PRJNA869952
Bulk RNA Sequencing of antibiotic-treated SPF, healthy-colonized, and CMA-colonized mice
RNA libraries for total RNA from ileal epithelial cells were prepared and sequenced at the University of Chicago Functional Genomics Core. Libraries were prepared using a TruSeq Stranded Total Library Preparation Kit with Ribo-Zero Human/Mouse/Rat (Illumina). Samples were sequenced using 50-bp single-end reads chemistry on the HiSeq2500 instrument.