Sequencing of candidate positively-selected regions in the human genome-sc-2010-04-27
Sequencing of candidate positively-selected regions in the human genome
About 3Mb of sequence from 57 regions (20-500kb each) of the human genome were enriched by NimbleGen custom oligo-tilling array pulldown and/or solution pulldown technology. The regions were then sequenced using paired-end reads (35bp, 54bp or 76bp) on the Illumina sequencing platform. SNPs were called by the Sanger variation informatics pipeline (SAM/BAM) and population genetic analyses are being carried out on these data.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The project aims to investigate evolutionarily-interesting regions of the human genome. These often show signals of positive natural selection. Resequencing the same region of DNA in multiple humans provides a complete catalogue of the variants it contains and their distribution in the sample, and is the best basis for characterising positive selection. We have chosen a number of regions of the genome that are either of known evolutionary interest, or that show a possible signal of selection that we want to investigate further, along with some controls, and are resequencing them in a population sample of 30. Our analyses will extract information on neutral and selective aspects of the evolutionary history of these regions.