Acartia tonsa microbiome
16S sequencing samples from Acartia tonsa eggs (provided from CFEED.no, stored at 5°C) and rhodomonas baltica (provided from CFEED.no, around 15 million algae cells/mL). Eggs were incubated 48h at 20°C in 4 treatments: control, prebiotic (scFOS, 5 mg/L), probiotic (bacillus, 100 mg/L) and synbiotic (scFOS 5 mg/L, bacillus 100 mg/L). Seawater used in hatching was collected as well, and the commercial probiotic mix (MIC-F, inve). Nauplii were sieved off (40 µm) and collected for 16S sequencing of microbial community. Nauplii from prebiotic treatment: BM_11/17/23. Nauplii control: BM_12/18/24. Nauplii probiotic treatment: BM_13/19/25. Nauplii from synbiotic treatment: BM_14/20/26.
16S sequencing samples from Acartia tonsa eggs (provided from CFEED.no, stored at 5°C) and rhodomonas baltica (provided from CFEED.no, around 15 million algae cells/mL). Eggs were incubated 48h at 20°C in 4 treatments: control, prebiotic (scFOS, 5 mg/L), probiotic (bacillus, 100 mg/L) and synbiotic (scFOS 5 mg/L, bacillus 100 mg/L). Seawater used in hatching was collected as well, and the commercial probiotic mix (MIC-F, inve). Nauplii were sieved off (40 µm) and collected for 16S sequencing of microbial community. Nauplii from prebiotic treatment: BM_11/17/23. Nauplii control: BM_12/18/24. Nauplii probiotic treatment: BM_13/19/25. Nauplii from synbiotic treatment: BM_14/20/26.
University of Ghent