Whole-genome sequencing of Malaysian infectious bronchitis virus isolates
This BioProject contains raw Illumina MiSeq paired-end sequence data generated for two Malaysian infectious bronchitis virus (IBV) strains, IBS037A/2014 and IBS130/2015. The strains were isolated from chickens during IB outbreaks in Johor, Malaysia in 2014, and in Negeri Sembilan, Malaysia in 2015. The viruses were propagated in 9-day-old specific-pathogen-free embryonated chicken eggs, and allantoic fluid was harvested after 5 days of incubation at 37 degree Celcius. Viral RNA was extracted from infected allantoic fluid, reverse-transcribed, amplified as six overlapping RT-PCR fragments covering the complete IBV genome, and sequenced using the Illumina MiSeq platform. The data support complete genome assembly, genome annotation, and genomic analysis of Malaysian IBV strains.