GSE123970
Role for citron kinase in prostate cancer growth
To study the effect of siRNA-mediated CIT silencing on androgen-responsive genes in LNCaP cells. Overall design: Human LNCaP prostate cancer cells were transfected with siRNA SmartPools targeting CIT or a non-targeting siRNA SmartPool.  Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (0.1nM) or vehicle.  Three biological replicates were generated per treatment group.  Forty-eight hours later, total RNA was isolated and processed for RNA-Seq analysis.