RNA-seq from macrophages stimulated with non-viable lactic acid bacteria
Objective: To explore the Effect of heat-inactivated Enterococcus faecalis EF-2001 (EF-2001) on gene expression change in macrophage.  RAW264 cells were stimulated with EF-2001 for 6 h. Total RNA was extracted from the cells before and after stimulation. After quality control (TapeStation) and library preparation (SMART-Seq mRNA), 150-base pair-end sequencing analysis was performed using NovaSeq system.