SMART-seq2 of Kdm5b Overexpression in Fully Grown Oocytes
RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 µg/µL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The oocytes were cultured overnight for approximately 16 h in M2 containing 20 µM Milrinone and 5% FBS and collected for sequencing.
RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 µg/µL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The oocytes were cultured overnight for approximately 16 h in M2 containing 20 µM Milrinone and 5% FBS and collected for sequencing.
European Bioinformatics Institute