E-MTAB-9321
Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/ß-catenin signaling at the early wound healing stage
Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing (RNA-Seq) is a high-throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA-Seq technologies and their extensive data-analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study, we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing (20 hour post lesion) and early proliferative (3 day post lesion) stages of regeneration. Moreover, we uncover Wnt/ß-catenin signaling as a key pathway that is activated at the early wound healing stage.
Adult zebrafish can completely regenerate a wide range of injured organs including the CNS. RNA sequencing (RNA-Seq) is a high-throughput sequencing method facilitating quantification of gene expression in a precisely manner. Development of RNA-Seq technologies and their extensive data-analysis methods make investigation of regulatory genes and functional gene annotations possible under specific conditions. In this study, we reveal the whole transcriptome profiles of both the lesioned and unlesioned hemispheres of the zebrafish telencephalon at early wound healing (20 hour post lesion) and early proliferative (3 day post lesion) stages of regeneration. Moreover, we uncover Wnt/ß-catenin signaling as a key pathway that is activated at the early wound healing stage.