Time course of Embryonic Genome Activation Following Kdm5b Overexpression using SMART-seq2
RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 µg/µL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as “Noninj”. The embryos were cultured until collection at 20 h, 23 h, and 26 h post-IVF.  A note on replicates: The replicate annotation refers to the number of times the specific condition was collected, i.e., the N number. It does not reflect the experimental batch.  For the IVF batch, please refer to the sample sheet provided in the accompanying Git repository.
RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 µg/µL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as “Noninj”. The embryos were cultured until collection at 20 h, 23 h, and 26 h post-IVF.  A note on replicates: The replicate annotation refers to the number of times the specific condition was collected, i.e., the N number. It does not reflect the experimental batch.  For the IVF batch, please refer to the sample sheet provided in the accompanying Git repository.
European Bioinformatics Institute