DRP006520
SA suppresses JA-responsive gene expression through the transcription cofactor NPR1.
Arabidopsis wild-type Columbia (Col-0), the jai3-1 and npr1-3 mutants were grown on vertical half-strength Murashige and Skoog plates in a growth chamber at 22?C under a 16 hr of light and 8 hr of dark cycle. Thirteen-day-old seedlings were dipped in water, 50 uM methyl-jasmonate, 0.5 mM sodium salicylate or a combination of SA and MeJA for 24 hr. Total RNA was isolated using the RNeasy Mini Kit.