Gene expression analysis of retinal pigment epithelial cells in hypoxia
This study compared physiological hypoxia with HIF-PHI-induced HIF stabilization in ARPE-19 cells to evaluate the extent to which pharmacological HIF activation reproduces hypoxia-induced transcriptional, metabolic, and angiogenic responses. RNA sequencing was used to profile and compare pathway-level changes under both conditions, with emphasis on HIF signaling, metabolic reprogramming, and angiogenic regulation. The study further builds on this approach by directly examining overlapping and distinct regulatory mechanisms between physiological hypoxia and HIF-PHI treatment in RPE cells.