GSE171585
Natural Variation in the Fitness Consequences of Gene Amplification in Wild Saccharomyces cerevisiae Isolates [RNA-seq]
We used RNA-Seq to measure transcript abundance in 15 Saccharomyces cerevisiae strains from a diverse range of genetic lineages when growing in rich media (YPD) to characterize differential expression across strains. Overall design: RNA-seq and transcriptome analysis of BY4743 and 14 wild S. cerevisiae isolates. Three biological replicate cell samples (collected on different days) were harvested for RNASeq analysis. Cells were grown in YPD for at least three generations to log phase (OD600 ~0.5) and collected by centrifugation.