Transcriptome of Obama nungara in the response to different types of stress and stimulus
Live individuals of Obama nungara that were subjected to several abiotic stress experiments: visible light, UVB stress, hyperoxia, hypoxia, osmoregulation and chemotaxis in response to a food source and to a dead conspecific, plus control specimens. All samples were flash frozen in liquid nitrogen and kept <-70ºC until further processing. RNA extractions after stress experiments were performed using the TRIzol reagent (Invitrogen, USA) method following the manufacturer's instructions. Concentration of all samples was assessed by Qubit RNA BR Assay kit (Thermo Fisher Scientific). Samples were subjected to either Illumina's TruSeq Stranded mRNA library preparation kit or Truseq stranded Total RNA Library with Ribo-Zero depending on the tissue and species, and sequenced on a NovaSeq 6000 (Illumina, 2 x 150 bp) for a 6Gb coverage.
Live individuals of Obama nungara that were subjected to several abiotic stress experiments: visible light, UVB stress, hyperoxia, hypoxia, osmoregulation and chemotaxis in response to a food source and to a dead conspecific, plus control specimens. All samples were flash frozen in liquid nitrogen and kept <-70ºC until further processing. RNA extractions after stress experiments were performed using the TRIzol reagent (Invitrogen, USA) method following the manufacturer's instructions. Concentration of all samples was assessed by Qubit RNA BR Assay kit (Thermo Fisher Scientific). Samples were subjected to either Illumina's TruSeq Stranded mRNA library preparation kit or Truseq stranded Total RNA Library with Ribo-Zero depending on the tissue and species, and sequenced on a NovaSeq 6000 (Illumina, 2 x 150 bp) for a 6Gb coverage.
institute of evolutionary biology