PRJNA577890
Sequencing of SIV Infected Macaca Mulatta Lymph Nodes
We sequenced 31 macaque lymph nodes from six animals using TrueSeq Stranded Total RNA with RiboZero Gold rRNA depletion with 200ng input. The macaque are infected with SIV. The RNA is not poly-a selected, so it includes the viral RNA as well as non-polyadenylated transcripts. The goal of the study is to support development of our spatial sequencing techniques. Supported by the Bill and Melinda Gates Foundation. Principle Investigators: Samuel A. Inverso of ReadCoor and Jacob Estes of the Oregon Health &amp; Science University