Sludge_carbo_16S_bacteria_EPFL_2025
6S rRNA gene amplicon sequencing (Bacteria-specific primers) of anaerobic sludge microbial communities serially enriched on carbohydrate substrates (glucose, cellobiose, xylose, glucose+xylose, or no carbon source) across multiple pH conditions (5.5, 6.5, 7.5), four independent inocula, and up to eight transfer weeks. Sequencing was performed on the Element Biosciences AVITI platform (Cloudbreak FS Standard Output 600-cycle kit, paired-end 2x300bp). This study investigates environmental selection on metabolic network architecture despite taxonomic turnover in anaerobic digestion communities.
6S rRNA gene amplicon sequencing (Bacteria-specific primers) of anaerobic sludge microbial communities serially enriched on carbohydrate substrates (glucose, cellobiose, xylose, glucose+xylose, or no carbon source) across multiple pH conditions (5.5, 6.5, 7.5), four independent inocula, and up to eight transfer weeks. Sequencing was performed on the Element Biosciences AVITI platform (Cloudbreak FS Standard Output 600-cycle kit, paired-end 2x300bp). This study investigates environmental selection on metabolic network architecture despite taxonomic turnover in anaerobic digestion communities.
Ecole Polytechnique Federale de Lausanne