PRJNA685320
Abelmoschus esculentus L SMRT Raw sequence reads
Moreover, a combination of third- (PacBio) and second-generation (Illumina) sequencing technologies was applied to sequence full-length transcriptomes of okra. In order to get as many transcripts as possible, total RNA was extracted from three types of okra tissue (roots, stems and leaves).The high-quality RNA of different tissues was mixed equally and then used for library construction. Libraries were sequenced on the PacBio Sequel platform. Standard Iso-Seq classification and clustering protocol was used to obtain the full transcript sequence.