PRJNA264431
Mus musculus strain:B6.129S-Atoh1-GFP Targeted Locus (Loci)
To evaluate the DNA cleavage efficiency of Cas9 delivered as protein complexed with either EMX sgRNA or GFP sgRNA in liposomes to the mouse cochlea in vivo, we measured the modification of on-target genomic target sites EMX or GFP. The target sites were amplified from genomic DNA isolated 10 days post treatment.