ena-STUDY-FUDAN UNIVERSITY-07-04-2016-03:41:16:053-410
MTB isolates collected from 12 patients during antibiotics treatment
We collected serial sputum samples from TB patients during antibiotics treatment. Three sputum samples were collected at each time points of week 0, 2, 4, 6, 8, 16 and 24. All the isolates were performed both L-J and MGIT culture. All the colonies on L-J plate were scraped and extracted DNA for whole genome sequencing. If the sputum sample was L-J culture negative but MGIT culture positive, the MGIT culture was used for whole genome sequencing. The average sequencing depth was ~1000X.
We collected serial sputum samples from TB patients during antibiotics treatment. Three sputum samples were collected at each time points of week 0, 2, 4, 6, 8, 16 and 24. All the isolates were performed both L-J and MGIT culture. All the colonies on L-J plate were scraped and extracted DNA for whole genome sequencing. If the sputum sample was L-J culture negative but MGIT culture positive, the MGIT culture was used for whole genome sequencing. The average sequencing depth was ~1000X.