Key Drivers and Underlying Biological Mechanisms of Anaerobic Oxidation of Methane (AOM) in Constructed Wetlands
This project investigates the microbial mechanisms underlying anaerobic oxidation of methane (AOM) in constructed wetlands (CWs). Soil samples from CWs were analyzed using metagenomic sequencing together with 13CH4 isotope tracing, physicochemical measurements, and electrochemical characterization. The metagenomic datasets were generated to characterize microbial community composition, identify key methane-cycling microorganisms, and reconstruct metabolic pathways potentially involved in AOM and extracellular electron transfer. Particular attention was given to anaerobic methanotrophic archaea, methanogenic archaea, and functional genes associated with methane metabolism and electron transport. Integration of microbial, environmental, and electrochemical data was used to explore the relationships between AOM activity, environmental factors, and microbial metabolic potential. These datasets provide a resource for understanding the microbial ecology and potential electron-transfer mechanisms of AOM in constructed wetlands and may contribute to improved assessment of methane mitigation in wetland systems.