Gut microbiota profiling (16S rRNA) in DSS-induced colitis mice with Clostridium butyricum pretreatment
To investigate gut microbiota alterations induced by Clostridium butyricum pretreatment in a DSS-induced colitis mouse model, microbial DNA was extracted from colonic contents. The 16S rRNA gene amplicons were sequenced and used for community composition analysis across experimental groups. Amplicons targeting the V3-V4 region were sequenced on an Illumina MiSeq platform (paired-end).