Persistent rhizosphere bacterial restructuring contrasts with unstable grain Cd mitigation by biochar across successive rice cultivation cycles
This project investigates the effects of biochar amendment on rhizosphere microbial communities and cadmium dynamics in rice across two successive cultivation cycles. Two rice cultivars were grown in cadmium-contaminated soil with or without Paulownia-derived biochar, and rhizosphere samples were collected at tillering, jointing, booting, and maturity stages. Bacterial 16S rRNA gene and fungal ITS amplicon sequencing was performed to characterize temporal and cultivar-dependent changes in rhizosphere microbial communities and their relationships with soil properties and cadmium transfer from soil to rice.