Transcriptome analysis of muscle tissue of triploid crucian carp (Carassius auratus)
This study investigates the regulatory effects of dietary glutamine supplementation at three concentrations, 0%, 1%, and 2%, designated as CON, GLN1, and GLN2, respectively on muscle quality traits of triploid crucian carp. We used 90 to 110 g juvenile fish as experimental subjects, with 3 biological replicates per treatment group, yielding a total of 9 skeletal muscle samples for transcriptomic profiling. All samples were sequenced to identify differentially expressed genes associated with flavor substance synthesis, textural properties, and antioxidant capacity. The overarching aim of this work is to clarify the molecular mechanisms by which dietary glutamine modulates muscle quality in this commercially important aquaculture species, providing a genetic basis for optimizing feed formulations for triploid crucian carp production. All raw sequencing data and associated metadata generated in this study have been submitted to the NCBI Sequence Read Archive for public access.