16S RNA amplicon sequencing of leaf microbiomes of high-productive versus drought-tolerant varieties of maize and wheat grown in controlled-plant chamber under drought stress
Partial 16S rRNA (V4-V6 SSU region) encoding gene were PCR amplified from phyllosphere epiphytes extracted from the maize and wheat grown in plant chambers under controlled conditions at two relative moisture: i.e. water shortage stress (20% moisture) and no stress (60% moisture). Amplicon were sequences using the Illumina MiSeq paired-end (3x300bp). Biological triplicate sampling was conducted at the juvenile (15 days), vegetative (45 days), and reproductive (60 days) stages for each crops and variety using high-productive crop varieties (maize Kanchan 25, code K25-05; wheat Pusa Tejas code PT) compared to drought-tolerant crop varieties (maize P(3502) code P; wheat Pusa Ujala code PU).
Partial 16S rRNA (V4-V6 SSU region) encoding gene were PCR amplified from phyllosphere epiphytes extracted from the maize and wheat grown in plant chambers under controlled conditions at two relative moisture: i.e. water shortage stress (20% moisture) and no stress (60% moisture). Amplicon were sequences using the Illumina MiSeq paired-end (3x300bp). Biological triplicate sampling was conducted at the juvenile (15 days), vegetative (45 days), and reproductive (60 days) stages for each crops and variety using high-productive crop varieties (maize Kanchan 25, code K25-05; wheat Pusa Tejas code PT) compared to drought-tolerant crop varieties (maize P(3502) code P; wheat Pusa Ujala code PU).
Centre National de la Recherche Scientifique - Strasbourg University