PRJNA563975
mRNA sequencing (RNA-sequencing ) of human ß-cell model EndoC-ßH1
The aim of the project is the detect changes in global gene expression of protein coding genes after the knockdown of SLC30A8 gene in human beta cell model - EndoC-ßH1. For RNA sequencing post 96h siScramble (n=8) or siSLC30A8 (n=8) transfected EndoC-ßH1 cells were used and RNA sequencing was performed using Illumina TruSeq-mRNA library on NextSeq 500 system (Illumina) with paired-end reads (2 × 75 base pairs).