Transcriptome analysis of Aegilops variabilis
Aegilops peregrina strain:No. 1 Transcriptome or Gene expression
Aegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). Using RNA-Seq technology, a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 was generated from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with average read-depth coverage of 33.25×. In BLAST searches of our database against public databases, 66.46%% (78,467) of the unigenes were annotated with gene descriptions, conserved protein domains, or gene ontology terms. Functional categorization further revealed 7,408 individual unigenes and three pathways related to plant stress resistance.