GSE134223
Genomewide nucleotide-resolution mapping of single-strand breaks and lesions by GLOE-Seq [CDC9 inactivation]
Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3'-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3'-OH terminus. We demonstrate its applicability to the mapping of Okazaki fragments without prior size selection and detect biases and asymmetries in the distribution of spontaneous SSBs in budding yeast and human DNA. Overall design: IV. Mapping of Okazaki fragments in yeast   GLOE-Seq was applied to genomic DNA isolated from budding yeast under conditions of Cdc9 inactivation. A sample from a wild-type (ligase-competent) strain served as control.