Pilot_Sequencing_of_Multiplexed_gRNA_Library-sc-5961
Pilot_Sequencing_of_Multiplexed_gRNA_Library
We have developed a CRISPR/Cas9 platform for multiplexed analysis of gene essentiality in human cells. This is a pilot experiment aimed to verify that our current protocol can be scaled up effectively, for future upscaling.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
We have developed a minimised guide RNA library that allows a 3-fold reduction in size and cost of genome-wide CRISPR-Cas9 screen, within the framework of a larger project aimed to understand to which extent gene essentiality varies in healthy humans. This new minimised libraries required the development of a new protocol for the sequencing of the amplicon from genomic DNA. The aim of this pilot is to verify that the new protocol performs well and is scalable.