DNA barcoding (COI) of scavenging amphipods in Saltfjord and Skjerstadfjord.
This study generated mitochondrial cytochrome oxidase I (COI) amplicon sequences from 417 marine amphipods collected from two sub-Arctic fjords (Saltfjord and Skjerstadfjord). Amplicons were sequenced using Illumina sequencing (PE 150) to generate DNA barcode data for species identification, and biodiversity assessment. Four species of Lysianassids were identified: Tmetonyx cicada (Fabricius, 1780), Scopelocheirus hopei (Costa, 1851), Orchomenella obtusa (G. O. Sars, 1895), and Anonyx lilljeborgi Boeck, 1871. Two hundred individuals of Orchomenella obtusa were used for study of population differentiation between the two fjords using amplicon sequences of COI and 18S rRNA.
This study generated mitochondrial cytochrome oxidase I (COI) amplicon sequences from 417 marine amphipods collected from two sub-Arctic fjords (Saltfjord and Skjerstadfjord). Amplicons were sequenced using Illumina sequencing (PE 150) to generate DNA barcode data for species identification, and biodiversity assessment. Four species of Lysianassids were identified: Tmetonyx cicada (Fabricius, 1780), Scopelocheirus hopei (Costa, 1851), Orchomenella obtusa (G. O. Sars, 1895), and Anonyx lilljeborgi Boeck, 1871. Two hundred individuals of Orchomenella obtusa were used for study of population differentiation between the two fjords using amplicon sequences of COI and 18S rRNA.