CRISPR_mediated_mutation_of_GFP_mouse_embryos-sc-3100
CRISPR_mediated_mutation_of_GFP_mouse_embryos
CRISPR/Cas9 inducing mutations in GFP positive mouse embryos
Direct injection of different concentrations of gRNA and Cas9 in one cell mouse embryos resulting in mutations. In order to identify the degree of mosaics as well as the kind of mutation, mysequ runs are performed at blastocyst stage.