DLP+ single-cell whole-genome sequencing of COLO320DM cells following FANCM knockout or DNA polymerase theta inhibition to assess extrachromosomal DNA structural variation
This BioProject contains DLP+ single-cell whole-genome sequencing data from the human COLO320DM colorectal cancer cell line, which harbors MYC-amplified extrachromosomal DNA (ecDNA). Four conditions were analyzed: parental cells at baseline (T=0), DMSO-treated control cells cultured for 2 months, cells subjected to CRISPR/Cas9-mediated FANCM knockout and subsequently cultured for 1 month, and cells treated for 2 months with a DNA polymerase theta helicase-domain inhibitor. The study examines how FANCM disruption and DNA polymerase theta inhibition affect ecDNA structural variation. These data were used to identify ecDNA-associated deletions, duplications, inversions, and other structural variants and to assess their association with TA-rich fragile regions.