Single-cell RNA sequencing analysis of human aortic smooth muscle cells under mechanical stretch and inflammatory stimulus
To investigate the heterogeneity of human aortic SMCs in culture, we performed scRNA-seq of SMCs under three conditions: static, 10% stretch (6 h), and TNF stimulation (10 ng/mL, 24 h) using the BD Rhapsody™ microwell-based cell capture platform. Approximately 28,000 cells were captured, and library preparation of ~14,000 cells was performed. Indexed libraries were pooled and sequenced on Illumina NovaSeq X Plus (2 × 150 bp, 10B flow cell, one lane), yielding ~2–2.5 billion paired-end reads, corresponding to an expected read-pair per cell of ~50,000.
To investigate the heterogeneity of human aortic SMCs in culture, we performed scRNA-seq of SMCs under three conditions: static, 10% stretch (6 h), and TNF stimulation (10 ng/mL, 24 h) using the BD Rhapsody™ microwell-based cell capture platform. Approximately 28,000 cells were captured, and library preparation of ~14,000 cells was performed. Indexed libraries were pooled and sequenced on Illumina NovaSeq X Plus (2 × 150 bp, 10B flow cell, one lane), yielding ~2–2.5 billion paired-end reads, corresponding to an expected read-pair per cell of ~50,000.
European Bioinformatics Institute