ena-STUDY-ESALQ/USP Genomic Center-21-05-2021-13:42:31:935-325
The SAP30 genes was knocked out in mouse immortalizated myoblasts (C2C12 cell line).
The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was knocked down in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Knockdown of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. The DE analysis identified several upregulated genes annotated in muscle development in treated group (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation
The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was knocked down in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Knockdown of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. The DE analysis identified several upregulated genes annotated in muscle development in treated group (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation