Microbiota of feces and cecum from pigs fed with different source of oil
The growing demand for animal products with improved nutritional profiles and reduced saturated fat content has increased interest in dietary strategies that modulate the gut microbiota in swine. This study evaluated the impact of different lipid sources on the cecal and fecal bacterial communities of pigs. Ninety-six immunocastrated males were assigned to four treatments in a randomized complete block design. They fed a basal corn–soybean meal diet supplemented with 3% canola oil, 3% fish oil, 3% soybean oil, or 1.5% soybean oil. After a 98-day feeding period, cecal (n = 61) and fecal (n = 71) samples were collected at slaughter. Bacterial genomic DNA was extracted, and the V3–V4 region of the 16S rRNA gene was sequenced using the Illumina NextSeq 1000 platform. Sequence processing, ASV inference, and taxonomic classification were performed using DADA2 and phyloseq.
The growing demand for animal products with improved nutritional profiles and reduced saturated fat content has increased interest in dietary strategies that modulate the gut microbiota in swine. This study evaluated the impact of different lipid sources on the cecal and fecal bacterial communities of pigs. Ninety-six immunocastrated males were assigned to four treatments in a randomized complete block design. They fed a basal corn–soybean meal diet supplemented with 3% canola oil, 3% fish oil, 3% soybean oil, or 1.5% soybean oil. After a 98-day feeding period, cecal (n = 61) and fecal (n = 71) samples were collected at slaughter. Bacterial genomic DNA was extracted, and the V3–V4 region of the 16S rRNA gene was sequenced using the Illumina NextSeq 1000 platform. Sequence processing, ASV inference, and taxonomic classification were performed using DADA2 and phyloseq.
University of Sao Paulo