Fecal microbiota 16S rRNA sequencing of aged donors and FMT recipients in a neonatal overfeeding mouse model
This dataset comprises raw 16S rRNA gene sequencing reads from a study on Fecal Microbiota Transplantation (FMT) in an aging mouse model. We first employed an established neonatal overfeeding model by reducing litter size, assigning litters to either 4 pups per dam (Overfed group) or 8 pups per dam (Normal-fed control group). For the FMT experiment, donor fecal material was collected from 24-month-old normal-fed or overfed mice and transplanted into 12-month-old recipients maintained strictly on a standard chow diet (rNormal-Chow and rOver-Chow), fecal samples were prospectively collected at the end of the intervention. The data is presented in FASTQ format, representing 32 biological samples with consistent sequencing depth and high taxonomic resolution.