GSE99958
Next-generation sequencing of PDLSC transcriptome
We performed RNA-sequencing and miRNA-sequencing to detect the whole transcriptome of periodontal ligament stem cells (PDLSCs) at different stages during osteogenic differentiation. Overall design: PDLSCs were isolated and cultured in osteogenic medium. Total RNA from cells at D0, D3, D7, and D14 were extracted, and submitted to RNA-sequencing and miRNA-sequencing.