GSE106252
RNA pol II ChIP-seq LR14 and HFF human fibroblasts
The aim of the experiment is to determine if RNA pol II occupancy and abundance is altered human fibroblasts from a patient that has a mutation in the gene Taf8, which plays an important role in RNA pol II mediated transcription. Overall design: I performed ChIP with an antibody against RNA pol II, and have submitted the precipitated DNA from patient and control samples (L-IP and H-IP, respectively). In order to aid with normalisation, I spiked the fibroblast chromatin with 1/10 drosophila chromatin. Input for the HFF sample will also be submitted.