Oxford Nanopore transcriptomic profiling of mouse liver following a high-fat, high-fructose diet
This study investigated hepatic transcriptional alterations associated with high-fat, high-fructose diet-induced metabolic dysfunction-associated fatty liver disease. Male C57BL/6N mice were fed either a standard chow diet or a diet containing 60% fat together with fructose-supplemented drinking water at 42 g/L for 19 weeks. Full-length cDNA libraries prepared from liver RNA using the Oxford Nanopore Technologies cDNA-PCR Sequencing Kit (SQK-PCS109) were sequenced on the PromethION platform. The original experiment comprised eight independent libraries, with four biological replicates per dietary group. This SRA submission contains the two retained FASTQ files, including one chow-group library (WTchow) and one high-fat, high-fructose-group library (WT-HH). The FASTQ files and corresponding original signal data for the remaining six libraries are no longer available following expiration of the commercial sequencing provider's data-retention period and were confirmed to be unrecoverable. The transcriptomic data were generated to identify differentially expressed genes, particularly those involved in hepatic lipid transport and metabolism.