scRNA-seq of posterior brain organoid morphogen screen
To pattern novel posterior brain regions, we used a multiplexed morphogen screen with a single-cell sequencing readout to test 48 concentrations and combinations of 10 morphogens involved in brain patterning. This screen revealed the emergence of regions harboring under-represented cell types, such as medulla glycinergic neurons and cerebellum glutamatergic cells, that expanded mesencephalon and rhombencephalon models.
To pattern novel posterior brain regions, we used a multiplexed morphogen screen with a single-cell sequencing readout to test 48 concentrations and combinations of 10 morphogens involved in brain patterning. This screen revealed the emergence of regions harboring under-represented cell types, such as medulla glycinergic neurons and cerebellum glutamatergic cells, that expanded mesencephalon and rhombencephalon models.
European Bioinformatics Institute