Project_Symphony_HiPCS_lines_genome_sequencing
Whole genome sequencing to verify genome integrity of wild-type and Cas13d expressing hiPSC lines used in project symphony.
A proof-of-concept CRISPR-Cas13d screen was performed in 3 lung cancer cell lines of varying genetic background. Selected hits identified from the CRISPR-Cas13d drop out screen, including protein coding genes and lncRNAs, were targeted in array to confirm target depletion, and identify associated downstream transcriptome changes to establish Cas13d specificity, lncRNA related novel biology and transcript specific depletion effects. Identified hits were verified in human induced stem cells to explore gene toxicity.