RNA-seq of mouse CD8+ T cells treated with 25-HC versus DMSO control
To investigate the mechanism by which 25-HC restricts cytotoxic T cell differentiation, we performed RNA-seq on 25-HC-treated CD8+ T cells.Total RNA was extracted from activated CD8+ T cells, treated with 250 nM 25-HC or DMSO, using TRIzol reagent.
To investigate the mechanism by which 25-HC restricts cytotoxic T cell differentiation, we performed RNA-seq on 25-HC-treated CD8+ T cells.Total RNA was extracted from activated CD8+ T cells, treated with 250 nM 25-HC or DMSO, using TRIzol reagent.
European Bioinformatics Institute