RNA-seq of gastrocnemius muscle from control untreated BALB/c mice, cachectic C26 tumour-bearing mice treated with empty AAV, and cachectic C26 tumour-bearing mice treated with AAV encoding Melusin.
The aim of this experiment was to investigate the molecular changes that occur in skeletal muscle when the expression of the chaperone protein Melusin is maintained during cancer cachexia. To achieve this, BALB/c mice were intramuscularly injected with an AAV9 vector encoding a human Melusin construct, allowing for exogenous expression of Melusin in skeletal muscle. Fourteen days later, to induce cancer cachexia, mice received a subcutaneous injection of C26 colon carcinoma cells and were sacrificed twelve days after tumor implantation. Control tumour-bearing mice were injected with an empty AAV9 vector. RNA-seq was performed on gastrocnemius muscle tissue to assess transcriptional changes associated with Melusin expression under cachectic conditions
The aim of this experiment was to investigate the molecular changes that occur in skeletal muscle when the expression of the chaperone protein Melusin is maintained during cancer cachexia. To achieve this, BALB/c mice were intramuscularly injected with an AAV9 vector encoding a human Melusin construct, allowing for exogenous expression of Melusin in skeletal muscle. Fourteen days later, to induce cancer cachexia, mice received a subcutaneous injection of C26 colon carcinoma cells and were sacrificed twelve days after tumor implantation. Control tumour-bearing mice were injected with an empty AAV9 vector. RNA-seq was performed on gastrocnemius muscle tissue to assess transcriptional changes associated with Melusin expression under cachectic conditions
European Bioinformatics Institute