GSE162845
Next generation sequencing  of  human cholangiocarcinoma cells and associated stem-like component
The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA).   Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided. Overall design: mRNA profile of 3D cholangio-spheres (SPH) compared to parental 2D monolayer (MON) cells of 4 different cholangiocarcinoma cell lines