GSE159745
A single cell transcriptome atlas of Human thymic stromal compartment
Purpose: The goal of this study is to characterise the large variety of stromal cells populations, particularly cortex (cTECs) and medullary (mTECs) epithelial cells. It has been speculated that mTECs and cTECs are composed of functionally distinct subsets with different clonogenic potential. Methods: FACS-isolation protocol to separate 4 different epithelial cells populations from total dissociation of human thymus that is negative for hCD45 then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study dissected common clusters to human thymic epithelial cells in cortex and medulla containing stem cells markers conserved when expanded in vitro, as well as identified specialised cell clusters in each of the two compartment Overall design: 4 FACS sorted epithelial populations were used for single-cell RNA-sequencing (10xGenomics Chromium Platform), and sequenced using Illumina HiSeq 4000.