RNA-seq and ChIP-seq data in ring1ab, bmi1abc and compensating lines
Polycomb group (PcG) proteins play a crucial role in the epigenetic regulation of eukaryotes development. Two main complexes, Polycomb Repressive Complex 1 and 2 (PRC1/2), are responsible for the ubiquitination of histone H2A (H2Aub) and the methylation of histone H3 at lysine 27 (H3K27me3), respectively. While the core complex composition and function of PRC2 are conserved across different species and well-studied, the subunits of PRC1 are diverse and still not fully understood. In this study, we verified the conserved enzymatic activity of B-cell-specific Moloney murine leukaemia virus integration site 1 (BMI1A/B/C) in the green lineage, whose homolog proteins in animals do not have catalytic activity but assist the Really Interesting New Gene 1 (RING1) proteins for H2A monoubiquitination. We found that the genes regulated by both RING1s and BMI1s tend to have higher levels of H2Aub. Only-H2Aub genes are linked to high expression and H3K4me3 modification, and the evident repression of PcG protein is led by H3K27me3. Mutating the catalytic centers of RING-finger proteins severely affects the function of RING-finger domain proteins, as the RING-finger proteins can recruit H3K27me3 alone at specific regions, although H2Aub accomplishes the major recruitment.
Polycomb group (PcG) proteins play a crucial role in the epigenetic regulation of eukaryotes development. Two main complexes, Polycomb Repressive Complex 1 and 2 (PRC1/2), are responsible for the ubiquitination of histone H2A (H2Aub) and the methylation of histone H3 at lysine 27 (H3K27me3), respectively. While the core complex composition and function of PRC2 are conserved across different species and well-studied, the subunits of PRC1 are diverse and still not fully understood. In this study, we verified the conserved enzymatic activity of B-cell-specific Moloney murine leukaemia virus integration site 1 (BMI1A/B/C) in the green lineage, whose homolog proteins in animals do not have catalytic activity but assist the Really Interesting New Gene 1 (RING1) proteins for H2A monoubiquitination. We found that the genes regulated by both RING1s and BMI1s tend to have higher levels of H2Aub. Only-H2Aub genes are linked to high expression and H3K4me3 modification, and the evident repression of PcG protein is led by H3K27me3. Mutating the catalytic centers of RING-finger proteins severely affects the function of RING-finger domain proteins, as the RING-finger proteins can recruit H3K27me3 alone at specific regions, although H2Aub accomplishes the major recruitment.
PEKING UNIVERSITY