ena-STUDY-CNRS - Universite Lyon 1-28-11-2018-09:16:14:173-1299
Analysis of the distribution of meiotic recombination events in Paramecium tetraurelia.
To analyze the distribution of meiotic recombination events in Paramecium tetraurelia, we performed crosses between two strains (strain 32 x strain 51). The level of heterozygosity in the resulting F1's is about 0.3%. We then induced autogamy to obtain F2's. We extracted and sequenced DNA (essentially MAC and mitochondrial DNA) from  42 F2's.
To analyze the distribution of meiotic recombination events in Paramecium tetraurelia, we performed crosses between two strains (strain 32 x strain 51). The level of heterozygosity in the resulting F1's is about 0.3%. We then induced autogamy to obtain F2's. We extracted and sequenced DNA (essentially MAC and mitochondrial DNA) from  42 F2's.