GSE168901
Single-cell Transcriptomic Analysis of the Mouse Crista Ampullaris
scRNA-seq on E18, P3 and P7 mouse cirsta ampullaris Overall design: Cristae, including the ampullae, were dissected from E16 (n=6), E18 mice (n = 5), P3 mice (n = 8) and P7 mice (n= 12) in ice cold Hank's buffered salt solution. Pooled cristae were then dissociated by treatments with collagenase and papain. Libraries for scRNA-seq were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics; Pleasanton, CA) per the manufacturer's instructions. Reads were aligned to mm10 and filtered using the Cell Ranger pipeline.