GSE112997
Temporal profiling of redox-dependent heterogeneity in single cells
The cellular redox status plays an essential role in diverse cellular functions,  including proliferation, protein homeostasis, and aging. Thus even cells with  identical genetic background but different redox status behave differently. Here,  we propose a non-laborious and robust methodology to quantify cell-to- cell  redox-dependent variability within thousands of cells, detect oxidation levels in  cytosol, mitochondria, and peroxisome, and sort cells based on their redox  status.  We report that cells of the same age have a bi-modular distribution of reduced  and oxidized cells starting in the late logarithmic phase. Although the ratio  between the oxidized and reduced subpopulations changes during chronological  aging, their growth, proteomic and transcriptomic features are conserved during  their first days of chronological aging. A comparative proteomic analysis  identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal  oxidation levels and may serve as first line of defense proteins in redox  homeostasis. Overall design: The dataset includes 12 3''RNA-Seq samples.