AAV integration site analysis in human primary hepatocytes by target enrichment sequencing
Long-read target enrichment sequencing was performed on AAV6-eGFP-transduced primary human hepatocytes cultured in micropatterned co-culture and treated with ethyl methanesulfonate (EMS) to induce DNA damage. Integration sites were analyzed in untreated and EMS-treated samples collected 1-3 weeks after transduction to evaluate the effect of DNA damage on AAV vector integration frequency and distribution.