Single-cell transcriptome sequencing of bone marrow cells in renal osteodystrophy
We performed single-cell RNA sequencing (scRNA-seq) on bone marrow cells isolated from the femurs and tibiae of mice. Two groups were analyzed: a renal osteodystrophy (ROD) group and a healthy control group. For each group, three biological replicates were generated, each pooling three femurs and three tibiae from three animals. Cells were dissociated using collagenase I and dispase II digestion, followed by 40-micron filtration and erythrocyte lysis. Cell viability was assessed by trypan blue exclusion. This dataset aims to characterize the cellular heterogeneity and transcriptional alterations in the bone marrow microenvironment associated with renal osteodystrophy at single-cell resolution by comparing diseased and healthy samples.