CUT&Tag-seq of eTreg cells from Bap1-deficient and wild-type mice and cultured etreg cells from wild-type mice
CUT&amp;Tag sequencing was performed to investigate the epigenetic mechanisms by which Bap1 regulate eTreg cell differentiation and maintenance. In the first experiment, eTreg cells were sorted from the lymph nodes of wild-type and Treg-specific Bap1 knockout mice and subjected to CUT&amp;Tag using an anti-H2AK119ub antibody to profile histone ubiquitination changes. In the second experiment, rTreg cells from WT mice were transduced with a Yy1-overexpression construct, cultured for 96 hours, and analyzed by CUT&amp;Tag using an anti-Yy1 antibody to identify Yy1-bound genomic regions. These datasets aim to elucidate the role of Bap1-mediated H2A deubiquitination and Yy1 recruitment in regulating eTreg differentiation and ferroptosis resistance.