Differential gene expression of earthworms in response to MnCl and engineered nanomaterial exposure (MCNM, 4n-4, 4n-6) across acute and time-series designs
Experiment 1 is divided into 2 sets. In the first set 2x4 samples were obtained from earthworms (Eisenia fetida) exposed to control and multi-component 4n-6 nanomaterial treatments, respectively. In the second set 2x4 samples were obtained from worms exposed to negative and positive control (Manganese chloride) treatments, respectively. Experiment 2 is obtained from an experiment in which earthworms (Eisenia fetida) exposed to control and 4n-4 nanomaterial treated soil, respectively. Worms were exposed to either of the treatments for 2, 3, 4 or 7 days, after which they were harvested. 4 jars were used per treatment and exposure time, which resulted in a total of 32 jars. Every jar contained 4 worms of which one was selected for RNA-seq. Furthermore, 4 worms were frozen at the start of the experiment, which served as the references. Experiment 3 is obtained from an experiment in which earthworms (Eisenia fetida) exposed to control and multi-component 4n-6 nanomaterial treated soil, respectively. Worms were exposed to either of the treatments for 2, 3, 4 or 7 days, after which they were harvested. 4 jars were used per treatment and exposure time, which resulted in a total of 32 jars. Every jar contained 4 worms of which one was selected for RNA-seq. Furthermore, 4 worms were frozen at the start of the experiment, which served as the references.
Experiment 1 is divided into 2 sets. In the first set 2x4 samples were obtained from earthworms (Eisenia fetida) exposed to control and multi-component 4n-6 nanomaterial treatments, respectively. In the second set 2x4 samples were obtained from worms exposed to negative and positive control (Manganese chloride) treatments, respectively. Experiment 2 is obtained from an experiment in which earthworms (Eisenia fetida) exposed to control and 4n-4 nanomaterial treated soil, respectively. Worms were exposed to either of the treatments for 2, 3, 4 or 7 days, after which they were harvested. 4 jars were used per treatment and exposure time, which resulted in a total of 32 jars. Every jar contained 4 worms of which one was selected for RNA-seq. Furthermore, 4 worms were frozen at the start of the experiment, which served as the references. Experiment 3 is obtained from an experiment in which earthworms (Eisenia fetida) exposed to control and multi-component 4n-6 nanomaterial treated soil, respectively. Worms were exposed to either of the treatments for 2, 3, 4 or 7 days, after which they were harvested. 4 jars were used per treatment and exposure time, which resulted in a total of 32 jars. Every jar contained 4 worms of which one was selected for RNA-seq. Furthermore, 4 worms were frozen at the start of the experiment, which served as the references.