A Comparison between Passive airDNA Collection and Extraction Methods on the Recovered Terrestrial Vertebrate Assemblage
SRA data for the publication titled above. Sequencing files are from an Illumina Novaseq X Platform using both the Mamm02 and V12S-U primers, where first letter outlines each kit respectively (i.e., M_X_X_01, V_X_X_01). Four extraction kits are used, Blood &amp;amp; Tissue, MagMAX, Powersoil and Quick-DNA Miniprep Plus (Zymo), outlined by the second letter (i.e., X_B_X_01, X_M_X_01, X_P_X_01, X_Z_X_01). Two filter types are used, medical gauze (cloth) and paraffin gauze (i.e., X_X_C_01, X_X_P_01). The unique filter number is then outlined by the number (i.e., X_X_X_01). All files are processed to remove human reads, first using vsearch, with settings allowing 97% minimum similarity between the forward and reverse sequences, a maximum of 5bp difference and minimum overlap length of 16 basepairs, following which the reads are mapped to the human genome and removed. Files are then rezipped and presented here. Detailed explanation for the removal of human reads is outlined within the titled publication.