GSE137945
Next Generation Sequencing of PKD2 mutant mice
Purpose: Determine the differential gene expression pattern between wildtype, Pkd2-KO and Pkd2-miR-214 KO mice Methods: kidney mRNA profiles of Pkd2-KO and Pkd2-mir-214-KO mice was sequenced with N of 3 in each group Results: 972 differentially expressed transcripts were identified between Pkd2-KO kidneys and Pkd2-miR-214-KO kidneys Conclusion: Deletion of miR-214 promotes interstitial inflammation in mouse models of ADPKD Overall design: kidney mRNA profiles of Pkd2-KO and Pkd2-miR-214 KO were determined by polyA mRNA sequencing with an N=3 different biological replicates in each group