Construction of engineered bacteria targeting P. copri and validation of in vitro effects
An engineered bacterium that can secrete a specific bacteriocin Lmo2776 was constructed by gene editing by CRISPER-cas9, and the ability to secrete bacteriocin was verified in vitro. Based on the above research basis, we selected high-fat mice as the model for in vivo validation to compare the differences in intestinal microbiota after gavage, and collected intestinal contents for 16s sequencing analysis, so as to discuss the potential therapeutic effect and mechanism of engineering bacteria on the HFD mice model.