GSE112325
AMPKa2 knockout mice and usage of alternative transcription start sites
Role of alternative transcription start sites in muscle growth and development remains largely undetermined.  Our WTSS-seq (whole transcriptome start site sequencing) approach to capture 5''-end of RNAs clearly revealed alternative transcription start  events responsible for  increased muscle mass in AMPK a2 knockout mice. Overall design: Total RNA samples obtained from muscle tissue of knockout (KO) and wild-type (WT) mice were used to construct WTSS-seq libraries. Four WTSS-seq libraries, including two AMPK a2 KO mice and two AMPK a2 WT mice were involved in this submission.