Gene expression of human 3D epidermal equivalents exposed to low-humidity stress
Low humidity is a common environmental stress that perturbs epidermal homeostasis, yet the genome-wide transcriptional programs engaged in human keratinocytes under low-humidity conditions remain incompletely defined. To enable systematic analysis of keratinocyte responses to atmospheric dryness, we performed bulk RNA sequencing of reconstructed human 3D epidermal equivalents after 24 hours of low-humidity exposure and generated matched transcriptomes from cultures maintained under control-humidity conditions. This dataset provides transcriptome-wide expression profiles for comparative and integrative analyses of gene expression under low versus control humidity.