GSE200820
Calorie restriction and calorie dilution have different impacts on body fat, metabolism, behavior and hypothalamic gene expression
Caloric restriction is a robust intervention to increase life span. Giving less food (CR), or allowing free access to diluted diet with indigestible components (CD) are two methods to impose restriction. CD does not generate the same lifespan effect as CR. We compared responses of C57BL/6 mice to equivalent levels of CR and CD. Our study calorie restriction and calorie two restriction methods different effect on hypothamic gene expression pattern, RNA-seq was performed using hypothalamus of mice with 2 methods treatment as well as control group. Overall design: There are three groups in total,control group (CT), calorie restriction group (CR) and dietary dilution group (CD) were exposed to the corresponding experimental diets for 3 months. All animals used in this study were C57BL/6J male mice that were purchased at age 8 weeks from Charles River Laboratories. The mice were single housed in a specific pathogen free facility at 60% humidity with a 12-hour light /12-hour dark circle (light, 7:30 am to 7:30 pm). All the mice were under ad libitum access to water and standard diet (D12450B) in the first 30 days as the baseline period.Afterwards, mice were divided into 3 groups - control group (CT) (D12450B diet), calorie restriction group (CR) and dietary dilution group (CD) were exposed to the corresponding experimental diets for 3 months. CR mice were pair fed the baseline diet to the absorbed intake of ad lib fed CD mice fed the high cellulose diet. The energy intake of CD group was calculated daily by weighing the food that went missing from the hopper (plus any food items recovered from the bedding). This was calculated for each mouse. We then provided paired mice from the CR group with the corresponding amount of standard diet  adjusted for the absorption efficiency and dilutent. The food was provided just before lights off on that day in the evening. CT mice were under ad libitum supply of the baseline diet throughout. In the end of the experiment, mice were sacrificed and hypothalamus part were collected for RNA extraction and sequencing.