PRJNA498234
Characterization of the var gene associated GC-rich non-coding RNA in Plasmodium falciparum
In this study we characterized the GC-rich non-coding RNA (gcRNA) which is located upstream of central var genes in the human malaria parasite Plasmodium falciparum. We knocked-down the gcRNA using CRISPRi and assessed transcriptomic changes using stranded RNA seq. Positive binding of the dCas9 protein to the target locus was characterized by dCas9-ChIP seq