GSE174587
Puf6 primes nuclear export of 60S pre-ribosomes at low temperature
Puf6 contributes to biogenesis of the large subunit in yeast. UV-crosslinking (CRAC) was used to determine binding sites of Puf6 on RNA. Overall design: Duplicate data-sets of cDNA reads of RNAs UV-crosslinked to PUF6-HTP in vivo using the CRAC protocol. Cells were in mid-log phase when irradiated, immediately collected by filtration and frozen