ERR? inhibition in Crls1KO C2C12
Knockdown of Crls1 results in mitochondrial dysfunction and muscle fiber type-specific adaptation processes both in vivo. Here, we identified the transcription factor ERRgamma as a crucial effector of those processes and we now aim to validate the importance in vitro using differentiated C2C12 myotubes. To obtain the respective data we knocked down Crls1 and treated the cells with +/-GSK5182 (an ERRgamma inverse agonist). The aim is to capture the global transcriptional changes upon Crls1 knock down and GSK treatment.
Knockdown of Crls1 results in mitochondrial dysfunction and muscle fiber type-specific adaptation processes both in vivo. Here, we identified the transcription factor ERRgamma as a crucial effector of those processes and we now aim to validate the importance in vitro using differentiated C2C12 myotubes. To obtain the respective data we knocked down Crls1 and treated the cells with +/-GSK5182 (an ERRgamma inverse agonist). The aim is to capture the global transcriptional changes upon Crls1 knock down and GSK treatment.