Transcriptomic responses of zebrafish embryos to sublethal exposure to GenX (HFPO-DA): A 96-Hour RNA-seq Study
GenX (HFPO-DA) is a per- and polyfluoroalkyl substance (PFAS) increasingly used as a replacement for legacy compounds such as PFOA, but its environmental persistence and potential toxicity raise concerns. Traditional chemical risk assessment is time- and resource-intensive and provides limited insight into underlying mechanisms. In this study, zebrafish embryos were exposed to sublethal concentrations of GenX for 96 hours, and RNA sequencing was used to assess concentration-dependent changes in gene expression. These data allow identification of sensitive transcriptional responses and affected biological pathways, supporting the use of transcriptomic approaches for mechanistic, 3R-compliant evaluation of GenX toxicity.
GenX (HFPO-DA) is a per- and polyfluoroalkyl substance (PFAS) increasingly used as a replacement for legacy compounds such as PFOA, but its environmental persistence and potential toxicity raise concerns. Traditional chemical risk assessment is time- and resource-intensive and provides limited insight into underlying mechanisms. In this study, zebrafish embryos were exposed to sublethal concentrations of GenX for 96 hours, and RNA sequencing was used to assess concentration-dependent changes in gene expression. These data allow identification of sensitive transcriptional responses and affected biological pathways, supporting the use of transcriptomic approaches for mechanistic, 3R-compliant evaluation of GenX toxicity.
European Bioinformatics Institute