Role of KDM5C knock down on histone marks
To decipher the role of KDM5C knock down on histone marks, 786-O human renal cell carcinoma cell line was transduced stably with shKDM5C or with shKDM5C+ KDM5C ORF to rescue the loss of KDM5C. Cells were injected s.c in mice and tumors reaching 100mm3 were treated with sunitinib (SU-11248, Pfizer, Inc., New Yourk) at dose of 50mg/kg/day SID. Recovered tumors were snap frozen in liquid nitrogen and were used for ChIP-seq using antibodies against H3, H3K4me3, H3K4me1 and H3K27ac.
To decipher the role of KDM5C knock down on histone marks, 786-O human renal cell carcinoma cell line was transduced stably with shKDM5C or with shKDM5C+ KDM5C ORF to rescue the loss of KDM5C. Cells were injected s.c in mice and tumors reaching 100mm3 were treated with sunitinib (SU-11248, Pfizer, Inc., New Yourk) at dose of 50mg/kg/day SID. Recovered tumors were snap frozen in liquid nitrogen and were used for ChIP-seq using antibodies against H3, H3K4me3, H3K4me1 and H3K27ac.
European Bioinformatics Institute