Transcriptome sequencing of foot tissue from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus
This project describes the transcriptome sequencing of foot tissue from Bathymodiolus azoricus, a mytilid mussel endemic to deep-sea hydrothermal vent fields along the Mid-Atlantic Ridge. RNA was extracted from a single individual (sample N1188) collected at the Menez Gwen hydrothermal vent field during the French-led 2013 Biobaz oceanographic campaign aboard the RV Pourquoi Pas?. Sequencing was performed by StabVida on the Illumina HiSeq 4000 platform (150 bp paired-end reads), using a KAPA Stranded Total RNA library preparation with Ribo-Zero rRNA depletion. This work was carried out between 2017 and 2019 while the submitter was contracted by the 3B's Research Group on Biomaterials, Biodegradables and Biomimetics, University of Minho, under the supervision of Dr Tiago Silva, head of the marine-based biomaterials team at the Institute 3B's. Foot tissue was targeted specifically for its potential to yield novel adhesive proteins, given the byssus-forming and substrate-attachment role of this tissue in mytilid mussels.