NGS data for 17 reference samples using liquid biopsy
Reference cfDNA samples (Seraseq ctDNA v2 Mutation Mix, HiSS Diagnostics GmbH, Freiburg, Germany), harboring 40 known mutations at low VAFs (0.25-1.0%) were used as positive controls to explore sensitivity at different cfDNA input amounts. In total, 17 libraries generated from three reference cfDNA samples (three baseline libraries and 14 technical replicate libraries) were analyzed for bioinformatic workflow optimization and analytical performance assessment.
Reference cfDNA samples (Seraseq ctDNA v2 Mutation Mix, HiSS Diagnostics GmbH, Freiburg, Germany), harboring 40 known mutations at low VAFs (0.25-1.0%) were used as positive controls to explore sensitivity at different cfDNA input amounts. In total, 17 libraries generated from three reference cfDNA samples (three baseline libraries and 14 technical replicate libraries) were analyzed for bioinformatic workflow optimization and analytical performance assessment.
University of Lubeck