Comparative translocation profiling between on-target sites of Cas9 nuclease- and adenine base-edited multiplex knockout CAR T cells
Targeted DNA sequencing to quantify on-target editing efficiency and to complementarily estimate chromosomal translocations between on-target sites in primary human CAR T cells following multiplex genome editing. Uses a custom hybrid-capture panel (Twist Biosciences) spanning candidate on-target editing sites, comparing CRISPR-Cas9 nuclease editing to adenine base editing (ABE) at matched 10-plex and 20-plex multiplex knockout levels, alongside mock-edited controls, in CAR T cells from two donors.