GSE176034
RNA-Seq analysis of Sfmbt depletion and ChIP-Seq analysis of Pho and Sfmbt-GFP binding in Drosophila ovaries
We analyzed the transcriptome of young ovaries by RNA sequencing (RNAseq) after germ line depletion of Sfmbt by RNAi. We determined the genomic binding sites for GFP-Sfmbt and Pho using ChIPseq. Overall design: Drosophila ovaries were dissected from 2 days old females after pupae eclosion. For RNAseq, it was used the nanos-Gal4 driver for germ line-specific expression of, respectively, UAS-mCherry RNAi (negative control) or UAS-Sfmbt RNAi (Sfmbt RNAi-1; TRiP.HMS00473). Three biological replicas were used for each condition. For Pleiohomeotic (Pho) ChIPseq, it was used anti-Pho antibodies on chromatin prepared from yw ovaries. Two biological replicas. For Sfmbt ChIPseq, it was used anti-GFP antibodies on chromatin prepared from GFP-Sfmbt or untagged control (Oregon-R) ovaries. Two biological replicas for each condition.