Ileal 16S rRNA gene amplicon sequencing of laying hens exposed to early-life stress
This study investigated whether early-life stress during different developmental windows induces persistent changes in the ileal microbiota of commercial laying hens. The dataset was generated as part of an omics sub-study examining gut-brain-axis responses to early-life disruption in H&N Super Nick laying hens. Three coarse-mash treatment groups were included: Control hens with no early disruption, hens exposed to environmental disruption during weeks 1–3 of age, and hens exposed to environmental disruption during weeks 5–7 of age. Ileal content was collected at 35 weeks of age from 48 hens, with 16 animals per treatment group.  DNA was extracted from ileal contents and bacterial community composition was assessed by 16S rRNA gene amplicon sequencing targeting the V5–V6 region using primers V5F and V6R. Sequencing was performed using paired-end 2 × 250 bp reads. These raw sequence reads support the analysis of treatment-associated changes in ileal microbial community structure and their integration with tissue-resolved metabolomic profiles from brain, ileum and plasma. The dataset provides raw demultiplexed amplicon reads for investigating long-term microbiota responses to early-life stress in laying hens.
This study investigated whether early-life stress during different developmental windows induces persistent changes in the ileal microbiota of commercial laying hens. The dataset was generated as part of an omics sub-study examining gut-brain-axis responses to early-life disruption in H&N Super Nick laying hens. Three coarse-mash treatment groups were included: Control hens with no early disruption, hens exposed to environmental disruption during weeks 1–3 of age, and hens exposed to environmental disruption during weeks 5–7 of age. Ileal content was collected at 35 weeks of age from 48 hens, with 16 animals per treatment group.  DNA was extracted from ileal contents and bacterial community composition was assessed by 16S rRNA gene amplicon sequencing targeting the V5–V6 region using primers V5F and V6R. Sequencing was performed using paired-end 2 × 250 bp reads. These raw sequence reads support the analysis of treatment-associated changes in ileal microbial community structure and their integration with tissue-resolved metabolomic profiles from brain, ileum and plasma. The dataset provides raw demultiplexed amplicon reads for investigating long-term microbiota responses to early-life stress in laying hens.
WAGENINGEN UNIVERSITY