GSE114856
RNA sequencing results of EMT and CSC phenotypes induced by modulated expression of wild-type and mutated PSPC1 and PTK6
We found that PSPC1 is the master activator for transcription factors of EMT and stemness and accompanies c-Myc activation to facilitate tumour growth.  To elucidate mechanisms that the underlying post-translational modification of PSPC1, we performed the RNA-seq analysis of overexpression of PSPC1, alone and with PTK6, and PSPC1 tyrosine residue mutation in SK-Hep1 cells. We performed c-terminal of PSPC1 and its mutated nuclear localization site (NLS) in Mahlavu cells to evaluate its inhibitory effects on gene expression. Moreover, we further treated with HGF in Huh-7 cells to examineThe role of downstream signaling pathways of the PSPC1 and PTK6 regulation in microenviroment. Overall design: Gene expression profiles of PSPC1 with modulated overexpression in SK-Hep1 cells and Mahlavu cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells. Gene expression profiles of HGF treatment in Huh-7 cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells.