16S rRNA gene amplicon sequencing of fecal microbiota from DSS-induced ulcerative colitis C57BL/6 mice intervened with Radix Hedysari polysaccharide HPS-80
Ulcerative colitis (UC) is a chronic inflammatory bowel disease closely associated with gut microbiota dysbiosis. Radix Hedysari polysaccharide-80 (HPS-80) is a bioactive polysaccharide fraction derived from Hedysarum polybotrys Hand.-Mazz., which exerts preventive effects against dextran sulfate sodium (DSS)-induced ulcerative colitis in C57BL/6 mice. To characterize gut microbial alterations upon HPS-80 intervention, we performed 16S rRNA gene amplicon sequencing targeting the V3-V4 hyper-variable region. Paired fecal samples were collected from four experimental groups (Control, DSS model, 5-ASA positive control, HPS-80-H high-dose intervention) at two time-points: pre-treatment before DSS administration and post-treatment after 7-day intervention. Genomic DNA extracted from fecal specimens was sequenced on the Illumina NovaSeq platform by Beijing Biomarker Technologies. Raw sequencing reads were processed using QIIME 2 for alpha-diversity, beta-diversity, taxonomic annotation, differential taxa identification and correlation analysis. This amplicon dataset provides raw sequencing resources for exploring how HPS-80 modulates gut microbiota to alleviate DSS-evoked colonic injury in mice.