CROP-seq analysis of CRISPR perturbations in Mac2a cells
Mac2a cells (anaplastic large-cell lymphoma) expressing sgRNAs targeting hit genes identified in genome-wide CRISPR-Cas9 screens were subjected to CRISPR droplet sequencing (CROP-seq). Samples were multiplexed using TotalSeq hashtag oligonucleotides to enable assignment of drug treatment conditions. This dataset contains raw gene expression and hashtag library sequencing data used to assess changes in gene expression after hit gene silencing and drug treatments at single-cell resolution.