Liquid swine manure as a co-substrate and alkalinizing agent in sugarcane vinasse biodigestion: Kinetic aspects and microbial characterization
This study investigated the use of liquid swine manure (LSM) as a co-substrate and alkalinizing agent in the anaerobic digestion of raw and fermented sugarcane vinasse. Different vinasse-to-LSM proportions were evaluated to assess methane production, process kinetics, alkalinity, and microbial community structure. Microbial characterization was performed for both Bacteria and Archaea using 16S rRNA gene amplicon sequencing. The V3-V4 region was targeted for Bacteria using the 341F/806R primers, while the V4 region was targeted for Archaea using the Arch519F/Arch915R primers. Sequencing was performed on an Illumina NovaSeq 6000 platform using a 250 bp paired-end strategy. The resulting reads were processed using QIIME 2 and DADA2 for quality filtering, error correction, chimera removal, and amplicon sequence variant (ASV) inference. Taxonomic classification was performed using the MiDAS 5.3 reference database. The dataset provides information on bacterial and archaeal community composition associated with different substrate conditions and contributes to understanding the microbial responses involved in anaerobic digestion of sugarcane vinasse supplemented with liquid swine manure.