GSE113971
T47D Breast cancer cell line  ChIP-seq data II
We performed genome-wide  CDK9 ChIP seq experiments in T47D  cells  only expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2. Overall design: We designed experiment to perform genome wide CDK9 ChIP sequencing in T47D cells expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2.We find that PADI2 deiminates R1810 at CTD (C-terminal domian ) of RNAP2  and this is important to regulate the P-TEFb complex association with RNAP2. Therefore in order to investigate precise effect of the RNAP2  R1810A mutantion on CDK9  recruitment, we performed the CDK9 ChIP seq . Objective was to analyse  chromatin  binding regions of  CDK9 in T47D cells  expressing  wild type (WT) form of RNAP2 was expressed . After 24h of transfections , cells were treated  by 6ug/ml amanitin  to inhibit endogenous form of RNAP2 .So that we could demonstrate specific  CDK9 ChIP seq data  after expressing amanitin resistant wild type form  of RNAP2 .