RNA sequencing of porcine intestinal epithelial cells infected with porcine epidemic diarrhea virus nsp3 Mac1 mutants
This study investigated the effects of mutations in the macrodomain (Mac1) of porcine epidemic diarrhea virus (PEDV) nonstructural protein 3 (nsp3) on host transcriptional responses in porcine intestinal epithelial cells. IPEC-J2 cells were infected with wild-type PEDV icPC22A or recombinant PEDV carrying the nsp3 Mac1 substitutions D445A or N462A at a multiplicity of infection of 3. Cells were collected at 12 hours post infection for RNA sequencing. The resulting RNA-seq dataset was generated to compare host transcriptional responses following infection with wild-type and nsp3 Mac1 mutant PEDV.