Onthophagus sagittarius tissue-specific bulk ATAC-seq
Onthophagini horned beetles are an emerging model clade for the study of intra and interspecific morphological diversification. This sequencing approach focused on the development of five adult traits in the species Onthophagus sagittarius that vary in the degree of morphological sex differences they exhibit in the focal species and across closely related species: external genitalia, posterior head, anterior head, fore tibia, and elytra. The epithelially-derived tissue primordia of each trait were dissected from pupae within 12 hours of pupation and prepared for ATAC-sequencing. Five male and five female biological replicates were collected from each tissue type. Sequencing was performed on an Illumina NextSeq550 platform with a 75-cycle flow cell over four sequencing runs to generate the desired sequencing depth, generating up to four technical replicates per biological sample.