Stage-specific analysis of L. donovani transcriptome
Leishmania parasites cycle between the sand fly as promastigotes and the mammalian host cells as amastigotes. Throughout their life cycle the parasites respond to external changes such as pH and temperature shifts or starvation that trigger differentiation from one stage to another. Such processes rely on several regulatory mechanisms occurring at different levels of gene expression including post-transcriptional and translational level. We applied high throughput RNA sequencing  on L. donovani amastigotes purified from the spleen of infected hamsters and the freshly derived promastigotes (after 2 passages in vitro) to investigate their respective transcriptomic signature. RNA was prepared from four biological replicates for each condition.
Leishmania parasites cycle between the sand fly as promastigotes and the mammalian host cells as amastigotes. Throughout their life cycle the parasites respond to external changes such as pH and temperature shifts or starvation that trigger differentiation from one stage to another. Such processes rely on several regulatory mechanisms occurring at different levels of gene expression including post-transcriptional and translational level. We applied high throughput RNA sequencing  on L. donovani amastigotes purified from the spleen of infected hamsters and the freshly derived promastigotes (after 2 passages in vitro) to investigate their respective transcriptomic signature. RNA was prepared from four biological replicates for each condition.
European Bioinformatics Institute