PCR amplicon sequencing targeting major bacterial genera from honeybee microbiota on Apis mellifera from Clermont-Ferrand, Apis mellifera unicolor from La Réunion and Bombus terrestris from Clermont-Ferrand
21 primer couples designed to target 8 major taxa from honeybee microbiota have been tested out for specificity by amplicon sequencing via Nanopore MINion technology ("pool" samples). Two universal bacterial primers has also been tested (BAC_A and BAC_B). Amplification reaction were made on 3 biological matrix : One extract, named "Am_C", was a pool of ten DNA extracts sampled in France by Blot et al. (2019) and mostly belonging to the European C lineage. Another sample, named "Am_A", was a pool of 129 DNA extracts from Apis mellifera unicolor workers belonging to the African A lineage and sampled in La Réunion island by Jobart et al., (2024). A third sample, identified as "Bte", was the DNA extracted (DNeasy Blood and Tissue kit, QIAGEN) from four abdomens of Bombus terrestris sampled in France in 2025.
21 primer couples designed to target 8 major taxa from honeybee microbiota have been tested out for specificity by amplicon sequencing via Nanopore MINion technology ("pool" samples). Two universal bacterial primers has also been tested (BAC_A and BAC_B). Amplification reaction were made on 3 biological matrix : One extract, named "Am_C", was a pool of ten DNA extracts sampled in France by Blot et al. (2019) and mostly belonging to the European C lineage. Another sample, named "Am_A", was a pool of 129 DNA extracts from Apis mellifera unicolor workers belonging to the African A lineage and sampled in La Réunion island by Jobart et al., (2024). A third sample, identified as "Bte", was the DNA extracted (DNeasy Blood and Tissue kit, QIAGEN) from four abdomens of Bombus terrestris sampled in France in 2025.
Centre National de la Recherche Scientifique / Universite Clermont Auvergne