The study isolated Salmonella from clinical, animal, and environmental samples
Non-Typhoidal Salmonella (NTS) is considered as a major foodborne pathogen worldwide especially in low and middle-income countries. This study used whole genome sequencing (WGS) to analyze the epidemiological trends, sequence types (STs), antimicrobial resistance (AMR), genome dynamics and to assess phylogenetic relatedness of NTS in Burkina Faso. Methods: A total of 34 presumptive Salmonella enterica from animal products (n=20), environmental sources (n=8) and diarrheal stool samples (n=6) were identified by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of this, isolates confirmed as S. enterica by MALDI-TOF MS were sequenced. The whole-genome of the isolates were sequenced and bioinformatic analysis was performed using the Bactopia pipeline.  Results: Of the 34 presumptive Salmonella isolates, 22 (64.70%) were identified as Salmonella enterica by MALDI-TOF MS. Out of 22 Salmonella enterica detected, 16 were from animal products and 3 each were from environmental and diarrheal stool samples. Characterization of the isolates showed that most (n = 5/22; 22.7%) of the isolates were S. Schwarzengrund, ST96, and S. Give, ST516 (n = 4/22; 18.18%). One S. Schwarzengrund, ST96, isolate harbored antibiotic resistance (AMR) genes whereas a S. Molade or Wippra, ST544, harbored the AMR genes. In addition, one S. Llandoff isolate carried  Virulence genes including invA, avrA, iroB, iroC and sinH gene were observed in all the isolates while 72.73% of the isolates harboured the cdtB gene.
Non-Typhoidal Salmonella (NTS) is considered as a major foodborne pathogen worldwide especially in low and middle-income countries. This study used whole genome sequencing (WGS) to analyze the epidemiological trends, sequence types (STs), antimicrobial resistance (AMR), genome dynamics and to assess phylogenetic relatedness of NTS in Burkina Faso. Methods: A total of 34 presumptive Salmonella enterica from animal products (n=20), environmental sources (n=8) and diarrheal stool samples (n=6) were identified by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of this, isolates confirmed as S. enterica by MALDI-TOF MS were sequenced. The whole-genome of the isolates were sequenced and bioinformatic analysis was performed using the Bactopia pipeline.  Results: Of the 34 presumptive Salmonella isolates, 22 (64.70%) were identified as Salmonella enterica by MALDI-TOF MS. Out of 22 Salmonella enterica detected, 16 were from animal products and 3 each were from environmental and diarrheal stool samples. Characterization of the isolates showed that most (n = 5/22; 22.7%) of the isolates were S. Schwarzengrund, ST96, and S. Give, ST516 (n = 4/22; 18.18%). One S. Schwarzengrund, ST96, isolate harbored antibiotic resistance (AMR) genes whereas a S. Molade or Wippra, ST544, harbored the AMR genes. In addition, one S. Llandoff isolate carried  Virulence genes including invA, avrA, iroB, iroC and sinH gene were observed in all the isolates while 72.73% of the isolates harboured the cdtB gene.
Centre National de la Recherche Scientifique et Technologique (CNRST)