CA in Kelpies
Investigation of DNA polymorphisms in the cerebellar abiotrophy region in Australian Kelpie dogs
Cerebellar Abiotrophy (CA) is an autosomal recessive disease in Australian kelpie dogs that results in ataxia. The disease locus was previously mapped to a 5 Mb region on canine chromosome 3 (Shearman et al 2011, Animal Genetics). Nimblegen sequence capture and 454-sequencing of this 5 Mb candidate region was performed for two affected and one unaffected kelpie using multiplex identifier sequences on one picotitre plate. MID1: ACGAGTGCGT - Affected 6025* MID2: ACGCTCGACA - Affected 6065* MID3: AGACGCACTC - Unaffected 6074* * Sample names are from the publication Shearman et al 2011, Animal Genetics.
Cerebellar Abiotrophy (CA) is an autosomal recessive disease in Australian kelpie dogs that results in ataxia. The disease locus was previously mapped to a 5 Mb region on canine chromosome 3. Nimblegen sequence capture and 454-sequencing of this 5 Mb candidate region was performed for two affected and one unaffected kelpie using multiplex identifier sequences on one picotitre plate.