Gut microbiota 16S rRNA sequencing in shift-working medical staff and day-shift controls
This study investigated the associations between shift work, serum 25-hydroxyvitamin D levels, and gut microbiota composition in medical staff. Fecal samples were collected from 332 participants (200 shift workers and 132 day-shift controls) and subjected to 16S rRNA gene sequencing targeting the V4 hypervariable region. The raw sequence data generated in this study are archived in the NCBI Sequence Read Archive (SRA) and provide a resource for exploring the impact of occupational circadian disruption on gut microbial ecology.