Root exudate components in shaping soil bacterial community structure and biodegradation potential
This project investigates how artificial root exudates composed of primarymetabolites alone or primary plus secondary metabolites shape soil bacterialcommunities and their responses to organochlorine contamination. Grassland soilmicrocosms were amended with artificial root exudates or water before exposureto chlorophenol or polychlorinated biphenyl mixtures. Bacterial communities werecharacterized before and after contamination using paired-end 16S rRNA geneV4-V5 amplicon sequencing. DNA stable isotope probing with 13C-labeled2-chlorophenol and 4-chlorobiphenyl was used to identify populations assimilatingpollutant-derived carbon. The deposit includes biological samples, microbialmock-community controls, and PCR no-template controls.