Composition of fungal communities in forest soils across Yunnan, China
Soil samples (0-10 cm depth) from seven forest types across Yunnan Province, China were collected to analyze the fungal community and determine soil chemistry. Mid-infrared diffuse spectroscopy (MIR) was used to determine soil chemical properties. The reference dataset for the MIR was comprised of a third of the soil samples using the following protocols: pH, NY/T 1377-2007; SOC, NY/T 1121.6-2006; TN, NY/T 53-1987; TP, NY/T 88-1988; TK, NY/T 87-1988. Climate data, including temperature and precipitation (from 1970-2000), were taken from the WorldClim database. The forest types include: tropical, monsoon evergreen broad-leaved, mountainous humid evergreen broad-leaved, semi-humid evergreen broad-leaved, temperate coniferous, warm-hot coniferous, and warm-temperate coniferous. DNA was extracted from the soil samples and amplicon libraries were constructed by amplifying the fungal ITS2 barcoding region in two consecutive PCRs using the gITS7 and ITS4g primers. Sequencing was performed using an Illumina MiSeq sequencer.
Soil samples (0-10 cm depth) from seven forest types across Yunnan Province, China were collected to analyze the fungal community and determine soil chemistry. Mid-infrared diffuse spectroscopy (MIR) was used to determine soil chemical properties. The reference dataset for the MIR was comprised of a third of the soil samples using the following protocols: pH, NY/T 1377-2007; SOC, NY/T 1121.6-2006; TN, NY/T 53-1987; TP, NY/T 88-1988; TK, NY/T 87-1988. Climate data, including temperature and precipitation (from 1970-2000), were taken from the WorldClim database. The forest types include: tropical, monsoon evergreen broad-leaved, mountainous humid evergreen broad-leaved, semi-humid evergreen broad-leaved, temperate coniferous, warm-hot coniferous, and warm-temperate coniferous. DNA was extracted from the soil samples and amplicon libraries were constructed by amplifying the fungal ITS2 barcoding region in two consecutive PCRs using the gITS7 and ITS4g primers. Sequencing was performed using an Illumina MiSeq sequencer.