3'UTR sequences of D.labrax
Characterization of European Sea Bass transcripts by RNA SEQ after Oral Vaccine against V. anguillarum
In the present study we performed RNA seq in two different tissues; gut and head-kidney. For each tissue one control sample (where a sample presents a pool of 4-5 individuals) and one sample after oral vaccine against V. anguillarum were submitted to 454 next generation sequencing. Synthesis of first strand cDNA was performed using oligo nucleotide primer 5’-TTTTTTCTTGTTTTCTTTTCTTV-3’ and cDNA library protocol of GSFLX454 was adapted accordingly.