In vitro grape marc and rapeseed
Natural additives represent an alternative to synthetic compounds used to reduce enteric methane from ruminants. In this context, the study aims to investigate the effect of the individual and combined use of grape marc and cracked rapeseed on the fermentation, gas production and microbiome structure on an in vitro system. Four treatments were used including: CON (base ration consisted of 65:35 forage to concentrate ratio), GM (30% of the forage was substituted by grape marc), RS (5% of concentrate was substituted by cracked rapeseed) and RS+GM (same substitutions than in the individual treatments). Short chain fatty acids, ammonia nitrogen, and pH were determined from incubated fluids, the proportions of carbon dioxide, methane and nitrous oxide were analyzed from gas in the bottles' headspace. The V4-V8 regions of the 16S/18S rRNA were sequenced on a MinION platform and taxonomically classified with the GTDB database.
Natural additives represent an alternative to synthetic compounds used to reduce enteric methane from ruminants. In this context, the study aims to investigate the effect of the individual and combined use of grape marc and cracked rapeseed on the fermentation, gas production and microbiome structure on an in vitro system. Four treatments were used including: CON (base ration consisted of 65:35 forage to concentrate ratio), GM (30% of the forage was substituted by grape marc), RS (5% of concentrate was substituted by cracked rapeseed) and RS+GM (same substitutions than in the individual treatments). Short chain fatty acids, ammonia nitrogen, and pH were determined from incubated fluids, the proportions of carbon dioxide, methane and nitrous oxide were analyzed from gas in the bottles' headspace. The V4-V8 regions of the 16S/18S rRNA were sequenced on a MinION platform and taxonomically classified with the GTDB database.
Norwegian University of Life Sciences