GSE126142
Genome-wide mapping of CASZ1b binding sites and investigate its effect on chromatin status in SMS-CTR cells [CTRtetCASZ1b]
In SMS-CTR cells, we identified genomewide binding sites of CASZ1b. The overexpression of CASZ1b in SMS-CTR cells led to a regional epigenetic modification. Overall design: In the CASZ1b stably transfected SMS-CTR cells (CTRtetCASZ1b), CASZ1b expression is Doxcycline (Dox) inducible . The CTRtetCASZ1b cells were treated with or without Dox for 48 hr, and ChIP seq was performed using anti-CASZ1 antibody and antibodies of histone markers and RNA Pol II.