Genomic evolution of Bacillus subtilis 168CA and its L-form bacteria LR2 under glucose or glucose-oleic acid conditions over 1500 generations
This BioProject investigates the genomic evolution of Bacillus subtilis 168CA and its L-form LR2. The strains were evolved for 1500 generations under optimal glucose or glucose with different oleic acid gradients, each with four lineages. For each strain and condition, four independent lineages were established and evolved. Specifically, for the wild-type Bacillus subtilis 168CA evolved in the glucose-only environment, the four lineages are named m1, m2, m3, and m4; while in the glucose-oleic acid environment, the lineages are denoted as n1, n2, n3, and n4. Similarly, for the L-form bacteria LR2, the four lineages evolved in the glucose environment are p1, p2, p3, and p4, and those evolved in the glucose-oleic acid environment are q1, q2, q3, and q4. Sequencing data will reveal genetic adaptations and advance microbial evolution research.