DRP000034
Massive transcriptional start site mapping of mouse embryo 15days             cells.
Although recent studies have revealed that the majority of mouse genes             are subjected to regulation of alternative promoters (APs), the biological relevance of             this phenomenon remains unclear. To enable more comprehensive TSS analysis in the             respective cell types, we recently developed a method,combining oligo-capping with the             massively paralleled sequencing technology,Illumina GA. In this method, which we named             TSS Seq, sequence adaptor which is necessary for Illumina GA sequencing is directly             introduced to the cap site of the mRNA. By sequencing 36-48 sequence immediately             downstream of the TSSs (TSS tags), it is possible to obtain precise positional             information of transcriptional start sites (TSSs). 
Comprehensive identification and characterization of the transcriptional             start sites of human genes were carried out. For this purpose, we used our TSS-Seq             method, in which next gene sequencing technology and our full-length cDNA library             technology, oligo-capping were combined.