GSE167763
RNA-SEQ OF 24H TIME COURSE OF MOUSE FIBROBLASTS ENTRAINED WITH DIFFERENT CYCLIC METABOLIC  ENTRAINMENTS
In our daily routine we are continually exposed to feeding ang fasting cycles, and it is important to take into consideration the overall coherence between metabolism and circadian rhythms. The loss of such a coherence, translating in a misalignment, may contribute to the weakening or desynchronization of oscillators.   In this study we asked whether the synchronization between the feeding-fasting cycles and the cell-autonomous clock affects the entire transcriptome of mouse fibroblasts in vitro. We found that in the metabolic entrainment proposed in this work and based on sustained 3 days of feeding and fasting cycles (HL, LH, HH), RNA-sequencing data from this work show a significant number of oscillating genes (N=500) belong to the transcriptome in all the dietary regimes. This result could be the consequence of the cyclical nature of the metabolic perturbations implemented in our study.    Moreover, our investigation has also shown that the fasting phase is important to have a phase polarization of the transcriptome, that is completely missing in the HH condition where the cells were always kept in high level of nutrients. It is worth mentioning that the misalignment leads to a polarized expression of ECM protein which is a typical mechanism of stimulated fibroblasts that is frequently associated with fibrosis. It was observed that this polarization involves different pools of genes and as consequence is enriched for different metabolic functions. All these findings confirm that regulation of metabolism by the circadian clock and its components is reciprocal, attesting that metabolism and circadian clocks are tightly interlocked: clocks drive metabolic processes, and various metabolic parameters affect clocks, producing complex feedback relationships. Overall design: Comparison of the different effects of metabolic oscillations  on the transcrioptome entrainment