Effects of Streptococcus thermophilus GRX02 on gut microbiota in a high-fat diet-induced mouse model
Male C57BL/6J mice aged 6 weeks were randomly divided into three groups: a normal diet (ND) group, a high-fat diet (HFD; 45% fat from diet) group, and a GRX02 group fed an HFD with oral administration of 1E8 CFU of live Streptococcus thermophilus GRX02 once daily. Over the 12-week experimental period, the effects of GRX02 on diet-induced obesity were evaluated. Fecal samples were collected one week prior to the end of the experiment, and the V3-V4 region of the 16S rRNA gene was amplified and sequenced to determine the structural and compositional changes in the gut microbiota.