GSE126969
Comparative bulk RNAseq analysis of CXCL12-abundant reticular (CAR) cells, fetal perichondrium-derived CAR cells and chondrocyte-derived CAR cells.
We revealed the unique molecular signature characterizing fetal perichondrium-derived and chondrocyte-derived CAR cells Overall design: GFP+tdTomato+ cells were FACS-isolated from postnatal day 21 (P21) femurs of Cxcl12-GFP; Hes1-creER; R26R-tdTomato and Cxcl12-GFP; Fgfr3-iCreER; R26R-tdTomato mice, both pulsed with a single dose of tamoxifen at embryonic day 12.5 (E12.5).  mRNA were amplifed by SMARTer mRNA-seq kit (Clonetech/Takara) before deep-sequenced by Illumina HiSeq4000.