H. Influenzae Sequencing
Natural Transformation in H. Influenzae
A KW20 laboratory isolate of H. influenzae (Rd-RR) was transformed using standard methods with an antibiotic-resistant derivative of the clinical isolate 86-028NP (NP-NN).  Both donor and recipient genomic DNA was sequenced, along with four transformed recombinant isolates.  Two recombinants (Nov1 and Nal1) were sequenced individually, while all four recombinants (Nov1, Nal1, Nov2, and Nal2) were sequenced as an unbarcoded pool.
We performed Illumina paired-end sequencing of H. Influenzae to study the process of natural transformation.