16S rRNA V3-V4 Sequencing of Clinical Nasopharyngeal Swab Specimens with Confirmed Pathogen Detection
Clinical remnant nasopharyngeal swab samples were subjected to 16S rRNA gene sequencing of the V3-V4 region to characterize microbial community composition. Specimens were selected from a cohort with confirmed pathogen detection, including both viral and bacterial organisms, identified by total RNA sequencing. Sequencing was performed on the Illumina MiSeq platform using 2x300 bp paired-end reads. Raw sequence data were used for downstream microbial profiling analyses.