GSE122101
RNA sequencing data of Ssu72 Wild Type and Knock-Out MEFs, hepatocytes and ES cells
To investigate how mammalian Ssu72 regulated gene expression, we performed RNA-Seq analyses of Ssu72 in three different cell types depending on their proliferation capabilities: embryonic stem (ES) cells (highly proliferating), primary murine embryonic fibroblasts (MEFs) (moderate proliferating), and hepatocytes (almost quiescent). Ssu72 deletion was induced by infection with Cre recombinase adenovius (Ad-Cre). Infection of ESf/f, MEFf/f, and hepatocytef/f with Ad-Cre resulted in efficient reduction of Ssu72 levels. Depletion of Ssu72 resulted in downregulation or upregulation of large subsets of genes in three different cell types. Overall design: MEFs, hepatocytes and ES cells mRNA profiles of 6-weeks old wild type (WT) and Ssu72 knock-out (KO) mice were generated by deep sequencing, in duplicate or triplicate, using Illumina GAIIx.