Drosophila whole genome sequencing dataset from our EMS-based genetic screen to identify genes required for axonal maintenance.
Drosophila EMS-based genetic screen of the X-chromosome to screen for genes required for axonal maintenance. Flies were mutagenized using 10mM EMS. Before genome isolation, mutant flies were either crossed to the FRT19A background or inter se, to obtain FRT19A*/FRT19A or FRT19A*/FRT19A*. 50 virgins were collected and starved before genome isolation. Libraries were prepared using the Illumina Nextera Flex library prep kit, and sequenced on a NextSeq 500.
Drosophila EMS-based genetic screen of the X-chromosome to screen for genes required for axonal maintenance. Flies were mutagenized using 10mM EMS. Before genome isolation, mutant flies were either crossed to the FRT19A background or inter se, to obtain FRT19A*/FRT19A or FRT19A*/FRT19A*. 50 virgins were collected and starved before genome isolation. Libraries were prepared using the Illumina Nextera Flex library prep kit, and sequenced on a NextSeq 500.