Crepidula fornicata mixed tissue iso-seq library
Long-read sequencing was employed by our lab to combine with Illumina short reads and improve our reference transcriptome for this species. RNA was extracted using phenol-choloroform from embryos at late organogenesis stage (2 brood sacs), head vesicle (5 brood sacs), early veliger stage (1 brood sac), late veliger stage (1 brood sac) and adult mantle tissue (1 sample). All extractions were diluted to 100ng/µL prior to being combined in equal parts. 1µg of RNA was sent to Novogene Co. Ltd. for isoform sequencing (Pacbio sequel II).
Long-read sequencing was employed by our lab to combine with Illumina short reads and improve our reference transcriptome for this species. RNA was extracted using phenol-choloroform from embryos at late organogenesis stage (2 brood sacs), head vesicle (5 brood sacs), early veliger stage (1 brood sac), late veliger stage (1 brood sac) and adult mantle tissue (1 sample). All extractions were diluted to 100ng/µL prior to being combined in equal parts. 1µg of RNA was sent to Novogene Co. Ltd. for isoform sequencing (Pacbio sequel II).
University of Aberdeen School of Biological Sciences