Chemical preservation methods for wastewater resistome surveillance by shotgun metagenomics
This study evaluates chemical preservation methods for maintaining the integrity of the wastewater resistome prior to shotgun metagenomic sequencing. Influent wastewater samples were preserved using different chemical additives and storage conditions, then subjected to DNA extraction and short-read metagenomic sequencing. Sequencing reads were analysed to characterise antimicrobial resistance genes, mobile genetic elements, and microbial community composition, in order to identify preservation approaches that best retain resistome and taxonomic signals for environmental antimicrobial resistance surveillance.
This study evaluates chemical preservation methods for maintaining the integrity of the wastewater resistome prior to shotgun metagenomic sequencing. Influent wastewater samples were preserved using different chemical additives and storage conditions, then subjected to DNA extraction and short-read metagenomic sequencing. Sequencing reads were analysed to characterise antimicrobial resistance genes, mobile genetic elements, and microbial community composition, in order to identify preservation approaches that best retain resistome and taxonomic signals for environmental antimicrobial resistance surveillance.
https://www.ebi.ac.uk/ena/submit/webin