Whole genome sequencing and comparative genomics of Bacillus sp. BKSM4, a putative novel species isolated from Thai Pla-ra fermented fish
Bacillus sp. BKSM4 is a proteolytic halotolerant bacterium isolated from traditional Pla-ra (fermented freshwater fish) in Thailand. Whole genome sequencing was performed using Illumina paired-end technology and assembled using Unicycler v0.4.8. The genome is 3.97 Mb with 41.44% GC content assembled into 58 contigs (N50 = 263,736 bp; 100% completeness, 0.31% contamination by CheckM2). Genome-scale analyses (ANIb 94.84%, dDDH 62.2% vs closest relative Bacillus zhangzhouensis DW5-4) support classification as a putative novel species. This project includes the WGS genome assembly and raw Illumina sequencing reads.