E-MTAB-7136
RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm40600 against vector control
We found that some novel genes such as Gm40600 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm40600 overexpression on plasmablast/plasma cells. Gm40600 cDNA was cloned into lentiviral vector LV201 to generate Gm40600 protein. Gm40600-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection. To identify the effect of Gm40600 overexpression on gene expression, we determined mRNA profiles in Gm40600-overexpressed and vector-transduced SP 2/0 cells by RNA-sequencing. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.
We found that some novel genes such as Gm40600 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm40600 overexpression on plasmablast/plasma cells. Gm40600 cDNA was cloned into lentiviral vector LV201 to generate Gm40600 protein. Gm40600-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection. To identify the effect of Gm40600 overexpression on gene expression, we determined mRNA profiles in Gm40600-overexpressed and vector-transduced SP 2/0 cells by RNA-sequencing. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.