SWGA and Genome Assembly of Helicobacter pylori from Gastric Biopsy Specimens of Dyspeptic Patients
This project aims to reconstruct draft genomes of Helicobacter pylori directly from gastric biopsy specimens using selective whole genome amplification (sWGA) followed by Oxford Nanopore sequencing and de novo assembly. Samples were obtained from dyspeptic patients and represent low-biomass clinical specimens in which conventional culture and diagnostic assays may underestimate the presence of H. pylori. The study focuses on genomic characterization of antimicrobial resistance determinants and virulence-associated genes in biopsy-derived H. pylori genomes. These data support investigation of phenotypically negative but molecularly detectable infections and provide insights into strain diversity and potential transmission dynamics. All genomes were assembled from sWGA-enriched DNA extracted from gastric biopsy samples and analyzed within a clinical research framework.