Two novel Clostridium sp. species with PLC homologs
This project comprises complete and near-complete genome sequences of Clostridium sp. strains LIBA-8541 (DSM 14804) and LIBA-8841 (DSM 14803), recovered from human clinical specimens in Costa Rica. Genomes were generated by PacBio Sequel II long-read sequencing and polished with matched Illumina reads. Average nucleotide identity and digital DNA-DNA hybridization values place both strains below accepted species thresholds relative to C. perfringens and to each other, indicating two distinct novel species-level lineages within the genus. Both genomes encode a divergent homolog of the C. perfringens alpha-toxin (phospholipase C) together with additional putative virulence determinants. These data support the taxonomic characterization of the isolates and address the risk of their misidentification as C. perfringens by routine diagnostic methods.
This project comprises complete and near-complete genome sequences of Clostridium sp. strains LIBA-8541 (DSM 14804) and LIBA-8841 (DSM 14803), recovered from human clinical specimens in Costa Rica. Genomes were generated by PacBio Sequel II long-read sequencing and polished with matched Illumina reads. Average nucleotide identity and digital DNA-DNA hybridization values place both strains below accepted species thresholds relative to C. perfringens and to each other, indicating two distinct novel species-level lineages within the genus. Both genomes encode a divergent homolog of the C. perfringens alpha-toxin (phospholipase C) together with additional putative virulence determinants. These data support the taxonomic characterization of the isolates and address the risk of their misidentification as C. perfringens by routine diagnostic methods.