Small RNA-sequencing of Indian OSCC Saliva samples
Saliva is a valuable liquid biopsy tool for the early detection of cancer. Especially in the case of oral cancer, it is in close proximity with the tumor site and can act as a valuable resource for identifying the diagnostic molecules that are associated with the tumor progression. miRNAs are the small non-coding RNAs that are shown to have excellent biomarker potential due to their disease-specific expression and the presence in multiple extra-cellular fluids. Small-RNA sequencing is one of the widely utilized methods for the detection of miRNAs. However, it is quite challenging for sequencing the clinical samples derived from the extra-cellular fluids such as saliva due to the low-yield. An ideal biomarker candidate should not only segregate between the classes but also should have good expression in the patient samples. To identify the presence of salivary miRNAs in the OSCC samples, small RNA-sequencing was performed using Qiaseq miRNA protocol.
Saliva is a valuable liquid biopsy tool for the early detection of cancer. Especially in the case of oral cancer, it is in close proximity with the tumor site and can act as a valuable resource for identifying the diagnostic molecules that are associated with the tumor progression. miRNAs are the small non-coding RNAs that are shown to have excellent biomarker potential due to their disease-specific expression and the presence in multiple extra-cellular fluids. Small-RNA sequencing is one of the widely utilized methods for the detection of miRNAs. However, it is quite challenging for sequencing the clinical samples derived from the extra-cellular fluids such as saliva due to the low-yield. An ideal biomarker candidate should not only segregate between the classes but also should have good expression in the patient samples. To identify the presence of salivary miRNAs in the OSCC samples, small RNA-sequencing was performed using Qiaseq miRNA protocol.
European Bioinformatics Institute