GSE106814
LIN-41 RIP-Seq
FLAG-GFP tagged LIN-41 was immunoprecipitated using FLAG dynabeads (Sigma) from lysates of young adult C.elegans. After washing steps, bound fraction (IP) was eluted from beads and associated RNAs were isolated by using the Pico pure RNA isloation kit (Invitrogen) Overall design: RNA isolated from Input and IP fractions were sequenced for two biological replicates to identify RNAs specifically enriched in IP fraction