GSE136166
Genome-wide identification of WblC (WhiB7) binding sites in Streptomyces coelicolor A3(2) treated with tetracycline
We determined Streptomyces coelicolor genes that are directly regulated by WblC (or WhiB7), an actinobacterial transcription factor that activates expression of intrinsic resistance in response to translation-inhibitory antibiotic stress. Identification of differentially expressed genes in wblC mutant by RNA-seq and WblC binding sites in wild type by ChIP-seq identified more than 300 genes as WblC regulon. This series encompasses the ChIP-seq data of our study. Overall design: Chromosomal DNA-binding peaks of WblC were determined from tetracycline-treated wild type using Illumina HiSeq. Chromatin immunoprecipitation (ChIP) and input DNA samples were prepared by pooling biologically independent triplicates, respectively.