GSE129657
The chromatin landscape modified by Zbtb18 in activated B cells
The differentiation of plasma cell is prescisely controlled by the key transcriptional program as well as epigenetic changes. Recently, we have identified, one zinc finger protein, Zbtb18, as a new regulator for B cell differentiation process. To investigate the molecular mechanisms of Zbtb18 during B cell differentiation program we employed the assay for transposase (Tn5) -accessible chromatin followed by deep sequencing (ATAC-Seq) in the sorted Control and Zbtb18 over-expressing B cells (B220+ CD138-) before and after anti-CD40 and IL-4 stimulation. Mice primary B cells were retrovially transduced  with either control vector with RFP repoter or Zbtb18 with GFP repoter, and both were primed 2 days on the 40LB stroma cells before mixed 1:1 and then stimulated with anti-CD40 (10ug/ml) and IL-4 (10ng/ml) for 2 days. Activated B cells, B220+ CD138-, were sorted by BD AriaIII and followed by ATAC-seq protocol (Nat Methods. 2017 Oct;14(10):959-962). Overall design: ATAC-seq in control and Zbtb18 over-expressing B cells, stimulated with anti-CD40 and IL-4 for 2 days (n=3) or unstimulated (n=2)