PRJNA527834
Transcripts transferred in extracellular vesicles between pre-implantation human embryo and the endometrium
Spheroids derived from JAr (human choriocarcinoma) cells were used as an in vitro analogue for human trophoblast. RNA of the trophoblast spheroids were tagged with 5-ethynyl uridine. Tagged spheroids were co-incubated with human endometrial cell analogue (RL 95-2 cell line monolayer). Endometrial RNA was extracted, transferred tagged RNA was affinity precipitated and sequenced.