Transcriptional activity of XDR Salmonella Typhi after exposure to sub-MIC concentrations of antibiotics
This study investigates the transcriptomic response of an extensively drug-resistant (XDR) Salmonella enterica serovar Typhi isolate following exposure to sub-inhibitory concentrations of commonly used antibiotics in typhoid-endemic regions. Treatment groups included ampicillin (AMP), ceftriaxone (CEF), chloramphenicol (CHL), ciprofloxacin (CIP), co-trimoxazole (SXT), and tebipenem (TBP), an oral carbapenem. Mitomycin C (MMC), a DNA-damaging agent known to induce the SOS response, was included as a positive control. Total RNA was extracted from treated cultures and subjected to RNA sequencing. Reads were mapped to the reference genome of the same XDR S. Typhi isolate, and differential gene expression analysis was conducted relative to an untreated control. Functional annotation was performed using Gene Ontology (GO) and Clusters of Orthologous Groups (COG) classification systems.
This study investigates the transcriptomic response of an extensively drug-resistant (XDR) Salmonella enterica serovar Typhi isolate following exposure to sub-inhibitory concentrations of commonly used antibiotics in typhoid-endemic regions. Treatment groups included ampicillin (AMP), ceftriaxone (CEF), chloramphenicol (CHL), ciprofloxacin (CIP), co-trimoxazole (SXT), and tebipenem (TBP), an oral carbapenem. Mitomycin C (MMC), a DNA-damaging agent known to induce the SOS response, was included as a positive control. Total RNA was extracted from treated cultures and subjected to RNA sequencing. Reads were mapped to the reference genome of the same XDR S. Typhi isolate, and differential gene expression analysis was conducted relative to an untreated control. Functional annotation was performed using Gene Ontology (GO) and Clusters of Orthologous Groups (COG) classification systems.
University of Cambridge