PRJNA828597
ITS2 Fungi amplicon from Culex quinquefasciatus
Fungal metagenome derived from total DNA of a sample Culex quinquefasciatus reared on laboratory conditions were characterized, ITS2 region was amplified using ITS3/ITS4 primer pair. Groups of 20 individuals of larvae, males, females only fed on sucrose solution and females fed on mouse blood were selected and surface was sterilize. Genomic DNA was extracted with DNeasy Blood &amp; Tissue kit (QIAGEN) according to the manufacturer's protocol for insect tissues.The diversity of fungal communities in different developmental stages, sexes, and adult nutrition of Culex quinquefasciatus reared on laboratory conditions were characterized.