Haematococcus lacustris Raw sequence reads
Haematococcus pluvialis is the primary source of natural astaxanthin. However, there is an inherent antagonistic relationship between its growth and the synthesis of carotenoids, resulting in a relatively low nutritional biomass. To address this contradiction between growth and yield, a two-stage strategy assisted by methanol was developed. Methanol was used as a dual-functional substance that serves as both a carbon source supplement and a signaling molecule. This strategy overcame the growth-yield contradiction. 0.2% (v/v) of methanol significantly increased biomass by coordinating the upregulation of photosynthetic carbon fixation, cell cycle regulatory factors, and plant hormone signaling pathways, while shifting carbon metabolism from catabolism to anabolic pathways.