GSE120209
Screening of the targeted mRNAs of ER-anchored PARN by transcriptome sequencing.
The goals of this study are to identify the potential targets and then elucidate the function of ER-anchored PARN. After overexpressing the empty vector, PARN(wild type) or D28A(the enzyme-dead mutant of PARN) in HEK-293T cell respectively and the following fractionation of the cytosol and ER fraction, we performed the high-throughput RNA sequencing for each fraction. After comparing the abundance of detected transcripts in different samples we found ER-anchored PARN only affects a small subset of mRNAs in HEK-293T cell. Overall design: HEK-293T mRNA profiles of cytosol and ER fraction after overexpressing pcDNA3.1(N- flag,empty vector),flag-PARN and flag-PARN-D28A