PRJNA722311
A novel bioluminescent herpes simplex 1 virus for in vivo monitoring of herpes simplex encephalitis
The development of new approaches to evaluate therapeutic strategies remains crucial to improve the outcome of herpes simplex encephalitis. We report on the development of a recombinant neurovirulent HSV-1 created by inserting two reporter genes coding for tdTomato fluorescent protein and Gaussia Luciferase (GLuc) enzyme, into the UL26-UL27 intergenic region of H25 clinical strain, via Cas9-gRNA-induced homology-directed recombination strategy