Capra hircus Transcriptome or Gene expression
This study used ileal epithelial stem cells from Saanen dairy goats to construct a goat ileal organoid model. Four treatment groups were established: blank control group (Con), low inflammation stimulation group (LI), high inflammation stimulation group (HI), and high inflammation + resveratrol intervention group (HI+RSV). The culture medium for the low inflammation stimulation group was supplemented with 29 pg/mL TNF-alpha and 17 pg/mL IL-1beta, while the high inflammation stimulation group received 36 pg/mL TNF-alpha and 21 pg/mL IL-1beta. The concentrations of both inflammatory factors were determined based on ELISA-measured plasma levels of TNF-alpha and IL-1beta in healthy and diarrheic dairy goats. The HI+RSV group was treated with 10 uM resveratrol (RSV) in addition to the high inflammation stimulation, aiming to investigate the reparative and protective effects of resveratrol on inflammation-damaged goat intestinal organoids.