GSE173296
Role of Cebpd on transcriptional output in 4T1 mouse triple negative breast cancer cells
Cebpd is a transcription factor regulating cytokines. We determined the role of Cebpd in the regulation of transciptional output. We generated 4T1 breast cancer cells with CRISPR/Cas9 mediated Cebpd knockout. We treated control sgRosa26 and sgCebpd cells with vehicle or IFNgamma (20ng/ml) for 24 hours and isolated RNA for sequencing. Overall design: Examination of transcriptome in Cebpd intact and Cebpd loss 4T1 breast cancer cells in response to IFNgamma.