First Ion Torrent Human Genome
Ion Torrent Sequencing Data March 2011
We describe a DNA sequencing technology                 in which scalable, low cost semiconductor manufacturing techniques are                 used to                 make an integrated circuit able to directly perform non-optical DNA                 sequencing                 of genomes. Sequence data are obtained by directly sensing the ions                 produced by                 template-directed DNA polymerase synthesis using all-natural nucleotides on                 this massively parallel semiconductor-sensing device or ion chip.  The ion                 chip contains ion-sensitive,                 field-effect transistor-based sensors in perfect register with 1.2 million                 wells, which provide confinement and allow parallel, simultaneous                 detection of                 independent sequencing reactions.  The                 use of the most widely used technology for constructing integrated                 circuits,                 the CMOS process, allows for low cost, large-scale production and scaling                 of                 the device to higher densities and larger array sizes. We show the                 performance                 of the system by sequencing three bacterial genomes, and its robustness and                 scalability by producing ion chips with up to 10 times as many sensors and                 sequencing a human genome