GSE175714
YBX1 mediates translation of oncogenic transcripts to control cell competition in AML [RNA-seq polysomes]
RNA-seq followed by digital gene expression analyses we assessed genes that change upon CRISPR-Cas9-mediated knockout of YBX1 in myeloid leukemia cells (MOLM13 cell line). Overall design: Gene expression in total mRNA and mRNA from purified polysomal fractions was assessed in cells harboring a YBX1-knockout using a pre-validated CRISPR-Cas9 guide-RNAs and a non-targeting guide as control at 14 days after lentiviral transduction.