Gut microbiota analysis in FMF, CAPS, and MKD
Introduction: Familial Mediterranean fever (FMF), cryopyrinopathies (CAPS) and mevalonate deficiency (MKD) are autoinflammatory diseases with gastrointestinal involvement. Dysbiosis has been suggested with 16S rRNA sequencing in FMF. Objective: to describe gut microbiota in FMF, CAPS, and MKD using shotgun sequencing.   Methods: 88 FMF, 13 CAPS, and 5 MKD adult patients underwent fecal shotgun metagenomic sequencing and metabolomic profiling.   Results: FMF patients exhibited gut dysbiosis: reduced diversity, depletion of SCFA-producing bacteria and bacteriophages. No dysbiosis was detected in CAPS or MKD.   Conclusion: Gut dysbiosis in FMF may contribute to inflammation. The absence of dysbiosis in CAPS and MKD suggests different mechanisms.
Introduction: Familial Mediterranean fever (FMF), cryopyrinopathies (CAPS) and mevalonate deficiency (MKD) are autoinflammatory diseases with gastrointestinal involvement. Dysbiosis has been suggested with 16S rRNA sequencing in FMF. Objective: to describe gut microbiota in FMF, CAPS, and MKD using shotgun sequencing.   Methods: 88 FMF, 13 CAPS, and 5 MKD adult patients underwent fecal shotgun metagenomic sequencing and metabolomic profiling.   Results: FMF patients exhibited gut dysbiosis: reduced diversity, depletion of SCFA-producing bacteria and bacteriophages. No dysbiosis was detected in CAPS or MKD.   Conclusion: Gut dysbiosis in FMF may contribute to inflammation. The absence of dysbiosis in CAPS and MKD suggests different mechanisms.
Centre de Recherche scientifique Saint-Antoine