FPLC separation of small RNAs from different pepper fruit tissues
Pepper (Capsicum annuum) small RNAs were extracted from three fruit tissues (flesh, placenta, seed) at 28 DAA and were separated with FPLC. The input, High Molecular Weight (HMW), Low Molecular Weight (LMW), and free fractions were collected, small RNAs were purified from them and sequenced on Illumina platform. The abundance of previously identified miRNAs and other sRNA sequences were measured in the different fractions to assess their RISC-loading efficiencies.