PRJNA535392
Transcriptome analysis and identification of insecticide metabolism related genes after exposure to insecticide in Sitobion avenae
Aphids causes serious lost in the production of wheat. Grain aphid, Sitobion avenae, is the dominant species of aphid in all wheat region of China, and this species is also considered resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and metabolize mechanism of insecticides for S. avenae is still unclear. This study employed transcriptome analysis to compare the expression patterns of stress-response genes under imidacloprid and chlorpyrifos for 15min, 3h and 36h'exposure. After compared insecticide-treated samples of different time duration to control sample, we obtained 60 to 2267 Differential Express Unigenes (DEUs), among these DEUs, 31-790 unigenes was classified into 66-786 categories of GO function group and 24 to 760 DEUs could be mapped into 54 to 268 KEGG pathways. The expression of DEUs related to insecticide-metabolism-related genes were analyzed. In the insecticide-metabolism-related genes, cuticle protein is the largest group in DEUs, and the second largest is ABC transporter. Our study will facilitate molecular research on insecticide resistance in S. avenae, as well as in other wheat aphids.