ena-STUDY-CARDIFF UNIVERSITY-18-02-2015-09:35:51:024-37
Whole genome sequencing project to investigate mobile elements harbouring blaNDM-1 and other resistance determinants from Acinetobacter baumannii isolated in Tamil Nadu, India
Extensively drug resistant Acinetobacter baumannii isolated from clinical isolates collected in Tamil Nadu in 2005 were found to produce NDM-1. 3 representative isolates were sequenced by illumina and assembled de novo using velvet. Functional annotation was provided using RAST, and then refined following comparison with homologous reference sequences. Plasmid pNDM-32 from strain CHI-32 was assembled completely with PCR and sequencing of amplicons to confirm the final assembly.
Extensively drug resistant Acinetobacter baumannii isolated from clinical isolates collected in Tamil Nadu in 2005 were found to produce NDM-1. 3 representative isolates were sequenced by illumina and assembled de novo using velvet. Functional annotation was provided using RAST, and then refined following comparison with homologous reference sequences. Plasmid pNDM-32 from strain CHI-32 was assembled completely with PCR and sequencing of amplicons to confirm the final assembly.