Maternal antibiotic exposure disrupts microbiota and exacerbates hyperoxia-induced lung injury in neonatal mice
Perinatal antibiotic treatment disrupts commensal microbiota and aggravates hyperoxia-induced neonatal lung injury. In this study, timed-pregnant C57BL/6N mice received maternal antibiotic treatment (MAT) in drinking water from gestational day 15 until delivery. Neonatal mice were exposed to room air (RA, 21% O2) or hyperoxia (85% O2) from postnatal days 1 to 7 across four groups: Control+RA, Control+O2, MAT+RA, and MAT+O2. On postnatal day 7, 16S rRNA amplicon sequencing was performed on samples from the left lung and lower gastrointestinal tract to determine the impact of MAT and hyperoxia on neonatal lung and gut microbial communities.