HVS transcriptome
Herpesvirus saimiri (HVS) is a gamma-1-herpesvirus of squirrel monkeys. Its geneome seqeunce is well established but its transcriptome has not been characterised. Here we have infected Owl Monkey kidney (OMK) cells – which are permissive for HVS – with HVS generated from recombinant BAC clone of HVS-A11-S4 engineered with a luciferase-mNeonGreen expression cassette. The HVS-A11-S4 strain contains a deletion of a SacI restriction fragment that results in the deletion of the ORF1 and ORF2 oncogenes. and some ncRNAs. RNA was harvested at specified times after infection and sequenced via Nanopore direct RNA seqeuncing.
Herpesvirus saimiri (HVS) is a gamma-1-herpesvirus of squirrel monkeys. Its geneome seqeunce is well established but its transcriptome has not been characterised. Here we have infected Owl Monkey kidney (OMK) cells – which are permissive for HVS – with HVS generated from recombinant BAC clone of HVS-A11-S4 engineered with a luciferase-mNeonGreen expression cassette. The HVS-A11-S4 strain contains a deletion of a SacI restriction fragment that results in the deletion of the ORF1 and ORF2 oncogenes. and some ncRNAs. RNA was harvested at specified times after infection and sequenced via Nanopore direct RNA seqeuncing.
Section of Virology, Dept of Infectious disease,