ena-STUDY-ROTHAMSTED RESEARCH-28-07-2017-15:03:06:794-1112
Small RNA sequencing of Foxtail mosaic virus-infected wheat after 7 serial passages
Freeze-dried FoMV PV139 infected Sorghum bicolor (Asgrow XP6105) leaves were used as a source of inoculum to infect three different wheat cultivars (Chinese Spring, Riband, and Bobwhite). Systemically infected leaves showing moderate mosaic symptoms where collected at 14-21 days post-inoculation (dpi) and used as a source of inoculum for rub-inoculation of new batches of wheat plants. This passaging sequence was repeated seven times. Systemic leaves of three cv. Riband and three cv. Chinese Spring FoMV-infected plants were collected at 26 days after the seventh passaging and pooled. Total RNA samples from two independent pools of leaves were extracted using Trizol and used for small RNA-seq on an Illumina HiSeq 2500 (1x50 bp) platform.
Freeze-dried FoMV PV139 infected Sorghum bicolor (Asgrow XP6105) leaves were used as a source of inoculum to infect three different wheat cultivars (Chinese Spring, Riband, and Bobwhite). Systemically infected leaves showing moderate mosaic symptoms where collected at 14-21 days post-inoculation (dpi) and used as a source of inoculum for rub-inoculation of new batches of wheat plants. This passaging sequence was repeated seven times. Systemic leaves of three cv. Riband and three cv. Chinese Spring FoMV-infected plants were collected at 26 days after the seventh passaging and pooled. Total RNA samples from two independent pools of leaves were extracted using Trizol and used for small RNA-seq on an Illumina HiSeq 2500 (1x50 bp) platform.