GSE128055
Gene expression profile during senescence induced by LSG1 knockdown
In the present study, we analyze the effect of knocking down LSG1 and KRas(V12D) overexpression in MRC5 cells  in the transcriptome using Ampliseq RNA sequencig. We observed that shLSG1 induced a potent senescence response that is characterized by the activation of ER-Stress and cholesterol biosynthetic pathway Overall design: MRC5  were transfected with siRNA to knockdown the small GTPase LSG1. Total mRNA was extracted and expression profiles were analyzed.