PRJNA731598
Short- and long-read RNA-seq of mouse neural stem cells across five developmental stages
Mouse neural stem cells (NSC) across five developmental stages (E15.5, E17.5, P1.5, P8 and Adult) were collected via fluorescence-activated cell sorting with CD133 and Blbp-GFP as NSC markers. The harvested NSCs were subjected to SMART-seq2 protocol and RNA-seq with both Illumina short-read and Oxford Nanopore long-read sequencing strategies.