GSE173333
ChIP-Seq Files : The Hypoxia Response Pathway Promotes PEP Carboxykinase And Gluconeogenesis In C. elegans
To identify genes directly regulated by HIF-1, we performed ChIP-seq on L4-stage egl-9(sa307); hif-1(ia4); odIs131[hif-1::gfp] animals, which have activated HIF-1 under aerobic conditions, using anti-GFP antibodies to precipitate DNA bound by HIF-1::GFP followed by high-throughput sequencing to identify peaks of reads corresponding to HIF-1 binding sites Overall design: ChIP-seq on OR3349 (odIS131[hif-1::gfp) control animals and OR3350 (egl-9(sa307); hif-1(ia4); odIs131[hif-1::gfp]) under 21% O2, NGM media with OP50-1 as food