16S rRNA amplicon sequencing of fecal microbiota from mice fed a high arachidonic acid diet with or without ferrostatin-1 treatment
Dietary arachidonic acid (AA), a polyunsaturated fatty acid enriched in the Western diet, has been linked to lipid peroxidation and ferroptosis-related tissue injury; however, its impact on the gut microbiota remains poorly understood. This study investigated the effects of a high-AA diet on the gut microbiome of male C57BL/6 mice and whether the ferroptosis inhibitor ferrostatin-1 (Fer-1) modulates these changes. Mice were assigned to three groups (n = 5 each): control (standard chow), AA model (20% AA-supplemented chow), and AA + Fer-1 (20% AA-supplemented chow plus Fer-1 at 5 mg/kg intraperitoneally every 2 days). Fecal samples were collected at baseline (before diet intervention) and at the endpoint (week 15). The V3-V4 hypervariable regions of the 16S rRNA gene were amplified with primers 341F/806R and sequenced paired-end on the DNBSEQ-T7 platform (MGI). This dataset comprises 30 samples (60 FASTQ files) and provides insights into AA-induced gut microbial dysbiosis and the potential modulatory role of ferroptosis inhibition on the gut microbiota.