Bulk RNA-sequencing of C57BL/6J bone marrow stromal cells stained with anti-Neo1 or anti-TrkB antibodies
The aim of this study was to investigate mouse bone marrow stromal cells expressing cell-surface Neo1 or TrkB. For this, we isolated cells from digested bone, depleted hematopoietic populations, and performed FACS for subsequent low-input total RNA-sequencing. We compared gene expression profiles of sorted Neo1+ or TrkB+ cells vs. Neo1- or TrkB- cells, respectively. The animals used were female 13-week-old C57BL/6J mice.
The aim of this study was to investigate mouse bone marrow stromal cells expressing cell-surface Neo1 or TrkB. For this, we isolated cells from digested bone, depleted hematopoietic populations, and performed FACS for subsequent low-input total RNA-sequencing. We compared gene expression profiles of sorted Neo1+ or TrkB+ cells vs. Neo1- or TrkB- cells, respectively. The animals used were female 13-week-old C57BL/6J mice.