GSE111379
6mer seed toxicity in tumour suppressive microRNAs
6mer seed toxicity in tumour suppressive microRNAs Overall design: Samples of human ovaries were generated by deep sequencing, using Illumina HiSeq4000.  HeyA8 cells treated with Doxorubicin, Carboplatinum, Etoposide. We provide evidence that miR-34a is toxic to cancer cells mostly through its toxic 6mer seed sequence. RNA isolated from HeyA8 cells 48 hrs after transfection with either a nontargeting siRNA (siNT2), miR-34a Seed, pre-miR-NC, or pre-miR-34a was subjected to deep sequencing, using Illumina HiSeq4000.