Whole-genome sequencing of clotrimazole-resistant yeast
Saccharomyces cerevisiae strains (YPS163 background) of the following genotypes were evolved on clotrimazole-containing rich medium: the wild-type YJF153, HSC82 knockout, HSP82 knockout, hsp82-T101I, and hsp82-G313S. They were selected on 1.125 uM clotrimazole for 35 days and subsequently 2.25 uM clotrimazole for another 14 days. After the experimental evolution, we sequenced three to six populations per genotype, with two independent colonies sequenced per population. We found prevalent losses of mitochondrial DNA in the resistant colonies, along with a few point mutations and chromosomal duplications. Our findings provide insights into the genetic basis of clotrimazole resistance in the early phase of adaptation.