ena-STUDY-CBMSO-04-04-2017-10:39:08:689-498
In this project, we have sequenced again the genome of Leishmania infantum JPCM5 strain by the use of two NGS technologies: Illumina sequencing and single-molecule, real-time sequencing (Pacific Biosciences, PacBio). Note that Illumina sequencing library was divided into two sequencing lines, and two pairs of paired-reads will be provided for the same sample. (SAMEA103958196 and SAMEA103958197).
Leishmania infantum (Nicolle, 1908) was named after its identification as the causative agent of visceral leishmaniasis in the Mediterranean basin. This species is also found in Latin America, where it was named Leishmania chagasi. Apart from humans, canids are also susceptible hosts and often result affected by the pathologies caused by the infection with this Leishmania species.The L. infantum JPCM5 strain (MCAN/ES/98/LLM-877) was selected to determine the genome sequence of a viscerotropic species of the genus Leishmania, and the results were published in 2007 (ENA genome assembly: ASM287v2). A whole-genome shotgun strategy was used for generating a genomic library, and individual clones were sequencing following classical Sanger sequencing. The resulting assembly contained 470 contigs.In this project, we have sequenced again the genome of this strain by the use of two NGS technologies: Illumina sequencing and single-molecule, real-time sequencing (Pacific Biosciences, PacBio). Combination of both sequencing data has led to the generation of a robust assembly for the complete genome, consisting in 36 contigs, which account for the 36 chromosomes having this Leishmania species.
Leishmania infantum (Nicolle, 1908) was named after its identification as the causative agent of visceral leishmaniasis in the Mediterranean basin. This species is also found in Latin America, where it was named Leishmania chagasi. Apart from humans, canids are also susceptible hosts and often result affected by the pathologies caused by the infection with this Leishmania species.The L. infantum JPCM5 strain (MCAN/ES/98/LLM-877) was selected to determine the genome sequence of a viscerotropic species of the genus Leishmania, and the results were published in 2007 (ENA genome assembly: ASM287v2). A whole-genome shotgun strategy was used for generating a genomic library, and individual clones were sequencing following classical Sanger sequencing. The resulting assembly contained 470 contigs.In this project, we have sequenced again the genome of this strain by the use of two NGS technologies: Illumina sequencing and single-molecule, real-time sequencing (Pacific Biosciences, PacBio). Combination of both sequencing data has led to the generation of a robust assembly for the complete genome, consisting in 36 contigs, which account for the 36 chromosomes having this Leishmania species.