GSE174650
Single-cell deconvolution of colon organoids exposed to carcinogens reveals novel insights into the early molecular events defining the role of smoking in colorectal cancer
Here, we used bulk RNA-seq data derived from healthy colon organoids (un)exposed to carcinogen. Through the use of external single cell RNA-seq data, we estimate changes in cell composition. We extend this analysis by controlling for cell composition and performing WGCNA to identify modules of co-expression differentially affected by carcinogen treatment in colon organoids. Overall design: Analysis of the effects of carcinogen treatment on gene expression in colon organoids