Vaccinia WTA
Transcriptomes of the Intermediate and Late Stages of vaccinia virus Replication
The infections were synchronized with a reversible inhibitor of DNA replication hydroxyurea and used a vaccinia virus mutant vRO-G8R that conditionally transcribes late genes to separate the intermediate and late classes of mRNAs and HeLa S3 cells. The mRNA was isolated and purified from different time points for sequenceing. the intermediate and late classes of mRNAs. The mRNA was isolated and purified from different time points for sequenceing.
The infections were synchronized with a reversible inhibitor of DNA replication hydroxyurea and used a vaccinia virus mutant vRO-G8R that conditionally transcribes late genes to separate the intermediate and late classes of mRNAs and HeLa S3 cells. The mRNA was isolated and purified from different time points for sequenceing.