GSE162230
RNA sequencing of ETV6-NCOA2 expression in CD34 positive cord blood cells
Mixed-phenotype acute leukemia is a rare subtype of leukemia in which both myeloid and lymphoid markers are co-expressed on the same malignant cells. Their pathogenesis is largely unknown, and their treatment is challenging. We have previously discovered the specific association between recurrent t(8;12)(q13;p13) chromosomal translocation, creating ETV6-NCOA2 fusion, with T/Myeloid leukemias. Here we report that the ETV6-NCOA2 initiates T/Myeloid leukemia in preclinical models and examine the expression profile induced by ETV6-NCOA2 in CD34+ cord blood progenitors in-vitro. Overall design: Gene expression examination of ETV6-NCOA2 or empty vector in CD34 positive cord blood cells in-vitro. 3 replicates each (for each biological replicate, at least three different cord blood samples were mixed)