Lake Zurich Bacterial Integrated Time Series (LZ-BITS)
This study presents a high-resolution metagenomic time series from Lake Zurich, Switzerland, comprising 37 metagenomes collected between 2 March and 4 June 2021. Sampling targeted the epilimnion (2–8 m depth) and involved filtration of 30 L lake water through a 5 µm prefilter, followed by collection of the bacterial fraction on 0.22 µm filters. DNA was extracted and sequenced using both Oxford Nanopore Technologies and Illumina platforms, enabling long-read assembly with short-read error correction. Metagenome assembly yielded 3,150 metagenome-assembled genomes (MAGs) of > 50 % completeness and < 5 % contamination. Species-level clustering produced 495 representative genomes. These genomic resources underpin the generation of sample- and species-specific protein databases for metaproteomic analyses, with proteomic data to be published separately at PRIDE.
This study presents a high-resolution metagenomic time series from Lake Zurich, Switzerland, comprising 37 metagenomes collected between 2 March and 4 June 2021. Sampling targeted the epilimnion (2–8 m depth) and involved filtration of 30 L lake water through a 5 µm prefilter, followed by collection of the bacterial fraction on 0.22 µm filters. DNA was extracted and sequenced using both Oxford Nanopore Technologies and Illumina platforms, enabling long-read assembly with short-read error correction. Metagenome assembly yielded 3,150 metagenome-assembled genomes (MAGs) of > 50 % completeness and < 5 % contamination. Species-level clustering produced 495 representative genomes. These genomic resources underpin the generation of sample- and species-specific protein databases for metaproteomic analyses, with proteomic data to be published separately at PRIDE.
Limnological Station, University of Zurich