PRJNA560635
Genetic characteristics of Bursaphelenchus xylophilus
Total RNA was extracted separately from the powders of different stages of pine wood nematodes using TRIzol. Each RNA sample was sheared and reverse transcribed using random primers to obtain cDNA for library construction. The construction of the libraries and sequencing were all performed on a BGISEQ-500 RNA-seq platform (BGI, Shenzhen, China), and 50-bp single-end (SE) reads were generated.