Whole-genome sequencing and assembly of Lactobacillus crispatus isolates
This study describes the whole-genome sequencing and assembly of multiple Lactobacillus crispatus strains (RL09, RL10, RL28, RL30, and related variants) obtained in the context of a microbiological research project. Genomic DNA extracted from pure cultures was sequenced using Oxford Nanopore Technologies long-read sequencing, and complete genome assemblies were generated. For one reference strain (RL30), a hybrid assembly approach combining long-read and short-read sequencing data was applied to improve assembly accuracy. The resulting high-quality genome assemblies provide a resource for comparative and functional genomic analyses of L. crispatus.
This study describes the whole-genome sequencing and assembly of multiple Lactobacillus crispatus strains (RL09, RL10, RL28, RL30, and related variants) obtained in the context of a microbiological research project. Genomic DNA extracted from pure cultures was sequenced using Oxford Nanopore Technologies long-read sequencing, and complete genome assemblies were generated. For one reference strain (RL30), a hybrid assembly approach combining long-read and short-read sequencing data was applied to improve assembly accuracy. The resulting high-quality genome assemblies provide a resource for comparative and functional genomic analyses of L. crispatus.
Delft University of Technology