ena-STUDY-COST Action-22-01-2018-14:14:12:353-456
Ring Test for fecal DNA extraction protocols in pigs
The PiGutNet COST action organised a ring test to evaluate different DNA extraction protocols for porcine gut microbiota. A set of pooled samples were distributed to several laboratories along Europe and each group performed a set of extraction protocols. Extracted DNA was subjected to 16S sequencing (V3-V4 region) in an Illumina MiSeq instrument.
The PiGutNet COST action organised a ring test to evaluate different DNA extraction protocols for porcine gut microbiota. A set of pooled samples were distributed to several laboratories along Europe and each group performed a set of extraction protocols. Extracted DNA was subjected to 16S sequencing (V3-V4 region) in an Illumina MiSeq instrument.