DRP004024
RAD sequence-based phylogeny of the periodical cicadas Magicicada
To reveal the phylogenetic relationships among broods of the periodical cicadas of the genus Magicicada, restriction-site associated DNA (RAD) sequences data were obtained for 64 adult specimens. Total genomic DNA of each individual was digested with SbfI, and 101-bp fragments including 5-bp barcodes for identification of individuals were sequenced by Illumina HiSeq2000 sequencer.