GSE113398
Potential role of hoxb1b in zebrafish development
identification of differentially expressed genes in hoxb1b homozygous mutant hindbrain when compared to wildtype in zebrafish Overall design: Total RNA was extracted from pools of dechrionated, deyolked of hoxb1b homzygous mutants and wildtype embryos at 18hpf using the RNeasy Mini Kit (Qiagen). Three libraries from wildtype embryos and three libraries from hoxb1b mutants were then generated from 3mg RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina). All libraries were analyzed for quality on a bioanalyzer prior to sequencing (Agilent 2100 BioAnalyzer).