Genome of the nematicid strain Stenotrophomonas INTA L402-1 isolated from cadavers of Tenebrio molitor (Coleoptera: Tenebrionidae) larvae infected with the EPN Heterorhabditis bacteriophora
Strain INTA L402-1 was isolated from the hemolymph of T. molitor larvae infected with the entomopathogenic nematode H. bacteriophora strain SUP. The nematodes were maintained using standard insect-baiting techniques. Infection assays were conducted by applying 500 µL of a suspension containing 5000 infective juveniles/mL onto filter paper discs in Petri dishes, followed by the addition of ten larvae per plate. After incubation at 25 ± 2 °C, infected larvae were identified by their characteristic reddish coloration. Hemolymph was extracted aseptically, and bacteria were isolated on NBTA agar, followed by purification on nutrient agar. Whole-genome sequencing, total DNA of INTA L402-1 strain was extracted using the Wizard Genomic DNA Purification Kit (Promega). The genome of the strain was sequenced using Illumina technology provided by the Unidad Operativa Centro Nacional de Genómica y Bioinformática (ANLIS Malbrán, Argentina). The genome assembly was performed de novo using SPAdes v3.15.4 genome assembler, and the resulting assembly underwent rigorous quality control assessment with QUAST v4.4 and CheckM v1.0.18 using KBase open-source software. Strain INTA L402-1 represents a putative novel species within the genus Stenotrophomonas based on genomic analyses, including average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and phylogenomic reconstruction.
Strain INTA L402-1 was isolated from the hemolymph of T. molitor larvae infected with the entomopathogenic nematode H. bacteriophora strain SUP. The nematodes were maintained using standard insect-baiting techniques. Infection assays were conducted by applying 500 µL of a suspension containing 5000 infective juveniles/mL onto filter paper discs in Petri dishes, followed by the addition of ten larvae per plate. After incubation at 25 ± 2 °C, infected larvae were identified by their characteristic reddish coloration. Hemolymph was extracted aseptically, and bacteria were isolated on NBTA agar, followed by purification on nutrient agar. Whole-genome sequencing, total DNA of INTA L402-1 strain was extracted using the Wizard Genomic DNA Purification Kit (Promega). The genome of the strain was sequenced using Illumina technology provided by the Unidad Operativa Centro Nacional de Genómica y Bioinformática (ANLIS Malbrán, Argentina). The genome assembly was performed de novo using SPAdes v3.15.4 genome assembler, and the resulting assembly underwent rigorous quality control assessment with QUAST v4.4 and CheckM v1.0.18 using KBase open-source software. Strain INTA L402-1 represents a putative novel species within the genus Stenotrophomonas based on genomic analyses, including average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and phylogenomic reconstruction.
Instituto de Microbiologia y Zoologia Agricola