ATAC-seq time-course profiling upon Wnt stimulation in ?CTNNB1 HEK293T cells
Wnt/ß-catenin signaling is generally thought to activate transcription primarily through pre-accessible regulatory elements. However, we previously identified cases in which ß-catenin associates with initially inaccessible chromatin regions that subsequently gain accessibility (E-MTAB-12076, Pagella et. al., (2023) Cell Systems). To determine whether stimulus-dependent chromatin opening requires ß-catenin, we performed ATAC-seq in ß-catenin knockout (?CTNNB1) human embryonic kidney 293T (HEK293T) cells, both prior to and 4 hours following pathway activation.
Wnt/ß-catenin signaling is generally thought to activate transcription primarily through pre-accessible regulatory elements. However, we previously identified cases in which ß-catenin associates with initially inaccessible chromatin regions that subsequently gain accessibility (E-MTAB-12076, Pagella et. al., (2023) Cell Systems). To determine whether stimulus-dependent chromatin opening requires ß-catenin, we performed ATAC-seq in ß-catenin knockout (?CTNNB1) human embryonic kidney 293T (HEK293T) cells, both prior to and 4 hours following pathway activation.
European Bioinformatics Institute