PRJNA869084
Prairie dog microbiome
DNA was isolated from fresh feces (150 mg) using Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA, USA). The 16S rRNA gene in fecal samples of black-tailed prairie dogs' DNA extracts was PCR amplified with universal primers (28F-519R) targeting the V1-V3 regions. Libraries were pooled equimolar and sequenced for paired 300 bp reads at 10 pM using Illumina MiSeq reagent kit V3 (Illumina, Inc. San Diego, CA, USA) at Texas Tech Genomic Core Facility.