ena-STUDY-PASTEUR-19-11-2014-10:18:27:751-1486
Whole Genome Sequence and Molecular Characterization of phiCD505, a Myoviridae Phage Infecting Clostridium difficile
Clostridium difficile strains have been investigated for the presence of functional prophages. Following mitomycine C induction, temperate phages have been isolated. Transmission electron microscopy (TEM) has revealed member of Siphoviridae / Myoviridae family. Phage DNA has been purified following phage amplification on sensitive host and subjected to complete genome sequencing using Illumina technology. Following sequencing, double stranded-DNA (dsDNA) genomes have been assembled and annotated.
Clostridium difficile strains have been investigated for the presence of functional prophages. Following mitomycine C induction, temperate phages have been isolated. Transmission electron microscopy (TEM) has revealed member of Siphoviridae / Myoviridae family. Phage DNA has been purified following phage amplification on sensitive host and subjected to complete genome sequencing using Illumina technology. Following sequencing, double stranded-DNA (dsDNA) genomes have been assembled and annotated.