E-MTAB-8676
ATAC-seq of RS4:11 cell line populations that are unedited (SPI1 +/+), mono (SPI1 +/-) and biallelicaly edited (SPI1 -/-)
The goal of the experiment was to understand the epigenetic effects of PU.1 haploinsufficiency on pro-B cells.  The RS4:11 cell line was edited both mono and biallelicaly via electroporation of Cas9 and guides. Following editing, aliquots of unedited (SPI1 +/+), mono (SPI1 +/-) and biallellicaly edited (SPI1 -/-) cells were lysed before undergoing the transposition reaction. After transposition, the ATAC-seq libraries were purified and  then amplified via PCR. Libraries were sequenced using the Illumina Novaseq platform.
The goal of the experiment was to understand the epigenetic effects of PU.1 haploinsufficiency on pro-B cells.  The RS4:11 cell line was edited both mono and biallelicaly via electroporation of Cas9 and guides. Following editing, aliquots of unedited (SPI1 +/+), mono (SPI1 +/-) and biallellicaly edited (SPI1 -/-) cells were lysed before undergoing the transposition reaction. After transposition, the ATAC-seq libraries were purified and  then amplified via PCR. Libraries were sequenced using the Illumina Novaseq platform.