Metatranscriptomes from Experimental Models of Polymicrobial Wound Infections
Here, we analyzed the accuracy of different laboratory infection models of human chronic wounds. For the models, we had different community composition types as described:i) A monoculture of Staphylococcus aureus LAC inoculated into Wound Like Media (WLM) in 96-well deep well plates grown aerobically at 37 degree C for 48Hours (Sa_wlm).ii) A four-member community of S. aureus LAC, Pseudomonas aeruginosa PA01, Enterococcus faecalis V583, and Finegoldia magna SY01 inoculated into WLM in 96-well deep-well plates grown aerobically at 37 degree C for 48Hours (4mem_wlm). The same community was also inoculated into the dorsal skin wound of 8 to 10-week-old female C57BL/6J mice and harvested at four days post-infection (4mem_4dpi).iii) A seven-member community of S. aureus LAC, P. aeruginosa PA01, F. magna SY01, Streptococcus agalactiae A909, Cutibacterium acnes SK137, Anaerococcus hydrogenalis MJR7738A, and Corynebacterium amycolatum SK46 inoculated into WLM in 96-well deep-well plates grown aerobically at 37 degree C for 48Hours (7mem_wlm).iv) A seven-member community of S. aureus LAC, S. agalactiae A909, P. aeruginosa PA01, F. magna SY01, C. acnes SK137, A. hydrogenalis MJR7738A, and C. amycolatum SK46 at a ratio of 2:2:1:10:10:10 was used. The inoculum was inoculated into WLM (7mem_staggered) and WLM + 4.5g/L glucose (7mem_gluc) in 96-well deep-well plates grown aerobically at 37 degree C for 48Hours. The same community was also inoculated into the dorsal skin wound of 8 to 10-week-old female C57BL/6J mice and harvested at four days post-infection (7mem_4dpi).v) A six-member community of S. aureus LAC, F. magna SY01, S. agalactiae A909, C. acnes SK137, A. hydrogenalis MJR7738A, and C. amycolatum SK46 inoculated into WLM in 96-well deep-well plates grown aerobically at 37 degree C for 48Hours (6mem_wlm).