CKMT2 Inhibits Oxidative Stress in Age-Related Hearing Loss by Activating the AMPK Pathway
Bioinformatic screening identified oxidative stress-related differentially expressed genes in ARHL, which were validated in ARHL rat models and cochlear basilar membrane (CBM) cells. RNA sequencing was performed to explore downstream mechanisms. Auditory function was assessed by auditory brainstem response (ABR) thresholds. Reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) levels were measured to determine oxidative stress. The AMP-activated protein kinase (AMPK) inhibitor Compound C was used to investigate pathway involvement.