Single_cell_transcriptome_of_schistosome_sporocysts-sc-6880
Single_cell_transcriptome_of_schistosome_sporocysts
Schistosoma mansoni eggs were isolated from the livers of infected mice, hatched in water and the free-living larvae miracidia collected and transformed into mother sporocysts (first intra-snail developmental stage) following the protocol described by Mann et al, 2010 (PMID: 19765348). The sporocysts were cultured for 5 days, collected, and proccesed for single cell isolation and 10X Chromium library generation and sequencing (Diaz et al, 2020. PMID: 33339816).   The samples for this study are:  Sporocysts samples   FUGI_R_D7465034 FUGI_R_D7465037 FUGI_R_D7465036 FUGI_R_D7465035  This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Schistosomiasis, a major Neglected Tropical Disease caused by the infection with blood flukes of the genus Schistosoma, affects more than 200 million people worldwide. To complete the life cycle, the parasite undergoes a sexual and asexual reproduction within a definitive mammalian host and a snail host, respectively. The intra-molluscan phase provides a critical parasite amplification step to ensure a successful transmission. However, the cellular and molecular mechanisms underlying the development of the intra-molluscan developmental stages are poorly understood. Single-cell transcriptomics provides an opportunity to define cellular types and investigate transcriptional signatures of specific cell populations in the first intra-snail stage of the parasite. Here, we collected and cultured schistosome mother sporocysts for 5 days and performed single cell sequencing to define the firs cell atlas of the intrasnail developmental stage of Schistosoma mansoni.