PRJNA444104
Light-Activated Cell Identification and Sorting, LACIS, for Selection of Edited Clones on a Nanofluidic Device
Sequence data supporting publication of CRISPR gene-edit assessment on Berkeley Lights Inc. technology.Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious and low-yield. Here, we establish a method to enable clonal isolation, selection and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. Briefly, after electroporation of primary T cells with CXCR4-targeting Cas9 ribonucleoproteins, single T cells are isolated on a chip and expanded into colonies. Phenotypic consequences of editing are rapidly assessed on-chip with cell-surface staining for CXCR4. Furthermore, individual colonies are identified based on their specific genotype. Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. Using this method, single-clone editing efficiencies, including the rate of mono- and bi-allelic indels or precise nucleotide replacements, can be assessed within 10 days from Cas9-RNP introduction in cells.