Thalassiosira gravida microbiomes
The Arctic Diatom Thalassiosira gravida was grown in a multifactorial experiment with two levels of temperature (9°C, 13.5°C) and photoperiod (16 h, 24 h) under xenic and axenic conditions. When cultures reached mid-exponential growth phase (~15,000 cells per mL), cultures were filtered onto 10µm filters to retain their attached microbiome community. Samples were prepared for sequencing the 16S V4 region using the primers MS_v4_515F_N and MS_v4_806R_1. 16S rDNA libraries were sequenced on a NextSeq 2000 instrument (Illumina, San Diego, CA, United States) using the P1 Reagents kit with 2 x 300 cycles (paired end).
The Arctic Diatom Thalassiosira gravida was grown in a multifactorial experiment with two levels of temperature (9°C, 13.5°C) and photoperiod (16 h, 24 h) under xenic and axenic conditions. When cultures reached mid-exponential growth phase (~15,000 cells per mL), cultures were filtered onto 10µm filters to retain their attached microbiome community. Samples were prepared for sequencing the 16S V4 region using the primers MS_v4_515F_N and MS_v4_806R_1. 16S rDNA libraries were sequenced on a NextSeq 2000 instrument (Illumina, San Diego, CA, United States) using the P1 Reagents kit with 2 x 300 cycles (paired end).