Total RNA-sequencing of liver tumours and non-tumour liver tissue from five strains of mice and rat
This study aimed to investigate transcriptional differences between liver tumours that were chemically-induced in five strains and species of mouse and rat. Male mice (Mus musculus domesticus C3H/HeOuJ and C57BL/6J, Mus musculus castaneus CAST/EiJ, and Mus Caroli CAROLI/EiJ) and rats (Fischer F344) were treated with diethylnitrosamine (DEN) to induce liver tumours. After tumour dissection, genomic DNA and RNA were simultaneously isolated and purified for library preparation and sequencing. Additional liver tissue samples were collected and processed in parallel: untreated liver from infant mice (15-day old, P15) and juvenile rats (56-day old, P56); untreated adult liver tissue; DEN-exposed adult liver tissue; spontaneous liver tumours.
This study aimed to investigate transcriptional differences between liver tumours that were chemically-induced in five strains and species of mouse and rat. Male mice (Mus musculus domesticus C3H/HeOuJ and C57BL/6J, Mus musculus castaneus CAST/EiJ, and Mus Caroli CAROLI/EiJ) and rats (Fischer F344) were treated with diethylnitrosamine (DEN) to induce liver tumours. After tumour dissection, genomic DNA and RNA were simultaneously isolated and purified for library preparation and sequencing. Additional liver tissue samples were collected and processed in parallel: untreated liver from infant mice (15-day old, P15) and juvenile rats (56-day old, P56); untreated adult liver tissue; DEN-exposed adult liver tissue; spontaneous liver tumours.
European Bioinformatics Institute