GSE139012
10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure
We thus isolated cortex from mice, with or without sevoflurane exposure, and performed single cell sequencing. Single cell sequencing is a innovative experimental procedures which allows the identification of various subsets of cells in the neverous system after sevoflurane exposure. Isolated cells were concentrated to 1000 cells/uL and approximately 17,000 cells were loaded into each channel to generate single-cell Gel Bead-In-Emulsions (GEMs), which led to expected mRNA barcoding of 10,000 single-cells for each sample. Seurat package was used for cell normalization and regression. Sevoflurane and control groups were aligned and combined using Seurat. Using t-distributed stochastic neighbor embedding (tSNE), 13 cluters of cells were visualized. Cells were assigned to various clusters based on strong expression of specific genes. Overall design: Examination of 2 different exposure in primary neurons