DRP006532
Deep amplicon sequencing of 18S rRNA genes of Nematodes isolates.
We performed deep amplicon sequencing of 18S rRNA genes of Nematode isolated from the forest soils. A total of 96 isolates was individually subjected to amplicon PCR using Nematode specific primer sets targetting four different regions of 18S rRNA genes. The PCR products were then indexed and sequenced on the MiSeq instruments.