16S rRNA amplicon sequencing of bovine milk microbiota during clinical mastitis and seasonal heat stress
This dataset contains high-throughput 16S rRNA gene amplicon sequencing (V3 - V4 hypervariable region) data derived from bovine milk samples collected in a subtropical climate. The project investigates the interactive impact of intramammary infection (healthy control vs. clinical mastitis) and macroclimatic seasonality (summer heat stress vs. winter) on the structural stability and taxonomic composition of the bovine milk microbiome. Raw sequencing reads were generated to evaluate mammary gland dysbiosis, the depletion of core commensal taxa, and season-dependent shifts in mastitis pathogen etiology.