The timescale of nematode evolution reveals late and convergent radiations of parasitism
Transcriptome sequencing was performed on RNA samples from 5 nematode species. Samples of parasitic Brumptaemilius sp. (Rhigonematomorpha: Carnoyidae) and Thelastoma sp. (Oxyuridomorpha: Thelastomatidae) were isolated from the intestinal tract of a single millipede (Archispirostreptus gigas). Samples of three free-living species, Chromadoropsis vivipara, Enoplus sp., Cylicolaimus sp., were captured in Tjärnö Marine Laboratory (Sweden), ground and preserved in Qiagen RNAlater and stored at -80 °C until additional processing. Total RNA was extracted with TRIzol (Ambion, Austin, USA). Agilent Technologies Bioanalyzer Sensitivity RNA was used for quality control analysis and quantification of the total RNA, at the genomics facility at the University of Bristol. Poly(A) RNA sequencing library was prepared following NEBNext Ultra II Directional RNA-Seq library (poly-A selection, 200–300bp inserts) protocol and paired-ended sequencing was performed using NovaSeq 6000 SP v1.5 ow cell with 150bp PE reads, service provided by the Earlham Institute, Norwich, UK.
Transcriptome sequencing was performed on RNA samples from 5 nematode species. Samples of parasitic Brumptaemilius sp. (Rhigonematomorpha: Carnoyidae) and Thelastoma sp. (Oxyuridomorpha: Thelastomatidae) were isolated from the intestinal tract of a single millipede (Archispirostreptus gigas). Samples of three free-living species, Chromadoropsis vivipara, Enoplus sp., Cylicolaimus sp., were captured in Tjärnö Marine Laboratory (Sweden), ground and preserved in Qiagen RNAlater and stored at -80 °C until additional processing. Total RNA was extracted with TRIzol (Ambion, Austin, USA). Agilent Technologies Bioanalyzer Sensitivity RNA was used for quality control analysis and quantification of the total RNA, at the genomics facility at the University of Bristol. Poly(A) RNA sequencing library was prepared following NEBNext Ultra II Directional RNA-Seq library (poly-A selection, 200–300bp inserts) protocol and paired-ended sequencing was performed using NovaSeq 6000 SP v1.5 ow cell with 150bp PE reads, service provided by the Earlham Institute, Norwich, UK.
University of Bristol