ena-STUDY-Institute for Chemistry and Biology of the Marine Environment-10-06-2021-09:32:48:420-773
Effects of antibiotics on soil microbial communities
Frequent fertilization of agricultural used land with liquid manure leads to an entry of veterinary pharmaceuticals into soils with the risk of affecting soil microbial communities. In this study, the effect of the antibiotics lincomycin (LIN) and sulfadimidine (SDM) on the microbial community of an agricultural soil was investigated conducting a column experiment. Soil filled columns were steadily treated with LIN or SDM for three weeks. Subsequently, the resulting bacterial communities were cultivated with higher doses of the pharmaceuticals. The bacteria of the soil and the cultivated communities were analysed by Illumina sequencing. Abiotic factors like pH, oxygen saturation and ion concentrations were examined throughout the experiment. Sequence analysis showed, that both the continuous input and the one-time addition of higher doses of LIN or SDM led to changes within community structure and diversity. A significant increase in nitrate reduction in the treatments with LIN and SDM was correlated by a strong growth of bacteria of the genus Rhodanobacter. This leads to the conclusion, that an input of antibiotic residuals into soils can induce shifts within the microbial community structure, fostering the growth of specific bacteria and thus affecting the ecological function of the soil bacterial community.
Frequent fertilization of agricultural used land with liquid manure leads to an entry of veterinary pharmaceuticals into soils with the risk of affecting soil microbial communities. In this study, the effect of the antibiotics lincomycin (LIN) and sulfadimidine (SDM) on the microbial community of an agricultural soil was investigated conducting a column experiment. Soil filled columns were steadily treated with LIN or SDM for three weeks. Subsequently, the resulting bacterial communities were cultivated with higher doses of the pharmaceuticals. The bacteria of the soil and the cultivated communities were analysed by Illumina sequencing. Abiotic factors like pH, oxygen saturation and ion concentrations were examined throughout the experiment. Sequence analysis showed, that both the continuous input and the one-time addition of higher doses of LIN or SDM led to changes within community structure and diversity. A significant increase in nitrate reduction in the treatments with LIN and SDM was correlated by a strong growth of bacteria of the genus Rhodanobacter. This leads to the conclusion, that an input of antibiotic residuals into soils can induce shifts within the microbial community structure, fostering the growth of specific bacteria and thus affecting the ecological function of the soil bacterial community.