3-enzyme-methylation
Genome-wide profiling of DNA methylation in human cancer cells
We have developed a methylation-specific digital sequencing (MSDS) method that can assess DNA methylation on a genomic scale. MSDS is a simple, low-cost method that combines the use of methylation-sensitive restriction enzymes with second generation sequencing technology. The MSDS method has the potential to provide novel insight into the functional complexity of the human genome.