Gut microbiome of Tenebrio molitor after mycotoxin consumption
This study investigates the gut microbiota of Tenebrio molitor larvae exposed to the mycotoxins Aflatoxin B1, Fumonisin B1, and Deoxynivalenol. Larvae were reared on a wheat-bran diet supplemented with these toxins. Total DNA was extracted from larval intestines using the DNeasy PowerFood Microbial Kit. The V3-V4 region of the 16S rRNA gene was amplified using Bakt_341F/Bakt_805R primers (CCTACGGGNGGCWGCAG / GACTACHVGGGTATCTAATCC). The samples were sequenced using a paired-end, 2x300pb cycle run on an Illumina MiSeq sequencing system. Primers were not trimmed before deposition. The original study integrated both traditional culturing and metataxonomics to evaluate the impact of mycotoxins on larval development, survival, and gut microbiota
This study investigates the gut microbiota of Tenebrio molitor larvae exposed to the mycotoxins Aflatoxin B1, Fumonisin B1, and Deoxynivalenol. Larvae were reared on a wheat-bran diet supplemented with these toxins. Total DNA was extracted from larval intestines using the DNeasy PowerFood Microbial Kit. The V3-V4 region of the 16S rRNA gene was amplified using Bakt_341F/Bakt_805R primers (CCTACGGGNGGCWGCAG / GACTACHVGGGTATCTAATCC). The samples were sequenced using a paired-end, 2x300pb cycle run on an Illumina MiSeq sequencing system. Primers were not trimmed before deposition. The original study integrated both traditional culturing and metataxonomics to evaluate the impact of mycotoxins on larval development, survival, and gut microbiota
Complutense University of Madrid