PRJNA796457
16S ribosomal RNA gene sequencing of gastrointestinal tract contents derived from specific pathogen free wild-type C57Bl/6J, C1qc-/-, and C3-/- mice
To analyze microbiota community composition, total genomic DNA from the indicated contents derived from specific pathogen free (SPF) wild-type (WT) C57Bl/6J, C1qc-/-, and C3-/- mice, was extracted using phenol:chloroform:isoamyl alcohol solution following extensive bacterial interruption and lysis with Silica glass beads. The V4 region of 16S rRNA gene were amplified with the published primer pair of 515F and 806R, followed by sequencing on an Ion S5TM XL platform (400-bp single read) or a NovaSeq 6000 platform (250-bp paired end reads).