Time-course analysis of gene expression during mouse embryonic stem cell differentiation into cardiomyocytes: impact of Npl deficiency.
This study aims to investigate the regulatory role of N-acetylneuraminate lyase (Npl) during the stepwise differentiation of embryonic stem cells (ESCs) into functional cardiomyocytes (CMs). We performed a high-throughput transcriptomic analysis comparing wild-type (WT) and Npl knockout (KO) cell lines at four critical developmental stages: undifferentiated embryonic stem cells (D0), embryoid bodies (D2), mesoderm progenitors (D4), and mature cardiomyocytes (D14). By examining the gene expression profiles across these time points, we seek to identify the molecular mechanisms and downstream signaling pathways through which Npl modulates mesoderm specification and cardiac lineage commitment. Our findings provide insights into the transcriptional landscape of early cardiac development and the potential involvement of Npl in congenital heart defects.
This study aims to investigate the regulatory role of N-acetylneuraminate lyase (Npl) during the stepwise differentiation of embryonic stem cells (ESCs) into functional cardiomyocytes (CMs). We performed a high-throughput transcriptomic analysis comparing wild-type (WT) and Npl knockout (KO) cell lines at four critical developmental stages: undifferentiated embryonic stem cells (D0), embryoid bodies (D2), mesoderm progenitors (D4), and mature cardiomyocytes (D14). By examining the gene expression profiles across these time points, we seek to identify the molecular mechanisms and downstream signaling pathways through which Npl modulates mesoderm specification and cardiac lineage commitment. Our findings provide insights into the transcriptional landscape of early cardiac development and the potential involvement of Npl in congenital heart defects.
European Bioinformatics Institute