GSE183430
Transcriptomic analysis of Brm-/- hematopoietic stem and progenitor cells (LSK)
Little is known of hematopoietic stem (HSC) and progenitor (HPC) cell self-renewal. The role of Brahma (BRM), a chromatin remodeler, in HSC/HPC function is unknown. Bone marrow (BM) from Brm-/- mice manifested increased numbers of long- and short-term HSCs, GMPs, and increased numbers and cycling of functional HPCs. Brm-/- HSC/HPC show demonstrated functional differences compared to wildtype HSC/HPC in vivo and ex vivo, due in part to increased intracellular valine in lineage negative BM. To determine changes in gene programs associated with loss of BRM, we performed RNA sequencing on Brm-/- LSK cells. Brm-/- LSK exhibited upregulated interferon response/cell cycle gene programs, suggesting important extrinsic effects on primitive HSC/HPC from more mature HPC due to the absence of BRM. Overall design: Bone marrow cells were harvested from wiltdype (n=3) or Brm-/- (n=3) mice. Lineage negative, CKIT+, SCA1+ (LSK) cells were sorted from each population using cell surface staining followed by flow cytometry. Cells were immediately lysed RNA was harvested and prepped for sequencing.