Paired 16S rRNA and ITS amplicon sequencing of topsoil microbiomes from mulberry (Morus alba) plantations in Sichuan, China
This BioProject provides paired bacterial (16S rRNA gene, V3-V4) and fungal (ITS1) amplicon sequencing data generated from topsoil (0-10 cm, 10-20 cm, 20-30 cm) collected beneath mulberry (Morus alba) trees at two plantation sites in Sichuan Province, China: Zigong and Nanchong. Sampling followed a nested design of two sites x three plots x three replicates, yielding 54 soil samples. For each sample, DNA was extracted once and used for both 16S rRNA and ITS amplification, so that bacterial and fungal profiles are directly paired at the sample level. Libraries were sequenced on the Illumina platform in paired-end mode, giving 108 sequencing results in total.