DNA methylation profiling of antibiotics-treated and untreated brown planthopper (BPH) insects
The predicted CpG islands within the stress-responsive genes in BPH were PCR-amplified from the bisulfite-treated genomic DNA, extracted from antibiotics-treated BPH individuals. The fragments thus obtained were gel purified and sequenced, using the NEBNext Ultra DNA Library Prep Kit, on an Illumina MiSeq next generation sequencing system, using MiSeq Reagent Kit v2 500 cycles chemistry.