DNA aptamers selected against bacterial translation initiation factor 2 (IF2)
Bacterial translation initiation factor 2 (IF2) is a universally conserved GTPase that promotes recruitment and accommodation of the initiator tRNA and accelerates joining of the 50S ribosomal subunit. To obtain molecules that target IF2, single-stranded DNA aptamers were selected against E. coli IF2 by SELEX. This study deposits the sequences of the selected aptamer candidates (Apt311 to Apt314). The aptamers bind IF2 with low micromolar affinity and were characterized for their effect on translation initiation complex formation, providing reversible ligands to probe IF2-dependent steps of bacterial translation initiation.
Bacterial translation initiation factor 2 (IF2) is a universally conserved GTPase that promotes recruitment and accommodation of the initiator tRNA and accelerates joining of the 50S ribosomal subunit. To obtain molecules that target IF2, single-stranded DNA aptamers were selected against E. coli IF2 by SELEX. This study deposits the sequences of the selected aptamer candidates (Apt311 to Apt314). The aptamers bind IF2 with low micromolar affinity and were characterized for their effect on translation initiation complex formation, providing reversible ligands to probe IF2-dependent steps of bacterial translation initiation.