Transportome remodeling in bone vascularization
Angiogenic process plays a pivotal role in bone regeneration, as bone tissue renewal requires both osteogenesis and vascularization. One of the biggest challenges in tissue engineering is to obtain an adequately vascularized scaffold to sustain and optimize bone regeneration, since the efficiency of the graft in terms of both bone development and long-term graft survival is strongly determined by the ability of the scaffold to support the development of vascular networks. Many studies have emphasized the complex crosstalk between endothelial cells and osteodifferentiating stem cells in bone tissue renewal, the former being required for the development of a functional vascular network, and the latter accounting for the high regenerative potential and self-renewing properties of bones. The main aim of the project is to assess in vitro the potential role of the transportome (the array of ion channels and transporters expressed in cell membranes) in the complex crosstalk between bone formation and vascular component, by investigating the transcriptional and functional profile of stem cells and vascular endothelium during osteodifferentiation.
Angiogenic process plays a pivotal role in bone regeneration, as bone tissue renewal requires both osteogenesis and vascularization. One of the biggest challenges in tissue engineering is to obtain an adequately vascularized scaffold to sustain and optimize bone regeneration, since the efficiency of the graft in terms of both bone development and long-term graft survival is strongly determined by the ability of the scaffold to support the development of vascular networks. Many studies have emphasized the complex crosstalk between endothelial cells and osteodifferentiating stem cells in bone tissue renewal, the former being required for the development of a functional vascular network, and the latter accounting for the high regenerative potential and self-renewing properties of bones. The main aim of the project is to assess in vitro the potential role of the transportome (the array of ion channels and transporters expressed in cell membranes) in the complex crosstalk between bone formation and vascular component, by investigating the transcriptional and functional profile of stem cells and vascular endothelium during osteodifferentiation.
University of Turin