Genome-wide RNA-Seq analysis of microRNAs level under the action of melanocortin analogue Semax under transient MCAO rat model conditions
The model of transient cerebral focal ischemia was used. It was realized on two-month-old male white rats of Wistar line. Animals were divided into two groups: "sham operation" and "ischemia". Focal ischemia in experimental rats from "ischemia" was initiated by endovascular occlusion of right middle cerebral artery using monofilament for 90 minutes followed by reperfusion. Rats were withdrawn from the experiment 24 hours after the occlusion. Ischemic focus was localized in the cerebral subcortex. The rats were injected 100 microg/kg intraperitoneally three times with melanocortin analogue Semax peptide (MEHFPGP, ACTH(4-7)PGP, Semax) at 90 min, as well as 2.5 and 6.5 hours after tMCAO. There were 3 animals in the group. Genome-wide transcriptome analysis of microRNAs level in rat subcortex of rats was carried out using high-throughput RNA-sequencing (RNA-Seq). microRNA fraction from total RNA was used for RNA-seq. Sequencing was carried out using an Illumina HiSeq 2500 instrument. At least 5 million reads (1/50 nt) were generated.In addition, there were control samples, namely the "sham-operation after saline administration" (SH24) and "tMCAO after saline administration" (IR24). These samples are registered in the project PRJNA596629.