Gene expression of mouse epidermis exposed to low versus control humidity in control mice (TRPV4 fl/fl) and TRPV4 cKO mice
Low humidity is a common environmental stress for skin, yet the early transcriptional responses of mammalian epidermis to acute atmospheric dryness and the contribution of TRPV4 remain incompletely defined. To enable comparative analyses of genotype- and environment-dependent gene expression, we performed bulk RNA sequencing of epidermal tissue isolated from wild-type and keratinocyte-specific TRPV4 conditional knockout (cKO) mice after 3 hours of exposure to either control humidity (50-60% relative humidity) or low humidity (<=20% relative humidity). This dataset provides transcriptome-wide expression profiles for integrative analyses of epidermal gene expression across humidity conditions and genotypes.