RNA seq of human breast cancer cell line MDAMB231 with knockdown of splicing factors SRSF7 and SMNDC1
Deep RNA sequencing was performed to investigate the transcriptomic effects of siRNA-mediated knockdown of SRSF7 or SMNDC1 in the triple-negative breast cancer cell line MDA-MB-231. A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Stranded PolyA-selected libraries were prepared and sequenced on the DNBseq platform, generating 150-bp paired-end reads on a DNBSEQ-G400 sequencer (BGI Europe).
Deep RNA sequencing was performed to investigate the transcriptomic effects of siRNA-mediated knockdown of SRSF7 or SMNDC1 in the triple-negative breast cancer cell line MDA-MB-231. A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Stranded PolyA-selected libraries were prepared and sequenced on the DNBseq platform, generating 150-bp paired-end reads on a DNBSEQ-G400 sequencer (BGI Europe).
European Bioinformatics Institute