GSE175627
Ectopic expression of Sox9 led to differential gene expression in HepG2 cells
We generated the HepG2 subline stably expressing Sox9 (HepG2-Sox9) and performed transcriptome RNA sequence. Overall design: Total RNA was extracted from HepG2 subline cells with TRIzol reagent. The RNA purity and integrity were analyzed with the Agilent Bioanalyzer 2100 (Agilent). The mRNA was purified from total RNA after removal of rRNA and sequenced on an Illumina Hiseq2000 system.