Genomic Characterization of Monkeypox Virus from the 2022 Croatian Outbreak
Clinical swabs from rectal, oropharyngeal, and skin lesions of 17 MPXV-positive patients were analyzed using MPXV-specific qPCR. Viral isolation was performed using Vero E6 cell cultures, followed by whole-genome sequencing on the Illumina MiSeq platform. MPXV genomes were obtained and assembled from 12 PCR-positive patient samples collected between July 2022 and January 2023. The resulting genome coverage was 93.5% to 96.7%.
Clinical swabs from rectal, oropharyngeal, and skin lesions of 17 MPXV-positive patients were analyzed using MPXV-specific qPCR. Viral isolation was performed using Vero E6 cell cultures, followed by whole-genome sequencing on the Illumina MiSeq platform. MPXV genomes were obtained and assembled from 12 PCR-positive patient samples collected between July 2022 and January 2023. The resulting genome coverage was 93.5% to 96.7%.
University hospital for infectious diseases dr. fran mihaljevic