Mus musculus strain:C57BL6/J Transcriptome or Gene expression
Transcriptome analysis was performed on transgenic IL-37a mice transgenic mice splenocytes. Splenocytes of WT C57BL6/J and IL-37aTg mice were seeded in 24 wells cell culture plate . These cells were stimulated with 500 ng/ml LPS for 5 hours. Total RNA from each sample was isolated and purified. Construction of cDNA libraries and next-generation sequencing was performed.Raw data from RNA-seq were analysed for each group . The average RPKM (Reads Per Kilobase Million) per group was estimated. Moreover, the p-value and fold-changes were analysed for comparisons between IL-37aTg and WT.