Human cancer tissue Raw sequence reads
This study aims to comprehensively characterize the intratumoral microbiome in a large cohort of 226 Chinese patients with colorectal cancer (CRC) using 16S rRNA gene amplicon sequencing. The primary goals are to delineate the microbial composition, diversity, and community structure within the tumor microenvironment, and to investigate the associations between specific intratumoral bacterial taxa and critical clinicopathological features, including tumor stage, histological grade, lymph node metastasis, and perineural invasion status. The relevance of this research is substantial: it addresses the growing need to understand how intratumoral microbes contribute to the heterogeneity and progression of CRC. By identifying potential microbial biomarkers, this study may offer novel insights for early diagnosis, prognostic prediction, and the development of microbiome-targeted therapeutic strategies. Furthermore, the large-scale dataset generated from this project will serve as a valuable public resource for the broader research community to explore host-microbe interactions in colorectal carcinogenesis.