Single-cell RNA sequencing of immune infiltrate in WT and C1galt1c1-KO mPDAC tumors
Pancreatic ductal adenocarcinoma is characterized by a highly immunosuppressive tumor microenvironment. This study used single-cell RNA sequencing to characterize tumor-infiltrating immune cells from murine pancreatic ductal adenocarcinoma tumors with intact or disrupted C1galt1c1 expression. C1galt1c1 loss results in increased expression of truncated O-glycans, including the Tn antigen. CD45-positive immune cells were isolated from subcutaneous 2838c3 parental and isogenic 2838c3 C1galt1c1-knockout tumors grown in immunocompetent C57BL/6 mice. Single-cell transcriptomic profiling was performed to define immune cell populations and identify changes in immune composition and gene expression associated with aberrant O-glycosylation. The dataset supports analysis of myeloid-derived suppressor cells, tumor-associated macrophages, conventional dendritic cells, T-cell populations, and pathways related to antigen processing, antigen presentation, and myeloid-mediated immunosuppression.