GSE113602
Single-cell RNA-seq of sorted CD8+ T cells from B16 melanoma tumors
The response of naive CD8 T cells to acute infections results in the generation of defined yet heterogeneous pools of effector and memory cells. In contrast, the complexity of tumor infiltrating CD8 T cell states is still not fully elucidated. To delineate the landscape of tumor infiltrating CD8 T cells in an unbiased manner, we performed single-cell (full-length) RNA-Seq of CD8 T cells infiltrating mouse B16 melanomas. Our analysis of cell heterogeneity revealed the presence of multiple distinct CD8 T cell subtypes including exhausted, terminal effector, naive and memory-like CD8 T cells, as well as novel intermediate states. TCR reconstruction and pseudotime ordering enabled us to follow differentiation of single clones and suggested distinct trajectories towards T-cell exhaustion. Overall design: single-cell (full-length) RNA-Seq of CD8 T cells infiltrating mouse B16 melanomas Please note that [1] there are 192 samples included in the study, and the processed data was generated from  the 135 quality filtered samples (filetering parameters are provided in the data processing steps) [2] The cell subtype information for each sample is described and provided in the associated publication.