Oxford Nanopore PromethION whole-genome sequencing of the invasive zebra mussel (Dreissena polymorpha)
Long-read whole-genome sequencing of the zebra mussel (Dreissena polymorpha), an invasive freshwater bivalve, generated to support de novo assembly of a draft reference genome. Genomic DNA from a specimen collected in Wisconsin, USA was sequenced on an Oxford Nanopore PromethION (R9.4.1 flow cell, ligation-based native DNA preparation) in a single run in early 2019, yielding 13,223,086 reads and 54.4 Gb of sequence (42.6 Gb passing filter; mean read length 4.1 kb, longest read 179 kb), corresponding to approximately 32-fold coverage of the ~1.7 Gb genome. This study comprises the raw nanopore signal data (multi-read FAST5), deposited so that the reads can be re-basecalled with current models and reused independently of the basecalls available at the time of sequencing. The data were generated as part of work toward a draft genome assembly for this species to support comparative genomics, invasion genetics, and the evaluation of genetic biocontrol strategies for invasive dreissenid mussels. The dataset is released without restriction for unrestricted reuse.
Long-read whole-genome sequencing of the zebra mussel (Dreissena polymorpha), an invasive freshwater bivalve, generated to support de novo assembly of a draft reference genome. Genomic DNA from a specimen collected in Wisconsin, USA was sequenced on an Oxford Nanopore PromethION (R9.4.1 flow cell, ligation-based native DNA preparation) in a single run in early 2019, yielding 13,223,086 reads and 54.4 Gb of sequence (42.6 Gb passing filter; mean read length 4.1 kb, longest read 179 kb), corresponding to approximately 32-fold coverage of the ~1.7 Gb genome. This study comprises the raw nanopore signal data (multi-read FAST5), deposited so that the reads can be re-basecalled with current models and reused independently of the basecalls available at the time of sequencing. The data were generated as part of work toward a draft genome assembly for this species to support comparative genomics, invasion genetics, and the evaluation of genetic biocontrol strategies for invasive dreissenid mussels. The dataset is released without restriction for unrestricted reuse.