Characterization of Rhizosphere Microbial Diversity in Male and Female Date Palm (Phoenix dactylifera L.)
Date palm (Phoenix dactylifera L.) is an important fruit crop cultivated in arid and semi-arid regions of India as well as in major date-producing regions worldwide, including the Middle East, North Africa, and parts of the United States and Mediterranean countries. As a dioecious species, date palm exhibits biological differences between male and female plants that may influence their associated rhizosphere microbiomes. This study compares the rhizosphere microbial communities associated with male and female date palm using 16S rRNA gene amplicon sequencing. To investigate these differences, rhizosphere soil samples were collected from three of each female and male plants grown under similar environmental conditions. Total genomic DNA was extracted from the soil samples follow by V3-V4 hypervariable regions of the 16S rRNA gene were amplification and sequenced using the Illumina MiSeq platform, generating paired-end reads. The resulting sequencing data were used to evaluate bacterial diversity, taxonomic composition, and community structure across samples. This dataset provides a valuable resource for examining sex-specific variations in rhizosphere microbial communities and supports further research on plant-microbe interactions and their potential applications in improving date palm cultivation and management strategies.
Date palm (Phoenix dactylifera L.) is an important fruit crop cultivated in arid and semi-arid regions of India as well as in major date-producing regions worldwide, including the Middle East, North Africa, and parts of the United States and Mediterranean countries. As a dioecious species, date palm exhibits biological differences between male and female plants that may influence their associated rhizosphere microbiomes. This study compares the rhizosphere microbial communities associated with male and female date palm using 16S rRNA gene amplicon sequencing. To investigate these differences, rhizosphere soil samples were collected from three of each female and male plants grown under similar environmental conditions. Total genomic DNA was extracted from the soil samples follow by V3-V4 hypervariable regions of the 16S rRNA gene were amplification and sequenced using the Illumina MiSeq platform, generating paired-end reads. The resulting sequencing data were used to evaluate bacterial diversity, taxonomic composition, and community structure across samples. This dataset provides a valuable resource for examining sex-specific variations in rhizosphere microbial communities and supports further research on plant-microbe interactions and their potential applications in improving date palm cultivation and management strategies.
Faculty of Science, Marwadi University, Rajkot, Gujarat, India