RNA-seq analysis of LPS-trained bone marrow derived macrophages (BMDMs) reveals altered glutamine and glycolysis metabolic pathways
The aim of this study is to evaluate data by comparing transcriptome profile (RNA-seq) of BMDMs trained with low-dose LPS and untrained BMDMs. Trained immunity enables innate immune cells to acquire memory-like responses, offering a promising strategy to enhance antitumor immunity. However, the metabolic-epigenetic mechanisms underlying this process remain incompletely defined, limiting therapeutic translation. Here, we elucidate how metabolic reprogramming shapes epigenetic memory in macrophages and how this process can be harnessed to potentiate antitumor responses. The impact of LPS training in BMDMs is assessed  focusing on the changes in metabolism related genes and difference in any other categories of genes to fully comprehend the metabolic reprogramming.
The aim of this study is to evaluate data by comparing transcriptome profile (RNA-seq) of BMDMs trained with low-dose LPS and untrained BMDMs. Trained immunity enables innate immune cells to acquire memory-like responses, offering a promising strategy to enhance antitumor immunity. However, the metabolic-epigenetic mechanisms underlying this process remain incompletely defined, limiting therapeutic translation. Here, we elucidate how metabolic reprogramming shapes epigenetic memory in macrophages and how this process can be harnessed to potentiate antitumor responses. The impact of LPS training in BMDMs is assessed  focusing on the changes in metabolism related genes and difference in any other categories of genes to fully comprehend the metabolic reprogramming.
European Bioinformatics Institute