GSE129766
Transcriptome sequencing analysis of LncMyod-knockdown satellite cells by RNA-seq
To understand the effect of LncMyod knockdown on cultured satellite cells, we isolated satellite cells from mouse hindlimb and cultured them in vitro with siRNA treatment targeting LncMyod. Following RNA-seq showed that loss of LncMyod leads to difficiencies in myogenic differentiation. Overall design: The polyA+ RNA from cultured satellite cells with siRNA treatment targeting LncMyod were sequenced using Illumina NextSeq 500 (2 x 150)