Evian water metagenome dataset
Maintaining the biostability of unchlorinated drinking water is critical for health protection, yet the roles of biofilms and planktonic bacteria remain poorly understood. This study evaluated biofilm detachment in hydraulically stable drinking water systems during changes in water sources and its impact on microbial diversity and water quality. Also, we investigated the influence of biofilm detachment on microbial diversity in drinking water under various water-source conditions and examined the potential impact of microorganisms detached from biofilms on drinking water quality and safety following changes in drinking water resources. To evaluate biofilm detachment, a pilot-scale drinking water supply system was developed, grab and online drinking water quality measurements were performed, and metagenomic analysis of microbiological elements was conducted. The results demonstrated that biofilm separation and changes in microbial composition within the pilot-scale loop were independent of water flow rate and hydraulic parameters, while metagenomic analyses of Shannon and Simpson diversity indices indicated no statistically significant differences in microbial community composition between Evian water and samples collected during the pilot study, with p-values for the Shannon index ranging from 0.3 to 1.0 and for the Simpson index from 0.6 to 1.0. These results suggest that key taxa and their relative abundances shift over time when drinking water is introduced into the system, and that these changes are primarily driven by biofilm detachment.
Maintaining the biostability of unchlorinated drinking water is critical for health protection, yet the roles of biofilms and planktonic bacteria remain poorly understood. This study evaluated biofilm detachment in hydraulically stable drinking water systems during changes in water sources and its impact on microbial diversity and water quality. Also, we investigated the influence of biofilm detachment on microbial diversity in drinking water under various water-source conditions and examined the potential impact of microorganisms detached from biofilms on drinking water quality and safety following changes in drinking water resources. To evaluate biofilm detachment, a pilot-scale drinking water supply system was developed, grab and online drinking water quality measurements were performed, and metagenomic analysis of microbiological elements was conducted. The results demonstrated that biofilm separation and changes in microbial composition within the pilot-scale loop were independent of water flow rate and hydraulic parameters, while metagenomic analyses of Shannon and Simpson diversity indices indicated no statistically significant differences in microbial community composition between Evian water and samples collected during the pilot study, with p-values for the Shannon index ranging from 0.3 to 1.0 and for the Simpson index from 0.6 to 1.0. These results suggest that key taxa and their relative abundances shift over time when drinking water is introduced into the system, and that these changes are primarily driven by biofilm detachment.