PRJNA543389
Arabidopsis thaliana WGBS data for methhap performance test
Hybrids between Arabidopsis thaliana Cape Verde Islands (Cvi-0) and Landsberg erecta (Ler-0) were generated by hand pollination. Seeds of Arabidopsis thaliana (Ler-0,) were sterilized and sown onto MS medium plates containing 1% (wt/vol) sucrose and 0.2% (wt/vol) phytagel. After 4 d at 4 °C, the plates were transferred to a growth chamber at 22 °C under white light (100 mmol m22 s21) conditions (16 h light/8 h dark). The 10-d-old seedlings were used for MethylC-seq assays, around 200 mg seedlings were pooled in each sample for genomic DNA extractions as described previously(Allen et al., 2006). Libraries for high-throughput bisulfite sequencing were generated as described previously (Wang et al., 2013).