Rumen metagenomic responses to different probiotics in Holstein calves
This BioProject contains metagenomic sequencing data from rumen fluid of Holstein calves supplemented with Bacillus subtilis, Saccharomyces cerevisiae, or their combination. Twenty-four rumen fluid samples were collected from four treatment groups, including a control group receiving a basal starter diet, a Bacillus subtilis-supplemented group, a Saccharomyces cerevisiae-supplemented group, and a combined supplementation group. The dataset was generated to characterize treatment-associated changes in rumen microbial community composition and functional potential, including microbial taxa, KEGG pathways, and carbohydrate-active enzyme profiles, and to provide insights into the microbial responses associated with rumen development in calves.