ena-STUDY-Cambridge Sequencing Services (CSS)-09-04-2019-07:51:52:885-20
Sequences of low copy number transcripts from sugarcane cultivars N22 and SP80-3280 identified by PCR and sequenced with MinION. These transcripts support the selecton of low copy number genes for qRT-PCR experiments.
Based on genomic exome assemblies in the sugarcane cultivars LCP85-386 and SP80-3280 low copy number genes were chosen as potential references for qRT-PCR. Primers were designed from these assemblies and were validated experimentally.For each validated target the transcript sequences were amplified and sequenced in the sugarcane cultivars N22 and SP80-3280.
Based on genomic exome assemblies in the sugarcane cultivars LCP85-386 and SP80-3280 low copy number genes were chosen as potential references for qRT-PCR. Primers were designed from these assemblies and were validated experimentally.For each validated target the transcript sequences were amplified and sequenced in the sugarcane cultivars N22 and SP80-3280.