GSE130448
Mucociliary epidermis RNA-seq data from comparison of controls and DN-tp63 morpholino knockdown in Xenopus animal cap explants.
This study was conducted to investigate the effects of DN-tp63 loss-of-function on mucociliary basal stem cells in Xenopus laevis. Uninjected control embryos and DN-tp63 morpholino oligonucleotide injected embryos were used to generate animal cap explants at embryonic stage 8. Explants were grown into mucociliary organoids and collected at embryonic stages 10.5, 16-19, and 24-25 for total RNA extraction. Overall design: The experiment was conducted in three biological replicates (embryos were derived from three different females and fertilizations). Control samples remained uninjected, DN-tp63 morphant samples were injected with morpholino oligonucleotides targeting the start condon of DN-tp63 and resulted in protein knockdown (loss-of-function). Sequencing was performed in two seperate paired-end runs to increase sequencing dpth, with each run containing all samples.