Regulbass : RNA-seq sequencing of European sea bass (Dicentrarchus labrax) lines differing in feed efficiency for Regulbass project, 2013.
This project investigates gene expression in European sea bass (Dicentrarchus labrax) to identify mechanisms linked to feed efficiency, a key trait in aquaculture. Individuals selected for RNA-seq analysis came from a population of 2000 tagged Dicentrarchus labrax housed in a single tank. Specific growth rate (SGR) was measured over two 3-week ad libitum feeding periods, followed by two cycles of 3-week fasting and 3-week refeeding. Average body mass loss during fasting was used to identify individuals with contrasting feed efficiency phenotypes (J+ and J- ), selected as being at least 1.5 standard deviations from the population mean. Total RNA was extracted from liver tissue using TRIzol reagent (Invitrogen), following the manufacturers protocol. RNAs from three individuals were pooled in equal amounts to generate each sample. A total of 35 samples were sequenced using paired-end 150 bp reads (2  150 bp) to an Illumina Genome Analyzer.
This project investigates gene expression in European sea bass (Dicentrarchus labrax) to identify mechanisms linked to feed efficiency, a key trait in aquaculture. Individuals selected for RNA-seq analysis came from a population of 2000 tagged Dicentrarchus labrax housed in a single tank. Specific growth rate (SGR) was measured over two 3-week ad libitum feeding periods, followed by two cycles of 3-week fasting and 3-week refeeding. Average body mass loss during fasting was used to identify individuals with contrasting feed efficiency phenotypes (J+ and J- ), selected as being at least 1.5 standard deviations from the population mean. Total RNA was extracted from liver tissue using TRIzol reagent (Invitrogen), following the manufacturers protocol. RNAs from three individuals were pooled in equal amounts to generate each sample. A total of 35 samples were sequenced using paired-end 150 bp reads (2  150 bp) to an Illumina Genome Analyzer.