GSE131394
Comparative transcriptome analysis of artificially induced rough-type Brucella strain RM57 and its parent strain
Purpose: To uncover the related mechanisms underlie virulence attenuation of RM57 vaccine candidate. Methods: Three Brucella melitensis M1981 strains and three Brucella melitensis RM57 strains were grown in TSB at 37? until the log phase was reached, total RNA was isolated using the TRIzol according to the manufacturer's instructions.The sequencing library of each RNA sample was prepared by using NEB Next Ultra Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer.The HiSeq 4000 platform was used to perform the transcriptome sequencing. Results: The results revealed that expressions of 1205 genes were significantly different between RM57 and M1981. Data analysis showed that in the gene ontology term, the different expressed genes involved in energy production and conversion, and translation, ribosomal structure and biogenesis were significantly enriched. Pathway enrichment analysis indicated that the genes involved in oxidative phosphorylation, ribosome, nitrogen metabolism, tyrosine metabolism and two-component system were significantly affected. Overall design: mRNA profiles of Three Brucella melitensis M1981 strains and three Brucella melitensis RM57 strains were generated by deep sequencing