ChIP-seq of C4-2 cells treated with DMSO or tigecycline
Following treatment with tigecycline or DMSO for 24 h, cells were cross-linked with formaldehyde. Chromatin was sheared to an average size of approximately 200 bp using a non-contact ultrasonic disruptor. The fragmented chromatin was incubated overnight with anti-FOXA1 antibody coupled to magnetic beads. Immunoprecipitated complexes were then eluted, reverse cross-linked, and purified. Immunoprecipitated DNA was used to construct the sequencing library. Paired-end sequencing was carried out on an Illumina NextSeq 500 instrument.