GSE157791
Co-culture with mouse embryonic fibroblasts improves maintenance of metabolic function of human small hepatocyte progenitor cells
Small hepatocyte progenitor cells (SHPCs) can be derived from primary adult human hepatocytes on mouse embryonic fibroblasts (MEFs). SHPCs derived and cultured on MEFs retain their differentiated morphology and gene expression profiles over several passages. SHPCs in MEF co-culture metabolize acetaminophen to all of its major metabolites at rates equal to mature adult primary human hepatocytes. Overall design: Examination of SHPC co-culture on MEFs for better retention of metabolic functions