GSE135960
CDK12 inhibition in Hep3B, Huh7 and SNU449 cells
Purpose: to analyze gene expression in THZ531 treated liver cancer cells. Methods: Hep3B and Huh7 cells are treated with THZ531 for 24 hours. SNU449 cells are treated with THZ531 for 48 hours. For RNA sequencing, the library was prepared using TruSeq RNA sample prep kit according to the manufacturer's protocol (Illumina). Gene set enrichment analysis was performed using gene set enrichment analysis software. Overall design: RNA seq data of Hep3B-control, Hep3B-250nM, Hep3B-500nM, Huh7-control, Huh7-250nM, Huh7-500nM, SNU449-control, SNU449-250nM, SNU449-500nM,  to check the effects of THZ531 on globle gene expression.