PRJNA561035
AAVS locus site identification in AAVS1-transgenic mice
Localization of the AAVS1 locus in AAVS transgenic mice:Inverse PCR (iPCR) for integration site analysis requires the knowledge of the AAVS1 locus localization in the genome of AAVS1/CD46-transgenic mice. To determine this, we used a targeted locus amplification (TLA)/PCR technology that involves thecrosslinking of physically proximal sequences. The TLA data obtainedfrom bone marrow cells from AAVS1/CD46-tg mice were then aligned with a reference mouse genome (mm10).TLA results indicate that the AAVS1 transgene is integrated into chromosome 14 at the location(Chr14:110443871-110461834), accompanied by a 18Kb deletion of mouse genome sequence.