DRP007094
Single cell RNA-sequencing of mouse osteoblasts
Osteoblasts have been described to exhibit heterogeneous expression of a variety of osteoblast markers at both transcriptional and protein levels. To explore further this heterogeneity and its biological significance, we performed single cell RNA-sequencing on freshly isolated osteoblasts. These osteoblasts were isolated from newborn mouse calvariae as Venus-positive cells expressing the fluorescent protein Venus under the control of the 2.3-kb Col1a1 promoter. Two hundred and eighty-five single-cell libraries and control libraries were successfully collected and sequenced.