Analysis of gene expression in Porphyromonas gingivalis hemH deletion mutant strain grown under heme-depleted conditions (RNA-seq analysis)
Porphyromonas gingivalis, the keystone pathogen responsible for dysbiosis in the oral microbiome, the development of periodontal diseases, and the contribution to systemic comorbidities, is a heme auxotroph. It encodes only four enzymes in the heme biosynthesis pathway, namely HemD, HemN, HemG, and HemH. HemH protein is a ferrochelatase involved in the final step of heme biosynthesis by inserting iron ions (Fe2+) into protoporphyrin IX. This study aimed to analyze the effect of hemH gene deletion on P. gingivalis gene expression, thereby verifying its importance for P. gingivalis.
Porphyromonas gingivalis, the keystone pathogen responsible for dysbiosis in the oral microbiome, the development of periodontal diseases, and the contribution to systemic comorbidities, is a heme auxotroph. It encodes only four enzymes in the heme biosynthesis pathway, namely HemD, HemN, HemG, and HemH. HemH protein is a ferrochelatase involved in the final step of heme biosynthesis by inserting iron ions (Fe2+) into protoporphyrin IX. This study aimed to analyze the effect of hemH gene deletion on P. gingivalis gene expression, thereby verifying its importance for P. gingivalis.
European Bioinformatics Institute