MCF7 Cells Treated with MDA-MB-231 Conditioned Media with and without Erlotinib Treatment
MCF7 human breast cancer cells were cultured in vitro and treated with conditioned medium collected from MDA-MB-231 triple-negative breast cancer cells. RNA was extracted following treatment for bulk RNA sequencing to assess transcriptional changes induced by paracrine signaling. Untreated MCF7 cells were used as controls. In a subset of conditions, cells were treated with erlotinib in combination with conditioned medium or prior to exposure, to evaluate transcriptional effects of EGFR inhibition under conditioned medium stimulation.