Transcriptomic Profiling of MDK-Modulated SCLC and CAR-T Cell Models
RNA sequencing (RNA-seq) was performed to characterize transcriptomic alterations in multiple experimental models of small cell lung cancer (SCLC). Specifically, we profiled: (1) tumor tissues from MDK-knockdown and control RPM SCLC mouse models; (2) MDK-knockdown and negative control human SCLC cell lines (NCI-H146/SHP-77); (3) sMDK-DLL3 CAR-T cells and control CAR-T cells after co-culture with SHP-77 cells; and (4) sMDK-DLL3 CAR-T cells and control CAR-T cells under non-co-culture conditions. Total RNA was extracted using TRIzol reagent, library construction was conducted with NEBNext Ultra RNA Library Prep Kit, and sequencing was performed on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads to identify differentially expressed genes and biological pathway alterations.