GSE124027
Genome-wide maps of DNA methylation and gene expression for  multiple  regions  within  a  breast tumor [RRBS]
As the differentiation state of the cells is affected by epigenetic modification regulation, the abnormal epigenetic modification is an important factor leading to the intratumoral heterogeneity. To further understand of the intratumoral epigenetic heterogeneity, we performed Reduced Representation Bisulfite Sequencing and RNA sequencing for multiple regions within a breast tumor and exhibited the epigenetic maps of different regions. We detected intratumoral heterogeneity-related molecular features and further explored the correlation between tumor heterogeneity and tumor hypoxic microenvironment. Overall design: Our  tumor  samples  were  derived  from  a  patient  with  HER2  breast  invasive  ductal carcinoma  at  stage  II.  The  size  of  tumor  was  45  mm×22  mm  and  the  patient  was without  prior  neoadjuvant  therapy.  For  RNA  and  DNA  libraries  preparation,  the tissues  of  tumor  center,  12  o'clock,  3  o'clock,  6  o'clock  and  9  o'clock  of  tumor periphery as well as adjacent of the tumor were taken for 0.6 mg, respectively. Another case of normal breast tissue was used as a control. Totally, 7 samples were examined by RRBS and RNA sequencing.