Transcriptome sequencing of Prunus mume for low-temperature stress
This study aims to uncover the molecular regulatory network underlying cold stress response in Prunus mume, an important woody ornamental flower with weak cold resistance. We performed RNA-seq transcriptome sequencing on samples treated with low temperature at four time points (0 h control, 12 h, 24 h, 48 h), with three biological replicates for each group. Total RNA isolated from plant tissues was used to construct paired-end sequencing libraries on the Illumina platform. The raw sequencing reads will be utilized to quantify gene expression levels, screen cold-induced differentially expressed genes, and conduct GO/KEGG functional enrichment to identify core genes and critical metabolic pathways that mediate cold tolerance. As a valuable ornamental germplasm, the transcriptomic raw data of cold-treated Prunus mume provided here offers fundamental public resources for dissecting cold adaptation mechanisms of woody flowering plants and facilitates molecular breeding of cold-resistant mei cultivars in future horticultural research.