WES for analysis of subclonal composition in a M. musculus melanoma
In order to study the interactions of subclones with contrasting proliferative and invasive phenotypes in melanoma, two B2905 melanoma sublines representing these two distinct phenotypes were admixed at different ratios (100%/0%, 80%/20%, 50%/50%, 20%/80%, 0%/100% for proliferative/invasive, respectively) and a total of 1.0 x 10^6 cells were implanted into the flank of 6 female athymic nu/nu (nude) or C57BL/6N (B6) mice in each admixture group. Three tumors from each admixture group were harvested at different sizes (approximately 200mm^3, 500mm^3, and 1000mm^3). These samples were homogenized for preparing exome libraries using Agilent SureSelect XT Mouse All Exon kit, and the libraries were sequenced on one NovaSeq HiSeq run with paired-end sequencing mode.