The Mechanism of PEDV-Induced Exosomes from Intestinal Epithelial Cells Inhibiting Macrophage Migration and Phagocytic Function_lncRNA_mRNAseq
To investigate the impact of the long non-coding RNA TCONS_00002083 (abbreviated as LncRNA TS-2083), which exhibits the highest expression level in exosomes, on the transcriptional profile of macrophages, we constructed a lentivirus overexpressing LncRNA TS-2083 (100 MOI) and used it to infect 3D4/21 macrophages. A lentivirus carrying an empty vector was employed as a negative control. This approach allowed a comprehensive analysis of the effects of LncRNA TS-2083 overexpression on the transcriptional profile of macrophages.