RNA-seq of murine vagina and uterus epithelial cells from ovariectomised females treated with estradiol (E2) or estetrol (E4) with different dose
To mimic menopause hormone therapy and compare the effects of estetrol (E4) to the ones of estradiol (E2), ovariectomized mice were treated for 2 weeks with E2, a physiological dose of E4 (E4low) or a supra physiological dose of E4 (E4hi). Non treated ovariectomized mice were used as control. Epithelial cells and fibroblast cells from uterus and vagina were sorted based on surface markers and RNA was collected in duplicates. RNAseq was performed for each replicate.
To mimic menopause hormone therapy and compare the effects of estetrol (E4) to the ones of estradiol (E2), ovariectomized mice were treated for 2 weeks with E2, a physiological dose of E4 (E4low) or a supra physiological dose of E4 (E4hi). Non treated ovariectomized mice were used as control. Epithelial cells and fibroblast cells from uterus and vagina were sorted based on surface markers and RNA was collected in duplicates. RNAseq was performed for each replicate.
European Bioinformatics Institute