TRAC-seq analysis of tRNA m7G modification remodeling induced by IGF2BP3 knockout
N7-methylguanosine modification in transfer RNAs represents a critical layer of epitranscriptomic regulation that governs translational efficiency and fidelity. As a well-characterized m7G reader protein, IGF2BP3 participates in multiple aspects of RNA metabolism, yet its regulatory impact on the global landscape of tRNA m7G modification remains largely unexplored. In this study, we employed TRAC-seq to profile genome-wide patterns of tRNA m7G modification in IGF2BP3-knockout cells and control cells. We systematically identified tRNA loci with significantly altered m7G modification levels and characterized the distribution patterns and functional relevance of these differentially modified tRNA species. This study reveals the regulatory role of IGF2BP3 in shaping the tRNA m7G modification landscape and provides epitranscriptomic evidence for further understanding the m7G regulatory network and the biological functions of IGF2BP3.