Liver RNA-Seq in Duroc pigs
Duroc pigs from the IMMUPIGEN population (Institut de Recerca i Tecnologia Agroalimentàries, IRTA) were fed a cereal-based diet ad libitum and did not show any signs of infection. Animals were slaughtered at 181-228 days of age in a commercial slaughterhouse. Liver samples were collected immediately after slaughter using RNAlater and snap-frozen at –80ºC until extraction. RNA was extracted at Centre for Research in Agricultural Genomics (CRAG) from 210 liver samples and RNA sequencing (RNA-Seq) was done using an Ilumina 6000 S4 platform at Centro Nacional de Análisis Genómico (CNAG). Data analysis was performed at CRAG. The aim of the study was to explore genomic regions regulating the liver transcriptome and energy homeostasis in pigs. This research was financially supported by projects METAPIGEN PID2020-112677RB-C21-22 and METAPIGSEL PID2023-148961OB-C21-22 funded by MCIN/ AEI /10.13039/501100011033 and the Fondo Europeo de Desarrollo Regional (FEDER).
Duroc pigs from the IMMUPIGEN population (Institut de Recerca i Tecnologia Agroalimentàries, IRTA) were fed a cereal-based diet ad libitum and did not show any signs of infection. Animals were slaughtered at 181-228 days of age in a commercial slaughterhouse. Liver samples were collected immediately after slaughter using RNAlater and snap-frozen at –80ºC until extraction. RNA was extracted at Centre for Research in Agricultural Genomics (CRAG) from 210 liver samples and RNA sequencing (RNA-Seq) was done using an Ilumina 6000 S4 platform at Centro Nacional de Análisis Genómico (CNAG). Data analysis was performed at CRAG. The aim of the study was to explore genomic regions regulating the liver transcriptome and energy homeostasis in pigs. This research was financially supported by projects METAPIGEN PID2020-112677RB-C21-22 and METAPIGSEL PID2023-148961OB-C21-22 funded by MCIN/ AEI /10.13039/501100011033 and the Fondo Europeo de Desarrollo Regional (FEDER).