16S rRNA MiSeq sequencing of caecal faecal DNA from IRlox/lox vs PACIRKO mice to assess gut dysbiosis
The purpose of this study was to determine whether the loss of insulin-mediated pancreatic antimicrobial peptide (AMP) secretion into the gut from mice lacking pancreatic acinar insulin receptors (PACRIKO) leads to altered gut microbial diversity compared to their littermate control mice (IRlox/lox). 16S rRNA MiSeq sequencing was used to assess relative bacterial genera composition of the caecal faecal DNA of PACIRKO vs IRlox/lox mice. The V3 and V4 variable regions of 16S rRNA were amplified using 16S Amplicon PCR primers and sequenced using the Illumina MiSeq system (2x300 bp). Alpha-diversity, assessed by Shannon's H-index and Gini-Simpson index, was significantly reduced in faecal microbial DNA from PACIRKO mice (n=15) compared to IRlox/lox mice (n=11). Specifically, the butyrate-producing Lactobacillus genera was significantly reduced in PACIRKO mice. These data suggest that insulin-mediated pancreatic AMP secretion into the gut leads to reduced bacterial diversity with reduced butyrate-producing Lactobacillus bacteria.
The purpose of this study was to determine whether the loss of insulin-mediated pancreatic antimicrobial peptide (AMP) secretion into the gut from mice lacking pancreatic acinar insulin receptors (PACRIKO) leads to altered gut microbial diversity compared to their littermate control mice (IRlox/lox). 16S rRNA MiSeq sequencing was used to assess relative bacterial genera composition of the caecal faecal DNA of PACIRKO vs IRlox/lox mice. The V3 and V4 variable regions of 16S rRNA were amplified using 16S Amplicon PCR primers and sequenced using the Illumina MiSeq system (2x300 bp). Alpha-diversity, assessed by Shannon's H-index and Gini-Simpson index, was significantly reduced in faecal microbial DNA from PACIRKO mice (n=15) compared to IRlox/lox mice (n=11). Specifically, the butyrate-producing Lactobacillus genera was significantly reduced in PACIRKO mice. These data suggest that insulin-mediated pancreatic AMP secretion into the gut leads to reduced bacterial diversity with reduced butyrate-producing Lactobacillus bacteria.