Homo sapiens isolate:IOMM-Lee Genome sequencing and assembly
Background: Meningiomas are the most common primary nervous system neoplasm in adults. There are few good cellular models, especially for high grade/malignant meningiomas, despite the continuing need to identify new therapeutic agents and treatment regimens. Meningioma cell line disease models provide one way to meet this need, with NF2+/wildtype IOMM-Lee a readily available, partially characterized Grade 3/malignant meningioma cell line model.Methods: We generated new IOMM-Lee genomic and mtDNA variant data, determined proliferation rate, colony-forming efficiency, and sensitivity to ionizing radiation prior to and after ATM kinase inhibition. A high throughput screen using a 349 anti-cancer drug library to identify mechanistically different, clinically useful drugs with nanomolar-range IC50 values.Results: Key cellular, molecular and drug response profiling results from the above analyses better place IOMM-Lee on the landscape of genomically-defined meningiomas and corresponding cell line models.Summary: Our results will further inform and enable use of this experimentally tractable malignant meningioma cell line to understand meningioma biology, and to identify new therapies and more effective treatment regimens.