RNA-seq analysis of Aspergillus oryzae RIB40 under different carbon source conditions with a focus on lactate assimilation
This project aims to investigate transcriptional responses of Aspergillus oryzae RIB40 associated with lactate assimilation. We observed that the RIB40 strain does not efficiently utilize lactate when cultured in a medium containing 0.5% lactate as the sole carbon source (Lac condition), whereas lactate utilization is enabled in the presence of a small amount of glucose (Lac + 0.1% Glu condition). To characterize transcriptional changes under these conditions, RNA-seq analysis was performed using mycelia cultivated in Lac for 4 days. In addition, to investigate transcriptional profiles under Lac + 0.1% Glu conditions, mycelia were collected at day 1 (D1), day 3 (D3), and day 5 (D5) and subjected to RNA-seq analysis. As a control, mycelia cultivated in glucose-containing medium (CD) were also analyzed.