GSE112928
ChIPseq in Mouse Hematopoietic Progenitor Cells (HPCs)
ChIPseq to detect binding of Nup98, SET1A to chromatin and to observe H3K4me3 on chromatin under various conditions Overall design: Extract DNA from BALB/c mouse HPCs, immunopurify protein complexed and associated DNA, deep sequence to identify genomic binding sites. Antibodies used: Nup98 (P671) - Cell Signaling Technology - #2292 H3K4me3 - Abcam - ab8580 SET1A - Abcam - ab70378 Flag - Sigma - Flag M2 affinity gel #A2220