Diet analysis of the Crested Serpent-eagle on Ishigaki Island in Ryukyu Archipelago
We analysed the diet of the Crested Serpent-eagle on Ishigaki Island which frequently prey on introduced cane toads. We performed DNA metabacording for their fecal samples to identify its prey DNA. Four primer sets were used for the anlysis: Ecoprimer for vertebrate mitochondrial 12S rRNA, MtAnr for anuran mitochondrial 16S rRNA, ewD/E for earthworm mitochondrial 16S rRNA, and MiDeca for invertebrate mitochondrial 16S rRNA.