GSE98832
Reduction of rRNA during sRNA library construction using a set of blocking oligos, in Drosophila melanogaster
This experiment was designed to improve the proportion of miRNA mapping reads from sRNA library sequencing, by reducing the amount of particularly abundant rRNA fragments. Overall design: We extracted the sRNA fraction from 50 pooled male D. melanogaster accesory glands and constructed 4 cDNA libraries from the resultant RNA (blocked vs not-blocked with 2 biogical replicates). For the blocked libraries  we introduced several blocking oligos, with sequences complentary to the most abundant contaminating rRNA fragments, with the aim of preventing ligation of that rRNA to library adapters. The other libraries (unblocked) were constructed according to standard HD protocol. The libraries were sequenced on a HighSeq2500 platform.