GSE200977
A distinct immature low-density neutrophil population characterizes acute generalized pustular psoriasis [PBMC]
Generalized pustular psoriasis (GPP) is a severe disease featured by neutrophilic pustules and enhanced IL-36 inflammatory pathway in skin. Recently, a transcriptomic analysis of PBMCs and neutrophils in MPO-deficient GPP patients has been performed in stable disease state. However, transcriptomic profiling of PBMCs during acute flare of GPP is unclear. Here, we reported a predominant neutrophil signature in GPP PBMC and a marked increase in the CD66+CD16+ low-density neutrophils (LDNs) within the PBMC fraction of acute GPP patients. Transcriptomic and functional analysis of LDNs revealed a hypoinflammatory phenotype yet enhanced release of neutrophil granule proteases, implicating that LDNs might contribute to the IL-36-mediated inflammation in GPP patients. Overall design: Comparative gene expression profiling analysis of RNA-seq data for peripheral blood mononulear cells (PBMCs) from 8 generalized pustular psoriasis (GPP) patients, 8 psoriasis vulgaris (PV) patients and 8 healthy donors.