Transcriptome comparisons of dimorphic Arthrostemma ciliatum stamen whorls at different developmental stages to find candidate genes causing heteranthery
The experiment was performed to find differentially expressed transcripts/genes between the two stamen types responsible for causing the observed morphological differences. Four different developmental stages suitable for comparisons were identified based on morphological characteristics and flower bud sizes. The two stamen whorls at each of these developmental stages were dissected from multiple flower buds and RNA was extracted from each stamen whorl separately. Three such biological replicates per stamen whorl at the four developmental stages (i.e 3x2x4 =24 samples) and three additional leaf samples (biological replicates) were sequenced (mRNA library prep, Illumina seq.-paired end, 9G Raw data).
The experiment was performed to find differentially expressed transcripts/genes between the two stamen types responsible for causing the observed morphological differences. Four different developmental stages suitable for comparisons were identified based on morphological characteristics and flower bud sizes. The two stamen whorls at each of these developmental stages were dissected from multiple flower buds and RNA was extracted from each stamen whorl separately. Three such biological replicates per stamen whorl at the four developmental stages (i.e 3x2x4 =24 samples) and three additional leaf samples (biological replicates) were sequenced (mRNA library prep, Illumina seq.-paired end, 9G Raw data).
European Bioinformatics Institute