GSE208380
RNA-sequencing data of Tip60 deleted and control mouse embryonic fibrobasts
In this study we examined the effects of loss of the MYST histone acetyltransferase TIP60 (KAT5) in mouse embryonic fibroblasts (MEFs), human embryonic kidney cells 293 (HEK293), and human osteosarcoma cells (U2OS) on cell proliferation, BrdU incorporation, cell cycle progression, apoptotic and other forms of cell death, DNA damage, histone acetylation at specific lysine residues and RNA expression levels. This dataset relates to MEFs. To assess the effects of loss of TIP60 on RNA levels, RNA-seq was performed on MEFs, where the TIP60 gene was deleted using Cre/loxP technology. Nuclear translocation was induced with 4-OH-tamoxifen treatment for 3 and 5 days to induce TIP60 gene deletion in the samples also containing the loxP sited in the Tip60 locus. Overall design: RNA-sequencing data was generated for 14 mouse embryonic fibroblast samples. These samples are either control or Tip60 knock-out (KO), that have been treated for either 3 or 5 days to induce Tip60 KO. Differential analyses were undertaken to identify differential genes between KO and control groups at both 3 and 5 days.