Transcriptomic signatures of the porcine oviduct epithelial cells responding to an administration of cortisol and two selective glucocorticoid receptor (GR) antagonists; AL 082D06 and Cort108297 in an air-liquid-interface culture system
To unveil GR pathway involvement in the response of the oviduct epithelium to cortisol, we used two selective glucocorticoid receptor (GR) antagonists; AL 082D06 and Cort108297. In this experiment, we treated air-liquid interface cultures of the porcine oviduct epithelium, derived from individual pigs (n=6), from the basolateral side with the following conditions: 1) basal media control; 2) 250nM cortisol; 3) 250nM cortisol + 0.25µM AL 082D06; 4) 250nM cortisol + 2.5µM AL 082D06; 5) 250nM cortisol + 25µM AL 082D06; 6) 250nM cortisol + 0.025µM Cort108297; 7) 250nM cortisol + 0.25µM Cort108297; 8) 250nM cortisol + 2.5µM Cort108297. Cells were harvested for the RNA-seq analysis after 12 hours of the treatments.
To unveil GR pathway involvement in the response of the oviduct epithelium to cortisol, we used two selective glucocorticoid receptor (GR) antagonists; AL 082D06 and Cort108297. In this experiment, we treated air-liquid interface cultures of the porcine oviduct epithelium, derived from individual pigs (n=6), from the basolateral side with the following conditions: 1) basal media control; 2) 250nM cortisol; 3) 250nM cortisol + 0.25µM AL 082D06; 4) 250nM cortisol + 2.5µM AL 082D06; 5) 250nM cortisol + 25µM AL 082D06; 6) 250nM cortisol + 0.025µM Cort108297; 7) 250nM cortisol + 0.25µM Cort108297; 8) 250nM cortisol + 2.5µM Cort108297. Cells were harvested for the RNA-seq analysis after 12 hours of the treatments.
European Bioinformatics Institute