GSE201174
Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies
We generate macrophages from pluripotent stem cells (PSCs) with GD2 CARs integrated into AAVS1 locus. To produce CAR macrophages (CAR-M) we established a serum- and feeder-free differentiation protocol which generates macrophages through arterialized hemogenic endothelium. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CAR_M and WT_M cells with and without CHLA co-culture