PRJNA391033
Plasmodium berghei transcriptome for PbSR-MG knock-out parasites: female gametocytes, male gametocytes, and asexual erythrocytic stages
Transcriptome sequencing data are derived from purified female and male gametocytes of Plasmodium berghei, and general erythrocytes as a reference. The parasites sequenced are P. berghei ANKA 820cl1m1cl1 PbSR-MG knock-out parasites. This is a transgenic line that expresses RFP and GFP under the induction of a female- and male-specific promoter respectively, and has subsequently had PbSR4 ablated. Gametocytes were sorted by flow cytometry, and general erythrocytic stages were depleted of host RNA by CF11 column treatment followed by saponin lysis. Samples were sequenced in biological triplicates.