PRJNA758110
Long read nanopore sequencing  mismatch repair deficient related alternative splice in colorectal cancer
We applied long read nanopore sequencing to determine the mRNA profiles and screen AS genes associated with mismatch repair deficient (dMMR)/microsatellite instability (MSI) in 20 CRC tissues. Oxford Nanopore Technologies (ONT) method was carried out to construct full-length transcriptome, AS events were identified and the transcripts were quantified to calculate differential expressed genes between cancer and normal tissues.