RNA-seq of BMDMs co-cultured with Epimedium-derived extracellular vesicles (Epimedium-EVs) versus untreated control for renal osteodystrophy study
BMDMs were co-cultured with Epimedium-derived EVs or left untreated (control) for 72 hours. Total RNA was extracted using Trizol reagent (Thermo Fisher, 15596018). RNA concentration and purity were quantified by NanoDrop ND-1000. RNA integrity was assessed using Bioanalyzer 2100 (Agilent Technologies) with all samples meeting RIN >7.0, yield >1 ug, and concentration >50 ng/uL. Polyadenylated mRNA was enriched using Oligo(dT) magnetic beads through two rounds of selection. mRNA was fragmented, and first-strand cDNA synthesis was performed using Invitrogen SuperScript II Reverse Transcriptase (cat. 1896649). After second-strand synthesis, cDNA fragments were end-repaired, adenylated, and ligated to Illumina sequencing adapters. The library was PCR-amplified, purified, and quality-checked. RNA sequencing was performed on both treated and untreated groups. This dataset is part of a study on renal osteodystrophy.