genome of S.lycopersicum in the Tomato SGN database (http://solgenomics.net/) with Bowtie2. The homogenized data were used to calculate gene expression levels with RSEM. The criteria for defining differentially expressed genes (DEGs) were fold change = 2.00 and P value = 0.05.