Transcriptional profile of the CAL-27 cell line treated with chiral extracellular matrices
To exclude a global transcriptional bias caused by the material itself, RNA seq was performed using a non-differentiating tumor cell line CAL 27 cultured on the two chiral substrates and differentially expressed genes were defined as fold change larger than 2 and FDR less than 0.05. In contrast to MSCs, fewer differentially expressed genes and no transcriptional bias were observed between two gene sets, suggesting that the asymmetric transcriptional responses are associated with lineage-specific differentiation rather than nonspecific material stimulation.