Genetic stability of experimental vaccine „ASFV-G-?I177L“ in adult breeding boars
This study investigated the safety of intramuscular immunization with „ASFV-G-?I177L“ in adult breeding boars. Eight healthy boars were vaccinated and monitored for clinical manifestation, viral genome and infectious virus in blood, oral fluid, semen, and tissues. Viral genome was detected in blood, oral fluid, and semen of all animals, with higher loads in succumbed individuals. Infectious „ASFV-G-?I177L“ was found in reproductive tissues, spleens, salivary glands, and semen samples from acutely affected boars and one recovered boar. Viral genomes were sequenced from blood, spleen, and the experimental vaccine.
This study investigated the safety of intramuscular immunization with „ASFV-G-?I177L“ in adult breeding boars. Eight healthy boars were vaccinated and monitored for clinical manifestation, viral genome and infectious virus in blood, oral fluid, semen, and tissues. Viral genome was detected in blood, oral fluid, and semen of all animals, with higher loads in succumbed individuals. Infectious „ASFV-G-?I177L“ was found in reproductive tissues, spleens, salivary glands, and semen samples from acutely affected boars and one recovered boar. Viral genomes were sequenced from blood, spleen, and the experimental vaccine.
Friedrich-Loeffler-Institut