Longitudinal profiling of gut microbiota associated with intravenous glucose load in NICU neonates.
We aimed to investigate the relationship between intravenous glucose load, specifically the glucose infusion rate (GIR), and early gut microbiota development in neonates. A total of 90 stool samples from 10 neonates admitted to a neonatal intensive care unit (NICU) were analyzed. Stool samples were collected daily from birth through postnatal day 14. Microbiota profiles of each sample were analyzed by meta-16S analysis targeting the V3-V4 region of the bacterial 16S rRNA gene. We also attempted to evaluate the functional maturation of the microbiota and identify predictive microbial taxa that distinguish clinical phenotypes using machine learning models.