GSE112095
Role of SmcHD1 in the establishment of the epigenetic states required for the maintenance of X chromosome inactivation [RNA-seq]
It has been shown that functional deficiency of SmcHD1, a noncanonical member of SMC family proteins, results in derepression of X-inactivated genes in postimplantation female mouse embryos. In this study, we found that derepression of X-inactivated genes accompanied a local reduction in the enrichment of H3K27me3 in mouse embryonic fibroblasts (MEFs) prepared from female fetuses deficient for SmcHD1. Overall design: Allele-specific RNA-seq analysis of mouse embryonic fibroblasts (MEFs) prepared from female fetuses deficient for SmcHD1. The MEFs were obtained from B6-JF1 F1 males crossed with B6 females carrying Xist-deltaA mutation.