Transcriptomic effects of 33 PFAS in HepaRG cells
Per- and polyfluoroalkyl substances (PFAS) are a large family of more than 10,000 different congeners that are used in the fabrication of numerous industry and consumer products. Due to their high persistence and adverse health effects in humans, the use of a number of PFAS has been restricted. Thus, novel PFAS are increasingly used for their industrial applications, despite the fact that currently limited or no data is available on their toxicity. This study aims to examine the molecular mechanisms of action of novel PFAS with a focus on the subgroup of mono- and polyether PFAS, with both linear and branched structures, including PFAS congeners with either a carboxylic acid or a sulfonic acid functional group. Differentiated HepaRG cells, a model for human hepatocytes, were incubated for 24 h with 33 different PFAS congeners at three non-cytotoxic concentrations, and transcriptomic analysis was subsequently performed.
Per- and polyfluoroalkyl substances (PFAS) are a large family of more than 10,000 different congeners that are used in the fabrication of numerous industry and consumer products. Due to their high persistence and adverse health effects in humans, the use of a number of PFAS has been restricted. Thus, novel PFAS are increasingly used for their industrial applications, despite the fact that currently limited or no data is available on their toxicity. This study aims to examine the molecular mechanisms of action of novel PFAS with a focus on the subgroup of mono- and polyether PFAS, with both linear and branched structures, including PFAS congeners with either a carboxylic acid or a sulfonic acid functional group. Differentiated HepaRG cells, a model for human hepatocytes, were incubated for 24 h with 33 different PFAS congeners at three non-cytotoxic concentrations, and transcriptomic analysis was subsequently performed.
polo d'innovazione di genomica, genetica e biologia