RNA-seq of mouse cancerous organoid cell lines from the colon of VBPN mice and genome engineered with CRISPR
Colon from mouse with genetic background VBPN was extracted and wild-type organoid culture was created. Afterwards, the organoids were treated with 4-OHT and cancerous organoids with BPN mutations were created. Subsequently, 2 Apc and 2 Rnf43 cell lines were created via CRIPSR knock-out. Additionally, Ctnnb1 gene was edited via CRISPR base editing and the edit S33F was created. Finally, also non-nargeting conrotl cell lines for conventional knock-out (NT) and base editing (NTBE) were generated. From these cell lines RNA-seq was performed.
Colon from mouse with genetic background VBPN was extracted and wild-type organoid culture was created. Afterwards, the organoids were treated with 4-OHT and cancerous organoids with BPN mutations were created. Subsequently, 2 Apc and 2 Rnf43 cell lines were created via CRIPSR knock-out. Additionally, Ctnnb1 gene was edited via CRISPR base editing and the edit S33F was created. Finally, also non-nargeting conrotl cell lines for conventional knock-out (NT) and base editing (NTBE) were generated. From these cell lines RNA-seq was performed.
European Bioinformatics Institute