Exploring bacterial community structure shift and screening for potential PE degrading strains after long exposure to PE plastic films
To Acclimatize bacteria community toward PE biodegradation, around 3 grams of soil from each sample was incubated in a medium composed of 0.9% natural salt solution and PE film strips (LDPE and HDPE) obtained from a local factory. Two batches have been for each sample, one allowing air exchange and the other does not allow air exchange. The former was incubated for 4 weeks, and the latter was incubated for 8 weeks. After incubation the around 3 ml of the culture was centrifuged and prepared for DNA extraction and metabarcoding sequencing. Based on growth on PEG 6000 as sole carbon source in a mineral medium 38 identified strains were isolated, and later the effect of strain exposure to LDPE films, 11 strains out of the 38 were selected for genome sequencing. The aim of this sequence is to explore genes potentially involved in LDPE biodegradation stages and other genes that can boost the bioremediation role of these strains.
To Acclimatize bacteria community toward PE biodegradation, around 3 grams of soil from each sample was incubated in a medium composed of 0.9% natural salt solution and PE film strips (LDPE and HDPE) obtained from a local factory. Two batches have been for each sample, one allowing air exchange and the other does not allow air exchange. The former was incubated for 4 weeks, and the latter was incubated for 8 weeks. After incubation the around 3 ml of the culture was centrifuged and prepared for DNA extraction and metabarcoding sequencing. Based on growth on PEG 6000 as sole carbon source in a mineral medium 38 identified strains were isolated, and later the effect of strain exposure to LDPE films, 11 strains out of the 38 were selected for genome sequencing. The aim of this sequence is to explore genes potentially involved in LDPE biodegradation stages and other genes that can boost the bioremediation role of these strains.