GSE168896
Biphasic effects of pleiotropic cytokine IL-27 during Staphylococcus aureus implant-associated osteomyelitis
We aimed to elucidate the mechanism of IL-27/ IL-27 receptor signaling effects on S. aureus osteomyelitis via gene expression studies. Overall design: C57BL/6 mice were intramuscularly injected with recombinant adeno-associated virus expressing murine IL-27 (rAAV-IL-27) or recombinant adeno-associatd virus expressing GFP (rAAV-GFP, control) and then challenged with a S. aureus contaminated trans-tibial implant. Subsequently, infected tibia were collected on days 1, 3, 7 and 14 post-septic surgery for RNA sequencing.