Gut microbiome alterations associated with chronic restraint stress and sucralose exposure in mice
This project investigates the effects of chronic restraint stress and subsequent sucralose exposure on the gut microbiome in mice. Chronic restraint stress was used to establish a stress induced model relevant to disorders of gut brain interaction. Following stress exposure, mice received drinking water containing either vehicle, 0.03 percent sucralose, or 0.1 percent sucralose. Cecal contents were collected and analyzed using full length 16S rRNA gene sequencing on the PacBio platform. The study aims to characterize stress and sucralose associated alterations in microbial community composition and diversity and to identify microbial taxa associated with changes in intestinal physiology and host metabolism. These data provide a resource for understanding the impact of chronic stress and non nutritive sweetener exposure on host microbiome interactions.