GSE183282
Circadian rhythms of ribosome binding for Drosophila astrocyte RNAs
Raw RNAseq data (fastq) files that were analyzed for circadian changes in RNA ribosome binding. The numbers in the file names (00 - 44) represent circadian time (0 to 44 hours in constant dark and constant temperature). Two separate sets of samples (independent biological replicates) were collected over a 44-hour period of time. Overall design: Translating ribosome affinity purification (TRAP) methods were employed to purify ribosomes and their RNA cargoes from Drosophila astrocytes. The Gal/UAS procedure was used to express a tagged ribosome (EGFP-L10a) in fly astrocytes in order to conduct TRAP. Four independent sets of timepoints (00-44 hours in constant conditions at 4 hour intervals) were sequenced in the study. These timepoints represent 2 biological replicates (2 technical replicates for each). Sets 1 and 2 below represent one biological replicate while sets 3 and 4 represent the other.