Genomic characterization of 101 Klebsiella pneumoniae isolates with carbapenem resistances
Non-duplicate Klebsiella pneumoniae isolates recovered from different clinical specimens at the “Agrippa Ionescu” Clinical Emergency Hospital during two study periods: July 2023–January 2024 and April 2024–May 2025. Species identification was performed using MALDI-ToF MS. Strains with decreased susceptibility to at least one carbapenem, were sent to the National Reference Center for Nosocomial and Antibiotic Resistant Infections at the Cantacuzino National Medico-Miliatry Institute for Research and Developement. Antimicrobial susceptibility testing followed EUCAST 2025 guidelines. WGS was carried out using Oxford Nanopore Technology (MinION and PromethION devices) to determine resistance genes, plasmid replicons and multilocus sequence types.
Non-duplicate Klebsiella pneumoniae isolates recovered from different clinical specimens at the “Agrippa Ionescu” Clinical Emergency Hospital during two study periods: July 2023–January 2024 and April 2024–May 2025. Species identification was performed using MALDI-ToF MS. Strains with decreased susceptibility to at least one carbapenem, were sent to the National Reference Center for Nosocomial and Antibiotic Resistant Infections at the Cantacuzino National Medico-Miliatry Institute for Research and Developement. Antimicrobial susceptibility testing followed EUCAST 2025 guidelines. WGS was carried out using Oxford Nanopore Technology (MinION and PromethION devices) to determine resistance genes, plasmid replicons and multilocus sequence types.
Cantacuzino National Military Medical Institute for Research and Development