Single cell analysis of gastric cancer-derived cell line MKN-45
Spheroids, which are 3D cultures, are believed to have a large number of cancer stem cells, and their analysis can identify cancer stem cell-associated molecules. Here, we performed single-cell RNAseq analysis of MKN-45, a gastric cancer-derived cell line, in normal and spheroid culture conditions. The objective is to identify cancer stem cells in spheroids on a single-cell basis.