GSE125121
RNA sequencing of host transcriptome of human foreskin fibroblast cells infected with either bradyzoites and tachyzoite of Toxoplasma gondii
The purpose of this data set is to determine if bradyzoites impact the host transcripton in a similar manner as tachyzoites. We found that 3853 differentially expressed genes (DEGs) are shared between tachyzoite infected cells and bradyzoite infected cells (41%; 2161 up-reg., 1692 down-reg.) when compared to uninfected host samples, leaving 3356 tachyzoite specific DEGs (1488 up-reg., 1868 down-reg.), and 1951 DEGs specific to bradyzoite infection (1075 up-reg., 876 down-reg).  We conclude therefore that bradyzoites manipulate the host cell and that this is unique when compared to tachyzoites. Overall design: Four groups of human foreskin fibroblasts are compared. These samples are either uninfected or infected with Toxoplasma gondii. These data comprize 12 samples, all taken from a single donor, with 3 samples per group. The samples are either uninfected host (24 hour incubation), uninfected host (1 week incubation), infected with the tachyzoite stage of Toxoplasma (24 hour incubation), or infected with the bradyzoite stage of Toxoplasma (1 week incubtion)