SHIV-325C Viral Stock Deep Sequencing
Laboratory viral stock of simian-human immunodeficiency virus was deep sequenced to determine the frequency of single nucleotide polymorphisms. Macaques given prophylactic PGDM1400 were challenged with 500 MID50 SHIV-325C (sensitive) and 0.5 MID50 SHIV-SF162P3 (resistant), and certain animals became infected with SHIV-325C variants found to be resistant to PGDM1400. These variants shared the S380H (relative to SHIV-325C) mutation, and each animal had an additional mutation in their cloned viruses that varied per animal. Thus, we sought to determine at what frequency these mutations were present in our viral stock of SHIV-325C.