Amplicon sequencing of Marburg viral sequences to characterize bat APOBEC3-mediated RNA editing
This project investigates RNA editing of Marburg viral sequences mediated by bat APOBEC3 proteins. Total RNA was extracted from HEK293T cells co-transfected with Marburg virus fragments and bat APOBEC3 expression plasmids. RNA was reverse-transcribed into cDNA, and target regions were amplified by PCR, gel-purified, and subjected to amplicon sequencing to identify and quantify APOBEC3-mediated C-to-U RNA editing events.