A Circulating Tumor Cell-Based Study Reveals LINC00092/miR-373-5p/SOCS2 Axis as a Key Regulator of Osteosarcoma Progression and Metastasis
Total RNA was extracted from CTC-derived organoid models successfully established from 3 patients with metastatic osteosarcoma (OS) and from peripheral blood samples from 3 healthy volunteers used as controls. The quality and integrity of the extracted RNA were assessed using the Agilent Bioanalyzer 2100 system. Paired-end sequencing was performed on the Illumina HiSeq platform.