RNA-seq analysis of tRNA expression alterations in IGF2BP3-knockout T cells
Transfer RNAs are core mediators of protein translation, and their expression profiles directly shape translational output and cellular functional states. T cell activation and effector functions rely on tightly regulated protein synthesis, while the regulatory mechanism governing tRNA expression in T cells remains incompletely understood. IGF2BP3 is a conserved RNA binding protein involved in multiple RNA metabolic processes, but its impact on the global tRNA expression landscape in T cells has not been systematically characterized. In this study, we performed RNA sequencing on IGF2BP3-knockout T cells and wild-type control T cells to investigate the regulatory role of IGF2BP3 in tRNA expression. We quantified the genome-wide abundance of all tRNA species, identified significantly differentially expressed tRNAs, and analyzed their potential functional relevance. This study reveals the regulatory effect of IGF2BP3 on tRNA expression in T cells, and provides new evidence for understanding the post-transcriptional regulatory network of T cell immune function.