ena-STUDY-INSTITUTO DE BIOTECNOLOGIA UNIVERSIDAD NACIONAL DE-12-10-2017-17:11:54:470-70
Transcriptome analysis of the venom gland of Thorellius atrox by RNA-seq
The venom glands from the telsons of four specimens of the Mexican scorpion Thorellius atrox were dissected and the total RNA was extracted. A cDNA library was constructed and subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 44,049,844 reads were obtained by the RNA-seq procedure. The Trinity assembly resulted in a total of 129,950 transcripts, of which 20,851 were successfully annotated by Trinotate. One hundred and sixty transcripts were identified potentially coding for scorpion venom components.
The venom glands from the telsons of four specimens of the Mexican scorpion Thorellius atrox were dissected and the total RNA was extracted. A cDNA library was constructed and subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 44,049,844 reads were obtained by the RNA-seq procedure. The Trinity assembly resulted in a total of 129,950 transcripts, of which 20,851 were successfully annotated by Trinotate. One hundred and sixty transcripts were identified potentially coding for scorpion venom components.