ena-STUDY-CME-02-07-2013-16:54:18:874-190
buk defined community sequence data from 454 GS FLX Titanium platform and 454 GS Junior platform
To test the pyrosequencing technology and analysis pipeline under specific conditions, we use a buk defined community with genomic DNAs fromClostridium perfringens ATCC 13124, C. difficile 630, C. acetobutylicum ATCC 824, Bacillus licheniformis 14580 and Bacteroidetes thetaiotaomicron E50. The DNA was amplified separately using three barcoded forward and three reverse primers and was sequenced on 454 GS FLX Titanium platform and 454 GS Junior platform.
To test the pyrosequencing technology and analysis pipeline under specific conditions, we use a buk defined community with genomic DNAs fromClostridium perfringens ATCC 13124, C. difficile 630, C. acetobutylicum ATCC 824, Bacillus licheniformis 14580 and Bacteroidetes thetaiotaomicron E50. The DNA was amplified separately using three barcoded forward and three reverse primers and was sequenced on 454 GS FLX Titanium platform and 454 GS Junior platform.