Single cell RNAseq of uterine myeloid and CD45- cells in the steady state
The aim of this experiment was to identify specific subsets of monocytes, dendritic cells, macrophages and structural cells including epithelial cells, endothelial cells and stromal cells in the murine uterus, across all reproductive cycle stages via single cell RNA sequencing. Samples were FACS-enriched separately for populations of CD45+Ly6G-SiglecF-CD3-CD5-CD19-CD20- cells expressing MHC class II and/or CD11b (myeloid) and CD45- structural cells.
The aim of this experiment was to identify specific subsets of monocytes, dendritic cells, macrophages and structural cells including epithelial cells, endothelial cells and stromal cells in the murine uterus, across all reproductive cycle stages via single cell RNA sequencing. Samples were FACS-enriched separately for populations of CD45+Ly6G-SiglecF-CD3-CD5-CD19-CD20- cells expressing MHC class II and/or CD11b (myeloid) and CD45- structural cells.