ESC_Methylome_Dynamics-sc-2013-04-17T14:08:04Z-2602
ESC_Methylome_Dynamics
Mouse Embryonic Stem Cells (ESCs) are grown in culture and seperated based upon heterogeneous expression of key pluripotency factors. DNA was obtained from cells with the Qiagen AllPrep kit. 0.2ug of DNA was sonicated with the Covaris Sonicator (300bp) and the Illumina library was prepared following a standard Illumina protocol with the NEB Next kit. The library was amplified 15x and size-selected within the range of 200-500bp.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The aim of this study is to investigate whether DNA methylation may have a role in controlling the dynamic equilibrium of ESCs. The study is going to involve a complete genome-wide and locus-specific analysis of differences in methylation between ESC sub-populations, including both mappable and unmappable genomic regions.