Increased sensitivity of FluoroType MTBDR v2 with addition of IS6110 analysis for detection of Mycobacterium tuberculosis complex
Prospective head-to-head evaluation of two versions of the FluoroType MTBDR assay for detection of Mycobacterium tuberculosis complex and resistance-associated mutations. Clinical pulmonary and extrapulmonary specimens were analysed using the previous FluoroType MTBDR v2 assay and an updated version including the IS6110 insertion sequence target. Culture and whole-genome sequencing were used as reference methods. The study assessed the effect of IS6110 inclusion on diagnostic sensitivity and specificity, particularly in paucibacillary and microscopy-negative specimens.
Prospective head-to-head evaluation of two versions of the FluoroType MTBDR assay for detection of Mycobacterium tuberculosis complex and resistance-associated mutations. Clinical pulmonary and extrapulmonary specimens were analysed using the previous FluoroType MTBDR v2 assay and an updated version including the IS6110 insertion sequence target. Culture and whole-genome sequencing were used as reference methods. The study assessed the effect of IS6110 inclusion on diagnostic sensitivity and specificity, particularly in paucibacillary and microscopy-negative specimens.
STATENS SERUM INSTITUT