Comparative non-coding small RNA profiling of extracellular vesicles from porcine oviduct fluid In vivo and oviduct epithelial cells in different in vitro models
Extracellular vesicles (EVs) derived from the oviduct are considered key mediators of the early embryo-maternal communication. The study aimed to characterize and compare the small RNA cargo of EVs derived from porcine oviduct fluid in vivo as well as porcine oviduct epithelial cell (POEC) cultures in vitro. For the in vivo samples, EVs were isolated from oviduct fluids collected around the ovulation windows (n = 4/stage): i.e., estrus (E), late estrus (LE), post ovulation (PO). Given that different culture approaches may yield divergent EV populations, we systematically compared EV samples derived from 3 different culture models: 1) Submerged 2D culture of cell line (CCLV-RIE270, n= 3). 2) Submerged 2D culture of primary POEC (n = 3). 3) Air-liquid interface (ALI, n = 5) culture of primary POEC.
Extracellular vesicles (EVs) derived from the oviduct are considered key mediators of the early embryo-maternal communication. The study aimed to characterize and compare the small RNA cargo of EVs derived from porcine oviduct fluid in vivo as well as porcine oviduct epithelial cell (POEC) cultures in vitro. For the in vivo samples, EVs were isolated from oviduct fluids collected around the ovulation windows (n = 4/stage): i.e., estrus (E), late estrus (LE), post ovulation (PO). Given that different culture approaches may yield divergent EV populations, we systematically compared EV samples derived from 3 different culture models: 1) Submerged 2D culture of cell line (CCLV-RIE270, n= 3). 2) Submerged 2D culture of primary POEC (n = 3). 3) Air-liquid interface (ALI, n = 5) culture of primary POEC.
European Bioinformatics Institute