Hepatic RNA-seq analysis of vaccenic acid-treated diet-induced obese mice reveals LRH-1/NR5A2-regulated metabolic pathways
RNA-seq analysis was performed on liver tissue from high-fat diet-induced obese mice treated with vaccenic acid (VA) or vehicle control to investigate transcriptional mechanisms underlying VA-mediated effects. Sequencing libraries were generated using the TruSeq Stranded mRNA protocol and sequenced on an Illumina NextSeq500 platform. Differential gene expression analysis identified hepatic pathways associated with LRH-1/NR5A2 signaling and suppression of de novo lipogenesis.