Nasal microbiome dynamics during MRSA decolonization
16S rRNA (v3v4) amplicon sequences from a prospective study of the nasal microbiome in asymptomatic MRSA-carriers (N=34) undergoing decolonization treatment. Samples were collected from the anterior nares at 13 selected timepoints including the day before treatment initiation (day 0), daily during the first week after treatment initiation (days 1 through 7), weekly from day 7 to day 30, and at two late follow-up time points after three and six months, respectively. In addition, throat swabs were collected at day 0, day 7, and day 30. Participants were recruited during 2018 at the MRSA Knowledge Center, which is part of the Department of Clinical Microbiology at Hvidovre Hospital servicing part of the Capital Region of Denmark. Thirteen participants (38%) tested MSRA-positive in the nares, throat and/or perineum by culture during the routine follow-up testing in the healthcare system performed 30 days after completion of treatment, whereas 21 (62%) were successfully decolonized. Amplicon libraries were sequenced on an Illumina MiSeq using a 600-cycle reagent kit.
16S rRNA (v3v4) amplicon sequences from a prospective study of the nasal microbiome in asymptomatic MRSA-carriers (N=34) undergoing decolonization treatment. Samples were collected from the anterior nares at 13 selected timepoints including the day before treatment initiation (day 0), daily during the first week after treatment initiation (days 1 through 7), weekly from day 7 to day 30, and at two late follow-up time points after three and six months, respectively. In addition, throat swabs were collected at day 0, day 7, and day 30. Participants were recruited during 2018 at the MRSA Knowledge Center, which is part of the Department of Clinical Microbiology at Hvidovre Hospital servicing part of the Capital Region of Denmark. Thirteen participants (38%) tested MSRA-positive in the nares, throat and/or perineum by culture during the routine follow-up testing in the healthcare system performed 30 days after completion of treatment, whereas 21 (62%) were successfully decolonized. Amplicon libraries were sequenced on an Illumina MiSeq using a 600-cycle reagent kit.