DRP004659
Microbiota of incubated salivary microbiome
Salivary samples of 2 healthy subjects were collected at 10:30 and a part of them were incubated to be sampled every hour until 16:30. Fresh salivary samples were also collected concurrently. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R) and, multiplexed amplicon pyrosequencing was carried out using 454 GS FLX Titanium or 454 GS JUNIOR (Roche Applied Science).