Placenta CUTTag Analysis Reveals Post-transcriptional Dysregulation and Reduced FXR Binding in a PCOS mouse model
To validate whether Nr1h4/FXR can directly regulate downstream targets, including those bile acid-related transporters in the placenta, we performed genome-wide Cleavage Under Targets and Tagmentation (CUTTag) sequencing analysis, a recently developed method for profiling protein-DNA interactions with high resolution and low input requirements. Using an antibody specific to Nr1h4/FXR, we identified widespread FXR binding events across the mouse placenta genome.