SNP_GENOTYPING_M_ROSENBERGII
Genotyping of Growth Selected Macrobrachium rosenbergii Using SNPs from Expressed Sequences
Eleven M. rosenbergii genes having important physiological roles were selected for this study. Agarwal et al. (2016) had mined SNPs from certain regions of these genes and one fragment was selected from each gene based on the presence of highest number of SNPs. These genes fragment were amplified using Sanger sequencing method from 23 growth selected individuals from ICAR-CIFA, Odisha (Lat: 20° 10' 59.358'' N, Long: 85° 51' 5.1948'' E) and 23 wild individuals from Vembanad Lake, Kerala (Lat: 9° 34' 59.9988'' N, Long: 76° 25' 0.0012'' E) . A total of 45 SNPs were found from these gene fragments.
Eleven M. rosenbergii genes having important physiological roles were selected for this study. Agarwal et al. (2016) had mined SNPs from certain regions of these genes and one fragment was selected from each gene based on the presence of highest number of SNPs. These genes fragment were amplified using Sanger sequencing method from 23 growth selected individuals from ICAR-CIFA, Odisha (Lat: 20° 10' 59.358'' N, Long: 85° 51' 5.1948'' E) and 23 wild individuals from Vembanad Lake, Kerala (Lat: 9° 34' 59.9988'' N, Long: 76° 25' 0.0012'' E) . A total of 45 SNPs were found from these gene fragments.