Genetic diversity and population structure of wild Ribes populations (Ribes nigrum, Ribes rubrum and Ribes uva-crispa) in the Netherlands assessed using MobiSeq-based genotyping
In total,  542 individuals of three Ribes species (R. nigrum, R. rubrum and R. uva-crispa) were sampled from an ex situ genebank collection and from in situ populations in the Netherlands, alongside 43 well-known cultivars of the three Ribes species. Using the primer AG_MobiRibes_78 (5' GACTCCGGATTTGGGTCGTTAC 3') a MobiSeq library was generated for each of the samples (following Rey-Iglesia et al. (2019), Mol. Ecol. Resources 19, 512–525; https://doi.org/10.1111/1755-0998.12984), which were then bulked and sequenced in an Illumina NovaSeq PE150 flow cell. Library preparation was carried out by AllGenetics & Biology SL (www.allgenetics.eu).
In total,  542 individuals of three Ribes species (R. nigrum, R. rubrum and R. uva-crispa) were sampled from an ex situ genebank collection and from in situ populations in the Netherlands, alongside 43 well-known cultivars of the three Ribes species. Using the primer AG_MobiRibes_78 (5' GACTCCGGATTTGGGTCGTTAC 3') a MobiSeq library was generated for each of the samples (following Rey-Iglesia et al. (2019), Mol. Ecol. Resources 19, 512–525; https://doi.org/10.1111/1755-0998.12984), which were then bulked and sequenced in an Illumina NovaSeq PE150 flow cell. Library preparation was carried out by AllGenetics & Biology SL (www.allgenetics.eu).