GSE171152
The uncharacterized gene Rnf149 is a regulator of cardiopharyngeal development
To assay Rnf149 function during early cardiopharyngeal specification, bulk RNAseq was performed to profile the FACS-purified cardiopharyngeal lineage cells isolated from20 hpf Ciona larvae with CRISPR/Cas9-mediated Rnf149 mutagenesis. Overall design: Examination of CRISPR/Cas9-mediated Rnf149 mutagenesis in the cardiopharyngeal lineage at 20 hpf.