XRNAX-CLIP-seq_on_MCF7_cells_for_LMNB1_and_EXOSC2_expid_645
"XRNAX-CLIP-seq on MCF7 cells for LMNB1 and EXOSC2"
MCF7 cells were crosslinked with 0.2 J/cm^2 UV254nm and extracted with XRNAX. XRNAX extracts were fragmented by ultrasonication and subjected to a CLIP-seq variation using a size-matched input control (SMI). Library preparation from purified RNA fragments occurred with the NextFlex Small RNA 3.0 kit (Bioo Scientific), which uses unique molecular identifiers (UMIs). For LMNB1 XRNAX-CLIP-seq was preformed on untreated MCF7 cells. For EXOSC2 XRNAX-CLIP-seq was preformed on untreated MCF7 cells and cells treated with sodium arsenite for 5 and 30 minutes.
MCF7 cells were crosslinked with 0.2 J/cm^2 UV254nm and extracted with XRNAX. XRNAX extracts were fragmented by ultrasonication and subjected to a CLIP-seq variation using a size-matched input control (SMI). Library preparation from purified RNA fragments occurred with the NextFlex Small RNA 3.0 kit (Bioo Scientific), which uses unique molecular identifiers (UMIs). For LMNB1 XRNAX-CLIP-seq was preformed on untreated MCF7 cells. For EXOSC2 XRNAX-CLIP-seq was preformed on untreated MCF7 cells and cells treated with sodium arsenite for 5 and 30 minutes.