GSE193997
ATF3 Inhibits Cardiac Ferroptosis  by Directly Binding FANCD2 in Response to Myocardial Ischemia/Reperfusion Injury [RNA-Seq 1]
The goals of this study are to compare  mice heart tissues' transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis, and find the potential molecule implicating in myocardial ischemia/reperfusion injury. Overall design: mRNA profiles of wild-type (WT) mouse heart tissues after ischemia for 45 min followed by different reperfusion durations(I/R 0.5h, 1.0g, 1.5h, 3.0h, 6.0h, 12h) by deep sequencing, in triplicate.