GSE121561
RNA-seq in scraped colon epithelium after deletion of Synbindin in mice in colitis-associated cancer model
We previously identified Synbindin promoted gastric and colon cancer development by activating ERK on the Golgi in vitro. Advanced in vivo evidence is required.In this study, we genetically manipulated Synbindin expression in the intestinal epithelium using loss-of-function mouse models and subject them to AOM/DSS induced colitis-associated cancer model. At the end of the model, mice were sacrificed and colon tumors and adjacent colon epithelium were scraped and used for RNA-seq. Overall design: Colon mRNA profiles of Synbindinflox and Synbindin?IEC mice were generated by deep sequencing, in triplicate,using illumina Hiseq.