ena-STUDY-ENS Lyon-09-03-2017-14:54:18:504-297
Mapping-by-sequencing data for mutant allele mf89 (Oscheius tipulae)
The Oscheius tipulae strain JU172 carries the recessive mutation mf89 in the CEW1 background (reference genome). In order to map and identify this mutation, It was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from several independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.
The Oscheius tipulae strain JU172 carries the recessive mutation mf89 in the CEW1 background (reference genome). In order to map and identify this mutation, It was crossed with the mapping strain JU170. Genomic DNA was extracted and pooled from several independent mutant F2 lines and sent for sequencing on a Illumina Hiseq 2000 plateform to generate 100-bp paired-end reads.