PRJNA261262
Genotyping by sequencing (GBS) of V. rupestris B38 x Vitis vinifera ''Chardonnay''
Improved efficacy and durability of powdery mildew resistance can be enhanced via knowledge of the genetics of resistance and susceptibility as well as high resolution maps to facilitate the stacking of multiple resistance genes and other desirable traits. We studied the inheritance of resistance and susceptibility of wild Vitis rupestris B38 and cultivated V. vinifera ‘Chardonnay’ against grape powdery mildew (Erysiphe necator). Molecular markers were identified using Genotyping-by-Sequencing (GBS) to obtain a map of 16,833 Single Nucleotide Polymorphism (SNPs) based on alignment to the V. vinifera ‘PN40024’ reference genome sequence. With an average density of 36 SNP/Mbp and uniform coverage of the genome, this is the densest Vitis map published to-date and represents an improvement of at least 10-fold over current standards for grapevine linkage maps.