UPSC-0129
Arabidopsis thaliana short-term cold stress data set
Short-term exposure of Arabidopsis thaliana to low non-freezing temperatures results in a cold tolerance increase by a phenomenon known as cold acclimation. The aim of this experiment was obtain short-term acclimation response. Arabidopsis thaliana seeds (ecotype Columbia-O) were sown into soil and kept in an 8 h photoperiod (150 micromol photons per square meter per second) at 23degree Celsius and after 14 days seedlings were transplanted into individual pots. After a further 30 days plants were shifted to 5 degrees Celsius and leaves of these plants were sampled after 0, 6, 24 hours and 3 and 10 days at 5 degrees Celsius and analyzed by RNASeq (control, 6h, 24h, 3d and 10d 5C respectively). For all samples leaves were harvested from 5-10 randomly chosen plants and these were pooled. Three pools were collected per time point to represent three biological replicates.
Short-term exposure of Arabidopsis thaliana to low non-freezing temperatures results in a cold tolerance increase by a phenomenon known as cold acclimation. The aim of this experiment was obtain short-term acclimation response. Arabidopsis thaliana seeds (ecotype Columbia-O) were sown into soil and kept in an 8 h photoperiod (150 micromol photons per square meter per second) at 23degree Celsius and after 14 days seedlings were transplanted into individual pots. After a further 30 days plants were shifted to 5 degrees Celsius and leaves of these plants were sampled after 0, 6, 24 hours and 3 and 10 days at 5 degrees Celsius and analyzed by RNASeq (control, 6h, 24h, 3d and 10d 5C respectively). For all samples leaves were harvested from 5-10 randomly chosen plants and these were pooled. Three pools were collected per time point to represent three biological replicates.