Transcriptomic landscape of podocyte injury induced by doxorubicin
Organism: Mus musculusExperiment type: Expression profiling by high throughput sequencingSummary:Podocyte injury is a critical event driving the progression of glomerular diseases. Doxorubicin (DOX) is widely used to establish podocyte injury models, but the genome-wide transcriptional changes underlying this injury remain incompletely understood. Here, we performed RNA sequencing in immortalized mouse podocytes (MPC-5 cells) to characterize the transcriptomic landscape of DOX-induced podocyte injury. MPC-5 cells were treated with 0.1 ug/mL DOX for 24 hours to induce injury, with untreated cells as controls. Total RNA was extracted from three biological replicates per group and sequenced on the NovaSeq X Plus platform. This dataset provides a comprehensive resource for understanding the molecular mechanisms of podocyte injury and identifying potential therapeutic targets for glomerular diseases. The raw sequence data have been deposited in the NCBI Gene Expression Omnibus (GEO) and are accessible through the Series record.Overall design:Examination of transcriptomic changes in MPC-5 podocytes following DOX-induced injury. Three biological replicates per condition (control and DOX-treated).