ena-STUDY-INRAE-17-12-2019-08:47:37:858-1241
Impact of prepubertal nutritional modulation on bull sperm DNA methylation
Our objective was to evaluate the methylation changes in sperm of bulls exposed to different pre-pubertal diets.  One-week-old Holstein bull calves (n=9), randomly allocated to 3 groups, were fed either a high, medium or low-diet (20%, 17% or 12.2% Crude protein and 67.9%, 66% or 62.9% Total digestible nutrients, respectively) from 2 to 32 weeks of age, followed by medium-nutrition. Semen collected from bulls at two specific time points; 55-59 and 69-71 weeks was diluted, cryopreserved and used for reduced representation bisulfite sequencing. Differential methylation was detected for dietary treatment, but minimal differences were detected with age.
Our objective was to evaluate the methylation changes in sperm of bulls exposed to different pre-pubertal diets.  One-week-old Holstein bull calves (n=9), randomly allocated to 3 groups, were fed either a high, medium or low-diet (20%, 17% or 12.2% Crude protein and 67.9%, 66% or 62.9% Total digestible nutrients, respectively) from 2 to 32 weeks of age, followed by medium-nutrition. Semen collected from bulls at two specific time points; 55-59 and 69-71 weeks was diluted, cryopreserved and used for reduced representation bisulfite sequencing. Differential methylation was detected for dietary treatment, but minimal differences were detected with age.