High_resolution_structure_of_hs21-sc-3431
High_resolution_structure_of_hs21
Here we attempt to describe the structure of Hs21 at high resolution using proximity ligation assays in combination with target enrichment in several conditions (yet to be determined). We will also generate additional epigenetic information that will help with the interpretation of the data.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Abstract*: Here we propose to use Capture Hi-C to generate a high-resolution structure of a whole human chromosome. As a starting point to establish Chromosome Capture Hi-C, we propose to capture the ensemble of chromosomal contacts made by human chromosome 21 in IMR90 cells (primary human fibroblasts, Encode tier 2) at unprecedented resolution. Human chromosome 21 has previously been used as a ‘model chromosome’ in pioneer transcriptome and chromatin immunoprecipitation (ChIP) studies 5. In addition, genome-wide Hi-C data for IMR90 fibroblasts are available. This data set, although affording a significantly lower resolution compared to what we aim to obtain using CCHi-C, will allow us to ‘benchmark’ our data using an independently generated chromosomal interaction data set.