Cadmium-induced transcriptome dynamics in the seagrass Zostera marina
Experimental Design &amp; Treatment SetupUniform Z. marina seedlings (5 individuals per 3 L glass bottle) were incubated in sterilized seawater under controlled conditions (20 C, 18 days). Three groups were established:1. MC (Control): No additional treatment2. MK (Cd stress): Exposed to 500 uM cadmium chloride3. MM (Cd + Microbe): Exposed to 500 uM cadmium chloride and inoculated with E. aurantiacum S11 (final density ~10^4 CFU/mL)All systems were sealed to prevent evaporation and maintained under the same conditions used during pre-experiment seedling acclimation.