Testing the waters: Comparison of methods to assess coastal zooplankton biodiversity
We sampled 25 shallow sites along an estuarine gradient in the northwestern Gulf of Finland to assess zooplankton biodiversity. Samples were taken either using a 50µm plankton net or a 2 L limnos water sampler. Net samples were used for bulk sample metabarcoding, water samples were used for eDNA metabarcoding. All metabarcoding was done targeting COI using the forward primer mlCOIintF and the reverse primer HCO2198. Further net samples were analysed using microscopy and automated imaging to provide a comparison between methods.
We sampled 25 shallow sites along an estuarine gradient in the northwestern Gulf of Finland to assess zooplankton biodiversity. Samples were taken either using a 50µm plankton net or a 2 L limnos water sampler. Net samples were used for bulk sample metabarcoding, water samples were used for eDNA metabarcoding. All metabarcoding was done targeting COI using the forward primer mlCOIintF and the reverse primer HCO2198. Further net samples were analysed using microscopy and automated imaging to provide a comparison between methods.