ena-STUDY-ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESEARCH-27-01-2017-16:22:33:197-239
Complete chloroplast genomes and mitochondrial sequences of larches from shotgun sequencing
High latitudinal ecosystems are strongly climate-related and vegetation models project a densification and northward expansion of the Siberian larch taiga into areas currently covered by Arctic tundra. The genetic diversity and dynamics of larch populations at the tundra-taiga ecotone, the transition zone from light taiga to tundra, are largely unknown due to the remoteness and difficult accessibility. The goal of our study was to use shotgun sequencing of needle-derived total DNA to assemble the complete paternally-inherited chloroplast genomes as well as maternally-inherited mitochondrial sequences of 12 individuals from the range of Larix gmelinii and 7 from the range of Larix cajanderi and identify genetic variation. The chloroplast genomes result in a circular sequence of 122,581-122,593 bp, depending on InDels and length differences of homopolymer stretches. In a comparative sequence analysis we detected 84 polymorphic sites. Furthermore, we detected five mitochondrial polymorphic sites. Our project provides reference data for informed marker design that can be used population- and range-wide studies.
High latitudinal ecosystems are strongly climate-related and vegetation models project a densification and northward expansion of the Siberian larch taiga into areas currently covered by Arctic tundra. The genetic diversity and dynamics of larch populations at the tundra-taiga ecotone, the transition zone from light taiga to tundra, are largely unknown due to the remoteness and difficult accessibility. The goal of our study was to use shotgun sequencing of needle-derived total DNA to assemble the complete paternally-inherited chloroplast genomes as well as maternally-inherited mitochondrial sequences of 12 individuals from the range of Larix gmelinii and 7 from the range of Larix cajanderi and identify genetic variation. The chloroplast genomes result in a circular sequence of 122,581-122,593 bp, depending on InDels and length differences of homopolymer stretches. In a comparative sequence analysis we detected 84 polymorphic sites. Furthermore, we detected five mitochondrial polymorphic sites. Our project provides reference data for informed marker design that can be used population- and range-wide studies.