Suppression of cell migration by non-contact transspatial regulation function of the extracellular matrix via RAC1 signaling
Previously, we identified a non-canonical function of the extracellular matrix (ECM), termed non-contact transspatial regulation (nCTR), whereby the ECM influences cell behavior at a distance without direct physical contact. However, the validity of nCTR remains to be fully established, and its key determinants and molecular basis require further investigation. Here, we develop a custom-designed microfluidic platform that enables precise control of ECM-cell separation. Using this platform, we validate nCTR and show that the magnitude of this regulatory effect depends on ECM-cell spatial distance and matrix concentration. Transcriptomic profiling and inhibition experiment demonstrate that RAC1 signaling plays an important role in the nCTR-mediated suppression of cell migration. Together, our findings can serve as a crucial reference for future studies of the molecular mechanisms underlying non-contact ECM-cell communication.