Albimanus_EST_454
Transcriptome of the adult female malaria mosquito vector Anopheles albimanus
We combined Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. This record includes the 454 sequencing data of dorsal vessel, midgut and cuticular epithelium. Illumina data from midgut are described separately in SRA051893. Sanger sequencing data are accessible at dbEST (EV406110.1 - EV410194.1). Cite: Martinez-Barnetche, et al. Transcriptome of the adult female malaria mosquito vector Anopheles albimanus. Submitted: BMC Genomics. April, 2012.
Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas.  The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 million years.  To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control we sequenced the transcriptome of the An. albimanus adult female.  An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects (http://funcgen.vectorbase.org/RNAseq/Anopheles_albimanus/INSP/v2/). Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors.  The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/).