Single cell sequencing of mucinous colorectal carcinoma
This project aims to systematically characterize the intratumoral heterogeneity and immune microenvironment of MAC using single-cell RNA sequencing (scRNA-seq). Tumor tissues and matched adjacent normal mucosa were collected from patients pathologically diagnosed with mucinous colorectal carcinoma. Single-cell suspensions were prepared following tissue dissociation, red blood cell lysis, and viability assessment. Libraries were constructed using the 10x Genomics Chromium platform.
This project aims to systematically characterize the intratumoral heterogeneity and immune microenvironment of MAC using single-cell RNA sequencing (scRNA-seq). Tumor tissues and matched adjacent normal mucosa were collected from patients pathologically diagnosed with mucinous colorectal carcinoma. Single-cell suspensions were prepared following tissue dissociation, red blood cell lysis, and viability assessment. Libraries were constructed using the 10x Genomics Chromium platform.
The Second Affiliated Hospital of Nanchang University