Transcriptomic responses of zebrafish embryos to sublethal exposure to perfluorooctanoic acid (PFOA): A 96-Hour RNA-seq Study
Per- and polyfluoroalkyl substances (PFAS) are persistent synthetic chemicals widely detected in the environment and associated with adverse effects such as developmental toxicity, endocrine disruption, and hepatotoxicity. Traditional chemical risk assessment relies on chronic animal studies, which are resource- and time-intensive and provide limited mechanistic insight. In this study, zebrafish embryos were exposed to sublethal concentrations of PFOA over 96 hours, and RNA sequencing was performed to assess concentration-dependent changes in gene expression. The dataset enables identification of sensitive transcriptional responses and affected biological pathways, supporting the use of transcriptomic approaches for mechanistic and ethically compliant hazard assessment of PFAS.
Per- and polyfluoroalkyl substances (PFAS) are persistent synthetic chemicals widely detected in the environment and associated with adverse effects such as developmental toxicity, endocrine disruption, and hepatotoxicity. Traditional chemical risk assessment relies on chronic animal studies, which are resource- and time-intensive and provide limited mechanistic insight. In this study, zebrafish embryos were exposed to sublethal concentrations of PFOA over 96 hours, and RNA sequencing was performed to assess concentration-dependent changes in gene expression. The dataset enables identification of sensitive transcriptional responses and affected biological pathways, supporting the use of transcriptomic approaches for mechanistic and ethically compliant hazard assessment of PFAS.
European Bioinformatics Institute