PRJNA675083
Genome sequencing of CRISPR-Cas9 nickase generated clpP1 and clpP2 mutants in Clostridioides difficile strain 630
We used a CRISPR-Cas9 nickase genetic editing system to insert stop codons into the clpP1 and clpP2 genes in Clostridioides difficile strain 630. The genomes of each mutant were re-sequenced to confirm the absence of any off targets.