GSE114338
IL-22+ T cells protect intestinal crypts from bacterial invasion and epithelial damage.
Three subsets of intestinal epithelial cells (IECs) (P9, Surface; P10, Large Crypt; P11, Small Crypt) were isolated from Naïve and Day 9 C.r.-infected Cntrl (Cre-) and CD4 cre+. Il22 floxed mice (CD4 cKO) Overall design: IECs from middle/distal colon regions (pooled from 2-4 mice per sample) were isolated with DTT, followed by EDTA, and then sorted into three populations (All were CD45-LD-EpCAM1+): CEACAM1Hi Surface, CEACAM1Int/lo SSCHi Large Crypt, and CEACAM1- FSClo SSClo Small Crypt cells