GSE160347
Transcriptomic study of Bacillus subtilis lacking seven extracytoplasmic function (ECF) s factors
Bacillus subtilis encodes seven extracytoplasmic function (ECF) s factors, sM, sW, sX, sY, sZ, sV and sYlaC, that mediate response to cell envelop-active compounds. In this study, we aimed to define the transcriptomic differences between B. subtilis W168 wild type and the derivative 168 ?7ECF mutant lacking seven ECF sigma factors under unstressed condition, which allows to identify the genes under control of these seven ECF sigma factors. 168 ?7ECF mutant was as well treated with cell wall-targeting antibiotics, vancomycin and tunicamycin, in order to unravel the genes that respond to these two compounds, however are not under the seven ECF sigma regulon. Overall design: B. subtilis W168 ?7ECF mutant at exponential phase of growth were treated with cell envelop-targeting compounds, vancomycin and tunicamycin, respectively, or remained untreated. The wild type B. subtilis W168 untreated undergoing the same growth procedure was used as control. With 10 min of induction, all samples were processed to undergo total RNA extraction, cDNA library preparation and then high-throughput RNA sequencing. The resulting sequencing raw data were analyzed in silico to reveal the differential gene expression in different treatments relative to non-treatment samples and control samples.