eDNA metabarcoding (16S/18S) of offshore and inshore estuarine water for the ROME project, 2020-2023
This study provides an environmental DNA (eDNA) dataset generated within the French ROME pilot observatory network. From 2020 to 2023, every two weeks, water samples were collected at eight sampling sites (inshore estuarine stations influenced by anthropogenic inputs and offshore station) across four French coastal ecosystems: Rade de Brest, Marennes-Oleron, Baie des Veys, and the Thau Lagoon. Metabarcoding (16S/18S rDNA) were used to characterize bacterial and protist communities. Sequencing was performed on Illumina MiSeq. The dataset documents spatial and temporal variability in coastal microbiomes and supports the development of integrated, standardized microbial monitoring tools for estuarine environments. The dataset includes raw sequences, environmental metadata (temperature, salinity), and sample-level documentation, providing a unified resource for characterizing coastal microbiomes across contrasting estuarine environments.
This study provides an environmental DNA (eDNA) dataset generated within the French ROME pilot observatory network. From 2020 to 2023, every two weeks, water samples were collected at eight sampling sites (inshore estuarine stations influenced by anthropogenic inputs and offshore station) across four French coastal ecosystems: Rade de Brest, Marennes-Oleron, Baie des Veys, and the Thau Lagoon. Metabarcoding (16S/18S rDNA) were used to characterize bacterial and protist communities. Sequencing was performed on Illumina MiSeq. The dataset documents spatial and temporal variability in coastal microbiomes and supports the development of integrated, standardized microbial monitoring tools for estuarine environments. The dataset includes raw sequences, environmental metadata (temperature, salinity), and sample-level documentation, providing a unified resource for characterizing coastal microbiomes across contrasting estuarine environments.