PRJNA489308
Over-expression of EVI-1 oncogene regulate multi-pathway to affect zebrafish embryogenesis and hemopoiesis
Our group established Tg (Tol-GFP-EVI-1) zebrafish line and detected survival rate and developmental abnormality in injected embryos. And we found Tg (Tol-GFP-EVI-1) zebrafish embryos underwent abnormal hematopoiesis and aberrant expression of human EVI-1 perturbs hematopoiesis to promote myeloid myeloid and suppress erythroid lineage fates. Moreover, overexpression of human EVI-1 induced immature hematopoietic cells emerged in blood circulation. We used whole genome transcriptome analysis (RNA-Seq) to determine differential gene expression in peripheral blood between wildtype larvae and EVI-1 transgenic zebrafish larvae.