Identification and functional characterization of novel microRNAs from human umbilical cord mesenchymal stem cell-derived extracellular vesicles
Extracellular vesicles were isolated from conditioned medium of cultured human umbilical cord-derived mesenchymal stem cells. RNA extracted from three independent biological extracellular-vesicle preparations was analyzed by small RNA sequencing on an Illumina NovaSeq 6000 using single-end 50-base reads. Standard analysis identified three candidate novel microRNAs, novel_81, novel_208, and novel_238. Independent functional experiments provided evidence of anti-inflammatory activity for these candidates in the tested experimental system.
Extracellular vesicles were isolated from conditioned medium of cultured human umbilical cord-derived mesenchymal stem cells. RNA extracted from three independent biological extracellular-vesicle preparations was analyzed by small RNA sequencing on an Illumina NovaSeq 6000 using single-end 50-base reads. Standard analysis identified three candidate novel microRNAs, novel_81, novel_208, and novel_238. Independent functional experiments provided evidence of anti-inflammatory activity for these candidates in the tested experimental system.