RNA-seq analysis of HAstV1, HAstV4, MLB1, MLB2 and VA1 astrovirus infection in cell culture
The purpose of the study was to investigate the dynamics of viral RNA production during astrovirus infection. Caco-2 cells were infected at MOI 5 with HAstV1, HAstV4 or VA1; Huh7.5.1 cells were infected at MOI 5 with MLB1 or MLB2. Cells were harvested at 6, 12, 18, 24 (HAstV1) or 24 (HAstV4, MLB1, MLB2, VA1) hours post-infection, and total RNA was extracted, fragmented, depleted for rRNA and sequenced.
The purpose of the study was to investigate the dynamics of viral RNA production during astrovirus infection. Caco-2 cells were infected at MOI 5 with HAstV1, HAstV4 or VA1; Huh7.5.1 cells were infected at MOI 5 with MLB1 or MLB2. Cells were harvested at 6, 12, 18, 24 (HAstV1) or 24 (HAstV4, MLB1, MLB2, VA1) hours post-infection, and total RNA was extracted, fragmented, depleted for rRNA and sequenced.
European Bioinformatics Institute