GSE159032
Cellular drivers of injury response and regeneration in the adult zebrafish heart
We dissected cell type diversity in the regenerating zebrafish heart by single cell transcriptomics and discovered a high complexity of cell types specifically among the non-muscle cells. Based on spatiotemporal information, we systematically identified potential cellular regulators of cardiomyocyte regeneration, including several fibroblast-like cell types that express pro-regenerative factors. We used high-throughput lineage tracing12 to determine the origin of cell types in the regenerative niche. We showed that the endocardium, like the epicardium, gives rise to several distinct fibroblast cell types, and found that this process is regulated by Wnt signalling. Our results reveal an unexpected functional specialization of fibroblast cell types during the regenerative process. Overall design: Single cell RNA seq of uninjured and injured / wnt-inhibited and non-inhibited zebrafish hearts. CRISPR-based lineage tracing in a subset of these hearts. RNA velocity analysis in a subset of these hearts.