E-MTAB-10861
RNA-seq of human breast cancer cell line T47D and 2 different T47D_H2A.J-KO cells lines treated with tamoxifen and untreated controls
The experiment is intended to test the effect of the Cripsr/Cas9 deletion of the H2AFJ gene encoding the histone variant H2A.J on the transcriptome of luminal breast cancer T47D cells in conditions of proliferation, and after proliferative arrest induced by treating the cells with 5 µm tamoxifen for 4 days. Two independent H2A.J-KO cell lines were analyzed: KO1 and KO18.
The experiment is intended to test the effect of the Cripsr/Cas9 deletion of the H2AFJ gene encoding the histone variant H2A.J on the transcriptome of luminal breast cancer T47D cells in conditions of proliferation, and after proliferative arrest induced by treating the cells with 5 µm tamoxifen for 4 days. Two independent H2A.J-KO cell lines were analyzed: KO1 and KO18.