Virus discovery and antiviral RNAi response in Bactrocera tryoni laboratory populations
Stranded transcriptome and small RNA sequencing were performed in Bactrocera tryoni laboratory populations to comprehensively characterise the virome and RNAi-based immune response to these viruses. Eight pooled samples were sequenced from five populations: population C28 and LE where ovaries and somatic tissue were sequenced separately, C58 that had been injected with buffer or virus suspension derived from C28, and populations B6 and B9. B. tryoni is a major horticultural pest and understanding the virome and immune response may improve pest management strategies.