BCR repertoire of an anti-IgLON5 disease patient
B cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from one patient with anti-IgLON5 disease. Four memory B-cell populations (NS1, SI1, NS2, and SI2) were isolated by fluorescence-activated cell sorting (FACS); SI2 corresponds to the IgLON5-specific B-cell population. Single-cell 5' V(D)J libraries were generated using the Chromium Next GEM Single Cell V(D)J Reagent Kit v3 (10x Genomics) according to the manufacturer's instructions. Paired heavy- and light-chain B-cell receptor (BCR) sequences were recovered from individual B cells.
B cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from one patient with anti-IgLON5 disease. Four memory B-cell populations (NS1, SI1, NS2, and SI2) were isolated by fluorescence-activated cell sorting (FACS); SI2 corresponds to the IgLON5-specific B-cell population. Single-cell 5' V(D)J libraries were generated using the Chromium Next GEM Single Cell V(D)J Reagent Kit v3 (10x Genomics) according to the manufacturer's instructions. Paired heavy- and light-chain B-cell receptor (BCR) sequences were recovered from individual B cells.
Centre Hospitalier Universitaire Vaudois