Genome_re_sequencing_of_Plasmodium_chabaudi_clones__-sc-2011-04-20T12:55:18Z-363
Genome_re_sequencing_of_Plasmodium_chabaudi_clones__
As part of P. chabaudi chabaudi AS genome sequencing project, we are sequencing (with Illumina technology platform) four cloned lines of P. chabaudi parasites with defined and varying phenotypes. P. chabaudi chabaudi  AJ is a line of P. chabaudi chabaudi  obtained from an isolate collected from Thamnomys rutilans (otherwise known as thicket rats or tree rats) caught in the Central African Republic and sent to Edinburgh in 1969. AJ was subsequently cloned by Prof. David Walliker in Edinburgh (Walliker et al., 1975, Parasitology 70, 19-24 and Carter and Walliker, 1975, Ann. Trop. Med. Parasitol. 69, 187-196). P. chabaudi chabaudi CB is a line of P. chabaudi chabaudi obtained from an isolate collected from Thamnomys rutilans from La Maboke field station Central African Republic that was sent to Edinburgh in 1970. It was subsequently cloned by Prof. David Walliker in Edinburgh (Walliker et al., 1975, Parasitology 70, 19-24 and Carter and Walliker, 1975, Ann. Trop. Med. Parasitol. 69, 187-196). P. chabaudi chabaudi  DS is a virulent line of P. chabaudi adami (otherwise known as 408XZ). The isolate came from Thamnomys rutilans from Congo-Brazzaville (1972). It contained two species, namely P.chabaudi adami and P.vinkei lentum.  The isolate was cloned by Prof. David Walliker and the cloned line sent to WTSI is designated DS and is P.c. adami (Carter and Walliker (1976) An Parasitol Hum Comp. 51(6), 637-646). P. chabaudi chabaudi DK is a non-virulent line of P.chabaudi adami (otherwise known as 556KA). It was isolated from Congo-Brazzaville and sent to Edinburgh in 1971 where it was cloned by Prof. David Walliker (Carter and Walliker (1976) An Parasitol Hum Comp. 51(6), 637-646). All the cloned lines being sequenced are pyrimethamine sensitive and are genetically distinct from each other.  AJ, AS and CB all induce reciprocal strain-specific protective immunity in their rodent hosts. AS (genome sequencing line) AJ and CB strains of P.c. chabaudi have been used in Linkage Group Selection experiments to identify the target antigens of strain-specific protective immunity against malaria. Two independent genetic crosses between AS and CB strains and AJ and CB strain combinations were conducted in Edinburgh and progeny parasites from these crosses were selected in mice made immune to AJ, AS or CB by natural infection with blood stage parasites, followed by drug cure. In challenge infections in AJ, AS or CB-immune mice with the cross progeny parasites, LGS identified the Merozoite Surface protein 1 (MSP-1) as the principal target of strain-specific protective immunity. In the AJxCB cross progeny selected in AJ or CB-immune mice, however, there is some evidence for the potential involvement of other parasites genes in strain-specific immunity (but to a much lesser extent than MSP-1). We aim to generate PCR-free genomic Solexa reads (1 Illumina lane each,  typically producing 60-80X coverage of the genome) and produce a catalogue of genomic differences between these strains and the genome sequencing strain (AS). . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Illumina sequencing of cloned Plasmodium chabaudi lines with distinct biological phenotypes to study genome variation. Strains (i.e. cloned lines) to be sequenced are P. chabaudi chabaudi AJ, P. chabaudi chabaudi CB, P. chabaudi chabaudi DS and P. chabaudi chabaudi DK.