The effect of nicotine on gut microbiota and blood metabolism in Parkinson's rats
Epidemiological surveys have shown that smokers have a reduced probability of developing PD, and the nicotine is an important part of it, so it is important to study the mechanism of action of nicotine for PD. In this study, rotenone was used to establish Parkinson's disease (PD) model in different gender of rats, and then nicotine was administered. The behavioral changes of PD rats were evaluated by the CatWalk systemgait and rod rotation, the pathological changes were detected, and the therapeutic effects of nicotine on PD rats were studied by combining intestinal bacteria and metabolomics. The results showed that nicotine could effectively improve the expression of motor injury and neuronal injury in PD rats, and reduce the levels of proinflammatory cytokines in blood and colon, and inhibit neuroinflammation. And tThere was no difference between male and female rats. In addition, nicotine significantly changed the abundance of intestinal bacteria and metabolites of different biological pathways in male and female PD ratsmice, and the changes were quite different between female and male ratsmice. And there is a significant correlation between serum metabolite changes and gut microbiota. Taken together, these findings suggest that regulation of metabolism and intestinal microbiota composition and function may be important mechanisms of nicotine therapy in PD, and the mechanisms are different in male and female rats.