ONT sequencing of GNI2-associated homoeo-alleles in wheat
We investigated homoeologous loci associated with the GRAIN NUMBER INCREASE 2 (GNI2) gene in tetraploid and hexaploid wheat. Using Oxford Nanopore Technologies (ONT) sequencing, we generated long-read amplicon sequencing data to characterize sequence variation across GNI-A2, GNI-B2, and GNI-D2 loci. For hexaploid wheat, ONT reads were aligned to the wheat cultivar Fielder reference genome, and reads corresponding to GNI loci were extracted prior to FASTQ generation and archival submission. For tetraploid wheat, FASTQ files corresponding to both GNI1 and GNI2 loci were generated directly from basecalled FAST5 files. Sequencing datasets include reads corresponding to both GNI1 and GNI2 loci, although the present study primarily focuses on GNI2-associated variation.
We investigated homoeologous loci associated with the GRAIN NUMBER INCREASE 2 (GNI2) gene in tetraploid and hexaploid wheat. Using Oxford Nanopore Technologies (ONT) sequencing, we generated long-read amplicon sequencing data to characterize sequence variation across GNI-A2, GNI-B2, and GNI-D2 loci. For hexaploid wheat, ONT reads were aligned to the wheat cultivar Fielder reference genome, and reads corresponding to GNI loci were extracted prior to FASTQ generation and archival submission. For tetraploid wheat, FASTQ files corresponding to both GNI1 and GNI2 loci were generated directly from basecalled FAST5 files. Sequencing datasets include reads corresponding to both GNI1 and GNI2 loci, although the present study primarily focuses on GNI2-associated variation.