Direct RNA sequencing of HTLV-1 transcripts in control and HIV-1-infected MT-2 cells with ALKBH5 treatment
This project investigates the post-transcriptional characteristics of integrated human T-lymphotropic virus type 1 (HTLV-1) transcripts in MT-2 cells under HIV-1 infection and ALKBH5-mediated RNA demethylation. Total RNA from both control MT-2 cells and HIV-1-infected MT-2 cells was subjected to either mock treatment or ALKBH5 treatment, followed by Oxford Nanopore direct RNA sequencing. The dataset enables comparative analysis of HTLV-1 transcript composition, alternative splicing, poly(A)-tail length, and RNA modification patterns, particularly m6A-associated signals, across control and HIV-1-infected conditions before and after ALKBH5-mediated demethylation.