ChIP-seq analysis of LPS trained BMDMs  reveals remodeling of Histone 3 Lysine 4 tri-methylation (H3K4me3)
The aim of this study is to profile the distribution of the active histone modification H3K4me3 in BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ChIP-seq performed on fragmented chromatin immunoprecipitated with anti-H3K4me3 antibody in naive and trained BMDM from both genotypes. Inter-group comparisons revealed increased differentially enriched peaks near promoters of pro-inflammatory genes of trained WT BMDMs, where as this changes were blunted in Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact epigenetic regulation under LPS-training,
The aim of this study is to profile the distribution of the active histone modification H3K4me3 in BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ChIP-seq performed on fragmented chromatin immunoprecipitated with anti-H3K4me3 antibody in naive and trained BMDM from both genotypes. Inter-group comparisons revealed increased differentially enriched peaks near promoters of pro-inflammatory genes of trained WT BMDMs, where as this changes were blunted in Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact epigenetic regulation under LPS-training,
European Bioinformatics Institute