ena-STUDY-BioISI - Faculty of Sciences, University of Lisbon-16-07-2021-18:39:12:233-262
Transcriptome analysis of 16HBE cell line models harbouring homozygous Cystic Fibrosis causing mutations
The aim of this study was to generate a comparative overview of the impact of different CF-causing mutations on the transcriptome of human bronchial epithelial cells (16HBE14o-). For this purpose, we performed paired-end Illumina mRNA-Seq from total RNA samples isolated from WT and CRISPR gene edited cell lines carrying different homozygous CFTR mutations (F508del, N1303K, G551D and I1234V), using three independent biological replicates.
The aim of this study was to generate a comparative overview of the impact of different CF-causing mutations on the transcriptome of human bronchial epithelial cells (16HBE14o-). For this purpose, we performed paired-end Illumina mRNA-Seq from total RNA samples isolated from WT and CRISPR gene edited cell lines carrying different homozygous CFTR mutations (F508del, N1303K, G551D and I1234V), using three independent biological replicates.