P0 chr6 sequencing
Whole genome deterministic molecular haplotyping
Included in this data set is the shotgun sequencing of amplified products from three isolated copies of chromosome 6 of P0 (patient zero), whose genome has been previously sequenced using Helicos technology (SRA009216). Each single copy of chromosome 6 was subjected to multiple strand displacement technology and converted into Illumina sequencing library  (labled 'library 1', 'library 2', 'or 'library 3') and was run on two lanes of a flow cell on Illumina Genome Analyzer II.
Conventional experimental methods of studying the human genome are limited by the inability to independently study each of the homologous copies of the chromosomes. These haplotypes are important features of the genome but in general cannot be easily determined. We developed a microfluidic device that is capable of separating and amplifying homologous copies of each chromosome within a single human metaphase  cell. SNP array analysis of amplified materials obtained from single cells enabled us to achieve completely deterministic whole-genome personal haplotypes of four individuals.