Transcriptomic architecture of the porcine liver responding to Aflatoxin B1 (AFB1) and medicinal herb dietary treatments
Aflatoxins are metabolites produced by the Aspergillus genus fungi and Aflatoxin B1 (AFB1) is the most potent hepatotoxin among them. They often contaminate the agricultural crops and hence enter the food chain including livestock feed production. We have proposed the utilization of biotransformative herbal extracts on instigating the hepatic health and hepatotoxic effects exerted by AFB1 in pigs. In this experiment, we investigated the effects of Aflatoxin B1 (AFB1) and medicinal herb dietary supplementations on the transcriptomic architecture of the liver. Commercial crossbred TN70 pigs (Norsvin Landrace x Large White) were on a standard basal diet for 7 days of adaptation prior to assigning to the dietary treatments (n = 44); 1) Aflatoxin B1+ DMSO (120 µg/kg BW) (n = 6), 2) Andrographis paniculata (30 mg/kg BW) (n = 10), 3) Silybum marianum (90 mg/kg BW) (n = 10), 4) Curcuma longa (90 mg/kg BW) (n =10), 5) DMSO (n = 4), 6) Control (n = 4). The supplementation of medicinal herbs was provided to the pigs at the age of 42 days, while AFB1 was administered from day 56 until the end of experiment, 70 days of age. The pigs were slaughtered and the liver samples were collected for the RNA-seq analysis.
Aflatoxins are metabolites produced by the Aspergillus genus fungi and Aflatoxin B1 (AFB1) is the most potent hepatotoxin among them. They often contaminate the agricultural crops and hence enter the food chain including livestock feed production. We have proposed the utilization of biotransformative herbal extracts on instigating the hepatic health and hepatotoxic effects exerted by AFB1 in pigs. In this experiment, we investigated the effects of Aflatoxin B1 (AFB1) and medicinal herb dietary supplementations on the transcriptomic architecture of the liver. Commercial crossbred TN70 pigs (Norsvin Landrace x Large White) were on a standard basal diet for 7 days of adaptation prior to assigning to the dietary treatments (n = 44); 1) Aflatoxin B1+ DMSO (120 µg/kg BW) (n = 6), 2) Andrographis paniculata (30 mg/kg BW) (n = 10), 3) Silybum marianum (90 mg/kg BW) (n = 10), 4) Curcuma longa (90 mg/kg BW) (n =10), 5) DMSO (n = 4), 6) Control (n = 4). The supplementation of medicinal herbs was provided to the pigs at the age of 42 days, while AFB1 was administered from day 56 until the end of experiment, 70 days of age. The pigs were slaughtered and the liver samples were collected for the RNA-seq analysis.
European Bioinformatics Institute