Study-16S_rRNA_mouse_colon
Spatial organization of the intestinal microbiota in the mouse ascending colon
The proximal murine colon contains mucosal folds that are known to be associated with morphologically distinct microbes. To identify these microbes, we utilized the technique of laser capture microdissection to procure microbes associated with these folds (interfold region) and within the central lumen (digesta region). Microbial populations were characterized by 16S rRNA gene tag pyrosequencing.