Kavaratti reef lagoon eDNA fish diversity assessment using MiFish primers Raw sequence reads
This project dataset contains raw high-throughput sequencing reads generated from an environmental DNA (eDNA) metabarcoding survey of ichthyofaunal diversity across the coral reef lagoon of Kavaratti Island, Lakshadweep Archipelago, India.Seawater samples collected across distinct lagoon habitats were filtered to extract ambient eDNA. The hypervariable region of the mitochondrial 12S rRNA gene was amplified using teleost- and elasmobranch-specific MiFish universal primers to comprehensively profile the local fish community. The resulting amplicon libraries were sequenced on an Illumina platform to generate paired-end raw sequence reads.