Clione limacina  neuronal transcriptome
Clione limacina  neuronal transcriptome
We developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.
The pteropod mollusc, Clione limacina is a well-recognized model to study cellular bases of behavior and the organization of central pattern generators in particular. However, nothing is known about the molecular organization of its nervous system. Here, using pyrosequencing approach we performed analysis of transcripts expressed in the central nervous system of Clione. Specifically, we developed a reduced representation sequencing method that can detect low abundant transcripts. Our method greatly reduces the amount of sequencing with more accurate quantification. Basically, sequential addition of adaptors paired with controlled digestion of cDNA generates a one transcript on read type of analysis. Combined 5’ with 3’ targeted libraries produces greater coverage of individual transcripts and high quality assembly.   Our initial analysis allowed us to identify more than 10,000 of neuronal transcripts including a diversity of receptors and other components of signal transduction pathways essential for future analysis of molecular bases of neuronal functions in this model organism.