Transcriptomic profiling of human hepatic stellate cells (LX-2) in liver fibrosis model and therapeutic intervention
Hepatic stellate cell (HSC) activation is a central pathogenic event in liver fibrosis. In this study, transcriptomic profiling (RNA-Seq) was conducted on human hepatic stellate cells (LX-2) to investigate the molecular mechanisms of HSC activation and therapeutic regression. The study includes three experimental groups: untreated control cells (Control, n=3), fibrotic model cells (Model, n=3), and therapeutic intervention-treated cells (Treatment, n=3). High-throughput paired-end RNA sequencing was performed to identify differentially expressed genes (DEGs) and key signaling pathways involved in extracellular matrix deposition, oxidative stress, and cell activation.