GSE204777
Space-time Mapping Identifies Concerted Multicellular Patterns and Gene Programs in Healing Wounds and their Conservation in Cancers.
Tissue repair responses in metazoans are highly coordinated by different cell types over space and time. However, comprehensive single-cell based characterization covering this coordination is lacking. Here, we captured transcriptional states of single cells over space and time during skin wound closure, revealing choreographed gene expression profiles. We identified shared and prominent space-time patterns of cellular and gene expression enrichment: which we call multicellular 'movements' and which spanned multiple cell types.  We validated some of the discovered space-time movements using large volume imaging of cleared wounds and demonstrated the value of this analysis to predict gene products made by macrophages or fibroblasts, which activated gene programs in the opposite cell type. Finally, using two different tumor models, we tested the hypothesis that tumors are like 'wounds that never heal' finding conserved wound healing movements in the tumor space, wherein some movements were preferentially used in one tumor versus another. Overall design: Single cell RNA-Seq data of sorted immune and non-immune cells from unwounded mouse skin, and wounds d1,3,7,14 post wounding from 4 pooled wounds. Samples multiplexed based on time and radial position in wound