PRJNA679400
PTEN Upstream Open Reading Frame Encodes A Circuit Breaker of Lactate Metabolism
Total genomic DNA was extracted from samples, genomic DNA was fragmented using NEBNext dsDNA Fragmentase (NEB, Ipswich, MA, USA) following by DNA ends repairing. Endrepaired DNA fragments were dAtailed and ligated with the NEBNext adapto (NEB, Ipswich, MA, USA). Biotinylated RNA library baits and magnetic beads were mixed with the barcoded library for targeted regions selection using the SureSelect Human All Exon V6 Kit (Agilent Technologies, Palo Alto, Calif.). The captured sequences were further amplified for 150bp pairedend sequencing in Illumina Nova-seq system (Illumina, San Diego, CA, USA).