GSE160069
Direct RNA-RNA interaction between Neat1 and RNA targets, as a mechanism for RNAs paraspeckle retention
The goal of this study was to determine if the lncRNA Neat1, in addition to its structural role, may also be directly involved in the binding by base pairing of RNAs and consequently in their retention in paraspeckles Overall design: GH4C1 sommatolactotroph pituitary cells (ATCC_ CCL-82.2™,  Molsheim, France) were grown in HamF10 medium supplemented  with 15% horse serum and 2% fetal calf serum. Cells were crosslinked with psoralen which only fix the RNA-RNA interactions. We then perfomed an RNA pull-down and RNAseq to determine the RNAs direcltely linked to Neat1