PRJNA803941
Methylation at the rs12946510 locus in the CEM human T cell line subjected to CRISPR/Cas9 genome editing
Alternative genotypes of rs12946510 were introduced into the genome of the CEM human T cell line using the two-step CRISPR/Cas9 method of Kazuya Ikeda et al. (2018, PMID: 30504872) to confirm gene targets of this regulatory SNP. Methylation levels were evaluated in a CpG island and two hypomethylated regions which are close to or overlap rs12946510.