GSE114049
Next Generation Sequencing to determine the fundamental mechanisms of Tribolium castaneum larvae to cope with hypoxia
We used high-throughput sequencing technology to determine the fundamental mechanisms of Tribolium castaneum to cope with hypoxia. Totals of 12,524,554 useful reads from the control group and 14,193,339 useful reads from the treatment group were generated after Q20 filtering. A total of 16,524 unigenes had been annotated. More than 300 transcripts showed differential expression between the control and hypoxia group, with a fold change =1.5 and p value <0.05. Of the 333 identified DEGs, 154 were downregulated and 179 were upregulated. Altered expression of 9 genes was confirmed with qRT–PCR, demonstrating the qRT-PCR data matched the RNA-seq results well. Finally, our results indicated that limiting the available oxygen to T. castaneum increased glycolysis and inhibited the Krebs cycle, and that accumulated pyruvic acid was preferentially converted to lactic acid via anaerobic metabolism. This study advances the field of pest control and makes it possible to develop more efficient strategies for hermetic storage. Overall design: Examination of gene expression in Tribolium castaneum larvae after 12 h hypoxia treatment, compared with normoxic ones