Metagenomics of Microcystis-associated bacterial enrichment cultures from El Loto urban wetland, Chile
This study presents shotgun metagenomic sequencing of bacterial enrichment cultures selectively obtained from Microcystis-dominated cyanobacterial colonies collected from El Loto urban wetland, a eutrophic freshwater system in the Los Ríos Region, southern Chile. Cyanobacterial colonies were concentrated by buoyancy-based centrifugation, propagated on BG-11 agar, and further cultured in BG-11 liquid medium to enrich for bacteria physically associated with Microcystis colonies. Five independent enrichment cultures were sequenced (two of which were additionally re-sequenced by the sequencing provider), and reads were co-assembled and binned to recover metagenome-assembled genomes (MAGs). Genome quality was assessed using CheckM2, and taxonomy was assigned using GTDB-Tk. This dataset includes raw paired-end Illumina reads and metagenome-assembled genomes (MAGs) spanning multiple bacterial phyla, including a MAG classified as Microcystis viridis and an unclassified Pseudanabaena sp. Data were generated to characterize the genome-resolved metabolic potential of colony-associated bacteria under laboratory enrichment conditions, with a focus on carbon fixation, nitrogen cycling, and photosynthesis-related pathways.
This study presents shotgun metagenomic sequencing of bacterial enrichment cultures selectively obtained from Microcystis-dominated cyanobacterial colonies collected from El Loto urban wetland, a eutrophic freshwater system in the Los Ríos Region, southern Chile. Cyanobacterial colonies were concentrated by buoyancy-based centrifugation, propagated on BG-11 agar, and further cultured in BG-11 liquid medium to enrich for bacteria physically associated with Microcystis colonies. Five independent enrichment cultures were sequenced (two of which were additionally re-sequenced by the sequencing provider), and reads were co-assembled and binned to recover metagenome-assembled genomes (MAGs). Genome quality was assessed using CheckM2, and taxonomy was assigned using GTDB-Tk. This dataset includes raw paired-end Illumina reads and metagenome-assembled genomes (MAGs) spanning multiple bacterial phyla, including a MAG classified as Microcystis viridis and an unclassified Pseudanabaena sp. Data were generated to characterize the genome-resolved metabolic potential of colony-associated bacteria under laboratory enrichment conditions, with a focus on carbon fixation, nitrogen cycling, and photosynthesis-related pathways.