RNA-seq of common toad tadpoles (Bufo bufo)
We sampled common toad tadpoles of the developmental stage 31 according to Gosner 1960. This stage marks the begin of hindlimb development which is paddle-shaped.We sampled the cornified "teeth/sheaths" with adjacent skin of the tadpoles and submitted them to RNA-bulk-seq. Regular skin from the back of the tadpole was sampled and sequenced as control sample for the differential expression analysis.The tadpole sampling happened according to the local guidelines of the local government of the region of Niederoesterreich (Lower Austria). The sampling permission for common toad tadpoles was granted to Attila Placido Sachslehner (RU5-BE-2095/001-2024).