GSE201377
Dual-transcriptomic analysis of Nicotiana benthamiana and two compatible Pseudomonas strains
We sought to compare and contrast plant host and bacterial transcriptional changes during compatible infections that cause disease (albeit within different symptoms). We investigated the infection by the two Pseudomonas syringae sensu lato strains P. syringae pv. syringae B728a (Psy) and P. amygdali pv. tabaci 11528 (Pta) of Nicotiana benthamiana at an early time point post inoculation to understand how a plant host responds to two related bacteria with different infection strategies. Plant and bacterial transcriptomes were analyzed prior to and five hours post inoculation. Overall design: RNA was taken from six Nicotiana benthamiana plants before and five hours after syringe inoculation with Psy or Pta for assessing the plant transcriptome. For the bacterial transcriptomes, bacteria growing on King's B medium and from the leaf intercellular wash fluid five hours post inoculation were used for RNA isolation. For each organism and condition there are 3 replicates. To determine whether bacterial transcriptomes were impacted by pelleting before RNA collection, the Psy B728a has a pelleted (P) and not-pelleted (N) set of samples for the in vitro timepoint.