Analysis of tRNA derived small non-coding RNAs generation during mitochondrial stress
tRNA derived small non-coding RNAs (tDRs) play important roles in stress response. However, their role in modulating organellar stress, and in particular mitochondrial stress, are less defined. Here, we exposed HEK293T cells to respiratory complex I inhibitor (Rotenone), repsiratory complex III inhibitor (Antimycin A), or to Arsenite as a general oxidative stressor. We sequenced small RNAs (<200nt) after deacetylation, demethylation, and end repair to generate profiles of tDRs expression during cellular response to mito-stress.