PATCH_MICROBIOME-sc-4865
PATCH_MICROBIOME
This study addresses the role of the intestinal microbiome in susceptibilty to Salmonella Typhi and Salmonella Paratyphi A in a human challenge model. Briefly, volunteers are challenged with 10(4) S. Typhi or 10(3) S. Paratyphi Ai and monitored over a two-week period for clinical signs of typhoid fever. Approximately sixty percent of challenged volunteers develop typhoid fever. All volunteers receive antibiotic treatment. The study takes fecal samples from challenged individuals and studies microbiome composition day 0, 7, 14, 28 and 90 post challenge.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
The study aims to address changes in microbiome composition during human experimental challenge with S. Typhi and S. Paratyphi A. An additional objective is to address changes in microbiome composition during secondary challenge. The data genererated should inform further studies relating to response of the normal microbiota during challenge with an enteric pathogen and the response of the the microbiota to antibiotic treatment against the challenge organism. The ultimate goal is to extend these findings to design similar studies to areas where typhoid is endemic and antibiotic therapy is widely used.