Enhancing effects of exogenous bacteria on carbon fixation and water purification capacity of chlorella biofilms modified by MOFs
Microbial community analysis showed that the relative abundance of Pseudomonadotaincreased first and then decreased, reaching the highest level at the 1:0.5 ratio. This groupenriched structural-stabilizing genera of Pseudomonas and Exiguobacterium, while the 1:0.2and 1:1 groups contained more Vermamoeba that impeded biofilm construction. Functionalgene analysis further revealed that the 1:0.5 group upregulated genes associated with quorumsensing, environmental information processing, and glycolysis/gluconeogenesis, enhancingmicrobial carbon fixation. Stable phosphorus metabolism and energy transformation, togetherwith high abundances of nitrogen fixation and iron transport-related genes, effectivelyfacilitated nitrogen fixation and microalgae growth. These results confirmed the synergisticmechanism of optimal microalgae-bacteria ratio at the community and molecular levels.