PRJNA510217
16S rDNA amplicon sequencing of Dermacentor nuttalli ticks
Amplification of V4-V5 hypervariable regions was performed as conventional PCR procedure. The primers 515F (5'- GTG CCA GCM GCC GCG GTA A -3') and 907R (5'- CCG TCA ATT CCT TTG AGT TT -3') used in amplifications also contained an 8 bp multiplex barcode and Illumina adapters.