PIN1 WW domain WT is screened against a library of 3^6 variants in a Combinatorial Deep mutational scanning screen
PIN1 WW domain WT is screened against a library of 3^6 variants in a Combinatorial Deep mutational scanning screen.  The peptide library is amber codon phosphoserine (pS) encoded, with a core pSP motif, flanked by 3 amino acids on N and C terminus, making up 6 positions. The library is chosen by combinatorially permuting 3 of the top hits of PIN1 peptides, reported in this paper. https://www.nature.com/articles/nsb0800_639. These peptides are encoded within a eCPX plasmid reported in https://elifesciences.org/articles/8234.  PIN1 is biotinylated with an Avi-tag, and incubated against peptide expressed on the surface of E. Coli. with eCPX transmembrane protein. The PIN1 is then sorted for binding, and sequenced, with the raw results of this study in this study here.
PIN1 WW domain WT is screened against a library of 3^6 variants in a Combinatorial Deep mutational scanning screen.  The peptide library is amber codon phosphoserine (pS) encoded, with a core pSP motif, flanked by 3 amino acids on N and C terminus, making up 6 positions. The library is chosen by combinatorially permuting 3 of the top hits of PIN1 peptides, reported in this paper. https://www.nature.com/articles/nsb0800_639. These peptides are encoded within a eCPX plasmid reported in https://elifesciences.org/articles/8234.  PIN1 is biotinylated with an Avi-tag, and incubated against peptide expressed on the surface of E. Coli. with eCPX transmembrane protein. The PIN1 is then sorted for binding, and sequenced, with the raw results of this study in this study here.
Cancer Research UK Cambridge Institute, University of Cambridge