PRJNA866532
Decorating chromatin for enhanced genome editing using CRISPR-Cas9
To improve the efficiency of homology-directed repair of double-stranded breaks introduced by CRISPR-Cas9, a Cas9-PRDM9 (PR/SET Domain 9) fusion protein was generated and tested in HEK-293T cells. RNA-seq was used to quantify gene expression in the parental HEK-293T cell line.