PRJNA411926
Bacterial 16S ribosomal RNA gene survey from Bothriocroton undatum (goanna tick)
We have collected Australian endemic ticks (Bothriocroton undatum), isolated DNA from equal tick halves using two commercial DNA extractions and sequenced V1-V3 and V3-V4 hypervariable 16S rRNA gene regions. We aimed to assess which combination of DNA extraction and 16S rRNA hypervariable region gives the greatest bacterial diversity, the least amount of bias, and the greatest capacity for identification of Borrelia spp.