GSE201528
RNA-seq of EBF1E271A-transduced progenitors
Gene expression analysis in Ebf1-/- lymphocyte progenitors complemented with EBF1wt or EBF1E271A and co-cultured with OP9-DL1 feeder cells. Overall design: Ebf1-/- c-kit+ lymphocyte progenitors isolated from fetal liver were transduced with EBF1wt- or EBF1E271A-expressing retroviruses, sorted for retroviral expression and co-cultured with OP9-DL1 feeder cells. For RNA-seq analysis, either GFP+CD19- progenitors were sorted after 7 days, or GFP+ progenitors were sorted after 3 days. 3 independent experiments were analyzed for each progenitor population.