XNA Sequencing by Chemical Ligation of DNA Fragments using XNA as a Template
This project aims to establish a nonenzymatic method for transferring sequence information from acyclic artificial nucleic acid L-aTNA to DNA. DNA fragments were chemically ligated on L-aTNA templates, and the resulting DNA products were amplified and analyzed by high-throughput sequencing. The sequencing data were used to evaluate the accuracy and sequence fidelity of L-aTNA-to-DNA information transfer, providing a basis for XNA sequencing.