Co-application of organic amendments and encapsulated plant growth promoting fungi enhance growth of KDML 105 rice and microbial community structure in the rhizosphere under salinity stress
The rhizosphere soil pellet (0.5 g) was transferred in the column of a Quick-DNATM Fecal/Soil microbe miniprep kit (Zymoresearch, UK). The full-length 16S rRNA gene in bacteria were amplified with primers (27F: AGRGTTTGATYNTGGCTCAG and 1492R: TASGGHTACCTTGTTASGACTT). The internal transcribed spacer (ITS) regions in fungi were amplified with primers (F:CTTGGTCATTTAGAGGAAGTAA and R:TCCTCCGCTTATTGATATGC). PacBio barcodes were added for multiplexing.