GSE96958
Single-cell RNAseq analysis of the i8TF cell lines with/without exposure to dox
The aim of the experiment was to assess the cell heterogeneity after the doxycycline treatment and the subsequent induction of the 8 transcription factors. Overall design: Cells were cultured in BL-CFC culture for 1 day before sorting for eVSM cells. Then, sorted eVSM were cultured in hemogenic endothelium medium for 24h with or without doxycycline. Cells from the two conditions were then mixed at a 1:1 ratio.