PRJNA418693
Combined transcriptomic and proteomic analysis of chicken DF-1 cells following innate immune stimulations
The goal of our study was to experimentally quantify both at the transcriptomic and proteomic level the effects of comprehensive innate immune stimulations in chicken. To this end, SILAC-labeled chicken DF-1 cells were mock-treated or treated with either ChIFN1 (1000 IU/mL for 6h), transfected poly (I:C) (4,25 ug/mL for 6 h), Infectious Bursal Disease Virus strain PBG-98 (IBDV, MOI 5 for 8h), Fowlpox Virus strain FP9 WT, fpv012 or fpv014 mutants (FWPV, MOI 5 for 8h). Total cellular RNA was extracted using RNeasy Mini Kit (QIAGEN), Illumina libraries were prepared and sequenced. This Bioproject contains the raw sequencing data from this study.