Soil bacterial and fungal communities under sorghum green manure and carbonized rice husk in long-term muskmelon continuous cropping
This project characterizes pre-plant soil bacterial and fungal communities in an 11-year muskmelon continuous-cropping field in Beihai, Guangxi, China. A randomized complete block experiment compared a continuous-cropping control (CK), sorghum green manure (FN), and sorghum green manure plus carbonized rice husk (TN), with three field replicates per treatment. Soil was sampled on 10 September 2025 after sorghum incorporation and before establishment of the subsequent muskmelon crop. PacBio Sequel II full-length bacterial 16S rRNA gene and fungal ITS amplicon sequencing was used to assess amendment-conditioned soil microbial community responses.