GSE172473
miR-146a mediated Target Dependent Cooperative Biogenesis of miRNAs in Macrophage Cells
TLR4-activated immune response, elicited by exogenous or endogenous ligands, does give rise to several acute and chronic diseases, and plays a critical role as an amplifier of the inflammatory response. Along with, a cluster of TLR4-responsive miRNAs create a crosstalk during inflammatory escalation. Our data shows miR-146a even not having binding sites for different signalling mediators, alleviates pro-inflammatory response by modulating different stress-induced response (cytokine production, generation of NO, apoptosis etc) to reestablish macrophage homeostasis. We were curious to unravel this phenomenon. Here, we have reported miR-146a influences the biogenesis of a group of miRNAs after its overexpression. We have adopted a tet-on responsive miR-146a overexpression system in murine macrophages. A group of miRNAs has shown significant differential expression after miR-146a overexpression. Later, our targetome-based study correlated with this observation to establish the link between augmentations of anti-inflammatory response inside the cells with the miR-146a overexpression that probably have a significant role in establishing cellular homeostasis. Overall design: miR-146a overexpression in RAW264.7 cells regulates expression of other miRNA (Co-ordinate biogenesis)