GSE124346
Analysis of transcriptome profiling of somatic and reproductive cells under UV-B irradiation in the green alga Volvox carteri
Purpose: Light is one of the most important environmental signal that affects the physiology and action of Volvox carteri. However, there are limited reports about the effects of UV-B irradiation on genome-wide transcriptional regulation in this organism. We surveyed the overall transcriptional responses of somatic and reproductive cells to UV-B irradiation using RNA-seq data. Methods: The somatic and reproductive cells were separated. The cell-types were treated for one hour by low-doses UV-B irradiation. Three biological replicates per cell-types (totally 12 samples) were used for RNA extraction. High-throughput RNA sequencing performed with separated-cells samples. Sequencing runs were performed on an Illumina HiSeq-2500. Transcriptome analysis was carried out in order to elucidate the effect of UV-B irradiation on whole transcriptional modification of physiological mechanisms. Results: In total, 264 and 272 million clean reads were produced in somatic and reproductive cells, respectively. The results showed that, as compared to control group, there were no differentially expressed genes in reproductive cells under treatment. However, treating the somatic cells with UV-B irradiation resulted in 126 differential genes as compared to untreated control group. Our results showed that there is light-specific transcriptional regulation in this organism. So that, different pathways were co-regulated by UV-B irradiation via the transcriptional regulation of genes encoding key enzymes in these pathways. Conclusions: Our findings showed that low UV-B irradiation influence on the cell-type specific gene expression. The findings of this study may present new insights to understand responsive mechanisms to UV-B irradiation by modulating expression of cell-type specific in the V. carteri. Overall design: Investigation of directed effects of UV-B irradiation on transcriptomic profiling of V. carteri.