A phased telomere-to-telomere genome of Rosa wichuraiana 'Basye's Thornless', a key progenitor for modern rose breeding
we present a telomere-to-telomere (T2T) haplotype-resolved genome assembly of BT generated using PacBio HiFi long reads, Oxford Nanopore Technologies ultra-long reads, and Hi-C chromatin conformation capture data. The final assembly spans 978.2 Mb and is anchored to 16 contiguous scaffolds, representing 14 chromosome-scale sequences corresponding to two complete haplotypes of seven chromosome pairs with six gaps, alongside the chloroplast and mitochondrial genomes. The N50 values of contig and scaffold are 67.9 Mb and 68.1 Mb, respectively. Assessment of assembly quality revealed a BUSCO completeness score of 99.0%, a consensus quality value (QV) of 68.11, and functional annotation of 95.6% among 67,611 predicted protein-coding genes, collectively confirming a high-quality, reference-grade assembly.
we present a telomere-to-telomere (T2T) haplotype-resolved genome assembly of BT generated using PacBio HiFi long reads, Oxford Nanopore Technologies ultra-long reads, and Hi-C chromatin conformation capture data. The final assembly spans 978.2 Mb and is anchored to 16 contiguous scaffolds, representing 14 chromosome-scale sequences corresponding to two complete haplotypes of seven chromosome pairs with six gaps, alongside the chloroplast and mitochondrial genomes. The N50 values of contig and scaffold are 67.9 Mb and 68.1 Mb, respectively. Assessment of assembly quality revealed a BUSCO completeness score of 99.0%, a consensus quality value (QV) of 68.11, and functional annotation of 95.6% among 67,611 predicted protein-coding genes, collectively confirming a high-quality, reference-grade assembly.