Transcriptome analysis of anther under high temperature and normal temperature conditions
High temperature is a major environmental stress that severely disrupts pollen development and male fertility in cotton. To uncover the molecular mechanisms governing heat stress responses during anther development, we performed RNA sequencing (RNA-Seq) using anther samples harvested from cotton plants under high-temperature and normal-temperature (control) conditions. Differential gene expression analysis was performed to identify heat-responsive genes and pathways associated with pollen development. This study aims to dissect the transcriptional regulatory network underlying anther thermotolerance in cotton and provide insights into breeding heat-tolerant cotton varieties.