GSE125469
Effect of circPTPRM on the biological behaviors of hepatocellular carcinoma cells in vitro and its clinical significance.
In this paper, a new circRNA was discovered by RNA sequencing, and its relationship with hepatocarcinoma proliferation, apoptosis, invasion and migration and clinical correlation of hepatocellular carcinoma were verified from different angles, providing a basis for further discussion on the mechanism of promoting the occurrence and development of hepatocellular carcinoma. Overall design: RNA sequencing was performed on 3 pairs of hepatocellular carcinoma and their adjacent paired tissues, and 3 differentially expressed circRNAs were selected and verified by Real time PCR in 30 pairs of tissue samples, hepatoma cells and normal liver cells, and clinical data were analyzed. From which candidate circRNAs are selected. Then, the anti-candidate circRNA si RNA-1 and si RNA-2 were transfected into the hepatoma cell line, and the biological behavior of the hepatoma cells after silencing of the candidate circRNA was detected by cell proliferation, plate cloning, flow cytometry and transwell.