4. The antiproliferative effects and potential mechanisms of Mahuang Fuzi Xixin Decoction on lung adenocarcinoma cells: An integrated network pharmacology, multi-cohort transcriptomics and in vitro experimental validation
CCK-8 and colony formation assays were performed in A549 and NCI-H1299 cells. Network pharmacology, seven GEO cohorts, machine learning, TCGA-LUAD, paired transcriptomes, survival, immune-infiltration, and single-cell analyses were integrated to prioritize genes. Molecular docking, 100-ns molecular dynamics simulations, and MM/GBSA calculations assessed compounds targeting ADRB2.