DNA methylation and whole-genome resequencing of 21 lotus samples
The unfolded leaf samples of 21 natural populations of Nelumbo nucifera were sampled. The collected samples were dehydrated with silica gel. For each sample, the total genomic DNA (gDNA) was extracted. High-quality DNA was carried out bisulfite conversion and used to construct the whole-genome bisulfite DNA libraries. These libraries wre sequenced on the Illumina HiSeq X10 platform to obtain paired-end 150 bp reads. Meanwhile, gDNA from each lotus sample was used for constructing the paired-end whole-genome resequencing DNA libraries with about 300 bp insert siez. The resequencing DNA libraires were sequenced on the Illumina NovaSeq 6000 platform.