Culture driven microbiome dynamics governs the hierarchical assembly of microbial cellulose, influence its structure, mechanics, and biological function
Dr. Gowri M. Balachander, Assistant Professor, School of Biomedical Engineering, Institute of Technology, Banaras Hindu University, Varanasi;Dr. Balamurugan Ramadass, Additional Professor, Department of Biochemistry, All India Institute of Medical Sciences, Bhubhaneswar.The aim of the study is to examine the composition and dynamics of the microbial community involved in microbial cellulose production, using SCOBY mediated fermentation in various culture media and cultivation conditions. The growth of the microbial communities derived from SCOBY in different media (HS, JCM, MTCC, and KTEA) under static and shaking conditions was performed, and 16S rRNA gene amplicon sequencing was used to characterize the microbial communities structure and successional patterns. Sequencing data was created to assess the impact of media composition and cultivation conditions on microbial diversity and taxonomic makeup as well as to investigate links between the microbial community dynamics and cellulose biosynthesis. The resulting microbiome data was combined with physiochemical and structural characterization of the microbial cellulose produced to explore the relationships between the microbial community composition, fermentation conditions, and properties of the cellulose material.