GSE162305
Conventional RNA-seq and 3'end-seq data from HEK293T
To inverstigate off-target effects of CRISPR-iPAS, we transfected HEK293TAPA reporter cells with dPguCas13b and gUSE (showed most effective APA-interference) or non-targeting gRNA (gNT) respectively. 3'end-seq and RNA-seq were performed to investigate if the global change on the endogenous PAS usage and gene expression caused by CRISPR-iPAS. Comparing with gNT, dPguCas13b with gUSE showed high specificity. Overall design: 3'end-seq and RNA-seq were performed on HEK293TAPA cells co-transfected with dPguCas13b and gUSE or gNT respecitively.