Transcriptome of human aortic smooth muscle cells is dependent on mechanical stretch and substrate coating
To investigate how mechanical stretch influences smooth muscle phenotype, we cultured human aortic smooth muscle cells (SMCs) on soft silicon membranes coated with collagen-I (COL1), fibronectin (FIB), or laminin (LAM). SMCs were exposed to physiological (10%) stretch, pathological (15%) stretch, or no stretch for 6 hours, followed by RNA extraction and sequencing (RNA-seq).
To investigate how mechanical stretch influences smooth muscle phenotype, we cultured human aortic smooth muscle cells (SMCs) on soft silicon membranes coated with collagen-I (COL1), fibronectin (FIB), or laminin (LAM). SMCs were exposed to physiological (10%) stretch, pathological (15%) stretch, or no stretch for 6 hours, followed by RNA extraction and sequencing (RNA-seq).
European Bioinformatics Institute