Smart-Seq of female and male blastocysts from mothers bearing the OgtT931A mutation
40-bp PE mRNA-Seq of single E4 blastocysts generated through IVF of oocytes harvested from two F0 Ogt[T931A/+] females and from two littermate WT females, using the same WT sperm. Sex and presence of the mutation was established by PCR genotyping of the cDNA; mutation was confirmed by Sanger sequencing and sex was confirmed by in silico analysis of counts of Y-linked genes.
40-bp PE mRNA-Seq of single E4 blastocysts generated through IVF of oocytes harvested from two F0 Ogt[T931A/+] females and from two littermate WT females, using the same WT sperm. Sex and presence of the mutation was established by PCR genotyping of the cDNA; mutation was confirmed by Sanger sequencing and sex was confirmed by in silico analysis of counts of Y-linked genes.
European Bioinformatics Institute