Transcriptomic analysis of HUH-7 hepatocellular carcinoma cells treated with HXS protein
This project investigates the transcriptomic response of HUH-7 human hepatocellular carcinoma cells to HXS protein treatment. HUH-7 cells were treated with HXS protein (derived from Akkermansia muciniphila) or PBS vehicle control, with four biological replicates per group. Total RNA was extracted and subjected to paired-end RNA sequencing (RNA-seq). Differentially expressed genes and enriched pathways were identified to characterize the molecular mechanisms underlying HXS protein activity in liver cancer cells.