Illumina amplicon sequencing of a degenerate 192 bp insert plasmid library for propagation profiling in E. coli
This dataset contains raw Illumina amplicon sequencing reads from a pooled competitive growth assay of a plasmid library carrying degenerate 192 bp inserts cloned into a standardised backbone. Degenerate oligonucleotide inserts were synthesised by Integrated DNA Technologies (IDT) and cloned into a plasmid backbone via Golden Gate assembly. The resulting library was transformed into E. coli DH5a. Sequencing was performed by Azenta at two timepoints (T0 and T16) across two biological replicates to quantify the relative abundance of sequence variants and calculate log2 fold change (LFC) as a measure of plasmid propagation fitness. After processing, 124,339 variants with sufficient coverage across both timepoints were retained for downstream analysis. This data is associated with the manuscript "Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation"
This dataset contains raw Illumina amplicon sequencing reads from a pooled competitive growth assay of a plasmid library carrying degenerate 192 bp inserts cloned into a standardised backbone. Degenerate oligonucleotide inserts were synthesised by Integrated DNA Technologies (IDT) and cloned into a plasmid backbone via Golden Gate assembly. The resulting library was transformed into E. coli DH5a. Sequencing was performed by Azenta at two timepoints (T0 and T16) across two biological replicates to quantify the relative abundance of sequence variants and calculate log2 fold change (LFC) as a measure of plasmid propagation fitness. After processing, 124,339 variants with sufficient coverage across both timepoints were retained for downstream analysis. This data is associated with the manuscript "Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation"
Thomas Copeman