PRJNA669023
Transcriptome profiling reveals cell specific changes enabling osteogenic differentiation in response to mechanical stimulation
RNA-Seq was performed on 12 samples of cells grown in culture. There were two cell types, and three culture conditions. Two biological repeats were performed with each cell type in each condition. Human Dermal Papilla cells (DP) or human Papillary Fibroblasts (PFi) were grown in growth media (GM), Osteogenic media (OM), or Osteogenic media plus shockwave stimulation (OMSW). 24 hours after media change cells were homogenized through QIAshredders and total RNA was isolated using RNeasy Plus micro kit prior to library construction using SMART-seq2 prepartion. 75bp paired-end sequencing was carried out on Illumina HiSeq 4000 instrument.