GSE104910
Differentially Expressed Genes upon Knockdown of ZRANB1 or EZH2 in LM2 Cells
EZH2, the catalytic component of the Polycomb repressive complex 2 (PRC2), silences gene transcription by methylating histone H3 at lysine 27. Recently we identified ZRANB1 as the EZH2 deubiquitinase. ZRANB1 binds, deubiquitinates, and stabilizes EZH2. To determine whether ZRANB1 is a functional regulator of EZH2, we performed RNA-Seq analysis to compare the effect of ZRANB1 knockdown or EZH2 knockdown on global gene expression. Overall design: mRNA profiles of control, ZRANB1 knockown, or EZH2 knockdown LM2 cells were generated from triplicate samples using RNA-Seq