DRP006710
Genome-wide analysis of mRNA cleavage sites in in Arabidopsis thaliana using expanding leaf
In a previous study, a high-throughput approach for genome-wide profiling of RNA cleavage sites was developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3' transcripts; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) in Arabidopsis thaliana (expanding leaf, three newer leaves were collected from 21days after germination) to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.