PRJNA801929
Single digest RAD-sequencing of Porites lobata from Palau
Between 2011-18, we collected tissue from Porites cf. lobata colonies using a hammer and chisel while on SCUBA. DNA was extracted using the Qiagen Blood and Tissue Kit. Genomic DNA concentrations were standardized 20 ng/ul. A total of 50 ul per sample was sent to Floragenex (Portland, Oregon) for single enzyme RAD library preparation with PstI enzyme digestion and were sequenced as 100 base pair single end reads across 6 lanes of an Illumina Hiseq 4000 using v4 chemistry at the University of Oregon Genomics Core facility.