RNA-seq of Rattus norvegicus testis in LOH model and specific therapeutic intervention
The testis is the cornerstone of male spermatogenesis and androgen synthesis, and its functional decline is a hallmark of late-onset hypogonadism (LOH) associated with aging. This study aims to investigate the transcriptomic landscape of rat Sprague-Dawley testis across three distinct experimental conditions to evaluate the therapeutic efficacy of AIS (aromatase inhibitor) intervention. We collected testicular tissues from three groups: (1) normal healthy controls, (2) aging-induced LOH model rats, and (3) LOH model rats subjected to AIS treatment. High-throughput RNA-sequencing was performed to compare global gene expression profiles, identify differentially expressed genes (DEGs), and elucidate the key signaling pathways involved in testicular aging and endocrine recovery. This dataset will not only deepen our understanding of the molecular mechanisms underlying age-related testicular dysfunction but also provide a valuable reference for evaluating the transcriptomic effects of aromatase inhibitor-based therapy in male reproductive health.