Whole-genome sequencing and variant discovery in the grey wolf (Canis lupus)
The main goal of this study was to detect SNPs in wolf genomes based on publicly available and newly acquired data. The DNA of 15 Polish wolves was isolated from tissue, libraries were constructed using the TruSeq DNA Nano Kit (Illumina), and sequencing was performed on an Illumina NovaSeq 6000. Additionally, raw reads from 284 wolves from other regions were downloaded from publicly available databases. The raw reads were mapped to the wolf reference genome. Base quality score recalibration and variant detection were conducted using the Genome Analysis Toolkit. The obtained variants were used to analyze the genetic structure and differentiation between wolf populations and to estimate the basic genetic diversity parameters within the studied populations.