CircleSeq in Human Fibroblasts
CircleSeq in Human Fibroblasts
Low abundance, circular RNAs composed of exonic sequence have been described arising from a small number of genes.  Originally thought to result from errors in splicing, circular RNA species possess no known function.  In an effort to delineate the universe of endogenous circular RNAs, we performed long-read, high-throughput sequencing of ribosome-depleted cDNA libraries prepared from total RNA with or without pre-digestion with the RNA exonuclease, RNAse R.