HCA_Bone_Foetal_WSSS_RNA_SB-sc-6068
HCA_Bone_Foetal_WSSS_RNA_SB
Haematopoeisis emerges in the human fetal bone marrow (BM) at around 12 post conception weeks.  This constitutes the second wave of definitive haematopoiesis following on from the first wave in fetal liver from around 6 post conception weeks.  The BM emerges as the dominant site of haematopoiesis, responsible for lifelong blood and immune cell production.  Yet, little is known about how the composition of the fetal BM, the microenvironment permissive to establishing haematopoiesis and the interplay with other sites of fetal haematopoiesis in fetal health or disease.  Herein we utilise single cell RNA sequencing to describe fetal haematopoeisis throughout gestation and better understand the development of the human immune system. The raw and processed fetal bone marrow 10x scRNA-seq data for this study are deposited at ArrayExpress or European Nucleotide Archive, with accession codes as follows: E-MTAB-9389 and ERP125305. Related accession codes for this study (including Smart-seq2 and CITE-seq data) are noted in the manuscript and linked to the main accession at E-MTAB-9389.
The Human Cell Atlas initiative is an ambitious global research effort which aims to describe every cell in the human body across all developmental stages to generate a reference map to accelerate progress in science and medicine particularly relating to human development and disease. The initiative is led by investigators based at the Wellcome Sanger Institute and the Broad Institute of MIT and Harvard and funding support from the Wellcome and NIH. This specific project will assess human development. We aim to interrogate the cellular composition and molecular regulators underpinning development and maturation through single cell RNA-sequencing/spatial transcriptomics and computational algorithms to predict cellular developmental trajectories and cell-cell interactions. We will subsequent validate these findings in situ using allied imaging technologies e.g. immunohistochemistry and RNAscope and in vitro culture of human intestinal organoids (HIOs).  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/