RNA-seq of JADE1/2/3 wildtype and PZP mutants in HEK293T cell
We generated full length V5-tagged WT JADE1, JADE2 and JADE3 constructs and the constructs lacking the PZP domains (JADE1_PZP, JADE2_PZP and JADE3_PZP) and expressed these constructs transiently in HEK293T cells. 24 hours after transfection the cells were harvest. Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer's instructions, including a DNase1 treatment. Afterwards we performed unbiased transcriptomic mRNA-seq analysis to analyze the importance of JADE1, JADE2 and JADE3 PZP mutants in transcriptional regulation.
We generated full length V5-tagged WT JADE1, JADE2 and JADE3 constructs and the constructs lacking the PZP domains (JADE1_PZP, JADE2_PZP and JADE3_PZP) and expressed these constructs transiently in HEK293T cells. 24 hours after transfection the cells were harvest. Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer's instructions, including a DNase1 treatment. Afterwards we performed unbiased transcriptomic mRNA-seq analysis to analyze the importance of JADE1, JADE2 and JADE3 PZP mutants in transcriptional regulation.
European Bioinformatics Institute