Guide_position_effect_on_transcript_depletion_in_CRISPR_Cas13d_system
This study is a systematic exploration of guide RNA position effect on target depletion in dual target Cas13d screens.
We are investigating if the position of the guide within the guide array, such as the order of different guides, affects how efficiently the target gets depleted in CRISPR-Cas13d system. We designed a dual gRNA CRISPR/Cas13d library, totalling over 15000 gRNAs, in order to perform a systematic screen to investigate the potential positional effect of the guide array.  In this library, we paired gRNAs targeting known Core Essential genes (CEGs) and Non-Essential genes (NEGs) with each other and with non-targeting guides in both positions. This study will produce the first systematic exploration of gRNA position effect using a dedicated large-scale dual-guide RNA library.
Wellcome Sanger Institute