Ribo-seq analysis of translational efficiency remodeling induced by IGF2BP3 knockout in T cells
Translational regulation represents a pivotal post-transcriptional layer that governs T cell activation, proliferation and effector function, allowing rapid and precise adjustment of protein synthesis in response to immune stimuli. IGF2BP3 is a well-recognized RNA binding protein that participates in multiple steps of mRNA metabolism, but its global impact on translational efficiency in T cells remains largely unclear. In this study, we performed Ribo sequencing on IGF2BP3 knockout T cells and wild-type control cells to systematically characterize the translatome changes and evaluate the regulatory effect of IGF2BP3 on genome-wide translational efficiency. We identified genes with significantly altered translational efficiency upon IGF2BP3 deletion, and performed functional enrichment analysis to explore the biological processes and signaling pathways associated with these translationally dysregulated genes. This study defines the regulatory function of IGF2BP3 in controlling translational efficiency in T cells, and provides translatomic insights into the post-transcriptional regulatory mechanism of T cell immune responses.