HE513 (COI)
Water was sampled on cruise HE513 with RV Heincke from 04.06.-10.06.20018 in the North Sea ('Entenschnabel'). Sampling was conducted at 24 stations in intervals of 6 hours: the first 12 at a fixed station, thereafter the engines were turned off to allow drifting with the current. Water was prefiltered on 150 µm and 20 µm filters and subsequently on 3 µm and 0.2 µm filters (filter cascade). We performed metabarcoding with primers targeting the cytochrome oxidase c subunit I region (COI) with Leray-XT primers and another primer pair trgeting 18S, aiming for marine metazoan species.
German Federation for Biological Data