Small RNA-seq profiling of wild-type Apilactobacillus kunkeei strains in mid-exponential phase using UMI-based libraries
This study aims to profile small RNA expression in three wild-type Apilactobacillus kunkeei (A1401, H3B2-02X and H2B1-05J) strains during mid-exponential growth. Cultures were harvested at OD 0.5–0.6, and total RNA was purified for small RNA sequencing. UMI-based small RNA libraries were generated with size selection of RNA fragments approximately 17–200 nt and sequenced using 50-bp single-end reads on the DNBSEQ platform. The dataset provides strain-specific small RNA expression profiles based on three biological replicates per strain.
This study aims to profile small RNA expression in three wild-type Apilactobacillus kunkeei (A1401, H3B2-02X and H2B1-05J) strains during mid-exponential growth. Cultures were harvested at OD 0.5–0.6, and total RNA was purified for small RNA sequencing. UMI-based small RNA libraries were generated with size selection of RNA fragments approximately 17–200 nt and sequenced using 50-bp single-end reads on the DNBSEQ platform. The dataset provides strain-specific small RNA expression profiles based on three biological replicates per strain.
European Bioinformatics Institute