Time-course transcriptome profiling of bikinin-treated alfalfa leaf disks
This BioProject contains time-course bulk RNA-sequencing data from leaf disks of Medicago sativa L. cv. Zhongmu No. 1. The experimental design included untreated 0-h baseline samples and mock-control and bikinin-treated samples collected at 12, 24, and 48 h. Three independent biological replicates were prepared for each group, resulting in seven experimental groups and 21 paired-end RNA-seq libraries. The dataset was generated to characterize temporal transcriptional responses associated with bikinin treatment, xylem differentiation, secondary cell-wall formation, and lignification-related processes in alfalfa leaf disks.