mRNA-Seq of R. subcapitata exposed to different concentrations of the herbicide bentazone against untreated control groups
In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the herbicide bentazone (CAS RN 25057-89-0). R. subcapitata was exposed to bentazone according to OECD guidelines (OECD test No. 201). At the end of exposure time (72 hours), simultaneous RNA and protein extraction was performed using a newly established RNA extraction protocol combinded with the Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Novaseq6000 system with a read length of 150 base pairs in paired-end run mode and the obtained sequences went through bioinformatic analysis pipeline to identify and count the detected gene sequences followed by differential gene expression analysis and overrepresentation analysis
In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the herbicide bentazone (CAS RN 25057-89-0). R. subcapitata was exposed to bentazone according to OECD guidelines (OECD test No. 201). At the end of exposure time (72 hours), simultaneous RNA and protein extraction was performed using a newly established RNA extraction protocol combinded with the Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Novaseq6000 system with a read length of 150 base pairs in paired-end run mode and the obtained sequences went through bioinformatic analysis pipeline to identify and count the detected gene sequences followed by differential gene expression analysis and overrepresentation analysis
European Bioinformatics Institute