GSE191278
Genome-wide identification of H3K27ac & H3K4me3-binding sequences upon LPS & CSF1 stimuli in mouse oocytes
We found that oocytes could arrange IgG production against adverse enviromental stimuli. We want to use chip-seq (cut-and-tag kit)  to identify how different stimuli (LPS, CSF1) impact the binding of H3K27ac & H3K4me3 to transcription-regulation sequences of IgG gene Overall design: Identify how two major modified histones, H3K27ac & H3K4me3, bind to the regulating sequences of IgG gene upon different stimuli (LPS, CSF1) through chip-seq in mouse oocytes