PRJNA485663
Role of USP47 in CD8+ T cell function
Fresh splenic CD8+ T cells were isolated from 6-week-old Usp47+/+Cd4-Cre and Usp47fl/flCd4-Cre mice and stimulated with anti-CD3 and anti-CD28 for 24 hours. Activated CD8+ T cells were used for total RNA isolation with TRIzol and subjected to RNA-sequencing.