Transcriptomic profiling of Sox10-Venus-positive dorsal root ganglion cells after spared nerve injury in mice
This project investigates transcriptomic alterations in Sox10-Venus-positive cells of the dorsal root ganglia (DRGs) following peripheral nerve injury. A spared nerve injury (SNI) model was established in Sox10-Venus transgenic mice by ligation and transection of the tibial and common peroneal branches of the left sciatic nerve while leaving the sural nerve intact. Three weeks after SNI, lumbar DRGs were collected from the ipsilateral (SNI-operated) and contralateral (unoperated) sides. Venus-positive cells were isolated from the DRGs by fluorescence-activated cell sorting (FACS) and subjected to RNA sequencing. The dataset was generated to characterize injury-associated transcriptional changes in Sox10-Venus-positive, satellite glial cell-enriched populations and to investigate molecular pathways associated with satellite glial cell remodeling during chronic neuropathic pain.
BioProject