mRNA-Seq of R. subcapitata exposed to different concentrations of the zinc oxide nanoparticle NM-110 against untreated control groups
n the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the zinc oxide nanoparticle NM-110. R. subcapitata was exposed to the ZnO NP NM-110 according to OECD guidelines (OECD test No. 201). At the end of exposure time (72 hours), simultaneous RNA and protein extraction was performed using a newly established RNA extraction protocol combinded with the Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Novaseq6000 system with a read length of 150 base pairs in paired-end run mode and the obtained sequences went through bioinformatic analysis pipeline to identify and count the detected gene sequences followed by differential gene expression analysis and overrepresentation analysis
n the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the zinc oxide nanoparticle NM-110. R. subcapitata was exposed to the ZnO NP NM-110 according to OECD guidelines (OECD test No. 201). At the end of exposure time (72 hours), simultaneous RNA and protein extraction was performed using a newly established RNA extraction protocol combinded with the Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Novaseq6000 system with a read length of 150 base pairs in paired-end run mode and the obtained sequences went through bioinformatic analysis pipeline to identify and count the detected gene sequences followed by differential gene expression analysis and overrepresentation analysis
European Bioinformatics Institute