RNA-seq of rat dental pulp tissue in experimental pulpitis model at 3 and 7 days post-pulp exposure
This project aims to investigate the transcriptomic changes in inflamed dental pulp tissue during the progression of pulpitis. Experimental pulpitis was induced in the maxillary first molars of male Sprague-Dawley rats (8 weeks old) by pulp exposure using a #1/4 round bur and #10 K-file under sterile conditions. Untreated contralateral molars served as healthy controls. Pulp tissues were collected at 3 and 7 days post-intervention (n = 3 per group per time point). Total RNA was extracted and subjected to high-throughput RNA sequencing to identify differentially expressed genes and signaling pathways associated with pulpitis development and progression. This dataset provides a valuable resource for understanding the molecular mechanisms of dental pulp inflammation.