PRJNA842099
Machine Learning Optimized Cas12a Barcoding Enables Recovery of Single-Cell Lineages and Transcriptional Profiles
We present a compact, tunable, high-capacity Cas12a barcoding system, Dual Acting Inverted Site arraY (DAISY). A single DAISY barcode recovered up to ~700 lineages from one parental cell. This analysis revealed heritable single-cell gene expression and potential epigenetic modulation of memory gene transcription. Overall, Cas12a DAISY-barcoding is an efficient tool for investigating cell state dynamics.