GSE127546
Smarcad1 coordinates innate immunity-linked gene expression in the intestinal epithelium [16S Amplicon-seq]
Smarcad1 is one of the most conserved chromatin remodelling factors, from yeast to human, with suggested functions in gene silencing, heterochromatin maintenance and genome stability. However, its role in tissue function is poorly understood. As this factor is highly expressed in the stem- and proliferative zone in the intestinal epithelium, we explored the role of this factor in this tissue. Specific deletion of Smarcad1 in the intestinal epithelium led to colitis resistance in the dextran sodium sulphate colitis model. We identified candidate members of the gut microbiome that elicit an epithelial Smarcad1-dependent colitis response. Overall design: Compared genotypes: wild type mice and Vil-Cre mediated KO of Smarcad1 in the small intestinal epithelium. C57BL/6 background. Enriched microbiota by bedding transfer from another SPF-facility. 16S Amplicon-Seq performed on donor microbiota (triplicates) and WT/KO microbiota before and after enrichment (10 replicates per cohort), 43 samples total.