To investigate the transcriptional regulation in the BTPC-edited Solanum lycopersicum var. Moneymaker fruits
The current study generated CRISPR-Cas9-edited Solanum lycopersicum var. Moneymaker lines with targeted disruption of the Bacterial-type Phosphoenolpyruvate Carboxylase (BTPC) gene. Total RNA samples were extracted from three BTPC-edited lines (so-called L7, L8, L13) and wild type (WT) plant with three biological replicates per sample, from pericarp tissue at the mature green stage. Samples were subjected to the strand-specific RNA library preparation using polyA-selection and 2x150bp sequencing on an Illumina Nova-Seq platform (>20M paired-end mRNA reads).  These data were used to investigate the transcriptional regulation in the BTPC-edited fruits.
The current study generated CRISPR-Cas9-edited Solanum lycopersicum var. Moneymaker lines with targeted disruption of the Bacterial-type Phosphoenolpyruvate Carboxylase (BTPC) gene. Total RNA samples were extracted from three BTPC-edited lines (so-called L7, L8, L13) and wild type (WT) plant with three biological replicates per sample, from pericarp tissue at the mature green stage. Samples were subjected to the strand-specific RNA library preparation using polyA-selection and 2x150bp sequencing on an Illumina Nova-Seq platform (>20M paired-end mRNA reads).  These data were used to investigate the transcriptional regulation in the BTPC-edited fruits.