Evaluation of a simplified protocol for water-insoluble drugs in the EUCAST AMST reference method
Accurate MIC testing by broth microdilution is becoming increasingly important for determining tuberculosis drug susceptibility. This is especially true for newer or repurposed drugs that need to be dissolved in DMSO due to limited solubility in water. One challenge is that DMSO concentrations can vary across wells in plate-based dilution series, making results harder to interpret and reproduce.  In our study, we tested a simplified microdilution layout that keeps DMSO levels constant and follows the EUCAST AMST reference method.
Accurate MIC testing by broth microdilution is becoming increasingly important for determining tuberculosis drug susceptibility. This is especially true for newer or repurposed drugs that need to be dissolved in DMSO due to limited solubility in water. One challenge is that DMSO concentrations can vary across wells in plate-based dilution series, making results harder to interpret and reproduce.  In our study, we tested a simplified microdilution layout that keeps DMSO levels constant and follows the EUCAST AMST reference method.