RNA-sequencing of transgenic Arabidopsis seedlings constitutively expressing a Colletotrichum higginsianum effector
The fungal pathogen Colletotrichum higginsianum secretes effector proteins during infection of Arabidopsis. To explore the host processes perturbed by the effector candidate ChEC108 in host plants, transgenic Arabidopsis lines were generated to express AtUBQ10pro:ChEC108-eGFP or AtUBQ10pro:ChEC108^D164R-eGFP in the Col-0 background. D164R refers to a mutation that prevents binding of ChEC108 to the Arabidopsis protein, HIPP6. Three replicate samples (A, B, C) were collected per genotype (Col-0, AtUBQ10pro:ChEC108-eGFP or AtUBQ10pro:ChEC108^D164R-eGFP). Total RNA was extracted from 19-day-old whole seedlings grown on sterile media.
The fungal pathogen Colletotrichum higginsianum secretes effector proteins during infection of Arabidopsis. To explore the host processes perturbed by the effector candidate ChEC108 in host plants, transgenic Arabidopsis lines were generated to express AtUBQ10pro:ChEC108-eGFP or AtUBQ10pro:ChEC108^D164R-eGFP in the Col-0 background. D164R refers to a mutation that prevents binding of ChEC108 to the Arabidopsis protein, HIPP6. Three replicate samples (A, B, C) were collected per genotype (Col-0, AtUBQ10pro:ChEC108-eGFP or AtUBQ10pro:ChEC108^D164R-eGFP). Total RNA was extracted from 19-day-old whole seedlings grown on sterile media.
European Bioinformatics Institute