GSE185143
Transcriptome profiling from Wild Type and Lss mutant (heterozygous and homozygous) lens at E14.5 reveals the potential pathways involved in cataract formation
Congenital cataract is one of the leading causes of blindness in children worldwide. About one third of congenital cataracts are caused by genetic defects. Previous studies have identified LSS as a causal gene for congenital cataracts. However, its roles in lens development remains largely unknown. Here, we performed RNA-seq on the lens of WT and Lss mutant (heterozygous and homozygous )at E14.5, identified that cholesterol synthesis signaling pathways were significantly downregulated. Overall, our study points out that LSS functions as a critical determinant of lens development, which will contribute to better understand LSS defects in cataractogenesis and develop therapies for cataract. Overall design: RNA-seq profiles of lens from WT and Lss mutant (heterozygous and homozygous) at E14.5 mouse