GSE162469
Pooled CRISPR Screening Identifies m6A as a Positive Regulator of Macrophage Activation [Amplicon-seq]
Customized pooled RNA Binding Protein (RBP) CRISPR libraries were applied to macrophage cell line Raw 264.7 cells to identify RBPs that are critical for LPS activated TNFa production.sgRNAs that enriched in TNFa-low, TNFa-High and pre-sort populations were sequenced and significant enriched genes were retrieved with MAGeCK test subcommand. Overall design: Examination of single guide RNAs (sgRNAs) enrichment in sorted cell populations based on TNFa expression