GSE124323
Epithelial Vegfa specifies a distinct endothelial population in the mouse lung [scRNA-seq]
The lung microvasculature is essential for gas exchange and commonly considered homogeneous.  We show that Vascular endothelial growth factor A (Vegfa) from the epithelium specifies a distinct endothelial cell (EC) population in the postnatal mouse lung.  Vegfa is predominantly expressed by alveolar type 1 (AT1) cells and locally required to specify a subset of ECs.  Single cell RNA-seq identified 15~20% lung ECs as transcriptionally distinct and marked by Carbonic anhydrase 4 (Car4), which are specifically lost upon epithelial Vegfa deletion.  Car4 ECs, unlike bulk ECs, have extensive cellular projections and are separated from AT1 cells by a limited basement membrane without any intervening pericytes.  Without Car4 ECs, the alveolar space is aberrantly enlarged despite the normal appearance of myofibroblasts.  Lung Car4 ECs and retina tip ECs have common and distinct transcriptional profiles.  These findings support a signaling role of AT1 cells and shed light on alveologenesis. Overall design: FACS-purified lung endothelial cells were processed through the Chromium Single Cell Gene Expression Solution Platform (10X Genomics)