ena-STUDY-Institute of oral biology-01-04-2019-09:19:50:635-212
cDNA from ten different strains of P.gingivalis
Ten P. gingivalis strains were incubated in BBL broth with hemin , vitK and 10% human blood. Bacteria samples were collected after 3, 5, 7, 8 and 11 days directly into RNA later.Total RNA was extracted, rRNA, tRNA and DNA remnant were removed and tested by Bioanalyzer agilent. Then, mRNA was converted to cDNA and Illumina library prep was performed by Nextera XT including index labeling.
Ten P. gingivalis strains were incubated in BBL broth with hemin , vitK and 10% human blood. Bacteria samples were collected after 3, 5, 7, 8 and 11 days directly into RNA later.Total RNA was extracted, rRNA, tRNA and DNA remnant were removed and tested by Bioanalyzer agilent. Then, mRNA was converted to cDNA and Illumina library prep was performed by Nextera XT including index labeling.