GSE191158
Transcriptional profiling of estrogen receptor fusion genes in breast cancer cell lines
Genomic analysis has recently identified multiple ESR1 gene translocations in estrogen receptor-alpha positive (ERa+) metastatic breast cancer (MBC) that encode chimeric proteins whereby the ESR1 ligand binding domain (LBD) is replaced by C-terminal sequences from many different gene partners. Here we functionally screened 15 ESR1 fusions and identified 10 that promoted estradiol-independent cell growth, motility, invasion, EMT and resistance to fulvestrant. RNA sequencing identified a gene expression pattern specific to functionally active ESR1 gene fusions that was subsequently reduced to a diagnostic 24-gene signature. This signature was further examined in 20 ERa+ patient-derived xenografts (PDXs) and in 55 ERa+ MBC samples. The 24-gene signature successfully identified cases harboring ESR1 gene fusions and also accurately diagnosed the presence of activating ESR1 LBD point mutations. Therefore, the 24-gene signature represents an efficient approach to screening samples for the presence of diverse somaticESR1 mutations and translocations that drive endocrine treatment failure in MBC. Overall design: RNA-seq of stable T47D breast cancer cells expressing HA-tagged ESR1 constructs and YFP control in hormone deprived media, -/+ E2. (ESR1-e6 denotes ESR1 fusion containing the first 6 exons of ESR1)