Draft genome sequences of cultivable bacteria and yeasts from healthy Alphitobius diaperinus larvae
Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the functional roles of individual microorganisms. Here, we characterized the culturable fraction of the larval gut microbiota under stable laboratory rearing conditions — an approach that uniquely enables strain-level genomic resolution, functional experimentation, and bio-technological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were clones, revealing the dominant cultivable component. We provide the first high-quality draft genomes for five stable members of the A. diaperinus cultivable gut microbiota — Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4 — establishing a genomic reference framework validated through a polyphasic approach. Genome-scale annotation revealed distinct predicted metabolic potential, functional repertoires, and ecological strategies among cultivable members, providing a foundation for future ecological, functional, and biotechnological studies on this economically relevant pest.
Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the functional roles of individual microorganisms. Here, we characterized the culturable fraction of the larval gut microbiota under stable laboratory rearing conditions — an approach that uniquely enables strain-level genomic resolution, functional experimentation, and bio-technological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were clones, revealing the dominant cultivable component. We provide the first high-quality draft genomes for five stable members of the A. diaperinus cultivable gut microbiota — Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4 — establishing a genomic reference framework validated through a polyphasic approach. Genome-scale annotation revealed distinct predicted metabolic potential, functional repertoires, and ecological strategies among cultivable members, providing a foundation for future ecological, functional, and biotechnological studies on this economically relevant pest.
Instituto Nacional de Tecnologia Agropecuaria, Instituto de Microbiologia y Zoologia Agricola