Ribosome-associated tRFs in yeast
Ribosome-associated noncoding RNAs (rancRNAs) are emerging regulators of translation, yet their composition and stress-dependent dynamics remain insufficiently understood. This study introduces a high-resolution method combining polysome profiling with AlkB-assisted small RNA sequencing to analyze rancRNAs across ribosomal fractions in Saccharomyces cerevisiae under sugar starvation and osmotic stress. AlkB treatment markedly enhances tRF detection, especially in subunit and monosome fractions. Overall, the dat reveals the dynamic, stress-responsive nature of tDRs as ribosome-associated regulatory molecules and provides a robust platform for rancRNA discovery.
Ribosome-associated noncoding RNAs (rancRNAs) are emerging regulators of translation, yet their composition and stress-dependent dynamics remain insufficiently understood. This study introduces a high-resolution method combining polysome profiling with AlkB-assisted small RNA sequencing to analyze rancRNAs across ribosomal fractions in Saccharomyces cerevisiae under sugar starvation and osmotic stress. AlkB treatment markedly enhances tRF detection, especially in subunit and monosome fractions. Overall, the dat reveals the dynamic, stress-responsive nature of tDRs as ribosome-associated regulatory molecules and provides a robust platform for rancRNA discovery.
ADAM MICKIEWICZ UNIVERSITY, POZNAN, POLAND