RNA sequencing upon CD146 knockdown/overexpression
RNA sequencing analysis was performed on liver cancer cells upon genetic manipulation of their CD146 levels. For these assays, total RNA samples were prepared using TRIzol reagent from Hep3B cells infected with lentiviruses expressing Tet-Control shRNA or Tet-CD146 shRNA, and HepG2 cells infected with lentiviruses expressing pLVX or CD146oe. The library and transcriptome sequencing were analyzed by AZENTA Life Sciences (Suzhou, China). Gene set enrichment analysis was applied to analyze the gene expression signatures using the Gene Ontology, Hallmark, and KEGG gene sets.