Transcriptomic analysis of Phaeodactylum tricornutum reveals the influence of symbiotic bacteria on its diurnal rhythm
This study investigates the impact of symbiotic bacteria on the diurnal gene expression patterns of the diatom Phaeodactylum tricornutum. We performed a time-series RNA-Seq experiment comparing co-cultures of P. tricornutum with bacteria against an axenic heterotrophic control culture. Samples were collected every 4 hours over a 24-hour light-dark (12:12) cycle. Specifically, co-culture samples were taken on day 4 and day 8 to observe potential temporal dynamics in the symbiotic relationship. Each timepoint was sampled with three biological replicates. This dataset provides a comprehensive resource for understanding algae-bacteria interactions and their role in regulating the host's circadian clock and metabolic pathways.