Diagnostic microbiome profiling of native and invasive alien Drosophila species from French Guiana reveals candidate OTUs potentially linked to competitive advantages.
This study contains COI, ITS, and 16S sequencing data from Drosophila communities collected in French Guiana. Flies were sampled from four localities: Cayenne, Kaw, the Bélizon road, and the Nouragues reserve. DNA was extracted from individual fly legs, and the COI region was amplified using primers COI_F106 and COI_R154 coupled with barcode sequences at the 5' end. COI amplicons were sequenced on an Oxford Nanopore MinION MK1C device to identify individuals at the species level. Based on COI identification, flies belonging to the same species and locality were pooled for microbiome analyses. Bacterial 16S rRNA and fungal ITS regions were amplified using primers BACT27F/BACT1391R and ITS1F/ITS4KYO1, respectively. Barcode sequences were added to the 5' end of each primer, allowing multiplexing of up to 96 barcode combinations for each marker. The 16S and ITS amplicons were sequenced on MinION MK1C devices. This submission includes both individual fly samples used for COI sequencing and pooled samples used for 16S rRNA and ITS sequencing. All FASTQ files contain demultiplexed reads corresponding to a single amplicon type.
This study contains COI, ITS, and 16S sequencing data from Drosophila communities collected in French Guiana. Flies were sampled from four localities: Cayenne, Kaw, the Bélizon road, and the Nouragues reserve. DNA was extracted from individual fly legs, and the COI region was amplified using primers COI_F106 and COI_R154 coupled with barcode sequences at the 5' end. COI amplicons were sequenced on an Oxford Nanopore MinION MK1C device to identify individuals at the species level. Based on COI identification, flies belonging to the same species and locality were pooled for microbiome analyses. Bacterial 16S rRNA and fungal ITS regions were amplified using primers BACT27F/BACT1391R and ITS1F/ITS4KYO1, respectively. Barcode sequences were added to the 5' end of each primer, allowing multiplexing of up to 96 barcode combinations for each marker. The 16S and ITS amplicons were sequenced on MinION MK1C devices. This submission includes both individual fly samples used for COI sequencing and pooled samples used for 16S rRNA and ITS sequencing. All FASTQ files contain demultiplexed reads corresponding to a single amplicon type.
Institut de recherche pour le developpement