RNA sequencing of 16 monoclonal strains of Alexandrium minutum for gene expression analysis related to PST production for the DIFAMin project, 2021.
The study was performed to determine the genes differentially expressed by PST producing and non-producing strains of A. minutum. Strains were grown in K+Na2SiO3 medium, in an algal incubator (12/12 L:D cycle, 18C, 7 days), in 5 replicates. Cell pellets were lysed by sonication on ice. RNA was extracted using a NucleoSpin TriPrep kit (Macherey-Nagel). RNA was stored at -80C. Library preparation was performed using the Illumina TruSeq mRNA stranded kit starting from 0.5 g of total RNA. Library quality and quantity were assessed on a Bioanalyzer (Agilent Technologies, Palo Alto, CA) using high-sensitivity DNA analysis chips. Paired-end sequencing (2x150bp) of the 79 libraries was performed on two lanes of Flowcell SP on Illumina NovaSeq6000 at the GeT-PlaGe France Genomics sequencing platform (Toulouse, France).
The study was performed to determine the genes differentially expressed by PST producing and non-producing strains of A. minutum. Strains were grown in K+Na2SiO3 medium, in an algal incubator (12/12 L:D cycle, 18C, 7 days), in 5 replicates. Cell pellets were lysed by sonication on ice. RNA was extracted using a NucleoSpin TriPrep kit (Macherey-Nagel). RNA was stored at -80C. Library preparation was performed using the Illumina TruSeq mRNA stranded kit starting from 0.5 g of total RNA. Library quality and quantity were assessed on a Bioanalyzer (Agilent Technologies, Palo Alto, CA) using high-sensitivity DNA analysis chips. Paired-end sequencing (2x150bp) of the 79 libraries was performed on two lanes of Flowcell SP on Illumina NovaSeq6000 at the GeT-PlaGe France Genomics sequencing platform (Toulouse, France).