ena-STUDY-INRA (INRA Jouy en Josas)-07-04-2017-13:05:18:722-29
Study of the mucosa associated microbiota in colorectal cancer patient with or without IBD
Gut microbiota dysbiosis has been associated with inflammatory bowel diseases (IBD). In colorectal cancer, the gut microbiota has also been recognized as potentially involved in aggravating or favoring the tumor development. In both diseases, specific microorganisms have been identified suggesting potential therapeutic avenues. However, very little is known on the structure and role of the microbiota in colitis associated cancer (CAC), an important complication of IBD in human. Here we analyzed the bacterial and fungal composition of the mucosa associated microbiota of patients suffering CAC, sporadic cancer (SC) and of healthy subjects (HS). Our prospective, observational study evaluated bacterial and fungal composition of mucosa-associated microbiota of 7 CAC patients, 10 SC patients and 10 HS using 16S (MiSeq) and ITS2 (pyrosequencing) sequencing, respectively.
Gut microbiota dysbiosis has been associated with inflammatory bowel diseases (IBD). In colorectal cancer, the gut microbiota has also been recognized as potentially involved in aggravating or favoring the tumor development. In both diseases, specific microorganisms have been identified suggesting potential therapeutic avenues. However, very little is known on the structure and role of the microbiota in colitis associated cancer (CAC), an important complication of IBD in human. Here we analyzed the bacterial and fungal composition of the mucosa associated microbiota of patients suffering CAC, sporadic cancer (SC) and of healthy subjects (HS). Our prospective, observational study evaluated bacterial and fungal composition of mucosa-associated microbiota of 7 CAC patients, 10 SC patients and 10 HS using 16S (MiSeq) and ITS2 (pyrosequencing) sequencing, respectively.