PRJNA577547
Spider microbiome
DNA extracted from different tissue types of the European wasp spider, Argiope bruennichi, was amplified with primer pairs targeting the V4 region of the 16S rRNA (515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806r: 5'-GGACTACNVGGGTWTCTAAT-3' (Walters et al., 2015) coupled to custom adaptor-barcode constructs. PCR amplification and Illumina MiSeq library preparation and sequencing (V3 chemistry) was carried out by LGC Genomics in Berlin.