PRJNA379998
small RNA sequencing of EMT cells and released EVs
Epithelial mesenchymal transition (EMT) is characterized by a shift in cellular plasticity whereby epithelial cells acquire mesenchymal traits that include spindle-shaped morphology, and increased cell migration and invasion. EMT is thought to promote various stages of the metastatic cascade; a process governing passage of primary tumour cells to a distant site for colonization and secondary tumour growth. In the tumour microenvironment (TM), extracellular proteases exert pleiotropic effects that include EMT regulation, invasion, angiogenesis, growth factor signalling and extracellular matrix (ECM) remodeling. Collectively, cancer-associated proteases enhance metastatic progression, however, not all the molecular mechanisms have been defined, including many protease-substrate interactions. In this study, we profiled small RNAs in epithelial Madin-Darby canine kidney (MDCK) cells with oncogenic H-Ras (21D1 cells) and released EVs, as well as in the cells and EVs of MDCK cells with over-expression of YBX1 (MDCK+YBX1) and 21D1 cells with knocked-down MMP1 expression (21D1-MMP1).