ena-STUDY-NIOB-09-01-2015-15:29:23:431-304
Amplicon-based NGS sequencing of CRISPR/Cas9 off-target sites
The APC, KRAS, P53 and SMAD4 genes were mutated in human small intestinal stem cells and colon stem cells, which grow in vitro as epithelial organoids. Here we assessed candidate off-target sites for the used gRNAs in the different organoid cultures.
The APC, KRAS, P53 and SMAD4 genes were mutated in human small intestinal stem cells and colon stem cells, which grow in vitro as epithelial organoids. Here we assessed candidate off-target sites for the used gRNAs in the different organoid cultures.