GSE184795
RNAseq data for EndoC-ßH1 treated with CM from omental stromal cells cultured either in the absence or presence of TNF-a or differentiated into adipocytes
We used an in vitro model to study the paracrine effect of stromal cells isolated from omental adipose tissue at different stages of differentiation and inflammation on the beta cell lines EndoC-ßH1 through the use of conditioned media. We show that the expression of beta cells markers decreased and dedifferentiation markers increased when beta cells were cultured in conditioned medium derived from omental stromal cells. We report that stromal cells with a high pro-inflammatory profile had the most severe impact. By using RNAseq, we showed the stimulation of several signaling pathways such as STAT3, SMAD2 and RELA, as well as the downregulation of genes involved in lipogenesis and cholesterol synthesis. Overall design: RNAseq on EndoC-ßH1 cultured for 48h in CM prepared from stromal cells from 3 patients. CM was from omental stromal cells cultured in the absence (D0) or presence of TNF-a (D0TNFa) or differentiated into adipocytes (Ad). For the control medium (Ctr), EndoC-ßH1 were used for the 48h incubation