RNA-seq Analysis of Cumulus Cells from Mice with Oocyte-Specific DHX36 Knockout
To investigate the role of DHX36 in oocyte-somatic cell interaction, oocyte-specific DHX36 knockout mice were generated. The mutation led to reduced granulosa cell adhesion and proliferation, thereby disrupting follicular development through impaired oocyte-cumulus cell (CC) communication. To explore the molecular alterations in CCs underlying this phenotype, RNA sequencing was performed. CCs were collected from 30-40 cumulus-oocyte complexes (COCs) per group from both wild-type (WT) and conditional knockout (CKO) mice and subjected to Smart-seq2 library preparation. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform.
To investigate the role of DHX36 in oocyte-somatic cell interaction, oocyte-specific DHX36 knockout mice were generated. The mutation led to reduced granulosa cell adhesion and proliferation, thereby disrupting follicular development through impaired oocyte-cumulus cell (CC) communication. To explore the molecular alterations in CCs underlying this phenotype, RNA sequencing was performed. CCs were collected from 30-40 cumulus-oocyte complexes (COCs) per group from both wild-type (WT) and conditional knockout (CKO) mice and subjected to Smart-seq2 library preparation. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform.
Life Sciences Institute, Zhejiang University