DRP005363
Effects of FujiFF on microbiota profiling in the feces of the mice fed a high fat-high sucrose diet
Four weeks old Male C57BL/6 mice were fed a cellulose- or FujiFF-containing high fat-high sucrose diet for 12 weeks. Fecal droppings were collected at the end of treatment. Fecal DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces (MP Biomedicals, Santa Ana, CA) per the manufacture's instructions. The V4 region of the 16S rRNA gene was amplified using dual-indexed 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with a MiSeq Reagent 222 kit V3 (Illumina, USA). The libraries were prepared following the Illumina protocol for 10pM: 'Preparing Libraries for Sequencing on the MiSeq' (part 15039740, Rev. D).