PRJNA252566
Thunnus thynnus thynnus Transcriptome or Gene expression
Sequencing and characterization of the Atlantic bluefin tuna transcriptome aimed at SNP discovery and providing a novel genomic resource. Pooled cDNA libraries constructed with RNA extracted from 6 adult individuals and 14 developmental stages were sequenced on a an Illumina HiSeq2000. The 215 millions of paired-end reads obtained were assembled in a draft transcriptome comprised of 37,590 contigs ranging in size from 201 to 27,825 bp. SNP calling resulted in the identification of 147,534 SNPs. The genomic resource obtained will enable development of targeted assays for large numbers of polymorphic genetic markers.