Pilot_for_RNAseq_investigating_the_transcriptome_of_early_infective_larvae_stage_of_Trichuris_muris-sc-4023
Pilot_for_RNAseq_investigating_the_transcriptome_of_early_infective_larvae_stage_of_Trichuris_muris
Whipworms (Trichuris trichuira) live preferentially in the cecum of their hosts where they tunnel through epithelial cells and cause inflammation potentially resulting in colitis. Despite extensive research, the role of whipworm early interactions with host intestinal epithelial cells in triggering parasite establishment or expulsion remains unclear. The goal of this project is to investigate novel interactions of whipworms with the host intestinal epithelia during the first events of infection.  This pilot study will test out SMARTseq2 + Illumina sequencing approach on the limited RNA available from our samples. If successful, the data will be used as the first replicate in a larger study.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
RNA-seq analysis of the early larvae and single cell intestinal epithelial cells of infected animals to further understand the murine whipworm â€“ host intestinal epithelial early interaction