GSE104546
Expression profilings of fission yeast strains (RNA-seq data set)
We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, clr6-1, ago1?, red1?, rrp6?, clr4?, ccr4?, ccr4?fep1, wild-type cells treated with an iron chelator (2,2'-bipyridyl; DIP) grown at 30°C or 18°C and ccr4?fep1 cells treated with DIP at 18C. We find that many stress response genes, transmembrane transporters, and non-coding RNAs are up-regulated in cells cultured at 18°C.  Our analyses concluded that Clr4 and Ccr4 are important for controlling transcript levels at 18°C and uncovered a role for iron homeostasis in adaptive genome control. Overall design: RNA sequencing in wild-type, clr6-1, ago1?, red1?, rrp6?, clr4?, ccr4?, ccr4?fep1, wild-type cells treated with an iron chelator (2,2'-bipyridyl; DIP) grown at 30°C or 18°C and ccr4?fep1 cells treated with DIP at 18C. Two biological replicates for wild-type strains grown at 30°C and 18°C.