AAV capsid library screening by transcript-based sequencing in mice
This study evaluates transcript-based in vivo screening of an adeno-associated virus (AAV) capsid library in mice. An AAV library consisting of individually barcoded viral species was administered in vivo, and viral transcripts were recovered from mouse tissues for next-generation sequencing. The resulting sequencing data were used to quantify the relative abundance and recovery of individual library members across tissues and to assess the reproducibility and quantitative preservation of library representation following targeted viral transcript enrichment. This dataset provides sequencing-based measurements of AAV library-derived transcripts from mouse tissues and supports evaluation of transcript-based approaches for quantitative in vivo AAV capsid screening.