Mechanistic Study of Biochar Amendment on Soil Microbiome in Tobacco Fields
This study investigates how tobacco stalk biochar modulates soil and rhizosphere microbial communities to improve tobacco growth under reduced chemical fertilization. A pot experiment included two treatment sets: full fertilizer with three biochar application rates (40B, 80B, 160B), and reduced fertilizer (5 %, 10 %, 15 % , less) combined with 80B biochar. Soil physicochemical properties, enzyme activities, microbial communities, and tobacco growth related traits (biomass, nutrient uptake) were characterized. We aim to disentangle direct (soil habitat improvement) and indirect (microbiome mediated) biochar effects, and evaluate whether biochar offsets fertilizer reduction. This work supports sustainable tobacco waste recycling and precision fertilization for tobacco planting soils.