Gut microbiota profiling in acute sleep-deprived mice following diosgenin pretreatment
This study investigated alterations in the gut microbiota induced by 24-hour acute sleep deprivation and evaluated the modulatory effects of prophylactic diosgenin treatment. Fecal samples were collected from control mice, mice subjected to 24-hour sleep deprivation, and mice pretreated with diosgenin at 90 mg/kg/day by oral gavage for three weeks before sleep deprivation, with six biological replicates per group. Full-length bacterial 16S rRNA genes were amplified using the 27F and 1492R primers and sequenced on the PacBio Sequel IIe platform. The resulting dataset was used to characterize microbial diversity, community structure, taxonomic composition, differential bacterial taxa, and predicted microbial functional potential among the three experimental groups.