single-cell RNA-Seq of human induced pluripotent stem cells from a healthy donor and a DMD patient at different time point during myogenic differentiation
The experiment aimed to validate the gene network regulating cell fate decisions during the myogenic differentiation of iPS cells from a DMD patient. DMD iPSCs were transfected with specific siRNA at Day 7 of the differentiation protocol and collected either at Day 9 or Day 14. As a control, we used a non-specific siRNA. Non transfected DMD cells were also included, together with the healthy control cell line used in our previous experiments.
The experiment aimed to validate the gene network regulating cell fate decisions during the myogenic differentiation of iPS cells from a DMD patient. DMD iPSCs were transfected with specific siRNA at Day 7 of the differentiation protocol and collected either at Day 9 or Day 14. As a control, we used a non-specific siRNA. Non transfected DMD cells were also included, together with the healthy control cell line used in our previous experiments.
European Bioinformatics Institute