RNA sequencing of BV2 microglia following coculture with Th1 and Th17 cells
The goal of this study was to investigate how pathogenic Th1 and Th17 cells influence the transcriptional profile of microglia under inflammatory conditions that resemble the central nervous system (CNS) environment in multiple sclerosis (MS). An in vitro coculture system was established using BV2 microglia and Th1 and Th17 cells differentiated from experimental autoimmune encephalomyelitis (EAE) mice. RNA sequencing was performed to characterize gene expression changes in BV2 microglia following coculture. Differential expression and pathway enrichment analyses demonstrated activation of immune disease-related pathways and significant enrichment of MS-associated microglial gene signatures. These data provide a resource for understanding T cell-microglia interactions and the molecular mechanisms underlying microglial activation in neuroinflammatory diseases such as MS.