Genetic dissection of a powdery mildew resistance locus on chromosome arm 6AL of Triticum boeoticum accession PI 427948
Triticum boeoticum, as one of the wild diploid wheat species, harbors numerous disease-resistance genes in its genome that are beneficial for wheat improvement. In this study, an F2 population was constructed by crossing the powdery mildew-resistant T. boeoticum accession PI 427948 with the susceptible accession PI 427560. Genetic analysis revealed that the resistance in PI 427948 is controlled by a single dominant gene, PmPI427948. Bulked segregant exome capture sequencing (BSE-Seq) conducted on the F2 population revealed a significant peak on the long arm of chromosome 6A (6AL). Subsequently, through linkage analysis, PmPI427948 was delimited to a genetic interval of 0.138 cM on 6AL, flanked by markers XTb6AL04 and XTb6AL05, both with genetic distances of 0.069 cM. Based on the reference genome of T. boeoticum accession TA299, PmPI427854 corresponds to an approximate 690-kb physical region of 635.96-636.65 Mb.