16S rRNA amplicon sequencing of fecal microbiota from Tibetan pigs fed fermented highland barley distiller's grains
This study investigated the effects of dietary supplementation with fermented highland barley distiller's grains (FBG) on growth performance, nutrient apparent digestibility, blood physiological and biochemical indices, intestinal morphology, organ indices, fecal microbiota, and economic benefits of Tibetan pigs. A total of 40 healthy castrated male Tibetan pigs with initial body weight of (16.18 +/- 1.95) kg were randomly assigned to four groups (10 replicates per group, 1 pig per replicate) in a completely randomized block design. The four groups were fed a basal diet (CK group) or diets supplemented with 10% (L-FBG), 20% (M-FBG), and 30% FBG (H-FBG), respectively. Fecal samples were collected at the end of the 35-day feeding trial for 16S rRNA gene amplicon sequencing (V3-V4 region) using the Illumina NovaSeq platform. The raw sequence data were deposited in the NCBI Sequence Read Archive (SRA) under this BioProject accession. The data will be used to evaluate the effects of FBG supplementation on gut microbial diversity and community composition of Tibetan pigs.