Functional analysis of ESB1 and co-functioning factors identified by compensatory transcriptome changes
Leishmania have an ortholog of ESB1, originally identified as controlling surface antigen expression in Trypanosoma brucei. Leishmania ESB1 can be deleted, with an initial strong growth defect which recovers over time. This study analyses the effect on the transcriptome of ESB1 deletion, how the transcriptome changes on growth rate recovery, and whether genomic changes happen causing the transcriptome change. This identified co-functioning factors, which were subsequently analysed by detecting transcriptomic change on their deletion.
Leishmania have an ortholog of ESB1, originally identified as controlling surface antigen expression in Trypanosoma brucei. Leishmania ESB1 can be deleted, with an initial strong growth defect which recovers over time. This study analyses the effect on the transcriptome of ESB1 deletion, how the transcriptome changes on growth rate recovery, and whether genomic changes happen causing the transcriptome change. This identified co-functioning factors, which were subsequently analysed by detecting transcriptomic change on their deletion.
University of Edinburgh