scRNA-seq of tumor infiltrating CD11c + cells in Itgax?Ptpn6 model
This study investigates the role of dendritic cells (DCs) in anti-tumor immunity, focusing on the phosphatase SHP-1 and its regulation of signaling pathways and interactions with CD8+ T cells. Using conditional knockout mouse models and single-cell transcriptomics, we show that SHP-1 loss in conventional type 1 dendritic cells (cDC1s) and macrophages disrupts interferon responses, antigen presentation, and migratory programs, leading to impaired tumor rejection and reduced efficacy of PD-1 blockade. These findings provide mechanistic insights into dendritic cell biology within the tumor microenvironment (TME) and identify SHP-1 as a key regulator with implications for the design of DC-based cancer immunotherapies.
This study investigates the role of dendritic cells (DCs) in anti-tumor immunity, focusing on the phosphatase SHP-1 and its regulation of signaling pathways and interactions with CD8+ T cells. Using conditional knockout mouse models and single-cell transcriptomics, we show that SHP-1 loss in conventional type 1 dendritic cells (cDC1s) and macrophages disrupts interferon responses, antigen presentation, and migratory programs, leading to impaired tumor rejection and reduced efficacy of PD-1 blockade. These findings provide mechanistic insights into dendritic cell biology within the tumor microenvironment (TME) and identify SHP-1 as a key regulator with implications for the design of DC-based cancer immunotherapies.
European Bioinformatics Institute