Long-term nitrogen fertilization shapes plant-microbe interactions across soil, rhizosphere, and nodules
Full length 16S rRNA and nodA sequencing of soil and plant material from multiple compartments: source (field) soil, greenhouse (bulk) soil, rhizosphere, and nodules undergoing long-term N-fertilization. This study aims to evaluate ASV-level response to environmental and spatial change across scales from the whole bacterial community, to individual populations of microbes, to species-level and plasmid-level dynamics.