ena-STUDY-IPK-Gatersleben-31-07-2020-12:45:39:671-2134
Transcriptional landscape of floral meristem fates in barley
Laser capture microdissection (LCM)-based RNA-seq of barley spike tissues. Inflorescence meristems (IM), leaf ridges (LR) and spikelet ridges (SR), central (CS) and lateral spikelets (CS) were isolated at six initial stages of barley spike development ranging from double ridge (DR) to awn primordium (AP) stage. Control samples of vegetative meristems (vegetative apex (VA), root apical meristem (RAM), leaf blade (LB)) and different growth conditions (shading/stress) are also included in the data set for comparison to spike tissues. Four biological replicates were generated from each tissue-type (exception of triplicate samples for vegetative meristems) resulting in 209 RNA-seq samples.
Laser capture microdissection (LCM)-based RNA-seq of barley spike tissues. Inflorescence meristems (IM), leaf ridges (LR) and spikelet ridges (SR), central (CS) and lateral spikelets (CS) were isolated at six initial stages of barley spike development ranging from double ridge (DR) to awn primordium (AP) stage. Control samples of vegetative meristems (vegetative apex (VA), root apical meristem (RAM), leaf blade (LB)) and different growth conditions (shading/stress) are also included in the data set for comparison to spike tissues. Four biological replicates were generated from each tissue-type (exception of triplicate samples for vegetative meristems) resulting in 209 RNA-seq samples.