GSE173410
A novel transcription factor PunR and Nac are involved in purine and purine nucleoside transporter punC regulation in E. coli
Recently, novel analytic methods have divided bacterial transcriptomes into independently modulated sets of genes (iModulons). Functionally annotated iModulons that contain y-genes lead to testable hypotheses to elucidate y-gene function. The inversely correlated expression of a putative transporter gene, ydhC, relative to purine biosynthetic genes, has led to the hypothesis that it encodes a purine-related transporter and revealed a LysR-family regulator, YdhB, with a predicted 23-bp palindromic binding motif. RNA-Seq analysis of a ydhB knockout mutant confirmed the YdhB-dependent activation of ydhC in the presence of adenosine. The deletion of either the ydhC or the ydhB gene led to a substantially decreased growth rate for E. coli in minimal medium with adenosine, inosine, or guanosine as the nitrogen source. Taken together, we provide clear evidence that YdhB activates the expression of the ydhC gene that encodes a novel purine transporter in E. coli. We propose that the genes ydhB and ydhC be re-named as punR and punC, respectively. Overall design: The wild type MG1655 strain was grown as a control for the isogenic punR mutant strain. The cells were grown in M9 glucose medium supplemented with 2.5 mM adenosine and collected at an OD600 of 0.6 and were harvested using the Qiagen RNA-protect bacterial reagent according to the manufacturer's specifications.