Whole-genome sequencing of Paenibacillus macerans isolates from the gut of mice colonized with barcoded Escherichia coli under spectinomycin selection
This BioProject contains Oxford Nanopore whole-genome sequencing of fourPaenibacillus macerans isolates cultured from the feces of mice colonized with achromosomally barcoded Escherichia coli MG1655 library under continuous spectinomycinselection.In the parent experiment, mice were pretreated with a broad-spectrum antibioticcocktail, gavaged with barcoded E. coli, and maintained under spectinomycin. 16S rRNAprofiling showed that a Paenibacillaceae population, present at low relative abundancein the antibiotic-only control mice, expanded to become a dominant member of thecommunity in every colonized mouse. One isolate was recovered from each of the fourmice of the second colonized cohort and sequenced to determine whether this expansionwas associated with acquisition of a spectinomycin resistance determinant.Long-read sequencing yielded single-contig, closed chromosome assemblies ofapproximately 7.47 Mb for three of the four isolates, each estimated by CheckM at99.85 percent complete with 0.12 percent contamination. The fourth isolate wassequenced at low coverage and assembled into seven contigs. All four are assigned toPaenibacillus macerans and share approximately 99.3 percent average nucleotideidentity with the Paenibacillus macerans type-strain reference genomeGCF_022494515.1.