Amplicon Analysis of Ulmus pumila Under Different Salinity Treatments
Healthy U. pumila branches were used as experimental materials. Branches with similar growth status and no visible disease or mechanical damage were collected from the same source population/family (F65212) to minimize background variation. Before treatment, the basal ends of the branches were freshly cut and transferred to hydroponic containers for acclimation under uniform conditions.The hydroponic experiment included four salinity treatments: a control treatment (CK, nutrient solution without additional NaCl) and three NaCl treatments, namely low salinity (25 mM NaCl), moderate salinity (50 mM NaCl), and high salinity (75 mM NaCl). Each treatment contained three independent containers as biological replicates, and each container included at least five U. pumila branches. The NaCl concentrations were established by dissolving the required amount of NaCl directly into the hydroponic solution.