Single-cell Transcriptomic and Immune Repertoire Studies of IBD in Prospective Registry in IBD Study at MGH
In inflammatory bowel disease (IBD) patients, a breakdown in the regulatory mechanisms enforcing tolerance to the intestinal microbiota leads to chronic inflammation. CD4 T cells mediate both tolerizing and pathological immune responses to members of the microbiota. To identify disease-associated CD4 T cell phenotypes and TCR clonal dynamics we profiled T cells from intestinal tissues of IBD patients with Crohn's Disease or ulcerative colitis, along with healthy tissue from diverticulitis patients, using single-cell transcriptomics (scRNA-seq) and immune repertoire profiling of TCR sequences (scV(D)J-seq). Parallel profiling of SusC-HLA class II tetramer sorted T cells allowed us to link functional profiles with microbiome-specificity. Intestinal samples were processed by stripping... (for more see dbGaP study page.)