Gut microbiome sequencing of individuals across the HIV-TB spectrum with longitudinal follow-up of HIV+LTBI+ individuals during antiretroviral therapy
Gut microbiome composition was analyzed using 16S rRNA gene amplicon sequencing targeting the V3-V4 hypervariable regions on the Illumina MiSeq platform with 2x300 bp paired-end reads, generated from fecal samples of individuals across the HIV-TB disease spectrum, including HIV+LTBI+, HIV+LTBI-, HIV+TB+, and HIV-LTBI+ groups, with longitudinal sampling of HIV+LTBI+ during ART. These data were generated to investigate gut microbiome composition, diversity, and longitudinal changes associated with immune reconstitution and TB status.