PRJNA718418
Wetland soils nitrogen cycle microbes
16S, SSU and ITS amplicons of bacteria, archaea and fungi (+other eukaryotes).*For bacteria, primers 515F (GTGYCAGCMGCCGCGGTAA) and 806RB (GGACTACNVGGGTWTCTAAT)* For archaea, primers SSU1ArF (TCCGGTTGATCCYGCBRG) and SSU1000ArR (GGCCATGCAMYWCCTCTC).*For eukaryotes, primers ITS9mun (GTACACACCGCCCGTCG) and ITS4ngsUni (CGCCTSCSCTTANTDATATGC) that cover the V9 variable region of the rRNA 18S gene and the full internal transcribed spacer (ITS) region.