Transcriptome profiling of the salt-stress response in Arabidopsis thaliana
This project generates a transcriptome profile of the salt-stress response in Arabidopsis thaliana, including the ecotype Columbia-0 (Col-0) as control and two SALK T-DNA insertion mutants (SALK_073282 and SALK_121298). The salt treatment was conducted as follows. After 2-day stratification in the dark at 4Â°C, the sealed plates with seeds were transferred to a plant culture room at 22 Â°C under 16/8 h light/dark photoperiod for 7 days. From the 8th day, the cultivated seedlings were transferred to the standard growth media (as control) and medium prepared with the addition of NaCl at concentrations of 75 mM. On the 15th day, culture plates were photographed for the determination of the 7-day root growth and the root were immediately snap-frozen in liquid nitrogen and stored in -80Â°C prior to RNA extraction.