PRJNA549740
Assessing bacterial and fungal soil communities in composted biosolids amended turfgrass
This project examines the bacterial and fungal communities of composted biosolids amended soil at Eagle's Pride Golf Course (47.0841° N, 122.6692° W). Composted biosolids were generated by Earthworks (owned by Joint Base Lewis-McChord in Washington state) using biosolids generated on the military base, along with yard-debris and pre-consumer food waste. Soil samples were collected June 2015, prior to treatment as a baseline measurement. Post-application samples were collected in April and September 2016. The bacterial community structure was assessed by sequencing the V3-V4 region of the 16S rRNA gene (amplicons were generated with the forward primer, CCTACGGGNGGCWGCAG, and the reverse primer, GACTACHVGGGTATCTAATCC). The fungal community structure was assessed by sequencing the ITS1f-ITS2 region of the ITS rRNA gene (the amplicons were generate using the forward primer, CTTGGTCATTTAGAGGAAGTAA, and the reverse primer, GCTGCGTTCTTCATCGATGC).