Transcriptomic and genomic analysis of Rev3l mutant mouse embryonic fibroblasts
This study investigates the role of DNA polymerase ? catalytic subunit Rev3l in primary mouse embryonic fibroblasts (MEFs). We performed RNA sequencing (RNA-Seq) to identify differentially expressed genes between wild-type (Rev3lWT/WT) and the mutant (Rev3lDG-AA/DG-AA) cells. Additionally, whole genome sequencing (WGS) was conducted to characterize mutational signatures and genomic variants in WT and and TASP1 KO cells before and after four cycles of cisplatin (0.8ug/ml). Datasets from RNAseq (PE-100bp) were generated using Illumina NextSeq 2000 while datasets form WGS were generated from BGISEQ-500.
This study investigates the role of DNA polymerase ? catalytic subunit Rev3l in primary mouse embryonic fibroblasts (MEFs). We performed RNA sequencing (RNA-Seq) to identify differentially expressed genes between wild-type (Rev3lWT/WT) and the mutant (Rev3lDG-AA/DG-AA) cells. Additionally, whole genome sequencing (WGS) was conducted to characterize mutational signatures and genomic variants in WT and and TASP1 KO cells before and after four cycles of cisplatin (0.8ug/ml). Datasets from RNAseq (PE-100bp) were generated using Illumina NextSeq 2000 while datasets form WGS were generated from BGISEQ-500.
Gustave Roussy, Centre national de la recherche scientifique - Unites Mixtes de Recherche 9019