GSE172020
RNA-sequence on kidneys of wild type C57BL/6J and ARLmon/Y mice that underwent orchidectomy and testosterone (or vehicle) replacement for 4 days
We generated a mouse model (ARLmon/Y) with disrupted dimerization of the ligand-binding domain 25 (LBD) of the androgen receptor (AR). These mice exhibit an external female phenotype with undescended testes. Histological analysis of the testes of ARLmon/Y mice revealed some residual spermatogenesis. Hormone measurements in serum of the ARLmon/Y mice showed significantly high levels of androgens. To study if there is any remaining AR activity, we orchidectomized WT and ARLmon/Y mice and gave them supraphysiological testosterone replacement for 4 days. RNA-seq was performed on kindeys. Overall design: 5 samples per group: WT + Vehicle, WT + testosterone, ARLmon/Y + Vehicle, ARLmon/Y + testosterone