ena-STUDY-CHUV-UNIL-08-11-2018-15:33:10:415-1086
Thus study describes the differences in genomes of Candida lusitaniae isolates
Different isolates were obtained during therapy and they were assigned to 5 different antifungal susceptibility profiles (P1-P5). The genomes of isolates P1 to P5 were subjected to whole genome sequencing using the PacBio technology in order to resolve mutations associated with antifungal resistance. The genomes were annotated with a combination of systematic blasts and use of RNAseq data and compared with each other.P1 was the earliest isolate obtained from stools at initiation therapy and was susceptible to all antifungals. P2, P3, P4 and P5 were MDR isolates since they exhibited resistance to at least 2 drug classes. These isolates originated from the bloodstream indicating a systemic infection likely originating from P1 in the gastro-intestinal tract. Assembly of P1 to P5 genomes could resolve 8 large contigs that corresponded to the 8 chromosomes reported in C. lusitaniae. P1 to P5 genomes did not undergo significant rearrangements and were related to P1. The PacBio assembled genomes were next compared to each other and with the initial isolate P1. From a total of 13 non-synonymous SNPs (NSS) in P1 to P5 comparisons, 6 were associated with Multidrug resistance (MDR).
Different isolates were obtained during therapy and they were assigned to 5 different antifungal susceptibility profiles (P1-P5). The genomes of isolates P1 to P5 were subjected to whole genome sequencing using the PacBio technology in order to resolve mutations associated with antifungal resistance. The genomes were annotated with a combination of systematic blasts and use of RNAseq data and compared with each other.P1 was the earliest isolate obtained from stools at initiation therapy and was susceptible to all antifungals. P2, P3, P4 and P5 were MDR isolates since they exhibited resistance to at least 2 drug classes. These isolates originated from the bloodstream indicating a systemic infection likely originating from P1 in the gastro-intestinal tract. Assembly of P1 to P5 genomes could resolve 8 large contigs that corresponded to the 8 chromosomes reported in C. lusitaniae. P1 to P5 genomes did not undergo significant rearrangements and were related to P1. The PacBio assembled genomes were next compared to each other and with the initial isolate P1. From a total of 13 non-synonymous SNPs (NSS) in P1 to P5 comparisons, 6 were associated with Multidrug resistance (MDR).