ena-STUDY-EMBL-23-11-2017-23:19:18:588-14
High-throughput yeast CRISPR genome editing
Here we describe a CRISPR/Cas9-based platform in S. cerevisiae for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC), which employs array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and enable robust phenotyping.
Here we describe a CRISPR/Cas9-based platform in S. cerevisiae for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC), which employs array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and enable robust phenotyping.