GSE202669
Deletion of centrally conserved domain intergenic to H19 and Igf2 perturbs adult skeletal muscle specific Igf2 expression
Intergenic to H19 and Igf2, there is a previously identified centrally conserved domain (ccd ) that includes two sequences with high conservation between human and mouse. At one of these sequence elements there is DNAse1 Hypersentitivity sequence (HSS), CTCF binding and chromatic interaction/looping to sequence upstream of Igf2 on both parental alleles. In earlier studies, removal of ccd sequence from the maternal allele resulted in activation of the normally repressed maternal Igf2 in neonatal limb. We have generated an independent ccd (floxed) deletion allele. Deletion of ccd from both the maternal and paternal allele also results in elevated levels of Igf2 expression from the respective alleles in adult skeletal muscle.  Capture C analysis of adult skeletal muscle nuclei uncovers new chromatin interactions in ccd deletion alleles. Overall design: Capture-C on F1 hybrid neonatal limb and adult skeletal muscle derived from reciprocal crosses between C57BL/6 (B6) mice heterozygous for described deletion and mice homozygous for the Mus Castaneous on Chromosome 7 strain (C7).