Large population sizes minimize growth and collateral sensitivity trade-offs during antibiotic resistance development
Replicate Staphylococcus aureus Newman lineages were evolved under a small (10^9) and large (10^11) population for three antibiotics (clindamycin, levofloxacin and oxacillin). The antibiotic concentration was increased daily by a factor 2^(1/3) starting from 0.25x MIC. After 160 generations, DNA was extracted from endpoint populations and submitted to high-throughput sequencing.
Replicate Staphylococcus aureus Newman lineages were evolved under a small (10^9) and large (10^11) population for three antibiotics (clindamycin, levofloxacin and oxacillin). The antibiotic concentration was increased daily by a factor 2^(1/3) starting from 0.25x MIC. After 160 generations, DNA was extracted from endpoint populations and submitted to high-throughput sequencing.
Centre of Microbial and Plant Genetics