Integrated full-length and short-read skin transcriptomics of Chinese spiny frog during Proteus mirabilis infection
This project investigates the skin transcriptional response of the Chinese spiny frog (Quasipaa spinosa) to Proteus mirabilis infection. A pooled PacBio Iso-Seq library was generated from control and infected skin RNA to establish a full-length transcript reference and resolve transcript isoforms. In parallel, six independent Illumina RNA-seq libraries were generated from three control and three infected frogs to characterize infection-associated gene expression changes. The project provides a transcript-level resource for studying skin defence, immune signalling and secretory or immune-effector candidates in Q. spinosa.