GSE97766
Gene Expression Profiling of Gemcitabine, Genistein and Combinatorial Effects on Pancreatic Cancer Cell Lines
We reported the transcriptional effects of gemcitabine, genistein and the combined gemcitaibe/gensitein treatments on two pancreatic cancer cell lines MiaPaCa2 and PANC1. The overall purpose of the study to understand the underlying mechanism of genistein chemoenhancing function. Overall design: Two pancreatic cancer cell lines were treated either with 40nM of gemcitabine (Gem2), 50uM of genistein (Gen) or in combination for 24 hours. RNA was isolated and purified by using Qiagen RNAasy kit. The quality and concentraion of RNA were detected by using bioanalyzer.