E-MTAB-10550
ChIP-seq mapping of gH2AX, PanKcr, and H3K9cr at AsiSI-induced double-strand breaks in control and CDYL1-depleted U2OS-DIvA cells
Control and CDYL1-depleted U2OS-DIvA cells were treated with 4-hydroxy tamoxifen (4OHT) to induce multiple DNA double-strand breaks (DSBs) at defined loci in the genome via AsiSI restriction enzyme. This system was used to map the changes in lysine crotonylation (Kcr) at DSB sites in control and CDYL1-deficient cells.
Control and CDYL1-depleted U2OS-DIvA cells were treated with 4-hydroxy tamoxifen (4OHT) to induce multiple DNA double-strand breaks (DSBs) at defined loci in the genome via AsiSI restriction enzyme. This system was used to map the changes in lysine crotonylation (Kcr) at DSB sites in control and CDYL1-deficient cells.