PRJNA865461
heart Raw sequence reads
After obtaining the raw sequencing data (Raw Data), it is first necessary to filter the data, remove the adapter sequence, and process the low-quality reads.The sequencing quality was then evaluated to obtain high-quality data (Clean Data). And compare Clean Data with reference genome to get BAMdocument.For the detected mRNA, further calculate the gene expression and analyze the differentially expressed genes between samples, and perform GeneOntology (GO) analysis, KEGG biological pathway enrichment analysis. For the detected lncRNAs, the lncRNA expression levels and scores were calculated accordingly.Differentially expressed lncRNAs between samples were analyzed and specific annotations were given. Finally, the corresponding advanced analysis is formulated according to the needs of the customer.