GSE136282
TBC1D3 suppression of the Histone Lysine N-methyltransferase G9a promotes the generation of neural progenitors and cortical expansion [RNA-seq]
we conducted RNA sequencing using human cerebral organoids treated with TAT peptides. Each group with one replicates. Overall design: Human cerebral organoids were treated with TAT peptides for two groups, that is, TAT-Scrambled (Short for TAT-Scr) with the sequence of YGRKKRRQRRR-FRVRYWFQGCHSEDPWR and TAT-465-481, a special peoptide designed for inhibition of TBC1D3, with the sequence of YGRKKRRQRRR-EGPWFRHYDFRQSCWVR. After 4 days incubation, human cerebral organoid tissues were extracted at cultured day 16 and bulk RNA from TAT-Scr group or TAT-465-481 group was extracted to be sequenced. TAT-Scr group has two replicates as TAT-S1 and TAT-S2. TAT-465-481 group has two replicates as TAT-E1 and TAT-E2.