Transcriptome changes in pig intramuscular fat cells after adding kynurenine
To directly evaluate the role of kynurenine in ectopic fat deposition, primary porcine intramuscular adipocytes were treated with kynurenine. Increased lipid droplet accumulation was observed in the cells, indicating the proadipogenic effect of kynurenine. Transcriptomic analysis further confirmed robust activation of the kynurenine AHR signaling module receptor and transportrelated genes including AHR, AHRR and SLC7A8 were upregulated, accompanied by elevated expression of the adipogenic regulatory genes LPL and LPIN1.