Transcriptome analysis of Phoebe bournei under colchicine treatments with varying concentrations and soaking durations
This project provides RNA-seq transcriptome data of Phoebe bournei for polyploid induction research. Cold-stratified germinated seeds were soaked in mixed solutions containing five concentration gradients of colchicine (0.2 %, 0.4 %, 0.6 %, 0.8 %, 1.0 %) and 2 % DMSO for three soaking durations (24 h, 48 h, 72 h). Control groups were set up: untreated seeds (MCK0) and seeds immersed in distilled water for 24 h, 48 h and 72 h (MCK24/MCK48/MCK72). All treated seeds were cultivated into seedlings afterwards, and seedling root tips were collected as sequencing materials. RNA sequencing was performed on fresh root tip samples of Phoebe bournei seedlings subjected to different colchicine treatment concentrations and durations, to elucidate the molecular regulatory mechanism of polyploid formation induced by colchicine in Phoebe bournei.