GSE122356
Characterization of the DNA methylome in murine Müller glia
We investigated the epigenetic plasticity of adult (postnatal day (P) 28) murine Müller glia using whole-genome bisulfite sequencing (WGBS) Overall design: To study DNA methylation in the adult Müller glia, we isolated Glast+ cells from P28 retinas (Glast/Slc1a3 is a marker of Müller glia precursors and Müller glia in the retina). Two WGBS libraries were prepared using DNA from two independent samples containing 150,000–200,000 cells. The WGBS libraries were sequenced and bisulfite sequence reads were aligned using the Bismark software package. Global methylation level in adult mouse Müller glia was measured and percentages of methylation of CpG, CHG, and CHH contexts (where H is A, C or T) were identified.