Transcriptome sequencing of rice in response to chitosan treatment
The study aims to characterize the transcriptomic response of rice (Oryza sativa L. cv. Nipponbare) to chitosan treatment. Rice seedlings received either chitosan solution or water (mock control), with samples collected at 0 h (pre-treatment baseline) and 48 h post-treatment. RNA-sequencing (RNA-seq) was used to analyze global gene expression changes between the chitosan-treated and mock-treated groups at both time points. The main objectives include identifying differentially expressed genes and signaling pathways involved in chitosan-induced defense responses against pathogens, as well as revealing the molecular mechanisms behind its potential growth-regulatory effects. This research offers insights into using chitosan as an eco-friendly biostimulant for sustainable rice production. The transcriptome data generated will be a valuable resource for functional genomics and breeding research in rice.