Bulk RNA sequencing of COL4A1 mutant and isogenic control iPSC-derived astrocytes
This study investigates transcriptomic differences between induced pluripotent stem cell (iPSC)-derived astrocytes carrying a COL4A1 mutation and an isogenic control line using bulk RNA sequencing.  iPSC lines were differentiated into astrocytes under standardized culture conditions. Total RNA was extracted from three independent biological replicates per condition. Sequencing libraries were prepared using standard Illumina-compatible RNA-seq library preparation protocols, with poly(A) selection or equivalent enrichment methods.  Paired-end sequencing was performed on an Illumina NovaSeq platform to generate raw FASTQ files. The dataset includes six biological samples in total, comprising three mutant and three isogenic control replicates.  This dataset provides a resource for comparative transcriptomic analysis of COL4A1-associated cellular phenotypes in human iPSC-derived astrocyte models.
This study investigates transcriptomic differences between induced pluripotent stem cell (iPSC)-derived astrocytes carrying a COL4A1 mutation and an isogenic control line using bulk RNA sequencing.  iPSC lines were differentiated into astrocytes under standardized culture conditions. Total RNA was extracted from three independent biological replicates per condition. Sequencing libraries were prepared using standard Illumina-compatible RNA-seq library preparation protocols, with poly(A) selection or equivalent enrichment methods.  Paired-end sequencing was performed on an Illumina NovaSeq platform to generate raw FASTQ files. The dataset includes six biological samples in total, comprising three mutant and three isogenic control replicates.  This dataset provides a resource for comparative transcriptomic analysis of COL4A1-associated cellular phenotypes in human iPSC-derived astrocyte models.