Different approaches to determine the composition and structure of the gut microbiota en Blattella germanica
A total of 50 samples were analysed by amplification of the V3-V4 region of the bacterial 16S rRNA gene, and the resulting amplicons were sequenced with a 2 × 300 bp paired-end reads on a MiSeq platform (Illumina).  In order to gain understanding of the influence of the environment, feces, and the genetic background of the host species on the acquisition and development of the gut microbiota in adult cockroaches, four essays were performed using 16S rRNA gene amplicon sequencing to analyze the hindgut and feces. We investigated the effect of the environment on two twinned Blattella germanica lab populations separated for two decades. We also studied the effect of the environment in one generation in two germ-free B. germanica populations reared in different laboratories. Besides, we compared the fecal microbiota of B. germanica, Periplaneta. americana and Blatta orientalis, to choose the most suitable donor for subsequent inter-species fecal transplant.
A total of 50 samples were analysed by amplification of the V3-V4 region of the bacterial 16S rRNA gene, and the resulting amplicons were sequenced with a 2 × 300 bp paired-end reads on a MiSeq platform (Illumina).  In order to gain understanding of the influence of the environment, feces, and the genetic background of the host species on the acquisition and development of the gut microbiota in adult cockroaches, four essays were performed using 16S rRNA gene amplicon sequencing to analyze the hindgut and feces. We investigated the effect of the environment on two twinned Blattella germanica lab populations separated for two decades. We also studied the effect of the environment in one generation in two germ-free B. germanica populations reared in different laboratories. Besides, we compared the fecal microbiota of B. germanica, Periplaneta. americana and Blatta orientalis, to choose the most suitable donor for subsequent inter-species fecal transplant.
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