Comparative 16S rRNA gut microbiome profiling of 5-azacytidine-treated and untreated brown planthopper (BPH) insects
16S rRNA gut-microbiome profiling was carried out for BPH insects treated with 5-azacytidine. Genomic DNA from treated and untreated insects was PCR amplified using barcoded 16S rRNA gene-specific primers. The fragments thus obtained were subjected to 16S rDNA Amplicon Metagenomic Sequencing on a NovaSeq PE250 sequencing platform.