DRP004473
Effects of local gut tumor on whole-organismal gene expressions in zebrafish
How tumors affects whole-organismal physiology remains largely unknown. To address this, we established the novel gut tumor model in zebrafish, Danio rerio. This model develops tumor at an early stage of juvenile development, when zebrafish larvae are small (<4mm), enabling us to perform whole-organismal RNA-seq experiments. Control or tumor-bearing zebrafish were dissected into the three parts under microscope: the liver, the gut/gut tumor, and others. Tissues from >10 individuals were pooled and RNA-extracted. Analyses on these RNA-seq samples identified a set of host genes affected by the gut tumor, contributing to discovering novel tumor-organ interactions and their mediators in zebrafish.