ena-STUDY-ROSKILDE UNIVERSITY-07-01-2019-14:39:20:306-605
The genome assembly of the harpacticoid copepod Tisbe holothuriae. PCRfree WGS reads was assembled using SPAdes and scaffolded with the HAHV01 mRNA transcriptome.
Here, we present the first genome resource from the surface associated harpacticoid copepod Tisbe holothuriae. Animals in all life stages were sampled from a culture and any non-copepod particles removed by repeated rinsing as well as by manual removal of particular matter. DNA was purified using DNeasy from Qiagen and the Truseq PCRfree kit was used to produce Illumina sequencing libraries. 2x150nt Illumina Nextseq 500 technology was used for sequencing and the resulting sequences were assembled using SPAdes. Contigs were scaffolded using L_RNA_scaffolder and the HAHV01 mRNA transcriptome.
Here, we present the first genome resource from the surface associated harpacticoid copepod Tisbe holothuriae. Animals in all life stages were sampled from a culture and any non-copepod particles removed by repeated rinsing as well as by manual removal of particular matter. DNA was purified using DNeasy from Qiagen and the Truseq PCRfree kit was used to produce Illumina sequencing libraries. 2x150nt Illumina Nextseq 500 technology was used for sequencing and the resulting sequences were assembled using SPAdes. Contigs were scaffolded using L_RNA_scaffolder and the HAHV01 mRNA transcriptome.