Nanopore Direct RNA Sequencing of HTLV-1 Transcripts in HIV-1-Infected MT-2 Cells
This project investigates the effects of HIV-1 infection on the post-transcriptional processing of HTLV-1 transcripts in MT-2 cells. Total RNA from HIV-1-infected MT-2 cells was subjected to Oxford Nanopore direct RNA sequencing. Quality-filtered reads were aligned to the HTLV-1 reference genome (GenBank accession AF033817.1) to characterize viral transcript profiles, including splice-junction usage, transcript isoforms, poly(A)-tail features, and RNA modification signals. The deposited BAM files contain HTLV-1-aligned sequencing reads and are provided to support analyses of HTLV-1 RNA processing following HIV-1 infection.