GSE115921
ChIP-Seq: Unstimulated and BMP9/10 Stimulated Endothelial Cells
To identify potential biological targets of the TGFß pathway involved in AVM formation, we performed ChIP-sequencing experiments on BMP9 stimulated Ms1 endothelial cells (ECs). This data provides a comprehensive list of all SMAD4 binding sites in endothelial cells. Overall design: We sequenced a total of 8 samples for this experiment. For the nonstimulated dataset, we have 2 input samples and 2 Smad4 IP samples. The SMAD4 antibody used for immunoprecipitation was Cell Signaling 46535. The BMP9/10 used for stimulation was obtained through R&D catalog #5566 and #6038 (see publication for further information). Likewise for the stimulated dataset, we have two input samples and two SMAD4 IP samples.