Genome-wide profiling of H4K12la transcriptional targets in microglia of WT and MGKO mice
We generated microglia-specific VCP knockout (MGKO) mice and confirmed that VCP loss in microglia disrupts hippocampal synaptic integrity and causes cognitive deficits. H4K12la was upregulated in MGKO microglia. We then performed H4K12la CUT&amp;Tag sequencing on wild-type and MGKO microglia: associated DNA fragments were amplified, labeled and sequenced via Illumina NovaSeq-PE150.