PRJNA664221
Insecticidal activity of Bacillus thuringiensis crystal proteins, Cry2Aa and Cry1Fa, on symbiotic bacterial microbes, in-vitro raised spiders of Pardosa astrigera (Lycosidae, Araneidae)
In-vitro studies via exposing laboratory grown 5th instar spiderlings of non-target spider species P. astrigera to Cry2Aa and Cry1Fa Bt proteins via Bt fed bollworms larvae till to their adult hood age. Effects of tested Bt proteins was inspected through survival, number of days engaged by 5th instars to reach their succeeding growth stages and weight gain over preceding growth period and then their extracted DNA subjected to 16S rRNA and high-through put sequencing for the influence of tested Bt proteins on biodiversity of bacterial symbionts of the spider species.