RNA-seq of induced mitochronrial import stress and neurodegeneration in Drosophila
We adapted the yeast clogger system to Drosophila motoneurons to block TOM-TIM23-mediated import with temporal control. Sustained import stress converts somatic mitochondria into donut-shaped structures, depletes functional mitochondria from synaptic terminals, and causes progressive neurodegeneration with impaired neurotransmitter release and locomotor dysfunction. This neurodegeneration is mechanistically distinct from mitochondrial absence, as miro mutant neurons that completely lack presynaptic mitochondria do not degenerate. This dataset includes RNA-seq reads from an experiment when mito-IMMDHFR expression was induced pan-neuronally using the TARGET system (n-syb-Gal4; tub-Gal80ts) by shifting animals from 18?C to 29?C for 1, 2, or 3 days prior to collection at the wandering third instar larval stage.
We adapted the yeast clogger system to Drosophila motoneurons to block TOM-TIM23-mediated import with temporal control. Sustained import stress converts somatic mitochondria into donut-shaped structures, depletes functional mitochondria from synaptic terminals, and causes progressive neurodegeneration with impaired neurotransmitter release and locomotor dysfunction. This neurodegeneration is mechanistically distinct from mitochondrial absence, as miro mutant neurons that completely lack presynaptic mitochondria do not degenerate. This dataset includes RNA-seq reads from an experiment when mito-IMMDHFR expression was induced pan-neuronally using the TARGET system (n-syb-Gal4; tub-Gal80ts) by shifting animals from 18?C to 29?C for 1, 2, or 3 days prior to collection at the wandering third instar larval stage.
European Bioinformatics Institute