Effect of Temperature on Gene Expression of Escherichia marmotae
Temperature is one of the first environmental cues encountered by bacteria upon entering a mammalian host. This study investigated the effects of temperature on the transcriptome and proteome of Escherichia marmotae and Escherichia coli. Previous studies demonstrated that temperature affects motility in Escherichia marmotae. We compared global gene expression and protein abundance in strains grown under static conditions at 28 C and 37 C. RNA sequencing and quantitative proteomics were used to characterize temperature-dependent changes and determine whether these responses are conserved between Escherichia marmotae and Escherichia coli.