Single cell RNA-seq to investigate transcriptional profiles of thymic double negative T cells in WT, NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/-  mice
To examine transcriptional differences in thymic double negative (DN) T cells between wildtype (WT), NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- mice, single-cell RNA sequencing (scRNA-seq) was performed on fluorescence-activated cell sorted (FACS) DN T cells. The aim was to understand how genetic alterations in the NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- backgrounds influence early T cell development and oncogenic transformation at the transcriptional level.
To examine transcriptional differences in thymic double negative (DN) T cells between wildtype (WT), NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- mice, single-cell RNA sequencing (scRNA-seq) was performed on fluorescence-activated cell sorted (FACS) DN T cells. The aim was to understand how genetic alterations in the NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- backgrounds influence early T cell development and oncogenic transformation at the transcriptional level.
European Bioinformatics Institute