GSE186170
Characterization of Mfsd2a-deficient lung AT2 cells.
RNA-seq was performed to understand transcriptome changes with Mfsd2a deficiency in lung AT2 cells. Gene expression analysis was performed using DESeq2. Overall design: Mfsd2a floxed mice (2afl/fl) were generated as described previously (Wong et. al., JBC, 2016). Tamoxifen-inducible AT2-specific knockout mice (AT2aKO) were generated by crossing 2afl/fl with Sftpc-Cre/ERT2 driver [B6.129S-Sftpctm1(cre/ERT2)Blh/J. Total RNA was extracted from AT2 cells isolated from 2 weeks post-tamoxifen induced 2afl/fl or AT2aKO mice for RNA-sequencing. 4 mice per genotype was used.