ena-STUDY-RU-28-10-2013-13:59:55:684-7
Sequencing, assembly and annotation of an anaerobic ammonium oxidizing (anammox) enrichment culture dominated by candidatus Brocadia fulgida.
Candidatus Brocadia fulgida is a bacterium capable of anaerobic oxidation of ammonium. We have sequenced an enrichment culture obtained in a membrane bioreactor (Van der Star, 2008) consisting for over 90% of candidatus Brocadia fulgida. Sequencing was done using an IonTorrent PGM with a 200bp protocol (life technologies).  The 5250695 reads were trimmed and assembled using the CLC genomics workbench (CLCbio) and 411 contigs (>500bp) were extracted based on coverage. These 411 contigs were annotated using prokka v1.7.2 (VBC)  and curated manually   --- van der Star, Wouter RL, et al. "The membrane bioreactor: a novel tool to grow anammox bacteria as free cells." Biotechnology and bioengineering 101.2 (2008): 286-294.   --- Prokka: Prokaryotic Genome Annotation System - http://vicbioinformatics.com/
Candidatus Brocadia fulgida is a bacterium capable of anaerobic oxidation of ammonium. We have sequenced an enrichment culture obtained in a membrane bioreactor (Van der Star, 2008) consisting for over 90% of candidatus Brocadia fulgida. Sequencing was done using an IonTorrent PGM with a 200bp protocol (life technologies).  The 5250695 reads were trimmed and assembled using the CLC genomics workbench (CLCbio) and 411 contigs (>500bp) were extracted based on coverage. These 411 contigs were annotated using prokka v1.7.2 (VBC)  and curated manually   --- van der Star, Wouter RL, et al. "The membrane bioreactor: a novel tool to grow anammox bacteria as free cells." Biotechnology and bioengineering 101.2 (2008): 286-294.   --- Prokka: Prokaryotic Genome Annotation System - http://vicbioinformatics.com/