Genome-wide KAKU4-eYFP ChIP-seq of Arabidopsis thaliana kaku4 complemented and untagged control plants at seedling (10 days) and 120-day rosette stages
Chromatin immunoprecipitation sequencing (ChIP-seq) was used to map the genome-wide binding profile of the inner nuclear envelope protein KAKU4 in Arabidopsis thaliana. A functional ProKAKU4:KAKU4-eYFP transgene (Goto et al., Plant Cell 2014), which complements the kaku4-3 mutant, and an untagged kaku4 (SALK_076754) control line were analysed. Young samples consisted of 10-day-old seedlings (n = 3 biological replicates per genotype, pooling ~12 seedlings per replicate). Old samples consisted of 120-day short-day rosettes, where senescence leaves from 3–4 plants were pooled per replicate (n = 3 replicates). Together enabling comparison of KAKU4 occupancy between juvenile and long-term aged tissues.
Chromatin immunoprecipitation sequencing (ChIP-seq) was used to map the genome-wide binding profile of the inner nuclear envelope protein KAKU4 in Arabidopsis thaliana. A functional ProKAKU4:KAKU4-eYFP transgene (Goto et al., Plant Cell 2014), which complements the kaku4-3 mutant, and an untagged kaku4 (SALK_076754) control line were analysed. Young samples consisted of 10-day-old seedlings (n = 3 biological replicates per genotype, pooling ~12 seedlings per replicate). Old samples consisted of 120-day short-day rosettes, where senescence leaves from 3–4 plants were pooled per replicate (n = 3 replicates). Together enabling comparison of KAKU4 occupancy between juvenile and long-term aged tissues.