Metabarcoding of the soil fungal community of Isla Navarino (Magallanes, Chile) using Illumina and Oxford Nanopore
This study took place in Isla Navarino, located within the Cape Horn Biosphere Reserve (UNESCO). We collected soil samples across the altitudinal gradient of Cerro Bandera, encompassing different vegetation assemblages. These samples were used for metabarcoding and sequencing on the Illumina NextSeq short-read platform (ITS2 and SSU) and the Oxford Nanopore MinION long-read platform (full ITS and partial LSU). This study aimed to characterize the soil fungal community associated with each vegetation assemblage along the gradient and to assess how Nanopore metabarcoding performs compared to the Illumina standard.