Whole-genome resequencing of pear somaclonal variants
This project aims to characterize the mutational landscape of long-term subcultured pear using whole-genome sequencing.A total of 60 individual plants were used (30 per treatment group), with plants divided into six biological replicates per group, each replicate comprising a pooled sample of five individuals. Whole-genome sequencing libraries were prepared from 12 pooled samples (6 treatment + 6 control).Sequenced on the Illumina NovaSeq platform to identify high-impact polymorphisms associated with somaclonal variation.