ena-STUDY-INNER MONGOLIA AGRICULTURAL UNIVERSITY-10-06-2018-08:54:13:254-3793
Revealing of the sheep rumen fluid microbiome correlated with average daily gain in feeding Allium Mongolicum regel extracts by 16S rDNA profiling
Plant extracts could affect the rumen microbiome and the average daily gain (ADG)  in ruminants, and the association study between rumen microbiome and ruminants' growth performance probably serve as a faundation for improving the growth. Thus, it is necessary to conduct research on the correlation between the rumen microbiome and ADG in sheep. The objectives of the study were to investigate the effects of Allium Mongolicum regel extracts on the rumen microbiome and ADG, and to conduct a association study betweem rumen microbiome and ADG in sheep. Using completely randomized experimental design, a total of fourty eight sheep with similar body weight and age were assigned to four groups: sheep were fed basal diet was control group (CK), sheep were fed basic diets with product of Allium Mongolicum Regel powder extract (PAM), sheep were fed basic diets with 10g/head/day Allium Mongolicum Regel powder (AM) and the sheep were fed basic diets with residual of Allium Mongolicum Regel extract (RAM). Illumina HiSeq sequencing was used to investigate the profile changes of rumen microbiome and the association with the ADG was analyzed by the Spearman correlation analysis. The core dominant taxa were Bacteroidetes, Firmicutes and Proteobacteria, but relative to the CK, it showed that the dominant phyla was Tenericutes in PAM, AM and RAM groups and Fibrobacters in RAM group. Tenericutes (?=0.5021, p=0.0124) and Mollicutes (?=0.5021, p=0.0124) were positively correlated with ADG. but their relative abundances were not significantly different among four groups. Within Mollicutes, Anaeroplasmatales (?=0.5458, p=0.0058) and Anaeroplasmatoceae (?=0.5458, p=0.0058) were extrem significantly positively correlated with ADG and the relative abundances were significantly different: highest in RAM, followed by AM. The main negatively correlated bacteria were Saccharibacteria (?=-0.4762, p=0.0187), unidentified__Saccharibacteria (?=-0.4739, p=0.0193) class, order and family level, and their relative abundances were significantly different: the highest in CK and PAM. Betaproteobacteria (?=-0.5669, p=0.0039) was extreme significantly negatively correlated with ADG, and its relative abundance was significantly different: the highest in CK. In conclusion, this study provides information on the association between rumen microbiome and ADG in feeding Allium Mongolicum regel extracts in sheep, which could reveal the integrative information of rumen function and lead to an improvement in ruminant growth.
Plant extracts could affect the rumen microbiome and the average daily gain (ADG)  in ruminants, and the association study between rumen microbiome and ruminants' growth performance probably serve as a faundation for improving the growth. Thus, it is necessary to conduct research on the correlation between the rumen microbiome and ADG in sheep. The objectives of the study were to investigate the effects of Allium Mongolicum regel extracts on the rumen microbiome and ADG, and to conduct a association study betweem rumen microbiome and ADG in sheep. Using completely randomized experimental design, a total of fourty eight sheep with similar body weight and age were assigned to four groups: sheep were fed basal diet was control group (CK), sheep were fed basic diets with product of Allium Mongolicum Regel powder extract (PAM), sheep were fed basic diets with 10g/head/day Allium Mongolicum Regel powder (AM) and the sheep were fed basic diets with residual of Allium Mongolicum Regel extract (RAM). Illumina HiSeq sequencing was used to investigate the profile changes of rumen microbiome and the association with the ADG was analyzed by the Spearman correlation analysis. The core dominant taxa were Bacteroidetes, Firmicutes and Proteobacteria, but relative to the CK, it showed that the dominant phyla was Tenericutes in PAM, AM and RAM groups and Fibrobacters in RAM group. Tenericutes (?=0.5021, p=0.0124) and Mollicutes (?=0.5021, p=0.0124) were positively correlated with ADG. but their relative abundances were not significantly different among four groups. Within Mollicutes, Anaeroplasmatales (?=0.5458, p=0.0058) and Anaeroplasmatoceae (?=0.5458, p=0.0058) were extrem significantly positively correlated with ADG and the relative abundances were significantly different: highest in RAM, followed by AM. The main negatively correlated bacteria were Saccharibacteria (?=-0.4762, p=0.0187), unidentified__Saccharibacteria (?=-0.4739, p=0.0193) class, order and family level, and their relative abundances were significantly different: the highest in CK and PAM. Betaproteobacteria (?=-0.5669, p=0.0039) was extreme significantly negatively correlated with ADG, and its relative abundance was significantly different: the highest in CK. In conclusion, this study provides information on the association between rumen microbiome and ADG in feeding Allium Mongolicum regel extracts in sheep, which could reveal the integrative information of rumen function and lead to an improvement in ruminant growth.