ena-STUDY-INRA (Nouvelle-Aquitaine-Bordeaux)-28-03-2019-14:11:35:191-146
Sequence based microsatellite genotyping development for Melipona variegatipes.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach in Melipona variegatipes. A total of 54 loci were amplified in a multiplexed PCR and sequenced for 91 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 39 high quality loci were validated.
New microsatellite markers have been optimized for the development of a sequence based microsatellite genotyping approach in Melipona variegatipes. A total of 54 loci were amplified in a multiplexed PCR and sequenced for 91 repeated individuals to estimate genotyping error. Data were used as a proof of concept of the method and 39 high quality loci were validated.