single nucleus RNAseq of liver samples
Single-nucleus RNA-seq reveals hepatocyte heterogeneity and progenitor-like populations in Lamtor2-deficient mouse liver Single-nucleus RNA sequencing (snRNA-seq) was performed on whole livers from control mice and from two models of hepatocyte-specific Lamtor2 deletion (a chronic developmental knockout and an acute adult knockout). Unsupervised transcriptomic analysis identified multiple liver cell types and hepatocyte subpopulations, revealing that early (developmental) loss of Lamtor2 triggers the emergence of hepatocytes with a biliary/progenitor-like gene expression signature, whereas acute adult deletion leads to a distinct hepatocyte subcluster with altered metabolic gene expression. These findings highlight hepatocyte plasticity and metabolic reprogramming in Lamtor2-deficient livers, underscoring the crucial role of the Ragulator/mTORC1 signaling complex in maintaining normal hepatocyte identity and function.
Single-nucleus RNA-seq reveals hepatocyte heterogeneity and progenitor-like populations in Lamtor2-deficient mouse liver Single-nucleus RNA sequencing (snRNA-seq) was performed on whole livers from control mice and from two models of hepatocyte-specific Lamtor2 deletion (a chronic developmental knockout and an acute adult knockout). Unsupervised transcriptomic analysis identified multiple liver cell types and hepatocyte subpopulations, revealing that early (developmental) loss of Lamtor2 triggers the emergence of hepatocytes with a biliary/progenitor-like gene expression signature, whereas acute adult deletion leads to a distinct hepatocyte subcluster with altered metabolic gene expression. These findings highlight hepatocyte plasticity and metabolic reprogramming in Lamtor2-deficient livers, underscoring the crucial role of the Ragulator/mTORC1 signaling complex in maintaining normal hepatocyte identity and function.