ena-STUDY-ED-13-10-2017-10:33:01:802-117
Little evidence for introgression between Geum rivale and Geum urbanum
We used ddRADseq in a comparative genomic context to test for introgression (gene flow between species) between two plant species that differ in mating system:  Geum rivale (outcrosser) and G. urbanum (inbreeder).  We obtained samples from up to 5 reference populations (3 UK, 1 Iceland, 1 Sweden) for G. rivale and 12 reference populations for G, urbanum (10 UK, 1 France, 1 Portugal).  The reference populations were selected from areas where introgression was unlikely for a long period of time (>5000 years).  We used the reference populations to look for signals of gene flow between the species in a broad area of sympatry, comprising 39 populations (Berwickshire, Scotland).  A variety of analyses (coalescent analyses, f3 test, comparison of genetic divergence (i.e., d_xy), cluster analysis and calculation of hybrid indices based on species-specific SNPs) revealed little evidence for introgression, despite common hybridization and back-crossing to G. rivale.Double digest was performed using enzymes EcoRI and SphI
We used ddRADseq in a comparative genomic context to test for introgression (gene flow between species) between two plant species that differ in mating system:  Geum rivale (outcrosser) and G. urbanum (inbreeder).  We obtained samples from up to 5 reference populations (3 UK, 1 Iceland, 1 Sweden) for G. rivale and 12 reference populations for G, urbanum (10 UK, 1 France, 1 Portugal).  The reference populations were selected from areas where introgression was unlikely for a long period of time (>5000 years).  We used the reference populations to look for signals of gene flow between the species in a broad area of sympatry, comprising 39 populations (Berwickshire, Scotland).  A variety of analyses (coalescent analyses, f3 test, comparison of genetic divergence (i.e., d_xy), cluster analysis and calculation of hybrid indices based on species-specific SNPs) revealed little evidence for introgression, despite common hybridization and back-crossing to G. rivale.Double digest was performed using enzymes EcoRI and SphI