E-MTAB-7332
ChIP-Seq data from Nup133 ChIP performed on primary male and female mouse derived oligodendrocyte precursor cells
Nup133 is known to play a crucial role in neural cell differentiation. But its downstream targets and the exact mechanisms of its action are largely unknown. Hence, we performed targeted chromatin immunoprecipitation experiments followed by next generation sequencing to identify its genomic targets.  Mouse OPCs that were isolated separately from male and female littermates were subjected to ChIP using anti-rabbit NUP133 Polyclonal antibody. The prepared ChIP output DNA from 4 individual bio-replicates, along with an input control for each sample was used to prepare Illumina TruSeq nano ChIP sequencing libraries. The Illumina NextSeq 500 platform and a high-output v2 1x75bp cycles kit were used to sequence the Illumina TruSeq ChIP libraries.
Nup133 is known to play a crucial role in neural cell differentiation. But its downstream targets and the exact mechanisms of its action are largely unknown. Hence, we performed targeted chromatin immunoprecipitation experiments followed by next generation sequencing to identify its genomic targets.  Mouse OPCs that were isolated separately from male and female littermates were subjected to ChIP using anti-rabbit NUP133 Polyclonal antibody. The prepared ChIP output DNA from 4 individual bio-replicates, along with an input control for each sample was used to prepare Illumina TruSeq nano ChIP sequencing libraries. The Illumina NextSeq 500 platform and a high-output v2 1x75bp cycles kit were used to sequence the Illumina TruSeq ChIP libraries.