PRJNA521077
RNA-seq and ribosome footprint profiling of human and mouse platelets with sepsis
There is increasing recognition that platelets have a functional role in the pathophysiology of sepsis though have not been precisely defined. Whether sepsis alters the human platelet transcriptome and translational landscape has never been established. We utilized parallel techniques of RNA-sequencing and ribosome footprint profiling to interrogate the platelet transcriptome and translatome in septic patients and healthy donors. We identified 1,806 significantly differentially expressed (FDR<0.05) transcripts in platelets from septic patients. Translational events in platelets during sepsis were also upregulated. To explore relevance of murine model of sepsis, cecal ligation and puncture (CLP) we compared sepsis-induced changes in platelet gene expression between septic patients and CLP-treated mice. Platelet transcriptional (rho=0.42, p=3.2x10-285) and translational (0.65, p=1.09x10-56) changes were significantly correlated between septic patients and mice. We focused on ITGA2B, tracking and validating the expression, regulation, and functional impact of changes in ITGA2B during sepsis. ITGA2B expression was significantly upregulated in human and murine platelets during sepsis. Increased ITGA2B was identified in bone marrow megakaryocytes within 24 hours of sepsis onset, with subsequent trafficking to platelets at 72 hours. Transcriptional changes in ITGA2B were accompanied by de novo protein synthesis of ?IIb and integrin ?IIb?3 activation. Increased ?IIb was associated with mortality in humans and mice. These findings provide previously unrecognized evidence that human and murine sepsis similarly alters the platelet transcriptional and translational landscape. Moreover, ITGA2B is upregulated and functional in sepsis due to trafficking from megakaryocytes and de novo synthesis in platelets, and is associated with increased mortality.