periclinal chimera study
Using a perclinal chimera to determine layer-specific gene             expression
The aim of this study was to identify the layer specific transcriptome in             a mixture of tomato cell types. A tomato L1 periclinal chimera was generated that has an             epidermal L1 layer from Solanum pennellii and internal L2 and L3 layers from Solanum             lycopersicum. SOLiD RNA-seq data was generated from the chimera and the parental lines             using different tissue samples (leaf, leaf under water deficit conditions and mature             green fruit). Mapping the transcriptomic reads of the parental lines to the S.             lycopersicum genome sequence enabled the identification of polymorphisms within each             species. Reads from the chimera were assessed as coming from one or other of the             parental species based upon the single nucleotide polymorphisms detected and the species             specific gene expression was defined. By comparing the S. pennellii and S. lycopersicum             specific expression in the chimera with the related expression in the parental lines,             genes with layer-specific expression were identified.
The aim of this study was to identify the layer specific transcriptome in             a mixture of tomato cell types. A tomato L1 periclinal chimera was generated that has an             epidermal L1 layer from Solanum pennellii and internal L2 and L3 layers from Solanum             lycopersicum. SOLiD RNA-seq data was generated from the chimera and the parental lines             using different tissue samples (leaf, leaf under water deficit conditions and mature             green fruit). Mapping the transcriptomic reads of the parental lines to the S.             lycopersicum genome sequence enabled the identification of polymorphisms within each             species. Reads from the chimera were assessed as coming from one or other of the             parental species based upon the single nucleotide polymorphisms detected and the species             specific gene expression was defined. By comparing the S. pennellii and S. lycopersicum             specific expression in the chimera with the related expression in the parental lines,             genes with layer-specific expression were identified.