ZJ_River_Site Raw sequence reads
This project investigates the potential of environmental DNA (eDNA) metabarcoding as a tool for assessing freshwater ecosystems. River water samples collected throughout Zhejiang Province, China, were analyzed using high-throughput amplicon sequencing to describe aquatic community composition and examine its relationship with conventional biological and water-quality indicators. The study develops and tests generalizable, data-oriented approaches that can function effectively despite incomplete taxonomic reference libraries, enhancing the reliability and broad applicability of eDNA monitoring. The generated sequencing data and accompanying metadata offer a useful foundation for methodological advances in molecular ecology and for reproducible biomonitoring across freshwater environments.