GSE130170
The effects of different chemical forms of iron fertilizer on cadium accumulation in dwarf Polish wheat are reflected in the differentially expressed genes
Purpose: To understand the effects of two different chemical forms of iron fertilizer on cadmium accumulation Methods:Cultivation and treatment for three weeks of dwarf Polish wheat seedlings by hydroponics, in triplicate, qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: Iron fertilizer can effectively reduce cadmium concentration in plants Conclusions: Our study represents the different chemical forms of iron fertilizer have different mitigation effects on cadmium. The transcriptome gata showed that iron fertilizer have changed the cadimium metabolism Overall design: The groups treated with two fertilizers and CdCl2 solution before collected.The samples were collected after one week treatment. The roots samples were immediately frozen in liquid nitrogen and stored at -80°C.It's three biological replicates per treatment for subsequent RNA extraction and analysis.