T535A-sc-3200
T535A
EpiCS were transfected with KLF4 and different TET1 constructs. Cells were grown in standard N2B27 medium and collected after two passages. RNA was collected using All prep Qiagen kit and ran on gel to check for RNA integrity.
The aim of this study is to characterize the transcriptional profiles of EpiSC overexpressing wt TET1 versus T535A TET1 mutant.