CsA triggers paraptosis in senescent cells
Proliferating IMR-90 cells, doxorubicin-induced senescent IMR-90 cells, and replicative senescent IMR-90 cells were treated with either DMSO or 5 ÂµM CsA for 3 days. Each condition included three biological replicates. Quality control of raw sequencing reads was initially performed using FastQC (version 0.12.1) to assess data quality metrics. Following quality assessment, raw reads were subjected to adapter clipping and the removal of low-quality reads using Trim Galore (version 0.6.10) with default parameters. After preprocessing, reads were aligned to the GRCh38.p13 reference human genome from the NCBI RefSeq database using STAR software (version 2.7.11a). Gene-level quantification was performed using RSEM to obtain expected_counts and TPM values.For differential expression analysis, the expected_counts from RSEM were imported into DESeq2 (Bioconductor) via the tximport package.