GSE200480
Small RNA sequencing of regenerating mouse hindlimb muscle and Type 1-Lang reovirus-infected mouse heart
Small RNAs, including microRNAs, play many roles in the regulation of gene expression. We performed small RNA-sequencing on two groups of samples to catalog which microRNAs are expressed during muscle regeneration in response to chemical injury and in heart tissue in response to viral-induced myocarditis. Overall design: For skeletal muscle samples, following the injury time course, tibialis anterior muscles were dissected and snap frozen with liquid nitrogen. The Norgen Total RNA Purification Kit (Cat. 17200) was used to extract RNA from 10 mg of tissue for each sample. For heart samples, following the infection time course, hearts were dissected, embedded in OCT, and frozen in liquid nitrogen. RNA was extracted with Trizol (Invitrogen, Cat. 15596026) and glycogen precipitation for a small fraction of each of the heart samples. RNA quality was assessed via High Sensitivity RNA ScreenTape Analysis (Agilent, Cat. 5067-5579) and all samples had RNA integrity numbers greater than or equal to 7. Small RNA sequencing was performed at the Genome Sequencing Facility of Greehey Children's Cancer Research Institute at the University of Texas Health Science Center at San Antonio. Libraries were prepared using the TriLink CleanTag Small RNA Ligation kit (TriLink Biotechnologies, San Diego, CA). Libraries were sequenced per lane with single-end 50× using a HiSeq2500 (Illumina, San Diego, CA).