ena-STUDY-CNRS-09-12-2017-15:05:07:051-56
A multiscale approach to decipher molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti
A laboratory strain of Ae. aegypti (Liverpool strain) was exposed to ibuprofen (1µg/L)from larvae hatching to imagoes emergence. To understand the mechanistic processes underlying the direct impacts of ibuprofen on exposed larvae, we used RNA-seq based transcriptomic approach. Each condition (exposed or not to ibuprofen) was analyzed in triplicate, each RNA sample being extracted from L4 stage larvae. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads.
A laboratory strain of Ae. aegypti (Liverpool strain) was exposed to ibuprofen (1µg/L)from larvae hatching to imagoes emergence. To understand the mechanistic processes underlying the direct impacts of ibuprofen on exposed larvae, we used RNA-seq based transcriptomic approach. Each condition (exposed or not to ibuprofen) was analyzed in triplicate, each RNA sample being extracted from L4 stage larvae. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads.