GSE173334
A Whole Transcriptome Profiling of Uterine Leiomyoma Cells under the influence of Phenolic Environmental Estrogen
Purpose:The study aimed to recognize potential molecular targets and signal pathways whereby phenolic environmental estrogen promotes the proliferation of uterine leiomyoma cells Methods:Primary cultured cell lines were  treated with 0.1% DMSO, 10.0 µmol/L BPA, and 32.0 µmol/L NP for 48 h before RNA-seq was performed. Differentially expressed genes affected by BPA and NP were selected. Then, Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and Protein-protein Interaction (PPI) analysis were performed. Quantitative real-time polymerase chain reaction (q-PCR) was used to verify the differentially expressed genes Results:In comparison with the control group, 739 differentially expressed mRNAs were identified in the BPA intervention group and the NP intervention group. GO enrichment analysis results show that the most enriched GO terms were connective tissue development and G1/S transition of mitotic cell cycle, and extracellular matrix. The results of KEGG enrichment analysis showed that differentially expressed mRNA was enriched mainly in three primary pathways, including environmental information processing, human diseases, and cellular processes. The cell cycle, PI3K-Akt signaling pathway, pathways in cancer, and TGF-beta signaling pathway are significantly enriched. Conclusions:Phenolic environmental estrogens may promote the proliferation and cell cycle progression of uterine leiomyoma cells through rapid non-genomic ER signaling, which leads to disordered cell cycle regulation and accelerates the transition of the cell cycle from G0/G1 phase to S phase. In addition, as an external harmful stimulant, phenolic estrogen promotes the upregulation of inflammatory factors in uterine leiomyomas. Overall design: Examination the mRNA profile of uterine leiomyoma cell lines treated with 0.1% DMSO , 10.0 µmol/L BPA, and 32.0 µmol/L NP for 48 h under the influence of Phenolic Environmental Estrogen.the cells treated with the 32.0 µmol/L NP are the control group.