An rRNA-depleted whole-transcriptome dataset
This dataset presents high-resolution epitranscriptomic profiles (focusing on tRNA, snoRNA, lncRNA, and circRNA) of a stable wheat-barley 7H chromosome addition line and its parent cultivars, winter wheat Mv9 and winter barley Igri. An rRNA-depleted RNA-seq approach was utilized to capture the expression dynamics and epitranscriptomic landscapes of these non-coding and regulatory RNA species across the target genomes. The plant materials were cultivated under controlled, cold-acclimated conditions prior to leaf tissue collection. Sequencing libraries were analyzed in paired-end mode to ensure robust coverage of gene-rich and regulatory regions.The resulting dataset accurately reflects the species-specific transcriptomic and epitranscriptomic signatures of the parents alongside the combined profiles of the addition line. This resource allows the plant genomics community to investigate the epitranscriptomic stability of alien chromosome additions. Furthermore, it provides data for exploring how post-transcriptional RNA modifications and non-coding RNA networks behave within hybrid nuclei and the potential epitranscriptomic mechanisms underpinning phenotype formation in wide crosses.