The multigenerational effects of preconceptional administration of Chinese herbal medicine on male offspring in a murine model of polycystic ovary syndrome
A PCOS murine model was established via prenatal anti-Mullerian hormone exposure. BSTJF was administered preconceptionally, and its effects were evaluated in zero generation mice and their first and second generation male offspring. Reproduction, glucolipid metabolism, and the intestinal milieu phenotypes of male offspring were analysed via histological staining, biochemical analysis, 16S rRNA sequencing, liquid chromatography mass spectrometry untargeted metabolomics, and microbiota-metabolite correlation analysis.