ena-STUDY-IHE Delft Institute for Water Education-23-06-2020-10:55:03:204-684
This NERC funded study collected data from viruses in groundwater from urban poor settlements in Arusha, Tanzania, Dodowa (Accra), Ghana, and Kampala, Uganda.
We mapped the dsDNA virus assemblage in groundwater below sub-Saharan urban poor settlements in Arusha (Tanzania), Dodowa (Ghana) and Kampala (Uganda). Our results indicated that about 80% of dsDNA virus sequences matched the order of Caudovirales, i.e. indigenous bacteriophages. 1.8% of the dsDNA virus sequences matched those of viral pathogens that infect humans and larger animals, which we defined as so-called Above-Ground Hosts (AGH). Within this group, the relative abundances of the genera Chordopoxvirinae, Alphaherpesvirinae and Betairidovirinae were highest. Culturable E. coli bacteria were found, even in deeper wells, indicating that all water was faecally contaminated. The community assemblages sampled in the cities were statistically significantly different from each other. Dissolved ions, population density and sanitary status had no significant influence, but pH and latitude did.  We concluded that the transport of dsDNA virus in groundwater was location specific, but was not determined by input concentrations (i.e. related to population density) or related to groundwater chemistry. We hypothesize that other parameters, like the presence of macro-pores, cause these variations in these shallow, highly populated, heavily polluted terrestrial groundwater systems. About 34% of Africa has similar hydrogeology, so this may affect many urban areas continent-wide.
We mapped the dsDNA virus assemblage in groundwater below sub-Saharan urban poor settlements in Arusha (Tanzania), Dodowa (Ghana) and Kampala (Uganda). Our results indicated that about 80% of dsDNA virus sequences matched the order of Caudovirales, i.e. indigenous bacteriophages. 1.8% of the dsDNA virus sequences matched those of viral pathogens that infect humans and larger animals, which we defined as so-called Above-Ground Hosts (AGH). Within this group, the relative abundances of the genera Chordopoxvirinae, Alphaherpesvirinae and Betairidovirinae were highest. Culturable E. coli bacteria were found, even in deeper wells, indicating that all water was faecally contaminated. The community assemblages sampled in the cities were statistically significantly different from each other. Dissolved ions, population density and sanitary status had no significant influence, but pH and latitude did.  We concluded that the transport of dsDNA virus in groundwater was location specific, but was not determined by input concentrations (i.e. related to population density) or related to groundwater chemistry. We hypothesize that other parameters, like the presence of macro-pores, cause these variations in these shallow, highly populated, heavily polluted terrestrial groundwater systems. About 34% of Africa has similar hydrogeology, so this may affect many urban areas continent-wide.