PRJNA431815
The profile of Arabidopsis small RNAs that are presented in purified B. cinerea protoplasts from the infected tissues, extracellular vesicles and Arabidopsis leaves.
B. cinerea protoplasts were purified from infected 4-week-old Arabidopsis leaves. Plant extracellular vesicles were isolated and purification by differential ultracentrifugation from apoplastic fluids of 4-week-old Arabidopsis leaves. RNAs was extracted using the Trizol method. Small RNA fraction of 18-40 nucleotides was isolated by 14 % 8 M Urea PAGE. The sRNA libraries were made using Illumina TruSeq Small RNA Sample Prep Kits and sequenced by Illumina sRNA-seq system.