A ground surface rolling method for detecting environmental DNA of terrestrial animals
Demonstrated the usefulness of the method of collecting environmental DNA by rolling on the ground surface for monitoring terrestrial animals in natural environments. Specifically, ground surface samples were collected every two weeks for 14 months in a secondary natural environment, the Forest of Toyota in Japan. Metabarcoding analysis was used to detect bird and mammal DNA in the samples and the results were compared with animal survey results by other methods.