ChIP-seq_of_PolII_unmodified_CTD_and_Ser5ph_in_mESC_Wild_Type_and_Setd5_Hetero_and_Homo_expid_600
"ChIP-seq of PolII unmodified CTD and Ser5ph in mESC Wild Type and Setd5 Hetero and Homo"
ChIP-seq for RNA Polymerase II with an antibody recognising unmodified CTD and Serine 5 Phosphorylation CTD (clone 4H8). For Each replicate of 100 million ESCs were harvested and cross linked in 1.5mM EGS and 1.5% PFA digested with MNase and mildly sonicated to achieve mono-di-tri nucleosome fragmented chromatin. Antibody: CST 2629, Lot No 3 ref 10/2017
ChIP-seq for RNA Polymerase II with an antibody recognising unmodified CTD and Serine 5 Phosphorylation CTD (clone 4H8). For Each replicate of 100 million ESCs were harvested and cross linked in 1.5mM EGS and 1.5% PFA digested with MNase and mildly sonicated to achieve mono-di-tri nucleosome fragmented chromatin. Antibody: CST 2629, Lot No 3 ref 10/2017