TraDIS_Typhimurium_1M
TraDIS_Typhimurium_1M, submitted by SC on 20-MAY-13
An estimated 930,000 transposon mutants in S. Typhimurium have been assayed using Illumina nucleotide sequencing from within the transposon into the adjacent target DNA. Using TraDIS (Transposon Directed Insertion-site Sequencing), we have been able to map ~550,000 unique transposon insertion sites to the Salmonella enterica serovar Typhimurium chromosome. This has allowed us to assay simultaneously every gene in the genome for essentiality and generate a genome-wide list of candidate essential genes.
An estimated 930,000 transposon mutants in S. Typhimurium have been assayed using Illumina nucleotide sequencing from within the transposon into the adjacent target DNA. Using TraDIS (Transposon Directed Insertion-site Sequencing), we have been able to map ~550,000 unique transposon insertion sites to the Salmonella enterica serovar Typhimurium chromosome. This has allowed us to assay simultaneously every gene in the genome for essentiality and generate a genome-wide list of candidate essential genes.