Whole genome sequencing of spontaneous gross chromosomal rearrangements (GCR) in the absence of AP endonucleases in an S. cerevisiae model system
Whole genome sequencing experiment to determine the structure of GCRs in the following mutants: apn1 apn2, sgs1, apn1 apn2 sgs1, apn1 apn2 sgs1 rev3. The GCR isolates were obtained using a counter-selectable marker system on the left arm of chromosome V. One canr 5-FOAr colony was selected per plating event for each genotype, grown to saturation and normalized by optical density before combining into pools of 3-5 isolates per pooled sample. High molecular weight genomic DNA was extracted using Yeastar DNA purification kit from Zymogen, quantified and submitted to Plasmidsaurus for long-read whole genome sequencing by Oxford Nanopore Technologies.