Loss of ptr-6 restores eggshell integrity and embryonic viability in C. elegans fatty acid synthase mutants
Fatty acid synthase (FASN) is a key rate-limited, dimeric multi-enzyme complex in the de novo lipogenesis pathway. We performed an EMS mutagenesis screen in the temperature-sensitive fasn-1(g43ts) mutant to identify mutations that suppress embryonic lethality. Each independent suppressor strain was sequenced by WGS to identify the causal mutation.
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