16S rRNA Gene Amplicon Sequencing of Rice Rhizosphere and Non-rhizosphere Soil Bacterial Communities under Nitrogen Fertilization
This project contains 16S rRNA gene amplicon sequencing data from bacterial communities in rice rhizosphere and non-rhizosphere soils under three nitrogen fertilization levels. A controlled pot experiment was conducted with no nitrogen addition (N0), medium nitrogen input (N1; 100 mg N kg-1 soil), and high nitrogen input (N2; 300 mg N kg-1 soil), with six biological replicates per treatment. The V4 region of the bacterial 16S rRNA gene was amplified using primers 515F and 806R and sequenced using paired-end sequencing on an Illumina MiSeq platform. These data were generated to characterize bacterial community responses and putative microbial taxa associated with denitrification, anaerobic ammonium oxidation, and Feammox in rice rhizosphere and non-rhizosphere soils.