Aspergillus fumigatus transcriptome during growth in SDB, SCFM2, and co-culture with Pseudomonas aeruginosa PA14
This study examines transcriptional changes in Aspergillus fumigatus across different growth environments and in response to bacterial co-culture. A. fumigatus was grown in Sabouraud Dextrose Broth (SDB), synthetic cystic fibrosis medium 2 (SCFM2), and in co-culture with Pseudomonas aeruginosa in SCFM2. Experimental conditions include A. fumigatus monoculture in SDB, monoculture in SCFM2, and co-culture with P. aeruginosa in SCFM2, each performed in biological triplicate. Total RNA was extracted from cultures and rRNA-depleted prior to library preparation and sequencing. This dataset enables analysis of fungal transcriptional adaptation to cystic fibrosis-relevant media and identification of gene expression change associated with bacterial interaction.
This study examines transcriptional changes in Aspergillus fumigatus across different growth environments and in response to bacterial co-culture. A. fumigatus was grown in Sabouraud Dextrose Broth (SDB), synthetic cystic fibrosis medium 2 (SCFM2), and in co-culture with Pseudomonas aeruginosa in SCFM2. Experimental conditions include A. fumigatus monoculture in SDB, monoculture in SCFM2, and co-culture with P. aeruginosa in SCFM2, each performed in biological triplicate. Total RNA was extracted from cultures and rRNA-depleted prior to library preparation and sequencing. This dataset enables analysis of fungal transcriptional adaptation to cystic fibrosis-relevant media and identification of gene expression change associated with bacterial interaction.
European Bioinformatics Institute