Drosophila melanogaster longituidnal pigmentation mapping
Raw sequencing reads generated via a tail-based mapping of abdominal pigmentation in large genetically diverse populations evolving in both outdoor (field) and indoor (lab) mesocosms. Tail-based mapping was conducted via a selective isolation of the 15% lightest and 15% darkest flies and a representative sample of the trait midpoint (N = 75 individuals per trait value group) within each of 10 replicate populations at two time points in each of the two evolution environments. The file names follow the format TP[2/8].[E/I][#].[L/M/D].R[1/2].fastq.gz. The numeric following 'TP' indicates the time point at which the sample was collected - time point 2 refers to the first mapping time point (4 weeks into the experiment) and time point 8, the second time point (10 weeks into the experiment). 'E' or 'I' indicates whether the sample was derived from the outdoor or indoor mesocosms, respectively. The numeric following the mesocosm location indicates the replicate cage number. 'L', 'M', 'D' then refers to the trait value group from which the sample is derived - light, midpoint, or dark, respectively. Further methodological details can be found here - 10.1101/2025.11.04.686622