In this study we studied transcriptomaic changes in U87 glioma cell line after knockdown of circATXN and lin ATXN transcripts with siRNA.
We identify circATXN10 as a previously uncharacterized circRNA significantly downregulated in GBM. CircATXN10 originates from the ATXN10 locus and exhibits a reciprocal regulatory relationship with its linear counterpart, linATXN10. Modulation of either isoform inversely affected the expression of the other, revealing a bidirectional regulatory mechanism involving competitive splicing, RNA–RNA interactions, and post-transcriptional control of transcript stability.
We identify circATXN10 as a previously uncharacterized circRNA significantly downregulated in GBM. CircATXN10 originates from the ATXN10 locus and exhibits a reciprocal regulatory relationship with its linear counterpart, linATXN10. Modulation of either isoform inversely affected the expression of the other, revealing a bidirectional regulatory mechanism involving competitive splicing, RNA–RNA interactions, and post-transcriptional control of transcript stability.
Institute of Bioorganic Chemistry. Polish Acedemy of Sciences