PRJNA767196
The RNA-seq datasets of monocot and eudicot plants under cold stress (4 degrees centigrade) at 0, 2, 24, and 168 hours (h).
We performed RNA-seq experiments with the leaf tissues from five monocots (Setaria italica, Zea mays, Hordeum vulgare, Oryza sativa, and Phyllostachys edulis) and six eudicots (Betula pendula, Carya illinoinensis, Glycine max, Cucumis sativus, Populus trichocarpa, and Arabidopsis thaliana) subjected to cold treatment (4 degrees centigrade) for 0, 2, 24, and 168 hours (h). Before cold stress treatment, all the plant seedlings were cultured in an artificial climate chamber with 25 degrees centigrade at a photoperiod of 16/8 h light/dark cycle. For A. thaliana, three-week old seedlings were used for cold stress treatments. For other species, young seedlings growing up to ~30 cm in height were utilized. Total RNA of the leaves collected from each species was isolated, purified, and assessed by NanoDrop ND-1000 and Bioanalyzer 2100. Poly(A)-RNA was enriched for cDNA library construction and sequencing by Illumina NovaSeq 6000 (2 x 150 bp paired-end reads).