PRJNA836290
Transcriptome analysis of UFMylation gene knockouted-MDA-MB-231
To investagate the gene expression profiling of dysUFMylation in breast cancer, we constructed UFL1 and UFM1 konckouted-MDA-MB-231 using CRISPR-Cas9 and couducted RNAseq. mRNA were isolated and purified by using RNeasy Plus Mini Kit (Qiagen). Samples were prepared from three biological replicates.