E-MTAB-1946
arabidopsis_thaliana_floral_meristem_atlas
Transcriptomic changes during early developmental stages of reproductive meristems in Arabidopsis thaliana. Through laser microdissection coupled to RNA sequencing, we provide precise transcriptomic analysis of the inflorescence meristem, floral meristem and of the flower at the beginning of organ differentiation. Transcript abundance quantification and differential expression (DE) analyses were performed using the Bowtie/Tophat/Cufflinks pipeline (BTC) (Trapnell et al., 2012) with the RefSeq Arabidopsis annotation. The BTC pipeline identified 46 genes as differentially expressed between IM and FM, 171 genes differentially expressed between FM and ST3 and 178 genes differentially expressed between IM and ST3. The obtained data turned out to be solid since they are highly consistent with established knowledge but they also allowed the identification of new high profile candidate genes as well as new insights into potential regulatory networks underlying the formation of these reproductive structures. We provide a web interface for easy interrogation of these unique datasets.
Transcriptomic changes during early developmental stages of reproductive meristems in Arabidopsis thaliana. Through laser microdissection coupled to RNA sequencing, we provide precise transcriptomic analysis of the inflorescence meristem, floral meristem and of the flower at the beginning of organ differentiation. Transcript abundance quantification and differential expression (DE) analyses were performed using the Bowtie/Tophat/Cufflinks pipeline (BTC) (Trapnell et al., 2012) with the RefSeq Arabidopsis annotation. The BTC pipeline identified 46 genes as differentially expressed between IM and FM, 171 genes differentially expressed between FM and ST3 and 178 genes differentially expressed between IM and ST3. The obtained data turned out to be solid since they are highly consistent with established knowledge but they also allowed the identification of new high profile candidate genes as well as new insights into potential regulatory networks underlying the formation of these reproductive structures. We provide a web interface for easy interrogation of these unique datasets.