Arbuscular mycorrhizal fungi-mediated transcriptional changes in quinoa under salinity stress
Quinoa (Chenopodium quinoa Willd.) is globally valued for its high nutritional content and adaptability to salinity stress, however strategies to further enhance its resilience are needed to address climate challenge. While arbuscular mycorrhizal fungi (AMF) have been shown to improve quinoa's salinity tolerance, the mineral uptake and molecular responses, underlying this beneficial interaction remain poorly studied. To investigate genes involved in salinity tolerance of quinoa cv. 'Titicaca', we conducted a transcriptome analysis of leaf material from the fourth and fifth rows in three replicates for each treatment group: 50 g sterilized AMF and 50 g AMF under non-saline (0 mM NaCl) and saline (200 mM NaCl) conditions. Total RNA was extracted with the innuPREP Plant RNA Kit (Analytik Jena, Germany) following manufacturer's instructions.  Library construction and sequencing were done at Novogene (UK) Company Limited. Briefly, RNA integrity and quantitation were determined with the Agilent 2100 Bioanalyzer. Total mRNA was enriched with oligo(dT) beads and randomly fragmented with fragmentation buffer. A stranded specific library was then prepared for each sample and sequenced with a NovaSeq 6000 PE150 platform.
Quinoa (Chenopodium quinoa Willd.) is globally valued for its high nutritional content and adaptability to salinity stress, however strategies to further enhance its resilience are needed to address climate challenge. While arbuscular mycorrhizal fungi (AMF) have been shown to improve quinoa's salinity tolerance, the mineral uptake and molecular responses, underlying this beneficial interaction remain poorly studied. To investigate genes involved in salinity tolerance of quinoa cv. 'Titicaca', we conducted a transcriptome analysis of leaf material from the fourth and fifth rows in three replicates for each treatment group: 50 g sterilized AMF and 50 g AMF under non-saline (0 mM NaCl) and saline (200 mM NaCl) conditions. Total RNA was extracted with the innuPREP Plant RNA Kit (Analytik Jena, Germany) following manufacturer's instructions.  Library construction and sequencing were done at Novogene (UK) Company Limited. Briefly, RNA integrity and quantitation were determined with the Agilent 2100 Bioanalyzer. Total mRNA was enriched with oligo(dT) beads and randomly fragmented with fragmentation buffer. A stranded specific library was then prepared for each sample and sequenced with a NovaSeq 6000 PE150 platform.
Leibniz Institute of Plant Genetics and Crop Plant Research