Time-dependent mutagenic effects of benzo(b)fluoranthene, a polycyclic aromatic hydrocarbon, in MutaMouse lung tissue revealed by error-corrected sequencing
The genotoxic potential of benzo(b)fluoranthene (BbF) was evaluated in male MutaMouse exposed to five dose levels of BbF or vehicle control by oral gavage for 28, 90, or 180 days, with dose ranges adjusted according to exposure duration. Mutagenesis was assessed in lung tissue samples (n = 4 per dose) at each time point using Duplex Sequencing (DS), a targeted, error-corrected next-generation sequencing technology (TwinStrand Duplex Sequencing Mutagenesis Panel, Mouse-50, v2.0 kit with enzymatic fragmentation). In addition, chromosomal damage was evaluated in peripheral blood (n = 8) using the micronucleus assay in the same cohorts of mice.