DRP004521
Effect of IBS004735, a TAZ activator, on C2C12 myodifferentiation in vitro
Expression of transcriptional co-activator with PDZ-binding motif (TAZ) is enhanced during skeletal muscle repair after damage. Enforced expression of TAZ increases muscle mass. These findings implicate that TAZ activators are beneficial to prevent and treat muscle atrophy. We searched for chemical compounds that empower human mammalian epithelial MCF10A cells expressing TAZ to form spheres in defined medium under floating condition. We further selected the compounds to promote myogenesis in mouse myoblast C2C12 cells and obtained several TAZ activators including IBS004735. To obtain better understanding of IBS004735's mode of action, we performed Cap Analysis of Gene Expression (CAGE)-seq using samples of IBS004735-treated differentiating C2C12 cells.