Genome-wide transposon sequencing identifies genes required for intestinal adaptation of Klebsiella pneumoniae
This project investigates the genetic determinants required for intestinal colonization and environmental adaptation of Klebsiella pneumoniae. A genome-wide Himar1 transposon insertion sequencing (Tn-seq) library was constructed in K. pneumoniae ATCC 43816 (KPPR1), containing more than 200,000 independent mutants. The transposon mutant library was screened under different intestinal environments, including cecal filtrates derived from specific pathogen-free (SPF), antibiotic-treated (ABX), and germ-free (GF) mice. Comparative analysis of mutant abundance was performed to identify genes that contribute to bacterial fitness and survival under host-associated conditions. The resulting dataset provides a comprehensive resource for understanding the genetic basis of K. pneumoniae intestinal colonization.