Transcriptome sequencing of Escherichia coli under different stress treatment conditions
Whole-transcriptome sequencing was performed to explore gene expression changes of E. coli NK5449 and E. coli MG1655 under various experimental treatments. Total RNA was extracted from bacterial samples, and paired-end RNA-seq libraries were constructed and sequenced on Illumina platform. Raw sequencing reads were obtained for subsequent transcriptomic analysis to identify differentially expressed genes.