DRP008559
Human and murine skin microbiota of atopic dermatitis
Human skin swab samples were collected from Japanese healthy volunteers and patients with atopic dermatitis (AD). Swab samples were also collected from neck skin of SPF Tmem79-/- AD model mice and Tmem79-/- mice colonized with complex AD microbiota. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27F and 354R) and, multiplexed amplicon pyrosequencing was carried out using MiSeq (illumina).
Human skin swab samples were collected from Japanese healthy volunteers and patients with atopic dermatitis (AD). Swab samples were also collected from neck skin of SPF Tmem79-/- AD model mice and Tmem79-/- mice colonized with complex AD microbiota. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27F and 354R) and, multiplexed amplicon pyrosequencing was carried out using MiSeq (illumina).