Plasma Cell-Free DNA Sequencing from Cancer-Free and Non-HPV-Associated Cancer Controls
This project provides plasma cell-free DNA sequencing data from cancer-free individuals and patients with non-HPV-associated cancers used as controls for the development and validation of a whole-genome HPV liquid biopsy assay. The control cohort supports assessment of assay specificity, background sequencing signals, and the ability to distinguish HPV-associated malignancies from samples without HPV-associated disease. These data complement sequencing from patients with HPV-associated cancers across multiple anatomic sites and contribute to the evaluation of HPV-DeepSeek, a targeted whole-genome HPV sequencing approach for detecting circulating tumor HPV DNA in plasma. The control dataset is relevant to establishing analytical and diagnostic performance, identifying potential sources of nonspecific signal, and supporting the development of accurate liquid biopsy methods for pan-HPV-associated cancer detection.