GSE99578
Small RNA sequencing of murine pancreatic tumors driven by polyoma middle-T antigen with wild-type or single-copy loss of Dicer
We report the small RNA sequencing results of mouse pancreatic tumor cell lines driven by polyoma middle-T antigen, which have additionally been engineered to have either wild-type (herein "PDwt") or single-copy loss (herein "PDhet") of the Dicer gene. As expected, PDhet cell lines exhibit lower miRNA levels compared to PDwt cell lines. Principle component analysis, detailed in the published manuscript, reveals PDwt cell lines to be more widely varying in miRNA signature compared to PDhet cell lines. Grouping miRNAs by family reveals several families to be significantly downregulated in PDhet cells lines below 50% of PDwt levels, including miR-10, -148, -17, -28, -299, -30, -34, and -379. Overall design: 50-cycle single-end reads using the Illumina platform were performed on small RNAs isolated from low-passage tumor cell lines derived from Elastase-tva, Trp53(fl/fl), Dicer(wt/wt), p48-Cre or Elastase-tva, Trp53(fl/fl), Dicer(fl/wt), p48-Cre mice which had been infected with RCAS-Polyoma middle-T virus at postnatal day 3.