GSE161977
Transcriptional profiling of mouse Th17 cells treated with bacterial metabolites 3-oxo-lithocholic acid (3-oxoLCA) and isolithocholic acid (isoLCA)
We report transcriptional profiling of mouse Th17 cells activated and expanded in vitro in the presence of Th17-inhibitory bacterial metabolites 3-oxoLCA and isoLCA. Overall design: Naïve CD4 T cells  from C57BL/6J mice were activated and expanded in Th17 polarization condition for 3 days in vitro. 3-OxoLCA and isoLCA were added 18 hours after T cell receptor (TCR) activation by anti-CD3 and anti-CD28 antibodies.