Elephant iPSC Direct RNA Sequencing
This study contains Oxford Nanopore Technologies direct RNA sequencing data generated from Asian elephant (Elephas maximus) induced pluripotent stem cells (emiPSCs) and related cell populations. The data were produced as part of a project establishing the first elephant induced pluripotent stem cell lines and were used to characterize transcriptome architecture, endogenous pluripotency gene expression, alternative splicing, poly(A) tail features, and RNA modifications.  Native RNA molecules were sequenced using the Oxford Nanopore SQK-RNA004 Direct RNA Sequencing Kit and basecalled with Dorado. Analyses included transcript isoform discovery, transcriptome characterization, and modified base detection. The dataset supports the study of elephant stem cell biology, cellular reprogramming, comparative transcriptomics, and conservation genomics.  Samples include elephant induced pluripotent stem cell lines generated through multiple reprogramming strategies and associated experimental replicates. These data contributed to the characterization of pluripotency and differentiation potential.
This study contains Oxford Nanopore Technologies direct RNA sequencing data generated from Asian elephant (Elephas maximus) induced pluripotent stem cells (emiPSCs) and related cell populations. The data were produced as part of a project establishing the first elephant induced pluripotent stem cell lines and were used to characterize transcriptome architecture, endogenous pluripotency gene expression, alternative splicing, poly(A) tail features, and RNA modifications.  Native RNA molecules were sequenced using the Oxford Nanopore SQK-RNA004 Direct RNA Sequencing Kit and basecalled with Dorado. Analyses included transcript isoform discovery, transcriptome characterization, and modified base detection. The dataset supports the study of elephant stem cell biology, cellular reprogramming, comparative transcriptomics, and conservation genomics.  Samples include elephant induced pluripotent stem cell lines generated through multiple reprogramming strategies and associated experimental replicates. These data contributed to the characterization of pluripotency and differentiation potential.
Weill Cornell Medicine Department of Physiology and Biophysics