Transcriptomic profiling of inflamed chondrocytes and synoviocytes in human
Inflammatory cytokines such as interleukin-1b (IL-1b) and tumor necrosis factor-a (TNF-a) play central roles in the pathogenesis of osteoarthritis and other joint diseases. To investigate the molecular responses of human joint cells, we established 3D in vitro cultures of primary articular chondrocytes and synoviocytes and stimulated them with IL-1b and TNF-a. RNA sequencing was performed to profile transcriptomic changes associated with cytokine exposure. This dataset provides a resource for exploring inflammatory signaling in human chondrocytes and synoviocytes and may contribute to the identification of therapeutic targets for joint disease.