GEMiNI-seq for wildtype and variant Cas nuclease accession into the nucleosome
The GEMiNI-seq assay evaluates how chromatin structure influences gene editor activity by using a library of nucleosome-positioning sequences to assess editing across all potential target sites within the nucleosome. By quantifying cleavage at each site, GEMiNI-seq generates a comprehensive map of gene editor accessibility, identifying the most permissive targets. GEMiNI-seq has been applied to test the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas nucleases, wtSpCas9, wtLbCas12a, wtAsCas12a, evoSpCas9, HIFIv1, LbCas12a ULTRA, and AsCas12a ULTRA.