PRJNA837373
Transcriptome changes following treatment of three human cancer cell lines (U-2 OS, NCI-H460, A375) with actinomycin D and nutlin-3a.
Actinomycin D and nutlin-3a act synergistically in activation of p53 transcription factor and, consequently, synergistically activate a subset of p53-regulated genes. The goal was to compare transcriptome changes of cell lines derived from three different cancers. The cells grew in control conditions or were exposed for 30 hours to 5nM actinomycin D and 5 uM nutlin-3a.