GSE192613
The effect of 22-bulge of pre-miRNAs on DICER cleavage in human cells.
The experiments were conducted to examine the effects of 22-bulge of pre-miRNAs on DICER cleavage in human cells. Overall design: HCT116 cells were transfected with pCDNA3 plasmids expressing pri-miRNA-216a containing 22-3p-bulge or 22-5p-bulge or 22-mismatch or nobulge. Small RNA sequencing libraries of the transfected HCT116 cells generated using NEBNext® Small RNA Library Prep Set for Illumina® (NEB).