ena-STUDY-Museu Nacional do Rio de Janeiro-25-10-2017-12:34:04:206-560
THE USE OF NEXT GENERATION SEQUENCING TO SOLVE PHYLOGENETIC INCONGRUENCES IN ORCHIDACEAE
We used the almost complete chloroplast genome of 48 individuals, which are form a clade within the genus. The method consisted in construct genomic libraries, hybridize it with RNA probes designed from available sequences of a related species, and subsequent sequencing of the product. Altogether, we analyzed 131,214 bp, between coding and non-coding regions of the chloroplast genome. The phylogeny was able to resolve the relationship between the selected clade with high support values.
We used the almost complete chloroplast genome of 48 individuals, which are form a clade within the genus. The method consisted in construct genomic libraries, hybridize it with RNA probes designed from available sequences of a related species, and subsequent sequencing of the product. Altogether, we analyzed 131,214 bp, between coding and non-coding regions of the chloroplast genome. The phylogeny was able to resolve the relationship between the selected clade with high support values.