GSE173521
Tbx1 binding site in whole mouse embryo at E9.5 from in vivo ChIP-seq
We generated Avidin tag inserted at 3' end of Tbx1 using CRISPR/Cas9 genomic engineering and crossed them with BirA mice from the Jackson laboratory. After established double homozygous mouse colony, total twenty embryos at E9.5 were collected for each samples. After extraction of nucleus, Tbx1 binding regions were captured with Dynabeads™ MyOne™ Streptavidin T1. Overall design: Tbx1 is T-box transcription factor which expresses in pharyngeal apparatus in mouse embryo. To capture Tbx1 binding site in vivo,  nucleus from Tbx1-Avi;BirA double homozygous embryos at E9.5  were collected for ChIP-assay with streptavidin beads