PRJNA836271
Phage transcipts targeting by Type III CRISPR-Cas system in Thermus thermophilus
The study is connected with the investigation of nucleic acids degradation by Type III CRISPR-Cas systems. The aim of this experiments is to identify the products of RNA degradation by Type III CRISPR-Cas systems in T. thermophilus.T. thermophilus HB8 cells harboring pMK18 plasmid or pMK18-based vector encoding mini-CRISPR arrays targeting P23-45 transcripts, were infected with P23-45 phage at high MOI. Samples of the cultures were collected before the infection (time 0), at 5 minutes post infection (time 5), at 20 and 40 minutes post infection (times 20 and 40). Total RNA samples were extracted from the cells, and buld RNA sequencing was performed.