Optimising_the_sequnecing_of_individual_hookworms
We are working towards an optimised approach to sample preservation, DNA extraction, and whole-genome sequencing for hookworm larvae. The larvae, hatched from eggs present in faeces, and microscopic and contain little DNA. Furthermore, sample collection can occur in locations around the world where hookworms are endemic, typically low and middle-income countries, and need stabilisation and preservation to ensure DNA is suitable for sequencing. This work will feed into projects aimed at understanding how hookworms respond to mass drug administration programs, the main form of prevention and treatment of hookworm infections affecting hundreds of millions of people worldwide. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute please see http://www.sanger.ac.uk/datasharing/
We are working towards an optimised approach to sample preservation, DNA extraction, and whole-genome sequencing for hookworm larvae. The larvae, hatched from eggs present in faeces, and microscopic and contain little DNA. Furthermore, sample collection can occur in locations around the world where hookworms are endemic, typically low and middle-income countries, and need stabilisation and preservation to ensure DNA is suitable for sequencing. This work will feed into projects aimed at understanding how hookworms respond to mass drug administration programs, the main form of prevention and treatment of hookworm infections affecting hundreds of millions of people worldwide.
Wellcome Sanger Institute