Whole-genome and transcriptome sequencing of NT2.5, NT2.5LM, and NT2.5LV, a HER2-positive murine breast cancer cell line series with divergent organ-preferential metastasis
This BioProject contains whole-genome sequencing (WGS) and bulk RNA-sequencing data for an isogenic series of HER2-positive murine breast cancer cell lines: the parental NT2.5 line and two organ-preferential metastatic derivatives, NT2.5LM (lung-enriched) and NT2.5LV (liver-enriched). The derivatives were generated from NT2.5 by iterative in vivo selection in immunocompetent neu-N transgenic FVB/N mice. WGS was performed on three samples per cell line to characterize the somatic mutational landscape, copy-number architecture, and clonal evolution across the parental and metastatic lines. Bulk RNA-sequencing was performed on the same cell line series to define the transcriptional programs associated with lung- vs. liver-preferential metastatic colonization. Together, these data support an integrated genomic and transcriptomic characterization of how organ-preferential metastasis is shaped in HER2-positive breast cancer on a conserved genomic background.