PiggyBac-mediated transgenesis and whole genome sequencing in sheep
This study evaluated piggyBac-mediated transgenesis using pronuclear injection in both in vivo- and in vitro-derived sheep embryos and assessed cytoplasmic injection as an alternative approach . Whole-genome sequencing of the healthy founder male produced by cytoplasmic injection was performed to identify genomic integration sites and confirm single-copy insertion within a non-coding RNA locus