Whole-genome sequencing of adaptive laboratory evolution of Escherichia coli carrying an orthologous replacement of the essential metK gene
This BioProject contains whole-genome sequencing data from Escherichia coli K-12 MG1655 strains subjected to adaptive laboratory evolution following replacement of the native metK gene with an ortholog from Ureaplasma urealyticum. Sequenced isolates include evolved clones together with the corresponding ancestral and engineered strains. The data were generated to identify mutations associated with adaptation to an inefficient methionine S-adenosyltransferase and to investigate the genetic basis of metabolic adaptation.