Transcriptome_profiling_of_mutant_embryos_from_the_Mouse_Genetics_Project__DMDD_-sc-3040
Transcriptome_profiling_of_mutant_embryos_from_the_Mouse_Genetics_Project__DMDD_
Protocol: Total RNA was extracted from mouse embryos and DNase treated. Fragmented RNA was enriched for the 3’ ends by pull down using an anchored polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 1 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 2 at the 5’ end followed by 10 bases HBDVHBDVHB (using the single base code), then one of 96 eight base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 20 cycles of PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Paired-end sequence data from Illumina HiSeq was prepared from mouse embryos from the Mouse Genetics Project for transcriptome profiling