GSE112309
Gene expresssion profiles in PPARalpha-Serine280 phosphorylation mutants
PPARalpha regulates cardiac fatty acid metabolism. GSK-3alpha phosphorylates PPARalpha at Serine 280, which increases PPARalpha transcriptional activity in cardiomyocytes. Overall design: We transduced H9C2 cells with pDC316-YFP-PPARalpha-wild-type (WT), pDC316-YFP-PPARalpha-S280A phospho-resistant mutant, pDC316-YFP-PPARalpha-S280D phospho-mimicking mutant or pDC316-YFP alone for 2 days. Each n=2.