GAO_CF
The prevalence of streptococci and increased polymicrobial diversity associated with cystic fibrosis patient stability
Diverse microbial communities chronically colonize the lungs of cystic fibrosis patients.  Pyrosequencing of amplicons for hypervariable regions in 16S rRNA generated taxonomic profiles of bacterial communities for sputum genomic DNA samples from 22 patients during a state of clinical stability (outpatients) and 13 patients during acute exacerbation (inpatients). We employed qPCR to confirm the detection of Pseudomonas aeruginosa and Streptococcus by the pyrosequencing data, and HOMIM analysis to speciate the streptococci identified by pyrosequencing. We show that outpatient sputum samples have significantly higher bacterial diversity than inpatients, but treatment with tobramycin did not impact overall diversity. The increased fractional representation of Streptococcus in the outpatient cohort relative to the inpatient cohort was the strongest predictor of clinically stable lung disease. The most prevalent streptococci included species typically associated with the oral cavity (S. salivarius and S. parasanguis) and the Streptococcus milleri group species. These species of Streptococcus may play an important role in increasing the diversity of the cystic fibrosis lung environment and promoting patient stability.