The Complete Chloroplast Genome of Chlorophytum nepalense Analysis and Phylogenetics
n this study, we sequenced, assembled, and annotated the complete chloroplast genome of C. nepalense to characterize its structural features and establish its phylogenetic position within Liliaceae. The chloroplast genome is 154,291 bp in length and exhibits the canonical quadripartite organization typical of angiosperms, comprising a large single-copy (LSC) region of 83,807 bp, a small single-copy (SSC) region of 17,192 bp, and a pair of inverted repeats (IRa and IRb) of 26,256 bp each, with an overall GC content of 37.7%. The genome encodes 132 annotated genes, including 87 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. Maximum likelihood phylogenetic inference based on complete chloroplast genome sequences robustly places C. nepalense within a well-supported monophyletic Chlorophytum clade, clearly distinguishing it from morphologically convergent genera such as Ophiopogon and Agave.