Identification_of_mutants_in_Xenopus_tropicalis_by_Exome_Sequencing_-sc-2011-04-08T11:42:45Z-1795
Identification_of_mutants_in_Xenopus_tropicalis_by_Exome_Sequencing_
Genomic DNA is obtained from a library of Xenopus tropicalis that have been randomly mutagenised with ENU.  Agilent SureSelect exon enrichment is used to pull down the exome for Illumina sequencing.  Mutations are identified and nonsense and splice mutants will be analysed for phenotypes.  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
In order to further exploit Xenopus tropicalis as a genetic model we have generated a library of animals that have been randomly mutagenised with ENU.  We are using reverse genetics to identify nonsense and splice mutations.  By sequencing the exome of each individual animal we hope to identify the full complement of alleles in each mutant.  This data will be shared via Ensembl and the mutant lines will be available on request.