PRJNA671607
Tracking DNA synthesis during break-induced replication reveals key mechanistic insights and stalling at roadblocks
Interstitial telomere sequences (ITSs) as one of the CFSs has been demonstrated to causing genomic instability during normal DNA replication but how it affects DNA synthesis during Break-induced replication (BIR) is still unknown. Using our yeast system, we inserted an ITS sequence on the template of BIR, and observed a pre-matured BIR product, which didn't pass through the ITS position.