Effects of methylmercury and vitamin A exposure on Wistar rats gut microbiota across generations
Animals were exposed during gestation and lactation to methylmercury and retinyl palmitate, a vitamin A precursor. Cecal samples from F0 dams and F1 offspring at weaning, on postnatal day 21 (F1-PND21). These samples were sequenced using IonTorrent platform.For long-term and multigenerational analysis, fecal samples were also collected from F1 and F2 animals at one year of age, corresponding to F1-PND365 and F2-PND365, respectively. These samples were sequenced using Illumina platform.