16S rRNA gene sequences from bentonite cores from the Tsukinuno mine
16S rRNA gene sequences from bentonite cores taken from the Tsukinuno mine (Japan) and corresponding cultures inoculated with each clay sample. Three cores (A, B, C) were taken from the D4 site and one from the D6 site. Depth is in meters refers to the distance from the tunnel wall (e.g., a depth of 0 is exposed bentonite). From each core, bentonite was collected in triplicate (R1, R2, R3) for DNA extraction and sequencing at each 1 m mark. Each bentonite sample was also used to inoculate cultures of aerobic and anaerobic heterotrophs (growth on R2A agar) and sulfate-reducing bacteria (SRB) grown in a liquid SRB medium. All cultures were incubated at 30 degrees C. Biomass was collected for DNA extraction and sequencing from all cultures with growth. Two types of water samples are also included: groundwater from the mine and the tap water used for core drilling. All kit controls with "PM" in the sample name refer to PowerMax (Qiagen) extractions, which were used for all clay samples. All kit controls with "UC" in the sample name refer to UltraClean (Qiagen) extractions, which were used for all culture samples.
16S rRNA gene sequences from bentonite cores taken from the Tsukinuno mine (Japan) and corresponding cultures inoculated with each clay sample. Three cores (A, B, C) were taken from the D4 site and one from the D6 site. Depth is in meters refers to the distance from the tunnel wall (e.g., a depth of 0 is exposed bentonite). From each core, bentonite was collected in triplicate (R1, R2, R3) for DNA extraction and sequencing at each 1 m mark. Each bentonite sample was also used to inoculate cultures of aerobic and anaerobic heterotrophs (growth on R2A agar) and sulfate-reducing bacteria (SRB) grown in a liquid SRB medium. All cultures were incubated at 30 degrees C. Biomass was collected for DNA extraction and sequencing from all cultures with growth. Two types of water samples are also included: groundwater from the mine and the tap water used for core drilling. All kit controls with "PM" in the sample name refer to PowerMax (Qiagen) extractions, which were used for all clay samples. All kit controls with "UC" in the sample name refer to UltraClean (Qiagen) extractions, which were used for all culture samples.