Gene_expression_changes_upon_dietary_restriction_switch_in_mice-sc-2013-09-20T11:32:57Z-2773
Gene_expression_changes_upon_dietary_restriction_switch_in_mice
RNA was extracted from mice and mRNA isolated from 2ug total RNA. mRNA was processed to generate a library compatible with Illumina high throughput sequencing using a standard RNA-seq library generation protocol. Each library was amplified using indexed primers and samples were purified using AMPure XP beads. Libraries were pooled and quantified with Bioanalyzer and qPCR.   This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Dietary restriction (DR) extends lifespan in a broad range of organisms including mammals. In order to determine whether dietary restriction (DR) has acute and reversible effects on mortality in mammals, we conducted a late-onset DR switch experiment using mice. Therefore, old mice were switched from ad libitum (AL) feeding to DR and from DR to AL feeding and mortality rates were determined. In order to measure gene expression changes associated with the DR switch we collected several tissues 2 month after the switch. The goal of this study is to identify diferentially regulated genes between DR and AL conditions, which underlay the beneficial effects of DR on lifespan.