PRJNA860952
Elucidation of the genetic factors in Salmonella enterica Typhimurium involved in stop codon readthrough
Tn-seq was performed on the Salmonella enterica Typhimurium strain that carries a chloramphenicol-resistant gene with a premature stop codon as a reporter. The Tn5 mutants were selected on agar plates containing chloramphenicol to recover and characterize the mutants with increased stop codon readthrough.