Effects of Helicobacter pylori infection and dietary choline on the gut microbiota of mice
This project investigates the effects of Helicobacter pylori infection and dietary choline on the gut microbiota of male SPF C57BL/6J mice. Mice were assigned to six groups receiving a normal diet, 1% choline diet, or 3% choline diet, with or without H. pylori infection, and were sampled after 8 or 16 weeks. Cecal contents were collected from six mice per group at each time point. The V3-V4 region of the bacterial 16S rRNA gene was sequenced using the Illumina MiSeq paired-end platform to characterize changes in gut microbial community composition.