RNA-seq of TNRC6A, TNRC6B and TNRC6C knockout HeLa cells with paralog re-expression
The human GW182 family comprises three paralogs, TNRC6A, TNRC6B and TNRC6C, which act downstream of Argonaute proteins in microRNA-mediated gene silencing. To dissect the contribution of each paralog to the cellular transcriptome, we generated HeLa cell lines in which TNRC6A, TNRC6B or TNRC6C was individually knocked out, and re-expressed the corresponding paralog in each knockout background. Poly(A)-selected mRNA sequencing (DNBSEQ-T7, paired-end 150 bp) was performed on eight samples: untransfected wild-type cells, wild-type cells transfected with an empty vector, each of the three knockout lines transfected with an empty vector, and each knockout line transfected with a vector expressing the corresponding TNRC6 paralog.