Dasineura rozkovi de novo transcriptome assembly and raw data
The larch bud gall midge (Dasineura rozhkovi Mamaev &amp; Nikolskij 1983) is one of themain pests infesting larches. Its activity causes developmental defects and anoticeable decrease in the population growth in adult Siberian larches (Larix sibirica).Due to its complex life cycle and active presence in areas of human habitation, thisinsect is difficult to exterminate using common methods. D. rozhkovi has not beenstudied before with the use of sequencing technologies, which means there is noinformation on the mechanisms occurring in its body at the molecular level. Here, wepresent for the first time a de novo assembly and analysis of the D. rozhkovitranscriptome. The de novo transcriptome assembly makes for a practical approach tothe reconstruction of the full transcript set in organisms with no reference genome,allowing researchers to identify new genes and isoforms as well as to study geneexpression patterns. We found a pronounced sexual dimorphism in differential geneexpression in D. rozhkovi imagoes and performed a phylogenetic analysis usingearlier-published genome assemblies of the insects belonging to several large clades.The resulting phylogenetic tree includes D. rozhkovi and 8 other gall midge speciesand shows the closest relationship between D. rozhkovi and the Hessian fly Mayetioladestructor. We believe that the obtained results can be further used for developingmeasures to control this pest.
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