PRJNA704381
Sequencing of RNAs co-purifying with the type IV-B CRISPR-Cas complex from Mycobacterium sp. JS623
Type IV-B CRISPR-Cas genes were expressed together with the type I-E Cas6 and its associated CRISPR-array (all native genes from Mycobacterium sp. JS623) in E.coli BL21 (DE3) cells. The type IV-B complex was affinity purified and co-purifying RNAs were sent for sequencing.