GSE117940
ATAC-seq of MCF-7 cells post ligand treatment
Goal: study the impact of estrogen receptor ligands on chromatin accessibility in MCF-7 cells Methods: Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-Seq) Results: Ligand 4-OH tamoxifen, a selective ER modulator (SERM), significantly alters chromatin accessibility and partially mimics the effect of natural ligand E2 on chromatin accessibility in MCF-7 breast cells. Selective ER degraders (SERD) fulvestrant and GDC-0927 on the other hand have very little impact on chromatin accessibility. Overall design: Triplicates results of ATAC-Seq of MCF-7 cells treated with five different estrogen receptor ligands or DMSO for 45 minutes