gthermoleovorans_2025
In this study, we engineered Geobacillus thermoleovorans through adaptive laboratory evolution (ALE) for robust growth on adipic acid (AA) and 1,4-butanediol (BDO), two relevant monomers for example derived from the biodegradable polymer PBAT. Both evolved strains were characterized for substrate consumption and growth. Furtehrmore, the genomes of both the wild-type and evolved strains were sequenced to obtain (a) a high quality reference genome and (b) to check for genomic mutations. Also,  gene expression levels were determined for the different strains when grown on the respective substrate compared to glucose.
In this study, we engineered Geobacillus thermoleovorans through adaptive laboratory evolution (ALE) for robust growth on adipic acid (AA) and 1,4-butanediol (BDO), two relevant monomers for example derived from the biodegradable polymer PBAT. Both evolved strains were characterized for substrate consumption and growth. Furtehrmore, the genomes of both the wild-type and evolved strains were sequenced to obtain (a) a high quality reference genome and (b) to check for genomic mutations. Also,  gene expression levels were determined for the different strains when grown on the respective substrate compared to glucose.
Forschungszentrum Juelich GmbH