DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus
This study aimed to characterize the global genomic diversity and evolutionary relationships of Ostreid herpesvirus type 1 (OsHV-1) infecting the Pacific oyster Magallana gigas using whole-genome sequencing. A total of 274 naturally infected oysters collected between 1993 and 2022 from several oyster-producing countries including France, USA, Spain, Germany, Ireland, Korea, Australia and New Zealand were analyzed, with the majority of samples originating from multiple French coastal production areas. Viral DNA extracted from oyster mantle tissue was used to generate Illumina DNA-seq libraries, and genomes were sequenced using high-throughput Illumina short-read technology across several sequencing platforms. Sequencing reads were quality-filtered and assembled de novo to reconstruct complete OsHV-1 genomes for comparative genomic and phylogenomic analyses.
This study aimed to characterize the global genomic diversity and evolutionary relationships of Ostreid herpesvirus type 1 (OsHV-1) infecting the Pacific oyster Magallana gigas using whole-genome sequencing. A total of 274 naturally infected oysters collected between 1993 and 2022 from several oyster-producing countries including France, USA, Spain, Germany, Ireland, Korea, Australia and New Zealand were analyzed, with the majority of samples originating from multiple French coastal production areas. Viral DNA extracted from oyster mantle tissue was used to generate Illumina DNA-seq libraries, and genomes were sequenced using high-throughput Illumina short-read technology across several sequencing platforms. Sequencing reads were quality-filtered and assembled de novo to reconstruct complete OsHV-1 genomes for comparative genomic and phylogenomic analyses.
French Research Institute for Exploitation of the Sea