RNA-seq analysis during common bean seed development in the cultivars AB136 and MDRK
Differential gene expression analysis for red seed coat color was performed using the parents of the ABM population, ABM136 and MDRK. Plants were grown until physiological maturity in a greenhouse under controlled conditions to minimize environmental variability. Seeds were collected at two developmental stages and classified according to the progression of seed coat color changes. T0 corresponded to immature seeds in which pigmentation had initiated around the hilum, with a slight pink coloration distributed across the seed coat, whereas T1 corresponded to fully red seeds at the beginning of physiological maturity. The experimental design included three biological replicates for each stage and genotype.
Differential gene expression analysis for red seed coat color was performed using the parents of the ABM population, ABM136 and MDRK. Plants were grown until physiological maturity in a greenhouse under controlled conditions to minimize environmental variability. Seeds were collected at two developmental stages and classified according to the progression of seed coat color changes. T0 corresponded to immature seeds in which pigmentation had initiated around the hilum, with a slight pink coloration distributed across the seed coat, whereas T1 corresponded to fully red seeds at the beginning of physiological maturity. The experimental design included three biological replicates for each stage and genotype.