GSE135822
Hypolipidemic effects of Zexie Decoction on the lipid profile in hyperlipidemia rats by RNA-sequencing
RNA sequencing and transcriptome analysis were performed collaboratively, including analysis of differentially expressed genes (DEGs), GO functions and KEGG pathway analysis. The results showed that 1981 (1370 upregulated and 611 downregulated) DEGs were identified in the HFD group compared to those in the CON group. 474 DEGs (350 upregulated and 124 downregulated) were detected in the ZXD group compared to those in the HFD group. Furthermore, GO analysis revealed that DEGs were mainly involved in the following functions: developmental process, response to external stimulus, ion transport, alcohol binding and plasma membrane part. Pathway analysis suggested that these DEGs were significantly enriched in bile secretion, malaria, cell adhesion molecules, retinol metabolism, the sphingolipid signaling pathway, chemical carcinogenesis, and the T cell receptor signaling pathway. In conclusion, our study demonstrated that ZXD alleviated the lipid metabolism disorder caused by hyperlipidemia through multiple mechanisms, which provided vital scientific evidence for further pharmacological studies of ZXD. Overall design: In this study, we aimed to investigate the hyperlipidemic activity and the potential molecular mechanisms of ZXD in hyperlipidemia rats at the transcriptional level.