GSE183843
Loss of FBXO11 establishes a stem cell program in acute myeloid leukemia by dysregulating LONP1 [RNA-seq]
Purpose: The goals of this study are to compare the transcriptome profile of human cord blood derived CD34+ cells expressing shRNA targeting FBXO11, LONP1 or non-targeting shRNA, combined with or without over-expression of FLAG-FBXO11 or LONP1 by RNAseq. Methods: mRNA profiles of CD34+ cord blood cells with combinations of FBXO11 or LONP1 knockdown or over-expression were generated by RNAseq. Overall design: CD34+ cord blood expressing non-targeting shRNA, shRNA targeting human FBXO11, shRNA targeting human LONP1, empty vector, FLAG-FBXO11, LONP1, or a combination of one of the shRNAs with the over-expression of FLAG-FBXO11 or LONP1.