GSE133114
Widespread binding of TFIID and SAGA to yeast promoters mapped by an improved ChEC-seq method
Mittal and colleagues have questioned the validity of mapping transcription factor locations on DNA using the MNase-based ChEC-seq method. Here, we present an updated ChEC-seq approach with modified experimental conditions and computational analysis. The revised method overcomes the objections of Mittal et al and identifies widespread promoter binding of the transcription coactivators TFIID and SAGA with no preference for gene category – consistent with our earlier conclusions. We also show that the revised method is suitable for mapping the genome-wide locations of sequence-specific factors. Overall design: A modified ChEC-seq method was used to map genome-wide binding of TFIID and SAGA. To validate this modified ChEC-seq approach we also mapped well studied activators Abf1 and Rap1. Experiments for TFIID and SAGA were done in four replicates (A,B,C,D) and experiments for Abf1 and Rap1 were done in duplicates (A,B).