BarSoS method development
Barcoded Solexa sequencing, a highthroughput and cost effective method to study microbial diversity
We developed barcoded solexa sequencing of 16S rRNA V6 tags to study microbial diversity. A total of 65 samples were determined, in which 52 were bacterial V6 tags and 13 were archaeal V6 tags. The DNA samples were prepared from freshwater sediment, mangrove sediment and marine sediment taken from Pearl River Delta, China.