QTLseq and QTLmapping reveal genomic regions for beet mild yellowing virus resistance and susceptibility in wild beet Ã— sugar beet populations
Beet mild yellowing virus (BMYV) is one among the poleroviruses, which cause virus yellows (VY) disease in sugar beet. The virus is transmitted by aphids and infections may result in significant yield losses. With the new restrictions in the use of neonicotinoids for managing aphid vectors, there is a high demand for alternative and sustainable methods to control VY. Developing cultivars of sugar beet with resistance or tolerance to BMYV could help in reducing crop losses and avoiding the dependency on insecticides. Hence, we performed QTLseq (Quantitative trait loci sequencing) and QTL (Quantitative trait loci) mapping studies in mapping populations obtained by crossing a wild beet genotype resistant to BMYV with a susceptible cultivar of sugar beet. For QTLseq, disease scoring was carried out using BMYV-inoculated plants in the F2 generation and for QTL mapping, the virus content in leaves and roots was quantified in plants of the S2 population.
Beet mild yellowing virus (BMYV) is one among the poleroviruses, which cause virus yellows (VY) disease in sugar beet. The virus is transmitted by aphids and infections may result in significant yield losses. With the new restrictions in the use of neonicotinoids for managing aphid vectors, there is a high demand for alternative and sustainable methods to control VY. Developing cultivars of sugar beet with resistance or tolerance to BMYV could help in reducing crop losses and avoiding the dependency on insecticides. Hence, we performed QTLseq (Quantitative trait loci sequencing) and QTL (Quantitative trait loci) mapping studies in mapping populations obtained by crossing a wild beet genotype resistant to BMYV with a susceptible cultivar of sugar beet. For QTLseq, disease scoring was carried out using BMYV-inoculated plants in the F2 generation and for QTL mapping, the virus content in leaves and roots was quantified in plants of the S2 population.
Swedish University of Agricultural Sciences