PRJNA662561
Metallothioneins promoter DNA methylation
Mechanisms underlying hepatocellular carcinoma (HCC) development are largely unknown and interest was recently dedicated to a potential role of trace elements and to metallothioneins (MTs), proteins regulating metal ions homeostasis and detoxification. In the present study, we investigated the role of promoter DNA methylation in the transcriptional repression of two metallothioneins, i.e. MT1G and MT1H, in human HCC tissues. DNA methylation was assessed by next generation sequencing (NGS) performing bisulfite amplicon sequencing of a region overlapping MT1G and MT1H promoter.