PRJNA575574
Early fate of exogenous promoters in E. coli
The study evaluated the impact of random insertions of a reporter gene on gene expression and early fate of exogenous promoters in E.coli. Genomic DNA of single clones isolated from FACS sorted(based on GFP fluorescence) populations (QIAGEN DNA purification kit)bwere subjected for paired-end sequencing (2X75) using Illumina Miseq platform.