micro-4C for investigating the interaction among HS2 core element and all other regions in ß-globin locus in K562 and HL-60 cells
GATA1-binding sites constituted essential functional elements within the HS2 enhancer, enabling transcription factor recruitment, enhancer activation, and long-range chromatin interactions at the ß-globin locus. In K562 cells, a human erythroleukemia line that expresses fetal and embryonic globin genes and possesses an active ß-globin LCR, GATA-1 occupancy at HS2 was demonstrated by previous study. We performed micro-4C to investigate the interactions among HS2 core element and all other regions in ß-globin locus in K562 and HL-60 myeloid leukemia cells (as a negative control). Erythroid-specific DNA interactions can be readily detected
GATA1-binding sites constituted essential functional elements within the HS2 enhancer, enabling transcription factor recruitment, enhancer activation, and long-range chromatin interactions at the ß-globin locus. In K562 cells, a human erythroleukemia line that expresses fetal and embryonic globin genes and possesses an active ß-globin LCR, GATA-1 occupancy at HS2 was demonstrated by previous study. We performed micro-4C to investigate the interactions among HS2 core element and all other regions in ß-globin locus in K562 and HL-60 myeloid leukemia cells (as a negative control). Erythroid-specific DNA interactions can be readily detected
European Bioinformatics Institute