Bulk RNA-sequencing of rat tibial cartilage during pre-osteoarthritis and early osteoarthritis progression
This dataset comprises bulk RNA sequencing of rat tibial articular cartilage across an early osteoarthritis (OA) progression time course, using the medial meniscectomy (MMx) surgical model.  Female 8-week-old Sprague-Dawley rats underwent medial meniscectomy of the right knee, and tibial articular cartilage from the medial middle region was collected at multiple time points: intact controls (no surgery), day 3, week 1, week 3, and week 6 post-surgery (n=3 biological replicates per group, 15 samples total). Total RNA was prepared from each cartilage sample, and bulk RNA-seq libraries were sequenced on an Illumina NovaSeq platform in paired-end 150 bp mode.  The dataset enables transcriptomic profiling of pre-osteoarthritic and early-stage osteoarthritic cartilage and identification of dynamically regulated pathways during OA initiation and early progression.
Showa Medical School