16S rRNA gene amplicon sequencing of rice root and shoot endosphere bacterial communities under a treatment gradient
This project characterizes bacterial endophytic communities associated with roots and shoots of Oryza sativa across six experimental treatment levels (0, 0.1, 1, 10, 20 and 50) with three biological replicates per tissue and level. The bacterial 16S rRNA gene V3-V4 region was amplified using barcoded 341F/806R-family primers and sequenced as paired-end reads on an Illumina platform. The dataset contains 36 biological samples and supports comparison of root and shoot endosphere communities across the experimental gradient.