Third-generation full-length 16S rRNA gene sequencing of fecal microbiota in dairy goats under heat stress and melatonin treatment
This study investigated the effects of heat stress and melatonin treatment on the fecal microbiota of dairy goats using third-generation full-length 16S rRNA gene sequencing. Fecal samples were collected from dairy goats assigned to a normal control group (NC), a heat-stressed group (HS), and a melatonin-treated heat-stressed group (MT). Full-length 16S rRNA gene sequencing was performed to characterize changes in gut microbial composition, diversity, and taxonomic structure among the three groups. The dataset provides insights into heat stress-induced alterations of the intestinal microbiota and the potential modulatory effects of melatonin in dairy goats.