16S rRNA gene sequencing of gut bacterial communities in Japanese sea perch
Ammonia-nitrogen stress is a major threat to the health and production of farmed Japanese sea perch (Lateolabrax japonicus). This study aims to characterize shifts in intestinal bacterial communities under ammonia-nitrogen stress and evaluate the regulatory effects of paeonol supplementation on gut microbiota. Using 16S rRNA gene (V3-V4 region) amplicon sequencing, we intend to reveal microbial compositional and diversity alterations across different experimental groups. These findings will provide basic microbiological evidence for applying paeonol as a potential protective agent against ammonia-nitrogen toxicity in Japanese sea perch aquaculture.