e4bcd631-ed61-40e4-b70c-deba69da443a
RNA-seq of roots from two Coffea arabica cultivars Catuaí Vermelho (IAC 144) and Obatã (IAC 1669-20) submitted to hexanoic acid application.
Five-month-old plants of Coffea arabica cv. Catuaí Vermelho (IAC 144) and Obatã (IAC 1669-20) were transferred to pots containing 3 L of aerated nutrient solution (ANS). The hexanoic acid experiment was carried out as described in Silva et al. (2020), under controlled temperature (23±2ºC) and light/dark cycle (12h/12h, photosynthetically active photon flux density of ~400 µmol.m-2.s-1). The following treatments were assessed: (a) ANS (control); (b) ANS + hexanoic acid (Merck, final concentration 0.55 mM) for 48 h. Plants were grown in 3 to 6 plastic pots in which three pots received each treatment. Total RNA was extracted from roots pools using the RNeasy Plant kit (Qiagen, Hilden, North Rhine-Westphalia, Germany).
Five-month-old plants of Coffea arabica cv. Catuaí Vermelho (IAC 144) and Obatã (IAC 1669-20) were transferred to pots containing 3 L of aerated nutrient solution (ANS). The hexanoic acid experiment was carried out as described in Silva et al. (2020), under controlled temperature (23±2ºC) and light/dark cycle (12h/12h, photosynthetically active photon flux density of ~400 µmol.m-2.s-1). The following treatments were assessed: (a) ANS (control); (b) ANS + hexanoic acid (Merck, final concentration 0.55 mM) for 48 h. Plants were grown in 3 to 6 plastic pots in which three pots received each treatment. Total RNA was extracted from roots pools using the RNeasy Plant kit (Qiagen, Hilden, North Rhine-Westphalia, Germany).