In_vivo_RNA_interference__RNAi__target_sequencing_of__Trypanosoma_brucei-sc-2012-10-09T11:26:57Z-2391
In_vivo_RNA_interference__RNAi__target_sequencing_of__Trypanosoma_brucei
A direct comparison of RNAi in vitro with RNAi in vivo is being performed using RNA interference (RNAi) target sequencing (RIT-Seq) of  Trypanosoma brucei to identify all genes specifically required for growth in vivo (the ‘infectome’). Assembly of the bloodstream-form T. brucei RNAi library and the RNAi target sequencing (RIT-seq) approach in African trypanosomes were reported previously  in Alsford, S. et al. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res 21, 915-924, 264 doi:gr.115089.110 [pii] 265 10.1101/gr.115089.110 (2011) and Alsford,S et al. High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232–236 doi:10.1038/nature10771 (2012). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
In vivo RIT-seq screen of Trypanosoma brucei to identify all genes specifically required for growth in vivo (the ‘infectome’).