DRP002432
Single-cell RNA-seq analysis of the cell cycle with HeLa Fucci cells.
The goal of these libraries is to find transcriptome signatures of the different phases of the cell cycle. Single HeLa "Fucci" cells, expressing fluorescent reporters that peak in G1 and G2/M phases, were captured in C1 Single-Cell Auto Prep systems (Fluidigm). After recording red and green fluorescence for each cell individually, each cell was lysed and their RNAs converted to cDNAs in the C1 systems using SMARTer kits (Clontech). The cDNAs were then collected and converted to RNA-seq libraries by tagmentation using Illumina/Nextera kits.
The goal of these libraries is to find transcriptome signatures of the different phases of the cell cycle. Single HeLa "Fucci" cells, expressing fluorescent reporters that peak in G1 and G2/M phases, were captured in C1 Single-Cell Auto Prep systems (Fluidigm). After recording red and green fluorescence for each cell individually, each cell was lysed and their RNAs converted to cDNAs in the C1 systems using SMARTer kits (Clontech). The cDNAs were then collected and converted to RNA-seq libraries by tagmentation using Illumina/Nextera kits.