Cardamom
Analysis of bacteria from nasopharyngeal and gut colonization and invasive infection in infants to establish causal links in the microbiome dynamics is important for understanding the system and to accurately predict responses to therapeutic interventions. The CARDAMOM project conducted out-patient based bacterial colonisation studies and in-patient based invasive bacterial infection surveillance studies in infants for years in 2021-2022 at Angkor Hospital for Children, Siem Reap, Cambodia. Samples were cultivated on non-selective media and bacterial DNA was extracted from a plate sweep of each nasopharyngeal and gut swab. Bacterial DNA extracted from the plate sweeps will be sequenced en masse and the bacterial clones present in the sample will be detected and assembled using high-resolution metagenomic binning methods developed at Wellcome Sanger Institute. The collective target microbiome will be analysed to estimate the colonization dynamics and to predict responses to therapeutic interventions. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Analysis of bacteria from nasopharyngeal and gut colonization and invasive infection in infants to establish causal links in the microbiome dynamics is important for understanding the system and to accurately predict responses to therapeutic interventions. The CARDAMOM project conducted out-patient based bacterial colonisation studies and in-patient based invasive bacterial infection surveillance studies in infants for years in 2021-2022 at Angkor Hospital for Children, Siem Reap, Cambodia. Samples were cultivated on non-selective media and bacterial DNA was extracted from a plate sweep of each nasopharyngeal and gut swab. Bacterial DNA extracted from the plate sweeps will be sequenced en masse and the bacterial clones present in the sample will be detected and assembled using high-resolution metagenomic binning methods developed at Wellcome Sanger Institute. The collective target microbiome will be analysed to estimate the colonization dynamics and to predict responses to therapeutic interventions.
Wellcome Sanger Institute