GSE108044
ketu mutant mice uncover an essential meiotic function for the ancient RNA helicase YTHDC2
Mechanisms regulating mammalian meiotic progression are poorly understood. Here we identify mouse YTHDC2 as a critical component. A screen yielded a sterile mutant, “ketu”, caused by a Ythdc2 missense mutation. Mutant germ cells enter meiosis but proceed prematurely to aberrant metaphase and apoptosis, and display defects in transitioning from spermatogonial to meiotic gene expression programs. ketu phenocopies mutants lacking MEIOC, a YTHDC2 partner. Consistent with roles in post-transcriptional regulation, YTHDC2 is cytoplasmic, has 3' to 5' RNA helicase activity in vitro, and has similarity within its YTH domain to an N6-methyladenosine recognition pocket. Orthologs are present throughout metazoans, but are diverged in nematodes and, more dramatically, Drosophilidae, where Bgcn is descended from a Ythdc2 gene duplication. We also uncover similarity between MEIOC and Bam, a Bgcn partner unique to schizophoran flies. We propose that regulation of gene expression by YTHDC2-MEIOC is an evolutionarily ancient strategy for controlling the germline transition into meiosis. Overall design: RNA-seq was performed on whole-testis RNA samples after polyA capture from three pairs of Ythdc2em1 mutant and wild-type littermates aged 8 dpp, three pairs of mutant and wild-type or heterozygous littermates aged 9 dpp and two pairs of mutant and heterozygous littermates aged 10 dpp. The six 8 and 9 dpp-old mutant and wild-type or heterozygous pairs were from six independent litters. The two 10 dpp-old mutant and heterozygous pairs were from a seventh litter. Animals were derived from crosses between heterozygote carriers, after five or six backcrosses of the Ythdc2em1 founder to B6 mice.