Marine microorganisms 16S
This project aims to investigate the dynamic changes in the diversity and community composition of marine microorganisms during laboratory cultivation. Seawater samples were collected from the coastal waters of Qinhuangdao, Hebei Province, China, in 2025. The samples were subsequently cultivated in the laboratory, and subsamples were collected at different time points throughout the culture process. Total genomic DNA was extracted from each subsample, and the V4-V5 region of the 16S rRNA gene was amplified and sequenced using high-throughput sequencing. The sequencing data were used to analyze the shifts in microbial community diversity and structure over the course of cultivation. This study provides insights into the succession of marine microbial communities under laboratory conditions and supports the isolation, screening, and utilization of marine microbial resources.