97cd1bb5-b14c-4810-85d6-0c62ef395ee8
Raw metagenomic sequences from water samples collected around an Acropora pulchra colony (Tema'e Beach reef, Mo'orea)
In this work, samples for DNA collection from waters around an Acropora pulchra colony were taken in April 25th, 2018 (12:00 local time) at the Tema'e beach reef of Mo'orea (French Polynesia). Water samples were withdrawn from two different local sites around the colony, at a depth of ~1 m: (IN) between the living branches, ~1 cm next to the polyps; and (OUT) 2 meters down current from the patch over a sandy bottom ~2 m deep. At that sampling point, 0.8 L (IN) and 2.5 L (OUT) seawater were sampled and were prefiltered through 200-µm mesh. The microbial biomass was then collected on 0.2 µm pore-size, 47 mm diameter polycarbonate filters using a peristaltic pump. The filters were flash frozen in liquid N2 and stored at -80°C. Total DNA was extracted using the phenol-chloroform protocol as described in Massana et al. (1997). Whole metagenome sequencing of DNA extracts was performed using a PCR free protocol at the Centre Nacional d'Anàlisi Genòmica (CNAG, Barcelona, Spain; http://cnag.cat/). Short-insert paired-end libraries were prepared with the Illumina TruSeq Sample Preparation kit (Illumina Inc.) and sequenced on a NovaSeq 6000 Illumina platform (2 x 150 bp), yielding about 45 Gb of sequencing information per metagenome.   References:  Massana, R., Murray, A. E., Preston, C. M., and DeLong, E. 1302 F. (1997). Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Appl. Environ. Microbiol. 63, 50–56.
In this work, samples for DNA collection from waters around an Acropora pulchra colony were taken in April 25th, 2018 (12:00 local time) at the Tema'e beach reef of Mo'orea (French Polynesia). Water samples were withdrawn from two different local sites around the colony, at a depth of ~1 m: (IN) between the living branches, ~1 cm next to the polyps; and (OUT) 2 meters down current from the patch over a sandy bottom ~2 m deep. At that sampling point, 0.8 L (IN) and 2.5 L (OUT) seawater were sampled and were prefiltered through 200-µm mesh. The microbial biomass was then collected on 0.2 µm pore-size, 47 mm diameter polycarbonate filters using a peristaltic pump. The filters were flash frozen in liquid N2 and stored at -80°C. Total DNA was extracted using the phenol-chloroform protocol as described in Massana et al. (1997). Whole metagenome sequencing of DNA extracts was performed using a PCR free protocol at the Centre Nacional d'Anàlisi Genòmica (CNAG, Barcelona, Spain; http://cnag.cat/). Short-insert paired-end libraries were prepared with the Illumina TruSeq Sample Preparation kit (Illumina Inc.) and sequenced on a NovaSeq 6000 Illumina platform (2 x 150 bp), yielding about 45 Gb of sequencing information per metagenome.   References:  Massana, R., Murray, A. E., Preston, C. M., and DeLong, E. 1302 F. (1997). Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Appl. Environ. Microbiol. 63, 50–56.