RNA-seq of human breast cancer cell lines: CEBPB-TMEM160 regulation of PI3K/AKT pathway
This BioProject contains paired-end raw RNA-seq reads generated from human triple-negative breast cancer SUM159 cell line. Stable cell models with TMEM160 overexpression and TMEM160 knockdown together with their corresponding vector and scramble controls were constructed for transcriptomic profiling. The transcriptome data were applied to screen differentially expressed genes and uncover downstream signaling cascades mediated by TMEM160. GO and KEGG enrichment analyses revealed the involvement of PI3K/AKT signaling and epithelial-mesenchymal transition (EMT). The study aims to decipher the oncogenic function of TMEM160 and its transcriptional activation by transcription factor CEBPB in breast cancer progression. mRNA libraries were prepared and sequenced on Illumina NovaSeq platform by Novogene Bioinformatics Institute.