Tet-off-regulated Hac1 expression in S. cerevisiae
The aim of this study is to find target genes of Hac1, which is the transcription factor that is involved in transcriptional induction on the unfolded protein response (UPR) in yeasts including Saccharomyces cerevisiae. The Hac1 gene was cloned into the S. cerevisiae Tet-off-promoter plasmid pCM190, and the resulting plasmid was named as pCM190-Hac1. Then S. cerevisiae cells were transformed with the plasmid pCM190-Hac1. As the control, S. cerevisiae cells were transformed with the empty plasmid pCM190. Then, we performed mRNA-seq analysis to check the gene expression profiles of these transformants cultured in the absence of doxycycline to activate the Tet-off promoter.