Sequencing_of_the_gibbon_Y_chromosome-sc-4288
Sequencing_of_the_gibbon_Y_chromosome
To sequence the gibbon Y chromosome on the 10X Genomics platform, a pool of two 10x genomics chromium genome libraries (each 4 sub samples as normal) will be provided, with one library derived from flow-sorted Y chromosomes and the other from genomic DNA.  A single HiseqX lane will be enough if sequencign is successful.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
To understand the evolution of Y chromosomes of human and other primates, the  Y chromosomes of  two great  apes (i.e., gorilla & chimpanzee) and  one Old Wold monkey (i.e., rehesus macaque) have benn sequencesd using  the traditional clone-based Sanger sequencing method.   However, the  Y chromosome from the lesser apes  (gibbons), which are placed on the middle branch between the great apes and Old World monkeys in the tree of Primates and thus are critical to the  understanding of  the evolution of primate Y chromosomes,  has not been sequenced yet.  To fill this knowledge gap, we aim to sequence the gibbon Y chromosme  by taking advantage of the long-read capacity offered by the novel third generation sequencing methods such as the 10x Genomics and PacBio etc.