E-MTAB-10484
RNA-seq to investigate circadian regulation of gene expression in Medicago truncatula Jester accession.
The experiment looks for circadian-regulated genes in Medicago truncatula plants. Medicago truncatula Jester accession plants were entrained for 3 weeks in long days (16h light:8h dark) at 24°C constant temperature. On day 20, plants were transferred to LL conditions for 3 days. Samples for three biological replicates per time point were collected every 4h for 24h beginning at CT48 (subjective dawn) until CT68. Total RNA was extracted from ground tissue using an RNeasy RNA extraction kit (Qiagen). Extracted total RNA was DNAse-treated (Invitrogen). Total RNA (2-3ug) was dried down and preserved in Sigma-Aldrich RNAstable before being couriered to BGI Tech Solutions (HONGKONG) Co., Ltd (https://www.bgi.com/global). BGI Tech prepared and processed libraries for 20M PE100 reads using their DNBseq platform - BGISEQ-500.
The experiment looks for circadian-regulated genes in Medicago truncatula plants. Medicago truncatula Jester accession plants were entrained for 3 weeks in long days (16h light:8h dark) at 24°C constant temperature. On day 20, plants were transferred to LL conditions for 3 days. Samples for three biological replicates per time point were collected every 4h for 24h beginning at CT48 (subjective dawn) until CT68. Total RNA was extracted from ground tissue using an RNeasy RNA extraction kit (Qiagen). Extracted total RNA was DNAse-treated (Invitrogen). Total RNA (2-3ug) was dried down and preserved in Sigma-Aldrich RNAstable before being couriered to BGI Tech Solutions (HONGKONG) Co., Ltd (https://www.bgi.com/global). BGI Tech prepared and processed libraries for 20M PE100 reads using their DNBseq platform - BGISEQ-500.