Bisulfite amplicon sequencing (BSAS) of the HMOX1 and HMOX2 genes in Xinjiang Brown cattle under loose housing and tie stalls systems.
This research aims to characterize the differential impact of two housing systems (loose-housing and tie-stalls) on meat quality in Xinjiang Brown cattleand to elucidate the underlying epigenetic mechanisms, specifically DNA methylation. To this end, reduced representation bisulfite sequencing (RRBS) was first employed on the semitendinosus muscle, which identified the HMOX2 gene as being influenced by methylation. Subsequently, bisulfite amplicon sequencing (BSAS) was used to further validate the methylation status of this candidate gene.