CRISPRi genetic interaction screen of essential genes in M. tuberculosis (100ng/mL ATc)
A dual CRISPRi library focused on essential genes was transformed into M. tuberculosis The library was serially passaged in triplicate in either the presence (CRISPRi on) or absence (CRISPRi off) of ATc at a concentration of 100ng/mL. Genomic DNA was harvested at the following generations (cumulative) and subjected to deep sequencing: 0, 2.6, 5.4, 8.1, 10.8, 16.2, 21.6, 27.1, 32.5