RNA-seq and CUT&Tag profiling of UCH2-mediated H2A.Z deubiquitination during phosphate starvation in Arabidopsis thaliana
This BioProject contains raw sequencing data for genome-wide RNA-seq and CUT&amp;Tag experiments investigating the role of the ubiquitin C-terminal hydrolase UCH2 in chromatin and transcriptional responses to phosphate (Pi) starvation in Arabidopsis thaliana (Col-0). Whole-seedling samples were collected from wild-type Col-0 and uch2 mutant plants under control (P+) and low-phosphate (P-) conditions. RNA-seq: four groups (Col-0 P+, Col-0 P-, uch2 P+, uch2 P-) with n = 3 biological replicates per group (12 samples). CUT&amp;Tag: (1) H2A.Z antibody profiling in Col-0 and uch2 under P+ and P- (four groups, n = 2 replicates each; 8 samples); (2) UCH2-FLAG profiling in Col-0 (P+ and P-) and phr1 mutant (P-) (three groups, n = 2 replicates each; 6 samples); (3) PHR1-FLAG profiling in Col-0 under P- (one group, n = 2 replicates; 2 samples). In total there are 28 samples. These datasets support integrated analysis of UCH2 association, H2A.Z occupancy, and transcriptome changes during phosphate starvation.