GSE124001
Transcriptome analysis of SH-SY5Y cells after knockdown of circSLC45A4
We perturbed the expression levels of circSLC45A4 in the human neuroblastoma cell line (SH-SY5Y) by RNAi/siRNAs. 96 h post-knockdown the cells were harvested, RNA extracted with Trizol and polyA+ libraries were generated with Illumina's TruSeq RNA Library Prep Kit v2. We find many significant changes in the transcriptomic profile after knockdown of circSLC45A4 and the induction of many genes connected to nervous system development, neuron projection development, synapse organization and cell adhesion. Overall design: Examination of 3 different siRNAs against circSLC45A4 vs. 1 scrambled siRNAs, 2 biological replicates per siRNA.