RNA-Seq on mouse murine duodenal intestinal organoids/tumoroids exposed to conditioned media from duodenal intestinal smooth muscle explants
This experiment sought to explore the niche factors that intestinal smooth muscle tissue provide to intestinal epithelium, and how this can affect epithelial growth. We first generated a conditioned medium from dissected duodenal smooth muscle explants, then used this conditioned medium to treat 3 intestinal organoid lines from WT C57Bl6 mice and 3 intestinal tumoroid lines from ApcMin mice (C57Bl6 background) 2 days after seeding. To see initial gene expression changes and how these develop over time, we collected samples for bulk RNASeq at 0min (untreated control), 20min, 2h, and 24h after treatment with conditioned medium.
This experiment sought to explore the niche factors that intestinal smooth muscle tissue provide to intestinal epithelium, and how this can affect epithelial growth. We first generated a conditioned medium from dissected duodenal smooth muscle explants, then used this conditioned medium to treat 3 intestinal organoid lines from WT C57Bl6 mice and 3 intestinal tumoroid lines from ApcMin mice (C57Bl6 background) 2 days after seeding. To see initial gene expression changes and how these develop over time, we collected samples for bulk RNASeq at 0min (untreated control), 20min, 2h, and 24h after treatment with conditioned medium.
European Bioinformatics Institute