RNA-seq of murine mammary epithelial cells from ovariectomised females treated with estradiol (E2) or estetrol (E4)
To mimick menopause hormone therapy and compare the effects of estetrol (E4) to the ones of estradiol (E2), ovariectomized mice were treated for 2 weeks with E2, a physiological dose of E4 (E4low) or a supra physiological dose of E4 (E4hi). Non treated ovariectomized mice were used as control. Basal cells, estrogen positive luminal cells and estrogen negative luminal cells from MG were sorted based on surface markers and RNA was collected in duplicates, except for the E4hi condition which was collected in triplicates. RNAseq was performed for each replicate.
To mimick menopause hormone therapy and compare the effects of estetrol (E4) to the ones of estradiol (E2), ovariectomized mice were treated for 2 weeks with E2, a physiological dose of E4 (E4low) or a supra physiological dose of E4 (E4hi). Non treated ovariectomized mice were used as control. Basal cells, estrogen positive luminal cells and estrogen negative luminal cells from MG were sorted based on surface markers and RNA was collected in duplicates, except for the E4hi condition which was collected in triplicates. RNAseq was performed for each replicate.
European Bioinformatics Institute