Epigenetic changes after sperm cryopreservation in common carp and consequences for the offspring
This project aims to characterize DNA methylation changes in carp spermatozoa after cryopreservation and explore whether cryopreserved spermatozoa transmit epigenetic alterations to their progeny. Each sperm sample from a given male was split into two groups—one fresh and one cryopreserved—ensuring each male had both a fresh and a cryopreserved counterpart (paired samples). Fresh samples were stored overnight in a dilution medium for preservation and cryopreserved samples were cryopreserved in a solution containing 10% DMSO. Both groups of samples were used to produce embryos sired with fresh or cryopreserved sperm. Following successful DNA extraction from both sperm and embryos, sperm samples were sent to NOVOGENE for sequencing (150 bp and 94Gbases). The initial sequencing depth will be 28X (analyze each DNA strand separately), which will be reduced to 14X after trimming. Bioinformatic analysis of the sequencing data will show the effect of cryopreservation in common carp sperm. Embryos were sent for GetPlage (we used only 8 males)
This project aims to characterize DNA methylation changes in carp spermatozoa after cryopreservation and explore whether cryopreserved spermatozoa transmit epigenetic alterations to their progeny. Each sperm sample from a given male was split into two groups—one fresh and one cryopreserved—ensuring each male had both a fresh and a cryopreserved counterpart (paired samples). Fresh samples were stored overnight in a dilution medium for preservation and cryopreserved samples were cryopreserved in a solution containing 10% DMSO. Both groups of samples were used to produce embryos sired with fresh or cryopreserved sperm. Following successful DNA extraction from both sperm and embryos, sperm samples were sent to NOVOGENE for sequencing (150 bp and 94Gbases). The initial sequencing depth will be 28X (analyze each DNA strand separately), which will be reduced to 14X after trimming. Bioinformatic analysis of the sequencing data will show the effect of cryopreservation in common carp sperm. Embryos were sent for GetPlage (we used only 8 males)
INRAE