E-MTAB-8001
Overexpression of RORA in primary mouse in vitro Th2
Naive CD4 T cells were taken from spleens of wild-type mice, and activated with CD3/CD28. IL4 was added to induce Th2. The cells were transduced with retrovirus to overexpress RORA vs control. Three different viral constructs were tested. M6-Rora2_mCherry appear to give the best result based on RNAseq validation of Rora expression. Rora2 = ENSMUST00000113624
Naive CD4 T cells were taken from spleens of wild-type mice, and activated with CD3/CD28. IL4 was added to induce Th2. The cells were transduced with retrovirus to overexpress RORA vs control. Three different viral constructs were tested. M6-Rora2_mCherry appear to give the best result based on RNAseq validation of Rora expression. Rora2 = ENSMUST00000113624