Optimization of a new method to remove humic substances during soil DNA extraction
Soil samples are frequently subject to DNA extraction and molecular exploration from simple binary PCRs of presence or absence to sequencing using short read sequencing by synthesis methods (Sanger, pyrosequencing, Illumina, MGI) and long read sequencing (Nanopore and PacBio). The quality of the results depends heavily on the quality of the extract, whether it is in high molecular weight or not, and whether it has inhibitors, especially for PCR amplifications. Humic substances are famous PCR inhibitors that are very abundant in soil samples, removing them during total DNA extraction has been a challenge for a long time, in this study we are exploring a new method that combines literature data and experiment design to optimize a new DNA extraction method for high purity high molecular weight DNA from humic substances rich soil samples.
Soil samples are frequently subject to DNA extraction and molecular exploration from simple binary PCRs of presence or absence to sequencing using short read sequencing by synthesis methods (Sanger, pyrosequencing, Illumina, MGI) and long read sequencing (Nanopore and PacBio). The quality of the results depends heavily on the quality of the extract, whether it is in high molecular weight or not, and whether it has inhibitors, especially for PCR amplifications. Humic substances are famous PCR inhibitors that are very abundant in soil samples, removing them during total DNA extraction has been a challenge for a long time, in this study we are exploring a new method that combines literature data and experiment design to optimize a new DNA extraction method for high purity high molecular weight DNA from humic substances rich soil samples.