Genome skimming of cultured marine nematodes and metagenomics analysis of mock communities
In this study, we generated genome-scale reference data for free-living marine nematodes grown on monospecific culture plates using low-coverage whole-genome sequencing (genome skimming). The resulting genome skims were used to assemble mitochondrial genomes. DNA was extracted from communities assembled with individuals from the culture plates and shotgun sequenced with both Illumina and Nanopore. The resulting reads were then classified against the reference data generated.
In this study, we generated genome-scale reference data for free-living marine nematodes grown on monospecific culture plates using low-coverage whole-genome sequencing (genome skimming). The resulting genome skims were used to assemble mitochondrial genomes. DNA was extracted from communities assembled with individuals from the culture plates and shotgun sequenced with both Illumina and Nanopore. The resulting reads were then classified against the reference data generated.
Flanders Research Institute for Agriculture, Fisheries and Food