Phosphatase inhibition leads to mutagenic outcomes during cisplatin-ICL repair
Next-generation sequencing was performed to identify the mutational spectra resulting from replication of a model plasmid containing a cisplatin-ICL in Xenopus egg extracts supplemented with pan-phosphatase inhibitor. Replication products were purified from extracts, and amplicons were generated by PCR and submitted to Azenta for paired-end next-generation sequencing (Illumina MiSeq, 2x250 bp).