Identification of miRNA Targets in Hop (Humulus lupulus L.) in Response to Verticillium nonalfalfae Infection Using Degradome Sequencing
Degradome (PARE) sequencing and analysis approach was chosen to capture miRNA derived cleavage products to understand the biological significance of miRNA-mediated regulation during pathogen infection. Roots of hop plants were artificially inoculated with Verticillium nonalfalfae fungi or submerged in water only (control plants). Roots were collected in different time points (3, 6, 10 and 14 days after inoculation) and pooled in four libraries: two for each hop cultivar (susceptible Celeia and resistant Wye-Target cultivar), one from mock-inoculated (control) plants and one from V. nonalfalfae-inoculated (treated) plants. Degradome sequencing of four libraries was performed using Illumina HiSeq X Sequencing System.