Concatenated COI gene of Tephritidae from Uganda
A total of 39 Tephritidae samples were collected from 20 mango orchards across seven districts in two mango-growing agro-ecological zones of Uganda between May and August 2023. Insects were trapped using Methyl eugenol, Cue Lure, Enrich Ginger Oil, and Brewer's yeast. DNA was extracted using a modified Zymo Quick-DNA protocol, and PCR amplification was performed with the primers CI-J2183 (5'-CAACATTTATTTTGATTTTTTGG-3') and TL2-N3014 (5'-TCCAATGCACTAATCTGCCATATT-3'). PCR products were double-read by Sanger sequencing at Inqaba Biotec. Sequence quality was assessed using Chromas, concatenated in MEGA 11, and identified through BLAST searches in NCBI.
A total of 39 Tephritidae samples were collected from 20 mango orchards across seven districts in two mango-growing agro-ecological zones of Uganda between May and August 2023. Insects were trapped using Methyl eugenol, Cue Lure, Enrich Ginger Oil, and Brewer's yeast. DNA was extracted using a modified Zymo Quick-DNA protocol, and PCR amplification was performed with the primers CI-J2183 (5'-CAACATTTATTTTGATTTTTTGG-3') and TL2-N3014 (5'-TCCAATGCACTAATCTGCCATATT-3'). PCR products were double-read by Sanger sequencing at Inqaba Biotec. Sequence quality was assessed using Chromas, concatenated in MEGA 11, and identified through BLAST searches in NCBI.
ONEN DENISH OYARO