Pediococcus acidilactici strain:0110<TAT-1 Genome sequencing
Pediococcus acidilactici has been noted for its production of natural antimicrobial compounds that effectively inhibit the growth of various microorganisms, including significant pathogens like Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. This antimicrobial prowess is primarily attributed to pediocin, a heat-stable, wide-spectrum antimicrobial peptide. While these antimicrobial compounds are present in the cell-free supernatant of P. acidilactici, our previous study suggested that organic acids are the principal contributors. This discrepancy raises the question of whether the variation in pediocin production is due to strain-dependent factors or if there might be discrepancies in the metabolic pathways responsible for P. acidilactici's antimicrobial capabilities. To address these queries, the study proposes using Whole Genome Sequencing (WGS), an advanced technique in genetics and genomics. WGS enables the comprehensive decoding of the entire DNA sequence of an organism, encompassing all genes, non-coding sequences, and regulatory elements. The primary objective of this research is to identify the strain and specific genomic elements within our P. acidilactici isolate that govern the production of pediocin based on WGS results and bioinformatic analysis. By uncovering the genetic basis for pediocin production, the study aims to shed light on the factors responsible for variations in antimicrobial potency among different strains of P. acidilactici. Additionally, the study will assess the potential and safety of employing P. acidilactici in food and beverage applications by identifying its metabolic pathways, antibiotic resistance genes, and virulence factors.