Dissecting tissue and pathogen divergent immune landscapes in Sebastes schlegelii against photobacteriosis and vibriosis via comparative transcriptomics
The specific objectives were as follows: (1) compare the pathogenicity of Pdp and Vh against S. schlegelii and characterize the global gene expression profiles of the head kidney and spleen at 6 and 12 h post-infection; (2) identify differentially expressed genes (DEGs) and systematically compare shared and unique DEG sets across tissues, pathogens and infection time points; (3) classify immune related DEGs and characterize the GO terms, KEGG pathways and WGCNA co-expression modules associated with each infection condition; and screen potential immune molecules and core pathways that may serve as targets for novel immunoprophylactic strategies.