Genome of Enterococcus faecalis isolated from 5-FU treated cancer patients(3rd generation sequence data)
5-Fluorouracil (5-FU), a widely used first-line anticancer drug, functions primarily as a pyrimidine analog that undergoes multiple intracellular conversions to inhibit thymidylate synthase, leading to cell death.we isolated Enterococcus faecalis from 5-FU treated cancer patients, and found 5-FU-resistant and sensitive E.faecalis isolations.We performed whole-genome sequencing on six 5-FU-resistant and six 5-FU-sensitive E.faecalis isolates.The genome of the 5-FU-resistant strain was 2,840,722 bp in size, containing 2,666 genes, including 12 rRNA genes, 15 sRNA genes, and 58 tRNA genes. Additionally, it harbored two plasmids of approximately 73 kb and 55 kb. In contrast, the genome of the 5-FU-sensitive strain was 3,244,422 bp, containing 3,237 genes, including 12 rRNA genes, 15 sRNA genes, and 65 tRNA genes, plus two plasmids of approximately 65 kb and 5 kb.