3dbdd4c9-155a-4678-852e-eaee5a0f2689
5'RACE of mouse Grxcr1 showing a novel isoform
5' RACE PCR (5' rapid amplification of 5' cDNA ends) was performed to analyse the mRNA structure and expression of Grxcr1 by generating cDNA from the mouse Grxcr1 gene. The resulting 5' RACE products were cloned into a vector for sequencing and subsequent manipulation. We used a 5'/3' RACE Kit, 2nd generation (Roche) and mRNA from wild-type mouse ear and brain at P0 and P6 (n=2). We observed two sequences, one including all four known exons and one skipping exon 2, which is a novel transcript. This is the sequence we are reporting here.
5' RACE PCR (5' rapid amplification of 5' cDNA ends) was performed to analyse the mRNA structure and expression of Grxcr1 by generating cDNA from the mouse Grxcr1 gene. The resulting 5' RACE products were cloned into a vector for sequencing and subsequent manipulation. We used a 5'/3' RACE Kit, 2nd generation (Roche) and mRNA from wild-type mouse ear and brain at P0 and P6 (n=2). We observed two sequences, one including all four known exons and one skipping exon 2, which is a novel transcript. This is the sequence we are reporting here.