Transcriptomic profiling of VIbrio cholerae-infected intestinal epithelial cells
TagA is a toxT-dependent secreted metallo-protease expressed by Vibrio cholerae. The goal of the study is to assess TagA-dependent transcriptional shifts that occur in intestinal epithelial cells infected with Vibrio cholerae. To that end, LS174T cells (intestinal epithelial cell line) were infected with wild-type or del-tagA Vc cells constitutively expressing ToxT, followed by RNA isolation, cDNA library preparation and sequencing.