DRP006821
Chromatin immunoprecipitation of HEK293T cells using native and crosslinked ChIP methods.
Chromatin immunoprecipitation of HEK293T cells was performed using Fractionation-assisted native chromatin immunoprecipitation method (fanChIP) and Fractionation-assisted crosslinked chromatin immunoprecipitation method (faxChIP) described in Okuda et al. [Journal of Clinical Investigation 127(5) 1918-1931(2017)].Specific antibodies for RNA Polymerase II phosphorylated on Ser 5 in its  heptapeptide repeats [RNAP2 Ser5-P (CTD4H8)]  and its non-phosphorylated form [RNAP2 non-P (8WG16)] were used for IP. The ChIPed DNAs were analyzed by deep sequencing.