GSE175825
RNA-mediated activity-based protein profiling reveals the substrates of a human dihydrouridine synthase
We adapted the iCLIP method to map DUS3L-dependent DHU sites in a transcriptome-wide manner by treating the cells with 5-FUrd. Overall design: Flp-In T-Rex 293 cells expressing 3xFlag-DUS3L were treated with 5-FUrd, DUS3L-RNA were extracted and subject for Iclip library preparation.