Plasmodium falciparum: 3 stages, nanopore, plasmidsaurus and Dart-Seq
Plasmodium falciparum is an obligate human parasite of the phylum apicomplexa and is the causative agent of the most lethal form of human malaria. While post-transcriptional regulatory mechanisms are critical for expression of stage-specific virulence factor proteins, the mechanisms underlying these processes are yet to be uncovered in apicomplexan parasites. Most eukaryotic mRNA precursors (pre-mRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3 primed end. Particularly, 3 primed end processing promotes mRNA stability, transport and translation. In this study, we provide a comprehensive landscape of 3 stages using nanopore, Illumina and paired end Dart-Seq