PRJNA663917
Targeted sequencing of hypermutable and conserved regions of the SARS-CoV-2 genome
SARS-CoV-2 has spread rapidly around the world and affected diverse populations. In the course of its spread, the virus acquired genetic variation leading to species-level mutations as well as quasispecies that are unique to an individual. This study sequenced viral RNA from clinical nasopharyngeal swab samples and contrived samples made by spiking commercially available SARS-CoV-2 isolates from Wuhan, Hong Kong, and Washington state into human saliva samples. The samples were sequenced with primers designed to target both hypermutable and conserved genomic regions. A primer for the human gene RPP30 was designed as a positive control.