Small RNA sequencing of CBCVd-infected and control Humulus lupulus cv. Herkules leaves
Small RNA libraries from Humulus lupulus cultivar Herkules plants with natural HLVd background. Half of the plants were additionally infected with CBCVd (Citrus Bark Cracking Viroid) in August/October 2021. Leaf samples were collected during the symptomatic phase in May and August 2023 from four biological pools per treatment group (CBCVd-infected and control). Libraries were prepared using the TruSeq Small RNA kit and sequenced on Illumina NextSeq 550 (75bp SE). This study investigates viroid-host interactions and small RNA regulation in hop, with a focus on CBCVd-responsive miRNA and siRNA populations against a persistent HLVd co-infection background.
Small RNA libraries from Humulus lupulus cultivar Herkules plants with natural HLVd background. Half of the plants were additionally infected with CBCVd (Citrus Bark Cracking Viroid) in August/October 2021. Leaf samples were collected during the symptomatic phase in May and August 2023 from four biological pools per treatment group (CBCVd-infected and control). Libraries were prepared using the TruSeq Small RNA kit and sequenced on Illumina NextSeq 550 (75bp SE). This study investigates viroid-host interactions and small RNA regulation in hop, with a focus on CBCVd-responsive miRNA and siRNA populations against a persistent HLVd co-infection background.
Hochschule Geisenheim University