Salt Stress-Driven Goitrin Accumulation in Isatis indigotica Roots via Glucosinolate Pathway Regulation
This project investigates the transcriptomic response of Isatis indigotica Fort. (Inner Mongolia accession) roots to NaCl stress. Seedlings were treated with 100 mmol/L NaCl for four time points (2, 6, 12, and 24 h), with distilled water as control. Total RNA was extracted from roots and subjected to strand-specific RNA-seq using the Illumina NovaSeq platform. The study aims to identify differentially expressed genes and key regulatory networks associated with the biosynthesis of (R,S)-goitrin, the primary quality marker of I. indigotica roots, under salt stress conditions. A total of 24 libraries were constructed from 4 time points x 2 treatments x 3 biological replicates.