glia-targeted scRNA-Seq of hermaphrodite and male C. elegans during larval development
During C. elegans larval development, many genes undergo oscillatory patterns of gene expression, previously identified with bulk RNA-Seq (see e.g. Meeuse et al., 2020). This dataset uses scRNA-Seq to follow gene expression in multiple cell types during larval development, focusing on the L4 stage, using FACS enrichment of glia (otherwise underrepresented in whole-animal datasets), in particular ILso glia.Samples were synchronized by bleaching, and worms collected in L4 (mainly), L2 and adult stages. Glial cell types were enriched by FACS, targeting either all glia (mir-228prom), ILso glia (glr-18prom), or sheath glia (mam-5prom, 1 sample). Sorts were performed either for hermaphrodites, or male-enriched samples.