Single-cell transcriptomic comparison of unmodified NK cells and polymer-armed NK cells
To investigate whether surface modification with multivalent galectin-3-binding polymers alters the transcriptional landscape of NK cells, we performed single-cell RNA sequencing on unmodified murine NK cells and a-NKs (NK cells armed with polymeric arms). This dataset enables comparative analysis of global gene expression profiles, including key activating receptors (NKG2D, NKp46, DNAM-1) and inhibitory receptors (NKG2A, Ly-49A), between the two groups. The results provide insights into the transcriptional consequences of polymer-based cell engineering and support the conclusion that surface modification does not induce global transcriptional changes. Raw sequence data are available for further analysis.