Antimalarial drug resistance surveillance in Western Kenya using Nanopore amplicon sequencing
A total of 315 clinical isolates were collected from three sites  in Western Kenya (Homa Bay Mainland, Migori, and Mfangano Island)  between September 2020 and January 2024, of which 310 were analyzed.  DNA was extracted from dried blood spots and amplified for four drug  resistance genes: dhfr, dhps, mdr, and k13 along with Pfama1.  Amplicons were sequenced using Oxford Nanopore MinION technology with dual-barcode multiplexing (24 outer barcodes combined with 8 inner MID tags). This dataset supports surveillance of antimalarial resistance in  high-transmission areas.
A total of 315 clinical isolates were collected from three sites  in Western Kenya (Homa Bay Mainland, Migori, and Mfangano Island)  between September 2020 and January 2024, of which 310 were analyzed.  DNA was extracted from dried blood spots and amplified for four drug  resistance genes: dhfr, dhps, mdr, and k13 along with Pfama1.  Amplicons were sequenced using Oxford Nanopore MinION technology with dual-barcode multiplexing (24 outer barcodes combined with 8 inner MID tags). This dataset supports surveillance of antimalarial resistance in  high-transmission areas.
Strathmore Institute of Healthcare Management