PRJNA874829
soil  metagenome
All sampling instruments were sterilized and disinfected. The plants were gently shaken from the soil to remove loosely attached soil particles. Alternatively, the rhizosphere soil was scraped with a sterilizing blade and immediately placed in a centrifuge tube containing 20 ml phosphate buffer. After centrifugation, visible impurities and plant tissues were removed before transferring the sample to a 15 ml sterile tube and immediately fixing it with liquid nitrogen for DNA extraction. Each treatment had four replicates, and each replicate consisted of 10 apple seedlings. SITE: Hebei Agricultural University, Baoding City, Hebei Province.