Time-course RNA-seq analysis in root and shoot tissues of zinc-deficient Triticum aestivum cv. Paragon (bread wheat)
The aim of this study was to investigate transcriptional changes underlying the zinc (Zn) deficiency response in root and shoot tissues of bread wheat. Triticum aestivum cv. Paragon seedlings were hydroponically grown and subjected to Zn starvation (0 µM ZnCl2) starting at 14 days after germination. Samples were collected at 1, 3, 5, and 7 days following Zn withdrawal. RNA-seq was performed on whole root and shoot tissues, with three biological replicates per treatment and time point. Control plants supplied with 8 µM ZnCl2 were included to distinguish Zn deficiency transcriptional responses.
The aim of this study was to investigate transcriptional changes underlying the zinc (Zn) deficiency response in root and shoot tissues of bread wheat. Triticum aestivum cv. Paragon seedlings were hydroponically grown and subjected to Zn starvation (0 µM ZnCl2) starting at 14 days after germination. Samples were collected at 1, 3, 5, and 7 days following Zn withdrawal. RNA-seq was performed on whole root and shoot tissues, with three biological replicates per treatment and time point. Control plants supplied with 8 µM ZnCl2 were included to distinguish Zn deficiency transcriptional responses.