GSE125397
mRNA polyadenylation profile analysis in control and U1 AMO treated HeLa cells
To globally assess the effect of polyadenylation site (PAS) usage upon functional knockdown of U1 snRNP, we carried out PAS-seq analysis with control and U1 AMO-treated HeLa cells using the QuantSeq Rev 3' mRNA sequencing library prep kit (Cat. 016-24). Overall design: For each sample (U1 ASO or control ASO treatment), we have 2 biological replicates in total we have 4 samples. Significant PAS profile change is expected to be detected between U1 and control samples.