Epitranscriptomic and transcriptomic profiling of colonic mucosa before and after washed microbiota transplantation in patients with irritable bowel syndrome
This project investigates the transcriptomic and m6A epitranscriptomic alterations in colonic mucosal tissues from patients with irritable bowel syndrome (IBS) before and after washed microbiota transplantation (WMT). Paired RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation sequencing (MeRIP-seq) were performed to characterize changes in gene expression and m6A RNA methylation associated with WMT treatment. These data provide a resource for studying host epitranscriptomic remodeling following microbiota-based therapy.