RNA Sequencing from U2OS HP1a Auxin Inducible Cell Line
We investigated the role of heterochromatin protein 1 alpha, a key chromatin crosslinking factor with affinity to H3K9me3 marks, in maintaining packing domain organization. Paradoxically, short-term degradation of heterochromatin protein a 1lpha resulted in overall downregulation of genes, contrasting with the canonical view that repression of heterochromatin allows chromatin to become more accessible for transcription.