Original sequencing data from the fecal microbial diversity sequencing project of mus musculus
Fecal microbial diversity testing is performed to analyze gut microbiota composition, explore its links with host metabolism, immunity and diseases, and track how interventions alter microbial communities.The basic steps are as follows: First, collect fresh mouse feces and extract total microbial DNA. Second, amplify target genes (typically 16S rRNA) via PCR. Third, construct sequencing libraries and conduct high-throughput sequencing. Finally, bioinformatic analyses are carried out to assess species richness, community structure and functional differences of microbes.