ena-STUDY-TECHNION-ISRAEL INSTITUTE OF TECHNOLOGY-03-04-2021-17:20:20:138-776
Transcriptome profiling of TE03 (I3) human embryonic stem cells (hESCs) by paired-end RNA-Sequencing
Here, we profiled the transcriptome of undifferentiated TE03 (I3) human embryonic stem cells (hESCs) by RNA-sequencing in four independent biological replicates. Libraries were prepared with the Illumina TruSeq library preparation kit v2, followed by sequencing on an Illumina HiSeq 2500 instrument, generating 2 x 101-bp paired-end reads. This data accompanies the manuscript: "Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells". Abstract: "Embryonic stem cell (ESC) self-renewal and cell-fate decisions are driven by a broad array of molecular signals. While transcriptional regulators have been extensively studied in human ESCs (hESCs), the extent to which RNA-binding proteins (RBPs) contribute to human pluripotency remains unclear. Here, we carry out a proteome-wide screen and identify 810 proteins that bind RNA in hESCs. We reveal that many RBPs are preferentially expressed in hESCs and dynamically regulated during early stem cell differentiation. Notably, nearly 200 RBPs are affected by knockdown of OCT4, a master regulator of pluripotency, several dozen of which are directly bound by this factor. Using cross-linking and immunoprecipitation (CLIP-Seq), we discover that the pluripotency-associated STAT3 and OCT4 transcription factors interact with RNA in hESCs and confirm the binding of STAT3 to the conserved NORAD long-noncoding RNA. Taken together, our findings indicate that RBPs have a more widespread role in human pluripotency than previously appreciated".
Here, we profiled the transcriptome of undifferentiated TE03 (I3) human embryonic stem cells (hESCs) by RNA-sequencing in four independent biological replicates. Libraries were prepared with the Illumina TruSeq library preparation kit v2, followed by sequencing on an Illumina HiSeq 2500 instrument, generating 2 x 101-bp paired-end reads. This data accompanies the manuscript: "Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells". Abstract: "Embryonic stem cell (ESC) self-renewal and cell-fate decisions are driven by a broad array of molecular signals. While transcriptional regulators have been extensively studied in human ESCs (hESCs), the extent to which RNA-binding proteins (RBPs) contribute to human pluripotency remains unclear. Here, we carry out a proteome-wide screen and identify 810 proteins that bind RNA in hESCs. We reveal that many RBPs are preferentially expressed in hESCs and dynamically regulated during early stem cell differentiation. Notably, nearly 200 RBPs are affected by knockdown of OCT4, a master regulator of pluripotency, several dozen of which are directly bound by this factor. Using cross-linking and immunoprecipitation (CLIP-Seq), we discover that the pluripotency-associated STAT3 and OCT4 transcription factors interact with RNA in hESCs and confirm the binding of STAT3 to the conserved NORAD long-noncoding RNA. Taken together, our findings indicate that RBPs have a more widespread role in human pluripotency than previously appreciated".