Marine_Phage_Sequencing_Metagenomics_Strait_of_Georgia_Marine
3 samples from Strait of Georgia uncultured phage metagenome
GOB1A - isolated from nearshore estuary, photic zone, mixed layer; sample description: sampled off Jericho Pier, Vancouver, British Columbia.  PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration;  ultracentrifugation pelleting and phenol/chloroform DNA extraction  GOB2A - isolated from nearshore estuary, photic zone, mixed layer; sample description: sampled off Point Atkinson.  PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration;  ultracentrifugation pelleting and phenol/chloroform DNA extraction  GOB3A - isolated from coastal sea, photic zone, mixed layer; sample description: sampled during whale watching tour of coast of Vancouver Island.  PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration;  ultracentrifugation pelleting and phenol/chloroform DNA extraction