PRJNA541176
CRISPR interference of Tnfsf11 gene transcription
CRISPR interference targeted to the Tnfsf11 transcription start site in mice was used as a global loss of function study to determine CRISPRi's efficiency in vivo. We performed RNAseq analysis on a mouse cell line with and without the CRISPRi knockdown of Tnfsf11 to investigate any off-target transcription effects.?