E-MTAB-9813
Transcriptomic analysis by AmpliSeq Ion Torrent to study the involvement of CXXC5 in the regulation of TGFß/BMP target genes in glioblastoma
The aim of this work was to elucidate the role of the transcription factor CXXC5 in glioblastoma and study its involvement in the regulation of TGFß and BMP target genes in this context. To this end we used the patient derived glioblastoma stem cells U3034MG that were either transfected with siRNA targeting CXXC5 or with a non-targeting pool (as control) followed by stimulation with either 5ng/ml TGFß or 30ng/ml BMP7 for 24 h. Then we analysed how silencing CXXC5 affects the expression of TGFß and/or BMP7 target genes using Ion torrent AmpliseqTM.
The aim of this work was to elucidate the role of the transcription factor CXXC5 in glioblastoma and study its involvement in the regulation of TGFß and BMP target genes in this context. To this end we used the patient derived glioblastoma stem cells U3034MG that were either transfected with siRNA targeting CXXC5 or with a non-targeting pool (as control) followed by stimulation with either 5ng/ml TGFß or 30ng/ml BMP7 for 24 h. Then we analysed how silencing CXXC5 affects the expression of TGFß and/or BMP7 target genes using Ion torrent AmpliseqTM.