ena-STUDY-MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY-04-12-2014-12:36:52:278-256
Mapping of blond of the guppy using ddRADseq
The spontaneous mutant blond of the guppy, Poecilia reticulata, was mapped using ddRADseq in combination with QTL mapping. We used a mapping cross comprised of 134 F2 individuals to identify a candidate region for blond, which contained three candidate genes. All blond fish were homozygous for a 2-bp deletion in adenylate cyclase 5 (adcy5), which is predicted to cause a splicing defect of intron 1 and a premature stop codon. Blond therefore corresponds to the guppy orthologue of adcy5.
The spontaneous mutant blond of the guppy, Poecilia reticulata, was mapped using ddRADseq in combination with QTL mapping. We used a mapping cross comprised of 134 F2 individuals to identify a candidate region for blond, which contained three candidate genes. All blond fish were homozygous for a 2-bp deletion in adenylate cyclase 5 (adcy5), which is predicted to cause a splicing defect of intron 1 and a premature stop codon. Blond therefore corresponds to the guppy orthologue of adcy5.