Off-target profiling of high-multiplex adenine base-edited T cells
Off-target safety assessment of a 20-plex adenine base editing (ABE) knockout strategy for T cell engineering. Includes genome-wide off-target nomination via UNCOVERseq in HEK293-Cas9 cells (tag-only background control and pooled 20-guide RNA nomination) and targeted validation of nominated candidate sites via rhAmpSeq in primary human T cells across increasing multiplex levels (5x-20x knockout) and mock-edited controls, from three healthy donors