16S rRNA sequencing analysis of gut microbiota modulation by walnut and Psoralea corylifolia combination in depression like zebrafish
This project investigates the effects of a walnut and Psoralea corylifolia combination on the gut microbiota of depression like zebrafish based on the brain gut axis theory. Using 16S rRNA amplicon sequencing, the study evaluates changes in microbial community composition, alpha diversity, beta diversity, dominant bacterial taxa, and predicted functional pathways after treatment. The results are intended to clarify the association between gut microbial alterations and behavioral phenotypes and to provide microbiological evidence for the potential brain gut regulatory effects of this traditional Chinese medicine combination.