PRJNA264430
Homo sapiens strain:HEK293T Targeted Locus (Loci)
To evaluate the DNA cleavage specificity of Cas9 delivered via various methods, we measured the modification of Cas9 on-target and off-target sites of CLTA, EMX, and VEGF genomic target sites in human tissue culture. The target site and its corresponding known off-target sites were amplified from genomic DNA isolated from HEK293 cells treated with Cas9 and one sgRNA targeting the CLTA site, sgRNA targeting the EMX site, one sgRNA targeting the VEGF site.