GSE201199
Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes
To identify differently expressed genes between ferret and mouse astrocytes, we performed RNA-seq studies on primary ferret and mouse astrocytes cultured in serum free medium. We isolated primary astrocytes from mixed glial cultures which are prepared from the cortices of ferrets and mice at postnatal day 1. Each biological replicate was generated from 2 individuals of the same species. We detected substantial changes in the expression of genes associated with cell proliferation and migration, and identified ferret astrocyte-specific genes. Overall design: We generated mRNA profiles of primary astrocytes which were prepared from ferret and mouse neonatal cortices using Illumina HiSeq 2000 platform.