PRJNA545390
identification of lactobacillus plantarum isolated from traditional fermented milk
The potential of oxalate degrading probiotic strains play essential role in management of kidney stone disease. In this study, molecular analysis of oxalate degrading lactic acid bacterial genome revealed a cluster of genes encoding putative formyl coenzyme A transferase (frc) and oxalyl coenzyme A decarboxylase (oxc) homologues, possibly involved in oxalate catabolism. Two method of detection and identification of oxc gene of bacterial strains were isolated from human fecal sample ( collected from healtheir voleenters) and traditional fermented milk . Genomic DNA was isolated from selected probiotic potential bacterial culture and specific 16S rDNA was amplified by polymerase chain reaction (PCR) followed by sequencing. Total nineteen Lactic acid bacterial strains were screened by PCR for the presence of oxc genes using gene specific primers. Oxalate-degrading bacteria were identified by comparing the sequences of 16S rDNA. Oxc gene was detected from four enterococcus hirae strain, one enterococcus fecalis strain and one Lactobacillus helveticus. It is very common to have lactobacilli in human gut and are included in fermented dairy products can be our daily routine diets