PRJNA865105
hmC-seq of human post-mortem brain (bulk, NeuN+, NeuN-)
We separated the neuronal (NeuN+) and non-neuronal (NeuN-) nuclei from prefrontal cortex cells of an 84 years old male without history of neurologic or psychiatric incident. After extraction of genomic DNA, we performed hmC-Seq. In hmC-Seq, we identified hmC-enriched genomic DNA region by utilizing the glucosylation of hmC with a B-glucosyltransferase, followed by next generation sequencing.