Transcriptome sequencing of Delphinieae and related outgroups for phylogenomics and ancestral whole-genome duplication analysis
This project contains RNA-seq transcriptome data generated from silica gel-dried leaf material of Delphinieae and closely related outgroups in Ranunculaceae. The data were produced to reconstruct the deep phylogeny of Delphinieae, evaluate the causes of gene tree conflict at backbone nodes, estimate divergence times, and investigate whether an ancestral whole-genome duplication is associated with the origin and evolution of complex bilaterally symmetrical flowers in the tribe. Total RNA was extracted from field-collected samples, cDNA libraries were constructed, and paired-end sequencing was performed on the Illumina NovaSeq 6000 platform. These transcriptomic resources provide a basis for phylogenomic, comparative, and evolutionary studies of Delphinieae, including analyses of floral trait evolution, genome duplication history, and lineage diversification.