RNA-seq profiling of BMSCs with Nsun2 knockdown
This project aims to investigate the role of the RNA methyltransferase Nsun2 in the transcriptome regulation of bone marrow mesenchymal stem cells (BMSCs). We performed high-throughput RNA sequencing (RNA-seq) to compare the global gene expression profiles between control BMSCs and BMSCs in which Nsun2 was knocked down by siRNA. The objective of this study is to identify differentially expressed genes and altered biological pathways resulting from Nsun2 depletion, which will shed light on the molecular functions of Nsun2 in BMSC self-renewal, differentiation, or other cellular processes. Our data provides a valuable resource for understanding the epigenetic regulation of stem cells by RNA methylation.
BioProject