Ecoli_MG1655_100b
Paired-end sequencing of the genome of Escherichia coli K-12 strain MG1655 using the Illumina Genome Analyzer II
We generated a sequencing library with a median insert size of 214 bp following random fragmentation and gel fractionation of genomic DNA. 100 base sequence reads were generated from both ends of these templates using a single flowcell lane on the Illumina GA II. Sequence reads were aligned to the reference genome using ELAND.
E. coli K-12 holds a key position as a model organism in studies of molecular biology, biochemistry, genetics and biotechnology. A high-quality reference sequence of the genome of E. coli K-12 strain MG1655 has previously been generated using the Sanger dideoxy sequencing method (Reference sequence accession: NC_000913). We have re-sequenced the genome of the MG1655 strain using the Illumina Genome Analyzer II to provide a paired-end, 100 base sequence dataset for development of de novo sequence assembly algorithms or other downstream analysis tools.