Binding sites of Fritillaria borealis U2AF2 expressed in HEK293T cells
The goal of the experiment is to reveal RNA binding preferences of Fritillaria borealis U2AF2 paralogues, using CLIP-seq. Coding sequences of U2AF2alpha, U2AF2beta and Env negative control were fused to a C-terminal V5 tag. Protein expression constructs were transfected in mammalian cell culture. After cross-linking, RNA-protein complexes were recovered with anti-V5 magnetic beads.
The goal of the experiment is to reveal RNA binding preferences of Fritillaria borealis U2AF2 paralogues, using CLIP-seq. Coding sequences of U2AF2alpha, U2AF2beta and Env negative control were fused to a C-terminal V5 tag. Protein expression constructs were transfected in mammalian cell culture. After cross-linking, RNA-protein complexes were recovered with anti-V5 magnetic beads.
European Bioinformatics Institute