GSE160726
Transcriptomic profiling of the liver from dnmt1 mutant zebrafish larvae compared to their phenotypically normal siblings at 120 hpf
In this dataset we performed RNA-seq on pools of livers dissected at 120 hpf from dnmt1(s904) mutants and phenotipically wild-type looking siblings. Overall design: dnmt1-s904 (ABNYU background) hetorozygous zebrafish adults were incross to generate larvae. We collected 20-30 livers from each experimental group from 3 independent clutches of dnmt1-s904 mutants and their phenotypically WT siblings which were sorted based on phenotype following immobilization using triacane at 120 hours post fertilizaion (dpf). Livers were manually dissected under the stereomicroscope and put directly in Trizol for the following extraction. Total RNA was DNAseI treated, depleted for ribosomal RNA by using RiboZero and used for Illumina Library prep.