A chromosome-level genome assembly of R. dybowskii
A chromosome-level genome of R. dybowskii was constructed by integrating PacBio HiFi long-read sequencing for de novo assembly and CiFi (3C coupled with HiFi sequencing) for scaffolding. The final assembly comprises 12 chromosomes, spanning 3.95 Gb in total, with a scaffold N50 of 455 Mb. BUSCO assessment against the tetrapoda_odb12 database revealed 94.2% complete and 0.5% fragmented orthologs.
A chromosome-level genome of R. dybowskii was constructed by integrating PacBio HiFi long-read sequencing for de novo assembly and CiFi (3C coupled with HiFi sequencing) for scaffolding. The final assembly comprises 12 chromosomes, spanning 3.95 Gb in total, with a scaffold N50 of 455 Mb. BUSCO assessment against the tetrapoda_odb12 database revealed 94.2% complete and 0.5% fragmented orthologs.