PRJNA491203
Mutant allelic series generated in the Trp53 ICE CRIM mouse model
We generated a transgenic inducible Cas9 effector/CRISPR mutagen (ICE CRIM) mouse model, in which CRISPR/Cas9-mediated somatic mutagenesis events can occur in response to a Cre expression. The well-known tumor suppressor gene, Trp53, and two important DNA mismatch repair genes, Mlh1 and Msh2, were selected to be our somatic mutagenesis targets. Amplicon-based deep sequencing of sgRNA-target site(s) was performed to validate the editing efficiency and to identify mutant allelic series.