GSE175685
Transcriptomic profiling of THP-1 stable cell lines overexpressing genes associated with inflammatory bowel diseases (IBD)
More than 200 genomic loci have been identified and validated by GWAS to be associated with IBD. However, for most of these loci, the causal gene remains unknown. The current study is aimed at uncovering the role of genes located within IBD-associated loci and endogenously expressed in the myeloid cell model THP-1 by over-expressing them in this cell lineage. The open reading frames (ORF) for 42 genes from IBD-associated loci were expressed in the THP-1 model of human monocytes using lentivirus vector for plasmid transduction. An empty vector transduced into THP-1 as control. Three biological replicates have been done for each transduction. The impact of each IBD ORFs on THP-1 transcriptome profile was analyzed using an whole RNA sequencing-based approach. This functional genomics screen showed the affected genes (upregulated and downregulated) in response to the increased expression RNA of each IBD gene (ORF) in THP-1. In order to find biological categories enriched of the affected genes, enrichment analyses  have been done using the g:GOSt functional profiling tool (using Ensembl database release 98) from the online g:Profiler service. Overall design: mRNA profile of THP-1 overexpressing IBD genes