Intratumoral mycobiome in lung adenocarcinoma revealed by ITS amplicon sequencing
This study investigated the intratumoral mycobiome in lung adenocarcinoma using ITS amplicon sequencing. Paired tumor tissues and adjacent normal lung tissues were collected from patients with pathologically confirmed lung adenocarcinoma. Adjacent normal tissues were obtained at least 5 cm from the tumor margin. Negative controls, including extraction controls and sampling controls, were included to monitor potential environmental and reagent contamination. The generated paired-end sequencing data were used to characterize fungal community composition and compare mycobiome profiles between tumor and adjacent normal tissues.