Effects of meconium FMT from sFGR and MCDA twins on antibiotic-pretreated mice
This project investigates the effects of fecal microbiota transplantation (FMT) using meconium samples from monochorionic diamniotic (MCDA) twins with selective fetal growth restriction (sFGR). Meconium from sFGR-affected twins and healthy MCDA twin controls was transplanted into antibiotic-pretreated mice to deplete the endogenous microbiota. Phosphate-buffered saline (PBS) was used as a negative control for FMT. 16S rRNA gene amplicon sequencing was performed on mouse cecal contents to evaluate microbial engraftment and compositional changes. This study aims to elucidate the potential role of meconium-derived microbiota in the pathogenesis of sFGR and their functional impacts on host metabolism and physiology.