DNAJC5 Stabilizes TAF15 by Competing with TRIM21 to Drive FASN-Dependent Lipogenesis in Ovarian Cancer
This project investigates the molecular mechanisms by which DNAJC5 promotes lipid metabolic reprogramming and tumor progression in ovarian cancer. We performed transcriptomic sequencing (RNA-Seq) on ovarian cancer cells with DNAJC5 overexpression and knockout to identify downstream transcriptional targets and signaling pathways. The aim is to elucidate how DNAJC5 stabilizes the transcription factor TAF15 by competing with the E3 ubiquitin ligase TRIM21, thereby driving FASN-dependent de novo lipogenesis. Our findings reveal a novel protein stability-metabolism axis and position the DNAJC5-TAF15-FASN cascade as a potential therapeutic target for lipid-dependent ovarian malignancies. Raw sequencing reads are deposited in the SRA to support the findings of this study.