GSE124429
A runaway PRH/HHEX-Notch3 feedback loop drives cholangiocarcinoma (RNA-Seq)
PRH/HHEX-Notch3 feedback loop drives cholangiocarcinoma Overall design: 16 samples total. 3 experiments: (i) PRH shRNA knockdown vs scrambled control. 2 independent clones expressing each shRNA (4 samples total). (ii) PRH overexpression using myc-PRH adenovirus vs empty adenovirus. 2 independent infections with each virus (4 samples total). (iii) Overexpression of PRH in Notch3 KD and control shRNA cell lines using myc-PRH adenovirus. (8 samples total - 2 independent infections each for all combinations of Notch3/control shRNA and myc-PRH/empty adenovirus)