E-MTAB-4632
Expression RNA-Seq data of matched cases of pre-treatment Fine-needle aspiration (FNA) and the respective post neo-adjuvant treatment operative sample of breast cancer patients.
"Neoadjuvant treated patients were selected from the \""Genomstudy\"" of the University Women`s Clinic Heidelberg (2009-2016). This study includes primary breast cancer cases that were newly diagnosed at the University women's clinic of Heidelberg and had given their informed consent for participating in this study. This study was approved by the Ethical Committee of the Medical Faculty in Heidelberg.  All cases were female and Caucasian.  Fresh core needle biopsies and fresh tumor tissue were examined by a pathologist, snap-frozen in liquid nitrogen and stored at -80°C within 15 min after sampling.   Total RNA as well as DNA and protein were extracted by applying the QIAGEN All Prep Kit. All eluates were stored at -80°C until usage.  A sample swap most probably occurred for patient 207, so 207-S is actually in the T condition and 207-T in the S condition."
"Neoadjuvant treated patients were selected from the \""Genomstudy\"" of the University Women`s Clinic Heidelberg (2009-2016). This study includes primary breast cancer cases that were newly diagnosed at the University women's clinic of Heidelberg and had given their informed consent for participating in this study. This study was approved by the Ethical Committee of the Medical Faculty in Heidelberg.  All cases were female and Caucasian.  Fresh core needle biopsies and fresh tumor tissue were examined by a pathologist, snap-frozen in liquid nitrogen and stored at -80°C within 15 min after sampling.   Total RNA as well as DNA and protein were extracted by applying the QIAGEN All Prep Kit. All eluates were stored at -80°C until usage.  A sample swap most probably occurred for patient 207, so 207-S is actually in the T condition and 207-T in the S condition."