Sanger sequencing and ICE analysis of CRISPR-Cas9-edited embryos from four treatment groups.
This study evaluated CRISPR-Cas9 editing of the TYR gene in zona-intact, zona-free, hemi-embryo, and quarter-embryo treatment groups. Target regions were analysed by Sanger sequencing and ICE analysis to identify indels and characterize editing outcomes.
This study evaluated CRISPR-Cas9 editing of the TYR gene in zona-intact, zona-free, hemi-embryo, and quarter-embryo treatment groups. Target regions were analysed by Sanger sequencing and ICE analysis to identify indels and characterize editing outcomes.