Illumina sequencing for Priestia megaterium WH320
Priestia megaterium WH320 was streaked in an LB-Lennox agar plate and grown overnight at 30?C. Next morning, a single colony was picked and re-streaked in an LB-Lennox agar plate and grown overnight at 30?C. Cells were collected from the plate and genomic DNA was extracted with the MasterPure Gram Positive DNA Purification Kit (Biosearch technologies). Extracted genome was then sequenced using an Illumina NextSeq 500 sequencer. Raw pair-end sequences were loaded into Geneious prime version 2025.1.2 for sequence trimming and pairing with an insert size of 500 base pairs. Paired sequence list was then exported in fastq format for analysis using breseq (Deatherage & Barrick, 2014).
Priestia megaterium WH320 was streaked in an LB-Lennox agar plate and grown overnight at 30?C. Next morning, a single colony was picked and re-streaked in an LB-Lennox agar plate and grown overnight at 30?C. Cells were collected from the plate and genomic DNA was extracted with the MasterPure Gram Positive DNA Purification Kit (Biosearch technologies). Extracted genome was then sequenced using an Illumina NextSeq 500 sequencer. Raw pair-end sequences were loaded into Geneious prime version 2025.1.2 for sequence trimming and pairing with an insert size of 500 base pairs. Paired sequence list was then exported in fastq format for analysis using breseq (Deatherage & Barrick, 2014).
Max-Planck-Institute for Evolutionary Biology