PhIP-Seq profiling of IgE epitopes in house dust mite allergens in allergic respiratory diseases
This project contains paired-end PhIP-Seq data generated from serum samples of 118 patients with house dust mite-associated allergic respiratory diseases and 50 healthy controls recruited in Wuxi and Chongqing, China. A T7 phage-displayed allergen peptide library was used to profile IgE-reactive peptides derived from house dust mite allergens. IgE-bound phages were enriched by immunoprecipitation, and the peptide-encoding inserts were amplified and sequenced on an Illumina NovaSeq 6000 platform using 150-bp paired-end reads. The study aimed to identify conserved linear IgE epitopes shared by Dermatophagoides pteronyssinus and Dermatophagoides farinae, with particular emphasis on Der p 23 and Der f 23, and to characterize the molecular basis of cross-species sensitization. Raw sequencing data from all 168 samples are included. Two Chongqing samples were excluded from downstream analysis because of insufficient assembled read depth but are retained in the archive.