Klebsiella pnuemoniae Bacteriophage whole genomes
This study reports the complete, high-quality whole-genome sequences of two novel lytic bacteriophages, Escherichia phage Minorna and Klebsiella phage phiBO1E, targeting multidrug-resistant (MDR) members of the Enterobacteriaceae family. Escherichia phage Minorna, a member of the Autographiviridae family, possesses a streamlined ~40 kb linear dsDNA genome characterized by a highly conserved T7-like modular organization, including a signature single-subunit RNA polymerase that facilitates rapid host transcriptional hijacking. In contrast, Klebsiella phage phiBO1E exhibits a specialized ~43.8 kb genome within the Podoviridae family, specifically evolved to overcome the hypervirulent capsular barriers of K. pneumoniae. Comparative genomic analysis reveals that while both phages are obligately lytic and devoid of lysogeny-related genes (integrases/repressors), antibiotic resistance genes (ARGs), and known virulence factors, phiBO1E is distinguished by its tail-fiber-associated capsule depolymerases. These enzymes are specialized for the enzymatic degradation of K-antigen polysaccharides, providing a distinct mechanism for biofilm disruption that is absent in the more transcriptionally focused Minorna. These genomic data provide the foundational "blueprints" necessary for the development of precision-guided phage cocktails and enzybiotics targeting MDR E. coli and K. pneumoniae in clinical and environmental settings.
This study reports the complete, high-quality whole-genome sequences of two novel lytic bacteriophages, Escherichia phage Minorna and Klebsiella phage phiBO1E, targeting multidrug-resistant (MDR) members of the Enterobacteriaceae family. Escherichia phage Minorna, a member of the Autographiviridae family, possesses a streamlined ~40 kb linear dsDNA genome characterized by a highly conserved T7-like modular organization, including a signature single-subunit RNA polymerase that facilitates rapid host transcriptional hijacking. In contrast, Klebsiella phage phiBO1E exhibits a specialized ~43.8 kb genome within the Podoviridae family, specifically evolved to overcome the hypervirulent capsular barriers of K. pneumoniae. Comparative genomic analysis reveals that while both phages are obligately lytic and devoid of lysogeny-related genes (integrases/repressors), antibiotic resistance genes (ARGs), and known virulence factors, phiBO1E is distinguished by its tail-fiber-associated capsule depolymerases. These enzymes are specialized for the enzymatic degradation of K-antigen polysaccharides, providing a distinct mechanism for biofilm disruption that is absent in the more transcriptionally focused Minorna. These genomic data provide the foundational "blueprints" necessary for the development of precision-guided phage cocktails and enzybiotics targeting MDR E. coli and K. pneumoniae in clinical and environmental settings.
atmiya university