Comparative transcriptomics of Anabaena sp. PCC7120 grown as biofilm under bubbling or their concurrent planktonic fractions
Bacterial biofilms are microbial communities that grow attached to a surface, embedded in a complex matrix composed of extracellular polymeric compounds such as exopolysaccharides. They are commonly induced in response to stresses such as desiccation or depredation, and display increased resilience. Model cyanobacterium Anabaena sp. PCC7120, typically cultured planktonically, is able to form biofilms. In order to advance in the understanding of phototrophic biofilm formation, we carried out comparative transcriptomic analysis of biofilm and planktonic Anabaena and observed vast alterations. A total of 1099 differentially expressed genes (DEGs) were identified, of which 641 could be assigned a functional annotation. Major central metabolic pathways were affected, including carbohydrate metabolism, photosynthesis, ribosome biogenesis andtranslation. nitrogen metabolism and cellular envelops maintenance, among others.
Bacterial biofilms are microbial communities that grow attached to a surface, embedded in a complex matrix composed of extracellular polymeric compounds such as exopolysaccharides. They are commonly induced in response to stresses such as desiccation or depredation, and display increased resilience. Model cyanobacterium Anabaena sp. PCC7120, typically cultured planktonically, is able to form biofilms. In order to advance in the understanding of phototrophic biofilm formation, we carried out comparative transcriptomic analysis of biofilm and planktonic Anabaena and observed vast alterations. A total of 1099 differentially expressed genes (DEGs) were identified, of which 641 could be assigned a functional annotation. Major central metabolic pathways were affected, including carbohydrate metabolism, photosynthesis, ribosome biogenesis andtranslation. nitrogen metabolism and cellular envelops maintenance, among others.
European Bioinformatics Institute