DRP006648
Metatranscriptomic sequencing of synthetic microbiota (six and three)
The two co-culture experiments were carried out using 3 species and 6 species, respectively. The strains were activated in sorghum extract medium at 30 ?C for 24 h. The co-culture experiments were carried out in 2 L fermentation tanks using the same medium, and at 30 ?C for 48 h, including three parallel experiments. The initial concentration of each specie was 1 ? 106 CFU/mL. The fermented broth at 24h was stored at -80 ?C until analysis.