Transcriptomic analysis of hematopoietic stem/progenitor cells and CD8-positive T cells in Mir14255A>G knock-in mice
This project investigates transcriptional changes associated with the Mir14255A>G mutation in hematopoietic stem/progenitor cells and CD8-positive T cells. Bone marrow cells from wild-type or Mir14255A>G heterozygous knock-in mice were transplanted into irradiated recipient mice. Hematopoietic stem/progenitor cells and CD8-positive T cells were collected from wild-type controls, pre-leukemic mutant mice, and mutant mice with T-cell leukemia. RNA sequencing was performed to characterize gene expression changes during leukemia development and to examine the molecular effects of the Mir14255A>G mutation.