16S rRNA profiling of fecal microbiota in LMV mice
Fecal samples were collected at week 6 after LMV induction. Total DNA was extracted, and the gut microbiota was analyzed by 16S rRNA gene amplicon sequencing. To induce LMV, BWF1 mice were administered R848 three times per week for up to 6 weeks. Feces from six mice per group were pooled to generate two samples: one from the untreated control group and one from the R848-treated group. Total DNA was extracted using the NucleoSpin DNA Stool Kit. 16S rRNA gene amplicon sequencing was performed on an Illumina MiSeq platform by Cosmo Bio., Ltd. Raw paired-end reads were generated by Macrogen (HN00113157) using the 16S Metagenomic Sequencing Library Preparation protocol (Illumina, Part 15044223 Rev. B) and converted to FASTQ format using bcl2fastq.
Fecal samples were collected at week 6 after LMV induction. Total DNA was extracted, and the gut microbiota was analyzed by 16S rRNA gene amplicon sequencing. To induce LMV, BWF1 mice were administered R848 three times per week for up to 6 weeks. Feces from six mice per group were pooled to generate two samples: one from the untreated control group and one from the R848-treated group. Total DNA was extracted using the NucleoSpin DNA Stool Kit. 16S rRNA gene amplicon sequencing was performed on an Illumina MiSeq platform by Cosmo Bio., Ltd. Raw paired-end reads were generated by Macrogen (HN00113157) using the 16S Metagenomic Sequencing Library Preparation protocol (Illumina, Part 15044223 Rev. B) and converted to FASTQ format using bcl2fastq.
Juntendo University