ena-STUDY-COMPLUTENSE UNIVERSITY-27-02-2017-11:45:17:901-183
Bacterial Diversity Of The Gastric Content Of Preterm Infants During Their NICU Stay
Gastric microbiota, because of its drastic conditions was classically the missing point in the study of the gastrointestinal tract. Despite recent studies described a complex gastric microbiota in adults, little is known concerning neonates.  Thirteen preterms infants with a mean gestational age of 28 weeks and a birth weight   of   1192   g  provide   gastric   content   samples   for  its   microbiological description using culture dependent and independent techniques. Bacterial  growth  was  detected  in  the  79%  of  the  gastric  content  samples. Dominant    species    found    by    culture    techniques    were Staphylococcus epidermidis, Enterococcus faecalisand Serratia marcescens. Clinically relevant isolates  were  typed  by  MLST  analysis  and  revealed  the  presence  of  strains belonged  to  high  risk  clonal  complexes  in  the  gastric  microbiota  of  preterms. Molecular  analysis  unveiled  a  phylogenetically  diverse  microbiota.  The  same dominant genera conforming gastric contents were found in feeding samples. In that  way,  minority  genera  such  as Mycoplasma,  Balneimonas, Aerococcus, Raoultella,Caulobacter; Shewanella and  Facklamia were  described  as  the autochthonous microbiota of studied infants.   Both,   culture   and   molecular   techniques   remarked Proteobacteria   as   the predominant  phyla in  gastric  content  samples  pointing  to  hospital  environment as  an  important  factor  modulating  preterm  microbiota  at  least  during  their hospital stay. Further work is required to better understand gastric colonization process  and  establish  whether  this  information  can  be  used  to  improve  infant outcomes.
Gastric microbiota, because of its drastic conditions was classically the missing point in the study of the gastrointestinal tract. Despite recent studies described a complex gastric microbiota in adults, little is known concerning neonates.  Thirteen preterms infants with a mean gestational age of 28 weeks and a birth weight   of   1192   g  provide   gastric   content   samples   for  its   microbiological description using culture dependent and independent techniques. Bacterial  growth  was  detected  in  the  79%  of  the  gastric  content  samples. Dominant    species    found    by    culture    techniques    were Staphylococcus epidermidis, Enterococcus faecalisand Serratia marcescens. Clinically relevant isolates  were  typed  by  MLST  analysis  and  revealed  the  presence  of  strains belonged  to  high  risk  clonal  complexes  in  the  gastric  microbiota  of  preterms. Molecular  analysis  unveiled  a  phylogenetically  diverse  microbiota.  The  same dominant genera conforming gastric contents were found in feeding samples. In that  way,  minority  genera  such  as Mycoplasma,  Balneimonas, Aerococcus, Raoultella,Caulobacter; Shewanella and  Facklamia were  described  as  the autochthonous microbiota of studied infants.   Both,   culture   and   molecular   techniques   remarked Proteobacteria   as   the predominant  phyla in  gastric  content  samples  pointing  to  hospital  environment as  an  important  factor  modulating  preterm  microbiota  at  least  during  their hospital stay. Further work is required to better understand gastric colonization process  and  establish  whether  this  information  can  be  used  to  improve  infant outcomes.