GSE124242
De novo formation in maize from irradiated materials
In maize (Zea mays), irradiation can produce many variants with varying centromeric DNA sequences. We found de novo centromeres in F3-152 and F3-600, which has no canonical centromeric repeat sequences. This centromere is derived from a 1520-kb and 188-kb region on the arm of chromosome 10 and 3. Our results suggest that de novo centromere formation is more common than previously thought and can persist on chromosomal fragments without a canonical centromere providing implications for karyotype evolution. Overall design: ChIP-seq was carried out with anti-CENH3 antibodies using material from young leaves in B73, F3-152 and F3-600.