PRJNA268519
Homo sapiens Genome sequencing
Low-coverage single-cell whole-genome sequencing was performed on neurons from three normal and one pathological human brain (trisomy-18) as well as single cells from a normal lymphoblast cell line to identify somatic copy-number variants. Bulk and 10-cell lymphoblast as well as 100-neuron brain controls were also whole-genome sequenced as controls. A total of 138 single-cells were sequenced, DNA from 50 of the cells were prepared by genome amplification with GenomePlex (Sigma) and the DNA from the remaining 88 cells were prepared by genome amplification with multiple displacement amplification (MDA).