Metabarcoding of sedimentary DNA from the Weddell Sea seafloor
V9 metabarcoding data, in mixed orientation. Data to publication:  target_gene = "18S", target_subfragment = "V9", nucleic_acid_extraction = "combined method from Armbrecht, L., Herrando-Pérez, S., Eisenhofer, R., Hallegraeff, G. M., Bolch, C. J. S., & Cooper, A. (2020). An optimized method for the extraction of ancient eukaryote DNA from marine sediments. Molecular Ecology Resources, 20(4), 906–919. https://doi.org/10.1111/1755-0998.13162", pcr_conditions = "initial denaturation:95degC_5min;34x denaturation:95degC_30s;annealing:57degC_45s;elongation:72degC_45s;final extension:72degC_5min",  pcr_primers = "1389F 5'-TTGTACACACCGCCC-3' and 1510R 5'-CCTTCYGCAGGTTCACCTAC-3' (Amaral-Zettler, L. A., McCliment, E. A., Ducklow, H. W., & Huse, S. M. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLOS ONE, 4(7), e6372. https://doi.org/10.1371/journal.pone.0006372)", library_construction_protocol = "TruSeq PCR-free"
V9 metabarcoding data, in mixed orientation. Data to publication:  target_gene = "18S", target_subfragment = "V9", nucleic_acid_extraction = "combined method from Armbrecht, L., Herrando-Pérez, S., Eisenhofer, R., Hallegraeff, G. M., Bolch, C. J. S., & Cooper, A. (2020). An optimized method for the extraction of ancient eukaryote DNA from marine sediments. Molecular Ecology Resources, 20(4), 906–919. https://doi.org/10.1111/1755-0998.13162", pcr_conditions = "initial denaturation:95degC_5min;34x denaturation:95degC_30s;annealing:57degC_45s;elongation:72degC_45s;final extension:72degC_5min",  pcr_primers = "1389F 5'-TTGTACACACCGCCC-3' and 1510R 5'-CCTTCYGCAGGTTCACCTAC-3' (Amaral-Zettler, L. A., McCliment, E. A., Ducklow, H. W., & Huse, S. M. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLOS ONE, 4(7), e6372. https://doi.org/10.1371/journal.pone.0006372)", library_construction_protocol = "TruSeq PCR-free"
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