E-MTAB-7111
RNA-seq of mouse myeloma cell line SP 2/0 cells overexpressed with LOC108168067 against vector control
We found that a novel gene LOC108168067 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line (ATCC® CRL-1581™, https://www.atcc.org/products/all/CRL-1581.aspx) was selected to test the effect of LOC108168067 overexpression on plasmablast/plasma cells. LOC108168067 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV201 (Fugene Corp., Guangzhou, China) to generate LOC108168067 protein. LOC108168067-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 µg/ml). To explore the effect of on gene expression, we determined mRNA profiles in LOC108168067-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.
We found that a novel gene LOC108168067 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line (ATCC® CRL-1581™, https://www.atcc.org/products/all/CRL-1581.aspx) was selected to test the effect of LOC108168067 overexpression on plasmablast/plasma cells. LOC108168067 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV201 (Fugene Corp., Guangzhou, China) to generate LOC108168067 protein. LOC108168067-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 µg/ml). To explore the effect of on gene expression, we determined mRNA profiles in LOC108168067-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.