Transcriptomic responses of the porcine oviduct epithelial cells to cortisol levels for a prolonged period in a 3D air-liquid-interface culture system
An elevated cortisol level has been associated with negative impacts including animal reproduction. However, underlying molecular mechanisms and pathways are yet to be interrogated. In this experiment, we treated air-liquid interface cultures of the porcine oviduct epithelium derived from individual pigs (n=6) with 0, 100 and 250 nM cortisol for a prolonged culture period of 21 days. Cells were harvested for RNA-seq analysis at 3-hour, 12-hour, 3-day and 21-day post treatments.
An elevated cortisol level has been associated with negative impacts including animal reproduction. However, underlying molecular mechanisms and pathways are yet to be interrogated. In this experiment, we treated air-liquid interface cultures of the porcine oviduct epithelium derived from individual pigs (n=6) with 0, 100 and 250 nM cortisol for a prolonged culture period of 21 days. Cells were harvested for RNA-seq analysis at 3-hour, 12-hour, 3-day and 21-day post treatments.
European Bioinformatics Institute