Strongyloides sp. Associated with Wild Boar Faecal Samples from Bangladesh
This study contains sequencing reads generated from Strongyloides sp. recovered from faecal samples collected from wild boar hosts in Bangladesh. The samples, corresponding to host IDs BDD503 and BDG902, were investigated as part of a broader project examining the genetic diversity, host associations, and potential zoonotic transmission of Strongyloides parasites (https://www.pnas.org/doi/10.1073/pnas.2424630122 & https://livrepository.liverpool.ac.uk/3185184/). Host identity was inferred from faecal-derived mitochondrial 12S and 16S rRNA sequences, which supported assignment of the hosts as wild boar.  Initial taxonomic assessment of the parasite-derived reads indicated that the worms associated with BDD503 and BDG902 could not be confidently assigned to currently available Strongyloides reference genomes using either nuclear or mitochondrial genomic data. These samples therefore appear to represent unidentified Strongyloides lineages associated with wild boar. To further investigate their identity, mitochondrial genome reconstruction was attempted using a reference-guided read extraction and de novo assembly approach. Briefly, Strongyloides-like reads were first identified, followed by de novo assembly using SPAdes. Although this approach recovered several short mitochondrial-like contigs, complete mitochondrial genomes could not be reconstructed from the available data.  The sequencing reads deposited here are therefore provided as a resource for future comparative genomic, phylogenetic, and taxonomic studies of Strongyloides parasites from wildlife hosts.
This study contains sequencing reads generated from Strongyloides sp. recovered from faecal samples collected from wild boar hosts in Bangladesh. The samples, corresponding to host IDs BDD503 and BDG902, were investigated as part of a broader project examining the genetic diversity, host associations, and potential zoonotic transmission of Strongyloides parasites (https://www.pnas.org/doi/10.1073/pnas.2424630122 & https://livrepository.liverpool.ac.uk/3185184/). Host identity was inferred from faecal-derived mitochondrial 12S and 16S rRNA sequences, which supported assignment of the hosts as wild boar.  Initial taxonomic assessment of the parasite-derived reads indicated that the worms associated with BDD503 and BDG902 could not be confidently assigned to currently available Strongyloides reference genomes using either nuclear or mitochondrial genomic data. These samples therefore appear to represent unidentified Strongyloides lineages associated with wild boar. To further investigate their identity, mitochondrial genome reconstruction was attempted using a reference-guided read extraction and de novo assembly approach. Briefly, Strongyloides-like reads were first identified, followed by de novo assembly using SPAdes. Although this approach recovered several short mitochondrial-like contigs, complete mitochondrial genomes could not be reconstructed from the available data.  The sequencing reads deposited here are therefore provided as a resource for future comparative genomic, phylogenetic, and taxonomic studies of Strongyloides parasites from wildlife hosts.
University of Liverpool