PRJDB2446
Comparison of bacteria community revealed by deep 16S rRNA pyrosequencing in two naturally suppressive or conductive soils for banana Fusarium wilt disease
Banana Fusarium wilt disease, caused by the fungus Fusarium oxysporum f. sp. cubense race 4, is a vast disaster to banana production all over the world. Two banana orchards with naturally occurring Fusarium disease suppression after long-term continuous cropping were found in south and north Hainan Island, China, respectively. Bacteria communities from these two orchards and the adjacent banana orchards seriously infected with Fusarium wilt disease were compared using deep 16S RNA barcode pyrosequencing. Results showed that higher richness (Chao1 and ACE) and diversity indices, and a significantly negative correlation to disease incidence were observed in both suppressive soils within the same field site. Moreover, more OTU numbers were observed in two suppressive soils. Both heatmap and hierarchical cluster analysis showed bacteria communities membership and structure in suppressive soils differed to conductive soils. Phylum of Acidobacteria was significantly elevated whereas Bacteroidetes was reduced in suppressive soils and genera of Pseudomonas, Gp4, Chthonomonas, Tumebacillus and Gp5 were enriched whereas Gp2 depressed in suppressive soils. Furthermore, the enrichment of Pseudomonas, Gp4 and Gp5 and the soil physicochemical property of pH and available P was positively correlated with disease suppression. In conclusion, this study characterized bacterial communities and chemical properties in two naturally suppressive soils and identified novel bacterial taxa that may be involved in suppression of banana Fusarium wilt disease.