ena-STUDY-UNAM-06-04-2018-00:05:29:398-1667
Transcriptome analysis of the venom gland of Paravaejovis schwenkmeyeri by RNA-seq
The telsons of four specimens of the Mexican scorpion Paravaejovis schwenkmeyeri were dissected, from which total RNA was extracted. A cDNA library was constructed that was further subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 8,858,816 reads were obtained, which assembled into 77,108 putative transcripts. From them, 44,043 where identified matching gene sequences in databases. A subgroup was annotated, of which 6,979 matched known arthropod genes. A total of 549 putative transcripts were identified with sequence similarity to annotated genes from arachnids, 289 of which corresponded specifically to scorpions.
The telsons of four specimens of the Mexican scorpion Paravaejovis schwenkmeyeri were dissected, from which total RNA was extracted. A cDNA library was constructed that was further subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 8,858,816 reads were obtained, which assembled into 77,108 putative transcripts. From them, 44,043 where identified matching gene sequences in databases. A subgroup was annotated, of which 6,979 matched known arthropod genes. A total of 549 putative transcripts were identified with sequence similarity to annotated genes from arachnids, 289 of which corresponded specifically to scorpions.