GSE134059
UPF1 regulatory loop variant RNA-Seq and RIP-Seq
HEK-293 Trex cells containing stable tetracycline-inducible transgenes driving overexpression of CLIP-tagged UPF1 isoforms 1 and 2 or GFP were used for total RNA-seq. In addition, CLIP-UPF1 isoforms were affinity purified, and bound RNA was subjected to high-throughput sequencing. Overall design: Paired-end high-throughput RNA sequencing of Total RNA from cells expressing CLIP-tagged UPF1 isoform 1, UPF1 isoform 2, or GFP and RNA associated with affinity-purified CLIP UPF1 isoforms 1 and 2.