Sleep fragmentation frequency is associated with distinct immune, microbial, and barrier-related profiles in DSS-induced colitis in mice
This BioProject contains raw paired-end 16S rRNA gene amplicon sequencing data from fecal samples collected at the experimental endpoint from male C57BL/6 mice. Mice were assigned to five groups: untreated control, DSS-induced colitis, DSS plus low-frequency sleep fragmentation (60 sweeps/h), DSS plus high-frequency sleep fragmentation (240 sweeps/h), and high-frequency sleep fragmentation alone. Colitis was induced with 2% dextran sulfate sodium for 7 days, and sleep fragmentation was applied for 12 h/day during the light phase. The V3-V4 region of bacterial 16S rRNA genes was sequenced to characterize gut microbial community composition and evaluate associations between sleep fragmentation frequency, DSS-induced colitis, and microbiota-related immune and intestinal barrier profiles.