GSE172354
Ribosome Profiling in Isoleucine tRNA Isoswitch Cells
Ribosome Profiling was used to evaluated whether changes in abundance of tRNAIleUAU and tRNAIleGAU would affect codon dependent translation and quantify dwell times over isoleucyl codons Overall design: Examination of ribosomal occupancy of isoleucyl codon enriched transcripts under Isoleucine tRNA modulation (increased tRNAIleUAU expression and decreased tRNAIleGAU expression via CRISPRi)