ena-STUDY-UNIVERSITY OF OXFORD-03-06-2019-12:55:58:448-692
Metagenomic Nanopore sequencing of influenza virus direct from clinical respiratory samples
Detection of Influenza virus by application of Oxford Nanopore Technology to metagenomic sequencing of respiratory samples. Influenza reads limit of detection of 102-103 genome copies/ml in pooled samples, observing a strong relationship between the viral titre and the proportion of influenza reads. In clinical throat swabs, influenza reads detected from 27/27 samples with high-to-mid viral titres (Cycle threshold (Ct) values <30) and 6/13 samples with low viral titres (Ct values 30-40). No false positive reads were generated from 10 influenza-negative samples.
Detection of Influenza virus by application of Oxford Nanopore Technology to metagenomic sequencing of respiratory samples. Influenza reads limit of detection of 102-103 genome copies/ml in pooled samples, observing a strong relationship between the viral titre and the proportion of influenza reads. In clinical throat swabs, influenza reads detected from 27/27 samples with high-to-mid viral titres (Cycle threshold (Ct) values <30) and 6/13 samples with low viral titres (Ct values 30-40). No false positive reads were generated from 10 influenza-negative samples.