E-MTAB-9586
RNA-seq of human liver cancer cell lines after lncRNA overexpression with CRISPRa
Biological replicates of Human liver cancer cell line Huh7 (n = 2-4) were treated with CRISPRa to overexpress (OE) LINC00326, LINC01419, LINC02119, novel lncRNAs MSTRG.12891, as well as and non-targeting (NT) control. Cells were harvested 48hrs after transfection. Total RNA was extracted and sequenced with a strand-specific paired end RNA-seq protocol.
Biological replicates of Human liver cancer cell line Huh7 (n = 2-4) were treated with CRISPRa to overexpress (OE) LINC00326, LINC01419, LINC02119, novel lncRNAs MSTRG.12891, as well as and non-targeting (NT) control. Cells were harvested 48hrs after transfection. Total RNA was extracted and sequenced with a strand-specific paired end RNA-seq protocol.