PRJNA377342
Population genomic analysis of Brook Trout Salvelinus fontinalis in Pennsylvania’s Appalachian region
A double digest restriction site-associated DNA sequencing (ddRAD-seq) protocol (Protocol S1; Peterson et al. 2012) with EcoRI and SphI was performed on samples of S. fontinalis on approximately one lane of Illumina HiSeq. Sixteen barcodes combined with each of four indices were used for a total collection of 64 individuals.