PRJNA512427
Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
Dual Barcoded Shotgun Expression Library Sequencing (Dub-seq) is a method that uses random DNA barcodes to greatly increase experimental throughput. As a demonstration of this approach, we constructed a Dub-seq library with Escherichia coli genomic DNA, performed 155 genome-wide pooled fitness assays in 52 experimental conditions.