GSE116579
Newly identified Aspergillus nidulans PKA targets play a role in carbon-catabolite repression
A transcriptomic assessment of a kinase deletion mutant (?pkaA) and a control strain revealed many cellular activities this kinase is impacting. Studies were conducted in the model filamentous fungi, Aspergillus nidulans¸ under rich growth conditions. PkaA is a known kinase that mediates a wide range of processes including: nutrient sensing, stress responses, regulation of metabolism, as well as development and pathogenicity. Here, fungi were grown in rich media containing glucose. The comparison between the control strain and the deletion mutant transcript levels revealed previously unknown targets, such as the transcription factor CreA, the main repressor responsible for carbon catabolite repression (CCR). Overall design: A control strain and deletion mutant grown in rich media (2 biological replicates and 2 technical replicates) were analyzed to determine unknown functions and targest of PkaA using RNA-seq.