Direct RNA sequencing analysis of Carassius auratus herpesvirus
This study characterizes the transcript architecture of Carassius auratus herpesvirus (CaHV) using Oxford Nanopore direct RNA sequencing. Direct RNA sequencing of CaHV-JS-infected cells was used to define viral transcript boundaries, identify polyadenylation-associated cleavage sites, refine viral mRNA and coding sequence annotations, and identify previously unannotated open reading frames.