ena-STUDY-Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University-21-11-2017-06:18:17:800-1127
Genomic and in situ analyses reveal the Micropruina spp. as abundant fermentative glycogen accumulating organism in enhanced biological phosphorus removal systems
Enhanced biological phosphorus removal (EBPR) configurations, for the treatment of wastewater, involve the cycling of the biomass through carbon-rich (feast) and carbon-deficient (famine) conditions promoting the activity of polyphosphate accumulating organisms (PAO). However, several alternate metabolic strategies, without polyphosphate storage, are possessed by other organisms to compete with the PAO for carbon at the potential expense of EBPR efficiency. The most studied are the glycogen accumulating organisms (GAO), which utilise aerobically stored glycogen to energise anaerobic substrate uptake and storage. In a recent 16S rRNA amplicon survey of full-scale EBPR plants in Denmark, Micropruina spp. were identified as the most abundant of the previously proposed GAO, yet little is known about their ecophysiology. In the current study, genomic and metabolomic studies were made of Micropruina glycogenica str. Lg2T and compared to the in situ physiology of the genus in EBPR plants using state-of-the-art single cell techniques.
Enhanced biological phosphorus removal (EBPR) configurations, for the treatment of wastewater, involve the cycling of the biomass through carbon-rich (feast) and carbon-deficient (famine) conditions promoting the activity of polyphosphate accumulating organisms (PAO). However, several alternate metabolic strategies, without polyphosphate storage, are possessed by other organisms to compete with the PAO for carbon at the potential expense of EBPR efficiency. The most studied are the glycogen accumulating organisms (GAO), which utilise aerobically stored glycogen to energise anaerobic substrate uptake and storage. In a recent 16S rRNA amplicon survey of full-scale EBPR plants in Denmark, Micropruina spp. were identified as the most abundant of the previously proposed GAO, yet little is known about their ecophysiology. In the current study, genomic and metabolomic studies were made of Micropruina glycogenica str. Lg2T and compared to the in situ physiology of the genus in EBPR plants using state-of-the-art single cell techniques.