GSE136079
RNA-seq analysis of Actn2 knockout in mouse cardiomyocytes
The goal of this study is to analyze the impact of Actn2 depletion on transcription during cardiomyocyte maturation in mouse Overall design: Actn2F/F and Actn2F/+ mice were injected with AAV9-cTNT-Cre specifically in cardiomyocytes at P1.  Five biological replicates were analyzed in each group. In each experiment, cardiomyocytes were isolated by retrograde collagenase perfusion and purified by fluorescence activated cell sorting. Total RNA was extracted from FACS sorted cells. RNA-seq libraries were generated using SMART-seq and Nextera kits and were sequenced on a NextSeq550 sequencer. FASTQ Data were aligned by STAR and count matrix generated by FeatureCounts.