Transcriptomic dynamics of the seagrass Zostera marina under high-temperature stress
Uniform healthy Zostera marina seedlings, with five individuals per 3 L sterile glass bottle, were incubated in sterilized seawater for 18 days under controlled laboratory conditions. Three experimental groups were designed: (1) HWC (Control group): Seedlings were cultured at a water temperature of 20-24 C, with no additional treatments. (2) HWK (High-temperature stress group): Seedlings were cultured at a constant water temperature of 28-32 C. (3) HWW (High-temperature + microbial inoculation group): Seedlings were cultured at 28-32 C, and E. aurantiacum strain S11 was inoculated into the culture seawater to a final density of approximately 10^4 CFU/mL. All experimental culture systems were sealed to prevent water evaporation, and all groups were maintained under the same environmental conditions applied during the pre-experiment seedling acclimation period.