GSE159118
Transcriptome and epigenomics characteristics of LPS induced CD127 expressing human monocytes
We performed the transcriptomic and epigenetic analysis for both CD127high and CD127low monocytes under LPS stimulation, and RNA-seq and ATAC-seq were performed accordingly for this purpose. Overall design: PBMC were isolated from buffy coat of healthy blood donors by Ficoll gradients, and CD14+ monocytes were isolated from PBMC with anti-CD14 magnetic beads (Miltenyi Biotec). CD14+ monocytes were treated with LPS for 6 h and then were collected for FACS-based sorting of CD127high and CD127low monocytes. Sorted cells were either directly used for ATAC-seq or TRIzol lysed for RNA extraction.