ena-STUDY-NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY-07-09-2017-13:55:19:334-496
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Here we have used SSH (Suppression Subtractive Hybridization) approach and generated heat stress responsive ESTs in Indian bread wheat cv. C306 by giving heat stress at two different temperatures. pGEM-T vector was used for cloning and sequenced using T7 primer. The generated data was vecsreen to remove vector and adaptor related contamination.
Here we have used SSH (Suppression Subtractive Hybridization) approach and generated heat stress responsive ESTs in Indian bread wheat cv. C306 by giving heat stress at two different temperatures. pGEM-T vector was used for cloning and sequenced using T7 primer. The generated data was vecsreen to remove vector and adaptor related contamination.