BSF_gut
Abundance and phylogenetic diversity of the intestinal bacterial community                 in the food waste reducing larvae of Hermetia illucens
As it is known that food waste can be reduced by larval Hermetia                 illucens (Black Soldier Fly, BSF), the scientific and commercial value of larval BSF                 is increased recently. We focused one of the reasons that ability of catabolic                 degradation might be caused by intestinal microorganisms, here we analyzed bacterial                 community of BSF larval gut. We extracted their intestinal metagenomic DNAs from                 each larva provided with three different nutrients. We performed polymerase chain                 reactions (PCR) by using bacterial 16S rRNA primers and pyrosequencing for the                 analysis of intestinal microbial community. We got the 16S rRNA sequence data set of                 9737, 9723, and 5985 sequences from the PCR products of three different fed samples,                 food waste, cooked rice, and calf forage, respectively. On the basis of the BLAST                 search of EzTaxon program, the bacterial community of the food waste fed gut was                 revealed that the phyrum Bacteroidetes had dominancy of 67.6%, Proteobacteria of                 18.7%, and Firmicutes of 9.6%. The phylogenic groups from cooked rice fed gut were                 two dominant phyrums of Firmicutes (42.64%) and Proteobacteria (54.47%). The                 phylogenic groups from calf forage fed gut were four similar portions of Firmicutes                 (23.46%), Actinobacteria (24.58%), Bacteroidetes (25.01%), and Proteobacteria                 (31.08%). Despite the bacterial community structures were depended on feeding                 nutrient sources, the identified bacterial strains were revealed unique species of                 BSF gut unlike other intestinal microflora. Here, we analyzed and discussed the                 bacterial community structures, possible unique bacterial flora of BSF larval gut,                 and organic compounds degrading enzymes producing bacterial                 strains.